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Dr. Biancamaria Ciasca
ISPA-CNR Bari, Italy

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0 Food Chemistry
0 Mycotoxins
0 Food analysis
0 Mass spectometry
0 Food chemistry, metabolomics

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Journal article
Published: 10 December 2020 in Toxins
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An analytical method for the simultaneous determination of trichothecenes—namely, nivalenol (NIV), deoxynivalenol (DON) and its acetylated derivatives (3- and 15-acetyl-DON), T-2 and HT-2 toxins—and zearalenone (ZEN) in wheat, wheat flour, and wheat crackers was validated through a collaborative study involving 15 participants from 10 countries. The validation study, performed within the M/520 standardization mandate of the European Commission, was carried out according to the IUPAC (International Union of Pure and Applied Chemistry) International Harmonized Protocol. The method was based on mycotoxin extraction from the homogenized sample material with a mixture of acetonitrile-water followed by purification and concentration on a solid phase extraction column. High-performance liquid chromatography coupled with tandem mass spectrometry was used for mycotoxin detection, using isotopically labelled mycotoxins as internal standards. The tested contamination ranges were from 27.7 to 378 μg/kg for NIV, from 234 to 2420 μg/kg for DON, from 18.5 to 137 μg/kg for 3-acetyl-DON, from 11.4 to 142 μg/kg for 15-acetyl-DON, from 2.1 to 37.6 μg/kg for T-2 toxin, from 6.6 to 134 μg/kg for HT-2 toxin, and from 31.6 to 230 μg/kg for ZEN. Recoveries were in the range 71–97% with the lowest values for NIV, the most polar mycotoxin. The relative standard deviation for repeatability (RSDr) was in the range of 2.2–34%, while the relative standard deviation for reproducibility (RSDR) was between 6.4% and 45%. The HorRat values ranged from 0.4 to 2.0. The results of the collaborative study showed that the candidate method is fit for the purpose of enforcing the legislative limits of the major Fusarium toxins in wheat and wheat-based products.

ACS Style

Annalisa De Girolamo; Biancamaria Ciasca; Michelangelo Pascale; Veronica M.T. Lattanzio. Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study. Toxins 2020, 12, 786 .

AMA Style

Annalisa De Girolamo, Biancamaria Ciasca, Michelangelo Pascale, Veronica M.T. Lattanzio. Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study. Toxins. 2020; 12 (12):786.

Chicago/Turabian Style

Annalisa De Girolamo; Biancamaria Ciasca; Michelangelo Pascale; Veronica M.T. Lattanzio. 2020. "Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study." Toxins 12, no. 12: 786.

Methods article
Published: 05 June 2020 in Frontiers in Plant Science
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Liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS) represents the most powerful metabolomics platform to investigate biological systems. Reproducible and standardized workflows allow obtaining a meaningful biological interpretation. The purpose of this study was to set up and apply an open-source workflow for LC-HRMS plant metabolomics studies. Key steps of the proposed workflow were as follows: (1) experimental design, (2) sample preparation, (3) LC-HRMS analysis, (4) data processing, (5) custom database search, (6) statistical analysis, (7) compound identification, and (8) biochemical interpretation. Its applicability was evaluated through the study of metabolomics changes of two maize recombinant inbred lines with contrasting phenotypes with respect to disease severity after Fusarium verticillioides infection of seedlings. Analysis of data from the case-control study revealed abundance change in metabolites belonging to different metabolic pathways, including two amino acids (L-tryptophan and tyrosine), five flavonoids, and three N-hydroxynnamic acid amides.

ACS Style

Biancamaria Ciasca; Alessandra Lanubile; Adriano Marocco; Michelangelo Pascale; Antonio F. Logrieco; Veronica M. T. Lattanzio. Application of an Integrated and Open Source Workflow for LC-HRMS Plant Metabolomics Studies. Case-Control Study: Metabolic Changes of Maize in Response to Fusarium verticillioides Infection. Frontiers in Plant Science 2020, 11, 1 .

AMA Style

Biancamaria Ciasca, Alessandra Lanubile, Adriano Marocco, Michelangelo Pascale, Antonio F. Logrieco, Veronica M. T. Lattanzio. Application of an Integrated and Open Source Workflow for LC-HRMS Plant Metabolomics Studies. Case-Control Study: Metabolic Changes of Maize in Response to Fusarium verticillioides Infection. Frontiers in Plant Science. 2020; 11 ():1.

Chicago/Turabian Style

Biancamaria Ciasca; Alessandra Lanubile; Adriano Marocco; Michelangelo Pascale; Antonio F. Logrieco; Veronica M. T. Lattanzio. 2020. "Application of an Integrated and Open Source Workflow for LC-HRMS Plant Metabolomics Studies. Case-Control Study: Metabolic Changes of Maize in Response to Fusarium verticillioides Infection." Frontiers in Plant Science 11, no. : 1.

Journal article
Published: 22 April 2020 in Toxins
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Aflatoxin B1 (AFB1) is a secondary metabolite produced by some Aspergillus spp. fungi affecting many crops and feed materials. Aflatoxin M1 (AFM1), the 4-hydroxylated metabolite of AFB1, is the main AFB1-related compound present in milk, and it is categorized by the International Agency for Research on Cancer (IARC) as a “group 1 human carcinogen”. The aim of this work was to evaluate and compare the analytical performances of two commercial immunoassays widely applied for the detection of AFM1 in milk, namely strip test immunoassay and enzyme linked immunosorbent assay (ELISA). Assay validation included samples at AFM1 levels of 25, 50, 75 ng/kg and blank samples (AFM1 < 0.5 ng/kg). With respect to a screening target concentration (STC) of 50 ng/kg the two assays showed cut-off values of 37.7 ng/kg and 47.5 ng/kg for strip test and ELISA, respectively, a false suspect rate for blanks

ACS Style

Ivan Pecorelli; Natascia Guarducci; Cristoph Von Holst; Rita Bibi; Michelangelo Pascale; Biancamaria Ciasca; Antonio F. Logrieco; Veronica M. T. Lattanzio. Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk. Toxins 2020, 12, 270 .

AMA Style

Ivan Pecorelli, Natascia Guarducci, Cristoph Von Holst, Rita Bibi, Michelangelo Pascale, Biancamaria Ciasca, Antonio F. Logrieco, Veronica M. T. Lattanzio. Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk. Toxins. 2020; 12 (4):270.

Chicago/Turabian Style

Ivan Pecorelli; Natascia Guarducci; Cristoph Von Holst; Rita Bibi; Michelangelo Pascale; Biancamaria Ciasca; Antonio F. Logrieco; Veronica M. T. Lattanzio. 2020. "Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk." Toxins 12, no. 4: 270.

Journal article
Published: 11 February 2020 in Microorganisms
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Investigating the in vitro fumonisin biosynthesis and the genetic structure of Fusarium verticillioides populations can provide important insights into the relationships between strains originating from various world regions. In this study, 90 F. verticillioides strains isolated from maize in five Mediterranean countries (Italy, Spain, Tunisia, Egypt and Iran) were analyzed to investigate their ability to in vitro biosynthesize fumonisin B1, fumonisin B2 and fumonisin B3 and to characterize their genetic profile. In general, 80% of the analyzed strains were able to biosynthesize fumonisins (range 0.03–69.84 μg/g). Populations from Italy, Spain, Tunisia and Iran showed a similar percentage of fumonisin producing strains (>90%); conversely, the Egyptian population showed a lower level of producing strains (46%). Significant differences in fumonisin biosynthesis were detected among strains isolated in the same country and among strains isolated from different countries. A portion of the divergent FUM1 gene and of intergenic regions FUM6-FUM7 and FUM7-FUM8 were sequenced to evaluate strain diversity among populations. A high level of genetic uniformity inside the populations analyzed was detected. Apparently, neither geographical origin nor fumonisin production ability were correlated to the genetic diversity of the strain set. However, four strains from Egypt differed from the remaining strains.

ACS Style

Giovanni Beccari; Łukasz Stępień; Andrea Onofri; Veronica M. T. Lattanzio; Biancamaria Ciasca; Sally I. Abd-El Fatah; Francesco Valente; Monika Urbaniak; Lorenzo Covarelli. In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries. Microorganisms 2020, 8, 241 .

AMA Style

Giovanni Beccari, Łukasz Stępień, Andrea Onofri, Veronica M. T. Lattanzio, Biancamaria Ciasca, Sally I. Abd-El Fatah, Francesco Valente, Monika Urbaniak, Lorenzo Covarelli. In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries. Microorganisms. 2020; 8 (2):241.

Chicago/Turabian Style

Giovanni Beccari; Łukasz Stępień; Andrea Onofri; Veronica M. T. Lattanzio; Biancamaria Ciasca; Sally I. Abd-El Fatah; Francesco Valente; Monika Urbaniak; Lorenzo Covarelli. 2020. "In Vitro Fumonisin Biosynthesis and Genetic Structure of Fusarium verticillioides Strains from Five Mediterranean Countries." Microorganisms 8, no. 2: 241.

Journal article
Published: 06 November 2019 in Food Chemistry
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A flow injection – mass spectrometry method for rapid glyphosate detection in food commodities was developed and validated. The sample preparation protocol included a simple and rapid extract purification step through polymeric solid phase extraction cartridges followed by addition of isotopically labeled glyphosate to the final test sample. The optimized method was subjected to intra-laboratory validation (spiking range 0.5–100 mg/kg) in chickpeas, grapes and apples, as representatives of three different commodity groups as defined in SANTE/11813/2017 guidelines. Recoveries were in the range 60–111%, repeatability and within laboratory reproducibility were ≤17%.The trueness of the results generated with the developed method was evaluated by analysis of a set of incurred chickpea and wheat samples (glyphosate range 0.5–36 mg/kg) and comparison with the reference method (Quick Polar Pesticides Method), confirming the method fitness-for-purpose of rapid compliance testing.

ACS Style

Biancamaria Ciasca; Ivan Pecorelli; Lavinia Lepore; Angela Paoloni; Lucia Catucci; Michelangelo Pascale; Veronica Maria Teresa Lattanzio. Rapid and reliable detection of glyphosate in pome fruits, berries, pulses and cereals by flow injection – Mass spectrometry. Food Chemistry 2019, 310, 125813 .

AMA Style

Biancamaria Ciasca, Ivan Pecorelli, Lavinia Lepore, Angela Paoloni, Lucia Catucci, Michelangelo Pascale, Veronica Maria Teresa Lattanzio. Rapid and reliable detection of glyphosate in pome fruits, berries, pulses and cereals by flow injection – Mass spectrometry. Food Chemistry. 2019; 310 ():125813.

Chicago/Turabian Style

Biancamaria Ciasca; Ivan Pecorelli; Lavinia Lepore; Angela Paoloni; Lucia Catucci; Michelangelo Pascale; Veronica Maria Teresa Lattanzio. 2019. "Rapid and reliable detection of glyphosate in pome fruits, berries, pulses and cereals by flow injection – Mass spectrometry." Food Chemistry 310, no. : 125813.

Journal article
Published: 01 November 2019 in Applied Clay Science
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ACS Style

Ignazio Allegretta; Biancamaria Ciasca; Maria D.R. Pizzigallo; Veronica M.T. Lattanzio; Roberto Terzano. A fast method for the chemical analysis of clays by total-reflection x-ray fluorescence spectroscopy (TXRF). Applied Clay Science 2019, 180, 1 .

AMA Style

Ignazio Allegretta, Biancamaria Ciasca, Maria D.R. Pizzigallo, Veronica M.T. Lattanzio, Roberto Terzano. A fast method for the chemical analysis of clays by total-reflection x-ray fluorescence spectroscopy (TXRF). Applied Clay Science. 2019; 180 ():1.

Chicago/Turabian Style

Ignazio Allegretta; Biancamaria Ciasca; Maria D.R. Pizzigallo; Veronica M.T. Lattanzio; Roberto Terzano. 2019. "A fast method for the chemical analysis of clays by total-reflection x-ray fluorescence spectroscopy (TXRF)." Applied Clay Science 180, no. : 1.

Journal article
Published: 01 July 2019 in Toxins
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T-2 and HT-2 toxins and their main modified forms (T-2 glucoside and HT-2 glucoside) may co-occur in cereals and cereal-based products. A fluorescence polarization immunoassay (FPIA) was developed for the simultaneous determination of T-2 toxin, HT-2 toxin and relevant glucosides, expressed as sum. The developed FPIA, using a HT-2-specific antibody, showed high sensitivity (IC50 = 2.0 ng/mL) and high cross-reactivity (100% for T-2 toxin and 80% for T-2 and HT-2 glucosides). The FPIA has been used to develop two rapid and easy-to-use methods using two different extraction protocols, based on the use of organic (methanol/water, 90:10, v/v) and non-organic (water) solvents, for the determination of these toxins in wheat. The two proposed methods showed analytical performances in terms of sensitivity (LOD 10 µg/kg) recovery (92-97%) and precision (relative standard deviations ≤13%), fulfilling the criteria for acceptability of an analytical method for the quantitative determination of T-2 and HT-2 toxins established by the European Union. Furthermore, the methods were then validated in accordance with the harmonized guidelines for the validation of screening methods included in the Regulation (EU) No. 519/2014. The satisfactory analytical performances, in terms of intermediate precision (≤25%), cut-off level (80 and 96 µg/kg for the two methods) and rate of false positives (<0.1%) confirmed the applicability of the proposed methods as screening method for assessing the content of these toxins in wheat at the EU indicative levels reported for T-2 and HT-2 toxins.

ACS Style

Vincenzo Lippolis; Anna C. R. Porricelli; Erminia Mancini; Biancamaria Ciasca; Veronica M. T. Lattanzio; Annalisa De Girolamo; Chris M. Maragos; Susan McCormick; Peiwu Li; Antonio F. Logrieco; Michelangelo Pascale. Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat. Toxins 2019, 11, 380 .

AMA Style

Vincenzo Lippolis, Anna C. R. Porricelli, Erminia Mancini, Biancamaria Ciasca, Veronica M. T. Lattanzio, Annalisa De Girolamo, Chris M. Maragos, Susan McCormick, Peiwu Li, Antonio F. Logrieco, Michelangelo Pascale. Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat. Toxins. 2019; 11 (7):380.

Chicago/Turabian Style

Vincenzo Lippolis; Anna C. R. Porricelli; Erminia Mancini; Biancamaria Ciasca; Veronica M. T. Lattanzio; Annalisa De Girolamo; Chris M. Maragos; Susan McCormick; Peiwu Li; Antonio F. Logrieco; Michelangelo Pascale. 2019. "Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat." Toxins 11, no. 7: 380.

Special issue article
Published: 10 July 2018 in Journal of Mass Spectrometry
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A strong trend toward using highly selective mass spectrometry technologies for screening of multiple mycotoxins has been observed in recent years. In the present study, the process of validation of a multi‐mycotoxin screening method based on liquid chromatography high‐resolution mass spectrometry method is presented. The method was intended for the simultaneous screening of the major Fusarium toxins (deoxynivalenol, 3‐ and 15‐acetyl deoxynivalenol, T‐2 and HT‐2 toxins, zearalenone, enniatins A, A1, B, B1, beauvericin) in wheat. The sample preparation protocol was based on a double extraction (methanol followed by acetonitrile/water mixture), and purification through solid phase extraction C18 column. To provide insights for full exploitation of the potential of the double stage HRMS detection a full scan acquisition event followed by a sequence of five fragmentation events (vDIA) were set for mycotoxin detection, the latter to be exploited for confirmatory purposes. Method analytical performances were evaluated through in house validation and small scale interlaboratory study, designed according to Commission Regulation 519/2014/EU, setting performance requirements for screening methods for mycotoxins. Screening target concentrations (STC) were close to EU maximum permitted or indicative levels. The in house validation provided the precision of the response under repeatability conditions and the intermediate precision (both resulting lower than 30%), the cut off value, and the rate of false suspect results for negative (free of the mycotoxin of interest) samples, that resulted lower than 0.1% in all cases. The collaborative study provided reproducibility and laboratory independent cut off values. Analysis of reference materials proved method trueness and suitability for screening of the major Fusarium mycotoxins in wheat. Finally the applicability of the full scan/vDIA detection approach was successfully tested on a set of naturally contaminated wheat samples, where two characteristic product ions could be detected for all identified mycotoxins even at levels in the low μg/kg range.

ACS Style

Biancamaria Ciasca; Michelangelo Pascale; Valerio Guido Altieri; Francesco Longobardi; Michele Suman; Dante Catellani; Veronica M.T. Lattanzio. In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat. Journal of Mass Spectrometry 2018, 53, 743 -752.

AMA Style

Biancamaria Ciasca, Michelangelo Pascale, Valerio Guido Altieri, Francesco Longobardi, Michele Suman, Dante Catellani, Veronica M.T. Lattanzio. In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat. Journal of Mass Spectrometry. 2018; 53 (9):743-752.

Chicago/Turabian Style

Biancamaria Ciasca; Michelangelo Pascale; Valerio Guido Altieri; Francesco Longobardi; Michele Suman; Dante Catellani; Veronica M.T. Lattanzio. 2018. "In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat." Journal of Mass Spectrometry 53, no. 9: 743-752.

Journals
Published: 27 November 2017 in Analytical Methods
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The analytical performances of a lateral flow immunoassay (AFLA-V AQUA™, Vicam a Waters Business) for the determination of aflatoxins in maize were evaluated according to Commission Regulation (EU) No. 519/2014.

ACS Style

Veronica M. T. Lattanzio; Natascia Guarducci; Stephen Powers; Biancamaria Ciasca; Michelangelo Pascale; Christoph von Holst. Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction. Analytical Methods 2017, 10, 123 -130.

AMA Style

Veronica M. T. Lattanzio, Natascia Guarducci, Stephen Powers, Biancamaria Ciasca, Michelangelo Pascale, Christoph von Holst. Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction. Analytical Methods. 2017; 10 (1):123-130.

Chicago/Turabian Style

Veronica M. T. Lattanzio; Natascia Guarducci; Stephen Powers; Biancamaria Ciasca; Michelangelo Pascale; Christoph von Holst. 2017. "Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction." Analytical Methods 10, no. 1: 123-130.

Journal article
Published: 01 September 2017 in Food Chemistry
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The occurrence of deoxynivalenol, 3- and 15-deoxynivalenol and deoxynivalenol-3-glucoside in 84 durum wheat samples, from the Argentinean main growing area, was investigated during 2012/13 and 2013/14 using LC-MS/MS. Deoxynivalenol was found in all samples at concentrations varying between

ACS Style

Sofía A. Palacios; Jessica Gabriela Erazo; Biancamaria Ciasca; Veronica M.T. Lattanzio; María M. Reynoso; María C. Farnochi; Adriana M. Torres. Occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in durum wheat from Argentina. Food Chemistry 2017, 230, 728 -734.

AMA Style

Sofía A. Palacios, Jessica Gabriela Erazo, Biancamaria Ciasca, Veronica M.T. Lattanzio, María M. Reynoso, María C. Farnochi, Adriana M. Torres. Occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in durum wheat from Argentina. Food Chemistry. 2017; 230 ():728-734.

Chicago/Turabian Style

Sofía A. Palacios; Jessica Gabriela Erazo; Biancamaria Ciasca; Veronica M.T. Lattanzio; María M. Reynoso; María C. Farnochi; Adriana M. Torres. 2017. "Occurrence of deoxynivalenol and deoxynivalenol-3-glucoside in durum wheat from Argentina." Food Chemistry 230, no. : 728-734.

Review article
Published: 17 November 2016 in TrAC Trends in Analytical Chemistry
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Two Proficiency Testings (PTs) involving eighteen laboratory participants from 10 Countries have been conducted in 2014 for the simultaneous determination of deoxynivalenol, fumonisins B1 and B2, zearalenone, T-2 and HT-2 toxins, ochratoxin A and aflatoxins B1, B2, G1 and G2 in maize and of deoxynivalenol, zearalenone, T-2 and HT-2 toxins and ochratoxin A in wheat, respectively. The aim of PTs was to check next to the laboratory performance the state-of-art of the LC–MS multi-mycotoxin methods used by participants. In addition, the trend of performances of LC–MS methods for multi-mycotoxin determination in maize together with method-related issues was assessed by comparing three PTs organised over the years 2011–2014. Data showed the improvement of laboratory performances with the overall acceptable z-scores that progressively increased from 59% in 2011 PT to 85% in 2014 PT, while the rate of unacceptable z-score decreased from 25% in 2011 PT to 11% in 2014 PT.

ACS Style

Annalisa De Girolamo; Biancamaria Ciasca; Joerg Stroka; Stefanka Bratinova; Michelangelo Pascale; Angelo Visconti; Veronica M.T. Lattanzio. Performance evaluation of LC–MS/MS methods for multi-mycotoxin determination in maize and wheat by means of international Proficiency Testing. TrAC Trends in Analytical Chemistry 2016, 86, 222 -234.

AMA Style

Annalisa De Girolamo, Biancamaria Ciasca, Joerg Stroka, Stefanka Bratinova, Michelangelo Pascale, Angelo Visconti, Veronica M.T. Lattanzio. Performance evaluation of LC–MS/MS methods for multi-mycotoxin determination in maize and wheat by means of international Proficiency Testing. TrAC Trends in Analytical Chemistry. 2016; 86 ():222-234.

Chicago/Turabian Style

Annalisa De Girolamo; Biancamaria Ciasca; Joerg Stroka; Stefanka Bratinova; Michelangelo Pascale; Angelo Visconti; Veronica M.T. Lattanzio. 2016. "Performance evaluation of LC–MS/MS methods for multi-mycotoxin determination in maize and wheat by means of international Proficiency Testing." TrAC Trends in Analytical Chemistry 86, no. : 222-234.

Research article
Published: 11 July 2016 in Journal of Agricultural and Food Chemistry
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In this study, the naturally debittered table olives cv Bella di Cerignola were studied in order to (i) characterize their phenolic composition; (ii) evaluate the polyphenols bioaccessibility; (iii) assess their absorption and transport, across Caco2/TC7. LC-MS/MS analysis has confirmed the presence of hydroxytyrosol acetate, caffeoyl-6′-secologanoside, and comselogoside. In vitro bioaccessibility ranged from 7% of luteolin to 100% of tyrosol, highlighting the flavonoids sensitivity to the digestive conditions. The Caco2/TC7 polyphenols accumulation was rapid (60 min) with an efficiency of 0.89%; the overall bioavailability was 1.86% (120 min), with hydroxytyrosol and tyrosol the highest bioavailables, followed by verbascoside and luteolin. In the cells and basolateral side, caffeic and coumaric acids metabolites, probably derived from esterase activities, were detected. In conclusion, the naturally debittered table olives cv Bella di Cerignola can be considered as a source of bioaccessible, absorbable, and bioavailable polyphenols that, for their potential health promoting effect, permit inclusion of table olives as a functional food suitable for a balanced diet.

ACS Style

Isabella D’Antuono; Antonella Garbetta; Biancamaria Ciasca; Vito Linsalata; Fiorenza Minervini; Veronica M. T. Lattanzio; Antonio F. Logrieco; Angela Cardinali. Biophenols from Table Olive cv Bella di Cerignola: Chemical Characterization, Bioaccessibility, and Intestinal Absorption. Journal of Agricultural and Food Chemistry 2016, 64, 5671 -5678.

AMA Style

Isabella D’Antuono, Antonella Garbetta, Biancamaria Ciasca, Vito Linsalata, Fiorenza Minervini, Veronica M. T. Lattanzio, Antonio F. Logrieco, Angela Cardinali. Biophenols from Table Olive cv Bella di Cerignola: Chemical Characterization, Bioaccessibility, and Intestinal Absorption. Journal of Agricultural and Food Chemistry. 2016; 64 (28):5671-5678.

Chicago/Turabian Style

Isabella D’Antuono; Antonella Garbetta; Biancamaria Ciasca; Vito Linsalata; Fiorenza Minervini; Veronica M. T. Lattanzio; Antonio F. Logrieco; Angela Cardinali. 2016. "Biophenols from Table Olive cv Bella di Cerignola: Chemical Characterization, Bioaccessibility, and Intestinal Absorption." Journal of Agricultural and Food Chemistry 64, no. 28: 5671-5678.

Journal article
Published: 09 February 2016 in Pest Management Science
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Seed germination is a key phase of the parasitic plant life cycle that is stimulated by the secondary metabolites, mainly strigolactones (SLs), secreted by the host roots. Interventions during this stage would be particularly suitable for parasitic weed management practices, as blocking these chemical signals would prevent seed germination and thus parasite attack. Four fungal strains with different ecological functions were considered for their possible ability to metabolise SLs: Fusarium oxysporum and F. solani, biocontrol agents of Phelipanche ramosa; Trichoderma harzianum, a potential biopesticide; Botrytis cinerea, a phytopathogenic fungus. Four different SLs [the natural strigol, 5-deoxystrigol (5DS) and 4-deoxyorobanchol (4DO), and the synthetic analogue GR24] were added to fungal cultures, followed by determination of the SL content by liquid chromatography–tandem mass spectrometry. Differences were observed among microorganisms, treatments and SLs used. T. harzianum and F. oxysporum were the most capable of reducing the SL content; considering the whole set of fungi used, 5DS and 4DO proved to be the most degradable SLs. Beneficial microscopic fungi could differently be used for biocontrolling parasitic weeds, acting as a ‘physiological’ barrier, by preventing the germination of their seeds through the ability to biotransform the stimulatory signals. © 2016 Society of Chemical Industry

ACS Style

Angela Boari; Biancamaria Ciasca; Rocío Pineda-Martos; Veronica Mt Lattanzio; Koichi Yoneyama; Maurizio Vurro. Parasitic weed management by using strigolactone-degrading fungi. Pest Management Science 2016, 72, 2043 -2047.

AMA Style

Angela Boari, Biancamaria Ciasca, Rocío Pineda-Martos, Veronica Mt Lattanzio, Koichi Yoneyama, Maurizio Vurro. Parasitic weed management by using strigolactone-degrading fungi. Pest Management Science. 2016; 72 (11):2043-2047.

Chicago/Turabian Style

Angela Boari; Biancamaria Ciasca; Rocío Pineda-Martos; Veronica Mt Lattanzio; Koichi Yoneyama; Maurizio Vurro. 2016. "Parasitic weed management by using strigolactone-degrading fungi." Pest Management Science 72, no. 11: 2043-2047.

Journal article
Published: 01 February 2016 in TrAC Trends in Analytical Chemistry
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Recent advances in evaluation of analytical performances of semi-quantitative immunoassays through single-laboratory validation are presented. Emphasis is given to guidelines set in the Regulation 519/2014/EU that specifies validation criteria for mycotoxin screening methods to be used for official control purposes. A commercial lateral flow immunoassay for deoxynivalenol has been selected as case study and evaluated for its applicability to verify wheat compliance with EC maximum permitted level. The validation design provided information on the precision profile of the method, cut-off, false suspect and false negative rates of samples containing deoxynivalenol above the legal limit. Furthermore, the influence of the co-occurrence of major deoxynivalenol modified forms, i.e. 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, and deoxynivalenol-3-glucoside on test results, was evaluated by a factorial design. Finally, the applicability of the validated immunoassay was demonstrated by analysis of a set of 47 naturally contaminated wheat samples, and comparison with results obtained by a LC-MS/MS confirmatory method.JRC.F.5-Food and Feed Complianc

ACS Style

Veronica M.T. Lattanzio; Biancamaria Ciasca; Stephen Powers; Christoph von Holst. Validation of screening methods according to Regulation 519/2014/EU. Determination of deoxynivalenol in wheat by lateral flow immunoassay: A case study. TrAC Trends in Analytical Chemistry 2016, 76, 137 -144.

AMA Style

Veronica M.T. Lattanzio, Biancamaria Ciasca, Stephen Powers, Christoph von Holst. Validation of screening methods according to Regulation 519/2014/EU. Determination of deoxynivalenol in wheat by lateral flow immunoassay: A case study. TrAC Trends in Analytical Chemistry. 2016; 76 ():137-144.

Chicago/Turabian Style

Veronica M.T. Lattanzio; Biancamaria Ciasca; Stephen Powers; Christoph von Holst. 2016. "Validation of screening methods according to Regulation 519/2014/EU. Determination of deoxynivalenol in wheat by lateral flow immunoassay: A case study." TrAC Trends in Analytical Chemistry 76, no. : 137-144.

Original articles
Published: 01 June 2015 in Food Additives & Contaminants: Part A
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This paper reports a new method for the determination of T-2 and HT-2 toxins and their glucosylated derivatives in cereals, and some survey data aimed at obtaining more comprehensive information on the co-occurrence of T-2 and HT-2 toxins and their glucosylated derivatives in naturally contaminated cereal samples. For these purposes, barley samples originating from a Northern Italian area were analysed by LC-HRMS for the presence of T-2, HT-2 and relevant glucosyl derivatives. Quantitative analysis of T-2 and HT-2 glucosides was performed for the first time using a recently made available standard of T-2 glucoside. The glucosyl derivative of HT-2 was detected at levels up to 163 µg kg–1 in 17 of the 18 analysed unprocessed barley grains, whereas the monoglucosyl derivative of T-2 toxin was detected in only a few samples and at low µg kg–1 levels. The ratio between glucosylated toxins (sum of T-2 and HT-2 glucosides) and native toxins (sum of T-2 and HT-2) ranged from 2% to 283%. Moreover, taking advantage of the possibility of retrospective analysis of full-scan HRMS chromatograms, samples were also screened for the presence of other type-A trichothecenes, namely neosolaniol, diacetoxyscirpenol and their monoglucosyl derivatives, which were detected at trace levels. A subset of nine different samples was subjected to micro-maltation in order to carry out a preliminary investigation on the fate of T-2, HT-2 and relevant glucosides along the malting process. Mycotoxin reduction from cleaned barley to malt was observed at rates ranging from 4% to 87%.

ACS Style

Veronica M.T. Lattanzio; Biancamaria Ciasca; Valeria Terzi; Roberta Ghizzoni; Susan McCormick; Michelangelo Pascale. Study of the natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high-resolution Orbitrap mass spectrometry. Food Additives & Contaminants: Part A 2015, 32, 1647 -1655.

AMA Style

Veronica M.T. Lattanzio, Biancamaria Ciasca, Valeria Terzi, Roberta Ghizzoni, Susan McCormick, Michelangelo Pascale. Study of the natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high-resolution Orbitrap mass spectrometry. Food Additives & Contaminants: Part A. 2015; 32 (10):1647-1655.

Chicago/Turabian Style

Veronica M.T. Lattanzio; Biancamaria Ciasca; Valeria Terzi; Roberta Ghizzoni; Susan McCormick; Michelangelo Pascale. 2015. "Study of the natural occurrence of T-2 and HT-2 toxins and their glucosyl derivatives from field barley to malt by high-resolution Orbitrap mass spectrometry." Food Additives & Contaminants: Part A 32, no. 10: 1647-1655.

Journal article
Published: 01 August 2014 in Journal of Chromatography A
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An improved method for the quantitative determination of aflatoxins (B1, B2, G1, G2), ochratoxin A, fumonisins (B1, B2), zearalenone, deoxynivalenol, nivalenol, T-2 and HT-2 toxins in cereals and derived products, at levels comparable with EU maximum permitted levels, was developed. The effective co-extraction of the mycotoxins under investigation was achieved in 4min by a double extraction approach, using water followed by methanol. Clean up of the extract was performed by a new multi-toxin immunoaffinity column. Analytical performance characteristics were evaluated through single laboratory validation. Raw wheat and maize, corn flakes and maize snacks were chosen as representative matrices for method validation. The validation assay was carried out at 50, 100 and 150% of EU maximum permitted levels for each mycotoxin. Statistical analysis of the results (ANOVA) provided the within laboratory reproducibility and the error contributions from repeatability, between day effects, and influences from different matrix composition. Recoveries generally higher than 70% were obtained for all tested mycotoxins with relative standard deviation (within laboratory reproducibility) lesser than 37%. Limits of quantification (calculated as the lowest amount of each analyte which could be determined with a precision of 10%) ranged from 1μg/kg to 30μg/kg. The trueness of generated data was assessed by analysis of reference materials. The proposed method was proven to be suitable to assess, with a single analysis, compliance of the selected cereal based foods with the EU maximum permitted or recommended levels for all regulated mycotoxins.

ACS Style

Veronica Maria Teresa Lattanzio; Biancamaria Ciasca; Stephen Powers; Angelo Visconti. Improved method for the simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in cereals and derived products by liquid chromatography–tandem mass spectrometry after multi-toxin immunoaffinity clean up. Journal of Chromatography A 2014, 1354, 139 -143.

AMA Style

Veronica Maria Teresa Lattanzio, Biancamaria Ciasca, Stephen Powers, Angelo Visconti. Improved method for the simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in cereals and derived products by liquid chromatography–tandem mass spectrometry after multi-toxin immunoaffinity clean up. Journal of Chromatography A. 2014; 1354 ():139-143.

Chicago/Turabian Style

Veronica Maria Teresa Lattanzio; Biancamaria Ciasca; Stephen Powers; Angelo Visconti. 2014. "Improved method for the simultaneous determination of aflatoxins, ochratoxin A and Fusarium toxins in cereals and derived products by liquid chromatography–tandem mass spectrometry after multi-toxin immunoaffinity clean up." Journal of Chromatography A 1354, no. : 139-143.

Journal article
Published: 04 December 2013 in Journal of Mass Spectrometry
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Fusarium langsethiae, formally described as a new species over a decade ago, has been identified as the main producer of HT‐2 (HT2) and T‐2 (T2) toxins in Europe in small cereal grains. Mycotoxin contamination caused by this Fusarium species can represent a food safety hazard that deserves further attention. In the present work, the mycotoxin profile in wheat cultures of F. langsethiae is presented with particular reference to the production of major type‐A trichothecenes and their glucosyl derivatives. F. langsethiae isolates, representative of the major Italian wheat cultivation areas, were tested for the production of T2, HT2, diacetoxyscirpenol (DAS) and neosolaniol (NEO), and relevant glucosyl derivatives. Liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) was used for the identification and chemical characterization of these metabolites. F. langsethiae isolates under investigation resulted to be potent producers of T2, HT2 and NEO. Furthermore, a well‐defined set of isolates, all originating from Central Italy, produced also DAS. All isolates were found to be able to produce HT2 glucosyl derivatives, whereas only traces of T2 glucoside were detected in one sample. Furthermore, two mono‐glucosyl derivatives of NEO and one mono‐glucoside derivative of DAS were identified and characterized. The screening for the presence/absence of glucosylated trichothecenes in analyzed fungal extracts revealed a general co‐occurrence of these derivatives with the parent toxin at levels that could be roughly estimated to account up to 37% of the relevant unconjugated toxin. This is the first report of the production of glucosylated trichothecenes by F. langsethiae cultured on small grains. Copyright © 2013 John Wiley & Sons, Ltd.

ACS Style

Veronica M. T. Lattanzio; Biancamaria Ciasca; Edith Miriam Haidukowski; Alessandro Infantino; Angelo Visconti; Michelangelo Pascale. Mycotoxin profile ofFusarium langsethiaeisolated from wheat in Italy: production of type-A trichothecenes and relevant glucosyl derivatives. Journal of Mass Spectrometry 2013, 48, 1291 -1298.

AMA Style

Veronica M. T. Lattanzio, Biancamaria Ciasca, Edith Miriam Haidukowski, Alessandro Infantino, Angelo Visconti, Michelangelo Pascale. Mycotoxin profile ofFusarium langsethiaeisolated from wheat in Italy: production of type-A trichothecenes and relevant glucosyl derivatives. Journal of Mass Spectrometry. 2013; 48 (12):1291-1298.

Chicago/Turabian Style

Veronica M. T. Lattanzio; Biancamaria Ciasca; Edith Miriam Haidukowski; Alessandro Infantino; Angelo Visconti; Michelangelo Pascale. 2013. "Mycotoxin profile ofFusarium langsethiaeisolated from wheat in Italy: production of type-A trichothecenes and relevant glucosyl derivatives." Journal of Mass Spectrometry 48, no. 12: 1291-1298.