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Nesterenkonia sp. PF2B19, a psychrophile was isolated from 44,800-year-old permafrost soil. This is the first report on comparative genomics of Nesterenkonia sp. isolated from Arctic. Genome of PF2B19 exhibited the presence of a vast array of genetic determinants involved in cold adaptation i.e., response to cold-associated general, osmotic, and oxidative stress. These genomic attributes proved to be valuable in unraveling the adaptive tactics employed by PF2B19 for survival in the cold permafrost soils of the Arctic. Genomic analysis of PF2B19 has given some valuable insight into the biotechnological potential of this strain, particularly as a source of cold-active enzymes, as a bioremediating agent and as plant growth-promoting bacteria.
Purnima Singh; Neelam Kapse; Vasudevan Gowdaman; Masaharu Tsuji; Shiv Singh; Prashant Dhakephalkar. Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential. Sustainability 2021, 13, 4590 .
AMA StylePurnima Singh, Neelam Kapse, Vasudevan Gowdaman, Masaharu Tsuji, Shiv Singh, Prashant Dhakephalkar. Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential. Sustainability. 2021; 13 (8):4590.
Chicago/Turabian StylePurnima Singh; Neelam Kapse; Vasudevan Gowdaman; Masaharu Tsuji; Shiv Singh; Prashant Dhakephalkar. 2021. "Comparative Genomic Analysis of Arctic Permafrost Bacterium Nesterenkonia sp. PF2B19 to Gain Insights into Its Cold Adaptation Tactic and Diverse Biotechnological Potential." Sustainability 13, no. 8: 4590.
To understand the microbial composition and diversity patterns, cryoconite granules were collected from two geographical areas, i.e., Nepali Himalaya and Greenland, Arctic. 16S rRNA, ITS and the D1/D2 domain sequencing techniques were used for characterization of microbial communities of the four glaciers. The total 13 species of bacteria such as Bacillus aryabhattai, Bacillus simplex, Brevundimonas vesicularis, Cryobacterium luteum, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Glaciihabitans tibetensis, Leifsonia kafniensis, Paracoccus limosus, Polaromonas glacialis, Sporosarcina globispora, Staphylococcus saprophyticus, Variovorax ginsengisoli, and 4 species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Dothideomycetes sp., Helotiales sp. were recorded from Nepali Himalaya. Among these, 12 species of bacteria and 4 species of fungi are new contributions to Himalaya. In contrast to this, six species of bacteria such as Bacillus cereus, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Enhydrobacter aerosaccus, Glaciihabitans tibetensis, Subtercola frigoramans, and nine species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Naganishia vaughanmartiniae, Piskurozyma fildesensis, Rhodotorula svalbardensis, Alatospora acuminata, Articulospora sp., Phialophora sp., Thelebolus microspores, and Dothideomycetes sp.), were recorded from Qaanaaq, Isunnguata Sermia and Thule glaciers, Greenland. Among these, five species of bacteria and seven species of fungi are new contributions to Greenland cryoconite. Microbial analyses indicate that the Nepali Himalayan cryoconite colonize higher numbers of microbial species compared to the Greenland cryoconite.
Purnima Singh; Masaharu Tsuji; Shiv Mohan Singh; Nozomu Takeuchi. Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic. Sustainability 2020, 12, 6477 .
AMA StylePurnima Singh, Masaharu Tsuji, Shiv Mohan Singh, Nozomu Takeuchi. Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic. Sustainability. 2020; 12 (16):6477.
Chicago/Turabian StylePurnima Singh; Masaharu Tsuji; Shiv Mohan Singh; Nozomu Takeuchi. 2020. "Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic." Sustainability 12, no. 16: 6477.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Site U1521. Volume 353: Indian Monsoon Rainfall 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Site U1521. Volume 353: Indian Monsoon Rainfall. 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Site U1521." Volume 353: Indian Monsoon Rainfall , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Expedition 374 summary. Hikurangi Subduction Margin Coring, Logging, and Observatories 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Expedition 374 summary. Hikurangi Subduction Margin Coring, Logging, and Observatories. 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Expedition 374 summary." Hikurangi Subduction Margin Coring, Logging, and Observatories , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Site U1525. Volume 353: Indian Monsoon Rainfall 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Site U1525. Volume 353: Indian Monsoon Rainfall. 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Site U1525." Volume 353: Indian Monsoon Rainfall , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Site U1523. Volume 353: Indian Monsoon Rainfall 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Site U1523. Volume 353: Indian Monsoon Rainfall. 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Site U1523." Volume 353: Indian Monsoon Rainfall , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Expedition 374 methods. Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC) 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Expedition 374 methods. Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC). 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Expedition 374 methods." Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC) , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Site U1524. Volume 353: Indian Monsoon Rainfall 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Site U1524. Volume 353: Indian Monsoon Rainfall. 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Site U1524." Volume 353: Indian Monsoon Rainfall , no. : 1.
R.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. Site U1522. Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC) 2019, 1 .
AMA StyleR.M. McKay, L. De Santis, D.K. Kulhanek, J.L. Ash, F. Beny, I.M. Browne, G. Cortese, I.M. Cordeiro De Sousa, J.P. Dodd, O.M. Esper, J.A. Gales, D.M. Harwood, S. Ishino, B.A. Keisling, S. Kim, J.S. Laberg, R.M. Leckie, J. Müller, M.O. Patterson, B.W. Romans, O.E. Romero, F. Sangiorgi, O. Seki, A.E. Shevenell, S.M. Singh, S.T. Sugisaki, T. Van De Flierdt, T.E. Van Peer, W. Xiao, Z. Xiong. Site U1522. Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC). 2019; ():1.
Chicago/Turabian StyleR.M. McKay; L. De Santis; D.K. Kulhanek; J.L. Ash; F. Beny; I.M. Browne; G. Cortese; I.M. Cordeiro De Sousa; J.P. Dodd; O.M. Esper; J.A. Gales; D.M. Harwood; S. Ishino; B.A. Keisling; S. Kim; J.S. Laberg; R.M. Leckie; J. Müller; M.O. Patterson; B.W. Romans; O.E. Romero; F. Sangiorgi; O. Seki; A.E. Shevenell; S.M. Singh; S.T. Sugisaki; T. Van De Flierdt; T.E. Van Peer; W. Xiao; Z. Xiong. 2019. "Site U1522." Volume 383: Dynamics of the Pacific Antarctic Circumpolar Current (DYNAPACC) , no. : 1.
A psychrotolerant yeast strain Mrakia robertii A2‐3 isolated from cryoconites of Hamtah glacier, Himalaya, India was investigated for the production of cold tolerant endoglucanase. Optimum endoglucanase production was found at 15 °C with an initial pH of 5.5, and potent inducers were 1% w/v of xylose and KNO3 and 0.1% w/v of NaCl. Under the optimum conditions, enzyme production was 1.81 fold higher than the un‐optimized conditions. Crude enzyme was partially purified by ammonium sulphate precipitation followed by dialysis. The enzyme was purified to 2.53 fold and yield was 6.03% with specific activity of 17.38 U/mg and molecular weight ~57 kDa. The Km and Vmax values of the partially purified enzyme were found to be 1.57 mg/ml and 142.85 U/mg, respectively. The characterization study revealed that the best temperature was 15°C for activity and stability. Furthermore enzyme showed the highest activity at pH 11.0 and was stable at pH6.0. Fe2+, Mn2+, Na2+, Cu2+, Co2+, Ca2+ proved to be activators of endoglucanase. EDTA showed very low effect on the enzyme activity whereas it was active with Tween‐80 and sodium deoxycholate. The present study successfully produced a cold‐active endoglucanase with novel properties making it promising as a biocatalyst for industrial processes. This article is protected by copyright. All rights reserved.
Gandhali M. Dhume; Abhas K. Maharana; Masaharu Tsuji; Alok K. Srivastava; Shiv Mohan Singh. Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya. Journal of Basic Microbiology 2019, 59, 667 -679.
AMA StyleGandhali M. Dhume, Abhas K. Maharana, Masaharu Tsuji, Alok K. Srivastava, Shiv Mohan Singh. Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya. Journal of Basic Microbiology. 2019; 59 (7):667-679.
Chicago/Turabian StyleGandhali M. Dhume; Abhas K. Maharana; Masaharu Tsuji; Alok K. Srivastava; Shiv Mohan Singh. 2019. "Cold-tolerant endoglucanase producing ability of Mrakia robertii A2-3 isolated from cryoconites, Hamtha glacier, Himalaya." Journal of Basic Microbiology 59, no. 7: 667-679.
Cold active amylase was investigated by bacteria and yeast isolates from the sediment core samples of Nella Lake,Larsemann Hills region, East Antarctica. Between potential yeast and bacteria isolates screened for amylases, best isolates were identified asRhodotorula sp. Y-37 and ArthrobacteralpinusN16 by molecular technique.Amylase production capabilities of both the isolate subjected for optimization processes by using submerged fermentation technique with soluble starch as substrate.The results indicate that a supplement of 1% w/v glucose, 1% w/v yeast extract and 0.1% w/v KCl at pH 7.0with 5% v/v inoculum enhances the amylase production by 5.72-fold using Rhodotorula sp. Y-37. In other hands, the activators are 1% w/v of galactose and peptone, 0.1% w/v KCl and 2.5% v/v inoculum at pH 7.0 enhances the amylase production by 3.74-fold using ArthrobacteralpinusN16. Cold-active amylasecan be used in detergent, textile, food and beverage industries. Bio-degradation of starchy materials by cold active amylases can contribute in cleaning of environment at cold regions without harming the climate.
Abhas Kumar Maharana; Shiv Mohan Singh. Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica. Biosciences Biotechnology Research Asia 2018, 15, 05 -16.
AMA StyleAbhas Kumar Maharana, Shiv Mohan Singh. Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica. Biosciences Biotechnology Research Asia. 2018; 15 (1):05-16.
Chicago/Turabian StyleAbhas Kumar Maharana; Shiv Mohan Singh. 2018. "Cold Active Amylases Producing Psychrotolerants Isolated from Nella Lake, Antarctica." Biosciences Biotechnology Research Asia 15, no. 1: 05-16.
Psychrotolerant yeast Rhodotorula sp. Y-23 was isolated from the sediment core sub-samples of Nella Lake, East Antarctica. Isolate was screened for lipase production using plate assay method followed by submerged fermentation. Production optimization revealed the maximum lipase production by using palmolein oil (5% v/v), pH 8.0 and inoculum size of 2.5% v/v at 15 °C. The potential inducers for lipase were 1% w/v of galactose and KNO3, and MnCl2 (0.1% w/v). Final productions with optimized conditions gave 5.47-fold increase in lipase production. Dialyzed product gave a purification fold of 5.63 with specific activity of 26.83 U mg−1 and 15.67% yields. This lipase was more stable at pH 5.0 and −20 °C whereas more activity was found at pH 8.0 and 35 °C. Stability was more in 50 mM Fe3+, EDTA-Na (20 mM), sodium deoxycholate (20 mM), H2O2 (1% v/v), and almost all organic solvents (50% v/v). Tolerance capacity at wider range of pH and temperature with having lower Km value i.e., 0.08 mg ml−1 and higher Vmax 385.68 U mg−1 at 15 °C make the studied lipase useful for industrial applications. Besides this, the lipase was compatible with commercially available detergents, and its addition to them increases lipid degradation performances making it a potential candidate in detergent formulation.
Abhas K. Maharana; Shiv M. Singh. A cold and organic solvent tolerant lipase produced by Antarctic strainRhodotorulasp. Y-23. Zeitschrift für allgemeine Mikrobiologie 2018, 58, 331 -342.
AMA StyleAbhas K. Maharana, Shiv M. Singh. A cold and organic solvent tolerant lipase produced by Antarctic strainRhodotorulasp. Y-23. Zeitschrift für allgemeine Mikrobiologie. 2018; 58 (4):331-342.
Chicago/Turabian StyleAbhas K. Maharana; Shiv M. Singh. 2018. "A cold and organic solvent tolerant lipase produced by Antarctic strainRhodotorulasp. Y-23." Zeitschrift für allgemeine Mikrobiologie 58, no. 4: 331-342.
Permafrost soils are unique habitats in polar environment and are of great ecological relevance. The present study focuses on the characterization of bacterial communities from permafrost profiles of Svalbard, Arctic. Counts of culturable bacteria range from 1.50 × 103 to 2.22 × 105 CFU g−1, total bacterial numbers range from 1.14 × 105 to 5.52 × 105 cells g−1 soil. Bacterial isolates are identified through 16S rRNA gene sequencing. Arthrobacter and Pseudomonas are the most dominant genera, and A. sulfonivorans, A. bergeri, P. mandelii, and P. jessenii as the dominant species. Other species belong to genera Acinetobacter, Bacillus, Enterobacter, Nesterenkonia, Psychrobacter, Rhizobium, Rhodococcus, Sphingobacterium, Sphingopyxis, Stenotrophomonas, and Virgibacillus. To the best of our knowledge, genera Acinetobacter, Enterobacter, Nesterenkonia, Psychrobacter, Rhizobium, Sphingobacterium, Sphingopyxis, Stenotrophomonas, and Virgibacillus are the first northernmost records from Arctic permafrost. The present study fills the knowledge gap of culturable bacterial communities and their chronological characterization from permafrost soils of Ny-Ålesund (79°N), Arctic.
Purnima Singh; Shiv M. Singh; Ram N. Singh; Simantini Naik; Utpal Roy; Alok Srivastava; Manfred Bölter. Bacterial communities in ancient permafrost profiles of Svalbard, Arctic. Journal of Basic Microbiology 2017, 57, 1018 -1036.
AMA StylePurnima Singh, Shiv M. Singh, Ram N. Singh, Simantini Naik, Utpal Roy, Alok Srivastava, Manfred Bölter. Bacterial communities in ancient permafrost profiles of Svalbard, Arctic. Journal of Basic Microbiology. 2017; 57 (12):1018-1036.
Chicago/Turabian StylePurnima Singh; Shiv M. Singh; Ram N. Singh; Simantini Naik; Utpal Roy; Alok Srivastava; Manfred Bölter. 2017. "Bacterial communities in ancient permafrost profiles of Svalbard, Arctic." Journal of Basic Microbiology 57, no. 12: 1018-1036.
Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old permafrost. The draft genome sequence of this strain revealed the presence of genes involved in the production of cold active enzymes, carotenoid biosynthesis, fatty acid biosynthesis, and resistance to heavy metals. These results show the immense potential of the strain.
Purnima Singh; Neelam Kapse; Utpal Roy; Shiv Mohan Singh; P. K. Dhakephalkar. Draft Genome Sequence of Permafrost Bacterium Nesterenkonia sp. Strain PF2B19, Revealing a Cold Adaptation Strategy and Diverse Biotechnological Potential. Genome Announcements 2017, 5, e00133-17 .
AMA StylePurnima Singh, Neelam Kapse, Utpal Roy, Shiv Mohan Singh, P. K. Dhakephalkar. Draft Genome Sequence of Permafrost Bacterium Nesterenkonia sp. Strain PF2B19, Revealing a Cold Adaptation Strategy and Diverse Biotechnological Potential. Genome Announcements. 2017; 5 (15):e00133-17.
Chicago/Turabian StylePurnima Singh; Neelam Kapse; Utpal Roy; Shiv Mohan Singh; P. K. Dhakephalkar. 2017. "Draft Genome Sequence of Permafrost Bacterium Nesterenkonia sp. Strain PF2B19, Revealing a Cold Adaptation Strategy and Diverse Biotechnological Potential." Genome Announcements 5, no. 15: e00133-17.
Despite feather fungi being an important component of the Arctic fungal flora, their ecological role and diversity are not fully known. In the current study, fungal cultures were isolated from feathers (barnacle goose, common eider, and glaucous gull) collected in the Ny-Ålesund region, Svalbard. Isolates were identified by ITS region sequences, which include the ITS1, ITS2, and 5.8S rRNA. The result showed culturable yeast and filamentous fungi belonging to three classes: Ascomycota (Pyrenochaetopsis pratorum, Cladosporium herbarum, Thelebolus microsporus, Aspergillus versicolor, Penicillium commune, and Venturia sp.), Basidiomycota (Mrakia blollopis and Rhodotorula mucilaginosa), and Zygomycota (Mucor flavus). Most of the fungal isolates appeared to be cold-tolerant, and about 60 % of the isolates showed keratinase activity. The reasonably low fungal diversity colonizing feathers indicates that the birds of Svalbard are casual carriers of fungi which may result in a negligible impact on their health. To the best of our knowledge, this is the first record of fungal communities present on the feathers of birds in the high Arctic.
Shiv M. Singh; Masaharu Tsuji; Puja Gawas-Sakhalker; Maarten J.J.E. Loonen; Tamotsu Hoshino. Bird feather fungi from Svalbard Arctic. Polar Biology 2015, 39, 523 -532.
AMA StyleShiv M. Singh, Masaharu Tsuji, Puja Gawas-Sakhalker, Maarten J.J.E. Loonen, Tamotsu Hoshino. Bird feather fungi from Svalbard Arctic. Polar Biology. 2015; 39 (3):523-532.
Chicago/Turabian StyleShiv M. Singh; Masaharu Tsuji; Puja Gawas-Sakhalker; Maarten J.J.E. Loonen; Tamotsu Hoshino. 2015. "Bird feather fungi from Svalbard Arctic." Polar Biology 39, no. 3: 523-532.
Lichens and cryoconite (rounded or granular, brownish-black debris occurring in holes on the glacier surface) from Ny-Ålesund were used for understanding the elemental deposition pattern in the area. Lichen samples collected from low-lying coastal region and cryoconite samples from high altitudinal glacier area were processed and analysed for elements such as aluminium (Al), arsenic (As), cadmium (Cd), cobalt (Co), chromium (Cr), cesium (Cs), copper (Cu), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb), vanadium (V) and zinc (Zn) through inductively coupled plasma mass spectrometry. Results showed that heavy metals, Al and Fe, are present in high concentration in the cryoconite samples. Al was also present in high amounts in seven of the eight lichen samples studied. The general scheme of elements in the decreasing order of their concentrations for most of the cryoconite samples was Al > Fe > Mn > Zn > V > Pb > Cr > Ni > Cu > Co > As > Cs > Cd while that for the lichen samples was Al > Fe > Zn > Mn > Pb > Cu > Cs > Cr > Ni > V > Co > As > Cd. Similarity in trends in the two sample types confirms that the environment indeed contains these elements in that order of concentration which overtime got accumulated in the samples. Overall comparison showed most elements to be present in high concentrations in the cryoconite samples as compared to the lichen samples. Within the lichens, elemental accumulation data suggests that the low-lying site (L-2) from where Cladonia mediterranea sample was collected was the most polluted accumulating a number of elements at high concentrations. The probable reasons for such deposition patterns in the region could be natural (crustal contribution and sea salt spray) and anthropogenic (local and long-distance transmission of dust particles). In the future, this data can form a baseline for monitoring quantum of atmospheric heavy metal deposition in lichens and cryoconite of Svalbard, Arctic.
Shiv Mohan Singh; Jagdev Sharma; Puja Gawas-Sakhalkar; Ajay K. Upadhyay; Simantini Naik; Shailesh M. Pedneker; Rasik Ravindra. Atmospheric deposition studies of heavy metals in Arctic by comparative analysis of lichens and cryoconite. Environmental Monitoring and Assessment 2012, 185, 1367 -1376.
AMA StyleShiv Mohan Singh, Jagdev Sharma, Puja Gawas-Sakhalkar, Ajay K. Upadhyay, Simantini Naik, Shailesh M. Pedneker, Rasik Ravindra. Atmospheric deposition studies of heavy metals in Arctic by comparative analysis of lichens and cryoconite. Environmental Monitoring and Assessment. 2012; 185 (2):1367-1376.
Chicago/Turabian StyleShiv Mohan Singh; Jagdev Sharma; Puja Gawas-Sakhalkar; Ajay K. Upadhyay; Simantini Naik; Shailesh M. Pedneker; Rasik Ravindra. 2012. "Atmospheric deposition studies of heavy metals in Arctic by comparative analysis of lichens and cryoconite." Environmental Monitoring and Assessment 185, no. 2: 1367-1376.
Culturable bacterial diversity of seven marine sediment samples of Kongsfjorden and a sediment and a soil sample from Ny-Ålesund, Svalbard, Arctic was studied. The bacterial abundance in the marine sediments of Kongsfjorden varied marginally (0.5 × 103–1.3 × 104 cfu/g sediment) and the bacterial number in the two samples collected from the shore of Ny-Ålesund also was very similar (0.6 × 104 and 3.4 × 104, respectively). From the nine samples a total of 103 bacterial isolates were obtained and these isolates could be grouped in to 47 phylotypes based on the 16S rRNA gene sequence belonging to 4 phyla namely Actinobacteria, Bacilli, Bacteroidetes and Proteobacteria. Representatives of the 47 phylotypes varied in their growth temperature range (4–37°C), in their tolerance to NaCl (0.3–2 M NaCl) and growth pH range (2–11). Representatives of 26 phylotypes exhibited amylase and lipase activity either at 5 or 20°C or at both the temperatures. A few of the representatives exhibited amylase and/or lipase activity only at 5°C. None of the phylotypes exhibited protease activity. Most of the phylotypes (38) were pigmented. Fatty acid profile studies indicated that short chain fatty acids, unsaturated fatty acids, branched fatty acids, the cyclic and the cis fatty acids are predominant in the psychrophilic bacteria.
T. N. R. Srinivas; S. S. S. Nageswara Rao; P. Vishnu Vardhan Reddy; M. S. Pratibha; B. Sailaja; B. Kavya; K. Hara Kishore; Z. Begum; S. M. Singh; S. Shivaji. Bacterial Diversity and Bioprospecting for Cold-Active Lipases, Amylases and Proteases, from Culturable Bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Current Microbiology 2009, 59, 537 -547.
AMA StyleT. N. R. Srinivas, S. S. S. Nageswara Rao, P. Vishnu Vardhan Reddy, M. S. Pratibha, B. Sailaja, B. Kavya, K. Hara Kishore, Z. Begum, S. M. Singh, S. Shivaji. Bacterial Diversity and Bioprospecting for Cold-Active Lipases, Amylases and Proteases, from Culturable Bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic. Current Microbiology. 2009; 59 (5):537-547.
Chicago/Turabian StyleT. N. R. Srinivas; S. S. S. Nageswara Rao; P. Vishnu Vardhan Reddy; M. S. Pratibha; B. Sailaja; B. Kavya; K. Hara Kishore; Z. Begum; S. M. Singh; S. Shivaji. 2009. "Bacterial Diversity and Bioprospecting for Cold-Active Lipases, Amylases and Proteases, from Culturable Bacteria of Kongsfjorden and Ny-Ålesund, Svalbard, Arctic." Current Microbiology 59, no. 5: 537-547.
The Antarctic habitats are some of the driest and coldest ecosystems on the Earth. Earlier there was a general acceptance that polar deserts harbored little life (Priscu, 1999). But, recent studies have revealed the existence of microbes in: the snow near the South Pole (Carpenter et al., 2000), the 3.5 km deep in Vostok ice (Karl et al., 1999; Priscu et al., 1999a), exposed soils (Wall and Virginia, 1998), sandstones (Friedmann et al., 1993), meltwater ponds (Vincent, 1988), liquid water column of permanently ice-covered lakes (Priscu et al., 1999b), and the ice covers of permanent lake ice (Priscu et al., 1998; Psenner et al., 1999). Most of the microbes found in these habitats are prokaryotic (Vincent, 1988; Gordon et al., 2000; Brambilla et al., 2001). Among these microbes, one of the most important components is the photosynthetically active cyanobacteria. They provide for an adequate quantity of fixed carbon via photosynthesis to drive a well-developed ecosystem (Vincent, 1988). On the contrary, in those habitats where there is a lack of cyanobacteria, biomass production by the addition of new carbon and nitrogen is slowed. Thus, such habitats are poor in biodiversity and also poor in trophic levels. In Antarctic habitats the cyanobacteria are adapted and acclimated to their environment in terms of temperature, freeze/thaw survival photoprotection, as well as light acquisition for photosynthesis (Vincent et al., 1993a, b, c; Tang et al., 1997; Nadeau et al., 1999; Tang and Vincent, 1999; Nadeau and Castenholz, 2000). Though cyanobacteria play a significant role in ecosystem dynamics, only a few of them have been considered true psychrophiles (Tang et al., 1997; Fritsen and Priscu, 1998). They are classified as psychrotolerant or psychrotrophic due to their ability to metabolize near 0ºC and also because their temperature optima for growth are typically above 15ºC. Some of the cyanobacterial groups, for example, Leptolyngbya, Phormidium, Oscillatoria, and Nostoc are cosmopolitan and occur in highly divergent environmental extremes.
S. M. Singh; J. Elster; Joseph Seckbach. Cyanobacteria in Antarctic Lake Environments. Cellular Origin, Life in Extreme Habitats and Astrobiology 2007, 11, 303 -320.
AMA StyleS. M. Singh, J. Elster, Joseph Seckbach. Cyanobacteria in Antarctic Lake Environments. Cellular Origin, Life in Extreme Habitats and Astrobiology. 2007; 11 ():303-320.
Chicago/Turabian StyleS. M. Singh; J. Elster; Joseph Seckbach. 2007. "Cyanobacteria in Antarctic Lake Environments." Cellular Origin, Life in Extreme Habitats and Astrobiology 11, no. : 303-320.