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Nano-enabled agriculture is an emerging hot topic. To facilitate the development of nano-enabled agriculture, reviews addressing or discussing the applications, knowledge gap, future research needs, and possible new research field of plant nanobiotechnology in agricultural production are encouraged. Here we review the following topics in plant nanobiotechnology for agriculture: 1) improving stress tolerance, 2) stress sensing and early detection, 3) targeted delivery and controlled release of agrochemicals, 4) transgenic events in non-model crop species, and 5) seed nanopriming. We discuss the knowledge gaps in these topics. Besides the use of nanomaterials for harvesting more electrons to improve photosynthetic performance, they could be used to convert nIR and UV to visible light to expand the light spectrum for photosynthesis. We discuss this approach to maintaining plant photosynthesis under light-insufficient conditions. Our aim in this review is to aid researchers to learn quickly how to use plant nanobiotechnology for improving agricultural production.
Honghong Wu; Zhaohu Li. Recent advances in nano-enabled agriculture for improving plant performance. The Crop Journal 2021, 1 .
AMA StyleHonghong Wu, Zhaohu Li. Recent advances in nano-enabled agriculture for improving plant performance. The Crop Journal. 2021; ():1.
Chicago/Turabian StyleHonghong Wu; Zhaohu Li. 2021. "Recent advances in nano-enabled agriculture for improving plant performance." The Crop Journal , no. : 1.
Background: Salinity is a big threat to agriculture by limiting crop production. Nanopriming (seed priming with nanomaterials) is an emerged approach to improve plant stress tolerance; however, our knowledge about the underlying mechanisms is limited. Results: We used cerium oxide nanoparticles (nanoceria) to prime rapeseeds and investigated the possible mechanisms behind nanoceria improved rapeseed salt tolerance. We synthesized and characterized polyacrylic acid coated nanoceria (PNC, 8.5 ± 0.2 nm, -43.3 ± 6.3 mV) and monitored its distribution in different tissues of the seed during the imbibition period (1, 3, 8h priming). Our results showed that compared with the no nanoparticle control, PNC nanopriming improved germination rate (12%) and biomass (41%) in rapeseeds under salt stress (200 mM NaCl). During the priming hours, PNC were located mostly in the seed coat, nevertheless the intensity of PNC in cotyledon and radicle was increased alongside with the increase of priming hours. During the priming hours, the amount of the absorbed water (52%, 14%, 12% increase at 1, 3, 8h priming, respectively) and the activities of α-amylase were significantly higher (175%, 309%, 295% increase at 1, 3, 8h priming, respectively) in PNC treatment than the control. PNC primed rapeseeds showed significantly lower content of MDA, H2O2, and •O2 — in both shoot and root than the control under salt stress. Also, under salt stress, PNC nanopriming enabled significantly higher K+ retention (29%) and also significantly lower Na+ accumulation (18.5%) and Na+/K+ ratio (37%) than the control. Conclusions: Our results suggested that besides the more absorbed water and increased α-amylase activities, PNC nanopriming improves salt tolerance in rapeseeds through maintaining ROS homeostasis and Na+/K+ ratio. It adds more knowledge regarding the mechanisms underlying nanopriming improved plant salt tolerance.
Mohammad Nauman Khan; Yanhui Li; Zaid Khan; Linlin Chen; Jiahao Liu; Jin Hu; Honghong Wu; Zhaohu Li. Nanoceria Seed Priming Improves Salt Tolerance in Rapeseed Through Modulating ROS Homeostasis and α-Amylase Activities. 2021, 1 .
AMA StyleMohammad Nauman Khan, Yanhui Li, Zaid Khan, Linlin Chen, Jiahao Liu, Jin Hu, Honghong Wu, Zhaohu Li. Nanoceria Seed Priming Improves Salt Tolerance in Rapeseed Through Modulating ROS Homeostasis and α-Amylase Activities. . 2021; ():1.
Chicago/Turabian StyleMohammad Nauman Khan; Yanhui Li; Zaid Khan; Linlin Chen; Jiahao Liu; Jin Hu; Honghong Wu; Zhaohu Li. 2021. "Nanoceria Seed Priming Improves Salt Tolerance in Rapeseed Through Modulating ROS Homeostasis and α-Amylase Activities." , no. : 1.
Background Salinity is a worldwide factor limiting the agricultural production. Cotton is an important cash crop; however, its yield and product quality are negatively affected by soil salinity. Use of nanomaterials such as cerium oxide nanoparticles (nanoceria) to improve plant tolerance to stress conditions, e.g. salinity, is an emerged approach in agricultural production. Nevertheless, to date, our knowledge about the role of nanoceria in cotton salt response and the behind mechanisms is still rare. Results We found that PNC (poly acrylic acid coated nanoceria) helped to improve cotton tolerance to salinity, showing better phenotypic performance, higher chlorophyll content (up to 68% increase) and biomass (up to 38% increase), and better photosynthetic performance such as carbon assimilation rate (up to 144% increase) in PNC treated cotton plants than the NNP (non-nanoparticle control) group. Under salinity stress, in consistent to the results of the enhanced activities of antioxidant enzymes, PNC treated cotton plants showed significant lower MDA (malondialdehyde, up to 44% decrease) content and reactive oxygen species (ROS) level such as hydrogen peroxide (H2O2, up to 79% decrease) than the NNP control group, both in the first and second true leaves. Further experiments showed that under salinity stress, PNC treated cotton plants had significant higher cytosolic K+ (up to 84% increase) and lower cytosolic Na+ (up to 77% decrease) fluorescent intensity in both the first and second true leaves than the NNP control group. This is further confirmed by the leaf ion content analysis, showed that PNC treated cotton plants maintained significant higher leaf K+ (up to 84% increase) and lower leaf Na+ content (up to 63% decrease), and thus the higher K+/Na+ ratio than the NNP control plants under salinity stress. Whereas no significant increase of mesophyll cell vacuolar Na+ intensity was observed in PNC treated plants than the NNP control under salinity stress, suggesting that the enhanced leaf K+ retention and leaf Na+ exclusion, but not leaf vacuolar Na+ sequestration are the main mechanisms behind PNC improved cotton salt tolerance. qPCR results showed that under salinity stress, the modulation of HKT1 but not SOS1 refers more to the PNC improved cotton leaf Na+ exclusion than the NNP control. Conclusions PNC enhanced leaf K+ retention and Na+ exclusion, but not vacuolar Na+ sequestration to enable better maintained cytosolic K+/Na+ homeostasis and thus to improve cotton salt tolerance. Our results add more knowledge for better understanding the complexity of plant-nanoceria interaction in terms of nano-enabled plant stress tolerance. Graphic abstract
Jiahao Liu; Guangjing Li; Linlin Chen; Jiangjiang Gu; Honghong Wu; Zhaohu Li. Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio. Journal of Nanobiotechnology 2021, 19, 1 -16.
AMA StyleJiahao Liu, Guangjing Li, Linlin Chen, Jiangjiang Gu, Honghong Wu, Zhaohu Li. Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio. Journal of Nanobiotechnology. 2021; 19 (1):1-16.
Chicago/Turabian StyleJiahao Liu; Guangjing Li; Linlin Chen; Jiangjiang Gu; Honghong Wu; Zhaohu Li. 2021. "Cerium oxide nanoparticles improve cotton salt tolerance by enabling better ability to maintain cytosolic K+/Na+ ratio." Journal of Nanobiotechnology 19, no. 1: 1-16.
Salinity is an issue impairing crop production across the globe. Under salinity stress, besides the osmotic stress and Na+ toxicity, ROS (reactive oxygen species) overaccumulation is a secondary stress which further impairs plant performance. Chloroplasts, mitochondria, the apoplast, and peroxisomes are the main ROS generation sites in salt-stressed plants. In this review, we summarize ROS generation, enzymatic and non-enzymatic antioxidant systems in salt-stressed plants, and the potential for plant biotechnology to maintain ROS homeostasis. Overall, this review summarizes the current understanding of ROS homeostasis of salt-stressed plants and highlights potential applications of plant nanobiotechnology to enhance plant tolerance to stresses.
Jiahao Liu; Chengcheng Fu; Guangjing Li; Mohammad Khan; Honghong Wu. ROS Homeostasis and Plant Salt Tolerance: Plant Nanobiotechnology Updates. Sustainability 2021, 13, 3552 .
AMA StyleJiahao Liu, Chengcheng Fu, Guangjing Li, Mohammad Khan, Honghong Wu. ROS Homeostasis and Plant Salt Tolerance: Plant Nanobiotechnology Updates. Sustainability. 2021; 13 (6):3552.
Chicago/Turabian StyleJiahao Liu; Chengcheng Fu; Guangjing Li; Mohammad Khan; Honghong Wu. 2021. "ROS Homeostasis and Plant Salt Tolerance: Plant Nanobiotechnology Updates." Sustainability 13, no. 6: 3552.
Editorial on the Research Topic New Insights Into Salinity Sensing, Signaling and Adaptation in Plants Plants under salt stress require additional energy supply to fuel salt tolerance mechanisms and growth. Bandehagh and Taylor establish that plants must strike a balance between energy supply and demand to maintain growth and development during salt stress. This review (1) summaries how salt stress affects different physiological and biochemical processes altering the abundance of different metabolites that are feeding into regular and alternative respiratory pathways and shunts; (2) critically analyses how these metabolic adjustments might help plants to tolerate the salt better; (3) identifies research gaps; and (4) proposes suggestions for future breeding programs targeting high energy-use efficiency. Farhat et al. studied oxidative phosphorylation of mitochondria by comparing mitochondria purified from the suspension cultures of a halophyte (Cakile maritima) and a closely related glycophyte (Arabidopsis thaliana) plant. The abundance of respiratory supercomplexes (monomeric complex I, dimeric complex III and I + III2 supercomplex) were found to be higher in halophyte mitochondria in comparison with glycophyte, implying the efficient electron transfer from complex I to complex III in halophyte mitochondria. Further, the stability of ATP synthase (complex V) was also found to be higher in the halophyte, suggesting that halophyte mitochondria are better equipped to supply the additional ATP required to support the salt stress response. The synthesis of organic compatible solutes is an important component for plant salt stress tolerance. In this regard, proline plays an important role in protecting plants from osmotic stress. Guan et al. show that overexpression of the genes from the D1-pyrroline-5-carboxylate synthetase enzyme, one of the key enzymes for proline synthesis, such as PvP5CS1 and PvP5CS2 increased salt tolerance in switchgrass. The relative expression levels of spermidine and spermine synthesis and metabolism-related genes were upregulated and downregulated in the PvP5CS overexpression (OE) transgenic plants and PvP5CS RNAi transformants, respectively. Their results also suggest that exogenously applied proline could accelerate polyamines metabolisms in salt-stressed switchgrass. Moreover, changes in lipid metabolism have previously been linked to responses to environmental stresses including salinity. In their study, Yu et al. investigated leaf and root lipidome profiles of two sweet potato (Ipomoea batatas L.) cultivars under salinity conditions and showed that salt tolerance is associated with changes in lipid metabolic processes. They also discovered the important role of phosphatidylserine (PS) in mediating enzyme activity, and exogenous application of PS alleviated the effects of NaCl tissue toxicity. Synthesis of organic osmolytes for osmotic adjustment is desirable; however, de nova synthesis of these molecules requires a high energy cost, and it cannot be sustainable while excluding salt. Alternatively, plants could employ tissue tolerance mechanisms so that they can use Na+ and Cl− for osmotic adjustment, saving energy instead of spending it on the synthesis of organic solutes. Using four rice varieties, Chakraborty et al. investigated the contribution of ionic discrimination and tissue tolerance in rice in terms of its energy cost. The results indicated that the two salt tolerance mechanisms, i.e., ionic selectivity and tissue tolerance, are distinct in rice. They also found that perhaps with a lower energy cost, unique rice varieties such as Kamini could effectively balance ionic discrimination vis-à-vis tissue tolerance to achieve salt tolerance. Furthermore, tissue specificity of employed salt tolerance mechanisms is known as an important factor which confers plant salinity stress tolerance. Liu, Shabala et al. investigated the differences in the operation of key ion transporters mediating ionic homeostasis in three rice varieties differing in salinity stress tolerance. The results showed that the superior K+ retention ability in both the mature and elongation zone of the rice root is the key trait conferring its differential salinity stress tolerance. They suggested that besides the superior ability to activate root H+-ATPase pump operation, this key trait is also related to the reduced sensitivity of K+ efflux channels to reactive oxygen species and the lower upregulation in OsGORK and higher upregulation of OsAKT1. A key trait which has long been recognized to improve salinity tolerance in many plants, is the maintenance of a low Na+/K+ ratio. Enhanced operation of salt overly sensitive 1 (SOS1; a Na+/H+ antiporter) has been shown to enable Na+ extrusion and thus confer salt tolerance in multiple plant species. Though SOS1 function at the root apex is well-established, how this transporter could function in the epidermis of the root mature zone and stele is unclear. Foster and Miklavcic employed biophysical modeling to show that SOS1 transport function is markedly different between the epidermis of the mature root zone and stele. In the epidermis, SOS1 restricts Na+ entry into the root cells reducing cytosolic Na+ levels of the root cells while in stele, SOS1 actively loads Na+ into the xylem, enhancing the flux of Na+ to the shoot. Besides SOS1 Na+/H+ antiporter, members of the HKT family proteins are well-known to protect plants against excess Na+ levels. Shohan et al. investigated the high-affinity K+ transporter HKT1;5 in different rice genotypes, including the salinity-sensitive Oryza genotype IR29, the salinity-tolerant Oryza genotype Pokkali, and the distantly related halophytic wild rice genotype Porteresia coarctata. A comparison of HKT1;5 sequences revealed four major amino acid substitutions, two of which were common for salinity-tolerant genotypes. Based on molecular modeling and structure validation, these two substitutions...
Honghong Wu; Camilla Beate Hill; Giovanni Stefano; Jayakumar Bose. Editorial: New Insights Into Salinity Sensing, Signaling and Adaptation in Plants. Frontiers in Plant Science 2021, 11, 1 .
AMA StyleHonghong Wu, Camilla Beate Hill, Giovanni Stefano, Jayakumar Bose. Editorial: New Insights Into Salinity Sensing, Signaling and Adaptation in Plants. Frontiers in Plant Science. 2021; 11 ():1.
Chicago/Turabian StyleHonghong Wu; Camilla Beate Hill; Giovanni Stefano; Jayakumar Bose. 2021. "Editorial: New Insights Into Salinity Sensing, Signaling and Adaptation in Plants." Frontiers in Plant Science 11, no. : 1.
BackgroundSalinity is a worldwide factor limiting the agricultural production. Cotton is an important cash crop; however, its yield and product quality are negatively affected by salinity. Using nanomaterials such as cerium oxide nanoparticles (nanoceria) to improve plant tolerance to stresses, e.g. salinity, is an emerged approach in agricultural production. Nevertheless, to date, our knowledge about the role of nanoceria in cotton salt response and the behind mechanisms is still rare. ResultsWe found that PNC (poly acrylic acid coated nanoceria) helped to improve cotton plant tolerance to salinity, showing the better phenotypic performance, the higher chlorophyll content and biomass, and the better photosynthetic performance in PNC treated cotton plants than the control group. Under salinity stress, in consistent to the results of the enhanced antioxidant enzyme activities, PNC treated cotton plants showed significant lower MDA content and ROS level than the control group, both in the first and second true leaf. Further experiments showed that under salinity stress, PNC treated cotton plants had significant higher cytosolic K+ and lower cytosolic Na+ fluorescent intensity in both the first and second true leaf than the control group. This is further confirmed by the leaf ion content analysis, showed that PNC treated cotton plants maintained significant higher leaf K+ and lower leaf Na+ content, and thus the higher K+/Na+ ratio than the control plants under salinity. Whereas no significant increase of vacuolar Na+ intensity was observed in PNC treated plants than the control under salinity, suggesting that PNC enhanced leaf K+ retention and leaf Na+ exclusion, but not leaf vacuolar Na+ sequestration are the main mechanisms behind the PNC improved cotton salt tolerance. qPCR results showed that under salinity stress, the modulation of HKT1 but not SOS1 refers more to the PNC improved cotton leaf Na+ exclusion than the control. ConclusionsNanoceria enhanced leaf K+ retention and Na+ exclusion, but not vacuolar Na+ sequestration are the main mechanisms behind the nanoceria improved cotton salt tolerance. Our results add more knowledge for better understanding the complexity of plant-nanoceria interaction in terms of nano-enabled plant stress tolerance.
Jiahao Liu; Guangjing Li; Linlin Chen; Jiangjiang Gu; Honghong Wu; Zhaohu Li. Cerium Oxide Nanoparticles Improve Cotton Salt Tolerance by Enabling Better Ability to Maintain Cytosolic K+/Na+ Ratio. 2021, 1 .
AMA StyleJiahao Liu, Guangjing Li, Linlin Chen, Jiangjiang Gu, Honghong Wu, Zhaohu Li. Cerium Oxide Nanoparticles Improve Cotton Salt Tolerance by Enabling Better Ability to Maintain Cytosolic K+/Na+ Ratio. . 2021; ():1.
Chicago/Turabian StyleJiahao Liu; Guangjing Li; Linlin Chen; Jiangjiang Gu; Honghong Wu; Zhaohu Li. 2021. "Cerium Oxide Nanoparticles Improve Cotton Salt Tolerance by Enabling Better Ability to Maintain Cytosolic K+/Na+ Ratio." , no. : 1.
Engineered nanomaterials interfaced with plant seeds can improve stress tolerance during the vulnerable seedling stage.
Jing An; Peiguang Hu; Fangjun Li; Honghong Wu; Yu Shen; Jason C. White; Xiaoli Tian; Zhaohu Li; Juan Pablo Giraldo. Emerging investigator series: molecular mechanisms of plant salinity stress tolerance improvement by seed priming with cerium oxide nanoparticles. Environmental Science: Nano 2020, 7, 2214 -2228.
AMA StyleJing An, Peiguang Hu, Fangjun Li, Honghong Wu, Yu Shen, Jason C. White, Xiaoli Tian, Zhaohu Li, Juan Pablo Giraldo. Emerging investigator series: molecular mechanisms of plant salinity stress tolerance improvement by seed priming with cerium oxide nanoparticles. Environmental Science: Nano. 2020; 7 (8):2214-2228.
Chicago/Turabian StyleJing An; Peiguang Hu; Fangjun Li; Honghong Wu; Yu Shen; Jason C. White; Xiaoli Tian; Zhaohu Li; Juan Pablo Giraldo. 2020. "Emerging investigator series: molecular mechanisms of plant salinity stress tolerance improvement by seed priming with cerium oxide nanoparticles." Environmental Science: Nano 7, no. 8: 2214-2228.
Near infrared (nIR) fluorescent single-walled carbon nanotubes (SWCNTs) were designed and interfaced with leaves of Arabidopsis thaliana plants to report hydrogen peroxide (H2O2), a key signaling molecule associated with the onset of plant stress. The sensor nIR fluorescence response (>900 nm) is quenched by H2O2 with selectivity against other stress-associated signaling molecules and within the plant physiological range (10-100 H2O2 µM). In vivo remote nIR imaging of H2O2 sensors enabled optical monitoring of plant health in response to stresses including UV-B light (-11%), high light (-6%), and a pathogen-related peptide (flg 22) (-10%) but not mechanical leaf wounding (<3%). The sensor's high biocompatibility was reflected on similar leaf cell death (<5%) and photosynthetic rates to controls without SWCNT. These optical nanosensors report early signs of stress and will improve our understanding of plant stress communication, provide novel tools for precision agriculture, and optimize the use of agrochemicals in the environment.
Honghong Wu; Robert Nißler; Victoria Morris; Niklas Herrmann; Peiguang Hu; Su-Ji Jeon; Sebastian Kruss; Juan Pablo Giraldo. Monitoring Plant Health with Near-Infrared Fluorescent H2O2 Nanosensors. Nano Letters 2020, 20, 2432 -2442.
AMA StyleHonghong Wu, Robert Nißler, Victoria Morris, Niklas Herrmann, Peiguang Hu, Su-Ji Jeon, Sebastian Kruss, Juan Pablo Giraldo. Monitoring Plant Health with Near-Infrared Fluorescent H2O2 Nanosensors. Nano Letters. 2020; 20 (4):2432-2442.
Chicago/Turabian StyleHonghong Wu; Robert Nißler; Victoria Morris; Niklas Herrmann; Peiguang Hu; Su-Ji Jeon; Sebastian Kruss; Juan Pablo Giraldo. 2020. "Monitoring Plant Health with Near-Infrared Fluorescent H2O2 Nanosensors." Nano Letters 20, no. 4: 2432-2442.
Sustainable agriculture is a key component of the effort to meet the increased food demand of a rapidly increasing global population. Nanobiotechnology is a promising tool for sustainable agriculture. However, rather than acting as nano-carriers, some nanoparticles (NPs) inherently enhance plant growth and stress tolerance. This biological role of nanoparticles depends on their physiochemical properties, application method (foliar delivery, hydroponics, soil) and the applied concentration. Here we review the effects of the different types, properties and concentrations of nanoparticles on plant growth and on various abiotic (salinity, drought, heat, high light, and heavy metals) and biotic (pathogens and herbivores) stresses. The ability of nanoparticles to stimulate plant growth by positive effects on seed germination, photosynthesis, root or shoot growth, and biomass or grain yield is also considered. The information presented herein will allow researchers within and outside the plant nanobiotechnology field to better select the appropriate nanoparticles as starting materials in agricultural applications. Ultimately, a shift from testing/utilizing existing nanoparticles to designing specific nanoparticles based on agriculture needs will facilitate the use of nanotechnology in sustainable agriculture.
Lijuan Zhao; Li Lu; Aodi Wang; Huiling Zhang; Min Huang; Honghong Wu; Baoshan Xing; Zhenyu Wang; Rong Ji. Nano-Biotechnology in Agriculture: Use of Nanomaterials to Promote Plant Growth and Stress Tolerance. Journal of Agricultural and Food Chemistry 2020, 68, 1935 -1947.
AMA StyleLijuan Zhao, Li Lu, Aodi Wang, Huiling Zhang, Min Huang, Honghong Wu, Baoshan Xing, Zhenyu Wang, Rong Ji. Nano-Biotechnology in Agriculture: Use of Nanomaterials to Promote Plant Growth and Stress Tolerance. Journal of Agricultural and Food Chemistry. 2020; 68 (7):1935-1947.
Chicago/Turabian StyleLijuan Zhao; Li Lu; Aodi Wang; Huiling Zhang; Min Huang; Honghong Wu; Baoshan Xing; Zhenyu Wang; Rong Ji. 2020. "Nano-Biotechnology in Agriculture: Use of Nanomaterials to Promote Plant Growth and Stress Tolerance." Journal of Agricultural and Food Chemistry 68, no. 7: 1935-1947.
Drought is one of the main limiting factors affecting tea plant yield and quality. Previous studies have reported that K+ (potassium) application significantly alleviated drought-induced damage in tea plants. However, the intrinsic mechanisms underlying K+-alleviated drought stress are still obscure. In our study, two contrasting varieties, Taicha12 (drought tolerant) and Fuyun6 (drought sensitive), were used to investigate the intrinsic mechanisms behind K+-alleviated drought stress in tea plants. In the present study, we compared with the case of tea plants under drought: higher water and chlorophyll contents were found in drought-stressed tea plants with an external K+ supply, confirming the role of externally supplied K+ in mitigating drought stress. We also found that an adequate K+ supply promoted Cl– accumulation in the mesophyll of Taicha12 (drought tolerant) over that of in Fuyun6 (drought sensitive). Moreover, Gly, Cys, Lys and Arg were not detected in Fuyun6 under ‘Drought’ or ‘Drought + K+’ conditions. Results showed that an exogenous supply of Arg and Val significantly alleviated drought-induced damage in Fuyun6, suggesting their role in K+-alleviated drought stress in tea plants. Collectively, our results show that chloride and amino acids are important components associated with K+-alleviated drought stress in tea plants.
Xianchen Zhang; Honghong Wu; Jingguang Chen; Linmu Chen; Xiaochun Wan. Chloride and amino acids are associated with K+-alleviated drought stress in tea (Camellia sinesis). Functional Plant Biology 2020, 47, 398 -408.
AMA StyleXianchen Zhang, Honghong Wu, Jingguang Chen, Linmu Chen, Xiaochun Wan. Chloride and amino acids are associated with K+-alleviated drought stress in tea (Camellia sinesis). Functional Plant Biology. 2020; 47 (5):398-408.
Chicago/Turabian StyleXianchen Zhang; Honghong Wu; Jingguang Chen; Linmu Chen; Xiaochun Wan. 2020. "Chloride and amino acids are associated with K+-alleviated drought stress in tea (Camellia sinesis)." Functional Plant Biology 47, no. 5: 398-408.
Salinity threatens agricultural production systems across the globe. While the major focus of plant researchers working in the field of salinity stress tolerance has always been on sodium and potassium, the transport patterns and physiological roles of Cl− in plant salt stress responses are studied much less. In recent years, the role of Cl− in plant salinity stress tolerance has been revisited and has received more attention. This review attempts to address the gap in knowledge of the role of Cl− transport in plant salinity stress tolerance. Cl− transport, Cl− exclusion, vacuolar Cl− sequestration, the specificity of mechanisms employed in different plant species to control shoot Cl− accumulation, and the identity of channels and transporters involved in Cl− transport in salt stressed plants are discussed. The importance of the electrochemical gradient across the tonoplast, for vacuolar Cl− sequestration, is highlighted. The toxicity of Cl− from CaCl2 is briefly reviewed separately to that of Cl− from NaCl.
Honghong Wu; Zhaohu Li. The Importance of Cl− Exclusion and Vacuolar Cl− Sequestration: Revisiting the Role of Cl− Transport in Plant Salt Tolerance. Frontiers in Plant Science 2019, 10, 1 .
AMA StyleHonghong Wu, Zhaohu Li. The Importance of Cl− Exclusion and Vacuolar Cl− Sequestration: Revisiting the Role of Cl− Transport in Plant Salt Tolerance. Frontiers in Plant Science. 2019; 10 ():1.
Chicago/Turabian StyleHonghong Wu; Zhaohu Li. 2019. "The Importance of Cl− Exclusion and Vacuolar Cl− Sequestration: Revisiting the Role of Cl− Transport in Plant Salt Tolerance." Frontiers in Plant Science 10, no. : 1.
Nanobiotechnology has the potential to enable smart plant sensors that communicate with and actuate electronic devices for improving plant productivity, optimize and automate water and agrochemical allocation, and enable high-throughput plant chemical phenotyping. Reducing crop loss due to environmental and pathogen-related stresses, improving resource use efficiency and selecting optimal plant traits are major challenges in plant agriculture industries worldwide. New technologies are required to accurately monitor, in real time and with high spatial and temporal resolution, plant physiological and developmental responses to their microenvironment. Nanomaterials are allowing the translation of plant chemical signals into digital information that can be monitored by standoff electronic devices. Herein, we discuss the design and interfacing of smart nanobiotechnology-based sensors that report plant signalling molecules associated with health status to agricultural and phenotyping devices via optical, wireless or electrical signals. We describe how nanomaterial-mediated delivery of genetically encoded sensors can act as tools for research and development of smart plant sensors. We assess performance parameters of smart nanobiotechnology-based sensors in plants (for example, resolution, sensitivity, accuracy and durability) including in vivo optical nanosensors and wearable nanoelectronic sensors. To conclude, we present an integrated and prospective vision on how nanotechnology could enable smart plant sensors that communicate with and actuate electronic devices for monitoring and optimizing individual plant productivity and resource use.
Juan Pablo Giraldo; Honghong Wu; Gregory Michael Newkirk; Sebastian Kruss. Nanobiotechnology approaches for engineering smart plant sensors. Nature Nanotechnology 2019, 14, 541 -553.
AMA StyleJuan Pablo Giraldo, Honghong Wu, Gregory Michael Newkirk, Sebastian Kruss. Nanobiotechnology approaches for engineering smart plant sensors. Nature Nanotechnology. 2019; 14 (6):541-553.
Chicago/Turabian StyleJuan Pablo Giraldo; Honghong Wu; Gregory Michael Newkirk; Sebastian Kruss. 2019. "Nanobiotechnology approaches for engineering smart plant sensors." Nature Nanotechnology 14, no. 6: 541-553.
Soil salinity is a major constraint for the global agricultural production. For many decades, Na+ exclusion from uptake has been the key trait targeted in breeding programs; yet, no major breakthrough in creating salt‐tolerant germplasm was achieved. In this work, we have combined the microelectrode ion flux estimation (MIFE) technique for non‐invasive ion flux measurements with confocal fluorescence dye imaging technique to screen 45 accessions of barley to reveal the relative contribution of Na+ exclusion from the cytosol to the apoplast and its vacuolar sequestration in the root apex, for the overall salinity stress tolerance. We show that Na+/H+ antiporter‐mediated Na+ extrusion from the root plays a minor role in the overall salt tolerance in barley. At the same time, a strong and positive correlation was found between root vacuolar Na+ sequestration ability and the overall salt tolerance. The inability of salt‐sensitive genotypes to sequester Na+ in root vacuoles was in contrast to significantly higher expression levels of both HvNHX 1 tonoplast Na+/H+ antiporters and HvVP 1 H+‐pumps compared with tolerant genotypes. These data are interpreted as a failure of sensitive varieties to prevent Na+ back‐leak into the cytosol and existence of a futile Na+ cycle at the tonoplast. Taken together, our results demonstrated that root vacuolar Na+ sequestration but not exclusion from uptake played the main role in barley salinity tolerance, and suggested that the focus of the breeding programs should be shifted from targeting genes mediating Na+ exclusion from uptake by roots to more efficient root vacuolar Na+ sequestration.
Honghong Wu; Lana Shabala; Meixue Zhou; Nana Su; Qi Wu; Tanveer Ul‐Haq; Juan Zhu; Stefano Mancuso; Elisa Azzarello; Sergey Shabala. Root vacuolar Na + sequestration but not exclusion from uptake correlates with barley salt tolerance. The Plant Journal 2019, 100, 55 -67.
AMA StyleHonghong Wu, Lana Shabala, Meixue Zhou, Nana Su, Qi Wu, Tanveer Ul‐Haq, Juan Zhu, Stefano Mancuso, Elisa Azzarello, Sergey Shabala. Root vacuolar Na + sequestration but not exclusion from uptake correlates with barley salt tolerance. The Plant Journal. 2019; 100 (1):55-67.
Chicago/Turabian StyleHonghong Wu; Lana Shabala; Meixue Zhou; Nana Su; Qi Wu; Tanveer Ul‐Haq; Juan Zhu; Stefano Mancuso; Elisa Azzarello; Sergey Shabala. 2019. "Root vacuolar Na + sequestration but not exclusion from uptake correlates with barley salt tolerance." The Plant Journal 100, no. 1: 55-67.
Tea plant is an important economic crop and is vulnerable to drought. A good understanding of tea drought tolerance mechanisms is required for breeding robust drought tolerant tea varieties. Previous studies showed mesophyll cells’ ability to maintain K+ is associated with its stress tolerance. Here, in this study, 12 tea varieties were used to investigate the role of mesophyll K+ retention ability towards tea drought stress tolerance. A strong and negative correlation (R2 = 0.8239, P < 0.001) was found between PEG (mimic drought stress)-induced K+ efflux from tea mesophyll cells and overall drought tolerance in 12 tea varieties. In agreement with this, a significantly higher retained leaf K+ content was found in drought tolerant than the sensitive tea varieties. Furthermore, exogenous applied K+ (5 mM) significantly alleviated drought-induced symptom in tea plants, further supporting our finding that mesophyll K+ retention is an important component for drought tolerance mechanisms in tea plants. Moreover, pharmacological experiments showed that the contribution of K+ outward rectifying channels and non-selective cation channels in controlling PEG-induced K+ efflux from mesophylls cells are varied between drought tolerant and sensitive tea varieties.
Xianchen Zhang; Honghong Wu; Linmu Chen; Ningning Wang; Chaoling Wei; Xiaochun Wan. Mesophyll cells’ ability to maintain potassium is correlated with drought tolerance in tea (Camellia sinensis). Plant Physiology and Biochemistry 2019, 136, 196 -203.
AMA StyleXianchen Zhang, Honghong Wu, Linmu Chen, Ningning Wang, Chaoling Wei, Xiaochun Wan. Mesophyll cells’ ability to maintain potassium is correlated with drought tolerance in tea (Camellia sinensis). Plant Physiology and Biochemistry. 2019; 136 ():196-203.
Chicago/Turabian StyleXianchen Zhang; Honghong Wu; Linmu Chen; Ningning Wang; Chaoling Wei; Xiaochun Wan. 2019. "Mesophyll cells’ ability to maintain potassium is correlated with drought tolerance in tea (Camellia sinensis)." Plant Physiology and Biochemistry 136, no. : 196-203.
Tea is a perennial leaf crop and grows in acidic soil with a pH usually ranging from 4.0 to 5.0. Tea leaves are rich in free amino acids that are strongly associated with nitrogen accumulation. Previous studies mainly have focused on NH4+ nutrition on tea growth and development; however, studies of the impact of NO3− nutrition on tea root plasma membrane (PM) H+-ATPase activities are limited. In our study, tea plants (cv. Shu chazao) were grown using NO3− as the nitrogen (N) source at pH 4.0 and 5.0. We found that compared with tea plants grown at pH 5.0, significantly lower N accumulation was found in tea plants grown at pH 4.0. Also, more depolarized PM potentials, and a lower net H+ fluxes, PM H+-ATPase activities, Km, Vmax and PM H+-ATPase protein levels were found at pH 4.0 than at pH 5.0. Furthermore, the PM H+-ATPase inhibitor, vanadate, significantly reduced nitrogen accumulation in tea roots and plants at pH 4.0 and 5.0, further confirming the role of PM H+-ATPase in nitrogen accumulation in tea plants. Exogenously applied ATP induced increased PM H+-ATPase activity in tea roots at both pH 4.0 and 5.0. Low pH impaired root respiration and decreased ATP concentration in tea roots grown under nitrate nutrient. Taken together, our results suggest PM H+-ATPase is involved in the lower accumulation of N in tea roots cultivated in nitrate nutrient at pH 4.0 than 5.0, and this is related to lower respiration rate and ATP concentrations.
Xianchen Zhang; Linmu Chen; Honghong Wu; Linlin Liu; Xiaochun Wan. Root plasma membrane H+-ATPase is involved in low pH-inhibited nitrogen accumulation in tea plants (Camellia sinensis L.). Plant Growth Regulation 2018, 86, 423 -432.
AMA StyleXianchen Zhang, Linmu Chen, Honghong Wu, Linlin Liu, Xiaochun Wan. Root plasma membrane H+-ATPase is involved in low pH-inhibited nitrogen accumulation in tea plants (Camellia sinensis L.). Plant Growth Regulation. 2018; 86 (3):423-432.
Chicago/Turabian StyleXianchen Zhang; Linmu Chen; Honghong Wu; Linlin Liu; Xiaochun Wan. 2018. "Root plasma membrane H+-ATPase is involved in low pH-inhibited nitrogen accumulation in tea plants (Camellia sinensis L.)." Plant Growth Regulation 86, no. 3: 423-432.
Reactive oxygen species (ROS) accumulation is a hallmark of plant abiotic stress response. ROS play a dual role in plants by acting as signaling molecules at low levels and damaging molecules at high levels. Accumulation of ROS in stressed plants can damage metabolites, enzymes, lipids, and DNA, causing a reduction of plant growth and yield. The ability of cerium oxide nanoparticles (nanoceria) to catalytically scavenge ROS in vivo provides a unique tool to understand and bioengineer plant abiotic stress tolerance. Here, we present a protocol to synthesize and characterize poly (acrylic) acid coated nanoceria (PNC), interface the nanoparticles with plants via leaf lamina infiltration, and monitor their distribution and ROS scavenging in vivo using confocal microscopy. Current molecular tools for manipulating ROS accumulation in plants are limited to model species and require laborious transformation methods. This protocol for in vivo ROS scavenging has the potential to be applied to wild type plants with broad leaves and leaf structure like Arabidopsis thaliana.
Gregory Michael Newkirk; Honghong Wu; Israel Santana; Juan Pablo Giraldo. Catalytic Scavenging of Plant Reactive Oxygen Species In Vivo by Anionic Cerium Oxide Nanoparticles. Journal of Visualized Experiments 2018, e58373 .
AMA StyleGregory Michael Newkirk, Honghong Wu, Israel Santana, Juan Pablo Giraldo. Catalytic Scavenging of Plant Reactive Oxygen Species In Vivo by Anionic Cerium Oxide Nanoparticles. Journal of Visualized Experiments. 2018; (138):e58373.
Chicago/Turabian StyleGregory Michael Newkirk; Honghong Wu; Israel Santana; Juan Pablo Giraldo. 2018. "Catalytic Scavenging of Plant Reactive Oxygen Species In Vivo by Anionic Cerium Oxide Nanoparticles." Journal of Visualized Experiments , no. 138: e58373.
Salinity is a global issue threatening agricultural production systems across the globe. While the major focus of plant salinity stress tolerance research has been on sodium, the transport and physiological roles of K+ in plant salt stress response has received less attention. This review attempts to bridge this knowledge gap. The major emphasis is on newly proposed K+ signalling roles and plant salt tolerance cell- and tissuespecificity. In addition to summarizing the importance of K+ retention for plant salt tolerance, we focus onaspects that were not the subject of previous reviews including (1) the importance of HAK/KUP family of transporters in K+ uptake in salt stressed plants and its possible linkage with Ca2+ and ROS signalling; (2) control of xylem K+ loading in salt stressed plants, control of phloem K+ recirculation in salt stressed plants and the potential importance of plant’s ability to efficiently coordinate K+ signals between root and shoot; (3) the buffering capacity of the vacuolar K+ pool; and (4) mechanisms of restoring the basal cytosolic K+ levels by coordinated activity of tonoplast K+-permeable channels. Overall, this review emphasises the need to fully understand the newly emerging roles of K+ and regulation of its transport for improving salinity stress tolerance in plants.
Honghong Wu; Xianchen Zhang; Juan Pablo Giraldo; Sergey Shabala. It is not all about sodium: revealing tissue specificity and signalling roles of potassium in plant responses to salt stress. Plant and Soil 2018, 431, 1 -17.
AMA StyleHonghong Wu, Xianchen Zhang, Juan Pablo Giraldo, Sergey Shabala. It is not all about sodium: revealing tissue specificity and signalling roles of potassium in plant responses to salt stress. Plant and Soil. 2018; 431 (1-2):1-17.
Chicago/Turabian StyleHonghong Wu; Xianchen Zhang; Juan Pablo Giraldo; Sergey Shabala. 2018. "It is not all about sodium: revealing tissue specificity and signalling roles of potassium in plant responses to salt stress." Plant and Soil 431, no. 1-2: 1-17.
Glucose is a major product of photosynthesis and a key energy source for cellular respiration in organisms. Herein, we enable in vivo optical glucose sensing in wild-type plants using a quantum dot (QD) ratiometric approach. The optical probe is formed by a pair of QDs: thioglycolic acid (TGA) capped QDs which remain invariable to glucose (acting as an internal fluorescent reference control) and boronic acid conjugated QDs (BA-QD) that quench their fluorescence in response to glucose. The fluorescence response of the QD probe is within the visible light window where photosynthetic tissues have a relatively low background. It is highly selective against other common sugars found in plants, and can be used to quantify glucose levels above 500 µM in planta within the physiological range. We demonstrate that the QD fluorescent probe reports glucose from single chloroplast to algae cells (Chara zeylanica), and plant leaf tissues (Arabidopsis thaliana) in vivo via confocal microscopy and to a standoff Raspberry Pi camera setup. QD based probes exhibit bright fluorescence, no photobleaching, tunable emission peak, and a size under plant cell wall porosity offering great potential for selective long-term monitoring of glucose in living photosynthetic organisms in situ.
Jinming Li; Honghong Wu; Israel Santana; Mackenzie Fahlgren; Juan Pablo Giraldo. Standoff Optical Glucose Sensing in Photosynthetic Organisms by a Quantum Dot Fluorescent Probe. ACS Applied Materials & Interfaces 2018, 10, 28279 -28289.
AMA StyleJinming Li, Honghong Wu, Israel Santana, Mackenzie Fahlgren, Juan Pablo Giraldo. Standoff Optical Glucose Sensing in Photosynthetic Organisms by a Quantum Dot Fluorescent Probe. ACS Applied Materials & Interfaces. 2018; 10 (34):28279-28289.
Chicago/Turabian StyleJinming Li; Honghong Wu; Israel Santana; Mackenzie Fahlgren; Juan Pablo Giraldo. 2018. "Standoff Optical Glucose Sensing in Photosynthetic Organisms by a Quantum Dot Fluorescent Probe." ACS Applied Materials & Interfaces 10, no. 34: 28279-28289.
Drought stress is one of the main factors limiting yield in tea plants. The plant cell's ability to preserve K+ homeostasis is an important strategy for coping with drought stress. Plasma membrane H+-ATPase in the mesophyll cell is important for maintaining membrane potential to regulate K+ transmembrane transport. However, no research to date has investigated the possible relationship between plasma membrane H+-ATPase and mesophyll K+ retention in tea plants under drought and subsequent rehydration conditions. In our experiment, drought stress inhibited plasma membrane H+-ATPase activities and induced net H+ influx, leading to membrane potential depolarization and inducing a massive K+ efflux in tea plant mesophyll cells. Subsequent rehydration increased plasma membrane H+-ATPase activity and induced net H+ efflux, leading to membrane potential hyperpolarization and thus lowering K+ loss. A first downregulated and then upregulated plasma membrane H+-ATPase protein expression level was also observed under drought and subsequent rehydration treatment, a finding in agreement with the change of measured plasma membrane H+-ATPase activities. Taken together, our results suggest that maintenance of mesophyll K+ in tea plants under drought and rehydration is associated with regulation of plasma membrane H+-ATPase activity.
Xianchen Zhang; Honghong Wu; Linmu Chen; Linlin Liu; Xiaochun Wan. Maintenance of mesophyll potassium and regulation of plasma membrane H+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration. The Crop Journal 2018, 6, 611 -620.
AMA StyleXianchen Zhang, Honghong Wu, Linmu Chen, Linlin Liu, Xiaochun Wan. Maintenance of mesophyll potassium and regulation of plasma membrane H+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration. The Crop Journal. 2018; 6 (6):611-620.
Chicago/Turabian StyleXianchen Zhang; Honghong Wu; Linmu Chen; Linlin Liu; Xiaochun Wan. 2018. "Maintenance of mesophyll potassium and regulation of plasma membrane H+-ATPase are associated with physiological responses of tea plants to drought and subsequent rehydration." The Crop Journal 6, no. 6: 611-620.
Tea plants grow in acidic soil, but to date, their intrinsic mechanisms of acidic stress tolerance have not been elucidated. Here, we assessed the tea plant response to growth on NH4+ nutrient media having different pH and iron levels. When grown in standard NH4+ nutrient solution (iron insufficient, 0.35 mg L−1 Fe2+), tea roots exhibited significantly lower nitrogen accumulation, plasma membrane H+‐ATPase activity, and protein levels; net H+ efflux was lower at pH 4.0 and 5.0 than at pH 6.0. Addition of 30 mg L−1 Fe2+ (iron sufficient, mimicking normal soil Fe2+ concentrations) to the NH4+ nutrient solution led to more efficient iron plaque formation on roots and increased root plasma membrane H+‐ATPase levels and activities at pH 4.0 and 5.0, compared to the pH 6.0 condition. Furthermore, plants grown at pH 4.0 and 5.0, with sufficient iron, exhibited significantly higher nitrogen accumulation than those grown at pH 6.0. Together, these results support the hypothesis that efficient iron plaque formation, on tea roots, is important for acidic stress tolerance. Furthermore, our findings establish that efficient iron plaque formation is linked to increased levels and activities of the tea root plasma membrane H+‐ATPase, under low pH conditions.
Xianchen Zhang; Honghong Wu; Lingmu Chen; Yeyun Li; Xiaochun Wan. Efficient iron plaque formation on tea (Camellia sinensis ) roots contributes to acidic stress tolerance. Journal of Integrative Plant Biology 2018, 61, 155 -167.
AMA StyleXianchen Zhang, Honghong Wu, Lingmu Chen, Yeyun Li, Xiaochun Wan. Efficient iron plaque formation on tea (Camellia sinensis ) roots contributes to acidic stress tolerance. Journal of Integrative Plant Biology. 2018; 61 (2):155-167.
Chicago/Turabian StyleXianchen Zhang; Honghong Wu; Lingmu Chen; Yeyun Li; Xiaochun Wan. 2018. "Efficient iron plaque formation on tea (Camellia sinensis ) roots contributes to acidic stress tolerance." Journal of Integrative Plant Biology 61, no. 2: 155-167.