This page has only limited features, please log in for full access.
Fertilizer-induced changes in soil nutrients regulate nitrogen (N) fixation in the terrestrial biosphere, but the influences of N and phosphorus (P) fertilization on the diazotroph communities in successive crop seasons were unclear. In this study, we assessed the effects of N and P (high vs. low doses) on the abundance and structure of N2-fixation communities after wheat and soybean harvest in a long-term (34 and 35 years) fertilization experiment. In both seasons, long-term N addition significantly decreased the abundance of nifH genes and 16S rDNA; in addition, high doses of N and P fertilizer decreased the richness of diazotrophs, whereas low doses did not. The proportion of the dominant genus, Bradyrhizobium, in the soybean season (86.0%) was higher than that in the wheat season (47.9%). Fertilization decreased diazotroph diversity and the relative abundance of Bradyrhizobium in the wheat season, but had insignificant effects in the soybean season. The addition of N, but not P, significantly changed the communities of both diazotrophs (at the genus level) and rhizobia (at the species level) in the two seasons. Soil pH was positively associated with nifH abundance and diazotrophic richness; soil NO3 − content was negatively correlated with diazotrophic richness and positively correlated with diversity. Soil pH and NO3 − content were the two main drivers shaping the soil diazotrophic community. Overall, long-term inorganic N had a greater influence than P on both diazotrophic abundance and community composition, and diazotrophic diversity was more clearly affected by fertilization in the wheat season than in the soybean season.
Jing Zhou; Mingchao Ma; Dawei Guan; Xin Jiang; Nianxin Zhang; Fengyue Shu; Yong Kong; Jun Li. Nitrogen has a greater influence than phosphorus on the diazotrophic community in two successive crop seasons in Northeast China. Scientific Reports 2021, 11, 1 -11.
AMA StyleJing Zhou, Mingchao Ma, Dawei Guan, Xin Jiang, Nianxin Zhang, Fengyue Shu, Yong Kong, Jun Li. Nitrogen has a greater influence than phosphorus on the diazotrophic community in two successive crop seasons in Northeast China. Scientific Reports. 2021; 11 (1):1-11.
Chicago/Turabian StyleJing Zhou; Mingchao Ma; Dawei Guan; Xin Jiang; Nianxin Zhang; Fengyue Shu; Yong Kong; Jun Li. 2021. "Nitrogen has a greater influence than phosphorus on the diazotrophic community in two successive crop seasons in Northeast China." Scientific Reports 11, no. 1: 1-11.
Increased inorganic nitrogen (N) and phosphorus (P) additions expected in the future will endanger the biodiversity and stability of agricultural ecosystems. In this context, a long-term fertilizer experiment (37 years) was set up in the black soil of northeast China. We examined interaction impacts of elevated fertilizer and host selection processes on arbuscular mycorrhizal fungi (AMF) communities in wheat rhizosphere soil using the Illumina MiSeq platform. The soil samples were subjected to five fertilization regimes: no fertilizer (CK) and low N (N1), low N plus low P (N1P1), high N (N2), and high N plus high P (N2P2) fertilizer. Long-term fertilization resulted in a significant shift in rhizosphere soil nutrient concentrations. The N fertilization (N1 and N2) did not significantly change rhizosphere AMF species diversity, but N plus P fertilization (N1P1 and N2P2) decreased it compared with CK. Non-metric multidimensional scaling showed that the rhizosphere AMF communities in CK, N1, N2, N1P1 and N2P2 treatments were distinct from each other. The AMF communities were predominantly composed of Glomeraceae, accounting for 30.0–39.1% of the sequences, and the relative abundance of family Glomeraceae was more abundance in fertilized soils, while family Paraglomeraceae were increased in N1 and N2 compared with CK. Analysis shown that AMF diversity was directly affected by soil C:P ratio but indirectly affected by plant under long-term fertilization. Overall, the results indicated that long-term N and P fertilization regimes changed rhizosphere AMF diversity and community composition, and rhizosphere AMF diversity was both affected by soil C:P ratio and plant.
Qingfeng Wang; Mingchao Ma; Xin Jiang; Dawei Guan; Dan Wei; Fengming Cao; Yaowei Kang; Changbin Chu; Shuhang Wu; Jun Li. Influence of 37 Years of Nitrogen and Phosphorus Fertilization on Composition of Rhizosphere Arbuscular Mycorrhizal Fungi Communities in Black Soil of Northeast China. Frontiers in Microbiology 2020, 11, 1 .
AMA StyleQingfeng Wang, Mingchao Ma, Xin Jiang, Dawei Guan, Dan Wei, Fengming Cao, Yaowei Kang, Changbin Chu, Shuhang Wu, Jun Li. Influence of 37 Years of Nitrogen and Phosphorus Fertilization on Composition of Rhizosphere Arbuscular Mycorrhizal Fungi Communities in Black Soil of Northeast China. Frontiers in Microbiology. 2020; 11 ():1.
Chicago/Turabian StyleQingfeng Wang; Mingchao Ma; Xin Jiang; Dawei Guan; Dan Wei; Fengming Cao; Yaowei Kang; Changbin Chu; Shuhang Wu; Jun Li. 2020. "Influence of 37 Years of Nitrogen and Phosphorus Fertilization on Composition of Rhizosphere Arbuscular Mycorrhizal Fungi Communities in Black Soil of Northeast China." Frontiers in Microbiology 11, no. : 1.
In this study, we investigated the physicochemical properties of soil, and the diversity and structure of the soil ammonia‐oxidizing archaea (AOA) community, when subjected to fertilizer treatments for over 35 years. We collected soil samples from a black soil fertilization trial in northeast China. Four treatments were tested: no fertilization (CK); manure (M); nitrogen (N), phosphorus (P), and potassium (K) chemical fertilizer (NPK); and N, P, and K plus M (MNPK). We employed 454 high‐throughput pyrosequencing to measure the response of the soil AOA community to the long‐term fertilization. The fertilization treatments had different impacts on the shifts in the soil properties and AOA community. The utilization of manure alleviated soil acidification and enhanced the soybean yield. The soil AOA abundance was increased greatly by inorganic and organic fertilizers. In addition, the community Chao1 and ACE were highest in the MNPK treatment. In terms of the AOA community composition, Thaumarchaeota and Crenarchaeota were the main AOA phyla in all samples. Compared with CK and M, the abundances of Thaumarchaeota were remarkably lower in the MNPK and NPK treatments. There were distinct shifts in the compositions of the AOA operational taxonomic units (OTUs) under different fertilization management practices. OTU51 was the dominant OTU in all treatments, except for NPK. OTU79 and OTU11 were relatively abundant OTUs in NPK. Only Nitrososphaera AOA were tracked from the black soil. Redundancy analysis indicated that the soil pH and soil available P were the two main factors that affected the AOA community structure. The abundances of AOA were positively correlated with the total N and available P concentrations, and negatively correlated with the soil pH.
Jianli Ding; Mingchao Ma; Xin Jiang; Yao Liu; Junzheng Zhang; Linna Suo; Lei Wang; Dan Wei; Jun Li. Effects of applying inorganic fertilizer and organic manure for 35 years on the structure and diversity of ammonia‐oxidizing archaea communities in a Chinese Mollisols field. MicrobiologyOpen 2019, 9, e00942 .
AMA StyleJianli Ding, Mingchao Ma, Xin Jiang, Yao Liu, Junzheng Zhang, Linna Suo, Lei Wang, Dan Wei, Jun Li. Effects of applying inorganic fertilizer and organic manure for 35 years on the structure and diversity of ammonia‐oxidizing archaea communities in a Chinese Mollisols field. MicrobiologyOpen. 2019; 9 (1):e00942.
Chicago/Turabian StyleJianli Ding; Mingchao Ma; Xin Jiang; Yao Liu; Junzheng Zhang; Linna Suo; Lei Wang; Dan Wei; Jun Li. 2019. "Effects of applying inorganic fertilizer and organic manure for 35 years on the structure and diversity of ammonia‐oxidizing archaea communities in a Chinese Mollisols field." MicrobiologyOpen 9, no. 1: e00942.
Phytotoxicity thresholds for heavy metals are derived from dose–response curves, which show the relationships between exposure dose and toxicity response. However, the results of tests or observations are commonly based on total heavy metal concentration, not the exposure dose that causes phytotoxicity; additionally, the phytotoxicity response differs with plant species. In the present study, the ethylenediaminetetraacetic acid (EDTA)-extractable copper (Cu) concentration was determined in order to evaluate Cu extractability. As two important horticultural food crops in Asia, tomato (Lycopersicon esculentum ‘Meifen No. 1’) and bok choy (Brassica rapa var. chinensis ‘Susheng 28’) were used to investigate Cu phytotoxicity in a wide range of Chinese soils with and without leaching treatment, after which relationships between Cu phytotoxicity thresholds based on EDTA-extractions and soil properties were established. The phytotoxicity thresholds showed that biomass of bok choy was more sensitive to Cu than tomato. Multiple linear regression analyses showed that soil factors, including organic carbon (OC), citrate dithionate extractable manganese (CD-Mn), cation exchange capacity (CEC), and CaCO3 explained over 85% of the variance in Cu phytotoxicity thresholds. The EDTA-extractable Cu dose–response relationships were further improved by incorporating soil properties. The new phytotoxicity predictive models indicated soil properties (soil pH, OC, CEC, and oxalate-extractable Mn) and EDTA-extractable Cu concentration explained more than 90% of the variance in the phytotoxicity response of tomato and bok choy biomass. The new phytotoxicity predictive models could be used to develop a reasonable remediation strategy for contaminated soils.
Bao Jiang; Yibing Ma; Guangyun Zhu; Jun Li. A New Model Describing Copper Dose–Toxicity to Tomato and Bok Choy Growth in a Wide Range of Soils. International Journal of Environmental Research and Public Health 2019, 16, 264 .
AMA StyleBao Jiang, Yibing Ma, Guangyun Zhu, Jun Li. A New Model Describing Copper Dose–Toxicity to Tomato and Bok Choy Growth in a Wide Range of Soils. International Journal of Environmental Research and Public Health. 2019; 16 (2):264.
Chicago/Turabian StyleBao Jiang; Yibing Ma; Guangyun Zhu; Jun Li. 2019. "A New Model Describing Copper Dose–Toxicity to Tomato and Bok Choy Growth in a Wide Range of Soils." International Journal of Environmental Research and Public Health 16, no. 2: 264.
Nitrogen (N) deposition can change ecosystem functions but little is known of long-term N-deposition and rhizosphere effects on the microbial community composition and enzymes activities related to the carbon (C) cycle in the black soil common to northeastern China. Here, we studied two enzyme activities involved in C cycles and microbial community composition in both the rhizosphere and bulk soil from a long-term (36-year) fertilization field experiment. N-addition significantly decreased bacterial abundance and phenol oxidase activity, but enhanced fungal abundance and peroxidase activity, in both the rhizosphere and bulk soil. The fungal diversity exhibited more obvious shifts than the bacterial diversity after long-term N-addition, resulting in significantly decreased bacterial and fungal diversity levels, except for bacterial diversity in the rhizosphere, which was not significantly changed. Moreover, the enzyme activities and the bacterial and fungal abundance levels were higher in the rhizosphere than in the bulk soil, suggesting a rhizosphere effect on microbial activities involved in the C cycle. There was a significant difference in the microbial community compositions among different N-addition levels. A lesser β-diversity response to N-addition was observed in the rhizosphere than in the bulk soil, and the responses of fungal communities were greater than those of bacterial communities. Our findings suggested that rhizosphere effects and fertilization regimes both have significant influences on microbial communities and soil enzyme activities, and that fungi were more sensitive than bacteria in responding to N-deposition.
Qingfeng Wang; Mingchao Ma; Xin Jiang; Dawei Guan; Dan Wei; Baisuo Zhao; Sanfeng Chen; Fengming Cao; Li Li; Xiaohong Yang; Jun Li. Impact of 36 years of nitrogen fertilization on microbial community composition and soil carbon cycling-related enzyme activities in rhizospheres and bulk soils in northeast China. Applied Soil Ecology 2019, 136, 148 -157.
AMA StyleQingfeng Wang, Mingchao Ma, Xin Jiang, Dawei Guan, Dan Wei, Baisuo Zhao, Sanfeng Chen, Fengming Cao, Li Li, Xiaohong Yang, Jun Li. Impact of 36 years of nitrogen fertilization on microbial community composition and soil carbon cycling-related enzyme activities in rhizospheres and bulk soils in northeast China. Applied Soil Ecology. 2019; 136 ():148-157.
Chicago/Turabian StyleQingfeng Wang; Mingchao Ma; Xin Jiang; Dawei Guan; Dan Wei; Baisuo Zhao; Sanfeng Chen; Fengming Cao; Li Li; Xiaohong Yang; Jun Li. 2019. "Impact of 36 years of nitrogen fertilization on microbial community composition and soil carbon cycling-related enzyme activities in rhizospheres and bulk soils in northeast China." Applied Soil Ecology 136, no. : 148-157.
Arbuscular mycorrhizal fungi (AMF) play vital roles in sustaining soil productivity and plant communities. However, adaption and differentiation of AMF in response to commonly used fertilization remain poorly understood. In this study, we showed that the AMF community composition was primarily driven by soil physiochemical changes associated with chronic inorganic and organic fertilization of 37 years in Mollisols. High-throughput sequencing indicated that inorganic fertilizer negatively affected AMF diversity and richness, implying a reduction of mutualism in plant–AMF symbiosis; however, a reverse trend was observed for the application of inorganic fertilizer combined with manure. With regards to AMF community composition, order Glomerales was dominant, but varied significantly among different fertilization treatments. All fertilization treatments decreased family Glomeraceae and genus Funneliformis, while Rhizophagus abundance increased. Plant-growth-promoting-microorganisms of family Claroideoglomeraceae and genus Claroideoglomus were stimulated by manure application, and likely benefited pathogen suppression and phosphorus (P) acquisition. Family Gigasporaceae and genus Gigaspora were negatively correlated with available P in soil. Additionally, redundancy analysis further suggested that soil available P, organic matter and pH were the most important factors in shaping AMF community composition. These results provide strong evidence for niche differentiation of phylogenetically distinct AMF populations under different fertilization regimes. Manure likely contributes to restoration and maintenance of plant–AMF symbiosis, and the balanced fertilization would favor the growth of beneficial AMF communities as one optimized management in support of sustainable agriculture in Mollisols.
Mingchao Ma; Marc Ongena; Qingfeng Wang; Dawei Guan; Fengming Cao; Xin Jiang; Jun Li. Chronic fertilization of 37 years alters the phylogenetic structure of soil arbuscular mycorrhizal fungi in Chinese Mollisols. AMB Express 2018, 8, 57 .
AMA StyleMingchao Ma, Marc Ongena, Qingfeng Wang, Dawei Guan, Fengming Cao, Xin Jiang, Jun Li. Chronic fertilization of 37 years alters the phylogenetic structure of soil arbuscular mycorrhizal fungi in Chinese Mollisols. AMB Express. 2018; 8 (1):57.
Chicago/Turabian StyleMingchao Ma; Marc Ongena; Qingfeng Wang; Dawei Guan; Fengming Cao; Xin Jiang; Jun Li. 2018. "Chronic fertilization of 37 years alters the phylogenetic structure of soil arbuscular mycorrhizal fungi in Chinese Mollisols." AMB Express 8, no. 1: 57.
The rhizosphere is a dynamic interface in which interactions among a myriad of microorganisms affect plants growth and tolerance to biotic and abiotic stress. Although rhizosphere effects on soil microbial communities have been widely investigated, few studies have evaluated such impacts of long-term fertilization on rhizosphere microbial communities in black soils common to northeast China. Here, we applied quantitative real-time polymerase chain reaction and high-throughput pyrosequencing to characterize rhizosphere and bulk soil bacterial communities in a long-term (36-year) fertilizer experiment. Soils were subjected to six treatments: CK (no fertilizer), N1 (150 kg urea ha−1 y−1), N2 (300 kg urea ha−1 y−1), M (18,600 kg horse manure ha−1 y−1), NPK (150 kg urea plus 33 kg P plus 62 kg K ha−1 y−1), and MNPK (M plus NPK). Inorganic fertilizer, especially N, decreased the 16S rRNA gene copy numbers and bacterial diversity in the rhizosphere and bulk soil, while manure fertilizer increased these values. Moreover, 16S rRNA gene copy numbers were higher and bacterial diversity was lower in the rhizosphere than the bulk soil, indicating that the maize rhizosphere had significant effects on bacterial diversity. The bacterial communities were predominantly composed of Proteobacteria and Acidobacteria in both the rhizosphere and bulk soil, but the rhizosphere and bulk soil communities were distinguished by principal coordinates analysis. Soil pH correlated with bacterial community composition and diversity in both rhizosphere and bulk soil. However, bacterial community composition in rhizosphere was more correlated with soil nutrient concentrations than in bulk soil under long-term fertilization. A redundancy analysis also indicated that soil pH, organic matter and available phosphorus concentrations were the most important factors in shaping bacterial communities in the maize rhizosphere. Our results revealed that long-term fertilization with increasing nutrients availability increased bacterial abundance, decreased biodiversity and changed bacterial composition in the rhizosphere.
Qingfeng Wang; Xin Jiang; Dawei Guan; Dan Wei; Baisuo Zhao; Mingchao Ma; Sanfeng Chen; Li Li; Fengming Cao; Jun Li. Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols. Applied Soil Ecology 2018, 125, 88 -96.
AMA StyleQingfeng Wang, Xin Jiang, Dawei Guan, Dan Wei, Baisuo Zhao, Mingchao Ma, Sanfeng Chen, Li Li, Fengming Cao, Jun Li. Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols. Applied Soil Ecology. 2018; 125 ():88-96.
Chicago/Turabian StyleQingfeng Wang; Xin Jiang; Dawei Guan; Dan Wei; Baisuo Zhao; Mingchao Ma; Sanfeng Chen; Li Li; Fengming Cao; Jun Li. 2018. "Long-term fertilization changes bacterial diversity and bacterial communities in the maize rhizosphere of Chinese Mollisols." Applied Soil Ecology 125, no. : 88-96.
How fungi respond to long‐term fertilization in Chinese Mollisols as sensitive indicators of soil fertility has received limited attention. To broaden our knowledge, we used high‐throughput pyrosequencing and quantitative PCR to explore the response of soil fungal community to long‐term chemical and organic fertilization strategies. Soils were collected in a 35‐year field experiment with four treatments: no fertilizer, chemical phosphorus, and potassium fertilizer (PK), chemical phosphorus, potassium, and nitrogen fertilizer (NPK), and chemical phosphorus and potassium fertilizer plus manure (MPK). All fertilization differently changed soil properties and fungal community. The MPK application benefited soil acidification alleviation and organic matter accumulation, as well as soybean yield. Moreover, the community richness indices (Chao1 and ACE) were higher under the MPK regimes, indicating the resilience of microbial diversity and stability. With regards to fungal community composition, the phylum Ascomycota was dominant in all samples, followed by Zygomycota, Basidiomycota, Chytridiomycota, and Glomeromycota. At each taxonomic level, the community composition dramatically differed under different fertilization strategies, leading to different soil quality. The NPK application caused a loss of Leotiomycetes but an increase in Eurotiomycetes, which might reduce the plant–fungal symbioses and increase nitrogen losses and greenhouse gas emissions. According to the linear discriminant analysis (LDA) coupled with effect size (LDA score > 3.0), the NPK application significantly increased the abundances of fungal taxa with known pathogenic traits, such as order Chaetothyriales, family Chaetothyriaceae and Pleosporaceae, and genera Corynespora, Bipolaris, and Cyphellophora. In contrast, these fungi were detected at low levels under the MPK regime. Soil organic matter and pH were the two most important contributors to fungal community composition.
Mingchao Ma; Xin Jiang; Qingfeng Wang; Marc Ongena; Dan Wei; Jianli Ding; Dawei Guan; Fengming Cao; Baisuo Zhao; Jun Li. Responses of fungal community composition to long-term chemical and organic fertilization strategies in Chinese Mollisols. MicrobiologyOpen 2018, 7, e00597 .
AMA StyleMingchao Ma, Xin Jiang, Qingfeng Wang, Marc Ongena, Dan Wei, Jianli Ding, Dawei Guan, Fengming Cao, Baisuo Zhao, Jun Li. Responses of fungal community composition to long-term chemical and organic fertilization strategies in Chinese Mollisols. MicrobiologyOpen. 2018; 7 (5):e00597.
Chicago/Turabian StyleMingchao Ma; Xin Jiang; Qingfeng Wang; Marc Ongena; Dan Wei; Jianli Ding; Dawei Guan; Fengming Cao; Baisuo Zhao; Jun Li. 2018. "Responses of fungal community composition to long-term chemical and organic fertilization strategies in Chinese Mollisols." MicrobiologyOpen 7, no. 5: e00597.
Bacteria play vital roles in soil biological fertility; however, it remains poorly understood about their response to long-term fertilization in Chinese Mollisols, especially when organic manure is substituted for inorganic nitrogen (N) fertilizer. To broaden our knowledge, high-throughput pyrosequencing and quantitative PCR were used to explore the impacts of inorganic fertilizer and manure on bacterial community composition in a 35-year field experiment of Chinese Mollisols. Soils were collected from four treatments: no fertilizer (CK), inorganic phosphorus (P) and potassium (K) fertilizer (PK), inorganic P, K, and N fertilizer (NPK), and inorganic P and K fertilizer plus manure (MPK). All fertilization differently changed soil properties. Compared with CK, the PK and NPK treatments acidified soil by significantly decreasing soil pH from 6.48 to 5.53 and 6.16, respectively, while MPK application showed no significant differences of soil pH, indicating alleviation of soil acidification. Moreover, all fertilization significantly increased soil organic matter (OM) and soybean yields, with the highest observed under MPK regime. In addition, the community composition at each taxonomic level varied considerably among the fertilization strategies. Bacterial taxa, associated with plant growth promotion, OM accumulation, disease suppression, and increased soil enzyme activity, were overrepresented in the MPK regime, while they were present at low abundant levels under NPK treatment, i.e. phyla Proteobacteria and Bacteroidetes, class Alphaproteobacteria, and genera Variovorax, Chthoniobacter, Massilia, Lysobacter, Catelliglobosispora and Steroidobacter. The application of MPK shifted soil bacterial community composition towards a better status, and such shifts were primarily derived from changes in soil pH and OM.
Mingchao Ma; Jing Zhou; Marc Ongena; Wenzheng Liu; Dan Wei; Baisuo Zhao; Dawei Guan; Xin Jiang; Jun Li. Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols. AMB Express 2018, 8, 20 .
AMA StyleMingchao Ma, Jing Zhou, Marc Ongena, Wenzheng Liu, Dan Wei, Baisuo Zhao, Dawei Guan, Xin Jiang, Jun Li. Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols. AMB Express. 2018; 8 (1):20.
Chicago/Turabian StyleMingchao Ma; Jing Zhou; Marc Ongena; Wenzheng Liu; Dan Wei; Baisuo Zhao; Dawei Guan; Xin Jiang; Jun Li. 2018. "Effect of long-term fertilization strategies on bacterial community composition in a 35-year field experiment of Chinese Mollisols." AMB Express 8, no. 1: 20.
Nodulation competition is a key factor that limits symbiotic nitrogen fixation between rhizobia and their host legumes. Soybean root exudates (SREs) are thought to act as signals that influence Bradyrhizobium ability to colonize roots and to survive in the rhizosphere, and thus they act as a key determinant of nodulation competitiveness. In order to find the competitiveness-related genes in B. diazoefficiens, the transcriptome of two SREs treated B. diazoefficiens with completely different nodulation abilities (B. diazoefficiens 4534 and B. diazoefficiens 4222) were sequenced and compared. In SREs treated strain 4534 (SREs-4534), 253 unigenes were up-regulated and 204 unigenes were down-regulated. In SREs treated strain 4534 (SREs-4222), the numbers of up- and down-regulated unigenes were 108 and 185, respectively. There were considerable differences between the SREs-4534 and SREs-4222 gene expression profiles. Some differentially expressed genes are associated with a two-component system (i.g., nodW, phyR-σEcfG), bacterial chemotaxis (i.g., cheA, unigene04832), ABC transport proteins (i.g., unigene02212), IAA (indole-3-acetic acid) metabolism (i.g., nthA, nthB), and metabolic fitness (i.g., put.), which may explain the higher nodulation competitiveness of B. diazoefficiens in the rhizosphere. Our results provide a comprehensive transcriptomic resource for SREs treated B. diazoefficiens and will facilitate further studies on competitiveness-related genes in B. diazoefficiens.
Yao Liu; Xin Jiang; Dawei Guan; Wei Zhou; Mingchao Ma; Baisuo Zhao; Fengming Cao; Li Li; Jun Li. Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates. Scientific Reports 2017, 7, 10946 .
AMA StyleYao Liu, Xin Jiang, Dawei Guan, Wei Zhou, Mingchao Ma, Baisuo Zhao, Fengming Cao, Li Li, Jun Li. Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates. Scientific Reports. 2017; 7 (1):10946.
Chicago/Turabian StyleYao Liu; Xin Jiang; Dawei Guan; Wei Zhou; Mingchao Ma; Baisuo Zhao; Fengming Cao; Li Li; Jun Li. 2017. "Transcriptional analysis of genes involved in competitive nodulation in Bradyrhizobium diazoefficiens at the presence of soybean root exudates." Scientific Reports 7, no. 1: 10946.
Long-term use of inorganic nitrogen (N) fertilization has greatly influenced the bacterial community in black soil of northeast China. It is unclear how N affects the bacterial community in two successive crop seasons in the same field for this soil type. We sampled soils from a long-term fertilizer experimental field in Harbin city with three N gradients. We applied sequencing and quantitative PCR targeting at the 16S rRNA gene to examine shifts in bacterial communities and test consistent shifts and driving-factors bacterial responses to elevated N additions. N addition decreased soil pH and bacterial 16S rDNA copy numbers, and increased soil N and crop yield. N addition consistently decreased bacterial diversity and altered bacterial community composition, by increasing the relative abundance of Proteobacteria, and decreasing that of Acidobacteria and Nitrospirae in both seasons. Consistent changes in the abundant classes and genera, and the structure of the bacterial communities across both seasons were observed. Our results suggest that increases in N inputs had consistent effects on the richness, diversity and composition of soil bacterial communities across the crop seasons in two continuous years, and the N addition and the subsequent edaphic changes were important factors in shaping bacterial community structures.
Jing Zhou; Xin Jiang; Dan Wei; Baisuo Zhao; Mingchao Ma; Sanfeng Chen; Fengming Cao; Delong Shen; Dawei Guan; Jun Li. Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China. Scientific Reports 2017, 7, 1 -10.
AMA StyleJing Zhou, Xin Jiang, Dan Wei, Baisuo Zhao, Mingchao Ma, Sanfeng Chen, Fengming Cao, Delong Shen, Dawei Guan, Jun Li. Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China. Scientific Reports. 2017; 7 (1):1-10.
Chicago/Turabian StyleJing Zhou; Xin Jiang; Dan Wei; Baisuo Zhao; Mingchao Ma; Sanfeng Chen; Fengming Cao; Delong Shen; Dawei Guan; Jun Li. 2017. "Consistent effects of nitrogen fertilization on soil bacterial communities in black soils for two crop seasons in China." Scientific Reports 7, no. 1: 1-10.
Although the effects of chemical fertilization management on microbial communities in soils have been well studied, few studies have examined such impacts of long-term chemical fertilizations on the microbial community in black soils common to northeast China. We applied high-throughput pyrosequencing and quantitative PCR of the 16S rRNA gene to investigate bacterial communities in a long-term fertilizer experiment started in 1980. The following fertilizer treatments were compared with control plots (no fertilizer): N1 (low nitrogen fertilizer), N2 (high nitrogen fertilizer), N1P1 (low nitrogen plus low phosphorus fertilizers) and N2P2 (high nitrogen plus high phosphorus fertilizers). All fertilization treatments resulted in decreases in soil pH and increases in wheat yield and concentrations of total nitrogen, organic matter and KCl-extractable NO3− and NH4+. Fertilization also led to a significant decrease in total 16S rRNA gene abundance and bacterial diversity. The phyla Proteobacteria, Acidobacteria and Actinobacteria dominated in all fertilized treatments. There was an increase in relative abundance of Actinobacteria, Proteobacteria, TM7 and Verrucomicrobia across all fertilized treatments compared to unfertilized controls, whereas phyla Acidobacteria and Nitrospirae decreased. The bacterial communities in unfertilized controls and lower-mineral fertilizers (i.e. N1 and N1P1) were predominantly composed of Acidobacteria, Actinobacteria and Proteobacteria, and separated from the communities where more concentrated fertilizer regimes were used (i.e. N2 and N2P2) based on principal coordinates analysis. Soil pH and NO3− concentration appeared to be the most important factors in shaping bacterial communities. Our findings suggested that long-term inorganic fertilizer regimes reduced the biodiversity and abundance of bacteria. The influence of more concentrated fertilizer treatments was greater than that of lower concentrations.
Jing Zhou; Dawei Guan; Baoku Zhou; Baisuo Zhao; Mingchao Ma; Jie Qin; Xin Jiang; Sanfeng Chen; Fengming Cao; Delong Shen; Jun Li. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biology and Biochemistry 2015, 90, 42 -51.
AMA StyleJing Zhou, Dawei Guan, Baoku Zhou, Baisuo Zhao, Mingchao Ma, Jie Qin, Xin Jiang, Sanfeng Chen, Fengming Cao, Delong Shen, Jun Li. Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China. Soil Biology and Biochemistry. 2015; 90 ():42-51.
Chicago/Turabian StyleJing Zhou; Dawei Guan; Baoku Zhou; Baisuo Zhao; Mingchao Ma; Jie Qin; Xin Jiang; Sanfeng Chen; Fengming Cao; Delong Shen; Jun Li. 2015. "Influence of 34-years of fertilization on bacterial communities in an intensively cultivated black soil in northeast China." Soil Biology and Biochemistry 90, no. : 42-51.