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Angela Stufano
Animal Health and Zoonosis Doctoral Program, Department of Veterinary Medicine, University of Bari, 70010 Bari, Italy

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Journal article
Published: 27 July 2021 in Viruses
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Human T-cell lymphotropic virus type 1 (HTLV-1) infection affects millions of individuals worldwide and can lead to severe leukemia, myelopathy/tropical spastic paraparesis, and numerous other disorders. Pursuing a safe and effective immunotherapeutic approach, we compared the viral polyprotein and the human proteome with a sliding window approach in order to identify oligopeptide sequences unique to the virus. The immunological relevance of the viral unique oligopeptides was assessed by searching them in the immune epitope database (IEDB). We found that HTLV-1 has 15 peptide stretches each consisting of uniquely viral non-human pentapeptides which are ideal candidate for a safe and effective anti-HTLV-1 vaccine. Indeed, experimentally validated HTLV-1 epitopes, as retrieved from the IEDB, contain peptide sequences also present in a vast number of human proteins, thus potentially instituting the basis for cross-reactions. We found a potential for cross-reactivity between the virus and the human proteome and described an epitope platform to be used in order to avoid it, thus obtaining effective, specific, and safe immunization. Potential advantages for mRNA and peptide-based vaccine formulations are discussed.

ACS Style

Guglielmo Lucchese; Hamid Jahantigh; Leonarda De Benedictis; Piero Lovreglio; Angela Stufano. An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines. Viruses 2021, 13, 1461 .

AMA Style

Guglielmo Lucchese, Hamid Jahantigh, Leonarda De Benedictis, Piero Lovreglio, Angela Stufano. An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines. Viruses. 2021; 13 (8):1461.

Chicago/Turabian Style

Guglielmo Lucchese; Hamid Jahantigh; Leonarda De Benedictis; Piero Lovreglio; Angela Stufano. 2021. "An Epitope Platform for Safe and Effective HTLV-1-Immunization: Potential Applications for mRNA and Peptide-Based Vaccines." Viruses 13, no. 8: 1461.

Public health
Published: 07 July 2021 in Frontiers in Public Health
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Background: COVID-19 outbreaks in prisons and jails may affect both inmates and correctional workers. An observational study has been performed to investigate the efficacy of specific procedures and of a serial testing approach adopted for the COVID-19 prevention in an Italian correctional facility (Bari, Apulia) for inmates affected by chronic diseases. Methods: Two SARS-CoV-2 antigen testing campaigns were carried out for all the prisoners and correctional workers, including correctional officers (CO), administrative staff (AS), correctional health care workers (HCW), and operators working with people completing their sentence outside the prison (OOP). Antigen testing was conducted on nasopharyngeal swab specimens, using a fluorescence immunoassay for the qualitative detection of nucleocapsid SARS-CoV-2 antigen. All subjects positive to the antigen test underwent confirmation by rRT-PCR test. Results: In total, 426 new and residential inmates were tested during the first campaign and 480 during the second campaign. Only two new inmates resulted positive at the first campaign, while no positive cases were observed at the second campaign or outside of the testing campaigns. In total, 367 correctional workers were tested at the first campaign and 325 at the second. At the first, 4 CO and 2 HCW showed positive test results, while no new positive cases were observed at the second. Moreover, 1 CO and 1 HCW resulted positive outside of the testing campaigns for the onset of symptoms while at home. Conclusion: The implementation of a full risk management plan in a correctional facility, including both a strict protocol for the application of preventive measures and a serial testing approach, seems to be able to prevent COVID-19 outbreaks in both inmates and correctional workers.

ACS Style

Angela Stufano; Nicola Buonvino; Francesco Cagnazzo; Nicola Armenise; Daniela Pontrelli; Giovanna Curzio; Leonarda De Benedictis; Piero Lovreglio. Efficacy of the Measures Adopted to Prevent COVID-19 Outbreaks in an Italian Correctional Facility for Inmates Affected by Chronic Diseases. Frontiers in Public Health 2021, 9, 1 .

AMA Style

Angela Stufano, Nicola Buonvino, Francesco Cagnazzo, Nicola Armenise, Daniela Pontrelli, Giovanna Curzio, Leonarda De Benedictis, Piero Lovreglio. Efficacy of the Measures Adopted to Prevent COVID-19 Outbreaks in an Italian Correctional Facility for Inmates Affected by Chronic Diseases. Frontiers in Public Health. 2021; 9 ():1.

Chicago/Turabian Style

Angela Stufano; Nicola Buonvino; Francesco Cagnazzo; Nicola Armenise; Daniela Pontrelli; Giovanna Curzio; Leonarda De Benedictis; Piero Lovreglio. 2021. "Efficacy of the Measures Adopted to Prevent COVID-19 Outbreaks in an Italian Correctional Facility for Inmates Affected by Chronic Diseases." Frontiers in Public Health 9, no. : 1.

Journal article
Published: 21 May 2021 in Sustainability
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The COVID-19 incidence in 61 manufacturing plants in Europe (EU), North America (NA) and Latin-America (LATAM) was compared with the incidence observed in the countries where the plants are located in order to evaluate the application of an innovative model for COVID-19 risk management. Firstly, a network of local and global teams was created, including an external university occupational physician team for scientific support. In July 2020, global prevention guidelines for the homogenous management of the pandemic were applied, replacing different site or regional procedures. A tool for COVID-19 monitoring was implemented to investigate the relationship between the incidence rates inside and outside the plants. In the period of May–November 2020, 565 confirmed cases (EU 330, NA 141, LATAM 94) were observed among 20,646 workers with different jobs and tasks, and in the last two months 85% EU and 70% NA cases were recorded. Only in 10% of cases was a possible internal origin of the contagion not excluded. In the EU and NA, unlike LATAM, the COVID-19 incidence rates inside the sites punctually followed the rising trend outside. In conclusion, the model, combining a global approach with the local application of the measures, maintains the sustainability in the manufacturing industry.

ACS Style

Piero Lovreglio; Angela Stufano; Francesco Cagnazzo; Nicola Bartolomeo; Ivo Iavicoli. Application of an Innovative Model for the Risk Management of COVID-19 in a Multinational Manufacturing Company. Sustainability 2021, 13, 5771 .

AMA Style

Piero Lovreglio, Angela Stufano, Francesco Cagnazzo, Nicola Bartolomeo, Ivo Iavicoli. Application of an Innovative Model for the Risk Management of COVID-19 in a Multinational Manufacturing Company. Sustainability. 2021; 13 (11):5771.

Chicago/Turabian Style

Piero Lovreglio; Angela Stufano; Francesco Cagnazzo; Nicola Bartolomeo; Ivo Iavicoli. 2021. "Application of an Innovative Model for the Risk Management of COVID-19 in a Multinational Manufacturing Company." Sustainability 13, no. 11: 5771.

Journal article
Published: 20 April 2021
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Idiopathic pulmonary fibrosis (IPF) and asbestosis are pulmonary interstitial diseases that may present overlapping clinical aspects in the full-blown phase of the disease. For both clinical entities the gold standard for diagnosis is histological examination, but its execution poses ethical problems, especially when performed for preventive or forensic purposes. To evaluate the application of internationally accepted clinical, anamnestic and radiological criteria for differential diagnosis between asbestosis and IPF, and to assess the ability to discriminate between the two diseases. Even if clinically similar, the two diseases present extremely different prognostic and therapeutic perspectives. Two clinical cases of IPF are reported, in which the differential diagnosis was made by studying occupational exposure to asbestos, the onset and progression of clinical symptoms, and the identification of specific radiological elements by means of chest High Resolution Computed Tomography (HRCT). The diagnosis of IPF could be made on the basis of the absence of significant exposure to asbestos, the early onset and rapid progression of dyspnea and restrictive ventilatory defects, in association with a pulmonary radiological pattern characterized by peculiar elements such as honeycombing. The diagnostic procedure adopted to make a differential diagnosis with asbestosis provides practical clinical elements facilitating the differentiation between the two forms of pulmonary fibrosis, a fundamental aspect of the activity of the occupational physician.

ACS Style

Angela Stufano; Arnaldo Scardapane; Maria Pia Foschino Barbaro; Leonardo Soleo; Massimo Corradi; Piero Lovreglio. Clinical and radiological criteria for the differential diagnosis between asbestosis and idiopathic pulmonary fibrosis: Application in two cases. 2021, 112, 115 -122.

AMA Style

Angela Stufano, Arnaldo Scardapane, Maria Pia Foschino Barbaro, Leonardo Soleo, Massimo Corradi, Piero Lovreglio. Clinical and radiological criteria for the differential diagnosis between asbestosis and idiopathic pulmonary fibrosis: Application in two cases. . 2021; 112 (2):115-122.

Chicago/Turabian Style

Angela Stufano; Arnaldo Scardapane; Maria Pia Foschino Barbaro; Leonardo Soleo; Massimo Corradi; Piero Lovreglio. 2021. "Clinical and radiological criteria for the differential diagnosis between asbestosis and idiopathic pulmonary fibrosis: Application in two cases." 112, no. 2: 115-122.

Review
Published: 14 September 2020
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It is known in literature that women are frequently victims of murder, sexual assault and stalking. It is interesting to investigate the gender characterization when workplace bullying occurs. To investigate the phenomenon of workplace bullying and to understand if there are any relevant data about the gender aspects of workplace bullying by reviewing the literature in Italy. Review of articles on a sample of Italian workers. The research question followed the P.E.C.O. model and the article selection is based on PRISMA statement. Fourteen research strings were applied on: Pubmed, Microsoft Academic e Science Direct. A total of 32 articles were selected. There are few studies in which gender differences on workplace bullying and crimes related are analysed, in some cases it is considered in the research but not in the results section (n=6 articles). In the studies selected women are predominantly victims (n=10 articles), the aggressors instead are usually men (n=2 articles). The attacks suffered by women are frequently discriminations due to family management, children and gender related issues (n=6 articles). Men have the major consequences on mental health if compared to women (n=7 articles). Gender is relevant in the characterization of workplace bullying. There is a need for further investigation focusing on gender differences between perpetrators and victims of workplace bullying offences. A unique evaluation protocol is necessary for evaluating workplace bullying, the crimes linked to it and gender differences, to obtain a more accurate comparability of data.

ACS Style

Liliana Dassisti; Angela Stufano; Piero Lovreglio; Luigi Vimercati; Pasqua Loconsole; Ignazio Grattagliano. Donne e uomini, autori e vittime di mobbing in Italia: una revisione della letteratura. 2020, 111, 463 -477.

AMA Style

Liliana Dassisti, Angela Stufano, Piero Lovreglio, Luigi Vimercati, Pasqua Loconsole, Ignazio Grattagliano. Donne e uomini, autori e vittime di mobbing in Italia: una revisione della letteratura. . 2020; 111 (6):463-477.

Chicago/Turabian Style

Liliana Dassisti; Angela Stufano; Piero Lovreglio; Luigi Vimercati; Pasqua Loconsole; Ignazio Grattagliano. 2020. "Donne e uomini, autori e vittime di mobbing in Italia: una revisione della letteratura." 111, no. 6: 463-477.

Journal article
Published: 17 June 2020 in Toxicology Letters
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The study aims to investigate the influence of exposure to low concentrations of benzene on urinary biomarkers of nucleic acid oxidative damage and methylation. Benzene exposure was characterized for 93 coke production workers by measuring both airborne benzene and S-phenylmercapturic acid (SPMA) and unmodified benzene (U-B) in urine samples, collected at the end of the shift (ES) and at the next morning before shift (next BS). In the same urinary samples, biomarkers of nucleic acid oxidative damage and methylation were determined. Urinary concentrations of cotinine and creatinine were also determined to evaluate the smoking effect and to normalize urinary concentrations of analytes, respectively. The biomarkers of benzene internal dose, of oxidative damage (8-hydroxyy-7,8-dihydroguanine, 8-hydroxy-7,8-dihydroguanosine and 8-hydroxy-7,8−2'deoxyguanosine) and some of the biomarkers of nucleic acid methylation (5-Methyl-Cytosine, 1-Methyl-Guanine and 7-Methyl-Guanine) were higher in the ES than the next BS samples. Positive associations between ES 5-Methyl-Cytosine and both SPMA and U-B were found. In conclusion, occupational exposure to low levels of benzene seems to be related to urinary ES 5-Methyl-Cytosine that could be a possible biomarker to evaluate the changes of the nucleic acid methylation status.

ACS Style

Piero Lovreglio; Angela Stufano; Roberta Andreoli; Cesare Tomasi; Paola Cagnazzi; Anna Barbieri; Leonardo Soleo; Giuseppe De Palma. Urinary biomarkers of nucleic acid oxidation and methylation in workers exposed to low concentrations of benzene. Toxicology Letters 2020, 331, 235 -241.

AMA Style

Piero Lovreglio, Angela Stufano, Roberta Andreoli, Cesare Tomasi, Paola Cagnazzi, Anna Barbieri, Leonardo Soleo, Giuseppe De Palma. Urinary biomarkers of nucleic acid oxidation and methylation in workers exposed to low concentrations of benzene. Toxicology Letters. 2020; 331 ():235-241.

Chicago/Turabian Style

Piero Lovreglio; Angela Stufano; Roberta Andreoli; Cesare Tomasi; Paola Cagnazzi; Anna Barbieri; Leonardo Soleo; Giuseppe De Palma. 2020. "Urinary biomarkers of nucleic acid oxidation and methylation in workers exposed to low concentrations of benzene." Toxicology Letters 331, no. : 235-241.

Contact point
Published: 24 March 2020 in Contact Dermatitis
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ACS Style

Paolo Romita; Francesca Ambrogio; Angela Stufano; Piero Lovreglio; Nicola Mongelli; Pasquale Acquafredda; Caterina Foti. Occupational psoriasis after exposure to rockwool. Contact Dermatitis 2020, 83, 66 -67.

AMA Style

Paolo Romita, Francesca Ambrogio, Angela Stufano, Piero Lovreglio, Nicola Mongelli, Pasquale Acquafredda, Caterina Foti. Occupational psoriasis after exposure to rockwool. Contact Dermatitis. 2020; 83 (1):66-67.

Chicago/Turabian Style

Paolo Romita; Francesca Ambrogio; Angela Stufano; Piero Lovreglio; Nicola Mongelli; Pasquale Acquafredda; Caterina Foti. 2020. "Occupational psoriasis after exposure to rockwool." Contact Dermatitis 83, no. 1: 66-67.

Contact point
Published: 17 February 2020 in Contact Dermatitis
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ACS Style

Paolo Romita; Francesca Ambrogio; Serena De Prezzo; Giulia Gullo; Angela Stufano; Piero Lovreglio; Gianfranco Calogiuri; Caterina Foti. Occupational allergic contact dermatitis to Hydrangea spp. in an Italian florist. Contact Dermatitis 2020, 82, 393 -394.

AMA Style

Paolo Romita, Francesca Ambrogio, Serena De Prezzo, Giulia Gullo, Angela Stufano, Piero Lovreglio, Gianfranco Calogiuri, Caterina Foti. Occupational allergic contact dermatitis to Hydrangea spp. in an Italian florist. Contact Dermatitis. 2020; 82 (6):393-394.

Chicago/Turabian Style

Paolo Romita; Francesca Ambrogio; Serena De Prezzo; Giulia Gullo; Angela Stufano; Piero Lovreglio; Gianfranco Calogiuri; Caterina Foti. 2020. "Occupational allergic contact dermatitis to Hydrangea spp. in an Italian florist." Contact Dermatitis 82, no. 6: 393-394.

Research article
Published: 28 January 2020 in Inhalation Toxicology
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Objectives: The study aims to investigate occupational exposure to carbon fibers impregnated with epoxy resins (carbon fiber reinforced [CFR]) in workers at an airplane fuselage section construction plant, by environmental and biological monitoring.Materials and methods: Determination of airborne CFR was done by environmental sampling with active samplers, 11 of which were stationary and 19 personal samplings. The subsequent analyses were performed in the scanning electron microscope fitted with an X-ray microanalysis system (SEM-EDXA). Biological monitoring was carried out by determining CFR in exhaled breath condensate (EBC) collected from 19 male workers who wore personal environmental samplers (exposed workers) and from 10 male workers at the same factory who had no occupational exposure to CFR (internal controls). CFR analysis was done by SEM, applying the method used for determining asbestos fibers in aqueous samples.Results: The airborne CFR concentrations were found to be significantly higher (p = 0.03) at personal samplings (median value 7.01 ff/L, range 1.24-11.16 ff/L) than stationary samplings (median value 1.93 ff/L, range 0.55-10.09 ff/L). The aerodynamic diameters calculated starting from the length and geometric diameter of the sampled CFRs were always higher than 20 µm. CFR was not found in any of the EBC samples collected from the exposed workers and controls.Conclusions: Despite the evidence of occupational exposure to low concentrations of CFR, the absence of such fibers in the EBC in the exposed workers confirms their non-respirability, as expected based on their aerodynamic diameter.

ACS Style

Piero Lovreglio; Angela Stufano; Daniela Mele; Pasquale Acquafredda; Danilo Cottica; Francesco Gardinali; Luigi Vimercati; Leonardo Soleo; Giuseppe De Palma. Occupational exposure to carbon fibers impregnated with epoxy resins and evaluation of their respirability. Inhalation Toxicology 2020, 32, 63 -67.

AMA Style

Piero Lovreglio, Angela Stufano, Daniela Mele, Pasquale Acquafredda, Danilo Cottica, Francesco Gardinali, Luigi Vimercati, Leonardo Soleo, Giuseppe De Palma. Occupational exposure to carbon fibers impregnated with epoxy resins and evaluation of their respirability. Inhalation Toxicology. 2020; 32 (2):63-67.

Chicago/Turabian Style

Piero Lovreglio; Angela Stufano; Daniela Mele; Pasquale Acquafredda; Danilo Cottica; Francesco Gardinali; Luigi Vimercati; Leonardo Soleo; Giuseppe De Palma. 2020. "Occupational exposure to carbon fibers impregnated with epoxy resins and evaluation of their respirability." Inhalation Toxicology 32, no. 2: 63-67.

Review
Published: 28 December 2019 in Antioxidants
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Background: Homocysteine is a sulfur amino acid whose metabolism is activated in two pathways: remethylation to methionine, which requires folate and vitamin B12, and transsulfuration to cystathionine, which needs pyridoxal-5’-phosphate. High homocysteine level increases the risk of developing heart disease, stroke, peripheral vascular diseases, and cognitive impairment. Some evidence showed that exposure to these metals increased plasma homocysteine levels. Methods: A systematic review was carried out to clarify the relationship between homocysteine blood levels and exposure to toxic heavy metals (Lead, Cadmium, Mercury, and Chromium). Results: The results of this systematic review indicate that exposure to Pb, Cr, Cd, and Hg is connected with nonphysiological homocysteine levels or vitamin B12 and folate serum concentrations. Conclusions: These findings reinforce the importance of involvement in exposure to heavy metals in homocysteine metabolism. This supports the role of blood metals as potential upstream modifiable risk factors to prevent the development of other established risk factors as hyperhomocysteinemia.

ACS Style

Caterina Ledda; Emanuele Cannizzaro; Piero Lovreglio; Ermanno Vitale; Angela Stufano; Angelo Montana; Giovanni Li Volti; Venerando Rapisarda. Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism? Antioxidants 2019, 9, 30 .

AMA Style

Caterina Ledda, Emanuele Cannizzaro, Piero Lovreglio, Ermanno Vitale, Angela Stufano, Angelo Montana, Giovanni Li Volti, Venerando Rapisarda. Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism? Antioxidants. 2019; 9 (1):30.

Chicago/Turabian Style

Caterina Ledda; Emanuele Cannizzaro; Piero Lovreglio; Ermanno Vitale; Angela Stufano; Angelo Montana; Giovanni Li Volti; Venerando Rapisarda. 2019. "Exposure to Toxic Heavy Metals Can Influence Homocysteine Metabolism?" Antioxidants 9, no. 1: 30.

Journal article
Published: 28 November 2019 in Applied Sciences
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The study investigated the influence of genetic polymorphisms of the enzymes for DNA repair and detoxification of reactive intermediates on spontaneous and bleomycin-induced (BLM) genotoxic damage in 43 workers exposed to very low doses of ionizing radiation (IR) (mean cumulative dose 5.31 mSv) and 43 subjects with no occupational exposure to IR (controls). In all the subjects examined, the frequency of chromosome aberrations (CAs) and micronuclei (MN), both spontaneous and BLM-induced, the Comet assay parameters (tail intensity), the genotypic variants of the DNA repair enzymes XRCC1 (Arg194Trp, Arg280His, Arg399Gln), XRCC3 (Thr241Met), XPD (Lys751Gln), and of the detoxification enzymes GSTM1 and GSTT1 (null genotype) and BLMH (A1450G) were determined. Among the biomarkers considered, only the frequency of total CAs (p < 0.05), and in particular of chromosome breaks (p < 0.01), was found to be significantly higher in the exposed workers than the controls. The frequency of spontaneous MN was higher in subjects with at least one allelic variant in XRCC1 than in carriers of the wild-type, but again only in exposed workers (p = 0.046). Linear regression analysis showed a positive dependency of the frequency of spontaneous chromosome breaks on occupational exposure, and a dependency of the frequency of BLM-induced MN negative on occupational exposure and positive on alcohol consumption and the null GSTM1 genotype. In conclusion, the frequency of chromosome breaks seems to be a useful cytogenetic biomarker for exposure to very low doses of IR, while only the combined effect of different gene variants or genetic, occupational, and lifestyle habits factors seems to be able to modulate the genotoxic effect of very low doses of IR.

ACS Style

Angela Stufano; Patrizia Chiarappa; Rosanna Bagnulo; Ignazio Drago; Venerando Rapisarda; Caterina Ledda; Luigi Vimercati; Leonarda De Benedictis; Nicoletta Resta; Leonardo Soleo; Piero Lovreglio. Influence of Polymorphisms of DNA Repair and GST Genes on Genotoxic Damage and Mutagen Sensitivity in Workers Occupationally Exposed to Very Low Doses of Ionizing Radiation. Applied Sciences 2019, 9, 5175 .

AMA Style

Angela Stufano, Patrizia Chiarappa, Rosanna Bagnulo, Ignazio Drago, Venerando Rapisarda, Caterina Ledda, Luigi Vimercati, Leonarda De Benedictis, Nicoletta Resta, Leonardo Soleo, Piero Lovreglio. Influence of Polymorphisms of DNA Repair and GST Genes on Genotoxic Damage and Mutagen Sensitivity in Workers Occupationally Exposed to Very Low Doses of Ionizing Radiation. Applied Sciences. 2019; 9 (23):5175.

Chicago/Turabian Style

Angela Stufano; Patrizia Chiarappa; Rosanna Bagnulo; Ignazio Drago; Venerando Rapisarda; Caterina Ledda; Luigi Vimercati; Leonarda De Benedictis; Nicoletta Resta; Leonardo Soleo; Piero Lovreglio. 2019. "Influence of Polymorphisms of DNA Repair and GST Genes on Genotoxic Damage and Mutagen Sensitivity in Workers Occupationally Exposed to Very Low Doses of Ionizing Radiation." Applied Sciences 9, no. 23: 5175.

Case reports
Published: 31 July 2019 in Contact Dermatitis
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ACS Style

Caterina Foti; Paolo Romita; Angela Stufano; Piero Lovreglio; Luigi Vimercati; Stella Mazzoccoli; Annarita Antelmi; Cecilia Svedman. Occupational allergic contact dermatitis caused by 2‐butyl‐1,2‐benzisothiazol‐3(2 H )‐one in a metalworker. Contact Dermatitis 2019, 81, 463 -465.

AMA Style

Caterina Foti, Paolo Romita, Angela Stufano, Piero Lovreglio, Luigi Vimercati, Stella Mazzoccoli, Annarita Antelmi, Cecilia Svedman. Occupational allergic contact dermatitis caused by 2‐butyl‐1,2‐benzisothiazol‐3(2 H )‐one in a metalworker. Contact Dermatitis. 2019; 81 (6):463-465.

Chicago/Turabian Style

Caterina Foti; Paolo Romita; Angela Stufano; Piero Lovreglio; Luigi Vimercati; Stella Mazzoccoli; Annarita Antelmi; Cecilia Svedman. 2019. "Occupational allergic contact dermatitis caused by 2‐butyl‐1,2‐benzisothiazol‐3(2 H )‐one in a metalworker." Contact Dermatitis 81, no. 6: 463-465.

Journal article
Published: 01 January 2011 in Self/Nonself
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We explore the pentapeptide overlapping between human immunodeficiency virus (HIV) proteins and the human proteome. Our intent was to define viral peptides to be used in vaccines effective against different HIV strains, vaccines that are able to overcome the difficulties posed by the tendency of HIV to mutate, and that are also exempt from harmful collateral cross-reactions, as well as being repeatedly administrable to the global population. Analysis of HIV-1 envelope glycoprotein 160 (Env gp160) sequences revealed a set of 15 pentapeptides highly conserved among a number of retroviral sequences, and absent in the human proteome, thus representing unique molecular retroviral signatures. Use of these short viral peptide modules may represent the first concrete step toward the goal of a universal, safe and effective anti-HIV vaccine.

ACS Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. Searching for an effective, safe and universal anti-HIV vaccine: Finding the answer in just one short peptide. Self/Nonself 2011, 2, 49 -54.

AMA Style

Guglielmo Lucchese, Angela Stufano, Darja Kanduc. Searching for an effective, safe and universal anti-HIV vaccine: Finding the answer in just one short peptide. Self/Nonself. 2011; 2 (1):49-54.

Chicago/Turabian Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. 2011. "Searching for an effective, safe and universal anti-HIV vaccine: Finding the answer in just one short peptide." Self/Nonself 2, no. 1: 49-54.

Journal article
Published: 20 October 2010 in Self/Nonself
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The hypothesis that mimicry between a self and a microbial peptide antigen is strictly related to autoimmune pathology remains a debated concept in autoimmunity research. Clear evidence for a causal link between molecular mimicry and autoimmunity is still lacking. In recent studies we have demonstrated that viruses and bacteria share amino acid sequences with the human proteome at such a high extent that the molecular mimicry hypothesis becomes questionable as a causal factor in autoimmunity. Expanding upon our analysis, here we detail the bacterial peptide overlapping to the human proteome at the penta-, hexa-, hepta- and octapeptide levels by exact peptide matching analysis and demonstrate that there does not exist a single human protein that does not harbor a bacterial pentapeptide or hexapeptide motif. This finding suggests that molecular mimicry between a self and a microbial peptide antigen cannot be assumed as a basis for autoimmune pathologies. Moreover, the data are discussed in relation to the microbial immune escape phenomenon and the possible vaccine-related autoimmune effects.

ACS Style

Brett Trost; Guglielmo Lucchese; Angela Stufano; Mik Bickis; Anthony Kusalik; Darja Kanduc. No human protein is exempt from bacterial motifs, not even one. Self/Nonself 2010, 1, 328 -334.

AMA Style

Brett Trost, Guglielmo Lucchese, Angela Stufano, Mik Bickis, Anthony Kusalik, Darja Kanduc. No human protein is exempt from bacterial motifs, not even one. Self/Nonself. 2010; 1 (4):328-334.

Chicago/Turabian Style

Brett Trost; Guglielmo Lucchese; Angela Stufano; Mik Bickis; Anthony Kusalik; Darja Kanduc. 2010. "No human protein is exempt from bacterial motifs, not even one." Self/Nonself 1, no. 4: 328-334.

Methodology report
Published: 03 June 2010 in Journal of Biomedicine and Biotechnology
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Using the currently available proteome databases and based on the concept that a rare sequence is a potential epitope, epitopic sequences derived fromMycobacterium tuberculosiswere examined for similarity score to the proteins of the host in which the epitopes were defined. We found that: (i) most of the bacterial linear determinants had peptide fragment(s) that were rarely found in the host proteins and (ii) the relationship between low similarity and epitope definition appears potentially applicable to T-cell determinants. The data confirmed the hypothesis that low-sequence similarity shapes or determines the epitope definition at the molecular level and provides a potential tool for designing new approaches to prevent, diagnose, and treat tuberculosis and other infectious diseases.

ACS Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. Proposing Low-Similarity Peptide Vaccines againstMycobacterium tuberculosis. Journal of Biomedicine and Biotechnology 2010, 2010, 1 -8.

AMA Style

Guglielmo Lucchese, Angela Stufano, Darja Kanduc. Proposing Low-Similarity Peptide Vaccines againstMycobacterium tuberculosis. Journal of Biomedicine and Biotechnology. 2010; 2010 ():1-8.

Chicago/Turabian Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. 2010. "Proposing Low-Similarity Peptide Vaccines againstMycobacterium tuberculosis." Journal of Biomedicine and Biotechnology 2010, no. : 1-8.

Journal article
Published: 01 April 2010 in Self/Nonself
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Our previous research and a comprehensive meta-analysis of data from the literature on epitope mapping has revealed that the B cell epitope repertoire is allocated to rare peptide motifs, i.e., antigenic peptide sequences endowed with a low level of similarity to the host proteome. From a clinical point of view, low-similarity peptides able to evoke an immune response appear to be of special interest for the rational design of vaccines for poorly treatable diseases such as hepatitis-C virus (HCV) infection. Indeed, low similarity peptides would guarantee the highest specificity and lowest cross-reactivity, i.e., effectiveness without adverse side-effects. In this study, aimed at gaining further information for the development of effective anti-HCV peptide-based vaccines, the HCV epitopes recognized by human antibodies and currently catalogued in the Immune Epitope Data Base (IEDB) were examined for pentamer sequence similarities to the human proteome. We report that the analyzed HCV determinants are characterized by the presence of fragment absent from (or scarcely represented in) human proteins. These data confirm the low-similarity hypothesis, according to which a low-similarity to the host proteome defines the nonself character of microbial antigens and modulates peptide immunogenicity. Moreover, this study indicates a concrete and safe immunotherapeutic approach which might be used in a universal anti-HCV vaccine.

ACS Style

Angela Stufano; Giovanni Capone; Barbara Pesetti; Lorenzo Polimeno; Darja Kanduc. Clustering of rare peptide segments in the HCV immunome. Self/Nonself 2010, 1, 154 -162.

AMA Style

Angela Stufano, Giovanni Capone, Barbara Pesetti, Lorenzo Polimeno, Darja Kanduc. Clustering of rare peptide segments in the HCV immunome. Self/Nonself. 2010; 1 (2):154-162.

Chicago/Turabian Style

Angela Stufano; Giovanni Capone; Barbara Pesetti; Lorenzo Polimeno; Darja Kanduc. 2010. "Clustering of rare peptide segments in the HCV immunome." Self/Nonself 1, no. 2: 154-162.

Journal article
Published: 01 October 2009 in FEMS Immunology & Medical Microbiology
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The influenza A linear peptide epitopes recognized by murine antibodies, and currently cataloged at http://www.immuneepitope.org, were examined for the identity score to the host mouse proteome. It was found that almost all of the linear viral determinants are (or contain) regions formed by pentapeptide fragments with no or only very low similarity to the murine proteins. The present study adds to previous reports in suggesting a main role of amino acid sequence similarity in the modulation and definition of the B-cell epitope repertoire, inspiring innovative vaccine approaches able to avoid cross-reactive autoimmune collateral phenomena, and addressing future research in the study of immunity against the influenza A virus and infectious diseases in general.

ACS Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. Proteome-guided search for influenza A B-cell epitopes. FEMS Immunology & Medical Microbiology 2009, 57, 88 -92.

AMA Style

Guglielmo Lucchese, Angela Stufano, Darja Kanduc. Proteome-guided search for influenza A B-cell epitopes. FEMS Immunology & Medical Microbiology. 2009; 57 (1):88-92.

Chicago/Turabian Style

Guglielmo Lucchese; Angela Stufano; Darja Kanduc. 2009. "Proteome-guided search for influenza A B-cell epitopes." FEMS Immunology & Medical Microbiology 57, no. 1: 88-92.

Journal article
Published: 28 February 2009 in Experimental and Molecular Pathology
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The specific definition of the immunogenic peptide repertoire from tumor-associated-antigens (TAAs) is of crucial importance for designing effective immunotherapeutical treatments. Multiple mechanisms, at different levels and in complementary manners, contribute to the generation of immunogenic peptides. Using as an experimental model the prostate specific antigen (PSA) and a murine monoclonal antibody (MAb) raised against human PSA, the role of PSA peptide 1) similarity to the murine proteome, 2) affinity to H2-Ad/Ed molecules, and 3) proteasomal cleavage pattern were investigated. We report that the core of the interaction between the anti-PSA MAb ER-PR8 and the prostatic antigen is located in the pentapeptide motif PSA(27-31)GGWEC. The pentapeptide motif is characterized by having no sequence similarity to the murine proteome and a low number of potential proteolytic sites.

ACS Style

Angela Stufano; Darja Kanduc. Proteome-based epitopic peptide scanning along PSA. Experimental and Molecular Pathology 2009, 86, 36 -40.

AMA Style

Angela Stufano, Darja Kanduc. Proteome-based epitopic peptide scanning along PSA. Experimental and Molecular Pathology. 2009; 86 (1):36-40.

Chicago/Turabian Style

Angela Stufano; Darja Kanduc. 2009. "Proteome-based epitopic peptide scanning along PSA." Experimental and Molecular Pathology 86, no. 1: 36-40.

Journal article
Published: 31 October 2008 in Peptides
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Thirty viral proteomes were examined for amino acid sequence similarity to the human proteome, and, in parallel, a control of 30 sets of human proteins was analyzed for internal human overlapping. We find that all of the analyzed 30 viral proteomes, independently of their structural or pathogenic characteristics, present a high number of pentapeptide overlaps to the human proteome. Among the examined viruses, human T-lymphotropic virus 1, Rubella virus, and hepatitis C virus present the highest number of viral overlaps to the human proteome. The widespread and ample distribution of viral amino acid sequences through the human proteome indicates that viral and human proteins are formed of common peptide backbone units and suggests a fluid compositional chimerism in phylogenetic entities canonically classified distantly as viruses and Homo sapiens. Importantly, the massive viral to human peptide overlapping calls into question the possibility of a direct causal association between virus–host sharing of amino acid sequences and incitement to autoimmune reactions through molecular recognition of common motifs.

ACS Style

Darja Kanduc; Angela Stufano; Guglielmo Lucchese; Anthony Kusalik. Massive peptide sharing between viral and human proteomes. Peptides 2008, 29, 1755 -1766.

AMA Style

Darja Kanduc, Angela Stufano, Guglielmo Lucchese, Anthony Kusalik. Massive peptide sharing between viral and human proteomes. Peptides. 2008; 29 (10):1755-1766.

Chicago/Turabian Style

Darja Kanduc; Angela Stufano; Guglielmo Lucchese; Anthony Kusalik. 2008. "Massive peptide sharing between viral and human proteomes." Peptides 29, no. 10: 1755-1766.

Journal article
Published: 26 April 2007 in Amino Acids
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Summary. Our labs are focused on identifying amino acid sequences having the ability to react specifically with the functional binding site of a complementary antibody. Our epitopic definition is based on the analysis of the similarity level of antigenic amino acid sequences to the host proteome. Here, the similarity profile to the human proteome of an HCV E1 immunodominant epitope, i.e. the HCV E1315–328HRMAWDMMMNWSPT sequence, led to i) characterizing the immunoreactive HCV E1 315–328 region as a sequence endowed with a low level of similarity to human proteins; ii) defining 2 contiguous immunodominant linear determinants respectively located at the NH2 and COOH terminus of the conserved viral antigenic sequence. This study supports the hypothesis that low sequence similarity to the host’s proteome modulates the pool of epitopic amino acid sequences in a viral antigen, and appears of potential value in defining immunogenic viral peptide sequences to be used in immunotherapeutic approaches for HCV treatment.

ACS Style

L. Polimeno; A. Mittelman; L. Gennero; Antonio Ponzetto; Guglielmo Lucchese; Angela Stufano; A. Kusalik; D. Kanduc. Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis. Amino Acids 2007, 34, 479 -484.

AMA Style

L. Polimeno, A. Mittelman, L. Gennero, Antonio Ponzetto, Guglielmo Lucchese, Angela Stufano, A. Kusalik, D. Kanduc. Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis. Amino Acids. 2007; 34 (3):479-484.

Chicago/Turabian Style

L. Polimeno; A. Mittelman; L. Gennero; Antonio Ponzetto; Guglielmo Lucchese; Angela Stufano; A. Kusalik; D. Kanduc. 2007. "Sub-epitopic dissection of HCV E1315–328HRMAWDMMMNWSPT sequence by similarity analysis." Amino Acids 34, no. 3: 479-484.