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This study analyzes 174 tank milk samples taken from 89 commercial farms located all along the Cantabrian Coast (Green Spain). Sampling was performed in two periods: autumn 2016 and spring 2017. A survey was carried out for every day of sampling to record the average lactating dairy cow production and its diet composition. For each sample, the fatty acid (FA) profile (49 FA plus its main relationships) and nine fat-soluble antioxidant (FSA) profiles (retinol (vitamin A), α- and Υ-tocopherol (vitamin E), all-trans-β-carotene, 9-cis-β-carotene, 13-cis-β-carotene, lutein, zeaxanthin, and β-cryptoxanthin) were determined. The milk production varied between 7.3 and 45.9 liters per cow per day, highlighting the diversity found among production systems. The milk fat content ranged from 2.64% to 4.38% and the protein content from 2.87% to 3.56%. Regarding the fatty acids profile, the percentage of saturated fat varied between 59.95% and 75.99%. The linolenic acid content fluctuated between 0.21 and 1.31 and rumenic acid ranged from 0.20 to 2.47 (g 100 g−1 total FA). The most important correlations between diet and milk FA were always related to the content of fresh grass and total forage (which is defined by both fresh and conserved forage derived from fresh grass (GCF)) in the diet. The content of vaccenic acid, linolenic acid, total omega-3, rumenic acid, and total CLA isomers showed the highest correlation with the proportion of fresh grass in the diet. The antioxidant contents were also highly variable, although correlations with dietary components were lower. The highest correlations were between total forage content (fresh grass (FG) plus GCF) and lutein, all-trans-β-carotene, and 13-cis-β-carotene. Diets without fresh grass had lower omega-3 content, CLA, vaccenic acid, lutein, all-trans-β-carotene, and 13-cis-β-carotene.
Ana Villar; Ibán Vázquez-González; Fernando Vicente; Gregorio Salcedo; Laura González; Adrián Botana; Luís Royo; Paola Eguinoa; Juan Busqué. Study of the Variability in Fatty Acids and Carotenoid Profiles: Laying the Ground for Tank Milk Authentication. Sustainability 2021, 13, 4506 .
AMA StyleAna Villar, Ibán Vázquez-González, Fernando Vicente, Gregorio Salcedo, Laura González, Adrián Botana, Luís Royo, Paola Eguinoa, Juan Busqué. Study of the Variability in Fatty Acids and Carotenoid Profiles: Laying the Ground for Tank Milk Authentication. Sustainability. 2021; 13 (8):4506.
Chicago/Turabian StyleAna Villar; Ibán Vázquez-González; Fernando Vicente; Gregorio Salcedo; Laura González; Adrián Botana; Luís Royo; Paola Eguinoa; Juan Busqué. 2021. "Study of the Variability in Fatty Acids and Carotenoid Profiles: Laying the Ground for Tank Milk Authentication." Sustainability 13, no. 8: 4506.
The optimization of milk production includes a rational use of forages, respect for the environment and offers the best quality to consumers. Milk production based on grass and forages produces healthier milk and it is widely spread throughout the Atlantic arc to maximize milk yield per hectare. However, the mode of offering the grass can have a major influence on milk composition. The aim of this study was to evaluate the effect of grass supply mode (grazing, zero-grazing or ensiling) on dairy cows’ performance, with particular reference to fatty acids and fat-soluble antioxidants concentration. A three by three Latin square experiment was performed with 18 dairy cows. Experimental treatments consisted of exclusive feeding with grass silage and zero-grazing, both offered ad libitum indoors, or grazing for 24 h. The results showed that grazing cows had a higher dry matter intake and greater milk yield than cows feeding on grass silage and zero-grazing, as well as higher concentrations of protein, lactose, nonfat-solids and urea in milk than housed cows. Milk fat from grazing cows had a higher proportion of unsaturated fatty acids than from cows feeding on grass silage and zero-grazing, with significant differences in the proportion of vaccenic and rumenic acids. The 18:1 trans-11 to 18:1 trans-10 ratio is proposed as biomarker to identify the milk produced from the management system of grazing cattle. Milk from grazing cows had a greater proportion of lutein than cows eating grass silage, with the zero-grazing system having intermediate values. In conclusion, the mode of grass supply affects fatty acid and antioxidant profiles of milk.
Senén De La Torre-Santos; Luis Royo; Adela Martínez-Fernández; Cristina Chocarro; Fernando Vicente. The Mode of Grass Supply to Dairy Cows Impacts on Fatty Acid and Antioxidant Profile of Milk. Foods 2020, 9, 1256 .
AMA StyleSenén De La Torre-Santos, Luis Royo, Adela Martínez-Fernández, Cristina Chocarro, Fernando Vicente. The Mode of Grass Supply to Dairy Cows Impacts on Fatty Acid and Antioxidant Profile of Milk. Foods. 2020; 9 (9):1256.
Chicago/Turabian StyleSenén De La Torre-Santos; Luis Royo; Adela Martínez-Fernández; Cristina Chocarro; Fernando Vicente. 2020. "The Mode of Grass Supply to Dairy Cows Impacts on Fatty Acid and Antioxidant Profile of Milk." Foods 9, no. 9: 1256.
This work summarizes the mortality cases of twenty-five free-ranging Eurasian wild brown bears (Ursus arctos arctos) from the Cantabrian mountain range submitted for necropsy in Asturias and Castilla y León (northwestern Spain) from 1998 to 2018. Mortality cases were classified both caused by (i) "non-human intervention" or "human intervention” causes and based on (ii) "non-infectious" or "infectious" etiology. In four cases (16%) it was not possible to determine the cause of death due to the inadequate preservation of collected specimens or insufficient tissue availability. Based on "non-human intervention" or "human intervention” causes, fourteen of the 21 (66.7%) brown bears died as a consequence of "non-human intervention" due to traumatic lesions (fights, unknown traumas or infanticide), infectious canine hepatitis, neoplasia or mushroom poisoning. In contrast, seven (33.3%) brown bears died by “human intervention” due to illegal hunting (shooting or snare), handling (during transit in an attempt to reintroduce a bear back into the wild) or strychnine poisoning. Based on "non-infectious" or "infectious" etiology, twelve of the 21 (57.1%) brown bears died due to "non-infectious” causes, namely traumatic lesions such as shooting, snare, fighting or infanticide, handling, strychnine poisoning, mushroom poisoning or neoplasia. The remaining nine (42.9%) animals died due to “infectious” diseases which included gangrenous myositis, infectious canine hepatitis or septicemia. In six of those cases traumatic lesions caused by non-human or human activities were complicated with bacterial infection (clostridiosis and septicemia) which finally caused the death of those animals. Additionally, exertional myopathy was observed in the handled animal and in one bear found in a snare. In a free-ranging population of Eurasian brown bear from the Cantabrian mountain range, main causes of death are attributed to non-human related traumatic lesions and infectious diseases (primary developed such as infectious canine hepatitis or secondary developed such as clostridiosis or septicemia) which is in contrast to previously reported data for other bear populations. These data are valuable and may help in the conservation and management of this recovering population.
Ana Balseiro; Luis Royo; Elena Gayo; Ramón Balsera; Olga Alarcia; Juan García Marín. Mortality Causes in Free-Ranging Eurasian Brown Bears (Ursus arctos arctos) in Spain 1998–2018. Animals 2020, 10, 1538 .
AMA StyleAna Balseiro, Luis Royo, Elena Gayo, Ramón Balsera, Olga Alarcia, Juan García Marín. Mortality Causes in Free-Ranging Eurasian Brown Bears (Ursus arctos arctos) in Spain 1998–2018. Animals. 2020; 10 (9):1538.
Chicago/Turabian StyleAna Balseiro; Luis Royo; Elena Gayo; Ramón Balsera; Olga Alarcia; Juan García Marín. 2020. "Mortality Causes in Free-Ranging Eurasian Brown Bears (Ursus arctos arctos) in Spain 1998–2018." Animals 10, no. 9: 1538.
Having rapid and reliable knowledge of the quality of dairy farm forage is crucial for technicians and producers. However, the necessary equipment to fulfil these requirements is oftentimes expensive and complex to use. This work presents a portable instrument system to analyze the nutritional values of dairy farm forage using near-infrared spectrometry (NIRS) techniques. Using the Internet of Things (IoT) tools, data are sent to the cloud for processing; following this, they are accessible to any device. To analyze the nutritional values of dairy farm forage a chemometric model and implemented the instrument to understand the relationship between the measured spectrum and the concentration of the substances of interest was developed. The quality of the proposed methodology was validated by comparing reference and NIR data by using the statistic Standard Error of Calibration.
Guillermo Rego; Francisco Ferrero; Marta Valledor; Juan Carlos Campo; Sergio Forcada; Luis J. Royo; Ana Soldado. A portable IoT NIR spectroscopic system to analyze the quality of dairy farm forage. Computers and Electronics in Agriculture 2020, 175, 105578 .
AMA StyleGuillermo Rego, Francisco Ferrero, Marta Valledor, Juan Carlos Campo, Sergio Forcada, Luis J. Royo, Ana Soldado. A portable IoT NIR spectroscopic system to analyze the quality of dairy farm forage. Computers and Electronics in Agriculture. 2020; 175 ():105578.
Chicago/Turabian StyleGuillermo Rego; Francisco Ferrero; Marta Valledor; Juan Carlos Campo; Sergio Forcada; Luis J. Royo; Ana Soldado. 2020. "A portable IoT NIR spectroscopic system to analyze the quality of dairy farm forage." Computers and Electronics in Agriculture 175, no. : 105578.
Gold nanoparticles of different sizes have been synthesized and surface-functionalized with selected RNA probes in order to develop a rapid, low-cost and sensitive method for detection of microRNA146a (miR146a). The strategy is based on the change of colour that can be observed visually after aggregation of the RNA modified-gold nanoparticles (AuNPs) in presence of miR146a. Experimental conditions have been carefully selected in order to obtain a good sensitivity that allows to perform visual detection of microRNA at the nM level, achieving a detection limit of 5 nM. Good repeatability and selectivity versus other sequences that only differ from miR146a in 3 bases was achieved. miR146a has been described as one of the main microRNA involved in the immune response of bovine mastitis, being expressed in tissue, blood and milk samples. The method was successfully applied to the detection of miR146a in raw cow milk samples. The present scheme constitutes a rapid and low-cost alternative to perform highly sensitive detection of microRNA without the need of instrumentation and amplification steps for the early detection of bovine mastitis in the agrofood industry. Graphical abstract Schematic representation of the assay based on aggregation of RNA-modified gold nanoparticles (blue) in presence of microRNA146a generating a dark blue spot onto a solid support, versus a pink spot observed in absence of miR146a due to dispersed gold nanoparticles (red).
Adrián Sánchez-Visedo; Borja Gallego; Luis José Royo; Ana Soldado; Marta Valledor; Francisco Javier Ferrero; Juan Carlos Campo; José Manuel Costa-Fernández; María Teresa Fernández-Argüelles. Visual detection of microRNA146a by using RNA-functionalized gold nanoparticles. Microchimica Acta 2020, 187, 192 -9.
AMA StyleAdrián Sánchez-Visedo, Borja Gallego, Luis José Royo, Ana Soldado, Marta Valledor, Francisco Javier Ferrero, Juan Carlos Campo, José Manuel Costa-Fernández, María Teresa Fernández-Argüelles. Visual detection of microRNA146a by using RNA-functionalized gold nanoparticles. Microchimica Acta. 2020; 187 (3):192-9.
Chicago/Turabian StyleAdrián Sánchez-Visedo; Borja Gallego; Luis José Royo; Ana Soldado; Marta Valledor; Francisco Javier Ferrero; Juan Carlos Campo; José Manuel Costa-Fernández; María Teresa Fernández-Argüelles. 2020. "Visual detection of microRNA146a by using RNA-functionalized gold nanoparticles." Microchimica Acta 187, no. 3: 192-9.
Ana Balseiro; Luis José Royo; Elena Gayo; Juan Francisco García Marín. Cholangiocarcinoma in a Free-Ranging Eurasian Brown Bear (Ursus arctos arctos) from Northern Spain. Journal of Wildlife Diseases 2020, 56, 251 .
AMA StyleAna Balseiro, Luis José Royo, Elena Gayo, Juan Francisco García Marín. Cholangiocarcinoma in a Free-Ranging Eurasian Brown Bear (Ursus arctos arctos) from Northern Spain. Journal of Wildlife Diseases. 2020; 56 (1):251.
Chicago/Turabian StyleAna Balseiro; Luis José Royo; Elena Gayo; Juan Francisco García Marín. 2020. "Cholangiocarcinoma in a Free-Ranging Eurasian Brown Bear (Ursus arctos arctos) from Northern Spain." Journal of Wildlife Diseases 56, no. 1: 251.
Lactobacillus genus includes both probiotic and representative strains of the human gut microbiota. Independent studies have reported on the anti-inflammatory properties of different Lactobacillus strains, although we are far from understanding the underlying molecular interplay. In this work we show that a daily administration of Lactobacillus acidophilus DSM20079T (DSM20079) to healthy piglets resulted in plasmatic increases of the anti-inflammatory IL10, whilst IL12 and the pro-inflammatory ratio IL12+TNFα/IL10 decreased. The extracellular protein fraction of DSM20079 was identified as the responsible for the crosstalk interaction that elicited these tolerogenic effects. This strain was able to activate innate immune pathways in dendritic cells and to decrease the production of pro-inflammatory cytokines in both CD4+/CD8+ T cell subsets in healthy donors and in Crohn’s Disease patients. The tolerogenic effect exerted by the extracellular proteins of this strain suggests their potential use as coadjutant for therapeutic applications targeting chronic inflammatory illnesses.
Claudio Hidalgo-Cantabrana; Marco Antonio Moro-García; Aitor Blanco-Míguez; Florentino Fdez-Riverola; Mamen Oliván; Luis J. Royo; Sabino Riestra; Abelardo Margolles; Anália Lourenço; Rebeca Alonso-Arias; Borja Sánchez. The extracellular proteins of Lactobacillus acidophilus DSM 20079T display anti-inflammatory effect in both in piglets, healthy human donors and Crohn’s Disease patients. Journal of Functional Foods 2019, 64, 103660 .
AMA StyleClaudio Hidalgo-Cantabrana, Marco Antonio Moro-García, Aitor Blanco-Míguez, Florentino Fdez-Riverola, Mamen Oliván, Luis J. Royo, Sabino Riestra, Abelardo Margolles, Anália Lourenço, Rebeca Alonso-Arias, Borja Sánchez. The extracellular proteins of Lactobacillus acidophilus DSM 20079T display anti-inflammatory effect in both in piglets, healthy human donors and Crohn’s Disease patients. Journal of Functional Foods. 2019; 64 ():103660.
Chicago/Turabian StyleClaudio Hidalgo-Cantabrana; Marco Antonio Moro-García; Aitor Blanco-Míguez; Florentino Fdez-Riverola; Mamen Oliván; Luis J. Royo; Sabino Riestra; Abelardo Margolles; Anália Lourenço; Rebeca Alonso-Arias; Borja Sánchez. 2019. "The extracellular proteins of Lactobacillus acidophilus DSM 20079T display anti-inflammatory effect in both in piglets, healthy human donors and Crohn’s Disease patients." Journal of Functional Foods 64, no. : 103660.
Ana Balseiro; Luis Royo; Elena Gayo; Juan Francisco García Marín. CHOLANGIOCARCINOMA IN A FREE-RANGING EURASIAN BROWN BEAR (URSUS ARCTOS ARCTOS) FROM NORTHERN SPAIN. 2019, 1 .
AMA StyleAna Balseiro, Luis Royo, Elena Gayo, Juan Francisco García Marín. CHOLANGIOCARCINOMA IN A FREE-RANGING EURASIAN BROWN BEAR (URSUS ARCTOS ARCTOS) FROM NORTHERN SPAIN. . 2019; ():1.
Chicago/Turabian StyleAna Balseiro; Luis Royo; Elena Gayo; Juan Francisco García Marín. 2019. "CHOLANGIOCARCINOMA IN A FREE-RANGING EURASIAN BROWN BEAR (URSUS ARCTOS ARCTOS) FROM NORTHERN SPAIN." , no. : 1.
Canine adenovirus type 1 (CAdV‐1) is responsible for infectious canine hepatitis. The disease has been described in captive American black bear (Ursus americanus) and European brown bear (Ursus arctos arctos), with just one recently reported case in a cub of a free‐ranging brown bear (Ursus arctos horribilis) from Alaska. The aim of this work is to summarize findings related to presence and associated mortality of CAdV‐1 in 21 free‐ranging Cantabrian brown bears (Ursus arctos arctos) submitted to necropsy in Asturias and Castilla y León (northwestern Spain) from 1998 to 2018. On the basis of the anatomopathological findings and laboratory results three free‐ranging brown bears died due to infectious canine hepatitis, which is to our knowledge the first description of death due to this disease in free‐ranging bears in Europe. Gross lesions consisted of petechial haemorrhages and congestion in different internal organs, haemorrhagic fluid in internal cavities, friable and yellowish liver and thickening of gall bladder. Microscopic lesions were observed mainly in liver, kidney and brain and consisted of multifocal necrosis of cells with presence of basophilic intranuclear inclusions. Immunohistochemical (IHC) and real‐time polymerase chain reaction (qPCR) techniques were used to assess the presence of CAdV‐1 in paraffin‐embedded liver samples. Viral antigens were detected by IHC labelling within hepatocytes and Küppfer cells in the three animals. The presence of viral DNA was confirmed by qPCR in one of them. In order to evaluate the circulation of CAdV‐1 in brown bears, a retrospective study was performed using both IHC and qPCR techniques in 11 and 12 additional brown bears, respectively. An extra brown bear was found positive by IHC. This study shows that CAdV‐1 surveillance of brown bears and sympatric carnivores should be considered as major concern for the monitoring the population evolution throughout time in this endangered species.
Juan F. García Marín; Luis J. Royo; Alvaro Oleaga; Elena Gayo; Olga Alarcia; Daniel Pinto; Ileana Z. Martínez; Patricia González; Ramón Balsera; Jaime L. Marcos; Ana Balseiro. Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population? Transboundary and Emerging Diseases 2018, 65, 2049 -2056.
AMA StyleJuan F. García Marín, Luis J. Royo, Alvaro Oleaga, Elena Gayo, Olga Alarcia, Daniel Pinto, Ileana Z. Martínez, Patricia González, Ramón Balsera, Jaime L. Marcos, Ana Balseiro. Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population? Transboundary and Emerging Diseases. 2018; 65 (6):2049-2056.
Chicago/Turabian StyleJuan F. García Marín; Luis J. Royo; Alvaro Oleaga; Elena Gayo; Olga Alarcia; Daniel Pinto; Ileana Z. Martínez; Patricia González; Ramón Balsera; Jaime L. Marcos; Ana Balseiro. 2018. "Canine adenovirus type 1 (CAdV-1) in free-ranging European brown bear (Ursus arctos arctos ): A threat for Cantabrian population?" Transboundary and Emerging Diseases 65, no. 6: 2049-2056.
Louping ill-like virus (LI) has been recently detected in two different locations in the north of Spain and separated by only around 400 km. Using molecular approaches, the viruses causing both outbreaks have been shown to be different to LI virus, but also different to each other. They have been called SSEV (Spanish sheep encephalitis virus) and SGEV (Spanish goat encephalitis virus) taking into account the species from which they were isolated. The aim of this paper was to design a quantitative TaqMan real-time RT-PCR protocol, for the specific diagnostic and quantitation of SGEV. Linearity, efficiency and dynamic range as well as reproducibility and specificity of the method has been tested and established. The method has proved to be valid for the specific detection and viral load quantitation of SGEV genome in virus isolates and tissue samples from infected animals. This assay will be a useful analytical tool in early diagnosis and epidemiological surveys.
Ana Del Cerro; Ana Balseiro; Rosa Casais; Kevin P. Dalton; Luis Salinas; Juan Carlos Saiz; Luis J. Royo. A one-step TaqMan real-time qRT-PCR assay for the specific detection and quantitation of the Spanish goat encephalitis virus (SGEV). Journal of Virological Methods 2018, 255, 98 -100.
AMA StyleAna Del Cerro, Ana Balseiro, Rosa Casais, Kevin P. Dalton, Luis Salinas, Juan Carlos Saiz, Luis J. Royo. A one-step TaqMan real-time qRT-PCR assay for the specific detection and quantitation of the Spanish goat encephalitis virus (SGEV). Journal of Virological Methods. 2018; 255 ():98-100.
Chicago/Turabian StyleAna Del Cerro; Ana Balseiro; Rosa Casais; Kevin P. Dalton; Luis Salinas; Juan Carlos Saiz; Luis J. Royo. 2018. "A one-step TaqMan real-time qRT-PCR assay for the specific detection and quantitation of the Spanish goat encephalitis virus (SGEV)." Journal of Virological Methods 255, no. : 98-100.
Spanish goat encephalitis virus (SGEV) is a member of the genus Flavivirus, family Flaviviridae, and causes encephalomyelitis in goats. The aim of this study was to determine whether sheep are susceptible to experimental challenge with SGEV by two different routes. The results show that SGEV can infect sheep by both the subcutaneous and intravenous routes, resulting in neurological clinical disease with extensive and severe histological lesions in the central nervous system. Lambs challenged subcutaneously developed more severe lesions on the ipsilateral side of the brain, but the lesion morphology was similar irrespective of the route of challenge. The clinical presentation, pathogenesis, lesion morphology and distribution shows that SGEV is very similar to louping ill virus (LIV) and therefore any disease control plan must take into account any host species and SGEV vectors as potential reservoirs. Furthermore, discriminatory diagnostics need to be applied to any sheep or goat suspected of disease due to any flavivirus in areas where SGEV and LIV co-exist.
L.M. Salinas; Rosa Casais; J.F. García Marín; Kevin P. Dalton; L.J. Royo; A. Del Cerro; E. Gayo; M.P. Dagleish; R.A. Juste; A. Balseiro. Lambs are Susceptible to Experimental Challenge with Spanish Goat Encephalitis Virus. Journal of Comparative Pathology 2017, 156, 400 -408.
AMA StyleL.M. Salinas, Rosa Casais, J.F. García Marín, Kevin P. Dalton, L.J. Royo, A. Del Cerro, E. Gayo, M.P. Dagleish, R.A. Juste, A. Balseiro. Lambs are Susceptible to Experimental Challenge with Spanish Goat Encephalitis Virus. Journal of Comparative Pathology. 2017; 156 (4):400-408.
Chicago/Turabian StyleL.M. Salinas; Rosa Casais; J.F. García Marín; Kevin P. Dalton; L.J. Royo; A. Del Cerro; E. Gayo; M.P. Dagleish; R.A. Juste; A. Balseiro. 2017. "Lambs are Susceptible to Experimental Challenge with Spanish Goat Encephalitis Virus." Journal of Comparative Pathology 156, no. 4: 400-408.
Spanish goat encephalitis virus (SGEV) is a recently described member of the genus Flavivirus belonging to the tick-borne encephalitis group of viruses, and is closely related to louping ill virus (LIV). Naturally acquired disease in goats results in severe, acute encephalitis and 100% mortality. Eighteen goats were challenged subcutaneously with SGEV; nine were vaccinated previously against LIV and nine were not. None of the vaccinated goats showed any clinical signs of disease or histological lesions, but all of the non-vaccinated goats developed pyrexia and 5/9 developed neurological clinical signs, primarily tremors in the neck and ataxia. All non-vaccinated animals developed histological lesions restricted to the central nervous system and consistent with a lymphocytic meningomyeloencephalitis. Vaccinated goats had significantly (P <0.003) greater concentrations of serum IgG and lower levels of IgM (P <0.0001) compared with unvaccinated animals. SGEV RNA levels were below detectable limits in the vaccinated goats throughout the experiment, but increased rapidly and were significantly (P <0.0001) greater 2-10 days post challenge in the non-vaccinated group. In conclusion, vaccination of goats against LIV confers highly effective protection against SGEV; this is probably mediated by IgG and prevents an increase in viral RNA load in serum such that vaccinated animals would not be an effective reservoir of the virus.
L.M. Salinas; Rosa Casais; J.F. García Marín; Kevin P. Dalton; L.J. Royo; A. del Cerro; E. Gayo; M.P. Dagleish; Pilar Alberdi; R.A. Juste; J. de la Fuente; A. Balseiro. Vaccination against Louping Ill Virus Protects Goats from Experimental Challenge with Spanish Goat Encephalitis Virus. Journal of Comparative Pathology 2017, 156, 409 -418.
AMA StyleL.M. Salinas, Rosa Casais, J.F. García Marín, Kevin P. Dalton, L.J. Royo, A. del Cerro, E. Gayo, M.P. Dagleish, Pilar Alberdi, R.A. Juste, J. de la Fuente, A. Balseiro. Vaccination against Louping Ill Virus Protects Goats from Experimental Challenge with Spanish Goat Encephalitis Virus. Journal of Comparative Pathology. 2017; 156 (4):409-418.
Chicago/Turabian StyleL.M. Salinas; Rosa Casais; J.F. García Marín; Kevin P. Dalton; L.J. Royo; A. del Cerro; E. Gayo; M.P. Dagleish; Pilar Alberdi; R.A. Juste; J. de la Fuente; A. Balseiro. 2017. "Vaccination against Louping Ill Virus Protects Goats from Experimental Challenge with Spanish Goat Encephalitis Virus." Journal of Comparative Pathology 156, no. 4: 409-418.
The aim of this work was to perform a thorough analysis of the diversity of Y‐haplotypes in Spanish cattle. A total of 207 Bos taurus males were sampled across 25 European breeds, with a special focus on rare, local Spanish populations. Animals were genotyped with five Y‐specific microsatellites (INRA189, UMN0103, UMN0307, BM861 and BYM1), two indels (ZFY10 and USP9Y) and one SNP (UTY19). A new haplogroup, distinct from those described by Götherström et al. (2005), was identified and named Y1.2. Samples representing the three B. taurus Y‐haplogroups were genotyped for four additional Y chromosome SNPs (rs121919254, rs121919281, rs121919323 and rs137049553). Among these SNPs, only rs121919281 was informative in B. taurus and helped to confirm the new Y1.2 haplogroup. Analysis of a larger dataset of standardized haplotypes for 1507 individuals from 57 populations from Spain, other European countries and Africa showed the new Y1.2 haplogroup to be found exclusively in Spanish breeds. This finding reinforces the importance of local Spanish cattle as reservoirs of genetic diversity as well as the importance of the Iberian Peninsula in the history of cattle.
R. Pelayo; M. C. T. Penedo; M. Valera; A. Molina; L. Millon; Catarina Ginja; L. J. Royo. Identification of a new Y chromosome haplogroup in Spanish native cattle. Animal Genetics 2017, 48, 450 -454.
AMA StyleR. Pelayo, M. C. T. Penedo, M. Valera, A. Molina, L. Millon, Catarina Ginja, L. J. Royo. Identification of a new Y chromosome haplogroup in Spanish native cattle. Animal Genetics. 2017; 48 (4):450-454.
Chicago/Turabian StyleR. Pelayo; M. C. T. Penedo; M. Valera; A. Molina; L. Millon; Catarina Ginja; L. J. Royo. 2017. "Identification of a new Y chromosome haplogroup in Spanish native cattle." Animal Genetics 48, no. 4: 450-454.
The main objective of this work was to determine whether the five founding castes defined in the Lidia cattle breed actually have an important contribution to the Spanish paternal genetic stock as well as to the paternal genetic origin support. A total of 1300 Bos taurus male individuals were genotyped for five microsatellites (INRA189, UMN0103, UMN0307, BM861 and BYM1) and one indel (ZFY10). Microsatellite and indel alleles were combined into haplotypes, identifying a total of 38 haplotypes, 11 of them belonging to haplogroup Y1 and 27 to haplogroup Y2. Ten different haplotypes were found in the Lidia cattle breed, with five being exclusive to this breed. Our results agree with different male genetic stocks in the Lidia breed: one hypothetically representing the ancient Iberian bovine genetic stock (Gallardo, Navarra and Cabrera castes and some encastes from Vistahermosa) and a second one that is the result of the more recent breeding strategy of choosing the most aggressive individuals from traditional herds (including some Vistahermosa encastes and the Vazqueña caste). In terms of conservation, it would be better to not consider this breed as a unit but to consider the caste, or even better the encaste, as the target of putative conservation efforts.
R. Pelayo; M. Valera; A. Molina; L. J. Royo. Contribution of Lidia cattle breed historical castes to the paternal genetic stock of Spain. Animal Genetics 2015, 46, 312 -315.
AMA StyleR. Pelayo, M. Valera, A. Molina, L. J. Royo. Contribution of Lidia cattle breed historical castes to the paternal genetic stock of Spain. Animal Genetics. 2015; 46 (3):312-315.
Chicago/Turabian StyleR. Pelayo; M. Valera; A. Molina; L. J. Royo. 2015. "Contribution of Lidia cattle breed historical castes to the paternal genetic stock of Spain." Animal Genetics 46, no. 3: 312-315.
Between January and June 2013, nine stillborn bovine foetuses with congenital malformations from nine cattle herds located in Salamanca (central Spain) were detected. Necropsy was performed on two calves. Pathological lesions together with molecular genetics and serological results allowed a definitive diagnosis: first confirmation of Schmallenberg virus (SBV) infection in cattle in Spain. SBV was detected in different tissues and organic fluids in both animals including blood, suggesting a possible viraemia. The umbilical cord was also positive for the presence of SBV in both animals. The former tissue provides an easy to obtain sample and might be a sample of choice when necropsy is carried out in the field.
A. Balseiro; L. J. Royo; A. Gómez Antona; J. F. García Marín. First Confirmation of Schmallenberg Virus in Cattle in Spain: Tissue Distribution and Pathology. Transboundary and Emerging Diseases 2013, 62, 1 .
AMA StyleA. Balseiro, L. J. Royo, A. Gómez Antona, J. F. García Marín. First Confirmation of Schmallenberg Virus in Cattle in Spain: Tissue Distribution and Pathology. Transboundary and Emerging Diseases. 2013; 62 (5):1.
Chicago/Turabian StyleA. Balseiro; L. J. Royo; A. Gómez Antona; J. F. García Marín. 2013. "First Confirmation of Schmallenberg Virus in Cattle in Spain: Tissue Distribution and Pathology." Transboundary and Emerging Diseases 62, no. 5: 1.
In the present study, we used high-resolution melting analysis to simultaneously identify the two mutations that taken together give us the genotype of the most common bovine β-casein variants. Allelic variants are detected in real time, and no post-PCR manipulations are required, therefore limiting costs and possible carryover contamination. Data can be copied to a Microsoft Excel spreadsheet for semiautomatic determination of the genotype. The method was validated using different DNA sources, in 122 samples from 12 different bovine populations, obtaining the first report on bovine β-casein genotypes for some of them.
Luis J. Royo; Ana del Cerro; Fernando Vicente; Alfonso Carballal; Begoña de la Roza-Delgado. An accurate high-resolution melting method to genotype bovine β-casein. European Food Research and Technology 2013, 238, 295 -298.
AMA StyleLuis J. Royo, Ana del Cerro, Fernando Vicente, Alfonso Carballal, Begoña de la Roza-Delgado. An accurate high-resolution melting method to genotype bovine β-casein. European Food Research and Technology. 2013; 238 (2):295-298.
Chicago/Turabian StyleLuis J. Royo; Ana del Cerro; Fernando Vicente; Alfonso Carballal; Begoña de la Roza-Delgado. 2013. "An accurate high-resolution melting method to genotype bovine β-casein." European Food Research and Technology 238, no. 2: 295-298.
Recent studies suggest that badgers may be a potential reservoir of Mycobacterium bovis infection for cattle in Northern Spain. The objective of this study was to investigate potential epidemiological links between cattle and badgers. Culture and molecular typing data were available for cattle culled during the national tuberculosis (TB) eradication campaigns between 2008 and 2012, as well as from 171 necropsied badgers and 60 live animals trapped and examined over the same time period. Mycobacterium tuberculosis complex strains were isolated from pooled tissues of 14 (8.2%) necropsied badgers, of which 11 were identified as M. bovis: six different spoligotypes of M. bovis were subsequently identified. In two geographical locations where these isolates were shared between cattle and badgers, infected cattle herds and badgers lived in close contact. Although it remains unclear if badgers are a maintenance or spill-over host of M. bovis in this setting, it would appear prudent to have precautionary measures in place to reduce contact between cattle and badgers
Ana Balseiro; Pablo González-Quirós; Óscar Rodríguez; M. Francisca Copano; Isabel Merediz; Lucía De Juan; Mark Chambers; Richard J. Delahay; Nelson Marreros; Luis J. Royo; Javier Bezos; José M. Prieto; Christian Gortázar. Spatial relationships between Eurasian badgers (Meles meles) and cattle infected with Mycobacterium bovis in Northern Spain. The Veterinary Journal 2013, 197, 739 -745.
AMA StyleAna Balseiro, Pablo González-Quirós, Óscar Rodríguez, M. Francisca Copano, Isabel Merediz, Lucía De Juan, Mark Chambers, Richard J. Delahay, Nelson Marreros, Luis J. Royo, Javier Bezos, José M. Prieto, Christian Gortázar. Spatial relationships between Eurasian badgers (Meles meles) and cattle infected with Mycobacterium bovis in Northern Spain. The Veterinary Journal. 2013; 197 (3):739-745.
Chicago/Turabian StyleAna Balseiro; Pablo González-Quirós; Óscar Rodríguez; M. Francisca Copano; Isabel Merediz; Lucía De Juan; Mark Chambers; Richard J. Delahay; Nelson Marreros; Luis J. Royo; Javier Bezos; José M. Prieto; Christian Gortázar. 2013. "Spatial relationships between Eurasian badgers (Meles meles) and cattle infected with Mycobacterium bovis in Northern Spain." The Veterinary Journal 197, no. 3: 739-745.
Meat is a significant source of high-quality nutrients, which are very important in the diet. Among meat products, one of the most prized is bovine meat, of which male beef has been designated to be of a higher quality. However, because of its similarity with female beef, deliberate or unintentional substitutions can occur. To avoid this, methodology based on the fast real-time polymerase chain reaction has been developed to authenticate the species and gender origin of beef. This technique consists of two polymerase chain reactions: one bovine-specific reaction and another Y-chromosome-specific multiplex reaction. This methodology has been validated for all kinds of beef products, including those subjected to intensive processing treatments, and it has subsequently been applied to 10 commercial samples labelled as ox to determine whether they are properly labelled. This assay has been shown to have high specificity, sensitivity and rapidity, with the potential to be a powerful tool in enforcing food labelling regulations.
Beatriz Herrero; Luis J. Royo; Fátima C. Lago; Juan M. Vieites; Montserrat Espiñeira. Authentication of male beef by multiplex fast real-time PCR. Food Additives & Contaminants: Part A 2013, 30, 218 -225.
AMA StyleBeatriz Herrero, Luis J. Royo, Fátima C. Lago, Juan M. Vieites, Montserrat Espiñeira. Authentication of male beef by multiplex fast real-time PCR. Food Additives & Contaminants: Part A. 2013; 30 (2):218-225.
Chicago/Turabian StyleBeatriz Herrero; Luis J. Royo; Fátima C. Lago; Juan M. Vieites; Montserrat Espiñeira. 2013. "Authentication of male beef by multiplex fast real-time PCR." Food Additives & Contaminants: Part A 30, no. 2: 218-225.
A. Balseiro; L.J. Royo; C. Pérez; M.F. Copano; T. Rubio; U. Höfle; M. Prieto; J.F. García Marín. Louping Ill in Goats in Northern Spain: More Than a Forgotten Disease. Journal of Comparative Pathology 2013, 148, 51 .
AMA StyleA. Balseiro, L.J. Royo, C. Pérez, M.F. Copano, T. Rubio, U. Höfle, M. Prieto, J.F. García Marín. Louping Ill in Goats in Northern Spain: More Than a Forgotten Disease. Journal of Comparative Pathology. 2013; 148 (1):51.
Chicago/Turabian StyleA. Balseiro; L.J. Royo; C. Pérez; M.F. Copano; T. Rubio; U. Höfle; M. Prieto; J.F. García Marín. 2013. "Louping Ill in Goats in Northern Spain: More Than a Forgotten Disease." Journal of Comparative Pathology 148, no. 1: 51.
Variation in mitochondrial DNA (mtDNA) and Y-chromosome haplotypes was analysed in nine domestic sheep breeds (159 rams) and 21 mouflon (Ovis musimon) sampled in the East Adriatic. Mitochondrial DNA analyses revealed a high frequency of type B haplotypes, predominantly in European breeds, and a very low frequency of type A haplotypes, which are more frequent in some Asian breeds. Mitochondrial haplotype Hmt-3 was the most frequent (26.4%), and 37.1%, 20.8% and 7.6% of rams had haplotypes one, two and three mutations remote from Hmt-3 respectively. In contrast, Y-chromosome analyses revealed extraordinary paternal allelic richness: HY-6, 89.3%; HY-8, 5.0%; HY-18, 3.1%; HY-7, 1.3%; and HY-5, 1.3%. In fact, the number of haplotypes observed is comparable to the number found in Turkish breeds and greater than the number found in European breeds so far. Haplotype HY-18 (A-oY1/135-SRYM18), identified here for the first time, provides a link between the haplotype HY-12 (A-oY1/139-SRYM18) found in a few rams in Turkey and haplotype HY-9 (A-oY1/131-SRYM18) found in one ram in Ethiopia. All mouflons had type B mtDNA haplotypes, including the private haplotype (Hmt-55), and all were paternally monomorphic for haplotype HY-6. Our data support a quite homogeneous maternal origin of East Adriatic sheep, which is a characteristic of European breeds. At the same time, the high number of haplotypes found was surprising and intriguing, and it begs for further analysis. Simultaneous analysis of mtDNA and Y-chromosome information allowed us to detect a large discrepancy between maternal and paternal lineages in some populations. This is most likely the result of breeder efforts to 'upgrade' local populations using rams with different paternal origins.
M. Ferencakovic; I. Curik; Lucía Pérez Pardal; L. J. Royo; Vlatka Cubric-Curik; Ivan Fernandez; Isabel Alvarez; A. Kostelic; Nikica Šprem; K. Krapinec; F. Goyache. Mitochondrial DNA and Y-chromosome diversity in East Adriatic sheep. Animal Genetics 2012, 44, 184 -192.
AMA StyleM. Ferencakovic, I. Curik, Lucía Pérez Pardal, L. J. Royo, Vlatka Cubric-Curik, Ivan Fernandez, Isabel Alvarez, A. Kostelic, Nikica Šprem, K. Krapinec, F. Goyache. Mitochondrial DNA and Y-chromosome diversity in East Adriatic sheep. Animal Genetics. 2012; 44 (2):184-192.
Chicago/Turabian StyleM. Ferencakovic; I. Curik; Lucía Pérez Pardal; L. J. Royo; Vlatka Cubric-Curik; Ivan Fernandez; Isabel Alvarez; A. Kostelic; Nikica Šprem; K. Krapinec; F. Goyache. 2012. "Mitochondrial DNA and Y-chromosome diversity in East Adriatic sheep." Animal Genetics 44, no. 2: 184-192.