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Mr. Matthew Davis
University of Florida

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0 Biotechnology
0 Genetics
0 Molecular Biology
0 Plant science
0 Agricultural Science

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I am currently an undergraduate student at the University of Florida.

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Journal article
Published: 23 November 2020 in Plants
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The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants.

ACS Style

Francesco Cappai; Alexandria Garcia; Ryan Cullen; Matthew Davis; Patricio R. Munoz. Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices. Plants 2020, 9, 1624 .

AMA Style

Francesco Cappai, Alexandria Garcia, Ryan Cullen, Matthew Davis, Patricio R. Munoz. Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices. Plants. 2020; 9 (11):1624.

Chicago/Turabian Style

Francesco Cappai; Alexandria Garcia; Ryan Cullen; Matthew Davis; Patricio R. Munoz. 2020. "Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices." Plants 9, no. 11: 1624.