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Fohad Mabood Husain
Department of Food Science and Nutrition, College of Food and Agriculture Science, King Saud University, Riyadh, Saudi Arabia

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Paper
Published: 04 August 2021 in RSC Advances
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Lactoferrin is a heme-binding multifunctional glycoprotein known for iron transportation in the blood and also contributes to innate immunity.

ACS Style

Mohd Shahnawaz Khan; Rais Ahmad Khan; Tabish Rehman; Mohamed A. Ismael; Fohad Mabood Husain; Mohamed F. AlAjmi; Majed S. Alokail; Nojood Altwaijry; Ali M. Alsalme. Elucidation of molecular interactions of theaflavin monogallate with camel milk lactoferrin: detailed spectroscopic and dynamic simulation studies. RSC Advances 2021, 11, 26710 -26720.

AMA Style

Mohd Shahnawaz Khan, Rais Ahmad Khan, Tabish Rehman, Mohamed A. Ismael, Fohad Mabood Husain, Mohamed F. AlAjmi, Majed S. Alokail, Nojood Altwaijry, Ali M. Alsalme. Elucidation of molecular interactions of theaflavin monogallate with camel milk lactoferrin: detailed spectroscopic and dynamic simulation studies. RSC Advances. 2021; 11 (43):26710-26720.

Chicago/Turabian Style

Mohd Shahnawaz Khan; Rais Ahmad Khan; Tabish Rehman; Mohamed A. Ismael; Fohad Mabood Husain; Mohamed F. AlAjmi; Majed S. Alokail; Nojood Altwaijry; Ali M. Alsalme. 2021. "Elucidation of molecular interactions of theaflavin monogallate with camel milk lactoferrin: detailed spectroscopic and dynamic simulation studies." RSC Advances 11, no. 43: 26710-26720.

Journal article
Published: 27 March 2021 in Processes
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Beetroot is a widely consumed crop all over the world and contains plenty of bioactive compounds. In this study, we analyzed the bioactive as well as the antimicrobial properties of the oven-dried beetroot (pulp and peel) using different solvents. The 50% methanolic extract yielded the highest content of total polyphenols and total flavonoids as well as the reducing power of the beetroot. The beetroot peel exhibited a higher content of total polyphenols, total flavonoids, and reducing power activity under all the extraction solvents. Mixture of methanol and water (50% v/v) extracted the highest chlorogenic acid (78.24 mg/100 g) of the dried beetroot peel, while 1,2-dihydroxybenzene was the most noteworthy phenolic compound (42.52 mg/100 g) in beetroot peel methanolic extract. The 50% methanolic extract of both peel and pulp demonstrated the highest antibacterial and anticandidal potential. These results can be helpful for beetroot consumption.

ACS Style

Ahmad Salamatullah; Khizar Hayat; Mohammed Alkaltham; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Alhanouf Al-Dossari; Ghalia Shamlan; Laila Al-Harbi. Bioactive and Antimicrobial Properties of Oven-Dried Beetroot (Pulp and Peel) Using Different Solvents. Processes 2021, 9, 588 .

AMA Style

Ahmad Salamatullah, Khizar Hayat, Mohammed Alkaltham, Mohammed Ahmed, Shaista Arzoo, Fohad Husain, Alhanouf Al-Dossari, Ghalia Shamlan, Laila Al-Harbi. Bioactive and Antimicrobial Properties of Oven-Dried Beetroot (Pulp and Peel) Using Different Solvents. Processes. 2021; 9 (4):588.

Chicago/Turabian Style

Ahmad Salamatullah; Khizar Hayat; Mohammed Alkaltham; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Alhanouf Al-Dossari; Ghalia Shamlan; Laila Al-Harbi. 2021. "Bioactive and Antimicrobial Properties of Oven-Dried Beetroot (Pulp and Peel) Using Different Solvents." Processes 9, no. 4: 588.

Journal article
Published: 01 February 2021 in Sustainability
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Fruits and vegetables constitute a considerable amount of antioxidants and among them eggplant is a rich source of polyphenol compounds. This study investigated the bioactive and antimicrobial properties of eggplant under different degree of microwave cooking. The eggplant was cooked for 7 min (light cooked), 10 min (medium cooked), and 15 min (high cooked). The highest total polyphenol content was observed in the light cooked eggplant sample (27.35 mg gallic acid equivalent (GAE)/g dry weight (DW)) followed by high cooked sample (26.10 mg GAE/g DW), while the lowest total polyphenol content (2.79 mg GAE/g DW) was obtained for the uncooked (control) sample. The total polyphenol content of the samples ranged in the following order; light cooked > high cooked > medium cooked > uncooked. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging of eggplant ranged between 16.16% (control) and 47.88% (high cooked). The highest reducing power was exhibited by the light cooked (absorbance 1.708) eggplant sample followed by the high cooked (absorbance 1.597), while the lowest reducing power was shown by uncooked sample (absorbance 0.389). Moreover, antimicrobial studies showed that light cooked eggplant sample demonstrated broad-spectrum inhibition of growth in Gram-positive as well as Gram-negative bacteria and Candida albicans. Slightly lower antimicrobial potential was exhibited by medium cooked eggplant sample while no antibacterial or antifungal activity was recorded for the extract of high cooked eggplant sample. Microwave cooking might be a method to enhance the antioxidant and antimicrobial potential of eggplant.

ACS Style

Ahmad Salamatullah; Mohammed Alkaltham; Khizar Hayat; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Abdulhakeem Alzahrani. Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking. Sustainability 2021, 13, 1519 .

AMA Style

Ahmad Salamatullah, Mohammed Alkaltham, Khizar Hayat, Mohammed Ahmed, Shaista Arzoo, Fohad Husain, Abdulhakeem Alzahrani. Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking. Sustainability. 2021; 13 (3):1519.

Chicago/Turabian Style

Ahmad Salamatullah; Mohammed Alkaltham; Khizar Hayat; Mohammed Ahmed; Shaista Arzoo; Fohad Husain; Abdulhakeem Alzahrani. 2021. "Bioactive and Antimicrobial Properties of Eggplant (Solanum melongena L.) under Microwave Cooking." Sustainability 13, no. 3: 1519.

Journal article
Published: 30 January 2021 in Biomolecules
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Green synthesis of metal nanoparticles using plant extracts as capping and reducing agents for the biomedical applications has received considerable attention. Moreover, emergence and spread of multidrug resistance among bacterial pathogens has become a major health concern and lookout for novel alternative effective drugs has gained momentum. In current study, we synthesized gold nanoparticles using the seed extract of Trachyspermum ammi (TA-AuNPs), assessed its efficacy against drug resistant biofilms of Listeria monocytogenes and Serratia marcescens, and evaluated its anticancer potential against HepG2 cancer cell lines. Microwave-assisted green synthesis of gold nanoparticles was carried out and characterization was done using UV-vis spectroscopy, X-ray diffraction (XRD), transmission electron microscopy (TEM), and dynamic light scattering (DLS). Most nanoparticles were observed as spherical and spheroidal with few anisotropies with an average crystalline size of 16.63 nm. Synthesized TA-AuNPs demonstrated significant biofilm inhibitory activity against L. monocytogenes (73%) as well as S. marcescens (81%). Exopolysaccharide (EPS), motility, and CSH, key elements that facilitate the formation and maintenance of biofilm were also inhibited significantly at the tested sub-minimum inhibitory concentrations (sub-MICs). Further, TA-AuNPs effectively obliterated preformed mature biofilms of S. marcescens and L. monocytogenes by 64% and 58%, respectively. Induction of intracellular ROS production in TA-AuNPs treated bacterial cells could be the plausible mechanism for the reduced biofilm formation in test pathogens. Administration of TA-AuNPs resulted in the arrest of cellular proliferation in a concentration-dependent manner. TA-AuNPs decrease the intracellular GSH in HepG2 cancer cell lines, cells become more prone to ROS generation, hence induce apoptosis. Thus, this work proposes a new eco-friendly and rapid approach for fabricating NPs which can be exploited for multifarious biomedical applications.

ACS Style

Kahkashan Perveen; Fohad Husain; Faizan Qais; Altaf Khan; Suhail Razak; Tayyaba Afsar; Pravej Alam; Ali Almajwal; Mahmoud Abulmeaty. Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity. Biomolecules 2021, 11, 197 .

AMA Style

Kahkashan Perveen, Fohad Husain, Faizan Qais, Altaf Khan, Suhail Razak, Tayyaba Afsar, Pravej Alam, Ali Almajwal, Mahmoud Abulmeaty. Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity. Biomolecules. 2021; 11 (2):197.

Chicago/Turabian Style

Kahkashan Perveen; Fohad Husain; Faizan Qais; Altaf Khan; Suhail Razak; Tayyaba Afsar; Pravej Alam; Ali Almajwal; Mahmoud Abulmeaty. 2021. "Microwave-Assisted Rapid Green Synthesis of Gold Nanoparticles Using Seed Extract of Trachyspermum ammi: ROS Mediated Biofilm Inhibition and Anticancer Activity." Biomolecules 11, no. 2: 197.

Original article
Published: 03 January 2021 in Applied Nanoscience
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Diabetes and its related complications are responsible for severe morbidity and deaths round the globe. Diabetic foot infections (DFIs) are severe and dreadful complication of diabetes mellitus. DFI/DFUs are colonized with numerous pathogenic, multi-drug-resistant microorganisms and progression in colonization of these microorganism leads to biofilm establishment making treatment challenging. There is an indispensable requirement for alternative approaches to combat this threat. In the present assignment, titanium oxide nanoparticles (TiO2-NPs) were fabricated from the leaf extract of Ochradenus arabicus and inhibition of biofilm and related functions was examined against multi-drug-resistant strains of P. aeruginosa and methicillin-resistant S. aureus (MRSA) isolated from foot ulcers. Bio-fabricated TiO2-NPs reduced biofilm formation in the selected strains by 22–70% at tested sub-MICs. Significant reduction in alginate and severely impaired motility of P. aeruginosa strains was also observed. Key functions such as cell surface hydrophobicity and EPS production that contribute in the attachment and maintenance of biofilm, respectively, were also inhibited considerably in test strains at sub-MICs. Statistically significant reduction (51–63%) in the preformed biofilms of all strains was also recorded. Interaction of bacterial cell and TiO2-NPs resulted in increased levels of ROS, and this could be the plausible the reason for the biofilm inhibitory action of NPs. These NPs could be exploited in the current treatment strategies to combat the threat of biofilm-based drug-resistant diabetic foot infections and facilitate wound healing.

ACS Style

Mohammad Zubair; Fohad Mabood Husain; Faizan Abul Qais; Pravej Alam; Iqbal Ahmad; Thamer AlBalawi; Naushad Ahmad; Manawwer Alam; Mohammad Hassan Baig; Jae-June Dong; Farha Fatima; Badr Alsayed. Bio-fabrication of titanium oxide nanoparticles from Ochradenus arabicus to obliterate biofilms of drug-resistant Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot infections. Applied Nanoscience 2021, 11, 375 -387.

AMA Style

Mohammad Zubair, Fohad Mabood Husain, Faizan Abul Qais, Pravej Alam, Iqbal Ahmad, Thamer AlBalawi, Naushad Ahmad, Manawwer Alam, Mohammad Hassan Baig, Jae-June Dong, Farha Fatima, Badr Alsayed. Bio-fabrication of titanium oxide nanoparticles from Ochradenus arabicus to obliterate biofilms of drug-resistant Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot infections. Applied Nanoscience. 2021; 11 (2):375-387.

Chicago/Turabian Style

Mohammad Zubair; Fohad Mabood Husain; Faizan Abul Qais; Pravej Alam; Iqbal Ahmad; Thamer AlBalawi; Naushad Ahmad; Manawwer Alam; Mohammad Hassan Baig; Jae-June Dong; Farha Fatima; Badr Alsayed. 2021. "Bio-fabrication of titanium oxide nanoparticles from Ochradenus arabicus to obliterate biofilms of drug-resistant Staphylococcus aureus and Pseudomonas aeruginosa isolated from diabetic foot infections." Applied Nanoscience 11, no. 2: 375-387.

Journal article
Published: 01 January 2021 in Saudi Journal of Biological Sciences
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Food producing animals harbouring bacteria carrying drug resistance genes especially the metallo-beta-lactamase (MBL) pose high risk for the human population. In addition, formation of biofilm by these drug resistant pathogens represents major threat to food safety and public health. In this study, metallo-β-lactamases (MβLs) producing Pseudomonas spp. from camel meat were isolated and assessed for their biofilm formation. Further, in vitro and in silico studies were performed to study the effect of flavone naringin on biofilm formation against isolated Pseudomonas spp. A total of 55% isolates were found to produce metallo-β-lactamase enzyme. Naringin mitigated biofilm formation of Pseudomonas isolates up to 57%. Disturbed biofilm architecture and reduced the colonization of bacteria on glass was observed under scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM). The biofilm related traits such as exopolysaccharides (EPS) and alginate production was also reduced remarkably in the presence of naringin. Eradication of preformed biofilms (32–60%) was also observed at the respective 0.50 × MICs. Molecular docking revealed that naringin showed strong affinity towards docked proteins with binding energy ranging from −8.6 to −8.8 kcal mol−1. Presence of metallo-β-lactamase producers indicates that camel meat could be possible reservoir of drug-resistant Pseudomonas species of clinical importance. Naringin was successful in inhibiting biofilm formation as well as eradicating the preformed biofilms and demonstrated strong binding affinity towards biofilm associated protein. Thus, it is envisaged that naringin could be exploited as food preservative especially against the biofilm forming food-borne Pseudomonas species and is a promising prospect for the treatment of biofilm based infections.

ACS Style

Fohad Mabood Husain; Kahkashan Perveen; Faizan Abul Qais; Iqbal Ahmad; Ahmed H. Alfarhan; Mohamed A. El-Sheikh. Naringin inhibits the biofilms of metallo-β-lactamases (MβLs) producing Pseudomonas species isolated from camel meat. Saudi Journal of Biological Sciences 2021, 28, 333 -341.

AMA Style

Fohad Mabood Husain, Kahkashan Perveen, Faizan Abul Qais, Iqbal Ahmad, Ahmed H. Alfarhan, Mohamed A. El-Sheikh. Naringin inhibits the biofilms of metallo-β-lactamases (MβLs) producing Pseudomonas species isolated from camel meat. Saudi Journal of Biological Sciences. 2021; 28 (1):333-341.

Chicago/Turabian Style

Fohad Mabood Husain; Kahkashan Perveen; Faizan Abul Qais; Iqbal Ahmad; Ahmed H. Alfarhan; Mohamed A. El-Sheikh. 2021. "Naringin inhibits the biofilms of metallo-β-lactamases (MβLs) producing Pseudomonas species isolated from camel meat." Saudi Journal of Biological Sciences 28, no. 1: 333-341.

Journal article
Published: 17 December 2020 in Molecules
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Epoxy resins (EP) have been used as a thermos-setting material in the field of coating, casting, bonding agent, and laminating. However, a major drawback associated with its use is the lack of good flaming properties, and it is responsible for heavy smoke along with hazardous gases considerably limiting its uses in various fields. In this study, N-ethanolamine triazine-piperizine, a melamine polymer (ETPMP), was established as a new charring-foaming agent and was successfully synthesized with ethanolamine, piperizine, cyanuric chloride, and melamine as precursor molecules via the nucleophilic substitution reaction method. Elemental analysis and Fourier transform infrared (FTIR) spectroscopy analysis were applied to approve the synthesis of ETPMP and confirmation of its structure and characterization. The epoxy coating of intumescent flame retardant (IFR) was equipped by introducing ETPMP, ammonium polyphosphate (APP), and copper oxide (CuO) in multiple composition ratios. CuO was loaded at various amounts into the IFR-coating system as a synergistic agent. The synergistic action of CuO on IFR coatings was scientifically examined by using different analytical tests such as vertical burning test (UL-94V), limited oxygen index (LOI), thermal gravimetric analysis (TGA), cone calorimeter, and scanning electron microscope (SEM). The results showed that small changes in the amount of CuO expressively amplified the LOI results and enhanced the V-0 ratings in the UL-94V test. The TGA data clearly demonstrate that the inclusion of CuO can transform the thermal deprivation behavior of coatings with a growing char slag proportion with elevated temperatures. Information from cone calorimeter data affirmed that CuO can decrease the burning factors by total heat release (THR) together with peak heat release rate (PHRR). The SEM images indicated that CuO can enrich the power and compression of the intumescent char that restricts the movement of heat and oxygen. Our results demonstrate a positive influence of CuO on the epoxy-headed intumescent flame retardant coatings.

ACS Style

Riyazuddin; Samrin Bano; Fohad Mabood Husain; Jamal Akhter Siddique; Khadijah H. Alharbi; Rais Ahmad Khan; Ali Alsalme. Role of Copper Oxide on Epoxy Coatings with New Intumescent Polymer-Based Fire Retardant. Molecules 2020, 25, 5978 .

AMA Style

Riyazuddin, Samrin Bano, Fohad Mabood Husain, Jamal Akhter Siddique, Khadijah H. Alharbi, Rais Ahmad Khan, Ali Alsalme. Role of Copper Oxide on Epoxy Coatings with New Intumescent Polymer-Based Fire Retardant. Molecules. 2020; 25 (24):5978.

Chicago/Turabian Style

Riyazuddin; Samrin Bano; Fohad Mabood Husain; Jamal Akhter Siddique; Khadijah H. Alharbi; Rais Ahmad Khan; Ali Alsalme. 2020. "Role of Copper Oxide on Epoxy Coatings with New Intumescent Polymer-Based Fire Retardant." Molecules 25, no. 24: 5978.

Journal article
Published: 16 December 2020 in International Journal of Biological Macromolecules
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Advanced glycation endproducts (AGEs) are the final product of glycation, highly reactive in nature and contribute directly or indirectly to numerous complications related to diabetes. In this study, the antiglycation activity of glyburide was investigated using HSA as model protein, both against glucose and methylglyoxal mediated glycation. The possible mechanism of action was also deciphered using biophysical and computational tools. Approximately 70% inhibition of both early and advanced glycation end products were recorded in the presence of glyburide. Free lysine modification was reduced by glyburide treatment and improvement in biochemical markers such as free thiol groups and carbonyl content was observed. Interaction studies revealed that glyburide showed moderate to strong binding affinity towards HSA with binding constant in the order of 106 M−1. The interaction of glyburide with HSA was entropically favourable and spontaneous in nature. Molecular dynamics simulation deciphered that glyburide-HSA complex was quite stable where RMSD, RMSF, Rg, SASA, and secondary structure of HSA remained approximately same over the entire simulation period. The average binding energy of the MD simulation for glyburide-HSA complex was found to be −15.386 kJ mol−1. The findings demonstrate the antiglycation potential of glyburide and its possible mechanism of action.

ACS Style

Faizan Abul Qais; Tarique Sarwar; Iqbal Ahmad; Rais Ahmad Khan; Syed Ali Shahzad; Fohad Mabood Husain. Glyburide inhibits non-enzymatic glycation of HSA: An approach for the management of AGEs associated diabetic complications. International Journal of Biological Macromolecules 2020, 169, 143 -152.

AMA Style

Faizan Abul Qais, Tarique Sarwar, Iqbal Ahmad, Rais Ahmad Khan, Syed Ali Shahzad, Fohad Mabood Husain. Glyburide inhibits non-enzymatic glycation of HSA: An approach for the management of AGEs associated diabetic complications. International Journal of Biological Macromolecules. 2020; 169 ():143-152.

Chicago/Turabian Style

Faizan Abul Qais; Tarique Sarwar; Iqbal Ahmad; Rais Ahmad Khan; Syed Ali Shahzad; Fohad Mabood Husain. 2020. "Glyburide inhibits non-enzymatic glycation of HSA: An approach for the management of AGEs associated diabetic complications." International Journal of Biological Macromolecules 169, no. : 143-152.

Journal article
Published: 08 December 2020 in Agriculture
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The presence of heavy metals in increased concentrations in the environment has become a global environmental concern. This rapid increase in heavy metals in the environment is attributed to enhanced industrial and mining activities. Metal ions possess a lengthy half-life and property to bioaccumulate, are non-biodegradable and, thus, are a threat to the human health. A number of conventional spectroscopic and chromatographic techniques are being used for the detection of heavy metals, but these suffer from various limitations. Nano-based sensors have emerged as potential candidates for the sensitive and selective detection of heavy metals. Thus, the present study was focused on the synthesis of selenium nanoparticles (SeNPs) by using selenite-reducing bacteria in the development of a heavy metal toxicity biosensor. During the biosynthesis of selenium nanoparticles, supernatants of the overnight-grown culture were treated with Na2SeO32− and incubated for 24 h at 37 °C. The as-synthesized nanoparticles were characterized by UV–Vis spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) and transmission electron microscopy (TEM) analyses. XRD and TEM results confirmed the formation of SeNPs in sizes ranging from 35 to 40 nm, with face-centered cubic (FCC) structures. The bioreduction process and validation of the formation of SeNPs was further confirmed by FTIR studies. The reduction in the biosynthesis of SeNPs using bacterial metabolite due to heavy metal cytotoxicity was analyzed by the colorimetric bioassay (SE Assay). The inhibition of selenite reduction and loss of red color in the presence of heavy metals may serve as a biosensor for heavy metal toxicity analysis. Thus, this biosensor development is aimed at improving the sensitivity and specificity of analytic detection.

ACS Style

Faheem Ahmed; Sourabh Dwivedi; Nagih Shaalan; Shalendra Kumar; Nishat Arshi; Adil Alshoaibi; Fohad Husain. Development of Selenium Nanoparticle Based Agriculture Sensor for Heavy Metal Toxicity Detection. Agriculture 2020, 10, 610 .

AMA Style

Faheem Ahmed, Sourabh Dwivedi, Nagih Shaalan, Shalendra Kumar, Nishat Arshi, Adil Alshoaibi, Fohad Husain. Development of Selenium Nanoparticle Based Agriculture Sensor for Heavy Metal Toxicity Detection. Agriculture. 2020; 10 (12):610.

Chicago/Turabian Style

Faheem Ahmed; Sourabh Dwivedi; Nagih Shaalan; Shalendra Kumar; Nishat Arshi; Adil Alshoaibi; Fohad Husain. 2020. "Development of Selenium Nanoparticle Based Agriculture Sensor for Heavy Metal Toxicity Detection." Agriculture 10, no. 12: 610.

Journal article
Published: 05 December 2020 in Coatings
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The unabated abuse of antibiotics has created a selection pressure that has resulted in the development of antimicrobial resistance (AMR) among pathogenic bacteria. AMR has become a global health concern in recent times and is responsible for a high number of mortalities occurring across the globe. Owing to the slow development of antibiotics, new chemotherapeutic antimicrobials with a novel mode of action is required urgently. Therefore, in the current investigation, we green synthesized a nanocomposite comprising zinc oxide nanoparticles functionalized with extracellular polysaccharide xanthan gum ([email protected]). Synthesized nanomaterial was characterized by structurally and morphologically using UV-visible spectroscopy, XRD, FTIR, BET, SEM and TEM. Subinhibitory concentrations of [email protected] were used to determine quorum sensing inhibitory activity against Gram-negative pathogens, Chromobacterium violaceum, and Serratia marcescens. [email protected] reduced quorum sensing (QS) regulated virulence factors such as violacein (61%), chitinase (70%) in C. violaceum and prodigiosin (71%) and protease (72%) in S. marcescens at 128 µg/mL concentration. Significant (p ≤ 0.05) inhibition of biofilm formation as well as preformed mature biofilms was also recorded along with the impaired production of EPS, swarming motility and cell surface hydrophobicity in both the test pathogens. The findings of this study clearly highlight the potency of [email protected] against the QS controlled virulence factors of drug-resistant pathogens that may be developed as effective inhibitors of QS and biofilms to mitigate the threat of multidrug resistance (MDR). [email protected] may be used alone or in combination with antimicrobial drugs against MDR bacterial pathogens. Further, it can be utilized in the food industry to counter the menace of contamination and spoilage caused by the formation of biofilms.

ACS Style

Fohad Mabood Husain; Imran Hasan; Faizan Abul Qais; Rais Ahmad Khan; Pravej Alam; Ali Alsalme. Fabrication of Zinc Oxide-Xanthan Gum Nanocomposite via Green Route: Attenuation of Quorum Sensing Regulated Virulence Functions and Mitigation of Biofilm in Gram-Negative Bacterial Pathogens. Coatings 2020, 10, 1190 .

AMA Style

Fohad Mabood Husain, Imran Hasan, Faizan Abul Qais, Rais Ahmad Khan, Pravej Alam, Ali Alsalme. Fabrication of Zinc Oxide-Xanthan Gum Nanocomposite via Green Route: Attenuation of Quorum Sensing Regulated Virulence Functions and Mitigation of Biofilm in Gram-Negative Bacterial Pathogens. Coatings. 2020; 10 (12):1190.

Chicago/Turabian Style

Fohad Mabood Husain; Imran Hasan; Faizan Abul Qais; Rais Ahmad Khan; Pravej Alam; Ali Alsalme. 2020. "Fabrication of Zinc Oxide-Xanthan Gum Nanocomposite via Green Route: Attenuation of Quorum Sensing Regulated Virulence Functions and Mitigation of Biofilm in Gram-Negative Bacterial Pathogens." Coatings 10, no. 12: 1190.

Journal article
Published: 29 November 2020 in Coatings
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Herein, a new and simple biogenic method for the preparation of gold nanoparticles (AuNPs) and their reduced graphene oxide based nanocomposites (Au-RGO) by using microwave irradiation method for antimicrobial and biofilm inhibition against foodborne pathogenic bacteria was reported. X-ray diffraction (XRD), Raman, and transmission electron microscopy (TEM) analyses confirmed that the AuNPs with face centered cubic (FCC) structure were indeed anchored onto the RGO sheets. Ultraviolet-Vis (UV-VIS) spectrum showed a shifting and broadening of absorption peaks of AuNPs when attached on the surface of RGO sheets. The effect of sub-inhibitory concentrations of Au-RGO nanocomposites on biofilm formation in five foodborne pathogens was assessed. Au-RGO nanocomposites reduced the formation of biofilm by 75%, 78%, 68%, 80% and 79% in L. monocytogenes, MRSA, E. coli, S. marcescens and P. aeruginosa, respectively. Exopolysaccharides (EPS), a vital component of the biofilm was also inhibited significantly and pre-formed mature biofilms were also reduced considerably. Further, this study demonstrated that the reactive oxygen species (ROS) generation induced in bacterial cells as a result of Au-RGO treatment could be the plausible mechanism for biofilm inhibitory action. The tested concentrations were found non-toxic to human embryonic kidney cell lines (HEK-293). The investigation highlights the broad-spectrum biofilm inhibitory properties of Au-RGO nanocomposites that could be exploited in the food industry to prevent biofilm-based food contamination.

ACS Style

Abdullah Aljaafari; Faheem Ahmed; Fohad Husain. Bio-Inspired Facile Synthesis of Graphene-Based Nanocomposites: Elucidation of Antimicrobial and Biofilm Inhibitory Potential against Foodborne Pathogenic Bacteria. Coatings 2020, 10, 1171 .

AMA Style

Abdullah Aljaafari, Faheem Ahmed, Fohad Husain. Bio-Inspired Facile Synthesis of Graphene-Based Nanocomposites: Elucidation of Antimicrobial and Biofilm Inhibitory Potential against Foodborne Pathogenic Bacteria. Coatings. 2020; 10 (12):1171.

Chicago/Turabian Style

Abdullah Aljaafari; Faheem Ahmed; Fohad Husain. 2020. "Bio-Inspired Facile Synthesis of Graphene-Based Nanocomposites: Elucidation of Antimicrobial and Biofilm Inhibitory Potential against Foodborne Pathogenic Bacteria." Coatings 10, no. 12: 1171.

Journal article
Published: 17 November 2020 in Polymers
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Ethylenediamine modified Ammonium polyphosphate (EDA-MAPP), and Charring-Foaming Agents (CFA) was prepared via a simple chemical approach and further utilizes for the preparation of Epoxy resin based intumescent flame retardation coatings. The ratio belongs to MAPP and CFA was fixed at 2:1 ratio. Comparative thermo gravimetric analysis TGA study of Modified Ammonium polyphosphate (MAPP) and Ammonium polyphosphate (APP) investigated. Sb2O3 was introduced into flame retardation coating formulation at various amounts to evaluate the synergistic action of Sb2O3 along with flame retardant coating system. The synergistic action of Sb2O3 on flame retardation coating formulation was studied by vertical burning test (UL-94V), thermo gravimetric analysis (TGA), Limited Oxygen Index (LOI), and Fourier Transform Infra-Red spectroscopy (FTIR). The UL-94V results indicated that adding Sb2O3 effectively increased flame retardancy and meets V-0 ratings at each concentration. The TGA results revealed that the amalgamation of Sb2O3 at each concentration effectively increased the thermal stability of the flame retardant coating system. Cone-calorimeter study results that Sb2O3 successfully minimized the combustion parameters like, Peak Heat Release Rate (PHRR), and Total Heat Release (THR). The FTIR result shows that Sb2O3 can react with MAPP and generates the dense-charred layer which prevents the transfer of heat and oxygen.

ACS Style

Riyazuddin Riyazuddin; Samrin Bano; Fohad Mabood Husain; Rais Ahmad Khan; Ali Alsalme; Jamal Akhter Siddique. Influence of Antimony Oxide on Epoxy Based Intumescent Flame Retardation Coating System. Polymers 2020, 12, 2721 .

AMA Style

Riyazuddin Riyazuddin, Samrin Bano, Fohad Mabood Husain, Rais Ahmad Khan, Ali Alsalme, Jamal Akhter Siddique. Influence of Antimony Oxide on Epoxy Based Intumescent Flame Retardation Coating System. Polymers. 2020; 12 (11):2721.

Chicago/Turabian Style

Riyazuddin Riyazuddin; Samrin Bano; Fohad Mabood Husain; Rais Ahmad Khan; Ali Alsalme; Jamal Akhter Siddique. 2020. "Influence of Antimony Oxide on Epoxy Based Intumescent Flame Retardation Coating System." Polymers 12, no. 11: 2721.

Journal article
Published: 09 November 2020 in Current Medicinal Chemistry
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: Over the past several decades, plant-derived products (phytochemicals) have been suggested to possess immense therapeutic potential. Among these phytochemicals, different flavonoids have been reported for their potent anticancer activity. To exhibit their anticancer potential, these flavonoids modulate different signaling pathways. Among these pathways, the mammalian target of rapamycin (mTOR) and associated phosphatidyl-inositiol 3-kinase (PI3K)/protein kinase B (Akt) signaling cascade has been suggested as a pivotal modulator of cell survival, proliferation, and death/apoptosis. Hence, targeting this cascade could be an ideal strategy to alleviate apoptosis and inhibit proliferation in different forms of cancer. The targeting of PI3K/Akt/mTOR by flavonoids have been well documented in the scientific literature. In the current communication, we have covered the anticancer potential of various flavonoids especially flavones, flavanols, and isoflavones that include apigenin, luteolin, biacalein, tangeretin, epigallocatechin-3-gallate, genistein, and diadzein especially dealing with mTOR targeting.

ACS Style

Badar Ul Islam; Mohd Shahnawaz Khan; Fohad Mabood Husain; Tabish Rehman; Torki A. Alzughaibi; Adel M. Abuzenadah; Maryam Urooj; Mohammad Amjad Kamal; Shams Tabrez. mTor Targeting by Different Flavonoids for Cancer Prevention. Current Medicinal Chemistry 2020, 27, 1 -12.

AMA Style

Badar Ul Islam, Mohd Shahnawaz Khan, Fohad Mabood Husain, Tabish Rehman, Torki A. Alzughaibi, Adel M. Abuzenadah, Maryam Urooj, Mohammad Amjad Kamal, Shams Tabrez. mTor Targeting by Different Flavonoids for Cancer Prevention. Current Medicinal Chemistry. 2020; 27 ():1-12.

Chicago/Turabian Style

Badar Ul Islam; Mohd Shahnawaz Khan; Fohad Mabood Husain; Tabish Rehman; Torki A. Alzughaibi; Adel M. Abuzenadah; Maryam Urooj; Mohammad Amjad Kamal; Shams Tabrez. 2020. "mTor Targeting by Different Flavonoids for Cancer Prevention." Current Medicinal Chemistry 27, no. : 1-12.

Chapter
Published: 06 October 2020 in Diabetic Foot Ulcer
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Diabetes and its related complications are considered as one of the major causes of morbidity and mortality round the globe [1]. Diabetes is a noncommunicable metabolic syndrome may either characterized by defective or decreased level of insulin secretion [2]. The current estimate on global diabetes epidemiology reveals that roughly under half of billion population is affected by diabetes and associated pathologies and is projected to increase by around 50% by 2045 [3].

ACS Style

Fohad Mabood Husain; Mohammad Shavez Khan; Mohammad Zubair; Altaf Khan; Saba Noor; Iftekhar Hassan; Mohammed Arshad; Mohd Shahnawaz Khan; Faizan Abul Qais; Salman Al-Amery. MRSA, EBSL, and Biofilm Formation in Diabetic Foot Ulcer Infections. Diabetic Foot Ulcer 2020, 149 -160.

AMA Style

Fohad Mabood Husain, Mohammad Shavez Khan, Mohammad Zubair, Altaf Khan, Saba Noor, Iftekhar Hassan, Mohammed Arshad, Mohd Shahnawaz Khan, Faizan Abul Qais, Salman Al-Amery. MRSA, EBSL, and Biofilm Formation in Diabetic Foot Ulcer Infections. Diabetic Foot Ulcer. 2020; ():149-160.

Chicago/Turabian Style

Fohad Mabood Husain; Mohammad Shavez Khan; Mohammad Zubair; Altaf Khan; Saba Noor; Iftekhar Hassan; Mohammed Arshad; Mohd Shahnawaz Khan; Faizan Abul Qais; Salman Al-Amery. 2020. "MRSA, EBSL, and Biofilm Formation in Diabetic Foot Ulcer Infections." Diabetic Foot Ulcer , no. : 149-160.

Journal article
Published: 30 September 2020 in Industrial Crops and Products
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Anticorrosive and antibacterial polyesteramide nanocomposite coating material was prepared from naturally available ecofriendly raw materials: Corn oil [CO] and citric acid [CA]. Corn oil was first derivatized into fatty amide diol (HECA) by base catalyzed amidation reaction. HECA was then treated with CA at desired temperature, forming CO based polyesteramide [CPEA], without any organic solvent, subsequently followed by addition of silver nitrate resulting in in situ formation of silver nanoparticles in CPEA matrix, producing Corn polyesteramide/silver nanocomposite [NCPEA]. The formation of NCPEA was supported by FTIR, UV, XRD, TEM and SEM analysis. NCPEA were applied on mild steel panels to prepare their coatings. NCPEA coatings were subjected to physico-mechanical and corrosion tests in different media. It was found that the coatings showed good scratch hardness, impact resistance, bending ability, and also performed well against various corrosive media (3.5 wt% HCl, 3.5 wt% NaCl and tap water) as investigated by Tafel analysis. Thermal analysis showed that the coatings can be safely employed upto 225 °C. Overall strategy is simple, using natural raw materials and employing solvent free synthesis.

ACS Style

Manawwer Alam; Naser M. Alandis; Naushad Ahmad; Fohad Mabood Husain. Anticorrosive and antibacterial nanocomposite coating material from sustainable resource. Industrial Crops and Products 2020, 158, 112955 .

AMA Style

Manawwer Alam, Naser M. Alandis, Naushad Ahmad, Fohad Mabood Husain. Anticorrosive and antibacterial nanocomposite coating material from sustainable resource. Industrial Crops and Products. 2020; 158 ():112955.

Chicago/Turabian Style

Manawwer Alam; Naser M. Alandis; Naushad Ahmad; Fohad Mabood Husain. 2020. "Anticorrosive and antibacterial nanocomposite coating material from sustainable resource." Industrial Crops and Products 158, no. : 112955.

Chapter
Published: 30 August 2020 in Functional Food Products and Sustainable Health
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Probiotics are live microorganisms or bacterial cultures that can have beneficial effects for the host when ingested. Probiotics must survive stressful conditions of the gastrointestinal tract by tolerating acid, bile, and gastric enzymes and must adhere to intestinal epithelial cells to colonize the gut. Moreover, probiotics should have antimicrobial effects against pathogenic microorganisms and desirable antibiotic susceptibility patterns. Many gastrointestinal diseases, such as diarrhea, irritable bowel syndrome, and chronic inflammatory bowel disease are caused by intestinal microflora imbalance, which is an important factor in bacterial translocation and infection. The current treatment of intestinal microbiota imbalance is using antibiotics; however, misuse or overuse of antibiotics contributes to resistance, which is one of the major public health problems worldwide. Another concern is the decreasing efficacy of antibiotics in treating human and animal infections because of the biofilm formation of pathogenic bacteria. Bacterial cells in biofilms are highly protected, less subjected to mutation, represent low metabolic activity, and become resistant to antibiotics. Therefore, the probiotic strains having both antimicrobial and antibiofilm properties may be expected to be therapeutically more effective. The antimicrobial probiotics having biofilm-dispersive properties can yield better clinical benefits as a therapeutic agent. Keeping in focus the promise shown by probiotics, current review is aimed to highlight the antibiofilm potential of probiotic microorganisms against drug-resistant pathogens.

ACS Style

Fohad Mabood Husain; Nasser A. Abdulatif Al-Shabib; Abdullah Alyousef; Altaf Khan; Mohammed Arshad; Iftekhar Hassan; Thamer A. AlBalawi; Iqbal Ahmad. Probiotic Bacteria Used in Food: A Novel Class of Antibiofilm Agent. Functional Food Products and Sustainable Health 2020, 25 -35.

AMA Style

Fohad Mabood Husain, Nasser A. Abdulatif Al-Shabib, Abdullah Alyousef, Altaf Khan, Mohammed Arshad, Iftekhar Hassan, Thamer A. AlBalawi, Iqbal Ahmad. Probiotic Bacteria Used in Food: A Novel Class of Antibiofilm Agent. Functional Food Products and Sustainable Health. 2020; ():25-35.

Chicago/Turabian Style

Fohad Mabood Husain; Nasser A. Abdulatif Al-Shabib; Abdullah Alyousef; Altaf Khan; Mohammed Arshad; Iftekhar Hassan; Thamer A. AlBalawi; Iqbal Ahmad. 2020. "Probiotic Bacteria Used in Food: A Novel Class of Antibiofilm Agent." Functional Food Products and Sustainable Health , no. : 25-35.

Paper
Published: 17 August 2020 in RSC Advances
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Novel, safe, and effective antilisterial agents are required in order to prevent Listeria monocytogenes infections and maintain food safety.

ACS Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Mohammad Nadeem; Mohd Shahnawaz Khan; Fahad Al-Qurainy; Abdullah A. Alyousef; Mohammed Arshad; Altaf Khan; Javed Masood Khan; Pravej Alam; Thamer AlBalawi; Syed Ali Shahzad. Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability. RSC Advances 2020, 10, 30139 -30149.

AMA Style

Nasser A. Al-Shabib, Fohad Mabood Husain, Mohammad Nadeem, Mohd Shahnawaz Khan, Fahad Al-Qurainy, Abdullah A. Alyousef, Mohammed Arshad, Altaf Khan, Javed Masood Khan, Pravej Alam, Thamer AlBalawi, Syed Ali Shahzad. Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability. RSC Advances. 2020; 10 (50):30139-30149.

Chicago/Turabian Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Mohammad Nadeem; Mohd Shahnawaz Khan; Fahad Al-Qurainy; Abdullah A. Alyousef; Mohammed Arshad; Altaf Khan; Javed Masood Khan; Pravej Alam; Thamer AlBalawi; Syed Ali Shahzad. 2020. "Bio-inspired facile fabrication of silver nanoparticles from in vitro grown shoots of Tamarix nilotica: explication of its potential in impeding growth and biofilms of Listeria monocytogenes and assessment of wound healing ability." RSC Advances 10, no. 50: 30139-30149.

Original research article
Published: 28 July 2020 in Frontiers in Microbiology
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There is grave necessity to counter the menace of drug-resistant biofilms of pathogens using nanomaterials. Moreover, we need to produce nanoparticles (NPs) using inexpensive clean biological approaches that demonstrate broad-spectrum inhibition of microbial biofilms and cytotoxicity against HepG2 cell lines. In the current research work, titanium dioxide (TiO2) NPs were fabricated through an environmentally friendly green process using the root extract of Withania somnifera as the stabilizing and reducing agent to examine its antibiofilm and anticancer potential. Further, X-ray diffraction (XRD), Fourier transform infrared (FTIR), scanning electron microscopy (SEM), transmission electron micrograph (TEM), energy-dispersive X-ray spectroscopy (EDS), dynamic light scattering (DLS), thermogravimetric analysis (TGA), and Brunauer-Emmett-Teller (BET) techniques were used for determining the crystallinity, functional groups involved, shape, size, thermal behavior, surface area, and porosity measurement, respectively, of the synthesized TiO2 NPs. Antimicrobial potential of the TiO2 NPs was determined by evaluating the minimum inhibitory concentration (MIC) against Escherichia coli, Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus, Listeria monocytogenes, Serratia marcescens, and Candida albicans. Furthermore, at levels below the MIC (0.5 × MIC), TiO2 NPs demonstrated significant inhibition of biofilm formation (43–71%) and mature biofilms (24–64%) in all test pathogens. Cell death due to enhanced reactive oxygen species (ROS) production could be responsible for the impaired biofilm production in TiO2 NP–treated pathogens. The synthesized NPs induced considerable reduction in the viability of HepG2 in vitro and could prove effective in controlling liver cancer. In summary, the green synthesized TiO2 NPs demonstrate multifarious biological properties and could be used as an anti-infective agent to treat biofilm-based infections and cancer.

ACS Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Faizan Abul Qais; Naushad Ahmad; Altaf Khan; Abdullah A. Alyousef; Mohammed Arshad; Saba Noor; Javed Masood Khan; Pravej Alam; Thamer H. AlBalawi; Syed Ali Shahzad. Phyto-Mediated Synthesis of Porous Titanium Dioxide Nanoparticles From Withania somnifera Root Extract: Broad-Spectrum Attenuation of Biofilm and Cytotoxic Properties Against HepG2 Cell Lines. Frontiers in Microbiology 2020, 11, 1680 .

AMA Style

Nasser A. Al-Shabib, Fohad Mabood Husain, Faizan Abul Qais, Naushad Ahmad, Altaf Khan, Abdullah A. Alyousef, Mohammed Arshad, Saba Noor, Javed Masood Khan, Pravej Alam, Thamer H. AlBalawi, Syed Ali Shahzad. Phyto-Mediated Synthesis of Porous Titanium Dioxide Nanoparticles From Withania somnifera Root Extract: Broad-Spectrum Attenuation of Biofilm and Cytotoxic Properties Against HepG2 Cell Lines. Frontiers in Microbiology. 2020; 11 ():1680.

Chicago/Turabian Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Faizan Abul Qais; Naushad Ahmad; Altaf Khan; Abdullah A. Alyousef; Mohammed Arshad; Saba Noor; Javed Masood Khan; Pravej Alam; Thamer H. AlBalawi; Syed Ali Shahzad. 2020. "Phyto-Mediated Synthesis of Porous Titanium Dioxide Nanoparticles From Withania somnifera Root Extract: Broad-Spectrum Attenuation of Biofilm and Cytotoxic Properties Against HepG2 Cell Lines." Frontiers in Microbiology 11, no. : 1680.

Journal article
Published: 17 July 2020 in Journal of King Saud University - Science
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It is important to understand the nature and mechanism of interaction of drugs with serum albumins as such interactions govern their pharmacokinetics and pharmacodynamics. Plumbagin is a natural phytocompound, mainly present in the bark of Plumbaginaceae family plants and it has numerous therapeutic potentials. In this study, the interaction of plumbagin with human serum albumin (HSA) was deciphered using an array of biophysical and computational tools. The UV-vis spectroscopy established the formation of plumbagin-HSA complex with binding constant as 2.32 × 104 M-1. Fluorescence revealed found that there was static mode of quenching of HSA’s fluorescence by plumbagin. The binding constant obtained by fluorescence spectroscopy was of the similar order as in case of UV-vis spectroscopy. The negative value of ΔH° (-3.67) indicated an endothermic nature of the reaction and negative value of ΔG° (-6.40 to -6.58) confirmed that complexation of plumbagin to HSA was spontaneous. There was nearly one binding site in HSA for plumbagin. Titration of plumbagin to HSA in presence of site-specific markers showed that plumbagin interacted at sub-domain IIA of HSA, commonly known as Sudlow’s site I. Moreover, the binding resulted in changes in microenvironment of tryptophan while of tyrosine residues remained approximately unchanged. This interaction also resulted in decrease of α-helical content of the studied serum protein. FRET calculations revealed that distance between plumbagin and HSA’s fluorophore (Trp214) was 2.27 nm. Finally, molecular docking studies substantiated the in vitro finding. Plumbagin formed hydrogen bond with Lys199 and Arg257 of HSA. Additionally, Arg222, His242, and Ala261 interacted via van der Waals forces and Leu238, Leu260, Ile264, Ile290, and Ala291 of HSA interacted with plumbagin through hydrophobic forces.

ACS Style

Faizan Abul Qais; Fohad Mabood Husain; Rais Ahmad Khan; Iqbal Ahmad; Iftekhar Hassan. Deciphering the interaction of plumbagin with human serum albumin: A combined biophysical and molecular docking study. Journal of King Saud University - Science 2020, 32, 2854 -2862.

AMA Style

Faizan Abul Qais, Fohad Mabood Husain, Rais Ahmad Khan, Iqbal Ahmad, Iftekhar Hassan. Deciphering the interaction of plumbagin with human serum albumin: A combined biophysical and molecular docking study. Journal of King Saud University - Science. 2020; 32 (6):2854-2862.

Chicago/Turabian Style

Faizan Abul Qais; Fohad Mabood Husain; Rais Ahmad Khan; Iqbal Ahmad; Iftekhar Hassan. 2020. "Deciphering the interaction of plumbagin with human serum albumin: A combined biophysical and molecular docking study." Journal of King Saud University - Science 32, no. 6: 2854-2862.

Journal article
Published: 16 January 2020 in Saudi Journal of Biological Sciences
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Quorum sensing (QS) plays a crucial role in different stages of biofilm development, virulence production, and subsequently to the growth of bacteria in food environments. Biofilm mediated spoilage of food is one of the ongoing challenge faced by the food industry worldwide as it incurs substantial economic losses and leads to various health issues. In the present investigation, we studied the interference of quorum sensing, its regulated virulence functions, and biofilm in food-associated bacteria by colorant azorubine. In vitro bioassays demonstrated significant inhibition of QS and its coordinated virulence functions in Chromobacterium violaceum 12472 (violacein) and Pseudomonas aeruginosa PAO1 (elastase, protease, pyocyanin, and alginate). Further, the decrease in the production EPS (49–63%) and swarming motility (61–83%) of the pathogens was also recorded at sub-MICs. Azorubine demonstrated broad-spectrum biofilm inhibitory potency (50–65%) against Chromobacterium violaceum, Pseudomonas aeruginosa, E. coli O157:H7, Serratia marcescens, and Listeria monocytogenes. ROS generation due to the interaction between bacteria and azorubine could be responsible for the biofilm inhibitory action of the food colorant. Findings of the in vitro studies were well supported by molecular docking and simulation analysis of azorubine and QS virulence proteins. Azorubine showed strong binding to PqsA as compared to other virulent proteins (LasR, Vfr, and QscR). Thus, it is concluded that azorubine is a promising candidate to ensure food safety by curbing the menace of bacterial QS and biofilm-based spoilage of food and reduce economic losses.

ACS Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Tabish Rehman; Abdullah A. Alyousef; Mohammed Arshad; Altaf Khan; Javed Masood Khan; Pravej Alam; Thamer A. AlBalawi; Syed Ali Shahzad; Jakeera B. Syed; Mohamed F. Al-Ajmi. Food color ‘Azorubine’ interferes with quorum sensing regulated functions and obliterates biofilm formed by food associated bacteria: An in vitro and in silico approach. Saudi Journal of Biological Sciences 2020, 27, 1080 -1090.

AMA Style

Nasser A. Al-Shabib, Fohad Mabood Husain, Tabish Rehman, Abdullah A. Alyousef, Mohammed Arshad, Altaf Khan, Javed Masood Khan, Pravej Alam, Thamer A. AlBalawi, Syed Ali Shahzad, Jakeera B. Syed, Mohamed F. Al-Ajmi. Food color ‘Azorubine’ interferes with quorum sensing regulated functions and obliterates biofilm formed by food associated bacteria: An in vitro and in silico approach. Saudi Journal of Biological Sciences. 2020; 27 (4):1080-1090.

Chicago/Turabian Style

Nasser A. Al-Shabib; Fohad Mabood Husain; Tabish Rehman; Abdullah A. Alyousef; Mohammed Arshad; Altaf Khan; Javed Masood Khan; Pravej Alam; Thamer A. AlBalawi; Syed Ali Shahzad; Jakeera B. Syed; Mohamed F. Al-Ajmi. 2020. "Food color ‘Azorubine’ interferes with quorum sensing regulated functions and obliterates biofilm formed by food associated bacteria: An in vitro and in silico approach." Saudi Journal of Biological Sciences 27, no. 4: 1080-1090.