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The demand for alternative sources of clean, sustainable, and renewable energy has been a focus of research around the world for the past few decades. Microbial/enzymatic biofuel cells are one of the popular technologies for generating electricity from organic substrates. Currently, one of the promising fuel options is based on glucose due to its multiple advantages: high energy intensity, environmental friendliness, low cost, etc. The effectiveness of biofuel cells is largely determined by the activity of biocatalytic systems applied to accelerate electrode reactions. For this work with aerobic granular sludge as a basis, a nitrogen-fixing community of microorganisms has been selected. The microorganisms were immobilized on a carbon material (graphite foam, carbon nanotubes). The bioanode was developed from a selected biological material. A membraneless biofuel cell glucose/oxygen, with abiotic metal catalysts and biocatalysts based on a microorganism community and enzymes, has been developed. Using methods of laboratory electrochemical studies and mathematical modeling, the physicochemical phenomena and processes occurring in the cell has been studied. The mathematical model includes equations for the kinetics of electrochemical reactions and the growth of microbiological population, the material balance of the components, and charge balance. The results of calculations of the distribution of component concentrations over the thickness of the active layer and over time are presented. The data obtained from the model calculations correspond to the experimental ones. Optimization for fuel concentration has been carried out.
Violetta Vasilenko; Irina Arkadeva; Vera Bogdanovskaya; George Sudarev; Sergei Kalenov; Marco Vocciante; Eleonora Koltsova. Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling. Energies 2020, 13, 5630 .
AMA StyleVioletta Vasilenko, Irina Arkadeva, Vera Bogdanovskaya, George Sudarev, Sergei Kalenov, Marco Vocciante, Eleonora Koltsova. Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling. Energies. 2020; 13 (21):5630.
Chicago/Turabian StyleVioletta Vasilenko; Irina Arkadeva; Vera Bogdanovskaya; George Sudarev; Sergei Kalenov; Marco Vocciante; Eleonora Koltsova. 2020. "Glucose-Oxygen Biofuel Cell with Biotic and Abiotic Catalysts: Experimental Research and Mathematical Modeling." Energies 13, no. 21: 5630.
Uncontrolled bleeding causes more than 30% of deaths due to injuries worldwide. Modern hemostatic materials based on synthetic and biological macromolecules focus mainly on the time prior to hemostasis, but the prevention of a bacterial infection is also crucial for increasing survival. Silver is known to exhibit potent antimicrobial activity and strong biocidal effects against various types of bacteria. A new method for producing of composite sponges with a pronounced antimicrobial effect based on such biological polymers as alginate and chitosan with the addition of silver nanoparticles has been proposed. The method is based on the combination of cryostructuring, ionotropic gelation and ion substitution methods during the formation of the macroporous structure of the sponge at the gelation stage with subsequent freeze drying. Silver nanoparticles isolated by three fungal cultures were introduced into the sponges in low concentrations. The alginate–chitosan–composite sponges with Ag nanoparticles showed high sorption capacity, microporous structure and increased antimicrobial activity compared to a composite sponge without the inclusion of silver nanoparticles.
Mariia G. Gordienko; Vera V. Palchikova; Sergei V. Kalenov; Evgeniy A. Lebedev; Alexei A. Belov; Natalia V. Menshutina. The alginate–chitosan composite sponges with biogenic Ag nanoparticles produced by combining of cryostructuration, ionotropic gelation and ion replacement methods. International Journal of Polymeric Materials and Polymeric Biomaterials 2020, 1 -11.
AMA StyleMariia G. Gordienko, Vera V. Palchikova, Sergei V. Kalenov, Evgeniy A. Lebedev, Alexei A. Belov, Natalia V. Menshutina. The alginate–chitosan composite sponges with biogenic Ag nanoparticles produced by combining of cryostructuration, ionotropic gelation and ion replacement methods. International Journal of Polymeric Materials and Polymeric Biomaterials. 2020; ():1-11.
Chicago/Turabian StyleMariia G. Gordienko; Vera V. Palchikova; Sergei V. Kalenov; Evgeniy A. Lebedev; Alexei A. Belov; Natalia V. Menshutina. 2020. "The alginate–chitosan composite sponges with biogenic Ag nanoparticles produced by combining of cryostructuration, ionotropic gelation and ion replacement methods." International Journal of Polymeric Materials and Polymeric Biomaterials , no. : 1-11.
The development of antiseptics and medical products (bandaging materials, sponges, etc.) based on silver nanoparticles is an essential task due to the growing resistance of pathogenic microorganisms to medicines long used in clinical practice. Using silver nanoparticles for the same purpose is promising, but the potential hazards and cumulative effects in the application of nanoparticles requires a thorough study of those materials. To evaluate the efficiency of antiseptics and medical products based on silver nanoparticles, it is necessary to conduct an in-depth study of the activity of silver nanoparticles in different forms and immobilized in carriers. The study examines the resistance of bacterial and fungal cultures to silver nanoparticles produced by chemical reduction and microbiological synthesis. The study of resistance was carried out in different growth phases of pathogenic microorganisms and in both liquid and solid media. Chemically and microbiologically synthesized nanoparticles were added in the form of a suspension, as well as encapsulated in chitosan-PVA matrices. It was experimentally discovered that, depending on the medium and form of the silver, the antibacterial effect would significantly differ due to changes in the mechanisms regarding the release of nanoparticles and their activity against the cells of pathogenic and potentially pathogenic microorganisms.
Mariia G. Gordienko; Vera V. Palchikova; Sergei V. Kalenov; Alexey Belov; Veronika N. Lyasnikova; Daniil Y. Poberezhniy; Alina V. Chibisova; Vladimir V. Sorokin; Dmitry A. Skladnev. Antimicrobial activity of silver salt and silver nanoparticles in different forms against microorganisms of different taxonomic groups. Journal of Hazardous Materials 2019, 378, 120754 .
AMA StyleMariia G. Gordienko, Vera V. Palchikova, Sergei V. Kalenov, Alexey Belov, Veronika N. Lyasnikova, Daniil Y. Poberezhniy, Alina V. Chibisova, Vladimir V. Sorokin, Dmitry A. Skladnev. Antimicrobial activity of silver salt and silver nanoparticles in different forms against microorganisms of different taxonomic groups. Journal of Hazardous Materials. 2019; 378 ():120754.
Chicago/Turabian StyleMariia G. Gordienko; Vera V. Palchikova; Sergei V. Kalenov; Alexey Belov; Veronika N. Lyasnikova; Daniil Y. Poberezhniy; Alina V. Chibisova; Vladimir V. Sorokin; Dmitry A. Skladnev. 2019. "Antimicrobial activity of silver salt and silver nanoparticles in different forms against microorganisms of different taxonomic groups." Journal of Hazardous Materials 378, no. : 120754.
Metal nanoparticles synthesized by green methods with the use of microorganisms are currently one of the most closely studied types of nanomaterials. It has accurately been shown that the characteristics of metal nanoparticles generated in the presence of different bacteria vary. For the two isogenic strains of obligate methylotrophic bacteria of the wild type (M. quaylei MTT) and its streptomycin-resistant mutant (M. quaylei SMR), the pleiotropic character of streptomycin resistance mutation in the SMR cells has been revealed. It has been shown that both cultures can generate silver nanoparticles. There is a dramatic difference in the formation of palladium nanoparticles, which are formed only in the presence of cells of the streptomycin-resistant mutant M. quaylei SMR. This study shows that closely related isogenic strains of obligate methylotrophic bacteria can be distinguished by the spectra of biogenic nanoparticles of two noble metals. While palladium nanoparticles are only generated by the cells of the streptomycin-resistant mutant M. quaylei SMR, biogenic silver nanoparticles can be generated from both cultures. Thus, the assessment of the ability of microorganisms to form biogenic nanoparticles of different metals allows the revelation of subtle metabolic differences of even close cultures.
Vladimir Sorokin; Anna B. Pshenichnikova; Sergei V. Kalenov; Nikolay A. Suyasov; Dmitry A. Skladnev. Comparison of the Wild-Type Obligate Methylotrophic Bacterium Methylophilus quaylei and its Isogenic Streptomycin-Resistant Mutant via Metal Nanoparticle Generation. Biological Trace Element Research 2019, 193, 564 -573.
AMA StyleVladimir Sorokin, Anna B. Pshenichnikova, Sergei V. Kalenov, Nikolay A. Suyasov, Dmitry A. Skladnev. Comparison of the Wild-Type Obligate Methylotrophic Bacterium Methylophilus quaylei and its Isogenic Streptomycin-Resistant Mutant via Metal Nanoparticle Generation. Biological Trace Element Research. 2019; 193 (2):564-573.
Chicago/Turabian StyleVladimir Sorokin; Anna B. Pshenichnikova; Sergei V. Kalenov; Nikolay A. Suyasov; Dmitry A. Skladnev. 2019. "Comparison of the Wild-Type Obligate Methylotrophic Bacterium Methylophilus quaylei and its Isogenic Streptomycin-Resistant Mutant via Metal Nanoparticle Generation." Biological Trace Element Research 193, no. 2: 564-573.
Spray drying is appropriate for the preservation of halophilic microorganisms due to the nature of these microorganisms, as they survive in adverse environmental conditions by being encapsulated in salt crystals. Artificial neural networks were in this study used to optimize practically significant spray-drying regimes of the C50-carotenoids producer Halobacterium salinarum. Immediately after drying, the samples contained up to 54% halobacterial biomass and less than 5% moisture, and the level of preservation of carotenoids was 95–97%. The storage of biomass at 4 °C resulted in the gradual degradation of the carotenoids, which reached 58–64% in the best samples after 1 year. A comprehensive study of changes in halobacteria biomass after spray drying and the nature of the damage provided new data on the survival and preservation of cells and biologically active substances in the various spray-drying regimes and at different storage times.
Sergei V. Kalenov; Mariia G. Gordienko; Ekaterina D. Murzina; Daniil Y. Poberezhniy; Dmitry V. Baurin; Natalia E. Suzina; Alexander N. Morozov; Liubov M. Yakubovich; Alexey Belov; Victor I. Panfilov; Oksana V. Yarovaya; Michail M. Il’In; Vladimir Sorokin; Dmitry A. Skladnev. Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids. Extremophiles 2018, 22, 511 -523.
AMA StyleSergei V. Kalenov, Mariia G. Gordienko, Ekaterina D. Murzina, Daniil Y. Poberezhniy, Dmitry V. Baurin, Natalia E. Suzina, Alexander N. Morozov, Liubov M. Yakubovich, Alexey Belov, Victor I. Panfilov, Oksana V. Yarovaya, Michail M. Il’In, Vladimir Sorokin, Dmitry A. Skladnev. Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids. Extremophiles. 2018; 22 (3):511-523.
Chicago/Turabian StyleSergei V. Kalenov; Mariia G. Gordienko; Ekaterina D. Murzina; Daniil Y. Poberezhniy; Dmitry V. Baurin; Natalia E. Suzina; Alexander N. Morozov; Liubov M. Yakubovich; Alexey Belov; Victor I. Panfilov; Oksana V. Yarovaya; Michail M. Il’In; Vladimir Sorokin; Dmitry A. Skladnev. 2018. "Halobacterium salinarum storage and rehydration after spray drying and optimization of the processes for preservation of carotenoids." Extremophiles 22, no. 3: 511-523.
Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-staining purposes due to the spectral range covered (from orange to deep red). In contrast with other fluorescent dyes used so far, MitoTracker does not affect growth rate, and remains in cells after several washing steps and several generations in cell culture. The suggested dyes were tested on three archaeal (Hbt. salinarum, Haloferax sp., Halorubrum sp.) and two bacterial (Salicola sp., Halomonas sp.) strains of halophilic microorganisms. The new staining approach provides new insights into biology of Hbt. salinarum. We demonstrated the interconversion of rod-shaped cells of Hbt. salinarium to spheroplasts and submicron-sized spheres, as well as the cytoplasmic integrity of giant rod Hbt. salinarum species. By expanding the variety of tools available for halophile detection, MitoTracker dyes overcome long-standing limitations in fluorescence microscopy studies of halophiles.
Ivan Maslov; Andrey Bogorodskiy; Alexey Mishin; Ivan Okhrimenko; Ivan Gushchin; Sergei Kalenov; Norbert A. Dencher; Christoph Fahlke; Georg Büldt; Valentin Gordeliy; Thomas Gensch; Valentin Borshchevskiy. Efficient non-cytotoxic fluorescent staining of halophiles. Scientific Reports 2018, 8, 2549 .
AMA StyleIvan Maslov, Andrey Bogorodskiy, Alexey Mishin, Ivan Okhrimenko, Ivan Gushchin, Sergei Kalenov, Norbert A. Dencher, Christoph Fahlke, Georg Büldt, Valentin Gordeliy, Thomas Gensch, Valentin Borshchevskiy. Efficient non-cytotoxic fluorescent staining of halophiles. Scientific Reports. 2018; 8 (1):2549.
Chicago/Turabian StyleIvan Maslov; Andrey Bogorodskiy; Alexey Mishin; Ivan Okhrimenko; Ivan Gushchin; Sergei Kalenov; Norbert A. Dencher; Christoph Fahlke; Georg Büldt; Valentin Gordeliy; Thomas Gensch; Valentin Borshchevskiy. 2018. "Efficient non-cytotoxic fluorescent staining of halophiles." Scientific Reports 8, no. 1: 2549.
The method for producing AgNPs by granules of activated sludge micromycetes with enhanced tolerance to metal ion toxicity - Penicillium glabrum, Fusarium nivale and Fusarium oxysporum has been developed; the optimum conditions for AgNP biosynthesis being found: the Ag ion concentration, duration of the contact of microbial cells with silver ions, a growth phase of microorganisms, medium composition, a рН value, mixing conditions, and also lighting intensity. The effect of Cl, SO and HPO ions binding Ag ions was eliminated, that brought to significant increase of the yield of NPs. Under batch conditions, silver particles of 60-110 nanometers in size were formed with a 65% yield. It was established that the nanoparticles were covered with microbial cell membrane proteins composed up to 70% by weight of the NPs that prevented their aggregation. In addition, it was the first time stable AgNPs had been formed by continuous AgNP biosynthesis by living cells of F. oxysporum with an 80% yield for a long time.
Dmitry V. Tyupa; Sergei V. Kalenov; Marina Baurina; Liubov M. Yakubovich; Alexander Morozov; Ruslan M. Zakalyukin; Vladimir Sorokin; Dmitry A. Skladnev. Efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity. Enzyme and Microbial Technology 2016, 95, 137 -145.
AMA StyleDmitry V. Tyupa, Sergei V. Kalenov, Marina Baurina, Liubov M. Yakubovich, Alexander Morozov, Ruslan M. Zakalyukin, Vladimir Sorokin, Dmitry A. Skladnev. Efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity. Enzyme and Microbial Technology. 2016; 95 ():137-145.
Chicago/Turabian StyleDmitry V. Tyupa; Sergei V. Kalenov; Marina Baurina; Liubov M. Yakubovich; Alexander Morozov; Ruslan M. Zakalyukin; Vladimir Sorokin; Dmitry A. Skladnev. 2016. "Efficient continuous biosynthesis of silver nanoparticles by activated sludge micromycetes with enhanced tolerance to metal ion toxicity." Enzyme and Microbial Technology 95, no. : 137-145.
Highlights•Chemical/photochemical processes in the medium affect the BR synthesis.•HB are affected by products of chemical/photochemical oxidation of medium components.•Halobacterial cultivation with adsorbent is optimized to produce bacteriorhodopsin.•The use of adsorbents provides with obtaining qualitative halobacterial inoculum.•The cultivation methods leveling overstress of halobacteria are approved. AbstractSubmerged growth of Halobacterium salinarum and therefore synthesis of bacteriorhodopsin (BR) and carotenoids depend greatly on products of both chemical and/or photochemical oxidation of medium components and cellular metabolism which act as inhibitors. Some cultivation variants which allowed eliminating an adverse effect of inhibitors on biomass accumulation and BR synthesis are reviewed. The application of activated charcoal or ion exchange resin as adsorbents at preparing inoculums and the main cultivation stages was shown to allow controlling, namely lowering overstress of the halobacterial cells by metabolites. The halobacterial biomass containing BR up to 1,750 mg L−1 and the minimum amount of carotinoids that would facilitate BR isolation greatly was accumulated up to 45 g L−1 during eight-day cultivation with recycling cell suspension through adsorbent in a fed-batch mode. To control BR biosynthesis the express method of BR quantification based on colour shades of cell suspension was developed. Graphical abstract
Sergei V. Kalenov; Marina M. Baurina; Dmitry A. Skladnev; Alexander Ye. Kuznetsov. High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress. Journal of Biotechnology 2016, 233, 211 -218.
AMA StyleSergei V. Kalenov, Marina M. Baurina, Dmitry A. Skladnev, Alexander Ye. Kuznetsov. High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress. Journal of Biotechnology. 2016; 233 ():211-218.
Chicago/Turabian StyleSergei V. Kalenov; Marina M. Baurina; Dmitry A. Skladnev; Alexander Ye. Kuznetsov. 2016. "High-effective cultivation of Halobacterium salinarum providing with bacteriorhodopsin production under controlled stress." Journal of Biotechnology 233, no. : 211-218.
Silver removal from industrial waste waters by biosorbents is more profitable as compared with traditional sorbents such as ion-exchange resins or activated carbon. Silver biosorption by the filamentous fungi Penicillium glabrum, Fusarium nivale, and Fusarium oxysporum isolated from activated sludge was studied. These cultures formed granules which had tolerance to silver of high doses that allowed accumulating silver effectively. Through the selection of the optimal growth phase of the microorganisms, pH value, temperature, and chemical composition of the medium, it was possible to obtain the silver biosorbents having the adsorption capacity attaining nearly 988 mg/g. The process was best described with Langmuir isotherm; the kinetics of biosorption of silver by fungal granules obeyed the pseudo-second-order model.
D. V. Tyupa; S. V. Kalenov; M. M. Baurina; O. V. Kabanov; D. A. Skladnev; A. Ye Kuznetsov. Optimization of silver biosorption by fungi forming granules from aqueous solutions of silver nitrate. Clean Technologies and Environmental Policy 2016, 19, 53 -62.
AMA StyleD. V. Tyupa, S. V. Kalenov, M. M. Baurina, O. V. Kabanov, D. A. Skladnev, A. Ye Kuznetsov. Optimization of silver biosorption by fungi forming granules from aqueous solutions of silver nitrate. Clean Technologies and Environmental Policy. 2016; 19 (1):53-62.
Chicago/Turabian StyleD. V. Tyupa; S. V. Kalenov; M. M. Baurina; O. V. Kabanov; D. A. Skladnev; A. Ye Kuznetsov. 2016. "Optimization of silver biosorption by fungi forming granules from aqueous solutions of silver nitrate." Clean Technologies and Environmental Policy 19, no. 1: 53-62.
Microorganisms with high tolerance to toxic metal effects are essential for such processes as bioremediation, bioaccumulation, sewage and wastewater treatment, and also biosynthesis of metal nanoparticles (NPs).
Dmitry V. Tyupa; Sergei V. Kalenov; Marina M. Baurina; Victor I. Panfilov; Alexander Ye. Kuznetsov; Dmitry A. Skladnev. A facile method for formation of synthetic activated sludge granules with enhanced tolerance to metal ion toxicity. Journal of Chemical Technology & Biotechnology 2014, 91, 532 -538.
AMA StyleDmitry V. Tyupa, Sergei V. Kalenov, Marina M. Baurina, Victor I. Panfilov, Alexander Ye. Kuznetsov, Dmitry A. Skladnev. A facile method for formation of synthetic activated sludge granules with enhanced tolerance to metal ion toxicity. Journal of Chemical Technology & Biotechnology. 2014; 91 (2):532-538.
Chicago/Turabian StyleDmitry V. Tyupa; Sergei V. Kalenov; Marina M. Baurina; Victor I. Panfilov; Alexander Ye. Kuznetsov; Dmitry A. Skladnev. 2014. "A facile method for formation of synthetic activated sludge granules with enhanced tolerance to metal ion toxicity." Journal of Chemical Technology & Biotechnology 91, no. 2: 532-538.
Toxic impact of silver and uranium salts on activated sludge of wastewater treatment facilities has been studied. Some dominating cultures (an active nitrogen fixer Agrobacterium tumifaciens (A.t) and micromyces such as Fusarium nivale, Fusarium oxysporum, and Penicillium glabrum) have been isolated and identified as a result of selection of the activated sludge microorganisms being steadiest under stressful conditions. For these cultures, the lethal doses of silver amounted 1, 600, 50, and 300 µg/l and the lethal doses of uranium were 120, 1,500, 1,000, and 1,000 mg/l, respectively. A.tumifaciens is shown to be more sensitive to heavy metals than micromyces. Synthetic granular activated sludge was formed on the basis of three cultures of the isolated micromyces steadiest against stress. Its granules were much more resistant to silver than the whole native activated sludge was. The concentration of silver causing 50 % inhibition of synthetic granular activated sludge growth reached 160-170 μg/l as far as for the native activated sludge it came only to 100-110 μg/l.
Dmitry V. Tyupa; Sergei V. Kalenov; Dmitry A. Skladnev; Nikolay S. Khokhlachev; Marina M. Baurina; Alexander Ye. Kuznetsov. Toxic influence of silver and uranium salts on activated sludge of wastewater treatment plants and synthetic activated sludge associates modeled on its pure cultures. Bioprocess and Biosystems Engineering 2014, 38, 125 -135.
AMA StyleDmitry V. Tyupa, Sergei V. Kalenov, Dmitry A. Skladnev, Nikolay S. Khokhlachev, Marina M. Baurina, Alexander Ye. Kuznetsov. Toxic influence of silver and uranium salts on activated sludge of wastewater treatment plants and synthetic activated sludge associates modeled on its pure cultures. Bioprocess and Biosystems Engineering. 2014; 38 (1):125-135.
Chicago/Turabian StyleDmitry V. Tyupa; Sergei V. Kalenov; Dmitry A. Skladnev; Nikolay S. Khokhlachev; Marina M. Baurina; Alexander Ye. Kuznetsov. 2014. "Toxic influence of silver and uranium salts on activated sludge of wastewater treatment plants and synthetic activated sludge associates modeled on its pure cultures." Bioprocess and Biosystems Engineering 38, no. 1: 125-135.
Maintenance of the wastewater treatment plants and increasing the efficiency of existing aerobic biological reactors depend on the stability of activated sludge characteristics under varying wastewater parameters within significant limits and/or influence of some environmental factors. The steady microbial communities observed in biofilms and anaerobic granules of activated sludge can serve as successful samples of formation of the similar aerobic systems. The granular aerobic sludge obtained in the course of our researches is an ideal “plant” on treatment of biogenic pollution at both low and high concentrations. It demonstrates high ability for treatment and stability to adverse factors. To improve aerobic wastewater treatment characteristics, a possibility of using impact of stress conditions upon activated sludge has been studied. Under conditions of fractional hydrogen peroxide addition at diffused lighting, the granular aerobic activated sludge adapted to hydrogen peroxide has been obtained. This sludge has got good sedimentary properties and it differs from the control sample in the species diversity, improved treatment characteristics and also resistance to the stressor. It also endures an impact of one-time hydrogen peroxide addition up to 1.2–1.5 g H2O2/l. The conditions under which the steady aerobic granules of the diameter from 2 to 5 mm were formed with high treatment ability have been chosen. The granules were being stabilized at passages with hydrogen peroxide treatment and they endured up to 2.4–3.0 g/l of one-time H2O2 addition.
Nikolay S. Khokhlachev; Sergei V. Kalenov; Olga S. Zanina; Dmitry V. Tyupa; Marina Baurina; Alexander Kuznetsov. The role of stress agents as operating factors in formation and functioning of granular aerobic activated sludge at model domestic wastewater treatment. Bioprocess and Biosystems Engineering 2014, 37, 1771 -1779.
AMA StyleNikolay S. Khokhlachev, Sergei V. Kalenov, Olga S. Zanina, Dmitry V. Tyupa, Marina Baurina, Alexander Kuznetsov. The role of stress agents as operating factors in formation and functioning of granular aerobic activated sludge at model domestic wastewater treatment. Bioprocess and Biosystems Engineering. 2014; 37 (9):1771-1779.
Chicago/Turabian StyleNikolay S. Khokhlachev; Sergei V. Kalenov; Olga S. Zanina; Dmitry V. Tyupa; Marina Baurina; Alexander Kuznetsov. 2014. "The role of stress agents as operating factors in formation and functioning of granular aerobic activated sludge at model domestic wastewater treatment." Bioprocess and Biosystems Engineering 37, no. 9: 1771-1779.
Desorption of dissolved water and gases during the purification of organic liquids in microfiltration-desorption apparatuses is simulated. The problem of desorption from a liquid film of variable thickness flowing over a microfiltration membrane is solved, and its numerical and approximate analytical solutions are presented. The problem of desorption from a film running down an impermeable surface in additional purification system is also solved, and its analytical solution is presented. The solutions are used to determine the generalized variables controlling the extent of desorption. The conditions ensuring high purification efficiencies are found.
E. A. Dmitriev; A. M. Trushin; A. V. Tsvetnov; S. V. Kalenov. Mass transfer in a film flowing over semipermeable surfaces in microfiltration-desorption apparatuses. Theoretical Foundations of Chemical Engineering 2007, 41, 459 -466.
AMA StyleE. A. Dmitriev, A. M. Trushin, A. V. Tsvetnov, S. V. Kalenov. Mass transfer in a film flowing over semipermeable surfaces in microfiltration-desorption apparatuses. Theoretical Foundations of Chemical Engineering. 2007; 41 (5):459-466.
Chicago/Turabian StyleE. A. Dmitriev; A. M. Trushin; A. V. Tsvetnov; S. V. Kalenov. 2007. "Mass transfer in a film flowing over semipermeable surfaces in microfiltration-desorption apparatuses." Theoretical Foundations of Chemical Engineering 41, no. 5: 459-466.