This page has only limited features, please log in for full access.
The cosmetic industry is a field on rise where the search for novel antioxidant ingredients, preferably from natural matrixes, became a new challenge. The reuse of Castanea sativa shells as a source of phytochemicals with pro-healthy effects in skin care products may be a valuable alternative to valorize this underexploited agro-industrial by-product. A previous study of our research group demonstrated the antioxidant properties of chestnut shells extract obtained by ultrasound-assisted extraction (UAE), as well as its safeness on skin cell lines, namely keratinocytes and fibroblasts. Based on the extract richness in antioxidants, a formulation containing C. sativa shells extract obtained by this environmentally friendly technology was successfully developed in the present study. The oil-in-water (O/W) cream incorporating the chestnut shells extract was further characterized regarding organoleptic and technological properties, namely color, pH, droplets size, and viscosity. A stability study over time was also accomplished. The results demonstrate that the formulation not only displayed pleasant organoleptic characteristics attractive to consumers but also a pH compatible with skin and a suitable viscosity for topical application. The stability study revealed minor changes to droplet size and color, without affecting the general stability of the formulation. In conclusion, this study emphasized the prominent benefits of chestnut shells extract as a novel ingredient for skin care formulations.
Diana Pinto; Fátima Lameirão; Cristina Delerue-Matos; Francisca Rodrigues; Paulo Costa. Characterization and Stability of a Formulation Containing Antioxidants-Enriched Castanea sativa Shells Extract. Cosmetics 2021, 8, 49 .
AMA StyleDiana Pinto, Fátima Lameirão, Cristina Delerue-Matos, Francisca Rodrigues, Paulo Costa. Characterization and Stability of a Formulation Containing Antioxidants-Enriched Castanea sativa Shells Extract. Cosmetics. 2021; 8 (2):49.
Chicago/Turabian StyleDiana Pinto; Fátima Lameirão; Cristina Delerue-Matos; Francisca Rodrigues; Paulo Costa. 2021. "Characterization and Stability of a Formulation Containing Antioxidants-Enriched Castanea sativa Shells Extract." Cosmetics 8, no. 2: 49.
Salicornia ramosissima J.Woods is an edible halophyte, widely distributed in the Portuguese salt marsh that has been under valorized. The aim of this study is to expand the knowledge regarding S. ramosissima bioactive composition and safety, highlighting its potential use as nutraceutical ingredient. Therefore, extracts obtained by conventional (CE) and microwave-assisted extraction (MAE) were characterized regarding phenolic profile, antioxidant activity, radical scavenging capacity and intestinal cell effects. Moreover, organic pesticides were screened to guarantee the consumers safety. The highest phenolic and flavonoid contents were observed for the CE, as well as the scavenging capacity of O2•− (IC50 = 979.36 µg/mL) and HOCl (IC50 = 90.28 µg/mL). In contrast, the best antioxidant and antiradical activities were achieved by MAE (65.56 µmol FSE/g dw and 17.74 µg AAE/g dw for FRAP and ABTS assays, respectively). The phenolic composition was similar for both extracts, being characterized by the presence of phenolic acids, flavonols, flavanols, flavones and flavanones. The predominant compound for both extracts was myricetin. None of the extracts were cytotoxic in intestinal cell lines. Vestigial levels of β-endosulfan and p,p’-DDE were identified in MAE. These results support that S. ramosissima could be a source of bioactive compounds for nutraceutic industry.
Ana Silva; João Lago; Diana Pinto; Manuela Moreira; Clara Grosso; Virgínia Cruz Fernandes; Cristina Delerue-Matos; Francisca Rodrigues. Salicornia ramosissima Bioactive Composition and Safety: Eco-Friendly Extractions Approach (Microwave-Assisted Extraction vs. Conventional Maceration). Applied Sciences 2021, 11, 4744 .
AMA StyleAna Silva, João Lago, Diana Pinto, Manuela Moreira, Clara Grosso, Virgínia Cruz Fernandes, Cristina Delerue-Matos, Francisca Rodrigues. Salicornia ramosissima Bioactive Composition and Safety: Eco-Friendly Extractions Approach (Microwave-Assisted Extraction vs. Conventional Maceration). Applied Sciences. 2021; 11 (11):4744.
Chicago/Turabian StyleAna Silva; João Lago; Diana Pinto; Manuela Moreira; Clara Grosso; Virgínia Cruz Fernandes; Cristina Delerue-Matos; Francisca Rodrigues. 2021. "Salicornia ramosissima Bioactive Composition and Safety: Eco-Friendly Extractions Approach (Microwave-Assisted Extraction vs. Conventional Maceration)." Applied Sciences 11, no. 11: 4744.
Castanea sativa is an outstanding species that represents a valuable natural resource for rural populations. C. sativa shells (CSS), an abundant agro-industrial by-product generated during chestnut peeling process, is commonly discarded or used as fuel. Nevertheless, CSS produced are not depleted by this application and huge amounts are still available, being particularly rich in bioactive compounds (polyphenols, vitamin E, lignin and oligosaccharides) with health benefits. Phytochemical studies reported not only antioxidant and antimicrobial activities, but also anti-inflammatory, anticancer, hypolipidemic, hypoglycemic and neuroprotective activities. The application of a suitable extraction technique is required for the isolation of bioactive compounds, being green extraction technologies outstanding for the industrial recovery of chestnut shells’ bioactive compounds. CSS were highlighted as remarkable sources of functional ingredients with promising applications in food and nutraceutical fields, mainly as natural antioxidants and effective prebiotics. This review aims to summarize the phytochemical composition and pro-healthy properties of CSS, emphasizing the sustainable extraction techniques employed in the recovery of bioactive compounds and their potential applications in food and nutraceutical industries.
Diana Pinto; María De La Luz Cádiz-Gurrea; Anna Vallverdú-Queralt; Cristina Delerue-Matos; Francisca Rodrigues. Castanea sativa shells: A review on phytochemical composition, bioactivity and waste management approaches for industrial valorization. Food Research International 2021, 144, 110364 .
AMA StyleDiana Pinto, María De La Luz Cádiz-Gurrea, Anna Vallverdú-Queralt, Cristina Delerue-Matos, Francisca Rodrigues. Castanea sativa shells: A review on phytochemical composition, bioactivity and waste management approaches for industrial valorization. Food Research International. 2021; 144 ():110364.
Chicago/Turabian StyleDiana Pinto; María De La Luz Cádiz-Gurrea; Anna Vallverdú-Queralt; Cristina Delerue-Matos; Francisca Rodrigues. 2021. "Castanea sativa shells: A review on phytochemical composition, bioactivity and waste management approaches for industrial valorization." Food Research International 144, no. : 110364.
During kiwiberry production, different by-products are generated, including leaves that are removed to increase the fruit’s solar exposure. The aim of this work was to extract bioactive compounds from kiwiberry leaf by employing microwave-assisted extraction (MAE). Compatible food solvents (water and ethanol) were employed. The alcoholic extract contained the highest phenolic and flavonoid contents (629.48 mg of gallic acid equivalents (GAE) per gram of plant material on dry weight (dw) (GAE/g dw) and 136.81 mg of catechin equivalents per gram of plant material on dw (CAE/g dw), respectively). Oppositely, the hydroalcoholic extract achieved the highest antioxidant activity and scavenging activity against reactive oxygen and nitrogen species (IC50 = 29.10 μg/mL for O2 •−, IC50 = 1.87 μg/mL for HOCl and IC50 = 1.18 μg/mL for •NO). The phenolic profile showed the presence of caffeoylquinic acids, proanthocyanidin, and quercetin in all samples. However, caffeoylquinic acids and quercetin were detected in higher amounts in the alcoholic extract, while proanthocyanidins were prevalent in the hydroalcoholic extract. No adverse effects were observed on Caco-2 viability, while the highest concentration (1000 µg/mL) of hydroalcoholic and alcoholic extracts conducted to a decrease of HT29-MTX viability. These results highlight the MAE potentialities to extract bioactive compounds from kiwiberry leaf.
Ana Silva; Diana Pinto; Iva Fernandes; Victor Freitas; María Cádiz-Gurrea; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. An Insight into Kiwiberry Leaf Valorization: Phenolic Composition, Bioactivity and Health Benefits. Molecules 2021, 26, 2314 .
AMA StyleAna Silva, Diana Pinto, Iva Fernandes, Victor Freitas, María Cádiz-Gurrea, Paulo Costa, Cristina Delerue-Matos, Francisca Rodrigues. An Insight into Kiwiberry Leaf Valorization: Phenolic Composition, Bioactivity and Health Benefits. Molecules. 2021; 26 (8):2314.
Chicago/Turabian StyleAna Silva; Diana Pinto; Iva Fernandes; Victor Freitas; María Cádiz-Gurrea; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. 2021. "An Insight into Kiwiberry Leaf Valorization: Phenolic Composition, Bioactivity and Health Benefits." Molecules 26, no. 8: 2314.
Olea europaea cultivar, native in the Mediterranean basin, has expanded worldwide, mainly due to the olive oil industry. This expansion is attributed to the benefits of olive oil consumption, since this product is rich in nutritional and bioactive compounds. However, the olive industry generates high amounts of wastes, which could be related to polluting effects on soil and water. To minimize the environmental impact, different strategies of revalorization have been proposed. In this sense, the aim of this work was to develop high cosmetic value added oleuropein-enriched extracts (O20 and O30), a bioactive compound from olive byproducts, performing a comprehensive characterization using high performance liquid chromatography coupled to mass spectrometry and evaluate their bioactivity by in vitro assays. A total of 49 compounds were detected, with oleuropein and its derivatives widely found in O30 extract, whereas iridoids were mainly detected in O20 extract. Moreover, 10 compounds were detected for the first time in olive leaves. Both extracts demonstrated strong antioxidant and antiradical activities, although O30 showed higher values. In addition, radical oxygen and nitrogen species scavenging and enzyme inhibition values were higher in O30, with the exception of HOCl and hyaluronidase inhibition assays. Regarding cell viability, olive byproduct extracts did not lead to a decrease in keratinocytes viability until 100 µg/mL. All data reported by the present study reflect the potential of industrial byproducts as cosmetic ingredients.
María Cádiz-Gurrea; Diana Pinto; Cristina Delerue-Matos; Francisca Rodrigues. Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study. Antioxidants 2021, 10, 245 .
AMA StyleMaría Cádiz-Gurrea, Diana Pinto, Cristina Delerue-Matos, Francisca Rodrigues. Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study. Antioxidants. 2021; 10 (2):245.
Chicago/Turabian StyleMaría Cádiz-Gurrea; Diana Pinto; Cristina Delerue-Matos; Francisca Rodrigues. 2021. "Olive Fruit and Leaf Wastes as Bioactive Ingredients for Cosmetics—A Preliminary Study." Antioxidants 10, no. 2: 245.
Hardy kiwifruit (Actinidia arguta) is a highly appreciated exotic fruit endowed with outstanding bioactive compounds. The present work proposes to characterize the pulp from A. arguta organic fruits, emphasizing its radicals scavenging capacity and effects on intestinal cells (Caco‐2 and HT29‐MTX). The physicochemical properties and phenolic profile were also screened. The total phenolic and flavonoid contents (TPC and TFC, respectively) of pulp were 12.21 mg GAE/g on dry weight (DW) and 5.92 mg CE/g DW, respectively. A high antioxidant activity was observed (FRAP: 151.41 μmol FSE/g DW; DPPH: 12.17 mg TE/g DW). Furthermore, the pulp did not induce a toxic effect on Caco‐2 and HT29‐MTX cells viability up to 1000 μg/mL. Regarding in vitro scavenging capacity, the pulp revealed the highest scavenging power against NO. (IC50=3.45 μg/mL) and HOCl (IC50=12.77 μg/mL). These results emphasize the richness of A. arguta fruit pulp to be used in different food products.
Diana Pinto; Stefania Sut; Stefano Dall'Acqua; Cristina Delerue‐Matos; Francisca Rodrigues. Actinidia arguta Pulp: Phytochemical Composition, Radical Scavenging Activity, and in Vitro Cells Effects. Chemistry & Biodiversity 2021, 18, 1 .
AMA StyleDiana Pinto, Stefania Sut, Stefano Dall'Acqua, Cristina Delerue‐Matos, Francisca Rodrigues. Actinidia arguta Pulp: Phytochemical Composition, Radical Scavenging Activity, and in Vitro Cells Effects. Chemistry & Biodiversity. 2021; 18 (3):1.
Chicago/Turabian StyleDiana Pinto; Stefania Sut; Stefano Dall'Acqua; Cristina Delerue‐Matos; Francisca Rodrigues. 2021. "Actinidia arguta Pulp: Phytochemical Composition, Radical Scavenging Activity, and in Vitro Cells Effects." Chemistry & Biodiversity 18, no. 3: 1.
Castanea sativa Mill. is a species of the family Fagaceae abundant in south Europe and Asia that represents an important resource in producing countries. During chestnut processing, a large amount of waste material is generated, namely leaves, flowers, shells, and burs, which are rich in bioactive compounds. These by-products represent a major environmental issue, and their reuse and valorization is advisable. Different biological activities, such as being antioxidants, have been associated with these compounds, highlighting their potential use in different fields such as pharmaceuticals, food, or cosmetics, generating more profits, reducing pollution costs, and improving social, economic, and environmental sustainability. The aim of this chapter is to provide knowledge about the type of chestnut by-products produced, the studies concerning their chemical composition and biological activity, and to discuss other possible applications of these materials.
Diana Pinto; Nair Braga; Ana Margarida Silva; Paulo Jorge Cardoso da Costa; Cristina Delerue-Matos; Francisca Rodrigues. Chestnut. Valorization of Fruit Processing By-products 2020, 127 -144.
AMA StyleDiana Pinto, Nair Braga, Ana Margarida Silva, Paulo Jorge Cardoso da Costa, Cristina Delerue-Matos, Francisca Rodrigues. Chestnut. Valorization of Fruit Processing By-products. 2020; ():127-144.
Chicago/Turabian StyleDiana Pinto; Nair Braga; Ana Margarida Silva; Paulo Jorge Cardoso da Costa; Cristina Delerue-Matos; Francisca Rodrigues. 2020. "Chestnut." Valorization of Fruit Processing By-products , no. : 127-144.
The objective of this study was to evaluate the optimal Subcritical Water Extraction (SWE) conditions of antioxidants and polyphenols from chestnut shells using Response Surface Methodology (RSM). A central composite design (CCD) was conducted to analyse the time (6–30 min) and temperature (51–249 °C) effects in antioxidant activity (ABTS, DPPH and FRAP) and Total Phenolic Compounds (TPC). TPC ranged from 315.21 to 496.80 mg gallic acid equivalents (GAE)/g DW; the DPPH from 549.23 to 1125.68 mg Trolox equivalents (TE)/g DW; ABTS varied between 631.16 and 965.45 mg ascorbic acid equivalents (AAE)/g DW and FRAP from 2793.95 to 11393.97 mg ferrous sulphate equivalents (FSE)/g DW. The optimal extraction conditions were 30 min/220 °C, revealing excelling scavenging efficiencies against HOCl (IC50 = 0.79 µg/mL) and O2− (IC50 = 12.92 µg/mL) without toxicity on intestinal cells (0.1 µg/mL). The phenolic composition revealed high amounts of pyrogallol and protocatechuic acid. SWE can be a useful extraction technique for the recovery of polyphenolics from chestnut shells.
Diana Pinto; Elsa F. Vieira; Andreia F. Peixoto; Cristina Freire; Vitor Freitas; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. Optimizing the extraction of phenolic antioxidants from chestnut shells by subcritical water extraction using response surface methodology. Food Chemistry 2020, 334, 127521 .
AMA StyleDiana Pinto, Elsa F. Vieira, Andreia F. Peixoto, Cristina Freire, Vitor Freitas, Paulo Costa, Cristina Delerue-Matos, Francisca Rodrigues. Optimizing the extraction of phenolic antioxidants from chestnut shells by subcritical water extraction using response surface methodology. Food Chemistry. 2020; 334 ():127521.
Chicago/Turabian StyleDiana Pinto; Elsa F. Vieira; Andreia F. Peixoto; Cristina Freire; Vitor Freitas; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. 2020. "Optimizing the extraction of phenolic antioxidants from chestnut shells by subcritical water extraction using response surface methodology." Food Chemistry 334, no. : 127521.
This work focused on evaluating the possibility of using vineyard pruning wastes from two Portuguese Vitis vinifera varieties; Touriga Nacional (TN) and Tinta Roriz (TR), as new potential ingredients for the nutraceutical industry. An environmentally friendly extraction technique; namely subcritical-water extraction (SWE), was employed. The overall results indicate that phenolic acids were the major class of compounds quantified; being gallic acid the principal one. The highest value for total phenolic content (TPC) was obtained for the TR extract at 250 °C (181 ± 12 mg GAE/g dw). In terms of antioxidant activity; the DPPH values for the extracts obtained at 250 °C were approximately 4-fold higher than the ones obtained at 125 °C; with TR extract presenting the highest value (203 ± 22 mg TE/g dw). Thus, the TR extract obtained through SWE at 250 °C was selected to evaluate the scavenging activity and the in vitro effects on cells due to the best results achieved in the previous assays. This extract presented the ability to scavenge reactive oxygen species (O2●-, HOCl and ROO●). No adverse effects were observed in HFF-1 viability after exposure to extract concentrations below 100 μg/mL. This work demonstrated that vine-canes extracts could be a potential ingredient to nutraceutical industry
Olena Dorosh; Manuela M. Moreira; Diana Pinto; Andreia F. Peixoto; Cristina Freire; Paulo Costa; Francisca Rodrigues; Cristina Delerue-Matos. Evaluation of the Extraction Temperature Influence on Polyphenolic Profiles of Vine-Canes (Vitis vinifera) Subcritical Water Extracts. Foods 2020, 9, 872 .
AMA StyleOlena Dorosh, Manuela M. Moreira, Diana Pinto, Andreia F. Peixoto, Cristina Freire, Paulo Costa, Francisca Rodrigues, Cristina Delerue-Matos. Evaluation of the Extraction Temperature Influence on Polyphenolic Profiles of Vine-Canes (Vitis vinifera) Subcritical Water Extracts. Foods. 2020; 9 (7):872.
Chicago/Turabian StyleOlena Dorosh; Manuela M. Moreira; Diana Pinto; Andreia F. Peixoto; Cristina Freire; Paulo Costa; Francisca Rodrigues; Cristina Delerue-Matos. 2020. "Evaluation of the Extraction Temperature Influence on Polyphenolic Profiles of Vine-Canes (Vitis vinifera) Subcritical Water Extracts." Foods 9, no. 7: 872.
The majority of works about vine-canes are focused on the evaluation of their chemical composition and antioxidant potential. To the best of our knowledge, the possible applications of produced extracts in cosmetic formulations have never been explored. The aim of the present study was to evaluate the antioxidant properties of vine-canes subcritical water extracts for use as active ingredients in the cosmetic industry. For that, the phenolic content and antioxidant activity of six vine-cane varieties, namely Alvarinho and Loureiro from the Minho region and Touriga Nacional and Tinta Roriz (TR) from both the Douro and Dão regions, were evaluated through spectrophotometric and chromatographic methods. All extracts presented similar antioxidant activity and the highest phenolic content was reported for TR variety from the Douro region (33.7 ± 1.9 mg GAE/g dw). The capacity of vine-cane extracts to capture reactive oxygen species superoxide (O2−) was also studied, with the highest IC50 value being obtained for Loureiro variety (56.68 ± 2.60 µg/mL). Furthermore, no adverse effects on HaCaT and HFF-1 dermal cell lines in concentrations below 100 and 1000 µg/mL, respectively, were determined. Finally, Loureiro vine-cane extract was incorporated into a topical formulation, and physical and microbiological properties were within expected values, demonstrating that vine-canes extracts can be successfully incorporated in cosmetic products.
Manuela M. Moreira; Francisca Rodrigues; Olena Dorosh; Diana Pinto; Paulo C. Costa; Jaroslava Švarc-Gajić; Cristina Delerue-Matos. Vine-Canes as a Source of Value-Added Compounds for Cosmetic Formulations. Molecules 2020, 25, 2969 .
AMA StyleManuela M. Moreira, Francisca Rodrigues, Olena Dorosh, Diana Pinto, Paulo C. Costa, Jaroslava Švarc-Gajić, Cristina Delerue-Matos. Vine-Canes as a Source of Value-Added Compounds for Cosmetic Formulations. Molecules. 2020; 25 (13):2969.
Chicago/Turabian StyleManuela M. Moreira; Francisca Rodrigues; Olena Dorosh; Diana Pinto; Paulo C. Costa; Jaroslava Švarc-Gajić; Cristina Delerue-Matos. 2020. "Vine-Canes as a Source of Value-Added Compounds for Cosmetic Formulations." Molecules 25, no. 13: 2969.
Hardy kiwi (Actinidia arguta) is a climbing, perennial and dioecious vine from Actinidiaceae family, native from Asia and valued as ornamental and traditional medicine. In the last decade, the growing interest as fruit-bearing plant encourage the expanding cultivation of A. arguta mainly to fruits production, particularly in Europe and North America. A. arguta plants have an extensive range of bioactive compounds that can be obtained from different botanical structures, such as fruits, leaves, flowers and stems. These bioactive molecules, with well-recognized health-promoting properties, include phenolic compounds, minerals, carbohydrates or even volatile substances, with a great potential to be used in several formulations of food products. Phytochemical studies on this plant reported hypoglycemic effects as well as antioxidant and anti-inflammatory activities, among others. The traditional uses of A. arguta have been experimentally proved by in vitro and in vivo studies, in which its bioactivities were associated to its phytochemical composition. This review aims to assess and summarize the phytochemical and healthy properties of the different botanical parts of A. arguta, describing their bioactive composition and exploring it potential functional properties on foodstuffs.
Diana Pinto; Cristina Delerue-Matos; Francisca Rodrigues. Bioactivity, phytochemical profile and pro-healthy properties of Actinidia arguta: A review. Food Research International 2020, 136, 109449 .
AMA StyleDiana Pinto, Cristina Delerue-Matos, Francisca Rodrigues. Bioactivity, phytochemical profile and pro-healthy properties of Actinidia arguta: A review. Food Research International. 2020; 136 ():109449.
Chicago/Turabian StyleDiana Pinto; Cristina Delerue-Matos; Francisca Rodrigues. 2020. "Bioactivity, phytochemical profile and pro-healthy properties of Actinidia arguta: A review." Food Research International 136, no. : 109449.
Castanea sativa shells (CSS), an abundant and underexploited agro-residue in chestnut producing countries, are an excellent source of bioactive compounds with outstanding health-promoting properties. The aim of this study was to recover bioactive compounds from CSS employing Supercritical Fluid Extraction-CO2 (SC-CO2). The optimization of the extraction was performed based on the antioxidant activity of CSS extracts evaluated by FRAP, DPPH and ABTS assays. The optimal CSS extract was further characterized regarding in vitro radical scavenging capacity, phenolic profile and intestinal cell effects. The optimal conditions were 60 °C, 350 bar and 15% of co-solvent. The results demonstrated that CSS extract was an effective scavenger of NO (IC50 = 0.76 μg/mL) and HOCl (IC50 = 1.57 μg/mL). The IC50 calculated for Caco-2 and HT29-MTX were, respectively, 477.94 μg/mL and 3.71 μg/mL. Indeed, the phenolic profile revealed the presence of phenolic acids (particularly ellagic acid and caffeic acid derivative) and flavonoids (epigallocatechin and catechin/epicatechin). Overall, this work suggests the valorization of CSS extracted by SC-CO2 as promising nutraceutical ingredient based on its bioactivity and safety. To the best of our knowledge, this is the first study that employed SC to recover bioactive compounds from CSS.
Diana Pinto; María De La Luz Cádiz-Gurrea; Stefania Sut; Ana Sofia Ferreira; Francisco Javier Leyva-Jimenez; Stefano Dall'Acqua; Antonio Segura-Carretero; Cristina Delerue-Matos; Francisca Rodrigues. Valorisation of underexploited Castanea sativa shells bioactive compounds recovered by supercritical fluid extraction with CO2: A response surface methodology approach. Journal of CO2 Utilization 2020, 40, 101194 .
AMA StyleDiana Pinto, María De La Luz Cádiz-Gurrea, Stefania Sut, Ana Sofia Ferreira, Francisco Javier Leyva-Jimenez, Stefano Dall'Acqua, Antonio Segura-Carretero, Cristina Delerue-Matos, Francisca Rodrigues. Valorisation of underexploited Castanea sativa shells bioactive compounds recovered by supercritical fluid extraction with CO2: A response surface methodology approach. Journal of CO2 Utilization. 2020; 40 ():101194.
Chicago/Turabian StyleDiana Pinto; María De La Luz Cádiz-Gurrea; Stefania Sut; Ana Sofia Ferreira; Francisco Javier Leyva-Jimenez; Stefano Dall'Acqua; Antonio Segura-Carretero; Cristina Delerue-Matos; Francisca Rodrigues. 2020. "Valorisation of underexploited Castanea sativa shells bioactive compounds recovered by supercritical fluid extraction with CO2: A response surface methodology approach." Journal of CO2 Utilization 40, no. : 101194.
Wine production generates large amounts of vine-canes, a devalued by-product that could be used for the recovery of bioactive compounds. In this work, two vine-canes varieties, namely Touriga Nacional (TN) and Tinta Roriz (TR), were submitted to different ultrasound-assisted extraction (UAE) conditions. The highest phenolic and flavonoid content was observed for TR extract obtained at lab-scale without an ice bath and pilot-scale after 60 min of extraction (32.6 ± 2.1 and 26.0 ± 1.5 mg gallic acid equivalent/g dry weight (dw) and 9.5 ± 0.6 and 8.3 ± 0.8 mg epicatechin equivalents/g dw, respectively). Further, all extracts demonstrated a high antioxidant activity to scavenge DPPH free radicals with the best value reached by TR at the lab-scale without an ice bath after 30 min and pilot-scale extraction after 60 min (34.2 ± 2.4 and 33.4 ± 2.1 mg trolox equivalents/g dw, respectively). Extracts phenolic composition were also evaluated by HPLC, demonstrating that resveratrol, myricetin and catechin were the main compounds. According to our knowledge, this is the first time that a pilot scale of UAE of phenolic compounds from vine-canes was performed. This paper represents an important step to the use of UAE as an industrial process to recover bioactive compounds.
Olena Dorosh; Manuela M. Moreira; Francisca Rodrigues; Andreia F. Peixoto; Cristina Freire; Simone Morais; Cristina Delerue-Matos. Vine-Canes Valorisation: Ultrasound-Assisted Extraction from Lab to Pilot Scale. Molecules 2020, 25, 1739 .
AMA StyleOlena Dorosh, Manuela M. Moreira, Francisca Rodrigues, Andreia F. Peixoto, Cristina Freire, Simone Morais, Cristina Delerue-Matos. Vine-Canes Valorisation: Ultrasound-Assisted Extraction from Lab to Pilot Scale. Molecules. 2020; 25 (7):1739.
Chicago/Turabian StyleOlena Dorosh; Manuela M. Moreira; Francisca Rodrigues; Andreia F. Peixoto; Cristina Freire; Simone Morais; Cristina Delerue-Matos. 2020. "Vine-Canes Valorisation: Ultrasound-Assisted Extraction from Lab to Pilot Scale." Molecules 25, no. 7: 1739.
Chestnut processing industry generates large amounts of by-products, including leaves, burs and shells that are a source of bioactive compounds. The purpose of this study was to establish an ultrasound-assisted extraction (UAE) of phenolic and antioxidant compounds from industrial chestnut shells. A central composite design (CCD) was conducted to analyze the effects of time (4–46 min) and temperature (34–76 °C) in the antioxidant activity (2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and ferric reducing antioxidant power (FRAP)) and total phenolic compounds (TPC) of chestnut shells extracts. The optimal extraction conditions were obtained at 70 °C for 40 min. The optimal extract was characterized regarding phenolic profile, radical scavenging capacity, and effects on intestinal and dermal cell lines. The optimal extract revealed high amounts of ellagic acid (40.4 µg/mg dw), followed by caffeic acid derivative (15.4 µg/mg dw) and epigallocatechin (15.3 µg/mg dw). Indeed, the extract exhibited the highest scavenging efficiencies against NO● (IC50 = 0.1 µg/mL) and HOCl (IC50 = 0.7 µg/mL) and did not conducted to a decrease on HaCaT and HFF-1 viability up to 100 μg/mL. Oppositely, a decrease on Caco-2 and HT29-MTX viability was observed. This study suggests that UAE could be a sustainable option to valorize chestnut shells as raw material for different industries.
Fátima Lameirão; Diana Pinto; Elsa F. Vieira; Andreia F. Peixoto; Cristina Freire; Stefania Sut; Stefano Dall’Acqua; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro. Antioxidants 2020, 9, 267 .
AMA StyleFátima Lameirão, Diana Pinto, Elsa F. Vieira, Andreia F. Peixoto, Cristina Freire, Stefania Sut, Stefano Dall’Acqua, Paulo Costa, Cristina Delerue-Matos, Francisca Rodrigues. Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro. Antioxidants. 2020; 9 (3):267.
Chicago/Turabian StyleFátima Lameirão; Diana Pinto; Elsa F. Vieira; Andreia F. Peixoto; Cristina Freire; Stefania Sut; Stefano Dall’Acqua; Paulo Costa; Cristina Delerue-Matos; Francisca Rodrigues. 2020. "Green-Sustainable Recovery of Phenolic and Antioxidant Compounds from Industrial Chestnut Shells Using Ultrasound-Assisted Extraction: Optimization and Evaluation of Biological Activities In Vitro." Antioxidants 9, no. 3: 267.
This study aimed to prepare and characterize organogels containing microparticles of ascorbic acid (AA) obtained from propolis by-product. The formulations F1 (5% of microparticles) and F2 (10% of microparticles) were evaluated regarding rheological and textural properties, antioxidant and radical scavenging activity, in vitro release and cellular studies. The organogels showed plastic flow behavior and rheopexy. The textural parameters were within acceptable values for semisolid formulations. The antioxidant capacity of organogels F1 and F2 by the DPPH assay demonstrated IC50 ranging from 1523.59 to 1166.97 μg/mL, respectively. For the FRAP assay, the values found were 842.88 and 956.14 μmol of FSE/g formulation, respectively. Good scavenging activity against nitrogen species was observed. The concentration of 63 μg/mL did not present toxicity on HaCaT and HFF-1 cells. In vitro release profile of AA from organogels showed a slow pattern of drug release, mainly for F2. Therefore, the proposed organogel containing AA microparticles with propolis by-product matrix represents a promising platform for topical drug delivery with antioxidant effect.
Lizziane Maria Belloto De Francisco; Diana Pinto; Hélen Cássia Rosseto; Lucas De Alcântara Sica De Toledo; Rafaela Said dos Santos; Paulo Jorge Cardoso da Costa; Beatriz Oliveira; Bruno Sarmento; Francisca Rodrigues; Marcos Luciano Bruschi. Design and characterization of an organogel system containing ascorbic acid microparticles produced with propolis by-product. Pharmaceutical Development and Technology 2019, 25, 54 -67.
AMA StyleLizziane Maria Belloto De Francisco, Diana Pinto, Hélen Cássia Rosseto, Lucas De Alcântara Sica De Toledo, Rafaela Said dos Santos, Paulo Jorge Cardoso da Costa, Beatriz Oliveira, Bruno Sarmento, Francisca Rodrigues, Marcos Luciano Bruschi. Design and characterization of an organogel system containing ascorbic acid microparticles produced with propolis by-product. Pharmaceutical Development and Technology. 2019; 25 (1):54-67.
Chicago/Turabian StyleLizziane Maria Belloto De Francisco; Diana Pinto; Hélen Cássia Rosseto; Lucas De Alcântara Sica De Toledo; Rafaela Said dos Santos; Paulo Jorge Cardoso da Costa; Beatriz Oliveira; Bruno Sarmento; Francisca Rodrigues; Marcos Luciano Bruschi. 2019. "Design and characterization of an organogel system containing ascorbic acid microparticles produced with propolis by-product." Pharmaceutical Development and Technology 25, no. 1: 54-67.
The development of functional and nutraceutical foods comes from a greater awareness of the relationship between food and health by consumers. In recent years, the idea of purifying and encapsulating bioactive compounds through techniques such as spray drying has been well received by the food industry. The development and characterization of a grapefruit (Citrus paradisi) nutraceutical powder obtained by spray drying is of great interest owing to the different bioactive compounds and the potential health effects. The grapefruit powder was characterized by a low water amount (1.5 g water per 100 g powder) and a high porosity (75%). The color parameters were L* = 80.0 ± 1.8, hab * = 61.7 ± 0.4 and Cab * = 11.4 ± 0.6. The IC50 values determined for the freeze-dried oxalic acid extract (FDOA) and the freeze-dried methanol-water extract (FDMW) were 0.48 and 0.72 mg mL-1 respectively, while the total phenolic content (TPC) ranged between 1274 and 1294 mg gallic acid equivalent (GAE) per 100 g dry basis (d.b.). Regarding total flavonoid content (TFC), FDOA presented the highest amount (6592 mg quercetin equivalent (QE) per 100 g d.b.). For both extracts, the cell viability in Caco-2 and HT29-MTX was above 90% at 100 μg mL-1 . The bioavailability of the bioactive compounds was analyzed through a 3D intestinal model. Delphenidin-3-glucoside and hesperitin-7-O-glucoside presented a permeation higher than 50%, followed by hesperidin which was close to 30%. This work allows to establish that the formulation of grapefruit powder has great potential as a nutraceutical food, with spray drying being a good alternative technique in the food industry. © 2019 Society of Chemical Industry.
Freddy González; Eva García‐Martínez; Mª Del Mar Camacho; Nuria Martínez-Navarrete; Bruno Sarmento; Iva Fernandes; Victor Freitas; Francisca Rodrigues; Beatriz Oliveira. Insights into the development of grapefruit nutraceutical powder by spray drying: physical characterization, chemical composition and 3D intestinal permeability. Journal of the Science of Food and Agriculture 2019, 99, 4686 -4694.
AMA StyleFreddy González, Eva García‐Martínez, Mª Del Mar Camacho, Nuria Martínez-Navarrete, Bruno Sarmento, Iva Fernandes, Victor Freitas, Francisca Rodrigues, Beatriz Oliveira. Insights into the development of grapefruit nutraceutical powder by spray drying: physical characterization, chemical composition and 3D intestinal permeability. Journal of the Science of Food and Agriculture. 2019; 99 (10):4686-4694.
Chicago/Turabian StyleFreddy González; Eva García‐Martínez; Mª Del Mar Camacho; Nuria Martínez-Navarrete; Bruno Sarmento; Iva Fernandes; Victor Freitas; Francisca Rodrigues; Beatriz Oliveira. 2019. "Insights into the development of grapefruit nutraceutical powder by spray drying: physical characterization, chemical composition and 3D intestinal permeability." Journal of the Science of Food and Agriculture 99, no. 10: 4686-4694.
In the present study, a coffee-like beverage was prepared from Quercus cerris seeds.
Diana Pinto; Santiago Diaz Franco; Ana Margarida Silva; Snezana Cupara; Marijana Koskovac; Ksenija Kojicic; Sónia Soares; Francisca Rodrigues; Stefania Sut; Stefano Dall'Acqua; M. Beatriz P. P. Oliveira. Chemical characterization and bioactive properties of a coffee-like beverage prepared from Quercus cerris kernels. Food & Function 2019, 10, 2050 -2060.
AMA StyleDiana Pinto, Santiago Diaz Franco, Ana Margarida Silva, Snezana Cupara, Marijana Koskovac, Ksenija Kojicic, Sónia Soares, Francisca Rodrigues, Stefania Sut, Stefano Dall'Acqua, M. Beatriz P. P. Oliveira. Chemical characterization and bioactive properties of a coffee-like beverage prepared from Quercus cerris kernels. Food & Function. 2019; 10 (4):2050-2060.
Chicago/Turabian StyleDiana Pinto; Santiago Diaz Franco; Ana Margarida Silva; Snezana Cupara; Marijana Koskovac; Ksenija Kojicic; Sónia Soares; Francisca Rodrigues; Stefania Sut; Stefano Dall'Acqua; M. Beatriz P. P. Oliveira. 2019. "Chemical characterization and bioactive properties of a coffee-like beverage prepared from Quercus cerris kernels." Food & Function 10, no. 4: 2050-2060.
Mushrooms are important sources of natural bioactive compounds that are increasingly used as cosmeceutical ingredients. In this context, ethanolic extracts were prepared from Ganoderma lucidum and Pleurotus ostreatus and tested by incorporation into base cosmetic creams. In vitro safety evaluation of the extracts and cosmetic formulations prepared therefore was carried out using the MTT and LDH assays in keratinocyte (HaCaT) and fibroblast (HFF-1) cell lines. Moreover, they were submitted to ex vivo skin permeation studies using a Franz diffusion apparatus with pig ear skin as permeation membrane. The results showed the absence of toxicity for keratinocytes and fibroblasts in a concentration dependent manner, which is indicative of the safety of these extracts for cosmeceutical ingredients purposes. Protocatechuic and syringic acids were the only compounds permeating from G. lucidum extract in the first 8 h of study, no penetration was observed for phenolic acids found in P. ostreatus extract and formulation. These results pointed out for the suitability of using mushroom extracts as skin care ingredients and may contribute for the valorisation of wastes generated by the mushroom processing industry, which can serve as raw-materials for the obtainment of the extracts.
Oludemi Taofiq; Francisca Rodrigues; Lillian Barros; Maria F. Barreiro; Isabel C.F.R. Ferreira; M. Beatriz P.P. Oliveira. Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies. Food and Chemical Toxicology 2019, 127, 228 -236.
AMA StyleOludemi Taofiq, Francisca Rodrigues, Lillian Barros, Maria F. Barreiro, Isabel C.F.R. Ferreira, M. Beatriz P.P. Oliveira. Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies. Food and Chemical Toxicology. 2019; 127 ():228-236.
Chicago/Turabian StyleOludemi Taofiq; Francisca Rodrigues; Lillian Barros; Maria F. Barreiro; Isabel C.F.R. Ferreira; M. Beatriz P.P. Oliveira. 2019. "Mushroom ethanolic extracts as cosmeceuticals ingredients: Safety and ex vivo skin permeation studies." Food and Chemical Toxicology 127, no. : 228-236.
Actinidia deliciosa and A. arguta fruits (kiwifruit and kiwiberry, respectively) are an excellent source of bioactive compounds. The aim of this paper is to valorize the fruits that are not commercialized (e.g. due to inadequate size or physical damage) in infusions and decoctions. The antioxidant activity, the scavenging activity against reactive species, the phenolic profile and the intestinal effects of infusions and decoctions of dehydrated fruits were evaluated and compared. Decoctions presented the highest antioxidant activity and a good ability to capture HOCl and •NO. The phenolic composition of A. arguta present quinic acid, cis-caftaric acid and its derivatives, caffeoyl hexoside, luteolin glucuronide, quercetin derivatives and myristin, while A. deliciosa extracts were characterized by the presence of quinic acid, caffeic acid and its derivatives and caffeoyl hexoside. No adverse effects were observed on Caco-2 and HT29-MTX cells. Kiwiberry decoctions showed to be the best option to keep the fruits benefits.
Ana Margarida Silva; Diana Pinto; Iva Fernandes; Tânia Gonçalves Albuquerque; Helena S. Costa; Vitor Freitas; Francisca Rodrigues; M. Beatriz P.P. Oliveira. Infusions and decoctions of dehydrated fruits of Actinidia arguta and Actinidia deliciosa: Bioactivity, radical scavenging activity and effects on cells viability. Food Chemistry 2019, 289, 625 -634.
AMA StyleAna Margarida Silva, Diana Pinto, Iva Fernandes, Tânia Gonçalves Albuquerque, Helena S. Costa, Vitor Freitas, Francisca Rodrigues, M. Beatriz P.P. Oliveira. Infusions and decoctions of dehydrated fruits of Actinidia arguta and Actinidia deliciosa: Bioactivity, radical scavenging activity and effects on cells viability. Food Chemistry. 2019; 289 ():625-634.
Chicago/Turabian StyleAna Margarida Silva; Diana Pinto; Iva Fernandes; Tânia Gonçalves Albuquerque; Helena S. Costa; Vitor Freitas; Francisca Rodrigues; M. Beatriz P.P. Oliveira. 2019. "Infusions and decoctions of dehydrated fruits of Actinidia arguta and Actinidia deliciosa: Bioactivity, radical scavenging activity and effects on cells viability." Food Chemistry 289, no. : 625-634.
Following our previous work on the antitumor activity of acetylated flavonosides, a new acetylated xanthonoside, 3,6-bis(2,3,4,6-tetra-O-acetyl-β-glucopyranosyl)xanthone (2), was synthesized and discovered as a potent inhibitor of tumor cell growth. The synthesis involved the glycosylation of 3,6-di-hydroxyxanthone (1) with acetobromo-α-d-glucose. Glycosylation with silver carbonate decreased the amount of glucose donor needed, comparative to the biphasic glycosylation. Xanthone 2 showed a potent anti-growth activity, with GI50 < 1 μM, in human cell lines of breast, lung, and glioblastoma cancers. Current treatment for invasive brain glioma is still inadequate and new agents against glioblastoma with high brain permeability are urgently needed. To overcome these issues, xanthone 2 was encapsulated in a liposome. To increase the well-known low stability of these drug carriers, a proliposome formulation was developed using the spray drying method. Both formulations were characterized and compared regarding three months stability and in vitro anti-growth activity. While the proliposome formulation showed significantly higher stability, it was at the expense of losing its biocompatibility as a drug carrier in higher concentrations. More importantly, the new xanthone 2 was still able to inhibit the growth of glioblastoma cells after liposome formulation.
Ana Alves; Marta Correia-Da-Silva; Claúdia Nunes; João Campos; Maria Emília Sousa; Patrícia M. A. Silva; Hassan Bousbaa; Francisca Rodrigues; Domingos Ferreira; Paulo C. Costa; Madalena Pinto. Discovery of a New Xanthone against Glioma: Synthesis and Development of (Pro)liposome Formulations. Molecules 2019, 24, 409 .
AMA StyleAna Alves, Marta Correia-Da-Silva, Claúdia Nunes, João Campos, Maria Emília Sousa, Patrícia M. A. Silva, Hassan Bousbaa, Francisca Rodrigues, Domingos Ferreira, Paulo C. Costa, Madalena Pinto. Discovery of a New Xanthone against Glioma: Synthesis and Development of (Pro)liposome Formulations. Molecules. 2019; 24 (3):409.
Chicago/Turabian StyleAna Alves; Marta Correia-Da-Silva; Claúdia Nunes; João Campos; Maria Emília Sousa; Patrícia M. A. Silva; Hassan Bousbaa; Francisca Rodrigues; Domingos Ferreira; Paulo C. Costa; Madalena Pinto. 2019. "Discovery of a New Xanthone against Glioma: Synthesis and Development of (Pro)liposome Formulations." Molecules 24, no. 3: 409.