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Hymenoptera venom allergy significantly affects the quality of life. Due to the divergences in the results of the available test and clinical symptoms of patients, the current widely applied diagnostic methods are often insufficient to classify patients for venom immunotherapy (VIT). Therefore it is still needed to search for new, more precise, and accurate diagnostic methods. Hence, this research aimed to discover new biomarkers of Hymenoptera venom allergy in a group of inflammation factors using set of multi-marker Bioplex panel. The adoption of a novel methodology based on Luminex/xMAP enabled simultaneous determination of serum levels of 37 different inflammatory proteins in one experiment. The study involved 21 patients allergic to wasp and/or honey bee venom and 42 healthy participants. According to univariate and multivariate statistics, soluble CD30/tumor necrosis factor receptor superfamily, member 8 (sCD30/TNFRSF8), and the soluble tumor necrosis factor receptor 1 (sTNF-R1) may be considered as effective prognostic factors, their circulating levels were significantly decreased in the allergy group (p-value < 0.05; the Area Under the Curve (AUC) ~0.7; Variable Importance in Projection (VIP) scores >1.2). The obtained results shed new light on the allergic inflammatory response and may contribute to modification and improvement of the diagnostic and monitoring methods. Further, large-scale studies are still needed to explain mechanisms of action of studied compounds and to definitively prove their usefulness in clinical practice.
Kacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health 2021, 18, 4011 .
AMA StyleKacper Packi, Joanna Matysiak, Eliza Matuszewska, Anna Bręborowicz, Zdzisława Kycler, Jan Matysiak. New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors. International Journal of Environmental Research and Public Health. 2021; 18 (8):4011.
Chicago/Turabian StyleKacper Packi; Joanna Matysiak; Eliza Matuszewska; Anna Bręborowicz; Zdzisława Kycler; Jan Matysiak. 2021. "New Biomarkers of Hymenoptera Venom Allergy in a Group of Inflammation Factors." International Journal of Environmental Research and Public Health 18, no. 8: 4011.
Asthma often begins in childhood, although making an early diagnosis is difficult. Clinical manifestations, the exclusion of other causes of bronchial obstruction, and responsiveness to anti-inflammatory therapy are the main tool of diagnosis. However, novel, precise, and functional biochemical markers are needed in the differentiation of asthma phenotypes, endotypes, and creating personalized therapy. The aim of the study was to search for metabolomic-based asthma biomarkers among free amino acids (AAs). A wide panel of serum-free AAs in asthmatic children, covering both proteinogenic and non-proteinogenic AAs, were analyzed. The examination included two groups of individuals between 3 and 18 years old: asthmatic children and the control group consisted of children with neither asthma nor allergies. High-performance liquid chromatography combined with tandem mass spectrometry (LC-MS/MS technique) was used for AA measurements. The data were analyzed by applying uni- and multivariate statistical tests. The obtained results indicate the decreased serum concentration of taurine, L-valine, DL-β-aminoisobutyric acid, and increased levels of ƴ-amino-n-butyric acid and L-arginine in asthmatic children when compared to controls. The altered concentration of these AAs can testify to their role in the pathogenesis of childhood asthma. The authors’ results should contribute to the future introduction of new diagnostic markers into clinical practice.
Joanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health 2020, 17, 4758 .
AMA StyleJoanna Matysiak, Agnieszka Klupczynska, Kacper Packi, Anna Mackowiak-Jakubowska, Anna Bręborowicz, Olga Pawlicka, Katarzyna Olejniczak, Zenon J. Kokot, Jan Matysiak. Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma. International Journal of Environmental Research and Public Health. 2020; 17 (13):4758.
Chicago/Turabian StyleJoanna Matysiak; Agnieszka Klupczynska; Kacper Packi; Anna Mackowiak-Jakubowska; Anna Bręborowicz; Olga Pawlicka; Katarzyna Olejniczak; Zenon J. Kokot; Jan Matysiak. 2020. "Alterations in Serum-Free Amino Acid Profiles in Childhood Asthma." International Journal of Environmental Research and Public Health 17, no. 13: 4758.