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Dr. Demir Ozdemir
Akdeniz University, Faculty of Agriculture, Department of Agricultural Biotechnology

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0 Poultry
0 Poultry Genetics
0 animal biotechnology
0 poultry breed conservation
0 population Genetics

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Journal article
Published: 01 April 2021 in BioTechniques
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The aim of this study was to assess the DNA preservation capability of cellulose paper towel and blotting paper as low-cost alternatives to commercial DNA preservation products. Chicken blood was applied as DNA source to each paper towel, blotting paper, FTA® cards and DNA/RNA Shield™. All samples were stored at room temperature for 130 days. DNA extraction from dried blood spots was performed after various time periods using Tris–EDTA and NaOH protocols. PCR activity and the mean amount of DNA isolated from paper towels were reliable. The results of this study demonstrated that cellulose-based blotting paper and especially paper towel had considerable DNA binding and preservation capacity for at least 130 days at room temperature without DNA degradation.

ACS Style

Demir Özdemir. A practical, low-cost, short-term storage method for genomic DNA. BioTechniques 2021, 70, 194 -201.

AMA Style

Demir Özdemir. A practical, low-cost, short-term storage method for genomic DNA. BioTechniques. 2021; 70 (4):194-201.

Chicago/Turabian Style

Demir Özdemir. 2021. "A practical, low-cost, short-term storage method for genomic DNA." BioTechniques 70, no. 4: 194-201.

Journal article
Published: 09 December 2020 in Animals
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The aim of this study was to collect, for the first time, comprehensive information about the backyard poultry sector in Turkey. This included a profile of the poultry owners, flock characteristics, husbandry, housing conditions, the owners’ biosecurity measures and the challenges of backyard poultry farming in residential areas of Turkey. An online semi-structured survey was fully completed by 1094 respondents. The majority of respondents (91%) owned fewer than 50 birds and reported raising other poultry species besides chickens (64%). Most of the participants indicated that they kept poultry as food for family use (83%) and had been involved in chicken-raising activities for less than 10 years (86%). Free-range coops were the type of housing most preferred by the respondents (86%). However, there was a lack of awareness about poultry diseases and poultry health care conditions. Respondents that confirmed wild bird and rodent access to their feeders and drinkers reported high rates of internal-external parasites, Escherichia coli infections and chronic respiratory diseases (p < 0.001). Lack of regional regulations (84%), animal theft (80%), lack of information on poultry management (79%), minimizing predation (75%), and the need for vaccination and veterinary services (73%) were the most cited challenges. The results highlighted the need for improved biosecurity measures and for a regulatory framework that takes into account the risks to commercial poultry flocks and public health.

ACS Style

Demir Özdemir. The Structural Characteristics, Management, and Challenges of Backyard Poultry Farming in Residential Areas of Turkey. Animals 2020, 10, 2336 .

AMA Style

Demir Özdemir. The Structural Characteristics, Management, and Challenges of Backyard Poultry Farming in Residential Areas of Turkey. Animals. 2020; 10 (12):2336.

Chicago/Turabian Style

Demir Özdemir. 2020. "The Structural Characteristics, Management, and Challenges of Backyard Poultry Farming in Residential Areas of Turkey." Animals 10, no. 12: 2336.

Journal article
Published: 01 November 2017 in Poultry Science
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ACS Style

E. Viale; E. Zanetti; Demir Ozdemir; C. Broccanello; Alessandra Dalmasso; M. De Marchi; M. Cassandro. Development and validation of a novel SNP panel for the genetic characterization of Italian chicken breeds by next-generation sequencing discovery and array genotyping. Poultry Science 2017, 96, 3858 -3866.

AMA Style

E. Viale, E. Zanetti, Demir Ozdemir, C. Broccanello, Alessandra Dalmasso, M. De Marchi, M. Cassandro. Development and validation of a novel SNP panel for the genetic characterization of Italian chicken breeds by next-generation sequencing discovery and array genotyping. Poultry Science. 2017; 96 (11):3858-3866.

Chicago/Turabian Style

E. Viale; E. Zanetti; Demir Ozdemir; C. Broccanello; Alessandra Dalmasso; M. De Marchi; M. Cassandro. 2017. "Development and validation of a novel SNP panel for the genetic characterization of Italian chicken breeds by next-generation sequencing discovery and array genotyping." Poultry Science 96, no. 11: 3858-3866.

Animal genetics and breeding
Published: 05 October 2017 in Italian Journal of Animal Science
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The aim of this study was to characterise the genetic diversity, genetic relationship and population structure within local Turkish Denizli chicken subpopulations in rural areas using 19 microsatellite markers. To assess the uniqueness and relations of the Denizli subpopulations we used six local Italian chicken breeds’ samples, which were genotyped in the same laboratory with the same molecular markers. In total, 105 alleles were found across 19 microsatellite loci with a mean number of 5.53 alleles per locus. Considering all subpopulations and loci, genetic differentiation based on global FST was 0.030 (p < .01). Global FIS values (0.200) indicated that non-random mating occurred in all subpopulations of Denizli fowl and all subpopulations deviated significantly (p < .01) from HWE. Over all subpopulations, the mean observed heterozygosity was 0.473, ranging from 0.399 to 0.562. Genetic differentiations between pairs of subpopulations based on the proportion of shared alleles ranged 0.140–0.297. The neighbour-net tree, based on marker estimated kinship distances, separated Denizli subpopulations and Italian breeds into two main clusters. The most likely number of different populations was estimated using the clustering procedure implemented in STRUCTURE. Structure analysis showed a clear separation of the Denizli fowl subpopulations from the Italian populations. A second step sub-clustering allowed discriminating among the six subpopulations of Denizli breed. The results of this study can be used as baseline genetic information to place breeders’ flocks in conservation programmes, controlling inbreeding and safeguarding the genetic variability of the populations.

ACS Style

Demir Özdemir; Martino Cassandro. Assessment of the population structure and genetic diversity of Denizli chicken subpopulations using SSR markers. Italian Journal of Animal Science 2017, 17, 312 -320.

AMA Style

Demir Özdemir, Martino Cassandro. Assessment of the population structure and genetic diversity of Denizli chicken subpopulations using SSR markers. Italian Journal of Animal Science. 2017; 17 (2):312-320.

Chicago/Turabian Style

Demir Özdemir; Martino Cassandro. 2017. "Assessment of the population structure and genetic diversity of Denizli chicken subpopulations using SSR markers." Italian Journal of Animal Science 17, no. 2: 312-320.

Non ruminants nutrition and feeding
Published: 02 August 2017 in Italian Journal of Animal Science
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The current study aimed to compare the effects of the dietary supplementation of oleuropein (O) alone or with organic selenium (OSe) in combination with the dietary supplementation of α-tocopherol acetate alone (TA) or with OSe in combination on tonic immobility (TI) duration and fluctuating asymmetry in Japanese quails exposed to heat stress (HS) (34 °C). A total of 800 two-week old quails were kept in wire cages in a temperature-controlled room at either 22 °C (thermo-neutral, TN) or 34 °C (HS) for 8 h/d and fed on a basal diet (NC) or the diets supplemented with TA (TA200) or O (O200) at 200 mg/kg alone or with OSe (TA200 + OSe and O200 + OSe) to the NC diet. HS increased TI (p < .001) and the lengths of the beak, outer toe, eye and nostril (p < .05) of quails. On the other hand, feeding diets containing TA200 alone or with OSe and O200 with OSe significantly shorted the TI duration and decreased the nostril length of quails exposed to HS compared to those of quails fed the NC and O200 diets (p < .05). It can be suggested that the TI and the asymmetry value are a sensitive indicator of thermal stress’ effect in quails. Moreover, the present study showed that especially the O200 + OSe diet may similarly alleviate the negative effects of HS on TI and mean bilateral asymmetry value of the nostril length in quail compared to the TA200 and TA200 + OSe diets.

ACS Style

Senay Sarica; Demir Ozdemir. The effects of dietary oleuropein and organic selenium supplementation in heat-stressed quails on tonic immobility duration and fluctuating asymmetry. Italian Journal of Animal Science 2017, 17, 145 -152.

AMA Style

Senay Sarica, Demir Ozdemir. The effects of dietary oleuropein and organic selenium supplementation in heat-stressed quails on tonic immobility duration and fluctuating asymmetry. Italian Journal of Animal Science. 2017; 17 (1):145-152.

Chicago/Turabian Style

Senay Sarica; Demir Ozdemir. 2017. "The effects of dietary oleuropein and organic selenium supplementation in heat-stressed quails on tonic immobility duration and fluctuating asymmetry." Italian Journal of Animal Science 17, no. 1: 145-152.

Journal article
Published: 01 January 2015 in Italian Journal of Animal Science
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The study aimed to investigate whether dietary supplementation of oleuropein (O) alone or with organic selenium (OSe) will show the similar effects as the dietary supplementation of α-tocopherol acetate (TA) on performance, blood Heterophil/Lymphocyte ratio (H/L ratio) and brain Hsp70 gene in Japanese quails reared under heat stress (34°C). A total of 800 Japanese quails were kept in wire cages in a temperature-controlled room at either 22°C (thermoneutral, TN) or 34°C (heat stress, HS) for 8 h/days and fed 5 different dietary treatments. Quails were fed on a basal diet (NC) or the diets supplemented with TA (TA200) or O (O200) at 200 mg/kg alone or with OSe (TA200+OSe and O200+OSe) to NC. HS decreased the final body weights (BWs) (P<0.05), the body weight gains (BWGs) (P<0.01) and feed intake (FI) (P<0.05) and also deteriorated feed conversion ratio (FCR) (P<0.05) of quails from 21 to 35 d of age. Moreover, feeding with the TA200, O200, TA200+OSe and O200+OSe diets increased final BWs and BWGs and FI of quails from 21 to 35 d of age (P<0.05). While HS increased blood H/L ratio (P<0.05) and brain Hsp70 gene expression (P<0.001). All dietary treatments except the NC diet on blood H/L ratio and O200+OSe on brain Hsp70 gene expression had positive effects. Consequently, the present study showed that supplementation of O alone or with OSe eliminated the deleterious effects of heat stress on growth performance, blood H/L ratio and brain Hsp70 gene expression of Japanese quails.

ACS Style

Şenay Sarıca; Demir Ozdemir; Hakan Öztürk. The Effects of Dietary Oleuropein and Organic Selenium Supplementation on Performance and Heat Shock Protein 70 Response of Brain in Heat-Stressed Quail. Italian Journal of Animal Science 2015, 14, 1 .

AMA Style

Şenay Sarıca, Demir Ozdemir, Hakan Öztürk. The Effects of Dietary Oleuropein and Organic Selenium Supplementation on Performance and Heat Shock Protein 70 Response of Brain in Heat-Stressed Quail. Italian Journal of Animal Science. 2015; 14 (2):1.

Chicago/Turabian Style

Şenay Sarıca; Demir Ozdemir; Hakan Öztürk. 2015. "The Effects of Dietary Oleuropein and Organic Selenium Supplementation on Performance and Heat Shock Protein 70 Response of Brain in Heat-Stressed Quail." Italian Journal of Animal Science 14, no. 2: 1.

Journal article
Published: 01 January 2014 in Italian Journal of Animal Science
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ACS Style

Şenay Sarıca; Menderes Suiçmez; Muzaffer Çördük; Demir Ozdemir; Emine Berberoglu; Senay Sarica. Effects of Oregano Essential Oil Supplementation to Diets of Broiler Chicks with Delayed Feeding After Hatching. Morphological Development of Small Intestine Segments. Italian Journal of Animal Science 2014, 13, 3172 .

AMA Style

Şenay Sarıca, Menderes Suiçmez, Muzaffer Çördük, Demir Ozdemir, Emine Berberoglu, Senay Sarica. Effects of Oregano Essential Oil Supplementation to Diets of Broiler Chicks with Delayed Feeding After Hatching. Morphological Development of Small Intestine Segments. Italian Journal of Animal Science. 2014; 13 (2):3172.

Chicago/Turabian Style

Şenay Sarıca; Menderes Suiçmez; Muzaffer Çördük; Demir Ozdemir; Emine Berberoglu; Senay Sarica. 2014. "Effects of Oregano Essential Oil Supplementation to Diets of Broiler Chicks with Delayed Feeding After Hatching. Morphological Development of Small Intestine Segments." Italian Journal of Animal Science 13, no. 2: 3172.

Journal article
Published: 01 July 2013 in Journal of Applied Poultry Research
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Temperature acclimation, either during the incubation or the early growing period, has been suggested as an effective method to improve thermotolerance of broilers to cooler rearing temperatures. We compared the response of broilers from younger (Y) and older (O) breeder flocks to cooler rearing temperatures after temperature acclimation during incubation and early posthatch. Eggs were exposed to either a control incubation temperature (37.6°C) or a cooler temperature (36.6°C; ITcool) for 6 h (from1000 to 1600 h) daily from d 10 to 18 of incubation. From d 22 to 42, chicks were reared at a control temperature (RTcont), a cooler temperature (17 ± 2°C; RTcool), or at a cooler temperature after acclimation of chicks to cooler temperatures by exposing them to 17 ± 2°C for 6 h on d 5 posthatch. The ITcool conditions reduced mortality and mortality due to ascites of O broilers reared under cooler temperatures from d 22 to 42. Early age acclimation to cooler temperatures did not support thermotolerance of broilers. Regardless of IT, the RTcool broilers from Y gained more weight but had poorer feed conversion than RTcont. Repeated cooler temperatures applied during incubation and early postnatal stage disturbed homeostasis and energy balance of broilers from O breeders.

ACS Style

M. Akşit; S. Yalçın; P. B. Siegel; Ç. Yenisey; Demir Ozdemir; S. Özkan. Broilers respond to cooler ambient temperatures after temperature acclimation during incubation and early postnatal age. Journal of Applied Poultry Research 2013, 22, 298 -307.

AMA Style

M. Akşit, S. Yalçın, P. B. Siegel, Ç. Yenisey, Demir Ozdemir, S. Özkan. Broilers respond to cooler ambient temperatures after temperature acclimation during incubation and early postnatal age. Journal of Applied Poultry Research. 2013; 22 (2):298-307.

Chicago/Turabian Style

M. Akşit; S. Yalçın; P. B. Siegel; Ç. Yenisey; Demir Ozdemir; S. Özkan. 2013. "Broilers respond to cooler ambient temperatures after temperature acclimation during incubation and early postnatal age." Journal of Applied Poultry Research 22, no. 2: 298-307.

Journal article
Published: 01 January 2013 in Italian Journal of Animal Science
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Increased global use of highly productive breeds of farm animals has been associated with loss of genetic diversity in most species, but especially in local poultry species. In Italy, especially in the Veneto region since 2000, various governmental, non-governmental and private organizations have tried to preserve the genetic diversity of poultry resources. This successful conservation effort has included various activities: improving knowledge of biological functions, conservation of typical morphological characteristics, development of selection strategies, control of inbreeding and, finally, valorization strategies to diffuse the breed in local productive systems. These activities provide a good example for developing countries, such as Turkey. With the aim of contributing to conservation activities relating to the poultry resources of Turkey, this study described the current status of local chicken breeds in Turkey and Italy, and also makes some recommendations for developing countries such as Turkey.

ACS Style

Demir Ozdemir; Eren Durmuş Özdemir; Massimo De Marchi; Martino Cassandro. Conservation of Local Turkish and Italian Chicken Breeds: A Case Study. Italian Journal of Animal Science 2013, 12, 1 .

AMA Style

Demir Ozdemir, Eren Durmuş Özdemir, Massimo De Marchi, Martino Cassandro. Conservation of Local Turkish and Italian Chicken Breeds: A Case Study. Italian Journal of Animal Science. 2013; 12 (2):1.

Chicago/Turabian Style

Demir Ozdemir; Eren Durmuş Özdemir; Massimo De Marchi; Martino Cassandro. 2013. "Conservation of Local Turkish and Italian Chicken Breeds: A Case Study." Italian Journal of Animal Science 12, no. 2: 1.

Journal article
Published: 01 June 2010 in British Poultry Science
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1. The objective was to evaluate the effects of brooding temperature on intestinal development, oxidative organ damage, and performance of chicks acclimated to high temperature during incubation. The effects of acclimation and brooding temperatures on slaughter weights of broilers under heat stress were also investigated. 2. Eggs were incubated at either 37⋅8°C (INCCont) or heat-acclimated at 39⋅5°C for 6 h daily from d 10 to d 18 of incubation (INCH). Brooding temperatures at floor level were set at 32, 33⋅5 and 35°C (Bt32, Bt33⋅5, Bt35, respectively) for the first 5 d. The temperature was reduced gradually to 30°C from d 6 to d 10. From 21 to 42 d, broilers from INCCont Bt32 and INCH Bt32 and Bt35 were divided into two groups; half from each group was exposed to daily cyclic higher ambient temperatures, while the other half was reared at control temperature. 3. INCH chicks had lower jejunum, but greater liver and residual yolk sac weights than INCCont chicks on the day of hatching. Although INCH chicks from Bt33⋅5 and Bt35 had lighter body weights than Bt32 on d 5, no significant differences were observed in the body weight of broilers among treatments at 10 and 21 d. 4. Similar jejunum protein, alkaline phosphatase, maltase, glutathione, and malondialdehyde contents of chicks from INCCont and INCH suggested that heat acclimation during incubation has no effect on jejunum enzyme activity or oxidative status of chicks. 5. Taking into account INCH Bt35 chicks having lower T3 levels on d 5, lower heterophil/lymphocyte (H/L) ratios and similar weights at 42 d under heat stress compared with control broilers, the results suggested that although higher brooding temperatures had no effect on body weights of INCH chicks during the brooding period, those broilers may able to cope better with heat stress.

ACS Style

M. Akşit; S. Yalcin; C. Yenisey; Demir Ozdemir. Brooding temperatures for chicks acclimated to heat during incubation: effects on post-hatch intestinal development and body weight under heat stress. British Poultry Science 2010, 51, 444 -452.

AMA Style

M. Akşit, S. Yalcin, C. Yenisey, Demir Ozdemir. Brooding temperatures for chicks acclimated to heat during incubation: effects on post-hatch intestinal development and body weight under heat stress. British Poultry Science. 2010; 51 (3):444-452.

Chicago/Turabian Style

M. Akşit; S. Yalcin; C. Yenisey; Demir Ozdemir. 2010. "Brooding temperatures for chicks acclimated to heat during incubation: effects on post-hatch intestinal development and body weight under heat stress." British Poultry Science 51, no. 3: 444-452.

Journal article
Published: 01 November 2006 in Poultry Science
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Two trials were conducted to study the effects of heat stress during rearing (trial 1) and crating (trial 2) on broiler stress parameters and fear, breast meat quality, and nutrient composition. The relationships between stress parameters and meat quality traits were also determined. Trial 1 consisted of 3 temperature treatments from 3 to 7 wk: control (temperature was 22°C); diurnal cyclic temperature (temperature was 28°C from 1000 to 1700 h and 22°C from 1700 to 1000 h); and constant high temperature (34°C; temperature was 34°C). In trial 2, broilers from the control and 34°C groups in trial 1 were used. Broilers in each group were placed in transport cages. The 9 cages from the control group were divided into 3 groups and placed into 3 rooms at 15, 22, or 34°C for 2 h. The 3 cages from the 34°C group were also held in the room at 34°C (34–34°C). Diurnal cyclic temperature had no effect on BW up to 5 wk of age. The effect of 34°C constant temperature on BW of broilers increased with age. Plasma levels of glucose and albumin increased by 34°C, but no dramatic change in levels occurred when those broilers were crated at 34°C. The heterophil:lymphocyte (H:L) was higher for the 34–34°C broilers and the control broilers in the 34°C room than those from the 22 and 15°C room. Breast muscle glycogen level decreased in broilers reared under diurnal cyclic or high temperatures. A lower pH and higher lightness (L*) and redness values and redness:yellowness were found in meat for broilers from both 34°C and 34–34°C groups. Higher H:L was associated with breast muscle pH according to first-order polynomial regression. The H:L had a significant effect on L* values, which were described by a second-order polynomial regression. Blood glucose level was positively correlated with L* and redness values. Duration of tonic immobility was neither influenced by rearing and crating temperatures nor associated with meat quality parameters.

ACS Style

M. Akşit; S. Yalçin; S. Özkan; K. Metin; D. Özdemir. Effects of Temperature During Rearing and Crating on Stress Parameters and Meat Quality of Broilers. Poultry Science 2006, 85, 1867 -1874.

AMA Style

M. Akşit, S. Yalçin, S. Özkan, K. Metin, D. Özdemir. Effects of Temperature During Rearing and Crating on Stress Parameters and Meat Quality of Broilers. Poultry Science. 2006; 85 (11):1867-1874.

Chicago/Turabian Style

M. Akşit; S. Yalçin; S. Özkan; K. Metin; D. Özdemir. 2006. "Effects of Temperature During Rearing and Crating on Stress Parameters and Meat Quality of Broilers." Poultry Science 85, no. 11: 1867-1874.