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Prof. Dr. Ahmad Hama Rashid
University of Sulaimani

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0 Agriculture
0 Biotechnology
0 Plant Tissue Culture
0 callus
0 Rice crop

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Journal article
Published: 28 June 2020 in Applied Sciences
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Rice (Oryza sativa L.) is productively affected by different environmental factors, including biotic and abiotic stress. The objectives of this research were to evaluate the genetic distinction among Kurdish rice genotypes using the simple sequence repeats (SSRs) molecular markers and to perform in vitro tests to characterize the drought tolerance of six local rice genotypes. The polymorphic information content (PIC) varied from 0.38 to 0.84 with an average of 0.56. The genetic distance ranged from 0.33 to 0.88. Drought stress had a significant impact (p ≤ 0.05) on callus growth parameters. Enzymatic antioxidant systems were predicted and exhibited a significant variation. The findings revealed that proline levels increase in proportion to polyethylene glycol (PEG) concentrations. Kalar and Gwll Swr genotypes showed the worst performances in phenotypic and biochemical traits, while Choman and Shawre exhibited the best phenotypic and biochemical performances. A positive and substantial relationship between callus fresh weight (CFW) and callus dry weight (CDW) was found under stressful and optimized conditions. Callus induction (CI) was positively and significantly associated with the catalase activity (CAT) in all stressed treatments. Based on the results for callus growth and the biochemical parameters under stress conditions, a remarkable genotype distinction, based on the tolerance reaction, was noted as follows: PEG resistant > susceptible, Choman > Shawre > White Bazyan > Red Bazyan > Gwll Swr > Kalar. The CI and CAT characteristics were considered as reliable predictors of drought tolerance in rice genotypes.

ACS Style

Didar Rahim; Petr Kalousek; Nawroz Tahir; Tomáš Vyhnánek; Petr Tarkowski; Vaclav Trojan; Dana Abdulkhaleq; Ahmad Hama Ameen; Ladislav Havel. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Applied Sciences 2020, 10, 4471 .

AMA Style

Didar Rahim, Petr Kalousek, Nawroz Tahir, Tomáš Vyhnánek, Petr Tarkowski, Vaclav Trojan, Dana Abdulkhaleq, Ahmad Hama Ameen, Ladislav Havel. In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress. Applied Sciences. 2020; 10 (13):4471.

Chicago/Turabian Style

Didar Rahim; Petr Kalousek; Nawroz Tahir; Tomáš Vyhnánek; Petr Tarkowski; Vaclav Trojan; Dana Abdulkhaleq; Ahmad Hama Ameen; Ladislav Havel. 2020. "In Vitro Assessment of Kurdish Rice Genotypes in Response to PEG-Induced Drought Stress." Applied Sciences 10, no. 13: 4471.

Journal article
Published: 28 July 2019 in Applied Sciences
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The volume and complexity of commercial bioreactors for sterile hydroponics and hairy roots are too large for comparative analysis of many cultures. Here a small-scale bioreactor fabricated from standard glass materials and suitable for both airlift and bubble aeration mode is described. The performance of the bioreactor was tested by growing oilseed rape (Brassica napus L.) and rose plants (Rosa canina L.) in sterile hydroponics and by cultivating hairy roots of henbane (Hyoscyamus niger L.) and sesame (Hyoscyamus niger L.). Plants grown in hydroponics for up to six weeks did not show chloroses or necroses. Hairy roots grew faster or comparably fast in bioreactors as compared to shaking flasks. Root exudates of roses and exudates of hairy roots of henbane were subjected to targeted and nontargeted analysis by HPLC coupled with optical and mass spectrometric detectors. The diversity and concentration of hairy root exudates were higher in bioreactors than in shaking flasks. The composition of hairy root exudates of three accessions of H. niger did not match the genetic relatedness among the accessions. Hairy roots of Hyoscyamus niger exuded salicylic acid in amounts varying among plant accessions and between bioreactors and shaking flask cultures.

ACS Style

Zana Jamal Kareem; Ling Su; Anna Rathgeb; Anke Sirrenberg; Franz Hadacek; Ahmad Hama Rashid; Petr Karlovsky. Small-Scale Bioreactor for Sterile Hydroponics and Hairy Roots: Metabolic Diversity and Salicylic Acid Exudation by Hairy Roots of Hyoscyamus niger. Applied Sciences 2019, 9, 3044 .

AMA Style

Zana Jamal Kareem, Ling Su, Anna Rathgeb, Anke Sirrenberg, Franz Hadacek, Ahmad Hama Rashid, Petr Karlovsky. Small-Scale Bioreactor for Sterile Hydroponics and Hairy Roots: Metabolic Diversity and Salicylic Acid Exudation by Hairy Roots of Hyoscyamus niger. Applied Sciences. 2019; 9 (15):3044.

Chicago/Turabian Style

Zana Jamal Kareem; Ling Su; Anna Rathgeb; Anke Sirrenberg; Franz Hadacek; Ahmad Hama Rashid; Petr Karlovsky. 2019. "Small-Scale Bioreactor for Sterile Hydroponics and Hairy Roots: Metabolic Diversity and Salicylic Acid Exudation by Hairy Roots of Hyoscyamus niger." Applied Sciences 9, no. 15: 3044.