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Examination of morphological characters of two arbuscular mycorrhizal fungi (AMF; phylum Glomeromycota), which produced clusters with glomoid spores in single-species cultures, suggested that they were undescribed species, one belonging to the genera Dominikia, Microdominikia or Kamienskia, and one to the genus Glomus. Phylogenetic analyses of the nuc rDNA small subunit (18S), internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) and large subunit (28S) (= 18S-ITS-28S) region, of the largest subunit of RNA polymerase II (RPB1) gene, as well as of concatenated sequences of these two loci (18S-ITS-28S + RPB1) confirmed our hypotheses and proved that both fungi were new species, here described as Dominikia bonfanteae sp. nov. and Glomus atlanticum sp. nov., respectively. In nature, D. bonfanteae was found in a plant community on the shore of Kokotek pond II (Lubliniec in the Silesian Upland, Poland). Glomus atlanticum originated from the Dunes of Aguda Park (Northern Portugal). The phylogenetic position of these new species and their close family relatives within the Glomeraceae were compared and discussed depending on the sequence alignment analysed. This paper for the first time shows the phylogeny of AMF reconstructed from analyses of two concatenated unlinked loci: 18S-ITS-28S plus RPB1. The partial RPB1 gene of D. bonfanteae was amplified using newly designed primers.
Janusz Błaszkowski; Piotr Niezgoda; Szymon Zubek; Edward Meller; Paweł Milczarski; Monika Malicka; Bruno Tomio Goto; Gabriela Woźniak; Helena Moreira; Franco Magurno. Dominikia bonfanteae and Glomus atlanticum, two new species in the Glomeraceae (phylum Glomeromycota) with molecular phylogenies reconstructed from two unlinked loci. Mycological Progress 2021, 20, 131 -148.
AMA StyleJanusz Błaszkowski, Piotr Niezgoda, Szymon Zubek, Edward Meller, Paweł Milczarski, Monika Malicka, Bruno Tomio Goto, Gabriela Woźniak, Helena Moreira, Franco Magurno. Dominikia bonfanteae and Glomus atlanticum, two new species in the Glomeraceae (phylum Glomeromycota) with molecular phylogenies reconstructed from two unlinked loci. Mycological Progress. 2021; 20 (2):131-148.
Chicago/Turabian StyleJanusz Błaszkowski; Piotr Niezgoda; Szymon Zubek; Edward Meller; Paweł Milczarski; Monika Malicka; Bruno Tomio Goto; Gabriela Woźniak; Helena Moreira; Franco Magurno. 2021. "Dominikia bonfanteae and Glomus atlanticum, two new species in the Glomeraceae (phylum Glomeromycota) with molecular phylogenies reconstructed from two unlinked loci." Mycological Progress 20, no. 2: 131-148.
Salt-affected soils are a major problem worldwide for crop production. Bioinocula such as plant growth-promoting bacteria (PGPB) and arbuscular mycorrhizal fungi (AMF) can help plants to thrive in these areas but interactions between them and with soil conditions can modulate the effects on their host. To test potential synergistic effects of bioinoculants with intrinsically different functional relationships with their host in buffering the effect of saline stress, maize plants were grown under increasing soil salinity (0–5 g NaCl kg−-1 soil) and inoculated with two PGPB strains (Pseudomonas reactans EDP28, and Pantoea alli ZS 3-6), one AMF (Rhizoglomus irregulare), and with the combination of both. We then modelled biomass, ion and nutrient content in maize plants in response to increasing salt concentration and microbial inoculant treatments using generalized linear models. The impacts of the different treatments on the rhizosphere bacterial communities were also analyzed. Microbial inoculants tended to mitigate ion imbalances in plants across the gradient of NaCl, promoting maize growth and nutritional status. These effects were mostly prominent in the treatments comprising the dual inoculation (AMF and PGPB), occurring throughout the gradient of salinity in the soil. The composition of bacterial communities of the soil was not affected by microbial treatments and were mainly driven by salt exposure. The tested bioinocula are most efficient for maize growth and health when co-inoculated, increasing the content of K+ accompanied by an effective decrease of Na+ in plant tissues. Moreover, synergistic effects potentially contribute to expanding crop production to otherwise unproductive soils. Results suggest that the combination of AMF and PGPB leads to interactions that may have a potential role in alleviating the stress and improve crop productivity in salt-affected soils.
Helena Moreira; Sofia I.A. Pereira; Alberto López Vega; Paula M.L. Castro; Ana P.G.C. Marques. Synergistic effects of arbuscular mycorrhizal fungi and plant growth-promoting bacteria benefit maize growth under increasing soil salinity. Journal of Environmental Management 2019, 257, 109982 .
AMA StyleHelena Moreira, Sofia I.A. Pereira, Alberto López Vega, Paula M.L. Castro, Ana P.G.C. Marques. Synergistic effects of arbuscular mycorrhizal fungi and plant growth-promoting bacteria benefit maize growth under increasing soil salinity. Journal of Environmental Management. 2019; 257 ():109982.
Chicago/Turabian StyleHelena Moreira; Sofia I.A. Pereira; Alberto López Vega; Paula M.L. Castro; Ana P.G.C. Marques. 2019. "Synergistic effects of arbuscular mycorrhizal fungi and plant growth-promoting bacteria benefit maize growth under increasing soil salinity." Journal of Environmental Management 257, no. : 109982.
The contamination of the soil with heavy metals (e.g. Zn) is a serious and crosscutting issue worldwide. Phytotechnologies can minimize the negative impact of this problem using plants and microorganisms in soil rehabilitation. However, the efficiency of proper plant-microbe combinations is usually assessed using spiked and/or sterilized soils, which do not mimic the conditions in situ, and therefore can lead to outcomes that will not be observed under field situations. This study aimed to quantify the effect of soil origin and sterilization on the performance of the two plant growth promoting rhizobacteria (PGPR), Ralstonia eutropha 1C2 and Chryseobacterium humi ECP37, for promoting the growth and metal accumulation of maize plants. A two-experiment approach was applied: the PGPR were inoculated in maize plants growing in (i) sterilized soils spiked with Zn (0, 100, 500 and 1000 mg Zn kg−1); and in (ii) a field-contaminated soil, under sterilized and non-sterilized conditions (599 mg Zn kg−1). Biomass and Zn accumulation in the root and shoot, and Zn bioavailability in soils were determined. Additionally, lipid peroxidation, activity of antioxidant enzymes catalase (CAT) and superoxide dismutase (SOD) were assessed in the shoots of plants grown in the field-contaminated soil, as well as the composition of the rhizospheric bacterial community. Zn in the soils negatively affected maize growth, and its effect was strongest in the field-contaminated soil. Overall, PGPR attenuated the negative effects of Zn by improving plant growth, although less pronounced in non-sterilized soils. Sterilization significantly reduced soil Zn availability and affected its' accumulation in plant tissues. Bioinoculants performance was also different in sterilized soil, i.e., bacteria had no effect in the accumulation of Zn but tended to increase the biomass of maize plants. Despite the higher Zn accumulation in shoot tissues, lipid peroxidation was lower whereas antioxidant enzymes were enhanced in non-sterilized soils, suggesting that plant antioxidant system functioned properly. PGPR tended to decrease the diversity of the rhizospheric community. This study highlights that while inoculation with PGPR is effective in increasing Zn bioavailability in soil, accumulation in the plant and maize growth in Zn-contaminated soils, the extent of their effect can be different depending on whether the soil is field-contaminated or metal spiked, and on whether is sterilized prior contaminated. Consequently, the effect of bacterial inoculants assessed exclusively in metal spiked soil and/or sterilized soil may be overestimated, and potentially not transferable to field conditions.
Helena Moreira; Sofia I.A. Pereira; Ana P.G.C. Marques; António O.S.S. Rangel; Paula M.L. Castro. Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes. Geoderma 2018, 334, 72 -81.
AMA StyleHelena Moreira, Sofia I.A. Pereira, Ana P.G.C. Marques, António O.S.S. Rangel, Paula M.L. Castro. Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes. Geoderma. 2018; 334 ():72-81.
Chicago/Turabian StyleHelena Moreira; Sofia I.A. Pereira; Ana P.G.C. Marques; António O.S.S. Rangel; Paula M.L. Castro. 2018. "Effects of soil sterilization and metal spiking in plant growth promoting rhizobacteria selection for phytotechnology purposes." Geoderma 334, no. : 72-81.
Soil salinization and fresh water scarcity are amongst the main environmental/agricultural problems, with serious consequences to plant productivity. Amelioration with microorganisms can enhance plant performance under salt conditions. The aim of this work was to evaluate the role of beneficial rhizospheric microorganisms on the growth of sunflower plants irrigated with salinized water with particular attention to nutrient balance and biochemical responses. Sunflower seedlings were inoculated with the arbuscular mycorrhizal fungi Rhizophagus irregularis, the rhizobacteria Chryseobacterium humi ECP37T, or the bacterial endophyte Ochrobacterium haematophilum ZR3-5, and with a mixed inocula of those microorganisms. Plant growth, nutrient accumulation and lipid peroxidation in plant tissues, and the activity of soil enzymes, were evaluated. Irrigating sunflower plants with saline water resulted in decreases in growth and negative effects in salt stress markers, however the application of bioinoculants enhanced biomass production and accumulation of K+, Mg2+, Ca2+, N and P, reduced Na+ levels in tissues and increased plant antioxidative response. This study contributes to devise inoculation strategies for sunflower cultivation in areas prone to salinization.
Sofia I.A. Pereira; Helena Moreira; Konstantinos Argyras; Paula M.L. Castro; Ana P.G.C. Marques. Promotion of sunflower growth under saline water irrigation by the inoculation of beneficial microorganisms. Applied Soil Ecology 2016, 105, 36 -47.
AMA StyleSofia I.A. Pereira, Helena Moreira, Konstantinos Argyras, Paula M.L. Castro, Ana P.G.C. Marques. Promotion of sunflower growth under saline water irrigation by the inoculation of beneficial microorganisms. Applied Soil Ecology. 2016; 105 ():36-47.
Chicago/Turabian StyleSofia I.A. Pereira; Helena Moreira; Konstantinos Argyras; Paula M.L. Castro; Ana P.G.C. Marques. 2016. "Promotion of sunflower growth under saline water irrigation by the inoculation of beneficial microorganisms." Applied Soil Ecology 105, no. : 36-47.
The use of heavy metals (HM) contaminated soils to grow energy crops can diminish the negative impact of HM in the environment improving land restoration. The effect of two PGPR (B1—Chryseobacterium humi ECP37T and B2—Pseudomonas reactans EDP28) and an AMF (F—Rhizophagus irregularis) on growth, Cd and Zn accumulation, and nutritional status of energy maize plants grown in a soil collected from an area adjacent to a Portuguese mine was assessed in a greenhouse experiment. Both bacterial strains, especially when co-inoculated with the AMF, acted as plant growth-promoting inoculants, increasing root and shoot biomass as well as shoot elongation. Cadmium was not detected in the maize tissues and a decrease in Zn accumulation was observed for all microbial treatments in aboveground and belowground tissues—with inoculation of maize with AMF and strain B2 leading to maximum reductions in Zn shoot and root accumulation of up to 48 and 43 %, respectively. Although microbial single inoculation generally did not increase N and P levels in maize plants, co-inoculation of the PGPR and the AMF improved substantially P accumulation in roots. The DGGE analysis of the bacterial rhizosphere community showed that the samples inoculated with the AMF clustered apart of those without the AMF and the Shannon-Wiener Index (H′) increased over the course of the experiment when both inoculants were present. This work shows the benefits of combined inoculation of AMF and PGPR for the growth energy maize in metal contaminated soils and their potential for the application in phytomanagement strategies.
Helena Moreira; Sofia I. A. Pereira; Ana P. G. C. Marques; António O. S. S. Rangel; Paula M. L. Castro. Mine land valorization through energy maize production enhanced by the application of plant growth-promoting rhizobacteria and arbuscular mycorrhizal fungi. Environmental Science and Pollution Research 2015, 23, 6940 -6950.
AMA StyleHelena Moreira, Sofia I. A. Pereira, Ana P. G. C. Marques, António O. S. S. Rangel, Paula M. L. Castro. Mine land valorization through energy maize production enhanced by the application of plant growth-promoting rhizobacteria and arbuscular mycorrhizal fungi. Environmental Science and Pollution Research. 2015; 23 (7):6940-6950.
Chicago/Turabian StyleHelena Moreira; Sofia I. A. Pereira; Ana P. G. C. Marques; António O. S. S. Rangel; Paula M. L. Castro. 2015. "Mine land valorization through energy maize production enhanced by the application of plant growth-promoting rhizobacteria and arbuscular mycorrhizal fungi." Environmental Science and Pollution Research 23, no. 7: 6940-6950.
Zea mays (L.) is a crop widely cultivated throughout the world and can be considered suitable for phytomanagement due to its metal resistance and energetic value. In this study, the effect of two plant growth-promoting rhizobacteria, Ralstonia eutropha and Chryseobacterium humi, on growth and metal uptake of Z. mays plants in soils contaminated with up to 30 mg Cd kg−1 was evaluated. Bacterial inoculation increased plant biomass up to 63 % and led to a decrease of up to 81 % in Cd shoot levels (4–88 mg Cd kg−1) and to an increase of up to 186 % in accumulation in the roots (52–134 mg Cd kg−1). The rhizosphere community structure changed throughout the experiment and varied with different levels of Cd soil contamination, as revealed by molecular biology techniques. Z. mays plants inoculated with either of the tested strains may have potential application in a strategy of soil remediation, in particular short-term phytostabilization, coupled with biomass production for energy purposes.
Helena Moreira; Ana P. G. C. Marques; Albina R. Franco; António O. S. S. Rangel; Paula M. L. Castro. Phytomanagement of Cd-contaminated soils using maize (Zea mays L.) assisted by plant growth-promoting rhizobacteria. Environmental Science and Pollution Research 2014, 21, 9742 -9753.
AMA StyleHelena Moreira, Ana P. G. C. Marques, Albina R. Franco, António O. S. S. Rangel, Paula M. L. Castro. Phytomanagement of Cd-contaminated soils using maize (Zea mays L.) assisted by plant growth-promoting rhizobacteria. Environmental Science and Pollution Research. 2014; 21 (16):9742-9753.
Chicago/Turabian StyleHelena Moreira; Ana P. G. C. Marques; Albina R. Franco; António O. S. S. Rangel; Paula M. L. Castro. 2014. "Phytomanagement of Cd-contaminated soils using maize (Zea mays L.) assisted by plant growth-promoting rhizobacteria." Environmental Science and Pollution Research 21, no. 16: 9742-9753.
Phytoremediation is a promising alternative to conventional soil clean-up methods; however, up to date, there is still not enough information on plant species suitable for application in this field of science. Therefore, plant screening on contaminated sites can lead to the identification of further species of interest. In the present study, pedological and botanical characteristics of an industrialised area known for its metal contamination, in special with Zn—Esteiro de Estarreja, in Portugal—were examined in a 1-year screening. Twenty-seven species were found, with a higher occurrence and variability in the summer/spring season. Zinc levels in the tissues of the collected plant samples ranged from 34 mg kg−1 in shoots to 2,440 mg kg−1 in roots of different species. Species as Verbascum virgatum, Hypochoeris radicata, Phalaris arundinacea, Conyza bilbaoana, Paspalum urvillei and Aster squamatus have shown high Zn shoot accumulation and bioconcentration factors (BCFshoots > 1) and high metal translocation factors (TF > 1). Others, namely Spergularia capillacea, excluded Zn from the shoot tissues and stored the metal at the root zone (BCFroots > 1), behaving as tolerant plants. Plants were also screened for arbuscular mycorrhizal fungi colonisation, and only few species showed mycorrhizal presence, namely C. bilbaoana, Hirschfeldia incana, Epilobium tetragonum, Conyza sumatrensis, Pteridium aquilinum, P. urvillei and A. squamatus. The present work showed important indigenous species that can cope with installed harsh conditions and with potential for utilisation in phytoremediation strategies, either through metal removal to aerial parts or through its immobilisation in the root zone.
Helena Moreira; Ana P. G. C. Marques; António O. S. S. Rangel; Paula M. L. Castro. Heavy Metal Accumulation in Plant Species Indigenous to a Contaminated Portuguese Site: Prospects for Phytoremediation. Water, Air, & Soil Pollution 2011, 221, 377 -389.
AMA StyleHelena Moreira, Ana P. G. C. Marques, António O. S. S. Rangel, Paula M. L. Castro. Heavy Metal Accumulation in Plant Species Indigenous to a Contaminated Portuguese Site: Prospects for Phytoremediation. Water, Air, & Soil Pollution. 2011; 221 (1):377-389.
Chicago/Turabian StyleHelena Moreira; Ana P. G. C. Marques; António O. S. S. Rangel; Paula M. L. Castro. 2011. "Heavy Metal Accumulation in Plant Species Indigenous to a Contaminated Portuguese Site: Prospects for Phytoremediation." Water, Air, & Soil Pollution 221, no. 1: 377-389.
Zea mays, one of the most important cereals worldwide, is a plant not only with food and energy value, but also with phytoremediation potential. The use of plant growth promoting (PGP) rhizobacteria may constitute a biological alternative to increase crop yield and plant resistance to degraded environments. In search for PGP rhizobacteria strains, 6 bacterial isolates were isolated from a metal contaminated site, screened in vitro for their PGP characteristics and their effects on the growth of Z. mays were assessed. Isolates were identified as 3A10T, ECP37T, corresponding to Chryseobacterium palustre and Chryseobacterium humi, and 1ZP4, EC15, EC30 and 1C2, corresponding to strains within the genera Sphingobacterium, Bacillus, Achromobacter, and Ralstonia, respectively. All the bacterial isolates were shown to produce indole acetic acid, hydrogen cyanide and ammonia when tested in vitro for their plant growth promoting abilities, but only isolates 1C2, 1ZP4 and ECP37T have shown siderophore production. Their further application in a greenhouse experiment using Z. mays indicated that plant traits such as root and shoot elongation and biomass production, and nutrient status, namely N and P levels, were influenced by the inoculation, with plants inoculated with 1C2 generally outperforming the other treatments. Two other bacterial isolates, 1ZP4 and ECP37T also led to increased plant growth in the greenhouse. These 3 species, corresponding to strains within the genera Ralstonia (1C2), Sphingobacterium (1ZP4), and to a strain identified as C. humi (ECP37T) can thus be potential agents to increase crop yield in maize plants.
Ana P.G.C. Marques; Carlos Pires; Helena Moreira; António O.S.S. Rangel; Paula M.L. Castro. Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biology and Biochemistry 2010, 42, 1229 -1235.
AMA StyleAna P.G.C. Marques, Carlos Pires, Helena Moreira, António O.S.S. Rangel, Paula M.L. Castro. Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant. Soil Biology and Biochemistry. 2010; 42 (8):1229-1235.
Chicago/Turabian StyleAna P.G.C. Marques; Carlos Pires; Helena Moreira; António O.S.S. Rangel; Paula M.L. Castro. 2010. "Assessment of the plant growth promotion abilities of six bacterial isolates using Zea mays as indicator plant." Soil Biology and Biochemistry 42, no. 8: 1229-1235.
This work investigates the potential of Rubus ulmifolius, indigenous to a metal contaminated site – Esteiro de Estarreja – for phytoremediation purposes. The site has a long history of metal contamination. The accumulation of lead (Pb), arsenic (As) and nickel (Ni) in different sections – roots, stems and leaves – of the plant was assessed and compared to the levels of those metals in the soil and in the available fraction. The distribution of metals throughout the area was quite heterogeneous, presenting levels of As, Pb and Ni of up to 3078, 1400 and 135 mg kg−1, respectively, and the metal content in the sections of R. ulmifolius collected in the banks of the stream varied among sites of collection. Levels of metals were higher in the plant roots: As levels (mg kg−1) ranged from 277 to 1721 in the roots, 30 to 110 in the stems, and 60 to 265 in the leaves; Pb concentrations (mg kg−1) ranged from 248 to 1178 in the roots, 35 to 133 in the stems, and 25 to 149 in the leaves; and Ni (mg kg−1) ranged from 48 to 151 in the roots. Significant correlations were found between the total levels of Pb and As in the soil and the levels in the roots of the plant; further correlations between total and available levels in the soil and metals in other plant tissues were generally found as non-significant. According to the metal accumulation patterns of R. ulmifolius, this species seems to be valuable for application in phytostabilisation strategies.
Ana P.G.C. Marques; Helena Moreira; António O.S.S. Rangel; Paula M.L. Castro. Arsenic, lead and nickel accumulation in Rubus ulmifolius growing in contaminated soil in Portugal. Journal of Hazardous Materials 2009, 165, 174 -179.
AMA StyleAna P.G.C. Marques, Helena Moreira, António O.S.S. Rangel, Paula M.L. Castro. Arsenic, lead and nickel accumulation in Rubus ulmifolius growing in contaminated soil in Portugal. Journal of Hazardous Materials. 2009; 165 (1-3):174-179.
Chicago/Turabian StyleAna P.G.C. Marques; Helena Moreira; António O.S.S. Rangel; Paula M.L. Castro. 2009. "Arsenic, lead and nickel accumulation in Rubus ulmifolius growing in contaminated soil in Portugal." Journal of Hazardous Materials 165, no. 1-3: 174-179.