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Spyros Gkelis
Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece

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Journal article
Published: 24 May 2021 in Marine Drugs
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Sponges form symbiotic relationships with diverse and abundant microbial communities. Cyanobacteria are among the most important members of the microbial communities that are associated with sponges. Here, we performed a genus-wide comparative genomic analysis of the newly described marine benthic cyanobacterial genus Leptothoe (Synechococcales). We obtained draft genomes from Le. kymatousa TAU-MAC 1615 and Le. spongobia TAU-MAC 1115, isolated from marine sponges. We identified five additional Leptothoe genomes, host-associated or free-living, using a phylogenomic approach, and the comparison of all genomes showed that the sponge-associated strains display features of a symbiotic lifestyle. Le. kymatousa and Le. spongobia have undergone genome reduction; they harbored considerably fewer genes encoding for (i) cofactors, vitamins, prosthetic groups, pigments, proteins, and amino acid biosynthesis; (ii) DNA repair; (iii) antioxidant enzymes; and (iv) biosynthesis of capsular and extracellular polysaccharides. They have also lost several genes related to chemotaxis and motility. Eukaryotic-like proteins, such as ankyrin repeats, playing important roles in sponge-symbiont interactions, were identified in sponge-associated Leptothoe genomes. The sponge-associated Leptothoe stains harbored biosynthetic gene clusters encoding novel natural products despite genome reduction. Comparisons of the biosynthetic capacities of Leptothoe with chemically rich cyanobacteria revealed that Leptothoe is another promising marine cyanobacterium for the biosynthesis of novel natural products.

ACS Style

Despoina Konstantinou; Rafael Popin; David Fewer; Kaarina Sivonen; Spyros Gkelis. Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated Cyanobacterium Leptothoe. Marine Drugs 2021, 19, 298 .

AMA Style

Despoina Konstantinou, Rafael Popin, David Fewer, Kaarina Sivonen, Spyros Gkelis. Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated Cyanobacterium Leptothoe. Marine Drugs. 2021; 19 (6):298.

Chicago/Turabian Style

Despoina Konstantinou; Rafael Popin; David Fewer; Kaarina Sivonen; Spyros Gkelis. 2021. "Genome Reduction and Secondary Metabolism of the Marine Sponge-Associated Cyanobacterium Leptothoe." Marine Drugs 19, no. 6: 298.

Journal article
Published: 17 December 2020 in International Journal of Molecular Sciences
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far been focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12, and 24 h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. 2,7-dichlorofluorescein diacetate (DCF-DA) staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC−) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences 2020, 21, 9649 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. International Journal of Molecular Sciences. 2020; 21 (24):9649.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." International Journal of Molecular Sciences 21, no. 24: 9649.

Preprint
Published: 26 November 2020
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Microcystins (MCs) are cyanobacterial toxins and potent inhibitors of protein phosphatases 1 (PP1) and 2A (PP2A), which are involved in plant cytoskeleton (microtubules and F-actin) organization. Therefore, studies on the toxicity of cyanobacterial products on plant cells have so far being focused on MCs. In this study, we investigated the effects of extracts from 16 (4 MC-producing and 12 non-MC-producing) cyanobacterial strains from several habitats, on various enzymes (PP1, trypsin, elastase), on the plant cytoskeleton and H2O2 levels in Oryza sativa (rice) root cells. Seedling roots were treated for various time periods (1, 12 and 24h) with aqueous cyanobacterial extracts and underwent either immunostaining for α-tubulin or staining of F-actin with fluorescent phalloidin. DCF-DA staining was performed for H2O2 imaging. The enzyme assays confirmed the bioactivity of the extracts of not only MC-rich (MC+), but also MC-devoid (MC-) extracts, which induced major time-dependent alterations on both components of the plant cytoskeleton. These findings suggest that a broad spectrum of bioactive cyanobacterial compounds, apart from MCs or other known cyanotoxins (such as cylindrospermopsin), can affect plants by disrupting the cytoskeleton.

ACS Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. 2020, 1 .

AMA Style

Dimitris Pappas, Manthos Panou, Ioannis-Dimosthenis S. Adamakis, Spyros Gkelis, Emmanuel Panteris. Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells. . 2020; ():1.

Chicago/Turabian Style

Dimitris Pappas; Manthos Panou; Ioannis-Dimosthenis S. Adamakis; Spyros Gkelis; Emmanuel Panteris. 2020. "Beyond Microcystins: Cyanobacterial Extracts Induce Cytoskeletal Alterations in Rice Root Cells." , no. : 1.

Journal article
Published: 22 October 2020 in Molecular Phylogenetics and Evolution
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Cyanobacteria are often reported as abundant components of the sponge microbiome; however their diversity below the phylum level is still underestimated. Aiming to broaden our knowledge of sponge-cyanobacteria association, we isolated cyanobacterial strains from Aegean Sea sponges in previous research, which revealed high degree of novel cyanobacterial diversity. Herein, we aim to further characterize sponge-associated cyanobacteria and re-evaluate their classification based on an extensive polyphasic approach, i.e. a combination of molecular, morphological and ecological data. This approach resulted in the description of five new genera (Rhodoploca, Cymatolege, Metis, Aegeococcus, and Thalassoporum) and seven new species (R. sivonenia, C. spiroidea, C. isodiametrica, M. fasciculata, A. anagnostidisi, A. thureti, T. komareki) inside the order Synechococcales, and a new pleurocapsalean species (Xenococcus spongiosum). X. spongiosum is a baeocyte-producing species that shares some morphological features with other Xenococcus species, but has distinct phylogenetic and ecological identity. Rhodoploca, Cymatolege, Metis and Thalassoporum are novel well supported linages of filamentous cyanobacteria that possess distinct characters compared to their sister taxa. Aegeococcus is a novel monophyletic linage of Synechococcus-like cyanobacteria exhibiting a unique ecology, as sponge-dweller. The considerable number of novel taxa characterized in this study highlights the importance of employing polyphasic culture-dependent approaches in order to reveal the true cyanobacterial diversity associated with sponges.

ACS Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Spyros Gkelis. Revealing new sponge-associated cyanobacterial diversity: Novel genera and species. Molecular Phylogenetics and Evolution 2020, 155, 106991 .

AMA Style

Despoina Konstantinou, Eleni Voultsiadou, Emmanuel Panteris, Spyros Gkelis. Revealing new sponge-associated cyanobacterial diversity: Novel genera and species. Molecular Phylogenetics and Evolution. 2020; 155 ():106991.

Chicago/Turabian Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Spyros Gkelis. 2020. "Revealing new sponge-associated cyanobacterial diversity: Novel genera and species." Molecular Phylogenetics and Evolution 155, no. : 106991.

Original article
Published: 18 July 2020 in Journal of Eukaryotic Microbiology
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Symbioses between sponges and photosynthetic organisms are very diverse regarding the taxonomy and biogeography of both hosts and symbionts; to date, most research has focused on the exploration of bacterial diversity. The present study aims to characterize the culturable diversity of photosynthetic eukaryotes associated with sponges in the Aegean Sea, on which no information exists. Five microalgae strains were isolated from marine sponges; the strains were characterized by morphological features, and the 18S rRNA, 18S‐28S Internal Transcribed Spacer, and ribulose‐bisphosphate carboxylase large chain (rbc L) sequences. Our polyphasic approach showed that the strains belonged to the green‐alga Acrochaete leptochaete , the diatom Nanofrustulum cf. shiloi , the rhodophyte Acrochaetium spongicola , and the chlorachniophyte Lotharella oceanica . A. leptochaete is reported for the first time in sponges, even though green algae are known to be associated with sponges. Nanofrustulum shiloi was found in association with the sponges Agelas oroides and Chondrilla nucula , whereas information existed only for its association with the species Aplysina aerophoba . Acrochaetium spongicola was found for the first time in association with sponges in the eastern Mediterranean. Moreover, we report herein for the first time a sponge‐chlorarachniophycean association. Our research revealed new diversity of microalgae associated with sponges and added new records of sponge species, previously unknown for their association with microalgae.

ACS Style

Despoina Konstantinou; Rafaela V. Kakakiou; Emmanuel Panteris; Eleni Voultsiadou; Spyros Gkelis. Photosynthetic Sponge‐associated Eukaryotes in the Aegean Sea: A Culture‐dependent Approach. Journal of Eukaryotic Microbiology 2020, 67, 1 .

AMA Style

Despoina Konstantinou, Rafaela V. Kakakiou, Emmanuel Panteris, Eleni Voultsiadou, Spyros Gkelis. Photosynthetic Sponge‐associated Eukaryotes in the Aegean Sea: A Culture‐dependent Approach. Journal of Eukaryotic Microbiology. 2020; 67 (6):1.

Chicago/Turabian Style

Despoina Konstantinou; Rafaela V. Kakakiou; Emmanuel Panteris; Eleni Voultsiadou; Spyros Gkelis. 2020. "Photosynthetic Sponge‐associated Eukaryotes in the Aegean Sea: A Culture‐dependent Approach." Journal of Eukaryotic Microbiology 67, no. 6: 1.

Journal article
Published: 17 July 2020 in Toxicon
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The production of β-Ν-methylamino-L-alanine (BMAA) in cyanobacteria is triggered by nitrogen-starvation conditions and its biological role, albeit unknown, is associated with nitrogen assimilation. In the present study, the effect of BMAA (773 μg L−1) on nitrogen metabolism and physiology of the non-diazotrophic cyanobacterium and non-BMAA producer, Synechococcus sp. TAU-MAC 0499, was investigated. In order to study the combined effect of nitrogen availability and BMAA, nitrogen-starvation conditions were induced by transferring cells in nitrogen-free medium and subsequently exposing the cultures to BMAA. After short-term treatment (180 min) and in the presence of nitrogen, BMAA inhibited glutamine synthetase, which resulted in low concentration of glutamine. In the absence of nitrogen, although there was no effect on glutamine synthetase, a possible perturbation in nitrogen assimilation is reflected on the significant decrease in glutamate levels. During the long-term exposure (24–96 h), growth, photosynthetic pigments and total protein were not affected by BMAA exposure, except for an increase in protein and phycocyanin levels at 48 h in nitrogen replete conditions. Results suggest that BMAA interferes with nitrogen assimilation, in a different way, depending on the presence or absence of combined nitrogen, providing novel data on the potential biological role of BMAA.

ACS Style

Yolanda Vergou; Maria Touraki; Aikaterina Paraskevopoulou; Theodoros M. Triantis; Anastasia Hiskia; Spyros Gkelis. β-Ν-Methylamino-L-alanine interferes with nitrogen assimilation in the cyanobacterium, non-BMAA producer, Synechococcus sp. TAU-MAC 0499. Toxicon 2020, 185, 147 -155.

AMA Style

Yolanda Vergou, Maria Touraki, Aikaterina Paraskevopoulou, Theodoros M. Triantis, Anastasia Hiskia, Spyros Gkelis. β-Ν-Methylamino-L-alanine interferes with nitrogen assimilation in the cyanobacterium, non-BMAA producer, Synechococcus sp. TAU-MAC 0499. Toxicon. 2020; 185 ():147-155.

Chicago/Turabian Style

Yolanda Vergou; Maria Touraki; Aikaterina Paraskevopoulou; Theodoros M. Triantis; Anastasia Hiskia; Spyros Gkelis. 2020. "β-Ν-Methylamino-L-alanine interferes with nitrogen assimilation in the cyanobacterium, non-BMAA producer, Synechococcus sp. TAU-MAC 0499." Toxicon 185, no. : 147-155.

Research paper
Published: 08 April 2020 in Plant Biology
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Microcystins are toxins produced by cyanobacteria, notorious for negatively affecting a wide range of living organisms, among which several plant species. Although microtubules are a well‐established target of microcystin toxicity, its effect on filamentous actin (F‐actin) in plant cells has not been studied yet. Τhe effects of microcystin‐LR (MC‐LR) and the extract of a microcystin‐producing freshwater cyanobacterial strain (Microcystis flos‐aquae TAU‐MAC 1510) on the cytoskeleton (F‐actin and microtubules) of Oryza sativa (rice) root cells, were studied by light, confocal, and transmission electron microscopy. Considering the role of F‐actin in endomembrane system distribution, the endoplasmic reticulum and the Golgi apparatus in extract‐treated cells were also examined. F‐actin in both MC‐LR‐ and extract‐treated meristematic and differentiating root cells exhibited time‐dependent alterations, ranging from disorientation and bundling to the formation of ring‐like structures, eventually resulting to a collapse of the F‐actin network at longer treatments. Disorganization and eventual depolymerization of microtubules, as well as abnormal chromatin condensation were observed following treatment with the extract, effects which could be attributed to microcystins and other bioactive compounds. Moreover, cell cycle progression was inhibited in extract‐treated roots, specifically affecting the mitotic events. As a consequence of F‐actin network disorganization, endoplasmic reticulum elements appeared stacked and diminished, while Golgi dictyosomes appeared aggregated. These results support that F‐actin is a prominent target of MC‐LR, both in pure form and as an extract ingredient. Endomembrane system alterations can also be attributed to the effects of cyanobacterial bioactive compounds (including microcystins) on F‐actin cytoskeleton.

ACS Style

D. Pappas; S. Gkelis; E. Panteris. The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity. Plant Biology 2020, 22, 839 -849.

AMA Style

D. Pappas, S. Gkelis, E. Panteris. The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity. Plant Biology. 2020; 22 (5):839-849.

Chicago/Turabian Style

D. Pappas; S. Gkelis; E. Panteris. 2020. "The effects of microcystin‐LR in Oryza sativa root cells: F‐actin as a new target of cyanobacterial toxicity." Plant Biology 22, no. 5: 839-849.

Preprint content
Published: 18 February 2020
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Microcystins are toxins produced by cyanobacteria, notorious for negatively affecting a wide range of living organisms, among which several plant species. Although microtubules are a well-established target of microcystin toxicity, its effect on filamentous actin (F-actin) in plant cells has not been studied yet.The effects of microcystin-LR (MC-LR) and the extract of a microcystin-producing freshwater cyanobacterial strain (Microcystis flos-aquae TAU-MAC 1510) on the cytoskeleton (F-actin and microtubules) of Oryza sativa (rice) root cells, were studied by light, confocal, and transmission electron microscopy. Considering the role of F-actin in endomembrane system distribution, the endoplasmic reticulum and the Golgi apparatus in extract-treated cells were also examined.F-actin in both MC-LR- and extract-treated meristematic and differentiating root cells exhibited time-dependent alterations, ranging from disorientation and bundling to the formation of ring-like structures, eventually resulting to a collapse of the F-actin network at longer treatments. Disorganization and eventual depolymerization of microtubules, as well as abnormal chromatin condensation were observed following treatment with the extract, effects which could be attributed to microcystins and other bioactive compounds. Moreover, cell cycle progression was inhibited in extract-treated roots, specifically affecting the mitotic events. As a consequence of F-actin network disorganization, endoplasmic reticulum elements appeared stacked and diminished, while Golgi dictyosomes appeared aggregated.These results support that F-actin is a prominent target of MC-LR, both in pure form and as an extract ingredient. Endomembrane system alterations can also be attributed to the effects of cyanobacterial bioactive compounds (including microcystins) on F-actin cytoskeleton.

ACS Style

Dimitris Pappas; Spyros Gkelis; Emmanuel Panteris. The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity. 2020, 1 .

AMA Style

Dimitris Pappas, Spyros Gkelis, Emmanuel Panteris. The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity. . 2020; ():1.

Chicago/Turabian Style

Dimitris Pappas; Spyros Gkelis; Emmanuel Panteris. 2020. "The effects of microcystin-LR in Oryza sativa root cells: F-actin as a new target of cyanobacterial toxicity." , no. : 1.

Journal article
Published: 23 January 2020 in Toxins
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Marine cyanobacteria are considered a prolific source of bioactive natural products with a range of biotechnological and pharmacological applications. However, data on the production of natural compounds from sponge-associated cyanobacteria are scarce. This study aimed to assess the potential of sponge-associated cyanobacteria strains representing different taxonomic groups for the production of bioactive compounds and the biological activity of their extracts. Phylogenetic analysis of sponge-associated cyanobacteria and screening for the presence of genes encoding non-ribosomal peptide synthetases (NRPSs) and polyketide synthases (PKSs) were performed. Methanol extracts of the sponge-associated strains were analyzed for cyanotoxin production and tested for antioxidant activity and cytotoxic activity against several human cancer cell lines and pathogenic bacteria. PKS were detected in all sponge-associated strains examined, indicating the metabolic potential of the isolates. PKS genes were more ubiquitous than NRPS genes. Cyanotoxins (i.e., cylindrospermopsin, anatoxin-a, nodularin, and microcystins) were not detected in any of the sponge-associated cyanobacterial strains. Strains belonging to Leptothoe, Pseudanabaena, and Synechococcus were found to have activity mainly against Staphylococcus aureus. In addition, sponge-associated Leptothoe strains (TAU-MAC 0915, 1015, 1115, and 1215) were found to be highly cytotoxic and in most cases more effective against human cancer cell lines than against normal cells. Extracts with the most promising bioactivity deserve further investigation in order to isolate and identify the bioactive molecule(s).

ACS Style

Despoina Konstantinou; Eleni Mavrogonatou; Sevasti-Kiriaki Zervou; Panagiotis Giannogonas; Spyros Gkelis. Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds. Toxins 2020, 12, 73 .

AMA Style

Despoina Konstantinou, Eleni Mavrogonatou, Sevasti-Kiriaki Zervou, Panagiotis Giannogonas, Spyros Gkelis. Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds. Toxins. 2020; 12 (2):73.

Chicago/Turabian Style

Despoina Konstantinou; Eleni Mavrogonatou; Sevasti-Kiriaki Zervou; Panagiotis Giannogonas; Spyros Gkelis. 2020. "Bioprospecting Sponge-Associated Marine Cyanobacteria to Produce Bioactive Compounds." Toxins 12, no. 2: 73.

Journal article
Published: 20 January 2020 in Chemosphere
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Cyanobacteria can form extensive blooms in water with concurrent production and release of a large number of chemically diverse and bioactive metabolites, including hazardous toxins. Significant number of the metabolites belongs to non-ribosomal peptides, with unique residues, unusual structures and great potential for biotechnological application. The biosynthetic pathways of the peptides generate tens of variants, but only part of them has been identified. Microginins are an understudied class of cyanobacterial linear peptides with a characteristic decanoic acid derivative amino acid residue in their structure. In this study, cyanobacterial blooms and isolated strains from Greek lakes were analyzed for the presence of microginins by liquid chromatography coupled to hybrid triple quadrupole/linear ion trap mass spectrometer (LC-qTRAP MS/MS). Microginin structures were elucidated based on the obtained fragmentation spectra. A large number of microginins occurred in blooms of Greek freshwaters and the most frequently detected were Microginin FR1 (70% of samples), Microginin T1 (52%), Microginin 565B (52%), Microginin T2 (43%), and Microginin 565A (43%). Additionally, nine cyanobacterial strains i.e. Nostoc oryzae, Synechococcus sp., Microcystis aeruginosa, Microcystis viridis, and five Microcystis sp., were found to produce microginins. Thirty-six new microginin structures were characterized out of fifty-one totally detected variants. This is the first time that such a diversity of microginins is reported to be present in water bodies. Results clearly demonstrate the great metabolomic potential of cyanobacteria that inhabit Greek freshwaters and significantly expand the knowledge of cyanobacterial secondary metabolites with regards to the class of microginins.

ACS Style

Sevasti - Kiriaki Zervou; Spyros Gkelis; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. New microginins from cyanobacteria of Greek freshwaters. Chemosphere 2020, 248, 125961 .

AMA Style

Sevasti - Kiriaki Zervou, Spyros Gkelis, Triantafyllos Kaloudis, Anastasia Hiskia, Hanna Mazur-Marzec. New microginins from cyanobacteria of Greek freshwaters. Chemosphere. 2020; 248 ():125961.

Chicago/Turabian Style

Sevasti - Kiriaki Zervou; Spyros Gkelis; Triantafyllos Kaloudis; Anastasia Hiskia; Hanna Mazur-Marzec. 2020. "New microginins from cyanobacteria of Greek freshwaters." Chemosphere 248, no. : 125961.

Preprint content
Published: 06 January 2020
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Cyanobacteria have been linked with hydrogen cyanide, based on their ability to catabolize it by the nitrogenase enzyme, as a part of nitrogen fixation. Nitrogenase can also use hydrogen cyanide instead of its normal substrate, dinitrogen and convert it to methane and ammonia. In this study, we tested whether cyanobacteria are able, not only to reduce, but also to produce HCN. The production of HCN was examined in 78 cyanobacteria strains from all five principal sections of cyanobacteria, both non-heterocytous and heterocytous, representing a variety of lifestyles and habitats. Twenty-eight (28) strains were found positive for HCN production, with universal representation amongst 22 cyanobacterial planktic and epilithic genera inhabiting freshwater, brackish, marine (including sponges), and terrestrial (including anchialine) habitats. The HCN production could be linked with nitrogen fixation, as all of HCN producing strains are considered capable of fixing nitrogen. Epilithic lifestyle, where cyanobacteria are more vulnerable to a number of grazers and accumulate more glycine, had the largest percentage (75%) of HCN-producing cyanobacteria compared to strains from aquatic ecosystems. Further, we demonstrate the isolation and characterisation of taxa likeGeitleria calcareaandKovacikia muscicola, for which no strain existed andChlorogloeasp. TAU-MAC 0618 which is, to the best of our knowledge, the first bacterium isolate from anchialine ecosystems. Our results highlight the complexity of cyanobacteria secondary metabolism, as well as the diversity of cyanobacteria in underexplored habitats, providing a missing study material for this type of environments.

ACS Style

Manthos Panou; Spyros Gkelis. Cyano-assassins: Widespread cyanogenic production from cyanobacteria. 2020, 1 .

AMA Style

Manthos Panou, Spyros Gkelis. Cyano-assassins: Widespread cyanogenic production from cyanobacteria. . 2020; ():1.

Chicago/Turabian Style

Manthos Panou; Spyros Gkelis. 2020. "Cyano-assassins: Widespread cyanogenic production from cyanobacteria." , no. : 1.

Journal article
Published: 30 October 2019 in Journal of Biological Research-Thessaloniki
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Background Terrestrial, freshwater and marine green algae constitute the large and morphologically diverse phylum of Chlorophyta, which gave rise to the core chlorophytes. Chlorophyta are abundant and diverse in freshwater environments where sometimes they form nuisance blooms under eutrophication conditions. The phylogenetic relationships among core chlorophyte clades (Chlorodendrophyceae, Ulvophyceae, Trebouxiophyceae and Chlorophyceae), are of particular interest as it is a species-rich phylum with ecological importance worldwide, but are still poorly understood. In the Mediterranean ecoregion, data on molecular characterization of eukaryotic microalgae strains are limited and current knowledge is based on ecological studies of natural populations. In the present study we report the isolation and characterization of 11 green microalgae strains from Greece contributing more information for the taxonomy of Chlorophyta. The study combined morphological and molecular data. Results Phylogenetic analysis based on 18S rRNA, internal transcribed spacer (ITS) region and the large subunit of the ribulose-bisphosphate carboxylase (rbcL) gene revealed eight taxa. Eleven green algae strains were classified in four orders (Sphaeropleales, Chlorellales, Chlamydomonadales and Chaetophorales) and were represented by four genera; one strain was not assigned to any genus. Most strains (six) were classified to the genus Desmodesmus, two strains to genus Chlorella, one to genus Spongiosarcinopsis and one filamentous strain to genus Uronema. One strain is placed in a separate independent branch within the Chlamydomonadales and deserves further research. Conclusions Our study reports, for the first time, the presence of Uronema in an aquatic environment up to 40 °C and reveals new diversity within the Chlamydomonadales. The results from the ITS region and the rbcL gene corroborated those obtained from 18S rRNA without providing further information or resolving the phylogenetic relationships within certain genera, due to the limited number of ITS and rbcL sequences available. The comparison of molecular and morphological data showed that they were congruent. Cosmopolitan genera with high worldwide distribution inhabit Greek freshwaters.

ACS Style

Urania Lortou; Spyros Gkelis. Polyphasic taxonomy of green algae strains isolated from Mediterranean freshwaters. Journal of Biological Research-Thessaloniki 2019, 26, 1 -12.

AMA Style

Urania Lortou, Spyros Gkelis. Polyphasic taxonomy of green algae strains isolated from Mediterranean freshwaters. Journal of Biological Research-Thessaloniki. 2019; 26 (1):1-12.

Chicago/Turabian Style

Urania Lortou; Spyros Gkelis. 2019. "Polyphasic taxonomy of green algae strains isolated from Mediterranean freshwaters." Journal of Biological Research-Thessaloniki 26, no. 1: 1-12.

Journal article
Published: 25 July 2019 in Toxins
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Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization and morphological features, except for the Jaaginema–Limnothrix group which appears intermixed and needs further elucidation. Methanol extracts of the strains were analyzed for cyanotoxin production and tested against pathogenic bacteria species and several cancer cell lines. We report for the first time a Nostoc oryzae strain isolated from rice fields capable of producing microcystins (MCs) and a Chlorogloeopsis fritschii strain isolated from the plankton of a lake, suggesting that this species may also occur in freshwater temperate habitats. Strains with very high or identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activities. Extracts from Synechococcus cf. nidulans showed the most potent antibacterial activity against Staphylococcus aureus, whereas Jaaginema sp. strains exhibited potent cytotoxic activities against human colorectal adenocarcinoma and hepatocellular carcinoma cells. Jaaginema Thessaloniki Aristotle University Microalgae and Cyanobacteria (TAU-MAC) 0110 and 0210 strains caused pronounced changes in the actin network and triggered the formation of numerous lipid droplets in hepatocellular carcinoma and green monkey kidney cells, suggesting oxidative stress and/or mitochondrial damage leading to apoptosis.

ACS Style

Spyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins 2019, 11, 436 .

AMA Style

Spyros Gkelis, Manthos Panou, Despoina Konstantinou, Panagiotis Apostolidis, Antonia Kasampali, Sofia Papadimitriou, Dominiki Kati, Giorgia Maria Di Lorenzo, Stamatia Ioakeim, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Minas Arsenakis. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins. 2019; 11 (8):436.

Chicago/Turabian Style

Spyros Gkelis; Manthos Panou; Despoina Konstantinou; Panagiotis Apostolidis; Antonia Kasampali; Sofia Papadimitriou; Dominiki Kati; Giorgia Maria Di Lorenzo; Stamatia Ioakeim; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Minas Arsenakis. 2019. "Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece." Toxins 11, no. 8: 436.

Journal article
Published: 11 July 2019 in European Journal of Phycology
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ACS Style

Georgia Papapanagiotou; Spyros Gkelis. Taxonomic revision of commercially used Arthrospira (Cyanobacteria) strains: a polyphasic approach. European Journal of Phycology 2019, 54, 595 -608.

AMA Style

Georgia Papapanagiotou, Spyros Gkelis. Taxonomic revision of commercially used Arthrospira (Cyanobacteria) strains: a polyphasic approach. European Journal of Phycology. 2019; 54 (4):595-608.

Chicago/Turabian Style

Georgia Papapanagiotou; Spyros Gkelis. 2019. "Taxonomic revision of commercially used Arthrospira (Cyanobacteria) strains: a polyphasic approach." European Journal of Phycology 54, no. 4: 595-608.

Regular article
Published: 18 April 2019 in Journal of Phycology
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Cyanobacterial diversity associated with sponges remains underestimated, though it is of great scientific interest in order to understand the ecology and evolutionary history of the symbiotic relationships between the two groups. Of the filamentous cyanobacteria, the genus Leptolyngbya is the most frequently found in association with sponges as well as the largest and obviously polyphyletic group. In this study, five Leptolyngbya‐like sponge‐associated isolates were investigated using a combination of molecular, chemical, and morphological approach and revealed a novel marine genus herein designated Leptothoe gen. nov. In addition, three new species of Leptothoe, Le. sithoniana, Le. kymatousa and Le. spongobia, are described based on a suite of distinct characters compared to other marine Leptolyngbyaceae species/strains. The three new species, hosted by four sponge species, showed different degrees of host specificity. Leptothoe sithoniana and Le. kymatousa hosted by the sponges Petrosia ficiformis and Chondrilla nucula respectively, seem to be more specialized than Le. spongobia, which was hosted by the sponges Dysidea avara and Acanthella acuta. All three species contained nitrogen‐fixing genes and may contribute to the nitrogen budget of sponges. Leptothoe spongobia TAU‐MAC 1115 isolated from Acanthella acuta, was shown to produce microcystin‐RR, indicating that microcystin production among marine cyanobacteria could be more widespread than previously determined.

ACS Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Sevasti‐Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis. Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology 2019, 55, 882 -897.

AMA Style

Despoina Konstantinou, Eleni Voultsiadou, Emmanuel Panteris, Sevasti‐Kiriaki Zervou, Anastasia Hiskia, Spyros Gkelis. Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea. Journal of Phycology. 2019; 55 (4):882-897.

Chicago/Turabian Style

Despoina Konstantinou; Eleni Voultsiadou; Emmanuel Panteris; Sevasti‐Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis. 2019. "Leptothoe, a new genus of marine cyanobacteria (Synechococcales) and three new species associated with sponges from the Aegean Sea." Journal of Phycology 55, no. 4: 882-897.

Data descriptor
Published: 23 October 2018 in Scientific Data
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Under ongoing climate change and increasing anthropogenic activity, which continuously challenge ecosystem resilience, an in-depth understanding of ecological processes is urgently needed. Lakes, as providers of numerous ecosystem services, face multiple stressors that threaten their functioning. Harmful cyanobacterial blooms are a persistent problem resulting from nutrient pollution and climate-change induced stressors, like poor transparency, increased water temperature and enhanced stratification. Consistency in data collection and analysis methods is necessary to achieve fully comparable datasets and for statistical validity, avoiding issues linked to disparate data sources. The European Multi Lake Survey (EMLS) in summer 2015 was an initiative among scientists from 27 countries to collect and analyse lake physical, chemical and biological variables in a fully standardized manner. This database includes in-situ lake variables along with nutrient, pigment and cyanotoxin data of 369 lakes in Europe, which were centrally analysed in dedicated laboratories. Publishing the EMLS methods and dataset might inspire similar initiatives to study across large geographic areas that will contribute to better understanding lake responses in a changing environment. Machine-accessible metadata file describing the reported data (ISA-Tab format)

ACS Style

Evanthia Mantzouki; James Campbell; Emiel van Loon; Petra Visser; Iosif Konstantinou; Maria Antoniou; Gregory Giuliani; Danielle Machado-Vieira; Alinne Gurjão de Oliveira; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Itana Bokan Vucelić; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Nikoletta Tsiarta; Luděk Bláha; Rodan Geriš; Markéta Fránková; Kirsten Seestern Christoffersen; Trine Perlt Warming; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Kersti Kangro; Kerstin Häggqvist; Pauliina Salmi; Lauri Arvola; Jutta Fastner; Dietmar Straile; Karl-Otto Rothhaupt; Jeremy Fonvielle; Hans-Peter Grossart; Christos Avagianos; Triantafyllos Kaloudis; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis; Manthos Panou; Valerie McCarthy; Victor C. Perello; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Judita Koreivienė; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Sigrid Haande; Birger Skjelbred; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Lidia Nawrocka; Justyna Kobos; Hanna Mazur-Marzec; Pablo Alcaraz-Párraga; Elzbieta Wilk-Wozniak; Wojciech Krztoń; Edward Walusiak; Ilona Gagala; Joanna Mankiewicz-Boczek; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Agnieszka Napiórkowska-Krzebietke; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Beata Madrecka; Iwona Kostrzewska-Szlakowska; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Agnieszka Ochocka; Agnieszka Pasztaleniec; Iwona Jasser; Ana M. Antão-Geraldes; Manel Leira; Armand Hernández; Vitor Vasconcelos; João Morais; Micaela Vale; Pedro M. Raposeiro; Vítor Gonçalves; Boris Aleksovski; Svetislav Krstić; Hana Nemova; Iveta Drastichova; Lucia Chomova; Spela Remec-Rekar; Tina Elersek; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Bárbara Úbeda; José Ángel Gálvez; Rafael Marcé; Núria Catalán; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Carmen Cillero-Castro; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Soria; Lars-Anders Hansson; Pablo Urrutia-Cordero; Arda Özen; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Jolanda M. H. Verspagen; Lisette De Senerpont Domis; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Miquel Lürling; Valentini Maliaka; Elisabeth J. Faassen; Delphine Latour; Cayelan C. Carey; Hans W. Paerl; Andrea Torokne; Tünay Karan; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Kemal Çelik; Koray Ozhan; Nusret Karakaya; Mehmet Ali Turan Koçer; Mete Yilmaz; Faruk Maraşlıoğlu; Özden Fakioglu; Elif Neyran Soylu; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Latife Köker; Reyhan Akçaalan; Meriç Albay; Mehmet Tahir Alp; Korhan Özkan; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Jessica Richardson; Christine Edwards; Victoria Bergkemper; Sarah O'Leary; Eilish Beirne; Hannah Cromie; Bastiaan W. Ibelings. A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins. Scientific Data 2018, 5, 1 -13.

AMA Style

Evanthia Mantzouki, James Campbell, Emiel van Loon, Petra Visser, Iosif Konstantinou, Maria Antoniou, Gregory Giuliani, Danielle Machado-Vieira, Alinne Gurjão de Oliveira, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer, Itana Bokan Vucelić, Petar Žutinić, Marija Gligora Udovič, Anđelka Plenković-Moraj, Nikoletta Tsiarta, Luděk Bláha, Rodan Geriš, Markéta Fránková, Kirsten Seestern Christoffersen, Trine Perlt Warming, Tõnu Feldmann, Alo Laas, Kristel Panksep, Lea Tuvikene, Kersti Kangro, Kerstin Häggqvist, Pauliina Salmi, Lauri Arvola, Jutta Fastner, Dietmar Straile, Karl-Otto Rothhaupt, Jeremy Fonvielle, Hans-Peter Grossart, Christos Avagianos, Triantafyllos Kaloudis, Theodoros Triantis, Sevasti-Kiriaki Zervou, Anastasia Hiskia, Spyros Gkelis, Manthos Panou, Valerie McCarthy, Victor C. Perello, Ulrike Obertegger, Adriano Boscaini, Giovanna Flaim, Nico Salmaso, Leonardo Cerasino, Judita Koreivienė, Jūratė Karosienė, Jūratė Kasperovičienė, Ksenija Savadova, Irma Vitonytė, Sigrid Haande, Birger Skjelbred, Magdalena Grabowska, Maciej Karpowicz, Damian Chmura, Lidia Nawrocka, Justyna Kobos, Hanna Mazur-Marzec, Pablo Alcaraz-Párraga, Elzbieta Wilk-Wozniak, Wojciech Krztoń, Edward Walusiak, Ilona Gagala, Joanna Mankiewicz-Boczek, Magdalena Toporowska, Barbara Pawlik-Skowronska, Michał Niedźwiecki, Wojciech Pęczuła, Agnieszka Napiórkowska-Krzebietke, Julita Dunalska, Justyna Sieńska, Daniel Szymański, Marek Kruk, Agnieszka Budzyńska, Ryszard Goldyn, Anna Kozak, Joanna Rosińska, Elżbieta Szeląg-Wasielewska, Piotr Domek, Natalia Jakubowska-Krepska, Kinga Kwasizur, Beata Messyasz, Aleksandra Pełechata, Mariusz Pełechaty, Mikolaj Kokocinski, Beata Madrecka, Iwona Kostrzewska-Szlakowska, Magdalena Frąk, Agnieszka Bańkowska-Sobczak, Michał Wasilewicz, Agnieszka Ochocka, Agnieszka Pasztaleniec, Iwona Jasser, Ana M. Antão-Geraldes, Manel Leira, Armand Hernández, Vitor Vasconcelos, João Morais, Micaela Vale, Pedro M. Raposeiro, Vítor Gonçalves, Boris Aleksovski, Svetislav Krstić, Hana Nemova, Iveta Drastichova, Lucia Chomova, Spela Remec-Rekar, Tina Elersek, Jordi Delgado-Martín, David García, Jose Luís Cereijo, Joan Gomà, Mari Carmen Trapote, Teresa Vegas-Vilarrúbia, Biel Obrador, Ana García-Murcia, Monserrat Real, Elvira Romans, Jordi Noguero-Ribes, David Parreño Duque, Elísabeth Fernández-Morán, Bárbara Úbeda, José Ángel Gálvez, Rafael Marcé, Núria Catalán, Carmen Pérez-Martínez, Eloísa Ramos-Rodríguez, Carmen Cillero-Castro, Enrique Moreno-Ostos, José María Blanco, Valeriano Rodríguez, Jorge Juan Montes-Pérez, Roberto L. Palomino, Estela Rodríguez-Pérez, Rafael Carballeira, Antonio Camacho, Antonio Picazo, Carlos Rochera, Anna C. Santamans, Carmen Ferriol, Susana Romo, Juan Soria, Lars-Anders Hansson, Pablo Urrutia-Cordero, Arda Özen, Andrea G. Bravo, Moritz Buck, William Colom-Montero, Kristiina Mustonen, Don Pierson, Yang Yang, Jolanda M. H. Verspagen, Lisette De Senerpont Domis, Laura Seelen, Sven Teurlincx, Yvon Verstijnen, Miquel Lürling, Valentini Maliaka, Elisabeth J. Faassen, Delphine Latour, Cayelan C. Carey, Hans W. Paerl, Andrea Torokne, Tünay Karan, Nilsun Demir, Meryem Beklioğlu, Nur Filiz, Eti E. Levi, Uğur Iskin, Gizem Bezirci, Ülkü Nihan Tavşanoğlu, Kemal Çelik, Koray Ozhan, Nusret Karakaya, Mehmet Ali Turan Koçer, Mete Yilmaz, Faruk Maraşlıoğlu, Özden Fakioglu, Elif Neyran Soylu, Meral Apaydın Yağcı, Şakir Çınar, Kadir Çapkın, Abdulkadir Yağcı, Mehmet Cesur, Fuat Bilgin, Cafer Bulut, Rahmi Uysal, Latife Köker, Reyhan Akçaalan, Meriç Albay, Mehmet Tahir Alp, Korhan Özkan, Tuğba Ongun Sevindik, Hatice Tunca, Burçin Önem, Jessica Richardson, Christine Edwards, Victoria Bergkemper, Sarah O'Leary, Eilish Beirne, Hannah Cromie, Bastiaan W. Ibelings. A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins. Scientific Data. 2018; 5 (1):1-13.

Chicago/Turabian Style

Evanthia Mantzouki; James Campbell; Emiel van Loon; Petra Visser; Iosif Konstantinou; Maria Antoniou; Gregory Giuliani; Danielle Machado-Vieira; Alinne Gurjão de Oliveira; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Itana Bokan Vucelić; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Nikoletta Tsiarta; Luděk Bláha; Rodan Geriš; Markéta Fránková; Kirsten Seestern Christoffersen; Trine Perlt Warming; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Kersti Kangro; Kerstin Häggqvist; Pauliina Salmi; Lauri Arvola; Jutta Fastner; Dietmar Straile; Karl-Otto Rothhaupt; Jeremy Fonvielle; Hans-Peter Grossart; Christos Avagianos; Triantafyllos Kaloudis; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Spyros Gkelis; Manthos Panou; Valerie McCarthy; Victor C. Perello; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Judita Koreivienė; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Sigrid Haande; Birger Skjelbred; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Lidia Nawrocka; Justyna Kobos; Hanna Mazur-Marzec; Pablo Alcaraz-Párraga; Elzbieta Wilk-Wozniak; Wojciech Krztoń; Edward Walusiak; Ilona Gagala; Joanna Mankiewicz-Boczek; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Agnieszka Napiórkowska-Krzebietke; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Beata Madrecka; Iwona Kostrzewska-Szlakowska; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Agnieszka Ochocka; Agnieszka Pasztaleniec; Iwona Jasser; Ana M. Antão-Geraldes; Manel Leira; Armand Hernández; Vitor Vasconcelos; João Morais; Micaela Vale; Pedro M. Raposeiro; Vítor Gonçalves; Boris Aleksovski; Svetislav Krstić; Hana Nemova; Iveta Drastichova; Lucia Chomova; Spela Remec-Rekar; Tina Elersek; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Bárbara Úbeda; José Ángel Gálvez; Rafael Marcé; Núria Catalán; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Carmen Cillero-Castro; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Soria; Lars-Anders Hansson; Pablo Urrutia-Cordero; Arda Özen; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Jolanda M. H. Verspagen; Lisette De Senerpont Domis; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Miquel Lürling; Valentini Maliaka; Elisabeth J. Faassen; Delphine Latour; Cayelan C. Carey; Hans W. Paerl; Andrea Torokne; Tünay Karan; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Kemal Çelik; Koray Ozhan; Nusret Karakaya; Mehmet Ali Turan Koçer; Mete Yilmaz; Faruk Maraşlıoğlu; Özden Fakioglu; Elif Neyran Soylu; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Latife Köker; Reyhan Akçaalan; Meriç Albay; Mehmet Tahir Alp; Korhan Özkan; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Jessica Richardson; Christine Edwards; Victoria Bergkemper; Sarah O'Leary; Eilish Beirne; Hannah Cromie; Bastiaan W. Ibelings. 2018. "A European Multi Lake Survey dataset of environmental variables, phytoplankton pigments and cyanotoxins." Scientific Data 5, no. 1: 1-13.

Journal article
Published: 15 October 2018 in Harmful Algae
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The cyanobacterium Cylindrospermopsis raciborskii represents a challenge for researchers and it is extensively studied for its toxicity and invasive behaviour, which is presumably enhanced by global warming. Biogeography studies indicate a tropical origin for this species, with Greece considered as the expansion route of C. raciborskii in Europe. The widening of its geographic distribution and the isolation of strains showing high optimum growth temperature underline its ecological heterogeneity, suggesting the existence of different ecotypes. The dominance of species like C. raciborskii along with their ecotoxicology and potential human risk related problems, render the establishment of a clear phylogeography model essential. In the context of the present study, the characterization of Cylindrospermopsis raciborskii TAU-MAC 1414 strain, isolated from Lake Karla, with respect to its phylogeography and toxic potential, is attempted. Our research provides new insights on the origin of C. raciborskii in the Mediterranean region; C. raciborskii expanded in Mediterranean from North America, whilst the rest of the European strains may originate from Asia and Australia. Microcystin synthetase genes, phylogenetic closely related with Microcystis strains, were also present in C. raciborskii TAU-MAC 1414. We were unable to unambiguously confirm the presence of MC-LR, using LC–MS/MS. Our results are shedding light on the expansion and distribution of C. raciborskii, whilst they pose further questions on the toxic capacity of this species.

ACS Style

Manthos Panou; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Spyros Gkelis. A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale. Harmful Algae 2018, 80, 96 -106.

AMA Style

Manthos Panou, Sevasti-Kiriaki Zervou, Triantafyllos Kaloudis, Anastasia Hiskia, Spyros Gkelis. A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale. Harmful Algae. 2018; 80 ():96-106.

Chicago/Turabian Style

Manthos Panou; Sevasti-Kiriaki Zervou; Triantafyllos Kaloudis; Anastasia Hiskia; Spyros Gkelis. 2018. "A Greek Cylindrospermopsis raciborskii strain: Missing link in tropic invader’s phylogeography tale." Harmful Algae 80, no. : 96-106.

Journal article
Published: 13 April 2018 in Toxins
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Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.

ACS Style

Evanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins 2018, 10, 156 .

AMA Style

Evanthia Mantzouki, Miquel Lürling, Jutta Fastner, Lisette De Senerpont Domis, Elżbieta Wilk-Woźniak, Judita Koreivienė, Laura Seelen, Sven Teurlincx, Yvon Verstijnen, Wojciech Krztoń, Edward Walusiak, Jūratė Karosienė, Jūratė Kasperovičienė, Ksenija Savadova, Irma Vitonytė, Carmen Cillero-Castro, Agnieszka Budzyńska, Ryszard Goldyn, Anna Kozak, Joanna Rosińska, Elżbieta Szeląg-Wasielewska, Piotr Domek, Natalia Jakubowska-Krepska, Kinga Kwasizur, Beata Messyasz, Aleksandra Pełechata, Mariusz Pełechaty, Mikolaj Kokocinski, Ana García-Murcia, Monserrat Real, Elvira Romans, Jordi Noguero-Ribes, David Parreño Duque, Elísabeth Fernández-Morán, Nusret Karakaya, Kerstin Häggqvist, Nilsun Demir, Meryem Beklioğlu, Nur Filiz, Eti E. Levi, Uğur Iskin, Gizem Bezirci, Ülkü Nihan Tavşanoğlu, Koray Özhan, Spyros Gkelis, Manthos Panou, Özden Fakioglu, Christos Avagianos, Triantafyllos Kaloudis, Kemal Çelik, Mete Yilmaz, Rafael Marcé, Nuria Catalán, Andrea G. Bravo, Moritz Buck, William Colom-Montero, Kristiina Mustonen, Don Pierson, Yang Yang, Pedro M. Raposeiro, Vítor Gonçalves, Maria G. Antoniou, Nikoletta Tsiarta, Valerie McCarthy, Victor C. Perello, Tõnu Feldmann, Alo Laas, Kristel Panksep, Lea Tuvikene, Ilona Gagala, Joana Mankiewicz-Boczek, Meral Apaydın Yağcı, Şakir Çınar, Kadir Çapkın, Abdulkadir Yağcı, Mehmet Cesur, Fuat Bilgin, Cafer Bulut, Rahmi Uysal, Ulrike Obertegger, Adriano Boscaini, Giovanna Flaim, Nico Salmaso, Leonardo Cerasino, Jessica Richardson, Petra M. Visser, Jolanda M. H. Verspagen, Tünay Karan, Elif Neyran Soylu, Faruk Maraşlıoğlu, Agnieszka Napiórkowska-Krzebietke, Agnieszka Ochocka, Agnieszka Pasztaleniec, Ana M. Antão-Geraldes, Vitor Vasconcelos, João Morais, Micaela Vale, Latife Köker, Reyhan Akçaalan, Meriç Albay, Dubravka Špoljarić Maronić, Filip Stević, Tanja Žuna Pfeiffer, Jeremy Fonvielle, Dietmar Straile, Karl-Otto Rothhaupt, Lars-Anders Hansson, Pablo Urrutia-Cordero, Luděk Bláha, Rodan Geriš, Markéta Fránková, Mehmet Ali Turan Koçer, Mehmet Tahir Alp, Spela Remec-Rekar, Tina Elersek, Theodoros Triantis, Sevasti-Kiriaki Zervou, Anastasia Hiskia, Sigrid Haande, Birger Skjelbred, Beata Madrecka, Hana Nemova, Iveta Drastichova, Lucia Chomova, Christine Edwards, Tuğba Ongun Sevindik, Hatice Tunca, Burçin Önem, Boris Aleksovski, Svetislav Krstić, Itana Bokan Vucelić, Lidia Nawrocka, Pauliina Salmi, Danielle Machado-Vieira, Alinne Gurjão De Oliveira, Jordi Delgado-Martín, David García, Jose Luís Cereijo, Joan Gomà, Mari Carmen Trapote, Teresa Vegas-Vilarrúbia, Biel Obrador, Magdalena Grabowska, Maciej Karpowicz, Damian Chmura, Bárbara Úbeda, José Ángel Gálvez, Arda Özen, Kirsten Seestern Christoffersen, Trine Perlt Warming, Justyna Kobos, Hanna Mazur-Marzec, Carmen Pérez-Martínez, Eloísa Ramos-Rodríguez, Lauri Arvola, Pablo Alcaraz-Párraga, Magdalena Toporowska, Barbara Pawlik-Skowronska, Michał Niedźwiecki, Wojciech Pęczuła, Manel Leira, Armand Hernández, Enrique Moreno-Ostos, José María Blanco, Valeriano Rodríguez, Jorge Juan Montes-Pérez, Roberto L. Palomino, Estela Rodríguez-Pérez, Rafael Carballeira, Antonio Camacho, Antonio Picazo, Carlos Rochera, Anna C. Santamans, Carmen Ferriol, Susana Romo, Juan Miguel Soria, Julita Dunalska, Justyna Sieńska, Daniel Szymański, Marek Kruk, Iwona Kostrzewska-Szlakowska, Iwona Jasser, Petar Žutinić, Marija Gligora Udovič, Anđelka Plenković-Moraj, Magdalena Frąk, Agnieszka Bańkowska-Sobczak, Michał Wasilewicz, Korhan Özkan, Valentini Maliaka, Kersti Kangro, Hans-Peter Grossart, Hans Paerl, Cayelan C. Carey, Bas W. Ibelings. Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins. Toxins. 2018; 10 (4):156.

Chicago/Turabian Style

Evanthia Mantzouki; Miquel Lürling; Jutta Fastner; Lisette De Senerpont Domis; Elżbieta Wilk-Woźniak; Judita Koreivienė; Laura Seelen; Sven Teurlincx; Yvon Verstijnen; Wojciech Krztoń; Edward Walusiak; Jūratė Karosienė; Jūratė Kasperovičienė; Ksenija Savadova; Irma Vitonytė; Carmen Cillero-Castro; Agnieszka Budzyńska; Ryszard Goldyn; Anna Kozak; Joanna Rosińska; Elżbieta Szeląg-Wasielewska; Piotr Domek; Natalia Jakubowska-Krepska; Kinga Kwasizur; Beata Messyasz; Aleksandra Pełechata; Mariusz Pełechaty; Mikolaj Kokocinski; Ana García-Murcia; Monserrat Real; Elvira Romans; Jordi Noguero-Ribes; David Parreño Duque; Elísabeth Fernández-Morán; Nusret Karakaya; Kerstin Häggqvist; Nilsun Demir; Meryem Beklioğlu; Nur Filiz; Eti E. Levi; Uğur Iskin; Gizem Bezirci; Ülkü Nihan Tavşanoğlu; Koray Özhan; Spyros Gkelis; Manthos Panou; Özden Fakioglu; Christos Avagianos; Triantafyllos Kaloudis; Kemal Çelik; Mete Yilmaz; Rafael Marcé; Nuria Catalán; Andrea G. Bravo; Moritz Buck; William Colom-Montero; Kristiina Mustonen; Don Pierson; Yang Yang; Pedro M. Raposeiro; Vítor Gonçalves; Maria G. Antoniou; Nikoletta Tsiarta; Valerie McCarthy; Victor C. Perello; Tõnu Feldmann; Alo Laas; Kristel Panksep; Lea Tuvikene; Ilona Gagala; Joana Mankiewicz-Boczek; Meral Apaydın Yağcı; Şakir Çınar; Kadir Çapkın; Abdulkadir Yağcı; Mehmet Cesur; Fuat Bilgin; Cafer Bulut; Rahmi Uysal; Ulrike Obertegger; Adriano Boscaini; Giovanna Flaim; Nico Salmaso; Leonardo Cerasino; Jessica Richardson; Petra M. Visser; Jolanda M. H. Verspagen; Tünay Karan; Elif Neyran Soylu; Faruk Maraşlıoğlu; Agnieszka Napiórkowska-Krzebietke; Agnieszka Ochocka; Agnieszka Pasztaleniec; Ana M. Antão-Geraldes; Vitor Vasconcelos; João Morais; Micaela Vale; Latife Köker; Reyhan Akçaalan; Meriç Albay; Dubravka Špoljarić Maronić; Filip Stević; Tanja Žuna Pfeiffer; Jeremy Fonvielle; Dietmar Straile; Karl-Otto Rothhaupt; Lars-Anders Hansson; Pablo Urrutia-Cordero; Luděk Bláha; Rodan Geriš; Markéta Fránková; Mehmet Ali Turan Koçer; Mehmet Tahir Alp; Spela Remec-Rekar; Tina Elersek; Theodoros Triantis; Sevasti-Kiriaki Zervou; Anastasia Hiskia; Sigrid Haande; Birger Skjelbred; Beata Madrecka; Hana Nemova; Iveta Drastichova; Lucia Chomova; Christine Edwards; Tuğba Ongun Sevindik; Hatice Tunca; Burçin Önem; Boris Aleksovski; Svetislav Krstić; Itana Bokan Vucelić; Lidia Nawrocka; Pauliina Salmi; Danielle Machado-Vieira; Alinne Gurjão De Oliveira; Jordi Delgado-Martín; David García; Jose Luís Cereijo; Joan Gomà; Mari Carmen Trapote; Teresa Vegas-Vilarrúbia; Biel Obrador; Magdalena Grabowska; Maciej Karpowicz; Damian Chmura; Bárbara Úbeda; José Ángel Gálvez; Arda Özen; Kirsten Seestern Christoffersen; Trine Perlt Warming; Justyna Kobos; Hanna Mazur-Marzec; Carmen Pérez-Martínez; Eloísa Ramos-Rodríguez; Lauri Arvola; Pablo Alcaraz-Párraga; Magdalena Toporowska; Barbara Pawlik-Skowronska; Michał Niedźwiecki; Wojciech Pęczuła; Manel Leira; Armand Hernández; Enrique Moreno-Ostos; José María Blanco; Valeriano Rodríguez; Jorge Juan Montes-Pérez; Roberto L. Palomino; Estela Rodríguez-Pérez; Rafael Carballeira; Antonio Camacho; Antonio Picazo; Carlos Rochera; Anna C. Santamans; Carmen Ferriol; Susana Romo; Juan Miguel Soria; Julita Dunalska; Justyna Sieńska; Daniel Szymański; Marek Kruk; Iwona Kostrzewska-Szlakowska; Iwona Jasser; Petar Žutinić; Marija Gligora Udovič; Anđelka Plenković-Moraj; Magdalena Frąk; Agnieszka Bańkowska-Sobczak; Michał Wasilewicz; Korhan Özkan; Valentini Maliaka; Kersti Kangro; Hans-Peter Grossart; Hans Paerl; Cayelan C. Carey; Bas W. Ibelings. 2018. "Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins." Toxins 10, no. 4: 156.

Research article
Published: 29 March 2018 in PLOS ONE
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Sponge-cyanobacteria associations have attracted research interest from an ecological, evolutionary and biotechnological perspective. Current knowledge is, in its majority, “hidden” in metagenomics research studying the entire microbial communities of sponges, while knowledge on these associations is totally missing for certain geographic areas. In this study, we (a) investigated the occurrence of cyanobacteria in 18 sponge species, several of which are studied for the first time for their cyanobionts, from a previously unexplored eastern Mediterranean ecoregion, the Aegean Sea, (b) isolated sponge-associated cyanobacteria, and characterized them based on a polyphasic (morphological-morphometric and molecular phylogenetic analysis) approach, and (c) conducted a meta-analysis on the global diversity of sponge species hosting cyanobacteria, as well as the diversity of cyanobacterial symbionts. Our research provided new records for nine sponge species, previously unknown for this association, while the isolated cyanobacteria were found to form novel clades within Synechococcus, Leptolyngbyaceae, Pseudanabaenaceae, and Schizotrichaceae, whose taxonomic status requires further investigation; this is the first report of a Schizotrichaceae cyanobacterium associated with sponges. The extensive evaluation of the literature along with the new data from the Aegean Sea raised the number of sponge species known for hosting cyanobacteria to 320 and showed that the cyanobacterial diversity reported from sponges is yet underestimated.

ACS Style

Despoina Konstantinou; Vasilis Gerovasileiou; Eleni Voultsiadou; Spyros Gkelis. Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean. PLOS ONE 2018, 13, e0195001 .

AMA Style

Despoina Konstantinou, Vasilis Gerovasileiou, Eleni Voultsiadou, Spyros Gkelis. Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean. PLOS ONE. 2018; 13 (3):e0195001.

Chicago/Turabian Style

Despoina Konstantinou; Vasilis Gerovasileiou; Eleni Voultsiadou; Spyros Gkelis. 2018. "Sponges-Cyanobacteria associations: Global diversity overview and new data from the Eastern Mediterranean." PLOS ONE 13, no. 3: e0195001.

Journal article
Published: 03 May 2017 in Advances in Oceanography and Limnology
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Lake Karla (Central Greece) is a unique example - at European scale - of a shallow lake ecosystem that was dried in the 1960s and in 2009 started to be restored. The lake is listed in the network of the Greek protected areas as it is considered a vital aquatic ecosystem, in terms of biodiversity. It has, however, already been adversely affected by both agricultural and industrial land uses in the surrounding area, leading to eutrophication and shifting algal community towards bloom-forming toxic cyanobacterial species. After repeated heavy-blooms, cyanotoxin occurrence and mass fish kills, the local ecosystem management authority has implemented a water quality monitoring program (July 2013 - July 2015) to assess environmental pressures and the response of aquatic biota in the lake. Microscopic, immunological, and molecular techniques combined with physico-chemical parameters, complemented by liquid chromatography tandem mass spectrometry (LC-MS/MS), were used to monitor cyanobacteria blooms and the associated cyanotoxin production from three different sites in Lake Karla and from the adjacent Kalamaki Reservoir. Water quality was also assessed by the structure of benthic invertebrate community on the sediment. Cyanobacteria were the main phytoplankton component, representing more than 70% of the total phytoplankton abundance; dominant taxa belonged to Cylindrospermopsis raciborskii, Limnothrix redekei, Anabaenopsis elenkinii, and Microcystis spp. Euglenophytes (Euglena), diatoms (Nitzschia), and chlorophytes (Scenedesmus) were also important phytoplankton constituents. LC-MS/MS confirmed the co-occurrence of microcystins, cylindrospermopsin, saxitoxin, neo-saxitoxin and anatoxin-a. The occurrence of cyanotoxins in relation to the persistent and dominant cyanobacteria and the impact of cyanobacterial harmful algal blooms on the newly constructed lake along with the land uses and the emergent mitigation measures are discussed.

ACS Style

Spyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology 2017, 8, 1 .

AMA Style

Spyros Gkelis, Manthos Panou, Ioannis Chronis, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Korina Manolidi, Chrysoula Ntislidou, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, Ifigenia Kagalou, Maria Lazaridou. Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake. Advances in Oceanography and Limnology. 2017; 8 (1):1.

Chicago/Turabian Style

Spyros Gkelis; Manthos Panou; Ioannis Chronis; Sevasti-Kiriaki Zervou; Christophoros Christophoridis; Korina Manolidi; Chrysoula Ntislidou; Theodoros M. Triantis; Triantafyllos Kaloudis; Anastasia Hiskia; Ifigenia Kagalou; Maria Lazaridou. 2017. "Monitoring a newly re-born patient: water quality and cyanotoxin occurrence in a reconstructed shallow Mediterranean lake." Advances in Oceanography and Limnology 8, no. 1: 1.