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To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes of these CuNPs were in the range of 150–200 nm. EDAX analysis confirmed the presence of copper and oxygen and revealed that copper existed in an oxidized form. FTIR spectroscopy showed the presence of different functional groups in these synthesized nanoparticles. GC-MS analysis revealed compounds such as Anethole D-limonene, heptadecanoic acid, 16-methyl-, methyl ester, myristene, methyl eugenol, and methyl stearate, indicating the presence of functional groups. The antibacterial activities of the three extracts from spices were analyzed using growth zone inhibition and TLC-bioautography methods. The results showed that star anise spice extract had the highest antibacterial activity. These results indicate that such CuNPs phyto-formulated with spice extracts having antibacterial properties could be used as potential therapeutics for microbial diseases.
Gayathri Vijayakumar; Hindhuja Kesavan; Anisha Kannan; Dhanalakshmi Arulanandam; Jeong Kim; Kwang Kim; Hak Song; Hyung Kim; Senthil Rangarajulu. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes 2021, 9, 1341 .
AMA StyleGayathri Vijayakumar, Hindhuja Kesavan, Anisha Kannan, Dhanalakshmi Arulanandam, Jeong Kim, Kwang Kim, Hak Song, Hyung Kim, Senthil Rangarajulu. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes. 2021; 9 (8):1341.
Chicago/Turabian StyleGayathri Vijayakumar; Hindhuja Kesavan; Anisha Kannan; Dhanalakshmi Arulanandam; Jeong Kim; Kwang Kim; Hak Song; Hyung Kim; Senthil Rangarajulu. 2021. "Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties." Processes 9, no. 8: 1341.
The utilization of biological resources on the manufacture of nano silver has attracted the interest of researchers to develop an eco-friendly, cost-effective technology in nanomaterials production. In the present study, plant-mediated silver nanoparticles (AgNPs) were synthesized using aqueous leaf extracts of the Copperpod plant, which was well characterized. The ultraviolet-visible spectrophotometric study showed a maximum absorbance peak at 425 nm, and the observation of transmission electron microscopic features revealed that the nanoparticles size ranged between 20 and 70 nm. The synthesized AgNPs were tested for in vitro cytotoxic effects against cancerous cells, such as HepG2, A549 and MCF-7 cells. The findings showed that the IC50 values of AgNPs against cancerous cells viz., HepG2, MCF-7 and A549 cells, were observed to be 69 µg/mL, 62 µg/mL and 53 µg/mL, respectively. In addition, the apoptosis property was analysed using propidium iodide and acridine orange-ethidium bromide via the DNA fragmentation technique. Thus, the outcomes of the current analysis presume that the plant mediated AgNPs obtained from a synthesized Copperpod plant possess significant anti-cancer properties against various cancerous cells.
Balashanmugam Pannerselvam; Devasena Thiyagarajan; Annamalai Pazhani; Kalaichelvan Thangavelu; Hyung Kim; Senthil Rangarajulu. Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines. Processes 2021, 9, 888 .
AMA StyleBalashanmugam Pannerselvam, Devasena Thiyagarajan, Annamalai Pazhani, Kalaichelvan Thangavelu, Hyung Kim, Senthil Rangarajulu. Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines. Processes. 2021; 9 (5):888.
Chicago/Turabian StyleBalashanmugam Pannerselvam; Devasena Thiyagarajan; Annamalai Pazhani; Kalaichelvan Thangavelu; Hyung Kim; Senthil Rangarajulu. 2021. "Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines." Processes 9, no. 5: 888.
Cancer is one of the most dangerous threats to human health and possibly the utmost task for current medicine. Currently, bio-based synthesis of nanoparticles from plants has gained much interest due to its potential medicinal applications. In the present study, a biological approach was employed for biogenic (green) synthesis of silver nanoparticles (AgNPs) using dried leaf extract of Asian spider flower (Asf). The biogenic synthesis of Asf-AgNPs (Asian spider flower-Silver nanoparticles) was established using ultra violet-visible (UV-vis) spectra which exhibited a wide superficial plasmon resonance of AgNPs at 445 nm. These nanoparticles clearly showed the formation of poly-disperse crystalline solids (spherical shape) with particle size range of
Balashanmugam Pannerselvam; Prabhu Durai; Devasena Thiyagarajan; Hak Jin Song; Kwang Jin Kim; Yun Seok Jung; Hyung Joo Kim; Senthil Kumaran Rangarajulu. Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells. Processes 2020, 8, 430 .
AMA StyleBalashanmugam Pannerselvam, Prabhu Durai, Devasena Thiyagarajan, Hak Jin Song, Kwang Jin Kim, Yun Seok Jung, Hyung Joo Kim, Senthil Kumaran Rangarajulu. Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells. Processes. 2020; 8 (4):430.
Chicago/Turabian StyleBalashanmugam Pannerselvam; Prabhu Durai; Devasena Thiyagarajan; Hak Jin Song; Kwang Jin Kim; Yun Seok Jung; Hyung Joo Kim; Senthil Kumaran Rangarajulu. 2020. "Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells." Processes 8, no. 4: 430.
Among the metal–oxide nanoparticles, pure and doped zinc oxide (ZnO) has been widely studied due to their potential applications, such as electrical, thermal and optical materials. In this context, samples of undoped ZnO and Cr-doped ZnO powders were prepared using a solution-combustion route. The formation of the combustion products was confirmed by X-ray diffraction analysis. The samples were well characterized using the Fourier transform infrared spectroscopy and scanning electron microscopy techniques. In the sample, photoluminescence studies showed that ZnO could be excited at 267 nm, while an ultraviolet emission centred at \({\sim }383\,\hbox {nm}\) was exhibited. Antibacterial-activity testing was carried out using the disc-diffusion method.
Yong-Keun Choi; Kyeong Min Kim; Yongcheol Jang; Hakyung Jeong; Vijay Singh; Senthil Kumaran Rangarajulu. Investigations on the ZnO- and Cr-doped ZnO powders. Bulletin of Materials Science 2019, 42, 150 .
AMA StyleYong-Keun Choi, Kyeong Min Kim, Yongcheol Jang, Hakyung Jeong, Vijay Singh, Senthil Kumaran Rangarajulu. Investigations on the ZnO- and Cr-doped ZnO powders. Bulletin of Materials Science. 2019; 42 (4):150.
Chicago/Turabian StyleYong-Keun Choi; Kyeong Min Kim; Yongcheol Jang; Hakyung Jeong; Vijay Singh; Senthil Kumaran Rangarajulu. 2019. "Investigations on the ZnO- and Cr-doped ZnO powders." Bulletin of Materials Science 42, no. 4: 150.
A plant health monitoring method based on a plant microbial fuel cell (PMFC) technique was investigated. The reducing action of plant exudates on electrochemically active bacteria (EAB) in the rhizosphere was used as the basis of the method. Ardisia pusilla was used as an experimental plant. The results indicated that the open circuit potential (OCP) development from the PMFC is closely related to plant health and affected by the cultivation environment. Distinctive OCP developments were observed using the PMFC with live, dead (heat shocked), or no plant. Cultivation conditions such as watering, fertilizing, temperature variation, and lighting affected OCP development from the PMFC. Plant root viability analysis indicated that OCP development from the PMFC is closely related to plant health. These results suggest that this method based on the PMFC technique is a facile, cost‐effective, and rapid tool for monitoring plant health.
Tae Hang Yoon; Hak Jin Song; Woo Young Jung; Ji Eun Kim; Kwang Jin Kim; Ho Hyun Kim; Rangarajulu Senthil Kumaran; Sunghyun Kim; Hyung Joo Kim. Monitoring Plant Health Using a Plant Microbial Fuel Cell. Bulletin of the Korean Chemical Society 2018, 39, 1193 -1197.
AMA StyleTae Hang Yoon, Hak Jin Song, Woo Young Jung, Ji Eun Kim, Kwang Jin Kim, Ho Hyun Kim, Rangarajulu Senthil Kumaran, Sunghyun Kim, Hyung Joo Kim. Monitoring Plant Health Using a Plant Microbial Fuel Cell. Bulletin of the Korean Chemical Society. 2018; 39 (10):1193-1197.
Chicago/Turabian StyleTae Hang Yoon; Hak Jin Song; Woo Young Jung; Ji Eun Kim; Kwang Jin Kim; Ho Hyun Kim; Rangarajulu Senthil Kumaran; Sunghyun Kim; Hyung Joo Kim. 2018. "Monitoring Plant Health Using a Plant Microbial Fuel Cell." Bulletin of the Korean Chemical Society 39, no. 10: 1193-1197.
The synthesis of metallic nanoparticles using plant extracts has attracted much attention. In this study, silver nanoparticles (AgNPs) were synthesized using the Ginkgo biloba plant-leaf extract and their antibacterial and larvicidal activities were investigated. AgNPs were characterized using UV-visible, X-ray diffraction (XRD), Fourier transform infrared (FTIR), energy-dispersive X-ray (EDX) spectroscopy and field emission scanning electron microscope (FESEM) analysis. The UV-visible spectral analysis showed a surface plasmon resonance (SPR) peak at 430 nm, the FESEM analysis revealed size of AgNPs between 25–45 nm and the XRD data confirmed the formation of the AgNPs using G. biloba-mediated green synthesis. AgNPs showed the highest antibacterial activities against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtills, Enterobacter aerogens, Salmonella paratyphi and Escherichia coli. Also, the AgNPs exerted a significant larvicidal effect on the Culex mosquito larvae.
Pannerselvam Balashanmugam; Hyung Joo Kim; Vijay Singh; Rangarajulu Senthil Kumaran. Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects. Nanoscience and Nanotechnology Letters 2018, 10, 422 -428.
AMA StylePannerselvam Balashanmugam, Hyung Joo Kim, Vijay Singh, Rangarajulu Senthil Kumaran. Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects. Nanoscience and Nanotechnology Letters. 2018; 10 (3):422-428.
Chicago/Turabian StylePannerselvam Balashanmugam; Hyung Joo Kim; Vijay Singh; Rangarajulu Senthil Kumaran. 2018. "Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects." Nanoscience and Nanotechnology Letters 10, no. 3: 422-428.
S.U. Pawar; Rangarajulu Senthil Kumaran; M. Kumar; K.S. Yun; S.J. Dhoble; Vijay Singh. On the infrared emissions of Er3+/Yb3+ co-doped SrAl2O4. Optik 2017, 136, 581 -585.
AMA StyleS.U. Pawar, Rangarajulu Senthil Kumaran, M. Kumar, K.S. Yun, S.J. Dhoble, Vijay Singh. On the infrared emissions of Er3+/Yb3+ co-doped SrAl2O4. Optik. 2017; 136 ():581-585.
Chicago/Turabian StyleS.U. Pawar; Rangarajulu Senthil Kumaran; M. Kumar; K.S. Yun; S.J. Dhoble; Vijay Singh. 2017. "On the infrared emissions of Er3+/Yb3+ co-doped SrAl2O4." Optik 136, no. : 581-585.
Microbial algal system can serve as a potential source for the production of much high value bioproducts and biofuels. The quality and intensity of light are the key elements to optimize the production of algal biomass and fatty acid contents. This study presents the effect of differential LED flashing light conditions on the growth of microalgae, Acutodesmus obliquus. The induced light stress was optimized for its biomass and fatty acid content. The microalgae are exposed to various frequency of intermittent LED flashing light (blue and red lights) at three different phases in the 18 day cell growth (log, lag and stationary phase). The frequency of light flashing rate was adjusted to 120, 10, 5, 3.75, and 1 times per min. The effect of light stress on growth and fatty acids composition of A. obliquus induced an increase in algae growth and fatty acid production. Different optimal timing for light stress was subjected to elucidate the effect of light stress on algae growth and fatty acid production. The results showed an increase in the algae growth (1.2mg/L of chl a content) under light stress condition at FT10 (flashing time, 10 times per min) from the initial day (log phase) compared with the control experiment (0.4 mg/L of chl a content). However, the total fatty acids (71 mg/g) and volumetric FAME production (9.4 ml/l) level was found to be significant under FT5 (flashing time, 5 times per min), adopting flashing light from day 10 (stationary phase). TEM studies also revealed the deposition of lipid to be largest in the 18 day old cells under flashing light (FT5) condition, representing maximum accumulation of lipids bodies (up to 770 nm diameter in particle size) occupying approximately 42% of the total area of the cell.
Yong-Keun Choi; Rangarajulu Senthil Kumaran; Hyeon Jin Jeon; Hak-Jin Song; Yung-Hun Yang; Sang Hyun Lee; Kyung-Guen Song; Kwang Jin Kim; Vijay Singh; Hyung Joo Kim. LED light stress induced biomass and fatty acid production in microalgal biosystem, Acutodesmus obliquus. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 2015, 145, 245 -253.
AMA StyleYong-Keun Choi, Rangarajulu Senthil Kumaran, Hyeon Jin Jeon, Hak-Jin Song, Yung-Hun Yang, Sang Hyun Lee, Kyung-Guen Song, Kwang Jin Kim, Vijay Singh, Hyung Joo Kim. LED light stress induced biomass and fatty acid production in microalgal biosystem, Acutodesmus obliquus. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 2015; 145 ():245-253.
Chicago/Turabian StyleYong-Keun Choi; Rangarajulu Senthil Kumaran; Hyeon Jin Jeon; Hak-Jin Song; Yung-Hun Yang; Sang Hyun Lee; Kyung-Guen Song; Kwang Jin Kim; Vijay Singh; Hyung Joo Kim. 2015. "LED light stress induced biomass and fatty acid production in microalgal biosystem, Acutodesmus obliquus." Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 145, no. : 245-253.
Water-dispersible MgO nanoparticles were tested to investigate their cytotoxic effects on oxidative stress gene expression. In this in vitro study, genes related to reactive oxygen species (ROS), glutathione S-transferase (GST) and catalase, were quantified using real-time polymerase chain reactions (molecular level) and molecular beacon technologies (cellular level). The monodispersed MgO nanoparticles, 20 nm in size, were used to treat human cancer cell lines (liver cancer epithelial cells) at different concentrations (25, 75 and 150 µg/mL) and incubation times (24, 48 and 72 h). Both the genetic and cellular cytotoxic screening methods produced consistent results, showing that GST and catalase ROS gene expression was maximized at 150 µg/mL nanoparticle treatment with 48 h incubation. However, the genotoxic effect of MgO nanoparticles was not significant compared with control experiments, which indicates its significant potential applications in nanomedicine as a diagnostic and therapeutic tool.
Rangarajulu Senthil Kumaran; Yong-Keun Choi; Vijay Singh; Hak-Jin Song; Kyung-Guen Song; Kwang Jin Kim; Hyung Joo Kim. In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. International Journal of Molecular Sciences 2015, 16, 7551 -7564.
AMA StyleRangarajulu Senthil Kumaran, Yong-Keun Choi, Vijay Singh, Hak-Jin Song, Kyung-Guen Song, Kwang Jin Kim, Hyung Joo Kim. In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes. International Journal of Molecular Sciences. 2015; 16 (12):7551-7564.
Chicago/Turabian StyleRangarajulu Senthil Kumaran; Yong-Keun Choi; Vijay Singh; Hak-Jin Song; Kyung-Guen Song; Kwang Jin Kim; Hyung Joo Kim. 2015. "In Vitro Cytotoxic Evaluation of MgO Nanoparticles and Their Effect on the Expression of ROS Genes." International Journal of Molecular Sciences 16, no. 12: 7551-7564.
The objective of this study was to assess the cytotoxicity of water-dispersible CuO nanoparticles by quantifying the reactive oxygen species (ROS)-related genes (glutathione S-transferase (GST) and catalase) using real-time polymerase chain reaction (RT-PCR). Monodisperse CuO nanoparticles of 14 nm in size were used. Cytotoxicity of CuO nanoparticles was evaluated under in vitro condition at different concentrations (10, 50, and 100 μg/ml) and incubation times (12, 24, and 48 h) with human cancer cell lines (breast cancer epithelial cells). The genetic level cytotoxic screening produced consistent results showing that GST and catalase ROS gene expression was maximized in 24 h incubation at 100 μg/ml concentration of CuO nanoparticles. However, the cytotoxicity of water-dispersible CuO nanoparticle was not significant compared with control experiments, demonstrating its high potential in the application of nanomedicines for a diagnostic and therapeutic tool.
Rangarajulu Senthil Kumaran; Yong-Keun Choi; Hyung Joo Kim; Kwang Jin Kim. Quantitation of Oxidative Stress Gene Expression in MCF-7 Human Cell Lines Treated with Water-Dispersible CuO Nanoparticles. Applied Biochemistry and Biotechnology 2014, 173, 731 -740.
AMA StyleRangarajulu Senthil Kumaran, Yong-Keun Choi, Hyung Joo Kim, Kwang Jin Kim. Quantitation of Oxidative Stress Gene Expression in MCF-7 Human Cell Lines Treated with Water-Dispersible CuO Nanoparticles. Applied Biochemistry and Biotechnology. 2014; 173 (3):731-740.
Chicago/Turabian StyleRangarajulu Senthil Kumaran; Yong-Keun Choi; Hyung Joo Kim; Kwang Jin Kim. 2014. "Quantitation of Oxidative Stress Gene Expression in MCF-7 Human Cell Lines Treated with Water-Dispersible CuO Nanoparticles." Applied Biochemistry and Biotechnology 173, no. 3: 731-740.
Hyeon-Jin Jeon; Yong-Keun Choi; Rangarajulu Senthil Kumaran; Sung-Hyun Kim; Kyung-Guen Song; Seok-Won Hong; Mi-A Kim; Hyung-Joo Kim. Electrochemical Control of Methane Emission from Lake Sediment Using Microbial Fuel Cells. Bulletin of the Korean Chemical Society 2012, 33, 2401 -2404.
AMA StyleHyeon-Jin Jeon, Yong-Keun Choi, Rangarajulu Senthil Kumaran, Sung-Hyun Kim, Kyung-Guen Song, Seok-Won Hong, Mi-A Kim, Hyung-Joo Kim. Electrochemical Control of Methane Emission from Lake Sediment Using Microbial Fuel Cells. Bulletin of the Korean Chemical Society. 2012; 33 (7):2401-2404.
Chicago/Turabian StyleHyeon-Jin Jeon; Yong-Keun Choi; Rangarajulu Senthil Kumaran; Sung-Hyun Kim; Kyung-Guen Song; Seok-Won Hong; Mi-A Kim; Hyung-Joo Kim. 2012. "Electrochemical Control of Methane Emission from Lake Sediment Using Microbial Fuel Cells." Bulletin of the Korean Chemical Society 33, no. 7: 2401-2404.
Rangarajulu Senthil Kumaran; Hyung Joo Kim; Byung-Ki Hur. Taxol-producing fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering 2011, 111, 731 .
AMA StyleRangarajulu Senthil Kumaran, Hyung Joo Kim, Byung-Ki Hur. Taxol-producing fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering. 2011; 111 (6):731.
Chicago/Turabian StyleRangarajulu Senthil Kumaran; Hyung Joo Kim; Byung-Ki Hur. 2011. "Taxol-producing fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata." Journal of Bioscience and Bioengineering 111, no. 6: 731.
The fungus Colletotrichum capsici was isolated from the diseased fruits of Chilli plant, Capsicum annuum. The isolated test fungus was identified by its morphological and molecular characteristic features. For the first time, the fungus was screened for the production of taxol on modified liquid medium. The presence of taxol was confirmed by the spectroscopic and chromatographic methods of analyses. The amount of taxol produced by this fungus was quantified by HPLC. The maximum amount of fungal taxol production was recorded as 687 μg/L. The production rate was 13 740‐fold higher than that, previously reported for the fungus Taxomyces andreanae. The extracted fungal taxol showed a strong cytotoxic activity in an in vitro culture of human cancer cells indicating that the increase in taxol concentration induces increased cell death. A PCR‐based screening for taxadiene synthase (ts), a unique gene in the formation of the taxane skeleton, confirmed the molecular blueprint for taxol biosynthesis. The results show that the fungus C. capsici is an excellent candidate for an alternate source of taxol supply and can serve as a potential species for genetic engineering to enhance the production of taxol to a higher level.
Rangarajulu Senthil Kumaran; HeeHoon Jung; Hyung Joo Kim. In vitro screening of taxol, an anticancer drug produced by the fungus, Colletotrichum capsici. Engineering in Life Sciences 2011, 11, 264 -271.
AMA StyleRangarajulu Senthil Kumaran, HeeHoon Jung, Hyung Joo Kim. In vitro screening of taxol, an anticancer drug produced by the fungus, Colletotrichum capsici. Engineering in Life Sciences. 2011; 11 (3):264-271.
Chicago/Turabian StyleRangarajulu Senthil Kumaran; HeeHoon Jung; Hyung Joo Kim. 2011. "In vitro screening of taxol, an anticancer drug produced by the fungus, Colletotrichum capsici." Engineering in Life Sciences 11, no. 3: 264-271.
Bong-Geun Jeong; Seok-Won Hong; Yong-Su Choi; Rangarajulu Senthil Kumaran; Mi-A Kim; Sung-Hyun Kim; Hyung-Joo Kim. Isolation of Tributyltin Chloride Resistance Bacteria and Rapid Electrochemical Determination of Bacterial Organotin Degradation Activity. Bulletin of the Korean Chemical Society 2011, 32, 356 -358.
AMA StyleBong-Geun Jeong, Seok-Won Hong, Yong-Su Choi, Rangarajulu Senthil Kumaran, Mi-A Kim, Sung-Hyun Kim, Hyung-Joo Kim. Isolation of Tributyltin Chloride Resistance Bacteria and Rapid Electrochemical Determination of Bacterial Organotin Degradation Activity. Bulletin of the Korean Chemical Society. 2011; 32 (1):356-358.
Chicago/Turabian StyleBong-Geun Jeong; Seok-Won Hong; Yong-Su Choi; Rangarajulu Senthil Kumaran; Mi-A Kim; Sung-Hyun Kim; Hyung-Joo Kim. 2011. "Isolation of Tributyltin Chloride Resistance Bacteria and Rapid Electrochemical Determination of Bacterial Organotin Degradation Activity." Bulletin of the Korean Chemical Society 32, no. 1: 356-358.
The endophytic fungi, Pestalotiopsis versicolor and Pestalotiopsis neglecta, were isolated from the healthy leaves and bark of the Japanese Yew tree, Taxus cuspidata. The fungal species were identified by their characteristic culture morphology and molecular analysis. For the first time, the test fungi were screened for the production of taxol in modified liquid medium. The presence of taxol was confirmed by HPLC, (1)H NMR, and LC-MS methods of analysis. The maximum amount of taxol production in P. versicolor was recorded as 478 μg/l. The production rate was increased to 9560-fold than that found in the culture broth of earlier reported fungus, Taxomyces andreanae. The extracted fungal taxol showed a strong cytotoxic activity in the in vitro culture of tested human cancer cells by apoptotic assay indicating that the increase in taxol concentration induces increased cell death. A PCR-based screening for ts, a unique gene in the formation of the taxane skeleton, confirmed the molecular blueprint for taxol biosynthesis. The results designate that the fungal endophyte, P. versicolor, is an excellent candidate for an alternate source of taxol supply and can also serve as a potential species for genetic engineering to enhance the production of taxol to a higher level.
Rangarajulu Senthil Kumaran; Hyung Joo Kim; Byung-Ki Hur. Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering 2010, 110, 541 -546.
AMA StyleRangarajulu Senthil Kumaran, Hyung Joo Kim, Byung-Ki Hur. Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata. Journal of Bioscience and Bioengineering. 2010; 110 (5):541-546.
Chicago/Turabian StyleRangarajulu Senthil Kumaran; Hyung Joo Kim; Byung-Ki Hur. 2010. "Taxol promising fungal endophyte, Pestalotiopsis species isolated from Taxus cuspidata." Journal of Bioscience and Bioengineering 110, no. 5: 541-546.