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Microbiological safety in food industry are always a concern regarding sublethal tolerance in bacteria for common and natural sanitizers. Natural bacteriocins, such as nisin (NIS), may negatively interfere in the efficiency of major compounds of essential oils against foodborne pathogenic bacteria. However, nanoemulsioned forms increase the bactericidal potential of natural compounds acting synergistically. In this study, cinnamaldehyde (CIN), citral (CIT), and linalool (LIN) were evaluated independently, associated with NIS, and in nanoemulsions (NEs) against Bacillus cereus using untargeted-metabolomics. Results revealed morphological changes in the structure of B. cereus treated with NEs of CIN and CIT, both NIS-associated. In addition, sensibility tests and UHPLC-QTOF-MS analyses indicated that NIS might react together with CIT reducing the bactericidal efficiency, while the nanoemulsion of CIT effect was enhanced by NIS in nanoemulsioned forms. This study highlights the importance of prudent administration of natural compounds as antimicrobial agents to prevent sublethal tolerance in pathogenic bacteria.
Jorge Pamplona Pagnossa; Gabriele Rocchetti; Heloísa Helena De Abreu Martins; Jadson Diogo Pereira Bezerra; Gaber El-Saber Batiha; Eman A. El-Masry; Pier Sandro Cocconcelli; Cledir Santos; Luigi Lucini; Roberta Hilsdorf Piccoli. Morphological and metabolomics impact of sublethal doses of natural compounds and its nanoemulsions in Bacillus cereus. Food Research International 2021, 110658 .
AMA StyleJorge Pamplona Pagnossa, Gabriele Rocchetti, Heloísa Helena De Abreu Martins, Jadson Diogo Pereira Bezerra, Gaber El-Saber Batiha, Eman A. El-Masry, Pier Sandro Cocconcelli, Cledir Santos, Luigi Lucini, Roberta Hilsdorf Piccoli. Morphological and metabolomics impact of sublethal doses of natural compounds and its nanoemulsions in Bacillus cereus. Food Research International. 2021; ():110658.
Chicago/Turabian StyleJorge Pamplona Pagnossa; Gabriele Rocchetti; Heloísa Helena De Abreu Martins; Jadson Diogo Pereira Bezerra; Gaber El-Saber Batiha; Eman A. El-Masry; Pier Sandro Cocconcelli; Cledir Santos; Luigi Lucini; Roberta Hilsdorf Piccoli. 2021. "Morphological and metabolomics impact of sublethal doses of natural compounds and its nanoemulsions in Bacillus cereus." Food Research International , no. : 110658.
Gallbladder cancer (GBC) is one of the most common sites for biliary tract cancers. It has a worldwide distribution being endemic in South America and Southern Asia. These high GBC rates have previously been linked to the determinants of health such as nutrition, genetics, lifestyle, and environment. Exposure to aflatoxin B1 (AFB1), a human carcinogen, is suggested to be involved with GBC development. This work aims to analyse the interplay of social, lifestyle, and genetic predisposing factors to GBC. AFB1 plays a pivotal role in carcinogenic onset by genetic and epigenetic modifications. AFB1 can induce molecular changes involved in the GBC pathogenesis, such as overexpression of UCHL1 gene, mutagenesis of TP53 gene, abnormal expression of oncogenes BCL-2, and aberrantly methylation of ERBB family receptors. However, a large-scale scientific cooperation is needed to confirm these molecular links through which AFB1 may increase the GBC risk. For that, monitoring AFB1 exposure through AF-albumin and AFB1-lysine will clarify the level of exposure of the population to AFB1 in the GBC hotspot. Further, analyses of AFB1-adduct concentrations in GBC cases (fatal and non-fatal) are needed to understanding if AF contamination can trigger gallbladder cancer.
Jéssica Costa; Nelson Lima; Cledir Santos. An overview on possible links between aflatoxin B1 exposure and gallbladder cancer. Mycotoxin Research 2021, 37, 205 -214.
AMA StyleJéssica Costa, Nelson Lima, Cledir Santos. An overview on possible links between aflatoxin B1 exposure and gallbladder cancer. Mycotoxin Research. 2021; 37 (3):205-214.
Chicago/Turabian StyleJéssica Costa; Nelson Lima; Cledir Santos. 2021. "An overview on possible links between aflatoxin B1 exposure and gallbladder cancer." Mycotoxin Research 37, no. 3: 205-214.
COVID-19 and arboviruses (ARBOD) epidemics co-occurrence is a great concern. In tropical and subtropical regions, ARBOD diseases such as chikungunya, dengue, and Zika are frequent. In both COVID-19 and ARBOD cases, an accurate diagnosis of infected patients is crucial to promote adequate treatment and isolation measures in COVID-19 cases. Overlap of clinical symptoms and laboratory parameters between COVID-19 and ARBOD present themselves as an extra challenge during diagnosis. COVID-19 diagnosis is mainly performed by quantitative reverse polymerase chain reaction (RT-qPCR), while ARBOD diagnosis is performed by serology, detection of antigen or antibody, and molecular diagnosis. In this review, the epidemiologic profile of arboviruses and SARS-CoV-2 is analyzed, and potential risks of symptom overlap is addressed. The implementation of an analytical platform based on infrared (IR) spectroscopy, MALDI-TOF mass spectrometry, and RT-qPCR is discussed as an efficient strategy for a fast, robust, reliable, and cost-effective diagnosis system even during the co-occurrence of virus outbreaks. The spectral data of IR spectroscopy and MALDI-TOF MS obtained from COVID-19 infected and recovered patients can be used to build up an integrated spectral database. This approach can enable us to determine quickly the groups that have been exposed and have recovered from COVID-19 or ARBOD, avoiding misdiagnoses.
Jéssica Costa; Eugénio Ferreira; Cledir Santos. COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control. Microorganisms 2021, 9, 708 .
AMA StyleJéssica Costa, Eugénio Ferreira, Cledir Santos. COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control. Microorganisms. 2021; 9 (4):708.
Chicago/Turabian StyleJéssica Costa; Eugénio Ferreira; Cledir Santos. 2021. "COVID-19, Chikungunya, Dengue and Zika Diseases: An Analytical Platform Based on MALDI-TOF MS, IR Spectroscopy and RT-qPCR for Accurate Diagnosis and Accelerate Epidemics Control." Microorganisms 9, no. 4: 708.
BACKGROUND Fermented cocoa beans (Theobroma cacao L.) is a pivotal raw material for chocolate production. A cocktail yeast applied in the cocoa fermentation process can promote the formation of the pleasant metabolites. Saccharomyces, Pichia, and Hanseniaspora have been widely used in fermentation to improve the final product organoleptic profile. Highlighting that fermentation is a critical point for chocolate flavour precursors production. This study aims to evaluate the impact of Pichia kluyveri and Saccharomyces cerevisiae strains as starters cultures on the two cocoa hybrids fermentation FA13 and CEPEC2002. RESULTS During fermentation processes, volatile organic compounds (VOCs) and protein profiles were assessed. Chocolates produced were also assessed regarding the presence of VOCs. Eighty VOCs were identified by gas chromatography coupled to mass spectrometry analysis (HS‐SPME GC‐MS). Mass spectrometry by the MALDI‐TOF technique provided the protein profiles evolution over fermentation and showed they changed with inoculation type (spontaneous vs. inoculated fermentation). Chocolate obtained by FA13 inoculated with S. cerevisiae strain contained higher organics acids amount, being categorised sourer than chocolate produced by spontaneous fermentation of FA13. CEPEC2002 inoculated with S. cerevisiae strain in co‐culture with P. kluyveri strain generated less sour and sweeter chocolate than spontaneous fermentation only. CONCLUSION Chocolates from inoculated assays with starter cultures were more accepted by evaluators. Highlighting that P. kluyveri and S. cerevisiae influence the composition of VOCs. Besides, protein profiles also changed throughout fermentation. Further investigation should be conducted to clarify protein degradation dynamics during inoculated fermentations to defining which of both microbial cultures positively affect the chocolate sensory characteristics.
Igor Moreira; Jéssica Costa; Leonardo Vilela; Nelson Lima; Cledir Santos; Rosane Schwan. Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour. Journal of the Science of Food and Agriculture 2021, 101, 4409 -4419.
AMA StyleIgor Moreira, Jéssica Costa, Leonardo Vilela, Nelson Lima, Cledir Santos, Rosane Schwan. Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour. Journal of the Science of Food and Agriculture. 2021; 101 (10):4409-4419.
Chicago/Turabian StyleIgor Moreira; Jéssica Costa; Leonardo Vilela; Nelson Lima; Cledir Santos; Rosane Schwan. 2021. "Influence of S. cerevisiae and P. kluyveri as starters on chocolate flavour." Journal of the Science of Food and Agriculture 101, no. 10: 4409-4419.
This work aims to provide the first study on the mycobiota present in Chilean pepper Capsicum annuum L. cv. “Cacho de Cabra” throughout the early production stages. Two hundred and forty berry fruits were sampled: 1) at the ripe fruits harvest day; 2) during drying; and 3) smoking processes. A total of 192 strains, encompassing 11 genera and 44 species, were identified through analysis of β-tubulin (benA) gene and internal transcribed spacer of ribosomal DNA (ITS) region. All collection points showed samples with high fungal contamination, but the mycobiota composition varied as a result of different environmental conditions. Alternaria spp. and Fusarium spp. were predominantly isolated from fresh fruits of C. annuum. Penicillium spp. was the most frequent genus in all analysed points. Penicillium brevicompactum and P. crustosum were the most abundant species. Among Aspergillus, A. niger and A. flavus were dominant after the drying phase. In our study, none of the analysed strains of Penicillium (113) and Aspergillus (35) produced Ochratoxin A at detectable levels. The broad characterization of the fungal community of C. annuum carried out in this study, could be a guideline for future mycotoxin analyses performed directly on the pod. Understanding the role and dynamics of mycobiota and its relationship with the toxins present in this substrate, will be useful to establish and improve control measures considering the specificities of each point in the C. annuum production chain.
Jéssica Costa; Rodrigo Rodríguez; Carla Santos; Célia Soares; Nelson Lima; Cledir Santos. Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology 2020, 334, 108833 .
AMA StyleJéssica Costa, Rodrigo Rodríguez, Carla Santos, Célia Soares, Nelson Lima, Cledir Santos. Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén. International Journal of Food Microbiology. 2020; 334 ():108833.
Chicago/Turabian StyleJéssica Costa; Rodrigo Rodríguez; Carla Santos; Célia Soares; Nelson Lima; Cledir Santos. 2020. "Mycobiota in Chilean chilli Capsicum annuum L. used for production of Merkén." International Journal of Food Microbiology 334, no. : 108833.
Guarana plant is a native of the Amazon region. Due to its high amount of caffeine and tannins, the seed has medicinal and stimulating properties. The guarana industry has grown exponentially in recent years; however, little information is available about associated mycobiota, particularly endophytic fungi. The present study aimed to compare the distribution and diversity of endophytic fungi associated with the leaves and seeds of anthracnose-resistant and susceptible guarana plants produced in Maués and Manaus, Amazonas State, Brazil. A total of 7514 endophytic fungi were isolated on Potato Dextrose Agar, Sabouraud and Czapek media, and grouped into 77 morphological groups. Overall, fungal communities in guarana leaves and seeds were mainly composed by Colletotrichum and Fusarium genera, but also by Chondrostereum, Clonostachys, Curvularia, Hypomontagnella, Lentinus, Neopestalotiopsis, Nigrospora, Peroneutypa, Phyllosticta, Simplicillium and Tinctoporellus. Obtained results indicate that some members of Colletotrichum and Fusarium genera may have experienced dysbiosis during the guarana domestication process, suggesting that some individuals may behave as latent pathogens. The susceptible guarana genotype cultivated in Manaus presented higher fungal diversity. The relative abundance of taxa and diversity among samples suggests that communities are structured by genotype and geographic location. This is the first report of mycobiota in both guarana leaves and seeds.
Carla Santos; Blenda Naara Santos Da Silva; Ana Francisca Tibúrcia Amorim Ferreira E Ferreira; Cledir Santos; Nelson Lima; Jânia Lília Da Silva Bentes. Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin. Journal of Fungi 2020, 6, 123 .
AMA StyleCarla Santos, Blenda Naara Santos Da Silva, Ana Francisca Tibúrcia Amorim Ferreira E Ferreira, Cledir Santos, Nelson Lima, Jânia Lília Da Silva Bentes. Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin. Journal of Fungi. 2020; 6 (3):123.
Chicago/Turabian StyleCarla Santos; Blenda Naara Santos Da Silva; Ana Francisca Tibúrcia Amorim Ferreira E Ferreira; Cledir Santos; Nelson Lima; Jânia Lília Da Silva Bentes. 2020. "Fungal Endophytic Community Associated with Guarana (Paullinia cupana Var. Sorbilis): Diversity Driver by Genotypes in the Centre of Origin." Journal of Fungi 6, no. 3: 123.
This study describes a novel fungal species belonging to the genus Gongronella. During a previous work focusing on metalaxyl degradation by Mucorales strains, two isolates from vineyard soil samples collected in the Alentejo region, south Portugal, were identified as a putative novel species based on combined molecular and MALDI-TOF MS data. This new species is described here using a polyphasic approach that combines morphology, internal transcribed spacer of ribosomal DNA (ITS) and 28S ribosomal DNA (LSU) sequence data analysis and proteomic profiling by MALDI-TOF MS. Phenotypic and molecular data enabled this novel species to be clearly distinguished from other Gongronella species with results of combined ITS+LSU analysis showing that the Gongronella species is related to Gongronella butleri and Gongronella brasiliensis. Therefore, from the results of morphological and molecular analyses, isolates MUM 10.262 and MUM 10.263 seem to represent a new Gongronella species and the name Gongronella eborensis sp. nov. is proposed, with the ex-type strain MUM 10.262 (=CCMI 1100=CBS 128763).
Maria Rosário Martins; Carla Santos; Célia Soares; Cledir Santos; Nelson Lima. Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal). International Journal of Systematic and Evolutionary Microbiology 2020, 70, 3475 -3482.
AMA StyleMaria Rosário Martins, Carla Santos, Célia Soares, Cledir Santos, Nelson Lima. Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal). International Journal of Systematic and Evolutionary Microbiology. 2020; 70 (5):3475-3482.
Chicago/Turabian StyleMaria Rosário Martins; Carla Santos; Célia Soares; Cledir Santos; Nelson Lima. 2020. "Gongronella eborensis sp. nov., from vineyard soil of Alentejo (Portugal)." International Journal of Systematic and Evolutionary Microbiology 70, no. 5: 3475-3482.
The aim of this study was to compare the performance of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), phenotypic and molecular methods for the identification of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo (Brazil) between the years 2001 and 2017. Sequencing of ITS region of ribosomal DNA and elongation factor 1 alpha gene (ET1α) were used as reference method in the analysis of a total of 108 Fusarium spp. clinical strains isolated from human hosts with superficial and systemic infections. Agreement between MALDI-TOF-MS and molecular data was observed for 97 out of 108 clinical isolates (89.8%), whereas five (4.6%) and six (5.5%) clinical isolates were misidentified and were not identified by MALDI-TOF MS, respectively. ITS region sequences and MALDI-TOF MS mass spectra identified and grouped correctly most of Fusarium clinical isolates at species complex level. This investigation highlights the potential of MALDI-TOF MS technique as a fast and cost-efficient alternative for clinical Fusarium identification. However, MALDI-TOF MS requires a more accurate and larger database. This work is the first comprehensive report for Fusarium population, based on phenotypic analyses, proteomic profile by MALDI-TOF and phylogenetic analyses of Fusarium species complexes isolated from clinical cases in the State of Sao Paulo, Brazil.
Mario Henrique Paziani; Ludmilla Tonani Carvalho; Marcia De Souza Carvalho Melhem; Margarete Teresa Gottardo De Almeida; Maria Emilia Nadaletto Bonifácio Da Silva; Roberto Martinez; Cledir Santos; Marcia Regina Von Zeska Kress. First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology. Microorganisms 2019, 8, 66 .
AMA StyleMario Henrique Paziani, Ludmilla Tonani Carvalho, Marcia De Souza Carvalho Melhem, Margarete Teresa Gottardo De Almeida, Maria Emilia Nadaletto Bonifácio Da Silva, Roberto Martinez, Cledir Santos, Marcia Regina Von Zeska Kress. First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology. Microorganisms. 2019; 8 (1):66.
Chicago/Turabian StyleMario Henrique Paziani; Ludmilla Tonani Carvalho; Marcia De Souza Carvalho Melhem; Margarete Teresa Gottardo De Almeida; Maria Emilia Nadaletto Bonifácio Da Silva; Roberto Martinez; Cledir Santos; Marcia Regina Von Zeska Kress. 2019. "First Comprehensive Report of Clinical Fusarium Strains Isolated in the State of Sao Paulo (Brazil) and Identified by MALDI-TOF MS and Molecular Biology." Microorganisms 8, no. 1: 66.
Sporotrichosis is a globally distributed subcutaneous fungal infection caused by dimorphic fungi belonging to the Sporothrix species complex that affects the skin of limbs predominantly, but not exclusively. A rare case of ocular sporotrichosis in an immunocompetent Brazilian patient from the countryside of Rio de Janeiro State is reported. A 68-year-old woman presented with a subconjunctival infiltrative lesion in the right eye with pre-auricular lymphadenopathy of onset 4 months ago that evolved to suppurative nodular lesions on the eyelids. Conjunctival secretion was evaluated by histopathological examination and inoculated on Sabouraud Dextrose Agar (SDA). Histopathology showed oval bodies within giant cells and other mononucleated histiocytes. Fungus grown on SDA was identified as Sporothrix sp. by morphological observations. The isolated strain was finally identified by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) associated with an in-house database enriched with reference Sporothrix complex spectra. The strain presented a MALDI spectrum with the ion peaks of the molecular mass profile of S. brasiliensis. The patient was adequately treated with amphotericin B subsequently replaced by itraconazole. Due to scars left by the suppurative process, the patient presented poor final visual acuity. The present work presents an overview of ocular sporotrichosis and discusses the diagnostic difficulty that can lead to visual sequelae in these cases.
Aline M. F. Matos; Lucas M. Moreira; Bianca F. Barczewski; Lucas X. De Matos; Jordane B. V. De Oliveira; Maria Ines F. Pimentel; Rodrigo Almeida-Paes; Murilo G. Oliveira; Tatiana C. A. Pinto; Nelson Lima; Magnum De O. Matos; Louise G. De M. E Costa; Cledir Santos; Manoel Marques Evangelista Oliveira. Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient. Microorganisms 2019, 8, 22 .
AMA StyleAline M. F. Matos, Lucas M. Moreira, Bianca F. Barczewski, Lucas X. De Matos, Jordane B. V. De Oliveira, Maria Ines F. Pimentel, Rodrigo Almeida-Paes, Murilo G. Oliveira, Tatiana C. A. Pinto, Nelson Lima, Magnum De O. Matos, Louise G. De M. E Costa, Cledir Santos, Manoel Marques Evangelista Oliveira. Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient. Microorganisms. 2019; 8 (1):22.
Chicago/Turabian StyleAline M. F. Matos; Lucas M. Moreira; Bianca F. Barczewski; Lucas X. De Matos; Jordane B. V. De Oliveira; Maria Ines F. Pimentel; Rodrigo Almeida-Paes; Murilo G. Oliveira; Tatiana C. A. Pinto; Nelson Lima; Magnum De O. Matos; Louise G. De M. E Costa; Cledir Santos; Manoel Marques Evangelista Oliveira. 2019. "Identification by MALDI-TOF MS of Sporothrix brasiliensis Isolated from a Subconjunctival Infiltrative Lesion in an Immunocompetent Patient." Microorganisms 8, no. 1: 22.
This study aims to evaluate the effect of freeze-drying and long-term storage on the biotechnological potential of Aspergillus section Nigri strains. Twelve selected strains were freeze-dried and aged by accelerated storage, at 37 °C in the dark, for 2 and 4 weeks. To assess possible changes as a consequence of the ageing in the freeze-drying ampoules, morphological characteristics, mycotoxins and enzymes production, matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALTI-TOF MS) spectra, and M13 phage probe fingerprinting were used as part of a polyphasic approach. Phenotypical changes were observed; nevertheless, they did not substantially affect the potential biotechnological use of these strains. The activity of hydrolytic enzymes (protease, carboxymethylcellulase, xylanase, pectinase and mannanase) was maintained or increased after freeze-drying. MALDI-TOF MS data originated spectra that grouped, for the majority of samples, according to strain independently of preservation time point. M13 profiles revealed the presence of some genetic polymorphisms after preservation. However, the three studied times still clustered for more than 50% of strains. Our results show that the studied strains maintain their biotechnological potential after preservation, with minimal phenotypic alterations. These findings provide evidence that freeze-drying preservation is a suitable option to preserve biotechnologically relevant aspergilli strains from section Nigri, and one should consider that the observed effects might be species/strain-dependent.
Rodrigo Rodriguez; Carla Santos; Marta F. Simões; Célia Soares; Cledir Santos; Nelson Lima. Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains. Microorganisms 2019, 7, 291 .
AMA StyleRodrigo Rodriguez, Carla Santos, Marta F. Simões, Célia Soares, Cledir Santos, Nelson Lima. Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains. Microorganisms. 2019; 7 (9):291.
Chicago/Turabian StyleRodrigo Rodriguez; Carla Santos; Marta F. Simões; Célia Soares; Cledir Santos; Nelson Lima. 2019. "Polyphasic, Including MALDI-TOF MS, Evaluation of Freeze-Drying Long-Term Preservation on Aspergillus (Section Nigri) Strains." Microorganisms 7, no. 9: 291.
Novel species of fungi described in this study include those from various countries as follows: Australia, Chaetomella pseudocircinoseta and Coniella pseudodiospyri on Eucalyptus microcorys leaves, Cladophialophora eucalypti, Teratosphaeria dunnii and Vermiculariopsiella dunnii on Eucalyptus dunnii leaves, Cylindrium grande and Hypsotheca eucalyptorum on Eucalyptus grandis leaves, Elsinoe salignae on Eucalyptus saligna leaves, Marasmius lebeliae on litter of regenerating subtropical rainforest, Phialoseptomonium eucalypti (incl. Phialoseptomonium gen. nov.) on Eucalyptus grandis × camaldulensis leaves, Phlogicylindrium pawpawense on Eucalyptus tereticornis leaves, Phyllosticta longicauda as an endophyte from healthy Eustrephus latifolius leaves, Pseudosydowia eucalyptorum on Eucalyptus sp. leaves, Saitozyma wallum on Banksia aemula leaves, Teratosphaeria henryi on Corymbia henryi leaves. Brazil, Aspergillus bezerrae, Backusella azygospora, Mariannaea terricola and Talaromyces pernambucoensis from soil, Calonectria matogrossensis on Eucalyptus urophylla leaves, Calvatia brasiliensis on soil, Carcinomyces nordestinensis on Bromelia antiacantha leaves, Dendryphiella stromaticola on small branches of an unidentified plant, Nigrospora brasiliensis on Nopalea cochenillifera leaves, Penicillium alagoense as a leaf endophyte on a Miconia sp., Podosordaria nigrobrunnea on dung, Spegazzinia bromeliacearum as a leaf endophyte on Tilandsia catimbauensis, Xylobolus brasiliensis on decaying wood. Bulgaria, Kazachstania molopis from the gut of the beetle Molops piceus. Croatia, Mollisia endocrystallina from a fallen decorticated Picea abies tree trunk. Ecuador, Hygrocybe rodomaculata on soil. Hungary, Alfoldia vorosii (incl.Alfoldia gen. nov.) from Juniperus communis roots, Kiskunsagia ubrizsyi (incl. Kiskunsagia gen. nov.) from Fumana procumbens roots. India, Aureobasidium tremulum as laboratory contaminant, Leucosporidium himalayensis and Naganishia indica from windblown dust on glaciers. Italy, Neodevriesia cycadicola on Cycas sp. leaves, Pseudocercospora pseudomyrticola on Myrtus communis leaves, Ramularia pistaciae on Pistacia lentiscus leaves, Neognomoniopsis quercina (incl. Neognomoniopsis gen. nov.) on Quercus ilex leaves. Japan, Diaporthe fructicola on Passiflora edulis × P. edulis f. flavicarpa fruit, Entoloma nipponicum on leaf litter in a mixed Cryptomeria japonica and Acer spp. forest. Macedonia, Astraeus macedonicus on soil. Malaysia, Fusicladium eucalyptigenum on Eucalyptus sp. twigs, Neoacrodontiella eucalypti (incl. Neoacrodontiella gen. nov.) on Eucalyptus urophylla leaves. Mozambique, Meliola gorongosensis on dead Philenoptera violacea leaflets. Nepal, Coniochaeta dendrobiicola from Dendriobium lognicornu roots. New Zealand, Neodevriesia sexualis and Thozetella neonivea on Archontophoenix cunninghamiana leaves. Norway, Calophoma sandfjordenica from a piece of board on a rocky shoreline, Clavaria parvispora on soil, Didymella finnmarkica from a piece of Pinus sylvestris driftwood. Poland, Sugiyamaella trypani from soil. Portugal, Colletotrichum feijoicola from Acca sellowiana. Russia, Crepidotus tobolensis on Populus tremula debris, Entoloma ekaterinae, Entoloma erhardii and Suillus gastroflavus on soil, Nakazawaea ambrosiae from the galleries of Ips typographus under the bark of Picea abies. Slovenia, Pluteus ludwigii on twigs of broadleaved trees. South Africa, Anungitiomyces stellenboschiensis (incl. Anungitiomyces gen. nov.) and Niesslia stellenboschiana on Eucalyptus sp. leaves, Beltraniella pseudoportoricensis on Podocarpus falcatus leaf litter, Corynespora encephalarti on Encephalartos sp. leaves, Cytospora pavettae on Pavetta revoluta leaves, Helminthosporium erythrinicola on Erythrina humeana leaves, Helminthosporium syzygii on a Syzygium sp. barkcanker, Libertasomyces aloeticus on Aloe sp. leaves, Penicillium lunae from Musa sp. fruit, Phyllosticta lauridiae on Lauridia tetragona leaves, Pseudotruncatella bolusanthi (incl. Pseudotruncatellaceae fam. nov.) and Dactylella bolusanthi on Bolusanthus speciosus leaves. Spain, Apenidiella foetida on submerged plant debris, Inocybe grammatoides on Quercus ilex subsp. ilex forest humus, Ossicaulis salomii on soil, Phialemonium guarroi from soil. Thailand, Pantospora chromolaenae on Chromolaena odorata leaves. Ukraine, Cadophora helianthi from Helianthus annuus stems. USA, Boletus pseudopinophilus on soil under slash pine, Botryotrichum foricae, Penicillium americanum and Penicillium minnesotense from air. Vietnam, Lycoperdon vietnamense on soil. Morphological and culture characteristics are supported by DNA barcodes.
P.W. Crous; A.J. Carnegie; M.J. Wingfield; Rohit Sharma; G. Mughini; M.E. Noordeloos; Alberto Santini; Y.S. Shouche; Jadson Diogo Pereira Bezerra; Bálint Dima; V. Guarnaccia; I. Imrefi; Ž. Jurjević; Dániel G. Knapp; Gabor M. Kovacs; Donato Magistà; Giancarlo Perrone; Teppo Rämä; Yury Rebriev; R.G. Shivas; S.M. Singh; C.M. Souza-Motta; R. Thangavel; N.N. Adhapure; A.V. Alexandrova; A.C. Alfenas; R.F. Alfenas; P. Alvarado; A.L. Alves; D.A. Andrade; J.P. Andrade; R.N. Barbosa; A. Barili; Christopher Barnes; I.G. Baseia; J.-M. Bellanger; C. Berlanas; A.E. Bessette; Alona Biketova; F.S. Bomfim; T.E. Brandrud; Kaylene Bransgrove; A.C.Q. Brito; J.F. Cano-Lira; T. Cantillo; A.D. Cavalcanti; R. Cheewangkoon; R.S. Chikowski; C. Conforto; Thalline Rafhaella Leite Cordeiro; J.D. Craine; R. Cruz; U. Damm; R.J.V. De Oliveira; J.T. De Souza; H.G. De Souza; J.D.W. Dearnaley; R.A. Dimitrov; Francesco Dovana; A. Erhard; F. Esteve-Raventós; C.R. Félix; G. Ferisin; R.A. Fernandes; R.J. Ferreira; L.O. Ferro; C.N. Figueiredo; J.L. Frank; K.T.L.S. Freire; D. García; J. Gené; A. Gęsiorska; T.B. Gibertoni; R.A.G. Gondra; D.E. Gouliamova; D. Gramaje; F. Guard; L.F.P. Gusmão; S. Haitook; Y. Hirooka; J. Houbraken; V. Hubka; Areeb Inamdar; T. Iturriaga; I. Iturrieta-González; M. Jadan; N. Jiang; A. Justo; Aleksey Kachalkin; V.I. Kapitonov; M. Karadelev; J. Karakehian; T. Kasuya; I. Kautmanová; J. Kruse; I. Kušan; T.A. Kuznetsova; Melissa Landell; K.-H. Larsson; H.B. Lee; D.X. Lima; C.R.S. Lira; A.R. Machado; H. Madrid; O.M.C. Magalhães; H. Majerova; E.F. Malysheva; R.R. Mapperson; P.A.S. Marbach; M.P. Martín; A. Martín-Sanz; N. Matočec; A.R. McTaggart; J.F. Mello; Roger Melo; A. Mešič; S.J. Michereff; A.N. Miller; A. Minoshima; Leire Molinero-Ruiz; O.V. Morozova; D. Mosoh; M. Nabe; R. Naik; K. Nara; S.S. Nascimento; R.P. Neves; I. Olariaga; T.G.L. Oliveira; T. Ono; M.E. Ordoñez; A. De M. Ottoni; L.M. Paiva; F. Pancorbo; B. Pant; Julia Pawłowska; S.W. Peterson; D.B. Raudabaugh; E. Rodríguez-Andrade; E. Rubio; Katerina Rusevska; A.L.C.M.A. Santiago; Ana Santos; Cledir Santos; N.A. Sazanova; S. Shah; J. Sharma; B.D.B. Silva; J.L. Siquier; M.S. Sonawane; A.M. Stchigel; T. Svetasheva; N. Tamakeaw; M.T. Telleria; Patricia Vieira Tiago; C.M. Tian; Zdenko Tkalcec; M.A. Tomashevskaya; H.H. Truong; M.V. Vecherskii; Cobus M Visagie; A. Vizzini; Neriman Yilmaz; I.V. Zmitrovich; E.A. Zvyagina; T. Boekhout; T. Kehlet; T. Læssøe; J.Z. Groenewald. Fungal Planet description sheets: 868–950. Persoonia - Molecular Phylogeny and Evolution of Fungi 2019, 42, 291 -473.
AMA StyleP.W. Crous, A.J. Carnegie, M.J. Wingfield, Rohit Sharma, G. Mughini, M.E. Noordeloos, Alberto Santini, Y.S. Shouche, Jadson Diogo Pereira Bezerra, Bálint Dima, V. Guarnaccia, I. Imrefi, Ž. Jurjević, Dániel G. Knapp, Gabor M. Kovacs, Donato Magistà, Giancarlo Perrone, Teppo Rämä, Yury Rebriev, R.G. Shivas, S.M. Singh, C.M. Souza-Motta, R. Thangavel, N.N. Adhapure, A.V. Alexandrova, A.C. Alfenas, R.F. Alfenas, P. Alvarado, A.L. Alves, D.A. Andrade, J.P. Andrade, R.N. Barbosa, A. Barili, Christopher Barnes, I.G. Baseia, J.-M. Bellanger, C. Berlanas, A.E. Bessette, Alona Biketova, F.S. Bomfim, T.E. Brandrud, Kaylene Bransgrove, A.C.Q. Brito, J.F. Cano-Lira, T. Cantillo, A.D. Cavalcanti, R. Cheewangkoon, R.S. Chikowski, C. Conforto, Thalline Rafhaella Leite Cordeiro, J.D. Craine, R. Cruz, U. Damm, R.J.V. De Oliveira, J.T. De Souza, H.G. De Souza, J.D.W. Dearnaley, R.A. Dimitrov, Francesco Dovana, A. Erhard, F. Esteve-Raventós, C.R. Félix, G. Ferisin, R.A. Fernandes, R.J. Ferreira, L.O. Ferro, C.N. Figueiredo, J.L. Frank, K.T.L.S. Freire, D. García, J. Gené, A. Gęsiorska, T.B. Gibertoni, R.A.G. Gondra, D.E. Gouliamova, D. Gramaje, F. Guard, L.F.P. Gusmão, S. Haitook, Y. Hirooka, J. Houbraken, V. Hubka, Areeb Inamdar, T. Iturriaga, I. Iturrieta-González, M. Jadan, N. Jiang, A. Justo, Aleksey Kachalkin, V.I. Kapitonov, M. Karadelev, J. Karakehian, T. Kasuya, I. Kautmanová, J. Kruse, I. Kušan, T.A. Kuznetsova, Melissa Landell, K.-H. Larsson, H.B. Lee, D.X. Lima, C.R.S. Lira, A.R. Machado, H. Madrid, O.M.C. Magalhães, H. Majerova, E.F. Malysheva, R.R. Mapperson, P.A.S. Marbach, M.P. Martín, A. Martín-Sanz, N. Matočec, A.R. McTaggart, J.F. Mello, Roger Melo, A. Mešič, S.J. Michereff, A.N. Miller, A. Minoshima, Leire Molinero-Ruiz, O.V. Morozova, D. Mosoh, M. Nabe, R. Naik, K. Nara, S.S. Nascimento, R.P. Neves, I. Olariaga, T.G.L. Oliveira, T. Ono, M.E. Ordoñez, A. De M. Ottoni, L.M. Paiva, F. Pancorbo, B. Pant, Julia Pawłowska, S.W. Peterson, D.B. Raudabaugh, E. Rodríguez-Andrade, E. Rubio, Katerina Rusevska, A.L.C.M.A. Santiago, Ana Santos, Cledir Santos, N.A. Sazanova, S. Shah, J. Sharma, B.D.B. Silva, J.L. Siquier, M.S. Sonawane, A.M. Stchigel, T. Svetasheva, N. Tamakeaw, M.T. Telleria, Patricia Vieira Tiago, C.M. Tian, Zdenko Tkalcec, M.A. Tomashevskaya, H.H. Truong, M.V. Vecherskii, Cobus M Visagie, A. Vizzini, Neriman Yilmaz, I.V. Zmitrovich, E.A. Zvyagina, T. Boekhout, T. Kehlet, T. Læssøe, J.Z. Groenewald. Fungal Planet description sheets: 868–950. Persoonia - Molecular Phylogeny and Evolution of Fungi. 2019; 42 (1):291-473.
Chicago/Turabian StyleP.W. Crous; A.J. Carnegie; M.J. Wingfield; Rohit Sharma; G. Mughini; M.E. Noordeloos; Alberto Santini; Y.S. Shouche; Jadson Diogo Pereira Bezerra; Bálint Dima; V. Guarnaccia; I. Imrefi; Ž. Jurjević; Dániel G. Knapp; Gabor M. Kovacs; Donato Magistà; Giancarlo Perrone; Teppo Rämä; Yury Rebriev; R.G. Shivas; S.M. Singh; C.M. Souza-Motta; R. Thangavel; N.N. Adhapure; A.V. Alexandrova; A.C. Alfenas; R.F. Alfenas; P. Alvarado; A.L. Alves; D.A. Andrade; J.P. Andrade; R.N. Barbosa; A. Barili; Christopher Barnes; I.G. Baseia; J.-M. Bellanger; C. Berlanas; A.E. Bessette; Alona Biketova; F.S. Bomfim; T.E. Brandrud; Kaylene Bransgrove; A.C.Q. Brito; J.F. Cano-Lira; T. Cantillo; A.D. Cavalcanti; R. Cheewangkoon; R.S. Chikowski; C. Conforto; Thalline Rafhaella Leite Cordeiro; J.D. Craine; R. Cruz; U. Damm; R.J.V. De Oliveira; J.T. De Souza; H.G. De Souza; J.D.W. Dearnaley; R.A. Dimitrov; Francesco Dovana; A. Erhard; F. Esteve-Raventós; C.R. Félix; G. Ferisin; R.A. Fernandes; R.J. Ferreira; L.O. Ferro; C.N. Figueiredo; J.L. Frank; K.T.L.S. Freire; D. García; J. Gené; A. Gęsiorska; T.B. Gibertoni; R.A.G. Gondra; D.E. Gouliamova; D. Gramaje; F. Guard; L.F.P. Gusmão; S. Haitook; Y. Hirooka; J. Houbraken; V. Hubka; Areeb Inamdar; T. Iturriaga; I. Iturrieta-González; M. Jadan; N. Jiang; A. Justo; Aleksey Kachalkin; V.I. Kapitonov; M. Karadelev; J. Karakehian; T. Kasuya; I. Kautmanová; J. Kruse; I. Kušan; T.A. Kuznetsova; Melissa Landell; K.-H. Larsson; H.B. Lee; D.X. Lima; C.R.S. Lira; A.R. Machado; H. Madrid; O.M.C. Magalhães; H. Majerova; E.F. Malysheva; R.R. Mapperson; P.A.S. Marbach; M.P. Martín; A. Martín-Sanz; N. Matočec; A.R. McTaggart; J.F. Mello; Roger Melo; A. Mešič; S.J. Michereff; A.N. Miller; A. Minoshima; Leire Molinero-Ruiz; O.V. Morozova; D. Mosoh; M. Nabe; R. Naik; K. Nara; S.S. Nascimento; R.P. Neves; I. Olariaga; T.G.L. Oliveira; T. Ono; M.E. Ordoñez; A. De M. Ottoni; L.M. Paiva; F. Pancorbo; B. Pant; Julia Pawłowska; S.W. Peterson; D.B. Raudabaugh; E. Rodríguez-Andrade; E. Rubio; Katerina Rusevska; A.L.C.M.A. Santiago; Ana Santos; Cledir Santos; N.A. Sazanova; S. Shah; J. Sharma; B.D.B. Silva; J.L. Siquier; M.S. Sonawane; A.M. Stchigel; T. Svetasheva; N. Tamakeaw; M.T. Telleria; Patricia Vieira Tiago; C.M. Tian; Zdenko Tkalcec; M.A. Tomashevskaya; H.H. Truong; M.V. Vecherskii; Cobus M Visagie; A. Vizzini; Neriman Yilmaz; I.V. Zmitrovich; E.A. Zvyagina; T. Boekhout; T. Kehlet; T. Læssøe; J.Z. Groenewald. 2019. "Fungal Planet description sheets: 868–950." Persoonia - Molecular Phylogeny and Evolution of Fungi 42, no. 1: 291-473.
Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases
Matheus Sanitá Lima; Rosymar Coutinho de Lucas; Nelson Lima; Maria De Lourdes Teixeira De Moraes Polizeli; Cledir Santos. Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases. Frontiers in Microbiology 2019, 10, 315 .
AMA StyleMatheus Sanitá Lima, Rosymar Coutinho de Lucas, Nelson Lima, Maria De Lourdes Teixeira De Moraes Polizeli, Cledir Santos. Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases. Frontiers in Microbiology. 2019; 10 ():315.
Chicago/Turabian StyleMatheus Sanitá Lima; Rosymar Coutinho de Lucas; Nelson Lima; Maria De Lourdes Teixeira De Moraes Polizeli; Cledir Santos. 2019. "Fungal Community Ecology Using MALDI-TOF MS Demands Curated Mass Spectral Databases." Frontiers in Microbiology 10, no. : 315.
Capsicum products are widely commercialised and consumed worldwide. These substrates present unusual nutritional characteristics for microbial growth. Despite this, the presence of spoilage fungi and the co-occurrence of mycotoxins in the pepper production chain have been commonly detected. The main aim of this work was to review the critical control points, with a focus on mycotoxin contamination, during the production, storage and distribution of Capsicum products from a safety perspective; outlining the important role of ecophysiological factors in stimulating or inhibiting mycotoxin biosynthesis in these food commodities. Moreover, the human health risks caused by the ingestion of peppers contaminated with mycotoxins were also reviewed. Overall, Capsicum and its derivative-products are highly susceptible to contamination by mycotoxins. Pepper crop production and further transportation, processing and storage are crucial for production of safe food.
Jéssica Souza da Costa; Rodrigo Rodríguez; Esther Garcia-Cela; Angel Medina; Naresh Magan; Nelson Lima; Paola Battilani; Cledir Santos. Overview of Fungi and Mycotoxin Contamination in Capsicum Pepper and in Its Derivatives. Toxins 2019, 11, 27 .
AMA StyleJéssica Souza da Costa, Rodrigo Rodríguez, Esther Garcia-Cela, Angel Medina, Naresh Magan, Nelson Lima, Paola Battilani, Cledir Santos. Overview of Fungi and Mycotoxin Contamination in Capsicum Pepper and in Its Derivatives. Toxins. 2019; 11 (1):27.
Chicago/Turabian StyleJéssica Souza da Costa; Rodrigo Rodríguez; Esther Garcia-Cela; Angel Medina; Naresh Magan; Nelson Lima; Paola Battilani; Cledir Santos. 2019. "Overview of Fungi and Mycotoxin Contamination in Capsicum Pepper and in Its Derivatives." Toxins 11, no. 1: 27.
Cocoa beans from different geographical and genetic origins show distinct fermentation dynamics which result in different chocolate qualities. In order to understand the effects of genetic improvement of cocoa plants, in this work volatile compounds and proteins profiles of beginning and end of the fermentation from different cocoa hybrids (CEPEC2004, PH15, PS1319, SJ02) were searched. Moreover, sensorial characterization of the produced chocolate from these hybrids was performed. According to the results obtained, different volatile compounds were identified in fermented beans and in the chocolate produced. Chocolate from CEPEC2004 was the most accepted by judges and correlated with sweet and bitter taste which can be explained by the presence of desired flavor compounds, such as 2,3-butanediol and 2-methyl-1-butanol. A higher presence of acids (undesirable compounds) was observed in chocolates samples from PS1319 hybrid, that have resulted in the low acceptance by judges. In addition, MALDI-TOF MS analysis showed that during fermentation the protein profile was different among the hybrids, which indicates this kind of compounds also contributes to the cocoa-specific flavor.
Igor Magalhães Da Veiga Moreira; Leonardo De Figueiredo Vilela; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. Volatile compounds and protein profiles analyses of fermented cocoa beans and chocolates from different hybrids cultivated in Brazil. Food Research International 2018, 109, 196 -203.
AMA StyleIgor Magalhães Da Veiga Moreira, Leonardo De Figueiredo Vilela, Cledir Santos, Nelson Lima, Rosane Freitas Schwan. Volatile compounds and protein profiles analyses of fermented cocoa beans and chocolates from different hybrids cultivated in Brazil. Food Research International. 2018; 109 ():196-203.
Chicago/Turabian StyleIgor Magalhães Da Veiga Moreira; Leonardo De Figueiredo Vilela; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. 2018. "Volatile compounds and protein profiles analyses of fermented cocoa beans and chocolates from different hybrids cultivated in Brazil." Food Research International 109, no. : 196-203.
In this study, the degradation of metalaxyl was investigated in the presence of two Mucorales strains, previously isolated from soil subjected to repeated treatments with this fungicide and selected after enrichment technique. Fungal strains were characterised by a polyphasic approach using phylogenetic analysis of the Internal Transcribed Spacer (ITS) gene region, phenotypic characterisation by Matrix-Assisted Laser Desorption Ionization Time-Of-Flight Mass Spectrometry (MALDI-TOF MS) spectral analysis, and growth kinetics experiments. The strains were identified as Gongronella sp. and Rhizopus oryzae. The fungal growth kinetics in liquid cultures containing metalaxyl fits with Haldane model. Under laboratory conditions, the ability of Gongronella sp. and R. oryzae cultures to degrade metalaxyl was evaluated in liquid cultures and soil experiments. Both species were able to: (a) use metalaxyl as the main carbon and energy source; and (b) degrade metalaxyl in polluted soils, with rates around 1.0 mg kg−1 d−1. This suggests these strains could degrade metalaxyl in soils contaminated with this fungicide.
Maria Rosário Martins; Cledir Santos; Pablo Pereira; Júlio Cruz-Morais; Nelson Lima. Metalaxyl Degradation by Mucorales Strains Gongronella sp. and Rhizopus oryzae. Molecules 2017, 22, 2225 .
AMA StyleMaria Rosário Martins, Cledir Santos, Pablo Pereira, Júlio Cruz-Morais, Nelson Lima. Metalaxyl Degradation by Mucorales Strains Gongronella sp. and Rhizopus oryzae. Molecules. 2017; 22 (12):2225.
Chicago/Turabian StyleMaria Rosário Martins; Cledir Santos; Pablo Pereira; Júlio Cruz-Morais; Nelson Lima. 2017. "Metalaxyl Degradation by Mucorales Strains Gongronella sp. and Rhizopus oryzae." Molecules 22, no. 12: 2225.
Chocolate production suffered a vast impact with the emergence of the “witches’ broom” disease in cocoa plants. To recover cocoa production, many disease-resistant hybrid plants have been developed. However, some different cocoa hybrids produce cocoa beans that generate chocolate with variable quality. Fermentation of cocoa beans is a microbiological process that can be applied for the production of chocolate flavor precursors, leading to overcoming the problem of variable chocolate quality. The aim of this work was to use a cocktail of microorganisms as a starter culture on the fermentation of the ripe cocoa pods from PH15 cocoa hybrid, and evaluate its influence on the microbial communities present on the fermentative process on the compounds involved during the fermentation, and to perform the chocolate sensorial characterization. According to the results obtained, different volatile compounds were identified in fermented beans and in the chocolate produced. Bitterness was the dominant taste found in non-inoculated chocolate, while chocolate made with inoculated beans showed bitter, sweet, and cocoa tastes. 2,3-Butanediol and 2,3-dimethylpyrazine were considered as volatile compounds making the difference on the flavor of both chocolates. Saccharomyces cerevisiae UFLA CCMA 0200, Lactobacillus plantarum CCMA 0238, and Acetobacter pasteurianus CCMA 0241 are proposed as starter cultures for cocoa fermentation.
Igor Magalhães Da Veiga Moreira; Leonardo De Figueiredo Vilela; Maria Gabriela Da Cruz Pedroso Miguel; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor. Molecules 2017, 22, 766 .
AMA StyleIgor Magalhães Da Veiga Moreira, Leonardo De Figueiredo Vilela, Maria Gabriela Da Cruz Pedroso Miguel, Cledir Santos, Nelson Lima, Rosane Freitas Schwan. Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor. Molecules. 2017; 22 (5):766.
Chicago/Turabian StyleIgor Magalhães Da Veiga Moreira; Leonardo De Figueiredo Vilela; Maria Gabriela Da Cruz Pedroso Miguel; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. 2017. "Impact of a Microbial Cocktail Used as a Starter Culture on Cocoa Fermentation and Chocolate Flavor." Molecules 22, no. 5: 766.
The identification of clinically relevant fungi in the mycological laboratory has been attempted by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). MALDI-TOF MS in routine diagnostic clinical mycology is rare, especially for filamentous fungi, mainly due to extended sample preparation for filamentous fungi for good-quality spectra, low numbers of representative taxa in databases and the quality of databases. Modified protocols for sample preparation have been established by the main MALDI-TOF MS manufacturers for yeasts to improve spectral acquisition. Different studies have reported the establishment of in-house database sets within commercially available databases, MALDI Biotyper and VITEK MS, covering mainly a single fungal genus. Identification of clinically relevant filamentous fungi is based on the analysis of the whole fungal biomass of fungi such as dermatophytes. In this chapter, the authors evaluate 2,5-dihydroxybenzoic acid (DHB) and α-cyano-4-hydroxycinnamic acid (CHCA) matrices. They also evaluate different extraction protocols and previous suspension of cells in water or solvent mixtures.
Cledir R. Santos; Elaine Cristina Francisco; Mariana Mazza; Ana Carolina B. Padovan; Arnaldo Colombo; Nelson Lima. Impact of MALDI-TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics. MALDI-TOF and Tandem MS for Clinical Microbiology 2017, 211 -230.
AMA StyleCledir R. Santos, Elaine Cristina Francisco, Mariana Mazza, Ana Carolina B. Padovan, Arnaldo Colombo, Nelson Lima. Impact of MALDI-TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics. MALDI-TOF and Tandem MS for Clinical Microbiology. 2017; ():211-230.
Chicago/Turabian StyleCledir R. Santos; Elaine Cristina Francisco; Mariana Mazza; Ana Carolina B. Padovan; Arnaldo Colombo; Nelson Lima. 2017. "Impact of MALDI-TOF MS in Clinical Mycology; Progress and Barriers in Diagnostics." MALDI-TOF and Tandem MS for Clinical Microbiology , no. : 211-230.
Dynamic microbial over the cocoa fermentation using starter culture and the effect sensory characteristics of chocolate produced were investigated. The cocoa fermentation inoculated with Saccharomyces cerevisiae CCMA0681 and Lactobacillus fermentum UFLA CHBB 8.12 as starter cultures were assessed, and compared with spontaneous fermentation. The microbial succession was identified using polyphasic approach including classical morphological and biochemical assays, and Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF). Overall 873 colonies were isolated, 445 (51%) strains were isolated from the spontaneous fermentation, while 428 (49%) strains were isolated from the inoculated fermentation. The dominant yeast in both fermentation processes were S. cerevisiae and Candida magnolia. L. fermentum and Pediococcus acidilactici were detected in both fermentations, whereas L. coryniformis, L. curvatus, L. mali, L. plantarum, and L. sakei were isolated from the spontaneous fermentation only. Acinetobacter sp., Gluconobacter oxydans, and Acetobacter pasteurianus were isolated from both the fermentation processes. Chocolate produced from the spontaneous fermentative process presented dominance of the bitter flavour, while obtained through inoculated fermentation process presented bitter, astringent and acid as dominant flavours. Cocoa inoculation with S. cerevisiae and L. fermentum affected the sensory quality of the produced chocolate. The microbial inoculation influenced on fermentation and therefore the final product.The authors thank the following Brazilian agencies Conselho Nacional de Desenvolvimento Científico e Tecnológico of Brasil (CNPQ), Fundação de Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG), and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for scholarships, and Fazendas Reunidas Vale do Juliana (Igrapiúna, Bahia, Brazil) for sample collection
Maria Gabriela Da Cruz Pedrozo Miguel; Lívia Reis; Priscila Efraim; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. Cocoa fermentation: Microbial identification by MALDI-TOF MS, and sensory evaluation of produced chocolate. LWT 2017, 77, 362 -369.
AMA StyleMaria Gabriela Da Cruz Pedrozo Miguel, Lívia Reis, Priscila Efraim, Cledir Santos, Nelson Lima, Rosane Freitas Schwan. Cocoa fermentation: Microbial identification by MALDI-TOF MS, and sensory evaluation of produced chocolate. LWT. 2017; 77 ():362-369.
Chicago/Turabian StyleMaria Gabriela Da Cruz Pedrozo Miguel; Lívia Reis; Priscila Efraim; Cledir Santos; Nelson Lima; Rosane Freitas Schwan. 2017. "Cocoa fermentation: Microbial identification by MALDI-TOF MS, and sensory evaluation of produced chocolate." LWT 77, no. : 362-369.
Water contamination with large amounts of industrial textile coloured effluents is an environmental concern. For the treatment of textile effluents, white-rot fungi have received extensive attention due to their powerful capability to produce oxidative (e.g., ligninolytic) enzymes. In addition, other groups of fungi, such as species of Aspergillus and Trichoderma, have also been used for textile effluents treatment. The main aim of the present study was to requalify a Brazilian Trichoderma culture collection of 51 Trichoderma strains, isolated from different sources in Brazil and preserved in the oldest Latin-American Fungal Service Culture Collection, The Micoteca URM WDCM 804 (Recife, Brazil). Fungal isolates were re-identified through a polyphasic approach including macro- and micro-morphology and molecular biology, and screened for their capability to decolourise real effluents collected directly from storage tanks of a textile manufacture. Trichoderma atroviride URM 4950 presented the best performance on the dye decolourisation in real textile effluent and can be considered in a scale-up process at industrial level. Overall, the potential of Trichoderma strains in decolourising real textile dye present in textile effluent and the production of the oxidative enzymes Lac, LiP and MnP was demonstrated. Fungal strains are available in the collection e-catalogue to be further explored from the biotechnological point of view.
Dianny Silva Lisboa; Cledir Santos; Renan N. Barbosa; Oliane Magalhães; Laura M. Paiva; Keila A. Moreira; Nelson Lima; Cristina M. Souza-Motta. Requalification of a Brazilian Trichoderma Collection and Screening of Its Capability to Decolourise Real Textile Effluent. International Journal of Environmental Research and Public Health 2017, 14, 373 .
AMA StyleDianny Silva Lisboa, Cledir Santos, Renan N. Barbosa, Oliane Magalhães, Laura M. Paiva, Keila A. Moreira, Nelson Lima, Cristina M. Souza-Motta. Requalification of a Brazilian Trichoderma Collection and Screening of Its Capability to Decolourise Real Textile Effluent. International Journal of Environmental Research and Public Health. 2017; 14 (4):373.
Chicago/Turabian StyleDianny Silva Lisboa; Cledir Santos; Renan N. Barbosa; Oliane Magalhães; Laura M. Paiva; Keila A. Moreira; Nelson Lima; Cristina M. Souza-Motta. 2017. "Requalification of a Brazilian Trichoderma Collection and Screening of Its Capability to Decolourise Real Textile Effluent." International Journal of Environmental Research and Public Health 14, no. 4: 373.
Filamentous fungi (ff) are a diverse group of unique eukaryotic organisms currently accepted to belong to the Eumycota kingdom. They are ubiquitous in nature with an extraordinary ability to decompose plant wastes while also causing much spoilage of food commodities. Information about each ff, namely, morphological and molecular descriptions, including modern spectral data MALDI-TOF MS, physiological and biochemical features, ecological roles, and societal risks or benefits is the key element in fungal identification. In order to attain a rapid ff identification in food the genomic and proteomic approaches are currently used. The present paper focus on the main contribution of MALDI-TOF MS to identification spoilage ff in food.Nelson Lima acknowledges the support of “BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020-Programa Operacional Regional do Norte”
Nelson Lima; Cledir Santos. MALDI-TOF MS for identification of food spoilage filamentous fungi. Current Opinion in Food Science 2017, 13, 26 -30.
AMA StyleNelson Lima, Cledir Santos. MALDI-TOF MS for identification of food spoilage filamentous fungi. Current Opinion in Food Science. 2017; 13 ():26-30.
Chicago/Turabian StyleNelson Lima; Cledir Santos. 2017. "MALDI-TOF MS for identification of food spoilage filamentous fungi." Current Opinion in Food Science 13, no. : 26-30.