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Dr. Jorge Hernández-Bello
Instituto de Investigación en Ciencias Biomédicas, Centro Universitario de Ciencias dela Salud, Universidad de Guadalajara

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0 Immunogenetics
0 COVID 19
0 rheumaology and immunology
0 Autoinmune disease
0 B Cells & Antibodies

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Journal article
Published: 26 August 2021 in International Journal of Environmental Research and Public Health
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Developing countries have reported lower molecular diagnostic testing levels due to a lack of resources. Therefore, antibody tests represent an alternative to detect exposure to SARS-CoV-2 and analyze possible risk factors. We aimed to describe and compare the clinical-epidemiological characteristics and the quality of food intake in Mexican individuals with a positive or negative test to antibodies against SARS-CoV-2. We carried out antibody tests and applied a survey to 1799 individuals; 42% were positive, and diabetes was more prevalent in these cases (p< 0.01). No differences were identified in the blood type nor influenza vaccination between groups. Coughing, respiratory distress, muscle pain, joint pain, and anosmia were the most prevalent symptoms among seropositive cases (p< 0.0001). Food intake quality was similar in both groups, except for the most consumed type of fat (p = 0.006). In conclusion, this study supports the association of diabetes as a principal risk factor for SARS-CoV-2 infection in the Mexican population. The results do not support previous associations between blood group or influenza vaccination as protective factors against SARS-CoV-2 infection. However, frequent consumption of polyunsaturated fats is highlighted as a new possible associated factor with COVID-19, which more studies should corroborate as with all novel findings.

ACS Style

Gabriela Macedo-Ojeda; José Francisco Muñoz-Valle; Patricia Yokogawa-Teraoka; Andrea Carolina Machado-Sulbarán; María Guadalupe Loza-Rojas; Atziri Citlally García-Arredondo; Rafael Tejeda-Constantini; Alejandra Natali Vega-Magaña; Guillermo González-Estevez; Mariel García-Chagollán; José Sergio Zepeda-Nuño; Jorge Hernández-Bello. COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality. International Journal of Environmental Research and Public Health 2021, 18, 8995 .

AMA Style

Gabriela Macedo-Ojeda, José Francisco Muñoz-Valle, Patricia Yokogawa-Teraoka, Andrea Carolina Machado-Sulbarán, María Guadalupe Loza-Rojas, Atziri Citlally García-Arredondo, Rafael Tejeda-Constantini, Alejandra Natali Vega-Magaña, Guillermo González-Estevez, Mariel García-Chagollán, José Sergio Zepeda-Nuño, Jorge Hernández-Bello. COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality. International Journal of Environmental Research and Public Health. 2021; 18 (17):8995.

Chicago/Turabian Style

Gabriela Macedo-Ojeda; José Francisco Muñoz-Valle; Patricia Yokogawa-Teraoka; Andrea Carolina Machado-Sulbarán; María Guadalupe Loza-Rojas; Atziri Citlally García-Arredondo; Rafael Tejeda-Constantini; Alejandra Natali Vega-Magaña; Guillermo González-Estevez; Mariel García-Chagollán; José Sergio Zepeda-Nuño; Jorge Hernández-Bello. 2021. "COVID-19 Screening by Anti-SARS-CoV-2 Antibody Seropositivity: Clinical and Epidemiological Characteristics, Comorbidities, and Food Intake Quality." International Journal of Environmental Research and Public Health 18, no. 17: 8995.

Journal article
Published: 05 July 2021 in Vaccines
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The main expected result of a vaccine against viruses is the ability to produce neutralizing antibodies. Currently, several vaccines against SARS-CoV-2 are being applied to prevent mortal complications, being Pfizer-BioNTech (BNT162b2) one of the first to be authorized in the USA and Mexico (11 December 2020). This study evaluated the efficacy of this vaccine on antibody production with neutralizing capacity and its side effects in healthcare workers with and without prior SARS-CoV-2 infection and in a group of unvaccinated individuals with prior COVID-19. The main findings are the production of 100% neutralizing antibodies in both groups after the second dose, well-tolerated adverse effects, the possible presence of immunosenescence, and finally, we support that a single dose of this vaccine in individuals with prior COVID-19 would be sufficient to achieve an immunization comparable to people without prior COVID-19 with a complete vaccination program (2 doses).

ACS Style

José Morales-Núñez; José Muñoz-Valle; Carlos Meza-López; Lin-Fa Wang; Andrea Machado Sulbarán; Paola Torres-Hernández; Martín Bedolla-Barajas; Brenda De la O-Gómez; Paulina Balcázar-Félix; Jorge Hernández-Bello. Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection. Vaccines 2021, 9, 742 .

AMA Style

José Morales-Núñez, José Muñoz-Valle, Carlos Meza-López, Lin-Fa Wang, Andrea Machado Sulbarán, Paola Torres-Hernández, Martín Bedolla-Barajas, Brenda De la O-Gómez, Paulina Balcázar-Félix, Jorge Hernández-Bello. Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection. Vaccines. 2021; 9 (7):742.

Chicago/Turabian Style

José Morales-Núñez; José Muñoz-Valle; Carlos Meza-López; Lin-Fa Wang; Andrea Machado Sulbarán; Paola Torres-Hernández; Martín Bedolla-Barajas; Brenda De la O-Gómez; Paulina Balcázar-Félix; Jorge Hernández-Bello. 2021. "Neutralizing Antibodies Titers and Side Effects in Response to BNT162b2 Vaccine in Healthcare Workers with and without Prior SARS-CoV-2 Infection." Vaccines 9, no. 7: 742.

Journal article
Published: 28 May 2021 in Journal of Clinical Medicine
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Background: The immunomodulatory effects of vitamin D are known to be beneficial in viral infections; it is also known that its deficiency is associated with a prognosis more critical of Coronavirus Disease 2019. This study aimed to determine baseline vitamin D serum concentrations and the effects of its supplementation in asymptomatic or mildly symptomatic Coronavirus Disease 2019 outpatients. Methods: 42 outpatients were included, 22 of which received a supplement of 10,000 IU of vitamin D3 for 14 days; the remaining 20 outpatients were designated as a control group. Serum levels of transferrin, ferritin, vitamin D, and D-dimer were measured at baseline in both groups. After 14 days, serum levels of total vitamin D were determined in the supplemented group. Results: At baseline, only 19% of infected outpatients had vitamin D levels corresponding to sufficiency. All outpatients with vitamin D insufficiency had at least one symptom associated with the disease, while only 75% of patients with symptoms presented sufficiency. On the seventh and fourteenth day of follow-up, the supplemented group presented fewer symptoms with respect to those non-supplemented. A vitamin D3 dose of 10,000 IU/daily for 14 days was sufficient to raise vitamin D serum concentrations. Conclusions: Immunomodulatory effects of vitamin D appear to be linked to the development of symptoms in positive outpatients. Vitamin D supplementation could have significant benefits in the Western Mexican population.

ACS Style

Gabriela Sánchez-Zuno; Guillermo González-Estevez; Mónica Matuz-Flores; Gabriela Macedo-Ojeda; Jorge Hernández-Bello; Jesús Mora-Mora; Edsaúl Pérez-Guerrero; Mariel García-Chagollán; Natali Vega-Magaña; Francisco Turrubiates-Hernández; Andrea Machado-Sulbaran; José Muñoz-Valle. Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation. Journal of Clinical Medicine 2021, 10, 2378 .

AMA Style

Gabriela Sánchez-Zuno, Guillermo González-Estevez, Mónica Matuz-Flores, Gabriela Macedo-Ojeda, Jorge Hernández-Bello, Jesús Mora-Mora, Edsaúl Pérez-Guerrero, Mariel García-Chagollán, Natali Vega-Magaña, Francisco Turrubiates-Hernández, Andrea Machado-Sulbaran, José Muñoz-Valle. Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation. Journal of Clinical Medicine. 2021; 10 (11):2378.

Chicago/Turabian Style

Gabriela Sánchez-Zuno; Guillermo González-Estevez; Mónica Matuz-Flores; Gabriela Macedo-Ojeda; Jorge Hernández-Bello; Jesús Mora-Mora; Edsaúl Pérez-Guerrero; Mariel García-Chagollán; Natali Vega-Magaña; Francisco Turrubiates-Hernández; Andrea Machado-Sulbaran; José Muñoz-Valle. 2021. "Vitamin D Levels in COVID-19 Outpatients from Western Mexico: Clinical Correlation and Effect of Its Supplementation." Journal of Clinical Medicine 10, no. 11: 2378.

Research article
Published: 24 November 2020 in Journal of Clinical Laboratory Analysis
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Background Metabolic syndrome (MetS) prevalence in rheumatoid arthritis (RA) patients is known to vary considerably across the world. This study aimed to determine the prevalence of MetS in RA patients from western Mexico and to analyze the interrelation of the MetS components with the clinical variables of RA. Methods This case‐control study included 216 RA patients and 260 control subjects (CS). MetS prevalence was determined according to the NCEP/ATP III and the Latin American Consensus of the Latin American Diabetes Association (ALAD) criteria. Results MetS was observed in 30.6% RA patients and 33.3% of controls (p > 0.05) according to NCEP/ATP III and 28.7% in RA patients and 31.1% for controls using ALAD criteria. Total cholesterol, LDL‐C, and Castelli's I‐II indexes were lower in RA (p < 0.001) than in CS. The RA patients with MetS had more swollen joints than those without MetS (p = 0.018). In RA patients with MetS, DAS‐28 score correlated with smoking index (rho = 0.4601, p = 0.0004) and VLDL‐C (rho = 0.3108, p = 0.0056); similarly, rheumatoid factor (RF) correlated with age (rho = 0.2031, p = 0.0027), smoking index (rho = 0.3404, p < 0.0001), triglycerides (rho = 0.1958, p = 0.0039), and VLDL‐C (rho = 0.1761, p = 0.0162). Conclusions The MetS prevalence in RA patients from western Mexico is not higher than controls; however, in RA patients with MetS, some inflammatory markers are associated with MetS components; thus, the control of MetS in RA could be beneficial to regulate disease activity.

ACS Style

Mariel García‐Chagollán; Susana Elizabeth Hernández‐Martínez; Alma Elizabeth Rojas‐Romero; José Francisco Muñoz‐Valle; Ramón Sigala‐Arellano; Sergio Cerpa‐Cruz; José Javier Morales‐Núñez; José Alvaro Lomelí‐Nieto; Gabriela Macedo Ojeda; Jorge Hernández‐Bello. Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components. Journal of Clinical Laboratory Analysis 2020, 35, e23666 .

AMA Style

Mariel García‐Chagollán, Susana Elizabeth Hernández‐Martínez, Alma Elizabeth Rojas‐Romero, José Francisco Muñoz‐Valle, Ramón Sigala‐Arellano, Sergio Cerpa‐Cruz, José Javier Morales‐Núñez, José Alvaro Lomelí‐Nieto, Gabriela Macedo Ojeda, Jorge Hernández‐Bello. Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components. Journal of Clinical Laboratory Analysis. 2020; 35 (3):e23666.

Chicago/Turabian Style

Mariel García‐Chagollán; Susana Elizabeth Hernández‐Martínez; Alma Elizabeth Rojas‐Romero; José Francisco Muñoz‐Valle; Ramón Sigala‐Arellano; Sergio Cerpa‐Cruz; José Javier Morales‐Núñez; José Alvaro Lomelí‐Nieto; Gabriela Macedo Ojeda; Jorge Hernández‐Bello. 2020. "Metabolic syndrome in rheumatoid arthritis patients: Relationship among its clinical components." Journal of Clinical Laboratory Analysis 35, no. 3: e23666.

Other
Published: 07 June 2020
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After weeks under lockdown, metropolitan areas fighting the spread of COVID-19 aim to balance public health goals with social and economic standards for well-being. Mathematical models of disease transmission seeking to evaluate mitigation strategies must assess the possible impacts of social distancing, economic lockdowns and other measures. However, obscure relations between model parameters and real-world phenomena complicate such analyses. Here, we use a high-resolution metapopulation model of Guadalajara (GDL, Western Mexico) to represent daily mobility patterns driven by economic activities and their relation to epidemic growth. Given the prominence of essential activities in the city’s economy, we find that strategies aiming to mitigate the risk of out-of-home interactions are insufficient to stop the catastrophic spread of COVID-19. Using baseline reproduction numbers R0 = [2.5, 3.0] in the absence of interventions, our simulations suggest that household transmission alone can make Rt ∼ 1, and is estimated to drive 70 ±15% of current epidemic growth. This sets an upper bound for the impact of mobility-based interventions, which are unlikely to lower Rt below 1.3 and must be complemented with aggressive campaigns for early case detection and isolation. As laboratory testing and health services become insufficient to meet demand in GDL and most other cities, we propose that cities facilitate guidelines and equipment to help people curb spreading within their own homes. Postponing these actions will increase their economic cost and decrease their potential returns.Author summaryPublic health strategies to mitigate the spread of COVID-19 in metropolitan areas have focused on preventing transmission in schools, work sites and other public spaces. Here, we use a demographically- and spatially-explicit model of Guadalajara (GDL, Western Mexico) to represent economic lockdowns and their impact on disease spread. Our findings suggest that viral exposure within households accounts for 70±15% of the epidemic’s current growth rate. This highlights the importance of early case detection and isolation as necessary measures to prevent the spread of COVID-19 between strangers and close contacts alike.

ACS Style

Noel Gutiérrez Brizuela; Humberto Gutiérrez Pulido; Kimberlyn Roosa; Néstor García Chan; Jorge Hernández-Bello; José Francisco Muñoz-Valle; Gabriela Macedo-Ojeda; Guillermo González-Estevez; Javier Alonso López-Chávez; Ricardo Villanueva-Lomelí; Gerardo Chowell Puente. Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities. 2020, 1 .

AMA Style

Noel Gutiérrez Brizuela, Humberto Gutiérrez Pulido, Kimberlyn Roosa, Néstor García Chan, Jorge Hernández-Bello, José Francisco Muñoz-Valle, Gabriela Macedo-Ojeda, Guillermo González-Estevez, Javier Alonso López-Chávez, Ricardo Villanueva-Lomelí, Gerardo Chowell Puente. Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities. . 2020; ():1.

Chicago/Turabian Style

Noel Gutiérrez Brizuela; Humberto Gutiérrez Pulido; Kimberlyn Roosa; Néstor García Chan; Jorge Hernández-Bello; José Francisco Muñoz-Valle; Gabriela Macedo-Ojeda; Guillermo González-Estevez; Javier Alonso López-Chávez; Ricardo Villanueva-Lomelí; Gerardo Chowell Puente. 2020. "Prevention of household transmission crucial to stop the catastrophic spread of COVID-19 in cities." , no. : 1.

Original article
Published: 07 November 2019 in Molecular Genetics & Genomic Medicine
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Background Macrophage migration inhibitory factor (MIF) is a cytokine capable of stimulating inflammatory cytokine and matrix metalloproteinase production from macrophages and synovial fibroblasts, which leads to persistent inflammation and bone degradation, two of the major pathological processes in rheumatoid arthritis (RA). The aim of this study was to evaluate the association of MIF promoter polymorphisms (−794CATT5‐8rs5844572 and −173G > C, rs755622), circulating MIF levels, and mRNA expression with RA susceptibility and disease activity. Methods A case–control study was conducted in 200 RA patients and 200 control subjects (CS) from Southern Mexico. Genotyping was performed by conventional PCR and PCR‐RFLP methods. MIF mRNA expression was quantified by real‐time PCR and MIF serum levels were determined by an ELISA kit. Results The 7,7 (−794CATT5‐8) and −173CC (−173G > C) genotypes were associated with higher disease activity in RA patients. MIF serum levels were increased, and MIF mRNA expression was reduced in RA patients as compared to CS. In addition, RA patients with moderate disease activity had higher MIF levels than those with low disease activity. The −794CATT5‐8 and −173G > C MIF polymorphisms were not associated with RA susceptibility. Conclusion These results suggest an important role of MIF polymorphisms and MIF serum levels with disease activity in RA.

ACS Style

Guillermo Santoscoy‐Ascencio; Christian Johana Baños‐Hernández; José Eduardo Navarro‐Zarza; Jorge Hernández‐Bello; Richard Bucala; Andres López‐Quintero; Emmanuel Valdés‐Alvarado; Isela Parra‐Rojas; Berenice Illades‐Aguiar; José Francisco Muñoz‐Valle. Macrophage migration inhibitory factor promoter polymorphisms are associated with disease activity in rheumatoid arthritis patients from Southern Mexico. Molecular Genetics & Genomic Medicine 2019, 8, e1037 .

AMA Style

Guillermo Santoscoy‐Ascencio, Christian Johana Baños‐Hernández, José Eduardo Navarro‐Zarza, Jorge Hernández‐Bello, Richard Bucala, Andres López‐Quintero, Emmanuel Valdés‐Alvarado, Isela Parra‐Rojas, Berenice Illades‐Aguiar, José Francisco Muñoz‐Valle. Macrophage migration inhibitory factor promoter polymorphisms are associated with disease activity in rheumatoid arthritis patients from Southern Mexico. Molecular Genetics & Genomic Medicine. 2019; 8 (1):e1037.

Chicago/Turabian Style

Guillermo Santoscoy‐Ascencio; Christian Johana Baños‐Hernández; José Eduardo Navarro‐Zarza; Jorge Hernández‐Bello; Richard Bucala; Andres López‐Quintero; Emmanuel Valdés‐Alvarado; Isela Parra‐Rojas; Berenice Illades‐Aguiar; José Francisco Muñoz‐Valle. 2019. "Macrophage migration inhibitory factor promoter polymorphisms are associated with disease activity in rheumatoid arthritis patients from Southern Mexico." Molecular Genetics & Genomic Medicine 8, no. 1: e1037.

Primary research
Published: 02 September 2019 in Cancer Cell International
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Cervical cancer (CC) is the second most common cancer in less developed countries and the second leading cause of death by cancer in women worldwide. The 99% of CC patients are infected with the Human Papilloma Virus (HPV), being HPV16 and HPV18 infection the most frequent. Even though HPV is considered to be a necessary factor for the development of CC, it is not enough, as it requires the participation of other factors such as the hormonal ones. Several studies have demonstrated the requirement of estrogen and its receptors (ERα, ERβ, and GPER) in the precursor lesions progress towards CC. Also, prolactin (PRL) and its receptor (PRLR) have been associated with CC. The molecular mechanisms underlying the cooperation of these hormones with the viral oncoproteins are not well elucidated. For this reason, this study focused on analyzing the contribution of 17β-estradiol (E2), PRL, and HPV on the expression and localization of hormone receptors, as well as to evaluate whether these hormones may promote greater expression of HPV oncogenes and contribute to tumor progression. qPCR was used to evaluate the effect of E2 and PRL on the expression of E6 and E7 oncoproteins in HeLa and SiHa cervical cancer cells lines. HaCaT cells were transduced with the viral oncogenes E6 and E7 from HPV 16 and 18. ERα, ERβ, GPER, and PRLR expression and localization were evaluated by qPCR, Western blot and immunofluorescence. E2 and PRL induce E6/E7 oncogenes expression in HeLa and SiHa cells. E6 and E7 oncogenes of HPV16/18 significantly increased the protein expression of ERα, GPER, and PRLR. ERβ was positively regulated only by E6 oncogenes of HPV16/18. Besides, some of these oncogenes modify the location of PRLR toward cytoplasm, and ERα, ERβ, and GPER mainly to the nucleus. Our studies suggest that the mutual regulation between E2, PRL, and HPV oncogenes could cooperate with the carcinogenesis process in CC.

ACS Style

Inocencia Guadalupe Ramírez-López; Adrián Ramírez De Arellano; Luis Felipe Jave-Suarez; Christian David Hernández-Silva; Mariel García-Chagollan; Jorge Hernández-Bello; Edgar I. Lopez-Pulido; José Macias-Barragan; Margarita Montoya-Buelna; José Francisco Muñoz-Valle; Ana Laura Pereira-Suárez. Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors. Cancer Cell International 2019, 19, 1 -10.

AMA Style

Inocencia Guadalupe Ramírez-López, Adrián Ramírez De Arellano, Luis Felipe Jave-Suarez, Christian David Hernández-Silva, Mariel García-Chagollan, Jorge Hernández-Bello, Edgar I. Lopez-Pulido, José Macias-Barragan, Margarita Montoya-Buelna, José Francisco Muñoz-Valle, Ana Laura Pereira-Suárez. Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors. Cancer Cell International. 2019; 19 (1):1-10.

Chicago/Turabian Style

Inocencia Guadalupe Ramírez-López; Adrián Ramírez De Arellano; Luis Felipe Jave-Suarez; Christian David Hernández-Silva; Mariel García-Chagollan; Jorge Hernández-Bello; Edgar I. Lopez-Pulido; José Macias-Barragan; Margarita Montoya-Buelna; José Francisco Muñoz-Valle; Ana Laura Pereira-Suárez. 2019. "Interaction between 17β-estradiol, prolactin and human papillomavirus induce E6/E7 transcript and modulate the expression and localization of hormonal receptors." Cancer Cell International 19, no. 1: 1-10.

Original article
Published: 29 July 2019 in Clinical and Experimental Medicine
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Systemic sclerosis (SSc) is a rare autoimmune disease with high mortality, characterized by chronic inflammation and fibrosis, which are processes associated with higher serum tumor necrosis factor-α (sTNF-α) levels. TNFA -308G>A and -238G>A polymorphisms have been associated with higher sTNF-α levels. In this study, we genotyped the TNFA -308G>A and -238G>A polymorphisms in 53 SSc patients and 115 unrelated control subjects (CS) from southern Mexico. The TNFA mRNA expression and sTNF-α levels were also quantified by qPCR and enzyme-linked immunosorbent assays, respectively. TNFA -308GA genotype was associated with disease susceptibility according to a codominant genetic model (OR = 3.2, 95% CI 1.05–9.75, p = 0.03), and with higher anti-fibrillarin antibodies (p = 0.01), and higher skin thickening (p = 0.006). TNFA -238GA was not associated with SSc risk. TNFA mRNA expression and sTNF-α levels were similar between SSc patients and CS and were not statistically associated with the TNFA polymorphisms; however, a correlation (rho = 0.362, p = 0.009) between sTNF-α levels with anti-RNA polymerase III antibodies was observed in the SSc patients. In conclusion, the -308G>A polymorphism is a genetic marker of SSc susceptibility in population from southern Mexico, and it is associated with skin thickening and anti-fibrillarin antibodies. In addition, sTNF-α levels correlate positively with the anti-RNA pol III antibodies levels.

ACS Style

José Alvaro Lomelí-Nieto; José Francisco Muñoz-Valle; Christian Johana Baños-Hernández; José Eduardo Navarro-Zarza; María Guadalupe Ramírez-Dueñas; Pedro Ernesto Sánchez-Hernández; Andrea Carolina Machado-Sulbaran; Isela Parra-Rojas; Mariel García-Chagollán; Jorge Hernández-Bello. TNFA -308G>A and -238G>A polymorphisms and risk to systemic sclerosis: impact on TNF-α serum levels, TNFA mRNA expression, and autoantibodies. Clinical and Experimental Medicine 2019, 19, 439 -447.

AMA Style

José Alvaro Lomelí-Nieto, José Francisco Muñoz-Valle, Christian Johana Baños-Hernández, José Eduardo Navarro-Zarza, María Guadalupe Ramírez-Dueñas, Pedro Ernesto Sánchez-Hernández, Andrea Carolina Machado-Sulbaran, Isela Parra-Rojas, Mariel García-Chagollán, Jorge Hernández-Bello. TNFA -308G>A and -238G>A polymorphisms and risk to systemic sclerosis: impact on TNF-α serum levels, TNFA mRNA expression, and autoantibodies. Clinical and Experimental Medicine. 2019; 19 (4):439-447.

Chicago/Turabian Style

José Alvaro Lomelí-Nieto; José Francisco Muñoz-Valle; Christian Johana Baños-Hernández; José Eduardo Navarro-Zarza; María Guadalupe Ramírez-Dueñas; Pedro Ernesto Sánchez-Hernández; Andrea Carolina Machado-Sulbaran; Isela Parra-Rojas; Mariel García-Chagollán; Jorge Hernández-Bello. 2019. "TNFA -308G>A and -238G>A polymorphisms and risk to systemic sclerosis: impact on TNF-α serum levels, TNFA mRNA expression, and autoantibodies." Clinical and Experimental Medicine 19, no. 4: 439-447.

Original article
Published: 25 March 2019 in Clinical Rheumatology
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Prolactin (PRL) is a sex hormone with immunomodulatory properties, and it is associated with the clinical activity of rheumatoid arthritis (RA). The -1149G>T polymorphism at the prolactin (PRL) gene has been associated with autoimmune diseases, but its functional effect is unclear. To analyze the association of the PRL -1149G>T polymorphism with disease susceptibility, mRNA, and protein expression of PRL in RA patients from Southern Mexico. We included 300 RA patients and 300 control subjects (CS). Genotypes were identified by the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, the PRL mRNA expression was determined by real-time PCR, and PRL serum levels were measured by enzyme-linked immunosorbent assay. Applying genetic models of inheritance (dominant, recessive, and additive), we found an association between the T allele and decreased RA susceptibility (OR = 0.55, 95% CI 0.35-0.87, p = 0.009; OR = 0.09, 95% CI 0.012-0.76, p = 0.011; OR = 0.49, 95% CI 0.32-0.76, p = 0.001, respectively). RA patients had higher mRNA expression and soluble levels of PRL than CS (p < 0.05). The PRL serum levels were similar in RA and CS according to genotypes. However, in CS, carriers of GT and TT genotypes showed lower PRL mRNA expression than GG genotype carriers (7.1-fold and 20-fold respectively, p = 0.006). This study demonstrated that the PRL -1149T allele is a genetic marker of decrease risk to RA in population from Southern Mexico, and it is associated with low PRL mRNA. • PRL -1149T allele is a marker of decreased RA susceptibility in population from southern Mexico. • PRL -1149TT genotype is associated with low PRL mRNA expression. • RA patients have higher mRNA expression and soluble levels of PRL than healthy subjects. • PRL serum levels are higher in those RA patients with < 2 years of disease evolution.

ACS Style

J. E. Navarro-Zarza; J. F. Muñoz-Valle; C. J. Baños-Hernández; I. Parra-Rojas; Z. Reyes-Castillo; H. Rangel-Villalobos; J. Hernández-Bello. PRL -1149T allele (rs1341239) is associated with decreased risk of rheumatoid arthritis in population from southern Mexico: analysis of mRNA expression and PRL serum levels. Clinical Rheumatology 2019, 38, 2089 -2097.

AMA Style

J. E. Navarro-Zarza, J. F. Muñoz-Valle, C. J. Baños-Hernández, I. Parra-Rojas, Z. Reyes-Castillo, H. Rangel-Villalobos, J. Hernández-Bello. PRL -1149T allele (rs1341239) is associated with decreased risk of rheumatoid arthritis in population from southern Mexico: analysis of mRNA expression and PRL serum levels. Clinical Rheumatology. 2019; 38 (8):2089-2097.

Chicago/Turabian Style

J. E. Navarro-Zarza; J. F. Muñoz-Valle; C. J. Baños-Hernández; I. Parra-Rojas; Z. Reyes-Castillo; H. Rangel-Villalobos; J. Hernández-Bello. 2019. "PRL -1149T allele (rs1341239) is associated with decreased risk of rheumatoid arthritis in population from southern Mexico: analysis of mRNA expression and PRL serum levels." Clinical Rheumatology 38, no. 8: 2089-2097.

Research article
Published: 06 November 2018 in Journal of Clinical Laboratory Analysis
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Background CD40 is a costimulatory molecule for B cells, and CD154 is a marker of CD4+ T cells activation. CD40‐CD154 interaction promotes pro‐inflammatory cytokines secretion and autoantibodies production. PTPN22 gene encodes LYP protein, an inhibitor of T‐ and B‐cell activation. PTPN22 1858C>T polymorphism confers rheumatoid arthritis (RA) susceptibility. Hence, we evaluate the relationship between 1858C>T polymorphism with CD40 and CD154 expression and IFN‐γ secretion in RA patients. Methods PTPN22 1858C>T polymorphism was genotyped in 315 RA patients and 315 control subjects (CS) using PCR‐RFLP method. Later, we selected only ten anti‐CCP‐positive RA patients, naïve to disease‐modifying antirheumatic drugs and ten CS, all with known 1858C>T PTPN22 genotype. The CD40 and CD154 membrane expressions were determined by flow cytometry in peripheral B and T cells, correspondingly. Results The B cells percentage and mCD40 expression were similar between RA and CS (P > 0.05) and we did not find an association between these variables and the 1858C>T polymorphism. The CD4+ T cells percentage was higher in RA patients than CS (P = 0.003), and in the RA group, the CD4+ T cells percentage and mCD154 expression were higher in the 1858 T allele carriers (P = 0.008 and P = 0.032, respectively). The IFN‐γ levels were lower in RA patients carrying the PTPN22 risk allele (P = 0.032). Conclusion The PTPN22 1858 T risk allele is associated with increased CD4+ T cells percentage and high mCD154 expression in RA patients, which could favor the pro‐inflammatory cytokine release and the establishment of the inflammatory response at the seropositive RA.

ACS Style

Yeniley Ruiz-Noa; Jorge Hernández-Bello; Mara A. Llamas-Covarrubias; Claudia A. Palafox-Sánchez; Edith Oregon-Romero; Pedro Ernesto Sánchez-Hernández; Maria Guadalupe Ramírez-Dueñas; Isela Parra-Rojas; Jose Francisco Muñoz-Valle. PTPN22 1858C>T polymorphism is associated with increased CD154 expression and higher CD4+ T cells percentage in rheumatoid arthritis patients. Journal of Clinical Laboratory Analysis 2018, 33, e22710 .

AMA Style

Yeniley Ruiz-Noa, Jorge Hernández-Bello, Mara A. Llamas-Covarrubias, Claudia A. Palafox-Sánchez, Edith Oregon-Romero, Pedro Ernesto Sánchez-Hernández, Maria Guadalupe Ramírez-Dueñas, Isela Parra-Rojas, Jose Francisco Muñoz-Valle. PTPN22 1858C>T polymorphism is associated with increased CD154 expression and higher CD4+ T cells percentage in rheumatoid arthritis patients. Journal of Clinical Laboratory Analysis. 2018; 33 (3):e22710.

Chicago/Turabian Style

Yeniley Ruiz-Noa; Jorge Hernández-Bello; Mara A. Llamas-Covarrubias; Claudia A. Palafox-Sánchez; Edith Oregon-Romero; Pedro Ernesto Sánchez-Hernández; Maria Guadalupe Ramírez-Dueñas; Isela Parra-Rojas; Jose Francisco Muñoz-Valle. 2018. "PTPN22 1858C>T polymorphism is associated with increased CD154 expression and higher CD4+ T cells percentage in rheumatoid arthritis patients." Journal of Clinical Laboratory Analysis 33, no. 3: e22710.

Journal article
Published: 01 January 2018 in Archives of Medical Science
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Introduction : Prolactin (PRL) is a 23-kDa protein that can be synthesized and secreted by pituitary and extrapituitary tissues such as immune cells due to its expression being regulated by two independent promoter regions. The promoter which is responsible for extrapituitary expression contains...

ACS Style

Jorge Hernández-Bello; Claudia A. Palafox-Sanchez; Samuel García-Arellano; Zyanya Reyes-Castillo; Ana L. Pereira-Suárez; Isela Parra-Rojas; José E. Navarro-Zarza; Ulises De La Cruz-Mosso; Nora M. Torres-Carrillo; José Francisco Muñoz-Valle. Association of extrapituitary prolactin promoter polymorphism with disease susceptibility and anti-RNP antibodies in Mexican patients with systemic lupus erythematosus. Archives of Medical Science 2018, 14, 1025 -1032.

AMA Style

Jorge Hernández-Bello, Claudia A. Palafox-Sanchez, Samuel García-Arellano, Zyanya Reyes-Castillo, Ana L. Pereira-Suárez, Isela Parra-Rojas, José E. Navarro-Zarza, Ulises De La Cruz-Mosso, Nora M. Torres-Carrillo, José Francisco Muñoz-Valle. Association of extrapituitary prolactin promoter polymorphism with disease susceptibility and anti-RNP antibodies in Mexican patients with systemic lupus erythematosus. Archives of Medical Science. 2018; 14 (5):1025-1032.

Chicago/Turabian Style

Jorge Hernández-Bello; Claudia A. Palafox-Sanchez; Samuel García-Arellano; Zyanya Reyes-Castillo; Ana L. Pereira-Suárez; Isela Parra-Rojas; José E. Navarro-Zarza; Ulises De La Cruz-Mosso; Nora M. Torres-Carrillo; José Francisco Muñoz-Valle. 2018. "Association of extrapituitary prolactin promoter polymorphism with disease susceptibility and anti-RNP antibodies in Mexican patients with systemic lupus erythematosus." Archives of Medical Science 14, no. 5: 1025-1032.

Original article
Published: 17 November 2017 in Autoimmunity
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Rheumatoid arthritis (RA) is an autoimmune disease characterized by inflammation and pro-inflammatory cytokines production. IL-1Ra is an anti-inflammatory cytokine codified by IL1RN gene that blocks IL-1 signalling. A VNTR polymorphism of 86 bp in IL1RN gene has been associated with RA risk and regulation of IL-1Ra expression. In this study, we determined mRNA and protein expression of IL-1Ra in RA patients and control subjects (CS). This study included 85 RA patients classified according to the ACR/EULAR 2010 criteria and 67 CS. Polymerase chain reaction was used to identify IL1RN VNTR polymorphism, the expression of sIL-1Ra (secreted isoform) mRNA was determined by SYBR Green-based real time quantitave-PCR assay, and IL-1Ra soluble levels quantification was evaluated by ELISA test. RA patients had higher soluble levels of IL-1Ra than CS (p < .01), sIL-1Ra mRNA expression was higher in RA patients compared to CS (p < .01). Carriers of IL1RN*2/2 homozygous genotype show increased IL-1Ra soluble levels compared to IL1RN*long/long and IL1RN*2/long genotypes (p < .05) in the CS group, whereas mRNA expression in carriers of IL1RN*2/2 genotype was 1.2 times higher compared to IL1RN*long/long genotypes in the same group. Regarding RA patients, high expression of sIL-1Ra mRNA on carriers of IL1RN*long/long genotype was observed. Nevertheless, in RA patients IL-1Ra soluble levels among genotypes did not show significant differences. High expression of IL-1Ra in RA patients under treatment or not with antirheumatic drugs was detected. Additionally, carriers of IL1RN*2/2 genotype had higher IL-1Ra expression than carriers of other genotypes.

ACS Style

S. Ramírez-Pérez; U. De La Cruz-Mosso; Jorge Hernández-Bello; G. E. Martínez-Bonilla; M. G. Ramírez-Dueñas; A. L. Pereira-Suárez; I. Parra Rojas; E. Martínez-López; J. Macías-Barragán; J. F. Muñoz-Valle. High expression of interleukine-1 receptor antagonist in rheumatoid arthritis: Association with IL1RN*2/2 genotype. Autoimmunity 2017, 50, 468 -475.

AMA Style

S. Ramírez-Pérez, U. De La Cruz-Mosso, Jorge Hernández-Bello, G. E. Martínez-Bonilla, M. G. Ramírez-Dueñas, A. L. Pereira-Suárez, I. Parra Rojas, E. Martínez-López, J. Macías-Barragán, J. F. Muñoz-Valle. High expression of interleukine-1 receptor antagonist in rheumatoid arthritis: Association with IL1RN*2/2 genotype. Autoimmunity. 2017; 50 (8):468-475.

Chicago/Turabian Style

S. Ramírez-Pérez; U. De La Cruz-Mosso; Jorge Hernández-Bello; G. E. Martínez-Bonilla; M. G. Ramírez-Dueñas; A. L. Pereira-Suárez; I. Parra Rojas; E. Martínez-López; J. Macías-Barragán; J. F. Muñoz-Valle. 2017. "High expression of interleukine-1 receptor antagonist in rheumatoid arthritis: Association with IL1RN*2/2 genotype." Autoimmunity 50, no. 8: 468-475.

Original article
Published: 30 September 2017 in Clinical Rheumatology
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The objective of this study was to determine the association of the CD40LG 3′-UTR (CA)n microsatellite with rheumatoid arthritis (RA) and CD40LG mRNA levels in females from western Mexico. A case-control study with 219 RA patients and 175 control subjects (CS) was conducted. Genotyping was performed by polymerase chain reaction (PCR), X2 test was used to compare genotype and allele frequencies, and odds ratios and 95% confidence intervals were calculated to evaluate the association between RA and the microsatellite. CD40LG mRNA expression was assessed by real-time quantitative PCR. For comparisons between groups, Kruskal-Wallis or Mann-Whitney U tests for non-parametric data and ANOVA test for parametric data were performed. Among the 13 different alleles identified, CA25 was the most represented (45.4% RA and 46.3% CS). Stratification according to CA repeats as CA25 showed a tendency towards a higher frequency of >CA25 alleles in RA patients (29%) compared to CS (23.4%). There was no association between any genotype and the clinical parameters of RA patients. According to the 2-∆∆Cq method, CD40LG mRNA expression in RA patients was 4.5-fold higher compared to CS; this difference was significant when assessed by the 2-∆Cq method (p = 0.028). Compared to carriers of the CA25/CA25 genotype, CS carrying the CA25/>CA25 carriers. The 3′-UTR CD40LG (CA)n microsatellite is not a genetic marker for RA in western Mexican population; however, results suggest that it plays a role in the CD40LG mRNA expression.

ACS Style

I. V. Román-Fernández; G. A. Sánchez-Zuno; J. R. Padilla-Gutiérrez; S. Cerpa-Cruz; J. Hernández-Bello; Y. Valle; M. G. Ramírez-Dueñas; C. Carrillo; J. F. Muñoz-Valle. The 3?-UTR (CA)n microsatellite on CD40LG gene as a possible genetic marker for rheumatoid arthritis in Mexican population: impact on CD40LG mRNA expression. Clinical Rheumatology 2017, 37, 345 -353.

AMA Style

I. V. Román-Fernández, G. A. Sánchez-Zuno, J. R. Padilla-Gutiérrez, S. Cerpa-Cruz, J. Hernández-Bello, Y. Valle, M. G. Ramírez-Dueñas, C. Carrillo, J. F. Muñoz-Valle. The 3?-UTR (CA)n microsatellite on CD40LG gene as a possible genetic marker for rheumatoid arthritis in Mexican population: impact on CD40LG mRNA expression. Clinical Rheumatology. 2017; 37 (2):345-353.

Chicago/Turabian Style

I. V. Román-Fernández; G. A. Sánchez-Zuno; J. R. Padilla-Gutiérrez; S. Cerpa-Cruz; J. Hernández-Bello; Y. Valle; M. G. Ramírez-Dueñas; C. Carrillo; J. F. Muñoz-Valle. 2017. "The 3?-UTR (CA)n microsatellite on CD40LG gene as a possible genetic marker for rheumatoid arthritis in Mexican population: impact on CD40LG mRNA expression." Clinical Rheumatology 37, no. 2: 345-353.

Original article
Published: 30 September 2017 in Clinical and Experimental Medicine
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Psoriatic arthritis (PsA) is an autoimmune inflammatory disease associated with psoriasis. The cause of this pathology is still unknown, but research suggests the diseases are caused by a deregulated cytokine production. MIF is a cytokine associated with immunomodulation of Th1, Th2, and Th17 cytokine profiles in inflammatory diseases. Based on this knowledge, the aim of this study was to determine the association of MIF and TNFA expression with Th1, Th2, and Th17 cytokine profiles in serum levels of PsA patients. A cross-sectional study was performed in 50 PsA patients and 30 control subjects (CS). The cytokine profiles were quantified by BioPlex MagPix system and the mRNA expression levels by real-time PCR. TNFA mRNA expression was 138.81-folds higher in PsA patients than CS (p < 0.001). Regarding MIF mRNA expression, no significant differences were observed; however, a positive correlation was identified between MIF mRNA expression and PsA time of evolution (r = − 0.53, p = 0.009). An increase of Th1 (IFNγ: PsA = 37.1 pg/mL vs. CS = 17 pg/mL, p < 0.05; TNFα: PsA = 24.6 pg/mL vs. CS = 9.8 pg/mL, p < 0.0001) and Th17 cytokine profiles (IL-17: PsA = 6.4 pg/mL vs. CS = 2.7 pg/mL, p < 0.05; IL-22: PsA = 8.4 pg/mL vs. CS = 1.8 pg/mL, p < 0.001), were found in PsA patients. Th2 cytokines were not significantly different in both groups. In conclusion, a high expression of TNFA mRNA, as well as an increase of Th1 and Th17 cytokine profiles evaluated by IFNγ, TNFα, IL-17, and IL-22 cytokines, was observed in PsA patients.

ACS Style

L. A. Bautista-Herrera; Ulises De La Cruz-Mosso; R. Morales-Zambrano; Delfina Guadalupe Villanueva; J. Hernández-Bello; M. G. Ramírez-Dueñas; E. Martínez-López; L. M. Brennan-Bourdon; C. J. Baños-Hernández; J. F. Muñoz-Valle. Expression of MIF and TNFA in psoriatic arthritis: relationship with Th1/Th2/Th17 cytokine profiles and clinical variables. Clinical and Experimental Medicine 2017, 18, 229 -235.

AMA Style

L. A. Bautista-Herrera, Ulises De La Cruz-Mosso, R. Morales-Zambrano, Delfina Guadalupe Villanueva, J. Hernández-Bello, M. G. Ramírez-Dueñas, E. Martínez-López, L. M. Brennan-Bourdon, C. J. Baños-Hernández, J. F. Muñoz-Valle. Expression of MIF and TNFA in psoriatic arthritis: relationship with Th1/Th2/Th17 cytokine profiles and clinical variables. Clinical and Experimental Medicine. 2017; 18 (2):229-235.

Chicago/Turabian Style

L. A. Bautista-Herrera; Ulises De La Cruz-Mosso; R. Morales-Zambrano; Delfina Guadalupe Villanueva; J. Hernández-Bello; M. G. Ramírez-Dueñas; E. Martínez-López; L. M. Brennan-Bourdon; C. J. Baños-Hernández; J. F. Muñoz-Valle. 2017. "Expression of MIF and TNFA in psoriatic arthritis: relationship with Th1/Th2/Th17 cytokine profiles and clinical variables." Clinical and Experimental Medicine 18, no. 2: 229-235.

Journal article
Published: 01 August 2017 in Medicina Clínica
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The protein tyrosine phosphatase non-receptor type 22 (PTPN22) gene encodes an important negative regulator of T-cell activation, lymphoid-specific phosphatase -Lyp- and has been associated with different autoimmune disorders. The PTPN22 -1123G>C polymorphism appears to affect the transcriptional control of this gene, but to date, the biological significance of this polymorphisms on rheumatoid arthritis (RA) risk remains unknown. We evaluate the association of PTPN22 -1123G>C polymorphism with anti-cyclic citrullinated protein antibodies (anti-CCP) and risk for RA in population from Western Mexico. A transversal analytic study, which enrolled 300 RA patients classified according to ACR-EULAR criteria and 300 control subjects (CS) was conducted. The -1123 G>C polymorphism was genotyped by PCR-RFLP. The anti-CCP antibodies levels were quantified by ELISA kit. We found a higher prevalence of homozygous PTPN22 -1123CC genotype in CS than in RA patients (OR 0.41; 95% confidence interval 0.24-0.71; P=.001), suggesting a potential protective effect against RA. Concerning anti-CCP levels, the CC genotype carriers showed the lowest median levels in RA (P<.05). The PTPN22 -1123CC genotype is a protector factor to RA in a Mexican-mestizo population and is associated with low anti-CCP antibodies.

ACS Style

José Francisco Muñoz-Valle; Jorge Ramón Padilla-Gutiérrez; Jorge Hernández-Bello; Yeniley Ruiz-Noa; Yeminia Valle; Claudia Azucena Palafox-Sánchez; Isela Parra-Rojas; Sergio Ramón Gutiérrez-Ureña; Hector Rangel-Villalobos. Polimorfismo −1123G>C en el gen PTPN22 y anticuerpos antipéptido citrulinado cíclico en la artritis reumatoide. Medicina Clínica 2017, 149, 95 -100.

AMA Style

José Francisco Muñoz-Valle, Jorge Ramón Padilla-Gutiérrez, Jorge Hernández-Bello, Yeniley Ruiz-Noa, Yeminia Valle, Claudia Azucena Palafox-Sánchez, Isela Parra-Rojas, Sergio Ramón Gutiérrez-Ureña, Hector Rangel-Villalobos. Polimorfismo −1123G>C en el gen PTPN22 y anticuerpos antipéptido citrulinado cíclico en la artritis reumatoide. Medicina Clínica. 2017; 149 (3):95-100.

Chicago/Turabian Style

José Francisco Muñoz-Valle; Jorge Ramón Padilla-Gutiérrez; Jorge Hernández-Bello; Yeniley Ruiz-Noa; Yeminia Valle; Claudia Azucena Palafox-Sánchez; Isela Parra-Rojas; Sergio Ramón Gutiérrez-Ureña; Hector Rangel-Villalobos. 2017. "Polimorfismo −1123G>C en el gen PTPN22 y anticuerpos antipéptido citrulinado cíclico en la artritis reumatoide." Medicina Clínica 149, no. 3: 95-100.

Journal article
Published: 01 August 2017 in Gene
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L-selectin gene (SELL) is a candidate gene for the development of acute coronary syndrome (ACS) that contributes to endothelial dysfunction. The -642C>T (rs2205849) and 725C>T (rs2229569) polymorphisms have been associated with changes in gene expression, ligand affinity and increased risk of cardiovascular disease. The aim of this study was to investigate the association between the haplotypes constructed with the -642C>T and 725C>T polymorphisms of the SELL gene, the expression levels of its mRNA and the serum levels of soluble L-selectin with ACS.

ACS Style

Maria Elena Sandoval Pinto; Jorge Ramón Padilla-Gutiérrez; Jorge Hernández-Bello; Diana Emilia Martínez-Fernández; Emmanuel Valdés-Alvarado; José Francisco Muñoz-Valle; H.E. Flores-Salinas; Yeminia Valle. Influence of haplotypes, gene expression and soluble levels of L-selectin on the risk of acute coronary syndrome. Gene 2017, 625, 31 -41.

AMA Style

Maria Elena Sandoval Pinto, Jorge Ramón Padilla-Gutiérrez, Jorge Hernández-Bello, Diana Emilia Martínez-Fernández, Emmanuel Valdés-Alvarado, José Francisco Muñoz-Valle, H.E. Flores-Salinas, Yeminia Valle. Influence of haplotypes, gene expression and soluble levels of L-selectin on the risk of acute coronary syndrome. Gene. 2017; 625 ():31-41.

Chicago/Turabian Style

Maria Elena Sandoval Pinto; Jorge Ramón Padilla-Gutiérrez; Jorge Hernández-Bello; Diana Emilia Martínez-Fernández; Emmanuel Valdés-Alvarado; José Francisco Muñoz-Valle; H.E. Flores-Salinas; Yeminia Valle. 2017. "Influence of haplotypes, gene expression and soluble levels of L-selectin on the risk of acute coronary syndrome." Gene 625, no. : 31-41.

Journal article
Published: 01 July 2017 in Cytokine
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Interleukin 10 (IL-10) is an immunomodulatory cytokinethat plays a central rolein the pathogenesis of autoimmune diseases. Different studies consistently show increased IL-10 serum levels in rheumatoid arthritis (RA) and it appears to be caused by genetic variants. Three polymorphisms situated at positions -1082, -819 and -592 of IL10 gene and its major haplotypes have been associated with regulating IL10 promoter activity. In this study, we evaluated whether IL10 haplotypes are associated with mRNA expression and IL-10 serum levels as well as susceptibility to RA in a Western Mexican population. A total of 240 RA patients and 240 control subjects (CS) were included. Genotyping of IL10 polymorphisms was performed by PCR and PCR-RFLP, respectively. IL10 mRNA expression was determined by real-time PCR and IL-10 serum levels were measured using an ELISA kit. IL10 mRNA expression was 50-fold higher in RA patients than CS (p<0.001), while IL-10 serum levels did not show differences between groups. However, high IL-10 serum levels were positively related to a higherseropositivityfor rheumatoid factor (FR) and anti-CCP antibodies (p<0.05). No significant differences between the distribution of haplotype frequencies were observed between both study groups, but GCC haplotype was associated with higher IL-10 serum levels compared with the ACC and ATA haplotypes in RA patients (p<0.05). In addition, patients carrying ATA and GCC haplotypes showed higher mRNA expression than ACC (5.4-fold and 8.8-fold, respectively) and surprisingly, this trend was reversed in the controls, although it was not significant. In conclusion, our findings suggest that IL10 (GCC, ACC, and ATA) haplotypes may not be a susceptibility marker for RA in a population from Western Mexico. Nevertheless, independently of the presence of these variants, there is an aberrant overexpression of IL10 gene in RA, and it may play an important role in the pathogenesis of RA.

ACS Style

J. Hernández-Bello; E. Oregón-Romero; M. Vázquez-Villamar; S. García-Arellano; Y. Valle; J.R. Padilla-Gutiérrez; I.V. Román-Fernández; C.A. Palafox-Sánchez; G.E. Martínez-Bonilla; J.F. Muñoz-Valle. Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies. Cytokine 2017, 95, 88 -96.

AMA Style

J. Hernández-Bello, E. Oregón-Romero, M. Vázquez-Villamar, S. García-Arellano, Y. Valle, J.R. Padilla-Gutiérrez, I.V. Román-Fernández, C.A. Palafox-Sánchez, G.E. Martínez-Bonilla, J.F. Muñoz-Valle. Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies. Cytokine. 2017; 95 ():88-96.

Chicago/Turabian Style

J. Hernández-Bello; E. Oregón-Romero; M. Vázquez-Villamar; S. García-Arellano; Y. Valle; J.R. Padilla-Gutiérrez; I.V. Román-Fernández; C.A. Palafox-Sánchez; G.E. Martínez-Bonilla; J.F. Muñoz-Valle. 2017. "Aberrant expression of interleukin-10 in rheumatoid arthritis: Relationship with IL10 haplotypes and autoantibodies." Cytokine 95, no. : 88-96.

Original article
Published: 25 March 2017 in Clinical Rheumatology
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Rheumatoid arthritis (RA) is a chronic, systemic disease of unknown etiology. Several studies have reported a variable number of tandem repeat (VNTR) 86 bp (rs2234663) in the intron 2 of IL1RN gene with RA risk. The present study was designed to determine the frequencies of this polymorphism in patients with RA and control subjects (CS) and its association with RA in a western Mexican population. An analytical cross-sectional study was performed, in which 350 patients with RA and 307 CS were included. The identification of IL1RN VNTR polymorphism was carried out by polymerase chain reaction (PCR), and genotypes were associated with clinical variables (DAS28 and CRP). The presence of A1/A2 genotype was associated with RA risk (p = 0.03, OR = 1.45, 95% CI = 1.02–2.05). Also, results indicate that the presence of heterozygote genotypes which include A2 was associated with RA risk (p = 0.01, OR = 1.5, 95% CI = 1.07–2.11). Patients carrier of A2/A2 genotype have a higher score of DAS28 (5.64 [4.49–6.70]). A-/A- has higher level of CRP (2.30 [0.62–9.10]) in comparison with A2/A- (1.06 [0.37–2.82]). A1/A2 genotype was associated with susceptibility to RA in a western Mexican population. The presence of the A2/A2 genotype in RA is associated with increased disease activity.

ACS Style

S. Ramírez-Pérez; M. Salazar-Páramo; S. Pineda-Monjarás; U. De la Cruz-Mosso; Jorge Hernández-Bello; G. E. Martínez-Bonilla; A. L. Pereira-Suárez; J. F. Muñoz-Valle. Association of 86 bp variable number of tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL1RN) with susceptibility and clinical activity in rheumatoid arthritis. Clinical Rheumatology 2017, 36, 1247 -1252.

AMA Style

S. Ramírez-Pérez, M. Salazar-Páramo, S. Pineda-Monjarás, U. De la Cruz-Mosso, Jorge Hernández-Bello, G. E. Martínez-Bonilla, A. L. Pereira-Suárez, J. F. Muñoz-Valle. Association of 86 bp variable number of tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL1RN) with susceptibility and clinical activity in rheumatoid arthritis. Clinical Rheumatology. 2017; 36 (6):1247-1252.

Chicago/Turabian Style

S. Ramírez-Pérez; M. Salazar-Páramo; S. Pineda-Monjarás; U. De la Cruz-Mosso; Jorge Hernández-Bello; G. E. Martínez-Bonilla; A. L. Pereira-Suárez; J. F. Muñoz-Valle. 2017. "Association of 86 bp variable number of tandem repeat (VNTR) polymorphism of interleukin-1 receptor antagonist (IL1RN) with susceptibility and clinical activity in rheumatoid arthritis." Clinical Rheumatology 36, no. 6: 1247-1252.

Journal article
Published: 01 July 2015 in Human Immunology
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Primary Sjögren's syndrome (pSS) is an autoimmune disease characterized by lymphocytic infiltration of salivary and lacrimal glands. Interleukin-10 (IL-10) plays a role in autoimmune diseases by promoting B-cell activation and autoantibodies production. IL10-1082A>G, -819C>T, -592C>A polymorphisms and their haplotypes have been associated with IL-10 production. The aim of this study was to associate IL10 haplotypes with mRNA expression and soluble IL-10 levels with susceptibility to pSS in 111 Mexican patients and 111 healthy subjects (HS). Primary Sjögren's syndrome patients showed high levels of sIL-10 (p=0.0001 vs HS) correlating with anti-Ro and anti-La antibodies (p<0.05). In addition, IL10 mRNA expression in pSS was higher than HS (0.8 vs 0.1, p=0.1537). However, no difference was observed in sIL-10 levels between haplotypes. Patients carriers of GCC haplotype showed higher mRNA expression than ACC+ATA (1.4 vs 0.6, p=0.2424) and high foci number (p=0.04 vs ACC). Our results suggest a strong relationship of IL10 with pSS which is demonstrated by the increased mRNA expression and also high sIL-10 levels positively correlated with autoantibodies. Besides that, the GCC haplotype carriers expressed high mRNA. However, IL10 haplotypes were not associated with sIL-10 in pSS from Western Mexico which suggest that diverse biological factors may regulate the IL10 expression in pSS.

ACS Style

M. Vázquez-Villamar; C.A. Palafox-Sánchez; J.F. Muñoz-Valle; Y. Valle; G. Orozco-Barocio; Jorge Hernández-Bello; E. Oregon-Romero. Analysis of IL10 haplotypes in primary Sjögren’s syndrome patients from Western Mexico: Relationship with mRNA expression, IL-10 soluble levels, and autoantibodies. Human Immunology 2015, 76, 473 -479.

AMA Style

M. Vázquez-Villamar, C.A. Palafox-Sánchez, J.F. Muñoz-Valle, Y. Valle, G. Orozco-Barocio, Jorge Hernández-Bello, E. Oregon-Romero. Analysis of IL10 haplotypes in primary Sjögren’s syndrome patients from Western Mexico: Relationship with mRNA expression, IL-10 soluble levels, and autoantibodies. Human Immunology. 2015; 76 (7):473-479.

Chicago/Turabian Style

M. Vázquez-Villamar; C.A. Palafox-Sánchez; J.F. Muñoz-Valle; Y. Valle; G. Orozco-Barocio; Jorge Hernández-Bello; E. Oregon-Romero. 2015. "Analysis of IL10 haplotypes in primary Sjögren’s syndrome patients from Western Mexico: Relationship with mRNA expression, IL-10 soluble levels, and autoantibodies." Human Immunology 76, no. 7: 473-479.

Research article
Published: 20 April 2015 in Disease Markers
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We analyzed the relationship of −794CATT5–8and −173 G>CMIFpolymorphisms with mRNA and soluble MIF in young obese subjects. A total of 250 young subjects, 150 normal-weight and 100 obese subjects, were recruited in the study. Genotyping of −794CATT5–8and −173 G>CMIFpolymorphisms was performed by PCR and PCR-RFLP, respectively. MIF mRNA expression was determined by real-time PCR and serum MIF levels were measured using an ELISA kit. For bothMIFpromoter polymorphisms, no significant differences in the genotype and allele frequencies between groups were observed. MIF mRNA expression was slightly higher in obese subjects than in normal-weight subjects (1.38-fold), while soluble MIF levels did not show differences between groups. In addition, we found an increase in MIF mRNA expression in carriers of the 6,6 and C/C genotypes and the 6G haplotype of the −794CATT5–8and −173 G>CMIFpolymorphisms, although it was not significant. In conclusion, this study found no relationship between obesity andMIFgene promoter polymorphisms with MIF mRNA expression in young obese subjects.

ACS Style

Ines Matia-García; Lorenzo Salgado-Goytia; José F. Muñoz-Valle; Samuel García-Arellano; Jorge Hernández-Bello; Aralia B. Salgado-Bernabé; Isela Parra-Rojas. Macrophage Migration Inhibitory Factor Promoter Polymorphisms (−794 CATT5–8and −173 G>C): Relationship with mRNA Expression and Soluble MIF Levels in Young Obese Subjects. Disease Markers 2015, 2015, 1 -11.

AMA Style

Ines Matia-García, Lorenzo Salgado-Goytia, José F. Muñoz-Valle, Samuel García-Arellano, Jorge Hernández-Bello, Aralia B. Salgado-Bernabé, Isela Parra-Rojas. Macrophage Migration Inhibitory Factor Promoter Polymorphisms (−794 CATT5–8and −173 G>C): Relationship with mRNA Expression and Soluble MIF Levels in Young Obese Subjects. Disease Markers. 2015; 2015 ():1-11.

Chicago/Turabian Style

Ines Matia-García; Lorenzo Salgado-Goytia; José F. Muñoz-Valle; Samuel García-Arellano; Jorge Hernández-Bello; Aralia B. Salgado-Bernabé; Isela Parra-Rojas. 2015. "Macrophage Migration Inhibitory Factor Promoter Polymorphisms (−794 CATT5–8and −173 G>C): Relationship with mRNA Expression and Soluble MIF Levels in Young Obese Subjects." Disease Markers 2015, no. : 1-11.