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Dr. Hafiz Ahmed
Department of Botany, University of Central Punjab, Punjab Group of Colleges, Bahawalpur 63100, Pakistan

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Research Keywords & Expertise

0 Cell Biology
0 Molecular Genetics
0 Biotechnology Breeding
0 Plant Breeding,
0 Genome-Wide Association Mapping

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Genome-Wide Association Mapping

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Short Biography

Dr. Hafiz Ghulam Muhu-Din Ahmed is working as Head of the Department (HOD), Botany, University of Central Punjab, Punjab Group of Colleges, Bahawalpur-Pakistan. He also served in the Department of Plant Breeding and Genetics, University of Agriculture Faisalabad (sub campus Burewala) for two years and taught to B.Sc (Hons) Agriculture-Plant Breeding and Genetics. He has completed PhD from University of Agriculture Faisalabad, Pakistan in 2018 and selected 10 different drought tolerant wheat genotypes for Pakistan. He has written more than 30 research articles in national and international journals. He has Expertise like, Genome-Wide study in wheat crop, Association mapping, SSR marker, SNPs and GBS studies Molecular Breeding and Expertise in DATA analysis of Major field crops

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Journal article
Published: 18 August 2021 in Agronomy
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Genome-wide association study (GWAS) was performed for stomata- and yield-related attributes with high-density Illumina 90 K Infinium SNP (single nucleotide polymorphism) array in bread wheat to determine genetic potential of germplasm for scarce water resources with sustainable yield potential. Major yield and stomata attributes were phenotyped on a panel of Pakistani and foreign accessions grown in non-stressed and water shortage environments during two seasons. Highly significant variations were shown among accessions in both conditions for examined characteristics. Water shortage conditions reduced the overall wheat yield and strong positive correlation existed among stomatal frequency, leaf venation and grain yield per plant. Population structure analyses based on 90,000 SNP data classified the accessions into four sub-populations which indicated the presence of genetic variability. Marker-trait association (MTA) analyses revealed that 422 significant SNPs at p ≤ 10−3, after crossing the false discovery rate (FDR) <0.05 threshold, were linked with examined attributes. Pleiotropic loci (wsnp_Ex_c8913_14881924 and Tdurum_contig10598_304) were associated with flag leaf area (FLA), stomata size (SS), stomata frequency (SF), leaf venation (LV), number of grain per spike (NGS) and grain yield per plant (GYP), which were located on chromosome 4B and 6B at the positions 173.63cM and 229.64cM, respectively, under water shortage conditions. Pleotropic loci wsnp_Ex_c24167_33416760, wsnp_Ex_c5412_9564046 and Tdurum_contig81797_369 on chromosomes 7A, 2A and 4B at the positions 148.26cM, 261.05cM and 173.63cM, respectively, were significantly linked with stomata and yield indices such as FLA, SS, SF, LV, NGS and GYP under normal and water shortage conditions. The current experiment not only validated several MTAs for studied indices reported in other studies but also discovered novel MTAs significant under water shortage environments. Associated and significant SNPs will be useful in discovering novel genes underpinning water shortage tolerance in bread wheat for producing high-yielding and drought tolerant wheat varieties to fulfill the wheat demand for growing populations.

ACS Style

Hafiz Ghulam Muhu-Din Ahmed; Muhammad Nouman Iqbal; Muhammad Arslan Iqbal; Yawen Zeng; Aziz Ullah; Muhammad Iqbal; Humayun Raza; Muhammad Majid Yar; Nadeem Sarwar; Muhammad Imran; Saddam Hussain. Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions. Agronomy 2021, 11, 1646 .

AMA Style

Hafiz Ghulam Muhu-Din Ahmed, Muhammad Nouman Iqbal, Muhammad Arslan Iqbal, Yawen Zeng, Aziz Ullah, Muhammad Iqbal, Humayun Raza, Muhammad Majid Yar, Nadeem Sarwar, Muhammad Imran, Saddam Hussain. Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions. Agronomy. 2021; 11 (8):1646.

Chicago/Turabian Style

Hafiz Ghulam Muhu-Din Ahmed; Muhammad Nouman Iqbal; Muhammad Arslan Iqbal; Yawen Zeng; Aziz Ullah; Muhammad Iqbal; Humayun Raza; Muhammad Majid Yar; Nadeem Sarwar; Muhammad Imran; Saddam Hussain. 2021. "Genome-Wide Association Mapping for Stomata and Yield Indices in Bread Wheat under Water Limited Conditions." Agronomy 11, no. 8: 1646.

Journal article
Published: 04 September 2020 in Agriculture
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The decrease in water resources is a serious threat to food security world-wide. In this regard, a genome-wide association study (GWAS) was conducted to identify grain yield and quality-related genes/loci under normal and water-deficit conditions. Highly significant differences were exhibited among genotypes under both conditions for all studied traits. Water-deficit stress caused a reduction in grains yield and an increase in grains protein contents (GPC) and gluten contents (GLC). Population structure divided the 96 genotypes into four sub-populations. Out of 72 significant marker-trait associations (MTAs), 28 and 44 were observed under normal and water-deficit stress conditions, respectively. Pleiotropic loci (RAC875_s117925_244, BobWhite_c23828_341 and wsnp_CAP8_c334_304253) for yield and quality traits were identified on chromosomes 5A, 6B and 7B, respectively, under normal conditions. Under a water-deficit condition, the pleiotropic loci (Excalibur_c48047_90, Tdurum_contig100702_265 and BobWhite_c19429_95) for grain yield per plant (GYP), GPC and GLC were identified on chromosomes 3A, 4A and 7B, respectively. The pleiotropic loci (BS00063551_51 and RAC875_c28721_290) for GPC and GLC on chromosome 1B and 3A, respectively, were found under both conditions. Besides the validation of previously reported MTAs, some new MTAs were identified for flag leaf area (FLA), thousand grain weight (TGW), GYP, GPC and GLC under normal and water-deficit conditions. Twenty SNPs associated with the traits were mapped in the coding DNA sequence (CDS) of the respective candidate genes. The protein functions of the identified candidate genes were predicted and discussed. Isolation and characterization of the candidate genes, wherein, SNPs were mapped in CDS will result in discovering novel genes underpinning water-deficit tolerance in bread wheat.

ACS Style

Hafiz Ghulam Muhu-Din Ahmed; Muhammad Sajjad; Yawen Zeng; Muhammad Iqbal; Sultan Habibullah Khan; Aziz Ullah; Malik Nadeem Akhtar. Genome-Wide Association Mapping through 90K SNP Array for Quality and Yield Attributes in Bread Wheat against Water-Deficit Conditions. Agriculture 2020, 10, 392 .

AMA Style

Hafiz Ghulam Muhu-Din Ahmed, Muhammad Sajjad, Yawen Zeng, Muhammad Iqbal, Sultan Habibullah Khan, Aziz Ullah, Malik Nadeem Akhtar. Genome-Wide Association Mapping through 90K SNP Array for Quality and Yield Attributes in Bread Wheat against Water-Deficit Conditions. Agriculture. 2020; 10 (9):392.

Chicago/Turabian Style

Hafiz Ghulam Muhu-Din Ahmed; Muhammad Sajjad; Yawen Zeng; Muhammad Iqbal; Sultan Habibullah Khan; Aziz Ullah; Malik Nadeem Akhtar. 2020. "Genome-Wide Association Mapping through 90K SNP Array for Quality and Yield Attributes in Bread Wheat against Water-Deficit Conditions." Agriculture 10, no. 9: 392.

Journal article
Published: 20 June 2020 in Saudi Journal of Biological Sciences
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The decrease in water resources due to the excessive use of water for irrigation purpose and climatic changes represents a serious world-wide threat to food security. In this regards, 50 wheat accessions were analyzed, using completely random factorial design at the seedlings stage under normal and drought stress conditions. Significant variation was detected among all accessions under both conditions. All characters studied showed variations in the mean values in water deficit environments in studied gemplasm at seedling stage. As seedling fresh weight, dry weight, relative water content, cell membrane thermo-stability, chlorophyll a & b were positively associated among themselves under drought conditions which showed the significance of these attribute for water deficit areas in future wheat breeding programs. Based on their performance, five accessions namely Aas-11, Chakwal-86, Pasban-90, Chakwal-97 and Kohistan-97 were selected as drought tolerant and three accessions namely Mairaj-08, Lasani-2008 and Gomal-2008 were selected as drought susceptible genotypes. The choice of wheat accessions based on the characteristics of the seedlings is informal, low-priced and less hassle. Likewise, the seedlings attributes exhibit moderate to high variation with an additive genetics effects on the environments. Best performance accessions under water deficit environment will be beneficial in future wheat breeding schemes and early screening for the attributes suggested in current experiment will be useful for producing best-yielded and drought-tolerance wheat genotypes to sustainable food security.

ACS Style

Hafiz Ghulam Muhu-Din Ahmed; Yawen Zeng; Xiaomeng Yang; Hafiz Arslan Anwaar; Muhammad Zeeshan Mansha; Ch. M. Shahid Hanif; Kamran Ikram; Aziz Ullah; Suliman Mohammed Suliman Alghanem. Conferring drought-tolerant wheat genotypes through morpho-physiological and chlorophyll indices at seedling stage. Saudi Journal of Biological Sciences 2020, 27, 2116 -2123.

AMA Style

Hafiz Ghulam Muhu-Din Ahmed, Yawen Zeng, Xiaomeng Yang, Hafiz Arslan Anwaar, Muhammad Zeeshan Mansha, Ch. M. Shahid Hanif, Kamran Ikram, Aziz Ullah, Suliman Mohammed Suliman Alghanem. Conferring drought-tolerant wheat genotypes through morpho-physiological and chlorophyll indices at seedling stage. Saudi Journal of Biological Sciences. 2020; 27 (8):2116-2123.

Chicago/Turabian Style

Hafiz Ghulam Muhu-Din Ahmed; Yawen Zeng; Xiaomeng Yang; Hafiz Arslan Anwaar; Muhammad Zeeshan Mansha; Ch. M. Shahid Hanif; Kamran Ikram; Aziz Ullah; Suliman Mohammed Suliman Alghanem. 2020. "Conferring drought-tolerant wheat genotypes through morpho-physiological and chlorophyll indices at seedling stage." Saudi Journal of Biological Sciences 27, no. 8: 2116-2123.

Journal article
Published: 01 May 2020
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ACS Style

Hafiz Gmd Ahmed. Genome Wide Diversity in Bread Wheat Evaluated by SSR Markers. 2020, 24, 263 -272.

AMA Style

Hafiz Gmd Ahmed. Genome Wide Diversity in Bread Wheat Evaluated by SSR Markers. . 2020; 24 (2):263-272.

Chicago/Turabian Style

Hafiz Gmd Ahmed. 2020. "Genome Wide Diversity in Bread Wheat Evaluated by SSR Markers." 24, no. 2: 263-272.

Journal article
Published: 15 April 2020 in World Journal of Biology and Biotechnology
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Ribonucleic acid (RNA) silencing, RNA interference (RNAi) or post-transcriptional gene silencing takes place in a variety of eukaryotes and it was discovered firstly in the plants. The RNA silencing process is activated by a trigger from dsRNA predecessor. A very important step in the silencing pathways the conversion of dsRNA into small duplexes of RNA of the representative length and arrangement. Then these small dsRNA monitor RNA silencing by different mechanisms. Post transcriptional gene silencing mechanisms were initially identified as an anti-viral process that give protection to the organisms from the viruses or which inhibit the unsystematic incorporation of transposable components. The basic aim of this review article is to study the mechanism of gene silencing by dsRNA and the roles of certain proteins in cellular post transcriptional RNA silencing machinery and finally we also discuss the RNA silencing as an anti-viral defense mechanism in the plants.

ACS Style

Hafiz Gmd Ahmed; Shadab Shaukat; Iqra Kousar; Maria Rafiq; Maria Ilyas; Aziz Ullah; Khuda Buksh. Gene silencing by double stranded Ribonucleic acid (RNA). World Journal of Biology and Biotechnology 2020, 5, 41 -44.

AMA Style

Hafiz Gmd Ahmed, Shadab Shaukat, Iqra Kousar, Maria Rafiq, Maria Ilyas, Aziz Ullah, Khuda Buksh. Gene silencing by double stranded Ribonucleic acid (RNA). World Journal of Biology and Biotechnology. 2020; 5 (1):41-44.

Chicago/Turabian Style

Hafiz Gmd Ahmed; Shadab Shaukat; Iqra Kousar; Maria Rafiq; Maria Ilyas; Aziz Ullah; Khuda Buksh. 2020. "Gene silencing by double stranded Ribonucleic acid (RNA)." World Journal of Biology and Biotechnology 5, no. 1: 41-44.

Journal article
Published: 05 November 2019 in Journal of Integrative Agriculture
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Genetic diversity is the base of any genetic improvement breeding program aimed at stress breeding. The variability among breeding materials is of primary importance in the achievements of a good crop production. Herein, 105 wheat genotypes were screened against drought stress using factorial completely randomized design at seedling stage to determine the genetic diversity and traits association conferring drought tolerance. Analysis of variances revealed that all the studied parameters differed significantly among all genotypes, indicating the significance genetic variability existed among all genotypes for studied indices. The 10 best performance genotypes G1, G6, G11, G16, G21, G26, G39, G44, G51, and G61 were screened as drought tolerant, while five lowest performance genotypes G3, G77, G91, G98, and G105 were screened as drought susceptible. Root length, chlorophyll a, chlorophyll b, and carotenoid contents were significantly correlated among themselves which exhibited the importance of these indices for rainfed areas in future wheat breeding scheme. Shoot length exhibited non-significant and negative association with other studied traits, and its selection seems not to be a promising criteria for this germplasm for drought stress. Best performance genotypes under drought stress conditions will be useful in future wheat breeding program and early selection will be effective for developing high yielding and drought tolerant wheat varieties.

ACS Style

Hafiz Ghulam Muhu-Din Ahmed; Abdus Salam Khan; Ming-Ju Li; Sultan Habibullah Khan; Muhammad Kashif. Early selection of bread wheat genotypes using morphological and photosynthetic attributes conferring drought tolerance. Journal of Integrative Agriculture 2019, 18, 2483 -2491.

AMA Style

Hafiz Ghulam Muhu-Din Ahmed, Abdus Salam Khan, Ming-Ju Li, Sultan Habibullah Khan, Muhammad Kashif. Early selection of bread wheat genotypes using morphological and photosynthetic attributes conferring drought tolerance. Journal of Integrative Agriculture. 2019; 18 (11):2483-2491.

Chicago/Turabian Style

Hafiz Ghulam Muhu-Din Ahmed; Abdus Salam Khan; Ming-Ju Li; Sultan Habibullah Khan; Muhammad Kashif. 2019. "Early selection of bread wheat genotypes using morphological and photosynthetic attributes conferring drought tolerance." Journal of Integrative Agriculture 18, no. 11: 2483-2491.

Journal article
Published: 05 May 2019 in Sustainability
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Diminishing water resources as a result of excessive use of water for irrigation and climate change posture a severe global threat to food security. Herein, an experiment was conducted to determine the selection criteria for drought-tolerant bread wheat genotypes at the seedling stage using morphological and photosynthetic pigmentation-related traits. A panel of 105 wheat landraces, historical Pakistani varieties, and advance breeding lines were evaluated under normal and drought stress using factorial completely randomized design. The root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b were positively correlated among themselves under both normal and stress conditions. Hence, selection of any one of these traits enhances the performance of other traits. The shoot length was non-significant and negatively associated with all other studied characters except relative water content. The results suggested that selection for shoot length could not improve genetic gain for drought tolerance. Out of 10 principal components (PCs), the first three PCs were showed significant genetic variation under both conditions. The first three PCs showed 74.6% and 76% cumulative genetic variation under normal and drought conditions, respectively. Based on PCA, 10 drought-tolerant and five drought-susceptible genotypes were identified. Overall results suggested that selection for root length, fresh weight, dry weight, cell membrane thermo-stability, and chlorophyll b at the seedling stage would improve genetic gain for drought tolerance. The outperforming genotypes under drought stress conditions can be useful in future wheat breeding programs, and early selection for the traits recommended in this study will be effective for developing high-yielding and drought-tolerant wheat varieties.

ACS Style

Hafiz Ahmed; Muhammad Sajjad; Mingju Li; Muhammad Azmat; Muhammad Rizwan; Rana Maqsood; Sultan Khan. Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage. Sustainability 2019, 11, 2584 .

AMA Style

Hafiz Ahmed, Muhammad Sajjad, Mingju Li, Muhammad Azmat, Muhammad Rizwan, Rana Maqsood, Sultan Khan. Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage. Sustainability. 2019; 11 (9):2584.

Chicago/Turabian Style

Hafiz Ahmed; Muhammad Sajjad; Mingju Li; Muhammad Azmat; Muhammad Rizwan; Rana Maqsood; Sultan Khan. 2019. "Selection Criteria for Drought-Tolerant Bread Wheat Genotypes at Seedling Stage." Sustainability 11, no. 9: 2584.

Journal article
Published: 28 March 2018 in Journal of the National Science Foundation of Sri Lanka
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ACS Style

Hafiz Ghulam Muhu-Din Ahmed; Abdus Salam Khan; Muhammad Kashif; Sultan Habibullah Khan. Genetic analysis of yield and physical traits of spring wheat grain. Journal of the National Science Foundation of Sri Lanka 2018, 46, 23 .

AMA Style

Hafiz Ghulam Muhu-Din Ahmed, Abdus Salam Khan, Muhammad Kashif, Sultan Habibullah Khan. Genetic analysis of yield and physical traits of spring wheat grain. Journal of the National Science Foundation of Sri Lanka. 2018; 46 (1):23.

Chicago/Turabian Style

Hafiz Ghulam Muhu-Din Ahmed; Abdus Salam Khan; Muhammad Kashif; Sultan Habibullah Khan. 2018. "Genetic analysis of yield and physical traits of spring wheat grain." Journal of the National Science Foundation of Sri Lanka 46, no. 1: 23.

Journal article
Published: 12 May 2017 in Zemdirbyste-Agriculture
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ACS Style

Aamer Mumtaz; Hafeez Ahmad Sadaqat; Muhammad Saeed; Muhammad Irfan Yousaf; Aamar Shehzad; Hafiz Ghulam Moh Din Ahmed. Genetic behaviour of qualitative and seed yield-related traits in Brassica rapa Aliejinio rapso kokybiniai ir su sėklų derliumi susiję genetiniai požymiai. Zemdirbyste-Agriculture 2017, 104, 147 -156.

AMA Style

Aamer Mumtaz, Hafeez Ahmad Sadaqat, Muhammad Saeed, Muhammad Irfan Yousaf, Aamar Shehzad, Hafiz Ghulam Moh Din Ahmed. Genetic behaviour of qualitative and seed yield-related traits in Brassica rapa Aliejinio rapso kokybiniai ir su sėklų derliumi susiję genetiniai požymiai. Zemdirbyste-Agriculture. 2017; 104 (2):147-156.

Chicago/Turabian Style

Aamer Mumtaz; Hafeez Ahmad Sadaqat; Muhammad Saeed; Muhammad Irfan Yousaf; Aamar Shehzad; Hafiz Ghulam Moh Din Ahmed. 2017. "Genetic behaviour of qualitative and seed yield-related traits in Brassica rapa Aliejinio rapso kokybiniai ir su sėklų derliumi susiję genetiniai požymiai." Zemdirbyste-Agriculture 104, no. 2: 147-156.

Journal article
Published: 10 February 2016 in Plant Omics
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ACS Style

Syed Adeel Zafar; Muzammil Hussain; Mubashar Raza; Hafiz Gmd Ahmed; Iqrar Ahmad Rana; Bushra Sadia; Rana Muhammad Atif. Genome wide analysis of heat shock transcription factor (HSF) family in chickpea and its comparison with Arabidopsis. Plant Omics 2016, 9, 136 -141.

AMA Style

Syed Adeel Zafar, Muzammil Hussain, Mubashar Raza, Hafiz Gmd Ahmed, Iqrar Ahmad Rana, Bushra Sadia, Rana Muhammad Atif. Genome wide analysis of heat shock transcription factor (HSF) family in chickpea and its comparison with Arabidopsis. Plant Omics. 2016; 9 (2):136-141.

Chicago/Turabian Style

Syed Adeel Zafar; Muzammil Hussain; Mubashar Raza; Hafiz Gmd Ahmed; Iqrar Ahmad Rana; Bushra Sadia; Rana Muhammad Atif. 2016. "Genome wide analysis of heat shock transcription factor (HSF) family in chickpea and its comparison with Arabidopsis." Plant Omics 9, no. 2: 136-141.