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Hyung Joo Kim
Department of Biological Engineering, Konkuk University, Seoul 143701, Korea

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Journal article
Published: 30 July 2021 in Processes
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To prevent microbial growth, chemical solvents are typically utilized. However, chemical solvents are hazardous to human health with low antimicrobial effects. Metallic-element (such as copper, silver, and gold) nanoparticles have many applications in biotechnology and biomedicine. Copper nanoparticles (CuNPs) are efficient owing to their antimicrobial, anti-inflammatory, and anti-proliferative properties. The objective of this study was to perform biogenic synthesis of copper nanoparticles using three different spices (star anise, seed of Illicium verum; nutmeg, seed of Myristica fragrans; and mace, membrane covering the seed of Myristica fragrans) and determine their antibacterial properties. CuNPs of spices were prepared by dissolving copper sulfate in the respective plant extract. They were then characterized by UV-Vis spectroscopy, FTIR, GC-MS, EDAX, and SEM analysis. Results of UV-Vis spectroscopy showed the maximum absorbance peak at 350 nm. SEM analysis showed that the sizes of these CuNPs were in the range of 150–200 nm. EDAX analysis confirmed the presence of copper and oxygen and revealed that copper existed in an oxidized form. FTIR spectroscopy showed the presence of different functional groups in these synthesized nanoparticles. GC-MS analysis revealed compounds such as Anethole D-limonene, heptadecanoic acid, 16-methyl-, methyl ester, myristene, methyl eugenol, and methyl stearate, indicating the presence of functional groups. The antibacterial activities of the three extracts from spices were analyzed using growth zone inhibition and TLC-bioautography methods. The results showed that star anise spice extract had the highest antibacterial activity. These results indicate that such CuNPs phyto-formulated with spice extracts having antibacterial properties could be used as potential therapeutics for microbial diseases.

ACS Style

Gayathri Vijayakumar; Hindhuja Kesavan; Anisha Kannan; Dhanalakshmi Arulanandam; Jeong Kim; Kwang Kim; Hak Song; Hyung Kim; Senthil Rangarajulu. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes 2021, 9, 1341 .

AMA Style

Gayathri Vijayakumar, Hindhuja Kesavan, Anisha Kannan, Dhanalakshmi Arulanandam, Jeong Kim, Kwang Kim, Hak Song, Hyung Kim, Senthil Rangarajulu. Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties. Processes. 2021; 9 (8):1341.

Chicago/Turabian Style

Gayathri Vijayakumar; Hindhuja Kesavan; Anisha Kannan; Dhanalakshmi Arulanandam; Jeong Kim; Kwang Kim; Hak Song; Hyung Kim; Senthil Rangarajulu. 2021. "Phytosynthesis of Copper Nanoparticles Using Extracts of Spices and Their Antibacterial Properties." Processes 9, no. 8: 1341.

Journal article
Published: 29 May 2021 in Plant Methods
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Background Water content variation during plant growth is one of the most important monitoring parameters in plant studies. Conventional parameters (such as dry weight) are unreliable; thus, the development of rapid, accurate methods that will allow the monitoring of water content variation in live plants is necessary. In this study, we aimed to develop a non-invasive, radiofrequency-based monitoring system to rapidly and accurately detect water content variation in live plants. The changes in standing wave ratio (SWR) caused by the presence of stem water and magnetic particles in the stem water flow were used as the basis of plant monitoring systems. Results The SWR of a coil probe was used to develop a non-invasive monitoring system to detect water content variation in live plants. When water was added to the live experimental plants with or without illumination under drought conditions, noticeable SWR changes at various frequencies were observed. When a fixed frequency (1.611 GHz) was applied to a single experimental plant (Radermachera sinica), a more comprehensive monitoring, such as water content variation within the plant and the effect of illumination on water content, was achieved. Conclusions Our study demonstrated that the SWR of a coil probe could be used as a real-time, non-invasive, non-destructive parameter for detecting water content variation and practical vital activity in live plants. Our non-invasive monitoring method based on SWR may also be applied to various plant studies.

ACS Style

Yunjeong Yang; Ji Eun Kim; Hak Jin Song; Eun Bin Lee; Yong-Keun Choi; Jeong Wook Jo; Hyeon Jin Jeon; Ho Hyun Kim; Kwang Jin Kim; Hyung Joo Kim. Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants. Plant Methods 2021, 17, 1 .

AMA Style

Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim, Hyung Joo Kim. Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants. Plant Methods. 2021; 17 (1):1.

Chicago/Turabian Style

Yunjeong Yang; Ji Eun Kim; Hak Jin Song; Eun Bin Lee; Yong-Keun Choi; Jeong Wook Jo; Hyeon Jin Jeon; Ho Hyun Kim; Kwang Jin Kim; Hyung Joo Kim. 2021. "Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants." Plant Methods 17, no. 1: 1.

Journal article
Published: 29 May 2021 in Plant Methods
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Yunjeong Yang; Ji Eun Kim; Hak Jin Song; Eun Bin Lee; Yong-Keun Choi; Jeong Wook Jo; Hyeon Jin Jeon; Ho Hyun Kim; Kwang Jin Kim; Hyung Joo Kim. Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants. Plant Methods 2021, 17, 56 .

AMA Style

Yunjeong Yang, Ji Eun Kim, Hak Jin Song, Eun Bin Lee, Yong-Keun Choi, Jeong Wook Jo, Hyeon Jin Jeon, Ho Hyun Kim, Kwang Jin Kim, Hyung Joo Kim. Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants. Plant Methods. 2021; 17 (1):56.

Chicago/Turabian Style

Yunjeong Yang; Ji Eun Kim; Hak Jin Song; Eun Bin Lee; Yong-Keun Choi; Jeong Wook Jo; Hyeon Jin Jeon; Ho Hyun Kim; Kwang Jin Kim; Hyung Joo Kim. 2021. "Methodology: non-invasive monitoring system based on standing wave ratio for detecting water content variations in plants." Plant Methods 17, no. 1: 56.

Journal article
Published: 18 May 2021 in Processes
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The utilization of biological resources on the manufacture of nano silver has attracted the interest of researchers to develop an eco-friendly, cost-effective technology in nanomaterials production. In the present study, plant-mediated silver nanoparticles (AgNPs) were synthesized using aqueous leaf extracts of the Copperpod plant, which was well characterized. The ultraviolet-visible spectrophotometric study showed a maximum absorbance peak at 425 nm, and the observation of transmission electron microscopic features revealed that the nanoparticles size ranged between 20 and 70 nm. The synthesized AgNPs were tested for in vitro cytotoxic effects against cancerous cells, such as HepG2, A549 and MCF-7 cells. The findings showed that the IC50 values of AgNPs against cancerous cells viz., HepG2, MCF-7 and A549 cells, were observed to be 69 µg/mL, 62 µg/mL and 53 µg/mL, respectively. In addition, the apoptosis property was analysed using propidium iodide and acridine orange-ethidium bromide via the DNA fragmentation technique. Thus, the outcomes of the current analysis presume that the plant mediated AgNPs obtained from a synthesized Copperpod plant possess significant anti-cancer properties against various cancerous cells.

ACS Style

Balashanmugam Pannerselvam; Devasena Thiyagarajan; Annamalai Pazhani; Kalaichelvan Thangavelu; Hyung Kim; Senthil Rangarajulu. Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines. Processes 2021, 9, 888 .

AMA Style

Balashanmugam Pannerselvam, Devasena Thiyagarajan, Annamalai Pazhani, Kalaichelvan Thangavelu, Hyung Kim, Senthil Rangarajulu. Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines. Processes. 2021; 9 (5):888.

Chicago/Turabian Style

Balashanmugam Pannerselvam; Devasena Thiyagarajan; Annamalai Pazhani; Kalaichelvan Thangavelu; Hyung Kim; Senthil Rangarajulu. 2021. "Copperpod Plant Synthesized AgNPs Enhance Cytotoxic and Apoptotic Effect in Cancer Cell Lines." Processes 9, no. 5: 888.

Journal article
Published: 06 April 2021 in Journal of Water Process Engineering
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The present study revealed adsorption behaviors of microcystin-LR (MC-LR) on pristine biochars (BCs) derived from various waste biomass of Kentucky bluegrass (KB) (Poa pratensis L.), microalgae (Spirulina sp.), grape pomace, coffee residues, and rice husk. BCs obtained from waste biomass on pyrolysis at 350, 550, and 750 °C for 2 h demonstrated different physicochemical characteristics. The maximum adsorption capacity of 2769 μg MC-LR/g was observed in KB biochar prepared at 750 °C (KB-BC750) with the involvement of hydrophobic and π-π interaction, electrostatic attraction, ion exchange, and surface complexation as the principal adsorption mechanisms. MC-LR adsorption onto KB- fitted well to Elovich kinetic and Freundlich isotherm model or the sorption. Furthermore, MC-LR adsorption from the real wastewater using KB-BC750 demonstrated significant removal (ca. 50 %) as compared to the distilled water. Therefore, the results of the present study have heightened the strategies to remove MC-LR from the wastewater using cheap adsorbents like KB-BCs.

ACS Style

Hak Jin Song; Ranjit Gurav; Shashi Kant Bhatia; Eun Bin Lee; Hyung Joo Kim; Yung-Hun Yang; Eunsung Kan; Ho Hyun Kim; Sang Hyun Lee; Yong-Keun Choi. Treatment of microcystin-LR cyanotoxin contaminated water using Kentucky bluegrass-derived biochar. Journal of Water Process Engineering 2021, 41, 102054 .

AMA Style

Hak Jin Song, Ranjit Gurav, Shashi Kant Bhatia, Eun Bin Lee, Hyung Joo Kim, Yung-Hun Yang, Eunsung Kan, Ho Hyun Kim, Sang Hyun Lee, Yong-Keun Choi. Treatment of microcystin-LR cyanotoxin contaminated water using Kentucky bluegrass-derived biochar. Journal of Water Process Engineering. 2021; 41 ():102054.

Chicago/Turabian Style

Hak Jin Song; Ranjit Gurav; Shashi Kant Bhatia; Eun Bin Lee; Hyung Joo Kim; Yung-Hun Yang; Eunsung Kan; Ho Hyun Kim; Sang Hyun Lee; Yong-Keun Choi. 2021. "Treatment of microcystin-LR cyanotoxin contaminated water using Kentucky bluegrass-derived biochar." Journal of Water Process Engineering 41, no. : 102054.

Journal article
Published: 04 November 2020 in Environmental Technology & Innovation
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In this work, the adsorptive behaviors and mechanisms of grape pomace-derived biochar (GP-BC) were assessed for the removal of the pesticide cymoxanil (CM). The biochars were produced via pyrolysis carbonization at different temperatures (350, 550, and 750 °C) and batch adsorption experiments were conducted to determine various parameters, such as pyrolytic temperature, initial solution pH, and kinetic and isotherm models. The biochar produced at low pyrolytic temperature (i.e., 350 °C) exhibited lower surface area (0.25 m2/g), and high K (1.94%) and H/C (0.905) content. The maximum cymoxanil adsorption capacity of 161 mg CM/g BC at pH 7 was achieved in biochar produced at 350 °C. Consequently, cymoxanil adsorption was attributed to the combined influences of metal and hydrophilic interaction. In addition, pseudo-second order kinetics and the Freundlich isotherm model were the best fit for cymoxanil adsorption, indicating chemical sorption and multilayer formation on the heterogeneous surface of biochar as adsorptive mechanisms. Our findings imply that GP-BC is a promising adsorbent for pesticide treatment and such an application creates an excellent closed-loop system.

ACS Style

Jae-Yoon Yoon; Ji Eun Kim; Hak Jin Song; Kyung Bin Oh; Jeong Wook Jo; Yung-Hun Yang; Sang Hyun Lee; Gwangnam Kang; Hyung Joo Kim; Yong-Keun Choi. Assessment of adsorptive behaviors and properties of grape pomace-derived biochar as adsorbent for removal of cymoxanil pesticide. Environmental Technology & Innovation 2020, 21, 101242 .

AMA Style

Jae-Yoon Yoon, Ji Eun Kim, Hak Jin Song, Kyung Bin Oh, Jeong Wook Jo, Yung-Hun Yang, Sang Hyun Lee, Gwangnam Kang, Hyung Joo Kim, Yong-Keun Choi. Assessment of adsorptive behaviors and properties of grape pomace-derived biochar as adsorbent for removal of cymoxanil pesticide. Environmental Technology & Innovation. 2020; 21 ():101242.

Chicago/Turabian Style

Jae-Yoon Yoon; Ji Eun Kim; Hak Jin Song; Kyung Bin Oh; Jeong Wook Jo; Yung-Hun Yang; Sang Hyun Lee; Gwangnam Kang; Hyung Joo Kim; Yong-Keun Choi. 2020. "Assessment of adsorptive behaviors and properties of grape pomace-derived biochar as adsorbent for removal of cymoxanil pesticide." Environmental Technology & Innovation 21, no. : 101242.

Journal article
Published: 25 April 2020 in Applied Sciences
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Rapid development in the printing and dying industry produces large amounts of wastewater, and its discharge in the environment causes pollution. Keeping in view the carcinogenic and mutagenic properties of various dyes, it is important to treat dyed wastewater. Maple leaf biochars were produced at different pyrolysis temperatures, i.e., 350 °C, 550 °C, and 750 °C, characterized for physicochemical properties and used for the removal of cationic (methylene blue (MB)) and anionic dye (congo red (CR)). Response surface methodology (RSM) using three variables, i.e., pH (4, 7, and 10), pyrolysis temperature (350 °C, 550 °C, and 750 °C), and adsorption temperature (20 °C, 30 °C, and 40 °C), was designed to find the optimum condition for dyes removal. X-ray diffraction (XRD) analysis showed an increase in CaCO3 crystallinity and a decrease in MgCO3 crystallinity with the increase of pyrolysis temperature. RSM design results showed that maple biochar showed maximum adsorption capacity for cationic dye at higher pH (9–10) and for anionic dye at pH 4-6, respectively. Under the selected condition of pH 7 and an adsorption temperature of 30 °C, biochar MB550 was able to remove MB and CR by 68% and 74%, respectively, from dye mixtures. Fourier transform infrared (FTIR) and X-ray photoelectron spectroscopy (XPS) analyses showed that MB550 was able to remove both dyes simultaneously from the aqueous mixtures.

ACS Style

Yong-Keun Choi; Ranjit Gurav; Hyung Joo Kim; Yung-Hun Yang; Shashi Kant Bhatia. Evaluation for Simultaneous Removal of Anionic and Cationic Dyes onto Maple Leaf-Derived Biochar Using Response Surface Methodology. Applied Sciences 2020, 10, 2982 .

AMA Style

Yong-Keun Choi, Ranjit Gurav, Hyung Joo Kim, Yung-Hun Yang, Shashi Kant Bhatia. Evaluation for Simultaneous Removal of Anionic and Cationic Dyes onto Maple Leaf-Derived Biochar Using Response Surface Methodology. Applied Sciences. 2020; 10 (9):2982.

Chicago/Turabian Style

Yong-Keun Choi; Ranjit Gurav; Hyung Joo Kim; Yung-Hun Yang; Shashi Kant Bhatia. 2020. "Evaluation for Simultaneous Removal of Anionic and Cationic Dyes onto Maple Leaf-Derived Biochar Using Response Surface Methodology." Applied Sciences 10, no. 9: 2982.

Journal article
Published: 04 April 2020 in Processes
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Cancer is one of the most dangerous threats to human health and possibly the utmost task for current medicine. Currently, bio-based synthesis of nanoparticles from plants has gained much interest due to its potential medicinal applications. In the present study, a biological approach was employed for biogenic (green) synthesis of silver nanoparticles (AgNPs) using dried leaf extract of Asian spider flower (Asf). The biogenic synthesis of Asf-AgNPs (Asian spider flower-Silver nanoparticles) was established using ultra violet-visible (UV-vis) spectra which exhibited a wide superficial plasmon resonance of AgNPs at 445 nm. These nanoparticles clearly showed the formation of poly-disperse crystalline solids (spherical shape) with particle size range of

ACS Style

Balashanmugam Pannerselvam; Prabhu Durai; Devasena Thiyagarajan; Hak Jin Song; Kwang Jin Kim; Yun Seok Jung; Hyung Joo Kim; Senthil Kumaran Rangarajulu. Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells. Processes 2020, 8, 430 .

AMA Style

Balashanmugam Pannerselvam, Prabhu Durai, Devasena Thiyagarajan, Hak Jin Song, Kwang Jin Kim, Yun Seok Jung, Hyung Joo Kim, Senthil Kumaran Rangarajulu. Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells. Processes. 2020; 8 (4):430.

Chicago/Turabian Style

Balashanmugam Pannerselvam; Prabhu Durai; Devasena Thiyagarajan; Hak Jin Song; Kwang Jin Kim; Yun Seok Jung; Hyung Joo Kim; Senthil Kumaran Rangarajulu. 2020. "Facile Synthesis of Silver Nanoparticles Using Asian Spider Flower and Its In Vitro Cytotoxic Activity Against Human Breast Carcinoma Cells." Processes 8, no. 4: 430.

Journal article
Published: 27 December 2019 in Asian Journal of Beauty and Cosmetology
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Young Whan Sung; Min Ju Chang; Yong-Keun Choi; Hakjin Song; Sok Young Shim; Jae-Yoon Yoon; Kwang Jin Kim; Hyung Joo Kim. Seasonal Variation of Anti-oxidant and Anti-bacterial Activities in Acer palmatum Extract. Asian Journal of Beauty and Cosmetology 2019, 17, 421 -430.

AMA Style

Young Whan Sung, Min Ju Chang, Yong-Keun Choi, Hakjin Song, Sok Young Shim, Jae-Yoon Yoon, Kwang Jin Kim, Hyung Joo Kim. Seasonal Variation of Anti-oxidant and Anti-bacterial Activities in Acer palmatum Extract. Asian Journal of Beauty and Cosmetology. 2019; 17 (4):421-430.

Chicago/Turabian Style

Young Whan Sung; Min Ju Chang; Yong-Keun Choi; Hakjin Song; Sok Young Shim; Jae-Yoon Yoon; Kwang Jin Kim; Hyung Joo Kim. 2019. "Seasonal Variation of Anti-oxidant and Anti-bacterial Activities in Acer palmatum Extract." Asian Journal of Beauty and Cosmetology 17, no. 4: 421-430.

Note
Published: 15 October 2019 in Bulletin of the Korean Chemical Society
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ACS Style

Yunjeong Yang; Eun Bin Lee; Ji Eun Kim; Hak Jin Song; Yong‐Keun Choi; Kwang Jin Kim; Rangarajulu Senthil Kumaran; Sang Hyun Lee; Yung‐Hun Yang; Hyung Joo Kim. Monitoring Plant Moisture Content Using an Induction Coil Sensor. Bulletin of the Korean Chemical Society 2019, 40, 1138 -1141.

AMA Style

Yunjeong Yang, Eun Bin Lee, Ji Eun Kim, Hak Jin Song, Yong‐Keun Choi, Kwang Jin Kim, Rangarajulu Senthil Kumaran, Sang Hyun Lee, Yung‐Hun Yang, Hyung Joo Kim. Monitoring Plant Moisture Content Using an Induction Coil Sensor. Bulletin of the Korean Chemical Society. 2019; 40 (11):1138-1141.

Chicago/Turabian Style

Yunjeong Yang; Eun Bin Lee; Ji Eun Kim; Hak Jin Song; Yong‐Keun Choi; Kwang Jin Kim; Rangarajulu Senthil Kumaran; Sang Hyun Lee; Yung‐Hun Yang; Hyung Joo Kim. 2019. "Monitoring Plant Moisture Content Using an Induction Coil Sensor." Bulletin of the Korean Chemical Society 40, no. 11: 1138-1141.

Journal article
Published: 01 August 2019 in Journal of Korean Society of Environmental Engineers
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Ji Eun Kim; Eun Bin Lee; Yun Jeong Yang; Woo Hyeong Kim; Jung Min Oh; Dae Yong Lim; Ye Darm Park; Min Suk Choi; Chae Young Lee; Yeon Soo An; Hak Jin Song; Yoon Jae Yoon; Hyung Joo Kim. Isolation, Counting and Identification of Microorganisms from Elevator Button, ATM, and Smartphone Surface. Journal of Korean Society of Environmental Engineers 2019, 41, 419 -430.

AMA Style

Ji Eun Kim, Eun Bin Lee, Yun Jeong Yang, Woo Hyeong Kim, Jung Min Oh, Dae Yong Lim, Ye Darm Park, Min Suk Choi, Chae Young Lee, Yeon Soo An, Hak Jin Song, Yoon Jae Yoon, Hyung Joo Kim. Isolation, Counting and Identification of Microorganisms from Elevator Button, ATM, and Smartphone Surface. Journal of Korean Society of Environmental Engineers. 2019; 41 (8):419-430.

Chicago/Turabian Style

Ji Eun Kim; Eun Bin Lee; Yun Jeong Yang; Woo Hyeong Kim; Jung Min Oh; Dae Yong Lim; Ye Darm Park; Min Suk Choi; Chae Young Lee; Yeon Soo An; Hak Jin Song; Yoon Jae Yoon; Hyung Joo Kim. 2019. "Isolation, Counting and Identification of Microorganisms from Elevator Button, ATM, and Smartphone Surface." Journal of Korean Society of Environmental Engineers 41, no. 8: 419-430.

Article
Published: 09 September 2018 in Bulletin of the Korean Chemical Society
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A plant health monitoring method based on a plant microbial fuel cell (PMFC) technique was investigated. The reducing action of plant exudates on electrochemically active bacteria (EAB) in the rhizosphere was used as the basis of the method. Ardisia pusilla was used as an experimental plant. The results indicated that the open circuit potential (OCP) development from the PMFC is closely related to plant health and affected by the cultivation environment. Distinctive OCP developments were observed using the PMFC with live, dead (heat shocked), or no plant. Cultivation conditions such as watering, fertilizing, temperature variation, and lighting affected OCP development from the PMFC. Plant root viability analysis indicated that OCP development from the PMFC is closely related to plant health. These results suggest that this method based on the PMFC technique is a facile, cost‐effective, and rapid tool for monitoring plant health.

ACS Style

Tae Hang Yoon; Hak Jin Song; Woo Young Jung; Ji Eun Kim; Kwang Jin Kim; Ho Hyun Kim; Rangarajulu Senthil Kumaran; Sunghyun Kim; Hyung Joo Kim. Monitoring Plant Health Using a Plant Microbial Fuel Cell. Bulletin of the Korean Chemical Society 2018, 39, 1193 -1197.

AMA Style

Tae Hang Yoon, Hak Jin Song, Woo Young Jung, Ji Eun Kim, Kwang Jin Kim, Ho Hyun Kim, Rangarajulu Senthil Kumaran, Sunghyun Kim, Hyung Joo Kim. Monitoring Plant Health Using a Plant Microbial Fuel Cell. Bulletin of the Korean Chemical Society. 2018; 39 (10):1193-1197.

Chicago/Turabian Style

Tae Hang Yoon; Hak Jin Song; Woo Young Jung; Ji Eun Kim; Kwang Jin Kim; Ho Hyun Kim; Rangarajulu Senthil Kumaran; Sunghyun Kim; Hyung Joo Kim. 2018. "Monitoring Plant Health Using a Plant Microbial Fuel Cell." Bulletin of the Korean Chemical Society 39, no. 10: 1193-1197.

Journal article
Published: 01 March 2018 in Nanoscience and Nanotechnology Letters
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The synthesis of metallic nanoparticles using plant extracts has attracted much attention. In this study, silver nanoparticles (AgNPs) were synthesized using the Ginkgo biloba plant-leaf extract and their antibacterial and larvicidal activities were investigated. AgNPs were characterized using UV-visible, X-ray diffraction (XRD), Fourier transform infrared (FTIR), energy-dispersive X-ray (EDX) spectroscopy and field emission scanning electron microscope (FESEM) analysis. The UV-visible spectral analysis showed a surface plasmon resonance (SPR) peak at 430 nm, the FESEM analysis revealed size of AgNPs between 25–45 nm and the XRD data confirmed the formation of the AgNPs using G. biloba-mediated green synthesis. AgNPs showed the highest antibacterial activities against Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtills, Enterobacter aerogens, Salmonella paratyphi and Escherichia coli. Also, the AgNPs exerted a significant larvicidal effect on the Culex mosquito larvae.

ACS Style

Pannerselvam Balashanmugam; Hyung Joo Kim; Vijay Singh; Rangarajulu Senthil Kumaran. Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects. Nanoscience and Nanotechnology Letters 2018, 10, 422 -428.

AMA Style

Pannerselvam Balashanmugam, Hyung Joo Kim, Vijay Singh, Rangarajulu Senthil Kumaran. Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects. Nanoscience and Nanotechnology Letters. 2018; 10 (3):422-428.

Chicago/Turabian Style

Pannerselvam Balashanmugam; Hyung Joo Kim; Vijay Singh; Rangarajulu Senthil Kumaran. 2018. "Green Synthesis of Silver Nanoparticles Using Ginkgo biloba and Their Bactericidal and Larvicidal Effects." Nanoscience and Nanotechnology Letters 10, no. 3: 422-428.

Note
Published: 03 November 2017 in Bulletin of the Korean Chemical Society
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Min Ju Chang; Chang Joong Kim; Yong-Keun Choi; Hak Jin Song; Sok Young Shim; Sang Hyun Lee; Yung-Hun Yang; Kwang Jin Kim; Hyung Joo Kim. Effect of Electrical Ground Connection on Plant Growth. Bulletin of the Korean Chemical Society 2017, 38, 1491 -1494.

AMA Style

Min Ju Chang, Chang Joong Kim, Yong-Keun Choi, Hak Jin Song, Sok Young Shim, Sang Hyun Lee, Yung-Hun Yang, Kwang Jin Kim, Hyung Joo Kim. Effect of Electrical Ground Connection on Plant Growth. Bulletin of the Korean Chemical Society. 2017; 38 (12):1491-1494.

Chicago/Turabian Style

Min Ju Chang; Chang Joong Kim; Yong-Keun Choi; Hak Jin Song; Sok Young Shim; Sang Hyun Lee; Yung-Hun Yang; Kwang Jin Kim; Hyung Joo Kim. 2017. "Effect of Electrical Ground Connection on Plant Growth." Bulletin of the Korean Chemical Society 38, no. 12: 1491-1494.

Short communication
Published: 15 March 2017 in Engineering in Life Sciences
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Microalgae are gaining importance as a source of high‐value bioproducts. However, data regarding optimization of algal productivity via variation of environmental factors are lacking. Here, we evaluated a novel lighting method for the enhancement of biomass and total fatty acid (TFA) productivities during algal cultivation. We cultivated six different algal strains (Chlorella vulgaris KCTC AG10002, Acutodesmus obliquus KGE18, Uronema sp. KGE03, Micractinium reisseri KGE19, Fragilaria sp., and Spirogyra sp.) under various lighting conditions—continuous light (CL), light‐dark cycle (LD), and continuous dark (CD)—with or without additional flashing light. We monitored dry cell weight (DCW) and TFA concentrations during cultivation. For each algal strain, the growth rate showed markedly different responses to the various lighting modes. The growth rates of C. vulgaris KCTC AG10002 (1.34‐fold DCW increase, LD with flash), A. obliquus KGE18 (5.16‐fold DCW increase, LD with flash), Uronema sp. KGE03 (2.77‐fold DCW increase, CL with flash), and M. reisseri KGE19 (1.52‐fold DCW increase, CL with flash) markedly increased in response to flashing light. Additionally, in some algal strains cultivated under the LD mode, the flashing light treatment induced increased TFA concentrations (C. vulgaris, 1.19‐fold increase; A. obliquus, 2.59‐fold increase; and M. reisseri, 3.31‐fold increase). Phytohormone analysis of M. reisseri revealed increases in growth rate and TFA concentrations, associated with phytohormone induction via flashing light (e.g. 2.93‐fold increase in gibberellic acid); hence, flashing light can promote substantial alterations in algal metabolism.

ACS Style

Yong-Keun Choi; Hyun-Joong Kim; Rangarajulu Senthil Kumaran; Hak-Jin Song; Kyung-Guen Song; Kwang Jin Kim; Sang Hyun Lee; Yung-Hun Yang; Hyung Joo Kim. Enhanced growth and total fatty acid production of microalgae under various lighting conditions induced by flashing light. Engineering in Life Sciences 2017, 17, 976 -980.

AMA Style

Yong-Keun Choi, Hyun-Joong Kim, Rangarajulu Senthil Kumaran, Hak-Jin Song, Kyung-Guen Song, Kwang Jin Kim, Sang Hyun Lee, Yung-Hun Yang, Hyung Joo Kim. Enhanced growth and total fatty acid production of microalgae under various lighting conditions induced by flashing light. Engineering in Life Sciences. 2017; 17 (9):976-980.

Chicago/Turabian Style

Yong-Keun Choi; Hyun-Joong Kim; Rangarajulu Senthil Kumaran; Hak-Jin Song; Kyung-Guen Song; Kwang Jin Kim; Sang Hyun Lee; Yung-Hun Yang; Hyung Joo Kim. 2017. "Enhanced growth and total fatty acid production of microalgae under various lighting conditions induced by flashing light." Engineering in Life Sciences 17, no. 9: 976-980.

Journal article
Published: 01 February 2017 in Carbohydrate Polymers
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Alginate/bacterial cellulose nanocomposite beads, with well-controlled size and regular spherical shapes, were prepared in a simple manner by entrapping Gluconacetobacter xylinus in barium alginate hydrogel beads, followed by cultivation of the entrapped cells in culture media with a low sodium ion concentration. The entire surface of the alginate hydrogel beads containing the cells was covered with cellulose fibers (∼30nm) after 36h of cultivation. The cellulose crystallinity index of the alginate/bacterial cellulose beads was 0.7, which was slightly lower than that of bacterial cellulose prepared by cultivating dispersed cells. The water vapor sorption capacity of the alginate/bacterial cellulose beads increased significantly from 0.07 to 38.00 (g/g dry bead) as cultivation time increased. These results clearly indicate that alginate/bacterial cellulose beads have a much higher surface area, crystallinity, and water-holding capacity than alginate beads. The immobilization of lipase on the surface of the nanocomposite beads was also investigated as a potential application of this system. The activity and specific activity of lipase immobilized on alginate/bacterial cellulose beads were 2.6- and 3.8-fold higher, respectively, than that of lipase immobilized on cellulose beads. The alginate/bacterial cellulose nanocomposite beads prepared in this study have several potential applications in the biocatalytic, biomedical, and pharmaceutical fields because of their biocompatibility, biodegradability, high crystallinity, and large surface area.

ACS Style

Ji Hyun Kim; Saerom Park; Hyungsup Kim; Hyung Joo Kim; Yung-Hun Yang; Yong Hwan Kim; Sang-Kyu Jung; Eunsung Kan; Sang Hyun Lee. Alginate/bacterial cellulose nanocomposite beads prepared using Gluconacetobacter xylinus and their application in lipase immobilization. Carbohydrate Polymers 2017, 157, 137 -145.

AMA Style

Ji Hyun Kim, Saerom Park, Hyungsup Kim, Hyung Joo Kim, Yung-Hun Yang, Yong Hwan Kim, Sang-Kyu Jung, Eunsung Kan, Sang Hyun Lee. Alginate/bacterial cellulose nanocomposite beads prepared using Gluconacetobacter xylinus and their application in lipase immobilization. Carbohydrate Polymers. 2017; 157 ():137-145.

Chicago/Turabian Style

Ji Hyun Kim; Saerom Park; Hyungsup Kim; Hyung Joo Kim; Yung-Hun Yang; Yong Hwan Kim; Sang-Kyu Jung; Eunsung Kan; Sang Hyun Lee. 2017. "Alginate/bacterial cellulose nanocomposite beads prepared using Gluconacetobacter xylinus and their application in lipase immobilization." Carbohydrate Polymers 157, no. : 137-145.

Journal article
Published: 01 October 2016 in Process Biochemistry
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Cadaverine is a useful chemical that can be produced by lysine decarboxylase up to molar concentration levels. To develop a convenient and reusable production process, we performed intracellular immobilization of lysine decarboxylase (CadA) using poly(3-hydroxybutyrate) (P(3HB)) and PhaP1 (P(3HB) granule-associated protein) from Ralstonia eutropha. By adding 591 bp of the entire phaP1 gene sequence to the 3′ end of the cadA gene, CadA was successfully fused to PhaP1. The phasin-fused CadA bound to the intracellular P(3HB) granules, which enabled the reuse of CadA in repetitive enzyme reactions. Although immobilization of the CadA–P(3HB) complex was not effective over extended temperature and pH ranges, the immobilized CadA exhibited increased thermal stability, with a half-life of 70 h at 50 °C. The CadA–P(3HB) complex achieved a 75–80% conversion yield over five reaction cycles without laborious immobilization steps. This study indicates the feasibility of in situ immobilization of lysine decarboxylase by phasin fusion.

ACS Style

Hyung-Min Seo; Jung-Ho Kim; Jong-Min Jeon; Hun-Suk Song; Shashi Kant Bhatia; Ganesan Sathiyanarayanan; Kyungmoon Park; Kwang Jin Kim; Sang Hyun Lee; Hyung Joo Kim; Yung-Hun Yang. In situ immobilization of lysine decarboxylase on a biopolymer by fusion with phasin. Process Biochemistry 2016, 51, 1413 -1419.

AMA Style

Hyung-Min Seo, Jung-Ho Kim, Jong-Min Jeon, Hun-Suk Song, Shashi Kant Bhatia, Ganesan Sathiyanarayanan, Kyungmoon Park, Kwang Jin Kim, Sang Hyun Lee, Hyung Joo Kim, Yung-Hun Yang. In situ immobilization of lysine decarboxylase on a biopolymer by fusion with phasin. Process Biochemistry. 2016; 51 (10):1413-1419.

Chicago/Turabian Style

Hyung-Min Seo; Jung-Ho Kim; Jong-Min Jeon; Hun-Suk Song; Shashi Kant Bhatia; Ganesan Sathiyanarayanan; Kyungmoon Park; Kwang Jin Kim; Sang Hyun Lee; Hyung Joo Kim; Yung-Hun Yang. 2016. "In situ immobilization of lysine decarboxylase on a biopolymer by fusion with phasin." Process Biochemistry 51, no. 10: 1413-1419.

Journal article
Published: 01 June 2016 in Journal of Molecular Catalysis B: Enzymatic
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DESs (deep eutectic solvents) have many potential applications as cosolvents or anhydrous reaction media for biocatalytic reactions, owing to their non-volatility, non-flammability, non-toxicity, biocompatibility, biodegradability, and low cost. In this work, choline chloride ([Ch]Cl)-based DESs and DES mixtures containing two hydrogen bond donors were used as cosolvents to enhance the activity and stability of Candida rugosa lipase in aqueous reactions. The activity of lipase in an aqueous solution of [Ch]Cl:urea:glycerol was 155% higher than that in buffer. The half-life time of lipase at 40 °C in an aqueous solution of [Ch]Cl:glycerol was enhanced by 9.2 times. The lipase showed the highest acid stability and base stability in the aqueous solutions of [Ch]Cl:glycerol:thiourea and [Ch]Cl:ethylene glycol:formamide, respectively. In general, glycerol-containing DES mixtures were very useful in enhancing the activity and stability of lipase, while formamide-containing DES mixtures could not efficiently enhance the activity and stability of lipase. To understand the effect of DES mixtures on the activity and stability of lipase in aqueous solution, four solvatochromic parameters of DES mixtures were determined. When the solvatochromic parameters of DES mixtures were correlated with the stability of lipase in aqueous solutions of DES mixtures, it was found that thermal stability and storage stability of lipase were associated with the hydrogen bond acidity of DES mixtures. Acid stability and base stability of lipase were correlated with polarity based on Reichardt's dye and the dipolarity/polarizability of DES mixtures, respectively.

ACS Style

Sung Hee Kim; Saerom Park; Hyejeong Yu; Ji Hyun Kim; Hyung Joo Kim; Yung-Hun Yang; Yong Hwan Kim; Kwang Jin Kim; Eunsung Kan; Sang Hyun Lee. Effect of deep eutectic solvent mixtures on lipase activity and stability. Journal of Molecular Catalysis B: Enzymatic 2016, 128, 65 -72.

AMA Style

Sung Hee Kim, Saerom Park, Hyejeong Yu, Ji Hyun Kim, Hyung Joo Kim, Yung-Hun Yang, Yong Hwan Kim, Kwang Jin Kim, Eunsung Kan, Sang Hyun Lee. Effect of deep eutectic solvent mixtures on lipase activity and stability. Journal of Molecular Catalysis B: Enzymatic. 2016; 128 ():65-72.

Chicago/Turabian Style

Sung Hee Kim; Saerom Park; Hyejeong Yu; Ji Hyun Kim; Hyung Joo Kim; Yung-Hun Yang; Yong Hwan Kim; Kwang Jin Kim; Eunsung Kan; Sang Hyun Lee. 2016. "Effect of deep eutectic solvent mixtures on lipase activity and stability." Journal of Molecular Catalysis B: Enzymatic 128, no. : 65-72.

Journal article
Published: 01 June 2016 in Biochemical Engineering Journal
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This study combined an algae treatment with post-ozonation for the robust treatment of piggery effluent in which high levels of organic constituents, inorganic nutrients, color, and salts remained. Due to a nearly complete light limitation resulting from a high level of color, the algae treatment was conducted with continuous O2 supplementation instead of using the combination of high lighting and CO2 injection. The microalga Acutodesmus obliquus KGE-17 showed tolerance to high salinity (up to 5.2% as chloride) and was capable of utilizing dissolved organics (1923 mg-COD/(g-cell)(day)) under the heterotrophic growth conditions. The use of A. obliquus for remediation of piggery effluent also resulted in an operational simplicity in the simultaneous removal of nitrogen and phosphorus in a single treatment process, in contrast to conventional biological nutrient removal processes. Subsequent ozonation was successful in decolorizing the algae-treated wastewater and in improving the biodegradability of residual organics in post-treatment processes.

ACS Style

Hyun-Chul Kim; Wook Jin Choi; A. Na Chae; Joonhong Park; Hyung Joo Kim; Kyung Guen Song. Treating high-strength saline piggery wastewater using the heterotrophic cultivation of Acutodesmus obliquus. Biochemical Engineering Journal 2016, 110, 51 -58.

AMA Style

Hyun-Chul Kim, Wook Jin Choi, A. Na Chae, Joonhong Park, Hyung Joo Kim, Kyung Guen Song. Treating high-strength saline piggery wastewater using the heterotrophic cultivation of Acutodesmus obliquus. Biochemical Engineering Journal. 2016; 110 ():51-58.

Chicago/Turabian Style

Hyun-Chul Kim; Wook Jin Choi; A. Na Chae; Joonhong Park; Hyung Joo Kim; Kyung Guen Song. 2016. "Treating high-strength saline piggery wastewater using the heterotrophic cultivation of Acutodesmus obliquus." Biochemical Engineering Journal 110, no. : 51-58.

Journal article
Published: 01 January 2016 in Journal of Nanoscience and Nanotechnology
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In the present investigation, engineered ZnO nanoparticles were tested for their induced oxidative stress in T47D tumor cell lines. The expressions of reactive oxygen species (ROS) related genes, glutathione S-transferase (GST) and catalase were quantified by real time-polymerase chain reaction (RT-PCR). In addition, qualitative analysis of GST was also performed at the cell level using molecular beacon (MB) technology. The tested nanoparticles were 20 nm in size, water-dispersible and treated on human breast tumor epithelial cell lines at 20, 40, 80 µg/ml concentration with 14, 28, 48 h incubation times. Nanoparticles induced expressions of ROS responsive genes at molecular and cellular level, produces consistent results with respect to different dosage and incubation time. The experiment showed that the expression of both GST and catalase genes were maximized at 28 h with 80 µg/ml concentration. However, the toxic effect of the monodisperse ZnO nanoparticles was not significant compared with control experiments, demonstrating its high potential in the applications of nanomedicines for a diagnostic and therapeutic tool.

ACS Style

Rangarajulu Senthil Kumaran; Yong-Keun Choi; Vijay Singh; Kwang Jin Kim; Hyung Joo Kim; Kumaran Rangarajulu Senthil; Choi Yong-Keun; Singh Vijay; Kim Kwang Jin; Kim Hyung Joo. Cytotoxic Effects of ZnO Nanoparticles on the Expression of ROS-Responsive Genes in the Human Cell Lines. Journal of Nanoscience and Nanotechnology 2016, 16, 210 -218.

AMA Style

Rangarajulu Senthil Kumaran, Yong-Keun Choi, Vijay Singh, Kwang Jin Kim, Hyung Joo Kim, Kumaran Rangarajulu Senthil, Choi Yong-Keun, Singh Vijay, Kim Kwang Jin, Kim Hyung Joo. Cytotoxic Effects of ZnO Nanoparticles on the Expression of ROS-Responsive Genes in the Human Cell Lines. Journal of Nanoscience and Nanotechnology. 2016; 16 (1):210-218.

Chicago/Turabian Style

Rangarajulu Senthil Kumaran; Yong-Keun Choi; Vijay Singh; Kwang Jin Kim; Hyung Joo Kim; Kumaran Rangarajulu Senthil; Choi Yong-Keun; Singh Vijay; Kim Kwang Jin; Kim Hyung Joo. 2016. "Cytotoxic Effects of ZnO Nanoparticles on the Expression of ROS-Responsive Genes in the Human Cell Lines." Journal of Nanoscience and Nanotechnology 16, no. 1: 210-218.