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Vilma Kisnieriene
Department of Neurobiology and Biophysics, Institute of Biosciences, Life Sciences Center, Vilnius University, Sauletekio av. 7, LT-10257 Vilnius, Lithuania

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Journal article
Published: 29 March 2021 in Plants
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Inhibitors of human two-pore channels (TPC1 and TPC2), i.e., verapamil, tetrandrine, and NED-19, are promising medicines used in treatment of serious diseases. In the present study, the impact of these substances on action potentials (APs) and vacuolar channel activity was examined in the aquatic characean algae Nitellopsis obtusa and in the terrestrial liverwort Marchantia polymorpha. In both plant species, verapamil (20–300 µM) caused reduction of AP amplitudes, indicating impaired Ca2+ transport. In N. obtusa, it depolarized the AP excitation threshold and resting potential and prolonged AP duration. In isolated vacuoles of M. polymorpha, verapamil caused a reduction of the open probability of slow vacuolar SV/TPC channels but had almost no effect on K+ channels in the tonoplast of N. obtusa. In both species, tetrandrine (20–100 µM) evoked a pleiotropic effect: reduction of resting potential and AP amplitudes and prolongation of AP repolarization phases, especially in M. polymorpha, but it did not alter vacuolar SV/TPC activity. NED-19 (75 µM) caused both specific and unspecific effects on N. obtusa APs. In M. polymorpha, NED-19 increased the duration of repolarization. However, no inhibition of SV/TPC channels was observed in Marchantia vacuoles, but an increase in open probability and channel flickering. The results indicate an effect on Ca2+ -permeable channels governing plant excitation.

ACS Style

Mateusz Koselski; Vilmantas Pupkis; Kenji Hashimoto; Indre Lapeikaite; Agnieszka Hanaka; Piotr Wasko; Egle Plukaite; Kazuyuki Kuchitsu; Vilma Kisnieriene; Kazimierz Trebacz. Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean Macroalga Nitellopsis obtusa and the Early Terrestrial Liverwort Marchantia polymorpha. Plants 2021, 10, 647 .

AMA Style

Mateusz Koselski, Vilmantas Pupkis, Kenji Hashimoto, Indre Lapeikaite, Agnieszka Hanaka, Piotr Wasko, Egle Plukaite, Kazuyuki Kuchitsu, Vilma Kisnieriene, Kazimierz Trebacz. Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean Macroalga Nitellopsis obtusa and the Early Terrestrial Liverwort Marchantia polymorpha. Plants. 2021; 10 (4):647.

Chicago/Turabian Style

Mateusz Koselski; Vilmantas Pupkis; Kenji Hashimoto; Indre Lapeikaite; Agnieszka Hanaka; Piotr Wasko; Egle Plukaite; Kazuyuki Kuchitsu; Vilma Kisnieriene; Kazimierz Trebacz. 2021. "Impact of Mammalian Two-Pore Channel Inhibitors on Long-Distance Electrical Signals in the Characean Macroalga Nitellopsis obtusa and the Early Terrestrial Liverwort Marchantia polymorpha." Plants 10, no. 4: 647.

Research article
Published: 01 January 2018 in Functional Plant Biology
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The Nitellopsis obtusa (N.A.Desvaux) J.Groves cell provides a model system for complex investigation of instantaneous effects of various biologically active compounds (BC) on the generation of plant bioelectrical signals in vivo. Experimental evidence using multiple electrical signals as biomarkers of the effects of BC (acetylcholine, asparagine, glutamate, nicotine, aluminium, nickel and cadmium ions) is provided. The effect of BC on membrane transport systems involved in the cell excitability were tested by current clamp, voltage clamp and patch clamp methods. Membrane potential (MP) alterations and action potential (AP) patterns in response to BC were shown to represent the cell state. High discretisation frequency allows precise, high time resolution analysis of real-time processes measuring changes in excitation threshold, AP amplitude and velocity of repolarisation values after application of BC indicating the effect on ion channels involved in AP generation. Application of voltage clamp revealed that changes in AP peak value were caused not only by increment in averaged maximum amplitude of the Cl– current, but in prolonged Cl– channels’ opening time also. The cytoplasmic droplet can serve as a model system in which the effects of BC on single tonoplast ion channel can be studied by patch clamping. Investigation of electrical cell-to-cell communication revealed evidence on the electrical signal transduction through plasmodesmata.

ACS Style

Vilma Kisnieriene; Indre Lapeikaite; Vilmantas Pupkis. Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds. Functional Plant Biology 2018, 45, 132 -142.

AMA Style

Vilma Kisnieriene, Indre Lapeikaite, Vilmantas Pupkis. Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds. Functional Plant Biology. 2018; 45 (2):132-142.

Chicago/Turabian Style

Vilma Kisnieriene; Indre Lapeikaite; Vilmantas Pupkis. 2018. "Electrical signalling in Nitellopsis obtusa: potential biomarkers of biologically active compounds." Functional Plant Biology 45, no. 2: 132-142.

Journal article
Published: 13 February 2016 in Journal of Plant Research
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The effect of nickel (Ni) on the generation of plant bioelectrical signals was evaluated in Nitellopsis obtusa, a Characean model organism. Conventional glass-microelectrode technique and K(+)-anaesthesia method in current-clamp and voltage-clamp modes were used for the measurement and analysis of electrical parameters. Ni(2+) treatment rapidly influenced the action potential (AP) parameters namely, excitation threshold, AP peak and duration, membrane potential at various voltages and dynamics of ion currents. We conclude that altered electrical signaling pathway in the test organism constituted the early target for Ni toxicity imposition. The observed Ni interference could be ascribed to disturbed [Ca(2+)]cyt content, impaired Cl(-) and K(+) channels activity resulting in decreased excitability and repolarization rate in generated AP.

ACS Style

Vilma Kisnieriene; Indre Lapeikaite; Olga Sevriukova; Osvaldas Rukšėnas. The effects of Ni2+ on electrical signaling of Nitellopsis obtusa cells. Journal of Plant Research 2016, 129, 551 -558.

AMA Style

Vilma Kisnieriene, Indre Lapeikaite, Olga Sevriukova, Osvaldas Rukšėnas. The effects of Ni2+ on electrical signaling of Nitellopsis obtusa cells. Journal of Plant Research. 2016; 129 (3):551-558.

Chicago/Turabian Style

Vilma Kisnieriene; Indre Lapeikaite; Olga Sevriukova; Osvaldas Rukšėnas. 2016. "The effects of Ni2+ on electrical signaling of Nitellopsis obtusa cells." Journal of Plant Research 129, no. 3: 551-558.

Journal article
Published: 17 June 2014 in Biologija
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ACS Style

Vilma Kisnieriene; Jonas Burneika; Indre Lapeikaite; Olga Sevriukova; Algis Daktariunas. Investigation of membrane potential changes in Nitellopsis obtusa cells induced by blue and red light stimulation. Biologija 2014, 60, 1 .

AMA Style

Vilma Kisnieriene, Jonas Burneika, Indre Lapeikaite, Olga Sevriukova, Algis Daktariunas. Investigation of membrane potential changes in Nitellopsis obtusa cells induced by blue and red light stimulation. Biologija. 2014; 60 (2):1.

Chicago/Turabian Style

Vilma Kisnieriene; Jonas Burneika; Indre Lapeikaite; Olga Sevriukova; Algis Daktariunas. 2014. "Investigation of membrane potential changes in Nitellopsis obtusa cells induced by blue and red light stimulation." Biologija 60, no. 2: 1.

Journal article
Published: 01 April 2014 in Trace Elements and Electrolytes
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ACS Style

Olga Sevriukova; Auste Kanapeckaite; Vilma Kisnieriene; Rima Ladygienė; Indre Lapeikaite; Vidmantas Sakalauskas. Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution. Trace Elements and Electrolytes 2014, 31, 60 -66.

AMA Style

Olga Sevriukova, Auste Kanapeckaite, Vilma Kisnieriene, Rima Ladygienė, Indre Lapeikaite, Vidmantas Sakalauskas. Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution. Trace Elements and Electrolytes. 2014; 31 (4):60-66.

Chicago/Turabian Style

Olga Sevriukova; Auste Kanapeckaite; Vilma Kisnieriene; Rima Ladygienė; Indre Lapeikaite; Vidmantas Sakalauskas. 2014. "Modifying action of tritium on the charophytes bioelectrical response to anthropogenic pollution." Trace Elements and Electrolytes 31, no. 4: 60-66.

Journal article
Published: 01 December 2012 in Open Life Sciences
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The role of acetylcholine (ACh) as a signalling molecule in plants was investigated using a model system of Characeae cells. The effect of ACh on conductance of K+ channels in Nitella flexilis cells and on the action potential generation in Nitellopsis obtusa cells after H+-ATPase inhibition, where repolarization occurs after the opening of outward rectifying K+ channels, was investigated. Voltage-clamp method based on only one electrode impalement was used to evaluate the activity of separate potassium ion transport system at rest. We found that ACh at high concentrations (1 mM and 5 mM) activates K+ channels as the main membrane transport system at the resting state involved in electrogenesis of Characeaen membrane potential. We observed that ACh caused an increase in duration of AP repolarization of cells in K+ state when plasmalemma electrical characteristics are determined by large conductance K+ channels irrespective of whether AP were spontaneous or electrically evoked. These results indicate interference of ACh with electrical cellular signalling pathway in plants.

ACS Style

Vilma Kisnieriene; Tatiana Ditchenko; Anatoly Kudryashov; Vidmantas Sakalauskas; Vladimir Yurin; Osvaldas Rukšėnas. The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation. Open Life Sciences 2012, 7, 1066 -1075.

AMA Style

Vilma Kisnieriene, Tatiana Ditchenko, Anatoly Kudryashov, Vidmantas Sakalauskas, Vladimir Yurin, Osvaldas Rukšėnas. The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation. Open Life Sciences. 2012; 7 (6):1066-1075.

Chicago/Turabian Style

Vilma Kisnieriene; Tatiana Ditchenko; Anatoly Kudryashov; Vidmantas Sakalauskas; Vladimir Yurin; Osvaldas Rukšėnas. 2012. "The effect of acetylcholine on Characeae K+ channels at rest and during action potential generation." Open Life Sciences 7, no. 6: 1066-1075.

Journal article
Published: 01 September 2009 in Open Life Sciences
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Interrelations between the action of acetylcholine (ACh) and cadmium ions (Cd2+) on bioelectrogenesis of Nitellopsis obtusa cells were investigated. We analyzed repetitively triggered action potentials (AP), their reproducibility, shape and dynamics of membrane potential after AP induction. ACh significantly increased membrane permeability only at high concentrations (1 mM and 5 mM). Repolarisation level of action potential after the first stimulus was much more positive in all cells treated with ACh as compared to the control. Differences of membrane potentials between points just before the first and the second stimuli were 23.4±.0 mV (control); 40.4±5.9 mV (1 mM ACh solution) and 57.7 ± 8.5 mV (5 mM ACh solution). Cd2+ at 20 μM concentration was examined as a possible inhibitor of acetylcholinesterase (AChE) in vivo. We found that cadmium strengthens depolarizing effect of acetylcholine after the first stimulus. The highest velocity of AP repolarization was reduced after ACh application and Cd2+strengthened this effect. There were no differences in dynamics of membrane potential after repetitively triggered action potentials in ACh or ACh and Cd2+ solutions. This shows that cadmium in small concentration acts as inhibitor of acetylcholinesterase.

ACS Style

Vilma Kisnieriene; Vidmantas Sakalauskas; Aleksandras Pleskačiauskas; Vladimir Yurin; Osvaldas Rukšėnas. The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells. Open Life Sciences 2009, 4, 343 -350.

AMA Style

Vilma Kisnieriene, Vidmantas Sakalauskas, Aleksandras Pleskačiauskas, Vladimir Yurin, Osvaldas Rukšėnas. The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells. Open Life Sciences. 2009; 4 (3):343-350.

Chicago/Turabian Style

Vilma Kisnieriene; Vidmantas Sakalauskas; Aleksandras Pleskačiauskas; Vladimir Yurin; Osvaldas Rukšėnas. 2009. "The combined effect of Cd2+ and ACh on action potentials of Nitellopsis obtusa cells." Open Life Sciences 4, no. 3: 343-350.

Journal article
Published: 15 April 2008 in Informatics in Education
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Publisher: Vilnius University Institute of Data Science and Digital Technologies, Journal: Informatics in Education, Title: Information Technologies for Biology Education - Computerized Electrophysiology of Plant Cells, Authors: Vilma KISNIERIENE, Kastytis BEITAS, Vidmantas SAKALAUSKAS, Algis DAKTARIUNAS , Biology has moved from a bench-based discipline to a bioinformational science in modern times but application of computational and analytical methods of informatics in it is still a problem for many researchers and students of biology. We suggest to integrate cost effective and practical combination of the real and the virtual laboratories into the undergraduate biological science curriculum. This laboratory work illustrates passive and active electrical properties of plant cell membranes while introducing basic principles of electrophysiological recording, data acquisition and analysis. As the object for investigation in this laboratory work large cells of starry stonewort (Nitellopsis obtusa) were used. The simple program for experiment control and express visualization of recorded data was developed. Experiment proposed in this paper is easy implemented with a minimum of laboratory equipment, materials and gives an experience of computerized biological experiment.

ACS Style

Vilma Kisnieriene; Kastytis Beitas; Vidmantas Sakalauskas; Algis Daktariunas. Information Technologies for Biology Education: Computerized Electrophysiology of Plant Cells. Informatics in Education 2008, 7, 91 -104.

AMA Style

Vilma Kisnieriene, Kastytis Beitas, Vidmantas Sakalauskas, Algis Daktariunas. Information Technologies for Biology Education: Computerized Electrophysiology of Plant Cells. Informatics in Education. 2008; 7 (1):91-104.

Chicago/Turabian Style

Vilma Kisnieriene; Kastytis Beitas; Vidmantas Sakalauskas; Algis Daktariunas. 2008. "Information Technologies for Biology Education: Computerized Electrophysiology of Plant Cells." Informatics in Education 7, no. 1: 91-104.

Journal article
Published: 01 June 2007 in Open Life Sciences
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Aluminium induced membrane potential (Em) changes and potential changes during repolarization phase of the action potential (AP) in the internodal cells of Nitellopsis obtusa after blocking H+-ATPase activity by DCCD were investigated. Micromolar concentrations of DCCD are sufficient to give complete and irreversible inhibition of proton pumping. The membrane potential was measured by conventional glass-microelectrode technique. We found that the half-amplitude pulse duration differs significantly between standard conditions, after DCCD application, and after H+-ATPase blocking and subsequent Al3+ treatment: 4.9, 7.7 and 17.2 seconds, respectively. We propose that in the short term (2 hours) treatment of Al3+, the decrease in membrane potential was compensated for by H+-ATPase activity. Blocking H+-ATPase activity by DCCD can enhance the influence of Al3+ on the bioelectrical activity of cell membranes.

ACS Style

Vilma Kisnieriene; Vidmantas Sakalauskas. The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition. Open Life Sciences 2007, 2, 222 -232.

AMA Style

Vilma Kisnieriene, Vidmantas Sakalauskas. The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition. Open Life Sciences. 2007; 2 (2):222-232.

Chicago/Turabian Style

Vilma Kisnieriene; Vidmantas Sakalauskas. 2007. "The effect of aluminium on bioelectrical activity of the Nitellopsis obtusa cell membrane after H+-ATPase inhibition." Open Life Sciences 2, no. 2: 222-232.