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The present study aims at developing an efficient bacterial consortium to biodegrade butyric acid, one of the odor-causing compounds that contribute significantly to pit latrine malodors. Six bacterial strains isolated from pit latrine fecal sludge were selected for the study. Nineteen bacterial consortia of different combinations were artificially constructed. The individual bacterial strains and bacterial consortia were compared by culturing in mineral salt medium supplemented with 1000 mg/L butyric acid as a sole carbon and energy source at pH 7, 30 °C, and 110 rpm under aerobic growth conditions. A co-culture of Serratia marcescens and Bacillus cereus was an effective bacterial consortium compared to individual component bacterial strains and other bacterial consortia, in which 1000 mg/L butyric acid was completely degraded within 16 h of incubation. A temperature of 30 °C and pH 7 were found to be optimum for the maximum degradation for both S. marcescens and B. cereus. The inoculation sizes of 2.0 and 2.5 were optimal for the maximum degradation for B. cereus and S. marcescens, respectively. The study provides insights that will be of substantial help in the development of effective biological treatment technologies for pit latrine odor to change the pit latrine user community’s and would be users’ perception of pit latrines.
John Njalam’Mano; Evans Chirwa; Refilwe Seabi. In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge. Sustainability 2020, 12, 5156 .
AMA StyleJohn Njalam’Mano, Evans Chirwa, Refilwe Seabi. In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge. Sustainability. 2020; 12 (12):5156.
Chicago/Turabian StyleJohn Njalam’Mano; Evans Chirwa; Refilwe Seabi. 2020. "In Vitro Study of Butyric Acid Deodorization Potential by Indigenously Constructed Bacterial Consortia and Pure Cultures from Pit Latrine Fecal Sludge." Sustainability 12, no. 12: 5156.
Butyric acid is one of the volatile organic compounds that significantly contribute to malodour emission from pit latrines. The purpose of this work is to isolate and identify bacterial strains that have the capability to degrade butyric acid, determine their butyric acid degradation efficiencies and estimate their growth pattern parameters of microbiological relevance. Pure cultures of bacterial strains capable of degrading butyric acid were isolated from pit latrine faecal sludge using an enrichment technique and were identified based on 16S rRNA analysis. The bacterial strains were cultured in mineral salt medium (MSM) supplemented with 1000 mg L−1 butyric acid, as a sole carbon and energy source, at 30 ± 1 °C, pH 7 and 110 rpm under aerobic growth conditions. The modified Gompertz model was used to estimate growth pattern parameters of microbiological relevance. Bacterial strains were phylogenetically identified as Alcaligenes sp. strain SY1, Achromobacter animicus, Pseudomonas aeruginosa, Serratia marcescens, Achromobacter xylosoxidans, Bacillus cereus, Lysinibacillus fusiformis, Bacillus methylotrophicus and Bacillus subtilis. The bacterial strains in pure cultures degraded butyric acid of 1000 mg L−1 within 20–24 h. The growth kinetics of the bacterial strains in pure culture utilising butyric acid were well described by the modified Gompertz model. This work highlights the potential for use of these bacterial strains in microbial degradation of butyric acid for deodorisation of pit latrine faecal sludge. This work also contributes significantly to our understanding of bioremediation of faecal sludge odours and informs the development of appropriate odour control technologies that may improve odour emissions from pit latrines.
John Bright J. Njalam’Mano; Evans Martin N. Chirwa. Indigenous butyric acid-degrading bacteria as surrogate pit latrine odour control: isolation, biodegradability performance and growth kinetics. Annals of Microbiology 2018, 69, 107 -122.
AMA StyleJohn Bright J. Njalam’Mano, Evans Martin N. Chirwa. Indigenous butyric acid-degrading bacteria as surrogate pit latrine odour control: isolation, biodegradability performance and growth kinetics. Annals of Microbiology. 2018; 69 (2):107-122.
Chicago/Turabian StyleJohn Bright J. Njalam’Mano; Evans Martin N. Chirwa. 2018. "Indigenous butyric acid-degrading bacteria as surrogate pit latrine odour control: isolation, biodegradability performance and growth kinetics." Annals of Microbiology 69, no. 2: 107-122.