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Ali Chenari Bouket Assistant Professor in Plant Pathology Plant Protection Research Department East Azarbaijan Agricultural and Natural Resources Research and Education Center (AREEO) East-Azarbaijan Tabriz-Azarshahr Highway, 2 Km after Police Station Postal code: 5355179854
Natural dyes have been used from ancient times for multiple purposes, most importantly in the field of textile dying. The increasing demand and excessive costs of natural dye extraction engendered the discovery of synthetic dyes from petrochemical compounds. Nowadays, they are dominating the textile market, with nearly 8 × 105 tons produced per year due to their wide range of color pigments and consistent coloration. Textile industries consume huge amounts of water in the dyeing processes, making it hard to treat the enormous quantities of this hazardous wastewater. Thus, they have harmful impacts when discharged in non-treated or partially treated forms in the environment (air, soil, plants and water), causing several human diseases. In the present work we focused on synthetic dyes. We started by studying their classification which depended on the nature of the manufactured fiber (cellulose, protein and synthetic fiber dyes). Then, we mentioned the characteristics of synthetic dyes, however, we focused more on their negative impacts on the ecosystem (soil, plants, water and air) and on humans. Lastly, we discussed the applied physical, chemical and biological strategies solely or in combination for textile dye wastewater treatments. Additionally, we described the newly established nanotechnology which achieves complete discharge decontamination.
Houda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences 2021, 11, 6255 .
AMA StyleHouda Ben Slama, Ali Chenari Bouket, Zeinab Pourhassan, Faizah N. Alenezi, Allaoua Silini, Hafsa Cherif-Silini, Tomasz Oszako, Lenka Luptakova, Patrycja Golińska, Lassaad Belbahri. Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods. Applied Sciences. 2021; 11 (14):6255.
Chicago/Turabian StyleHouda Ben Slama; Ali Chenari Bouket; Zeinab Pourhassan; Faizah N. Alenezi; Allaoua Silini; Hafsa Cherif-Silini; Tomasz Oszako; Lenka Luptakova; Patrycja Golińska; Lassaad Belbahri. 2021. "Diversity of Synthetic Dyes from Textile Industries, Discharge Impacts and Treatment Methods." Applied Sciences 11, no. 14: 6255.
Diverse vegetable oils are extracted from oleagenic trees and plants all over the world. In particular, olive oil represents a strategic socio-economic branch in the Mediterranean countries. These countries use either two or three-phase olive oil extraction systems. In this work, we focus on the by-products from three-phase olive oil extraction, which are the liquid olive mill wastewater (OMW) and the solid olive mill pomace (OMP) rejected in evaporative ponds. The disposal of this recalcitrant waste poses environmental problems such as the death of different species of insects and animals. In-depth ICP-OES analysis of the heavy metal composition of OMW and OMP revealed the presence of many metals ranging from non-toxic to highly toxic. The LC-HRMS characterization of these by-products indicated the presence of several secondary metabolites harmful to humans or to the environment. Thus, we aimed to identify OMW and OMP indigenous microbiota through metagenomics. The bacterial population was dominated by the Acetobacter (49.7%), Gluconobacter (17.3%), Gortzia (13.7%) and Nardonalla (5.3%) genera. The most abundant fungal genera were Nakazawaea, Saccharomyces, Lachancea and Candida. These microbial genera are responsible for OMW, OMP and soil toxicity alleviation.
Houda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences 2021, 11, 5131 .
AMA StyleHouda Ben Slama, Ali Chenari Bouket, Faizah N. Alenezi, Ameur Khardani, Lenka Luptakova, Armelle Vallat, Tomasz Oszako, Mostafa E. Rateb, Lassaad Belbahri. Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation. Applied Sciences. 2021; 11 (11):5131.
Chicago/Turabian StyleHouda Ben Slama; Ali Chenari Bouket; Faizah N. Alenezi; Ameur Khardani; Lenka Luptakova; Armelle Vallat; Tomasz Oszako; Mostafa E. Rateb; Lassaad Belbahri. 2021. "Olive Mill and Olive Pomace Evaporation Pond’s By-Products: Toxic Level Determination and Role of Indigenous Microbiota in Toxicity Alleviation." Applied Sciences 11, no. 11: 5131.
Plant growth promoting bacteria (PGPB) have been the target of intensive research studies toward their efficient use in the field as biofertilizers, biocontrol, and bioremediation agents among numerous other applications. Recent trends in the field of PGPB research led to the development of versatile multifaceted PGPB that can be used in different field conditions such as biocontrol of plant pathogens in metal contaminated soils. Unfortunately, all these research efforts lead to the development of PGPB that failed to perform in salty environments. Therefore, it is urgently needed to address this drawback of these PGPB toward their efficient performance in salinity context. In this paper we provide a review of state-of-the-art research in the field of PGPB and propose a road map for the development of next generation versatile and multifaceted PGPB that can perform in salinity. Beyond soil desalinization, our study paves the way towards the development of PGPB able to provide services in diverse salty environments such as heavy metal contaminated, or pathogen threatened. Smart development of salinity adapted next generation biofertilizers will inevitably allow for mitigation and alleviation of biotic and abiotic threats to plant productivity in salty environments.
Hafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability 2021, 13, 4422 .
AMA StyleHafsa Cherif-Silini, Allaoua Silini, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Nawel Bouremani, Justyna Anna Nowakowska, Tomasz Oszako, Lassaad Belbahri. Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application. Sustainability. 2021; 13 (8):4422.
Chicago/Turabian StyleHafsa Cherif-Silini; Allaoua Silini; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Nawel Bouremani; Justyna Anna Nowakowska; Tomasz Oszako; Lassaad Belbahri. 2021. "Tailoring Next Generation Plant Growth Promoting Microorganisms as Versatile Tools beyond Soil Desalinization: A Road Map towards Field Application." Sustainability 13, no. 8: 4422.
There is a great interest in mitigating soil salinity that limits plant growth and productivity. In this study, eighty-nine strains were isolated from the rhizosphere and endosphere of two halophyte species (Suaeda mollis and Salsola tetrandra) collected from three chotts in Algeria. They were screened for diverse plant growth-promoting traits, antifungal activity and tolerance to different physico-chemical conditions (pH, PEG, and NaCl) to evaluate their efficiency in mitigating salt stress and enhancing the growth of Arabidopsis thaliana and durum wheat under NaCl–stress conditions. Three bacterial strains BR5, OR15, and RB13 were finally selected and identified as Bacillus atropheus. The Bacterial strains (separately and combined) were then used for inoculating Arabidopsis thaliana and durum wheat during the seed germination stage under NaCl stress conditions. Results indicated that inoculation of both plant spp. with the bacterial strains separately or combined considerably improved the growth parameters. Three soils with different salinity levels (S1 = 0.48, S2 = 3.81, and S3 = 2.80 mS/cm) were used to investigate the effects of selected strains (BR5, OR15, and RB13; separately and combined) on several growth parameters of wheat plants. The inoculation (notably the multi-strain consortium) proved a better approach to increase the chlorophyll and carotenoid contents as compared to control plants. However, proline content, lipid peroxidation, and activities of antioxidant enzymes decreased after inoculation with the plant growth-promoting rhizobacteria (PGPR) that can attenuate the adverse effects of salt stress by reducing the reactive oxygen species (ROS) production. These results indicated that under saline soil conditions, halotolerant PGPR strains are promising candidates as biofertilizers under salt stress conditions.
Souhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences 2021, 11, 1034 .
AMA StyleSouhila Kerbab, Allaoua Silini, Ali Chenari Bouket, Hafsa Cherif-Silini, Manal Eshelli, Nour El Houda Rabhi, Lassaad Belbahri. Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria. Applied Sciences. 2021; 11 (3):1034.
Chicago/Turabian StyleSouhila Kerbab; Allaoua Silini; Ali Chenari Bouket; Hafsa Cherif-Silini; Manal Eshelli; Nour El Houda Rabhi; Lassaad Belbahri. 2021. "Mitigation of NaCl Stress in Wheat by Rhizosphere Engineering Using Salt Habitat Adapted PGPR Halotolerant Bacteria." Applied Sciences 11, no. 3: 1034.
The phosphogypsum (PG) endogenous bacterial community and endophytic bacterial communities of four plants growing in phosphogypsum-contaminated sites, Suaeda fruticosa (SF), Suaeda mollis (SM), Mesembryanthmum nodiflorum (MN) and Arthrocnemum indicum (AI) were investigated by amplicon sequencing. Results highlight a more diverse community of phosphogypsum than plants associated endophytic communities. Additionally, the bacterial culturable communities of phosphogypsum and associated plant endophytes were isolated and their plant-growth promotion capabilities, bioremediation potential and stress tolerance studied. Most of plant endophytes were endowed with plant growth-promoting (PGP) activities and phosphogypsum communities and associated plants endophytes proved highly resistant to salt, metal and antibiotic stress. They also proved very active in bioremediation of phosphogypsum and other organic and inorganic environmental pollutants. Genome sequencing of five members of the phosphogypsum endogenous community showed that they belong to the recently described species Bacillus albus (BA). Genome mining of BA allowed the description of pollutant degradation and stress tolerance mechanisms. Prevalence of this tool box in the core, accessory and unique genome allowed to conclude that accessory and unique genomes are critical for the dynamics of strain acquisition of bioremediation abilities. Additionally, secondary metabolites (SM) active in bioremediation such as petrobactin have been characterized. Taken together, our results reveal hidden untapped valuable bacterial actors for waste remediation.
Fedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms 2019, 7, 382 .
AMA StyleFedia Ben Mefteh, Ali Chenari Bouket, Amal Daoud, Lenka Luptakova, Faizah N. Alenezi, Neji Gharsallah, Lassaad Belbahri, Ben Mefteh. Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation. Microorganisms. 2019; 7 (10):382.
Chicago/Turabian StyleFedia Ben Mefteh; Ali Chenari Bouket; Amal Daoud; Lenka Luptakova; Faizah N. Alenezi; Neji Gharsallah; Lassaad Belbahri; Ben Mefteh. 2019. "Metagenomic Insights and Genomic Analysis of Phosphogypsum and Its Associated Plant Endophytic Microbiomes Reveals Valuable Actors for Waste Bioremediation." Microorganisms 7, no. 10: 382.
In this study, an efficient in vitro procedure was developed for bud induction, rooting of developing shoots and greenhouse acclimatization of young plantlets of dragon tree (Dracaena draco). Effects of media (S1 (1 mg/L KIN and 1 mg/L NAA), S2 (3 mg/L KIN and 1 mg/L IAA), S3 (1 mg/L BAP and 2 mg/L IBA) and S4 (1 mg/L BAP and 1 mg/L NAA)) on shoot induction and media (R1 (0 mg/L IBA), R2 (0.5 mg/L IBA), R3 (1 mg/L IBA), and R4 (2 mg/L IBA)) on root induction were examined in order to find optimal plant hormone concentrations for efficient Dracaena draco dormant bud development and subsequent rooting. The best shoot induction and rooting media were S1 and S2, and R3 and R4, respectively. Dormant buds from one-year-old Dracaena draco plants submitted to this in vitro procedure allowed successful recovery of up to 8 individuals per explant used. In vitro grown plants were successfully acclimated in the greenhouse. The potential of this in vitro procedure for multiplication of this endangered tree is discussed in this report.
Alexis Galus; Ali Chenari Bouket; Lassaad Belbahri. In Vitro Propagation and Acclimatization of Dragon Tree (Dracaena draco). Horticulturae 2019, 5, 64 .
AMA StyleAlexis Galus, Ali Chenari Bouket, Lassaad Belbahri. In Vitro Propagation and Acclimatization of Dragon Tree (Dracaena draco). Horticulturae. 2019; 5 (3):64.
Chicago/Turabian StyleAlexis Galus; Ali Chenari Bouket; Lassaad Belbahri. 2019. "In Vitro Propagation and Acclimatization of Dragon Tree (Dracaena draco)." Horticulturae 5, no. 3: 64.
Oomycete and fungal pathogens, mainly Phytophthora and Fusarium species, are notorious causal agents of huge economic losses and environmental damages. For instance, Phytophthora ramorum, Phytophthora cryptogea, Phytophthora plurivora and Fusarium solani cause significant losses in nurseries and in forest ecosystems. Chemical treatments, while harmful to the environment and human health, have been proved to have little or no impact on these species. Recently, biocontrol bacterial species were used to cope with these pathogens and have shown promising prospects towards sustainable and eco-friendly agricultural practices. Olive trees prone to Phytophthora and Fusarium disease outbreaks are suitable for habitat-adapted symbiotic strategies, to recover oomycetes and fungal pathogen biocontrol agents. Using this strategy, we showed that olive trees-associated microbiome represents a valuable source for microorganisms, promoting plant growth and healthy benefits in addition to being biocontrol agents against oomycete and fungal diseases. Isolation, characterization and screening of root microbiome of olive trees against numerous Phytophthora and other fungal pathogens have led to the identification of the Bacillus velezensis OEE1, with plant growth promotion (PGP) abilities and strong activity against major oomycete and fungal pathogens. Phylogenomic analysis of the strain OEE1 showed that B. velezensis suffers taxonomic imprecision that blurs species delimitation, impacting their biofertilizers’ practical use. Genome mining of several B. velezensis strains available in the GenBank have highlighted a wide array of plant growth promoting rhizobacteria (PGPR) features, metals and antibiotics resistance and the degradation ability of phytotoxic aromatic compounds. Strain OEE1 harbours a large repertoire of secreted and volatile secondary metabolites. Rarefaction analysis of secondary metabolites richness in the B. velezenis genomes, unambiguously documented new secondary metabolites from ongoing genome sequencing efforts that warrants more efforts in order to assess the huge diversity in the species. Comparative genomics indicated that B. velezensis harbours a core genome endowed with PGP features and accessory genome encoding diverse secondary metabolites. Gas Chromatography-Mass Spectrometry (GC-MS) analysis of OEE1 Volatile Organic Compounds (VOCs) and Liquid Chromatography High Resolution Mas Spectrometry (LC-HRMS) analysis of secondary metabolites identified numerous molecules with PGP abilities that are known to interfere with pathogen development. Moreover, B. velezensis OEE1 proved effective in protecting olive trees against F. solani in greenhouse experiments and are able to inhabit olive tree roots. Our strategy provides an effective means for isolation of biocontrol agents against recalcitrant pathogens. Their genomic analysis provides necessary clues towards their efficient implementation as biofertilizers.
Manel Cheffi; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Marta Belka; Armelle Vallat; Mostafa E. Rateb; Slim Tounsi; Mohamed Ali Triki; Lassaad Belbahri. Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes. Microorganisms 2019, 7, 314 .
AMA StyleManel Cheffi, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Marta Belka, Armelle Vallat, Mostafa E. Rateb, Slim Tounsi, Mohamed Ali Triki, Lassaad Belbahri. Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes. Microorganisms. 2019; 7 (9):314.
Chicago/Turabian StyleManel Cheffi; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Marta Belka; Armelle Vallat; Mostafa E. Rateb; Slim Tounsi; Mohamed Ali Triki; Lassaad Belbahri. 2019. "Olea europaea L. Root Endophyte Bacillus velezensis OEE1 Counteracts Oomycete and Fungal Harmful Pathogens and Harbours a Large Repertoire of Secreted and Volatile Metabolites and Beneficial Functional Genes." Microorganisms 7, no. 9: 314.
In the arid region Bou-Saâda at the South of Algeria, durum wheat Triticum durum L. cv Waha production is severely threatened by abiotic stresses, mainly drought and salinity. Plant growth-promoting rhizobacteria (PGPR) hold promising prospects towards sustainable and environmentally-friendly agriculture. Using habitat-adapted symbiosis strategy, the PGPR Pantoea agglomerans strain Pa was recovered from wheat roots sampled in Bou-Saâda, conferred alleviation of salt stress in durum wheat plants and allowed considerable growth in this unhostile environment. Strain Pa showed growth up to 35 °C temperature, 5–10 pH range, and up to 30% polyethylene glycol (PEG), as well as 1 M salt concentration tolerance. Pa strain displayed pertinent plant growth promotion (PGP) features (direct and indirect) such as hormone auxin biosynthesis, production of 1-aminocyclopropane-1-carboxylate (ACC) deaminase, and ammonia and phosphate solubilization. PGPR features were stable over wide salt concentrations (0–400 mM). Pa strain was also able to survive in seeds, in the non-sterile and sterile wheat rhizosphere, and was shown to have an endophytic life style. Phylogenomic analysis of strain Pa indicated that Pantoea genus suffers taxonomic imprecision which blurs species delimitation and may have impacted their practical use as biofertilizers. When applied to plants, strain Pa promoted considerable growth of wheat seedlings, high chlorophyll content, lower accumulation of proline, and favored K+ accumulation in the inoculated plants when compared to Na+ in control non-inoculated plants. Metabolomic profiling of strain Pa under one strain many compounds (OSMAC) conditions revealed a wide diversity of secondary metabolites (SM) with interesting salt stress alleviation and PGP activities. All these findings strongly promote the implementation of Pantoea agglomerans strain Pa as an efficient biofertilizer in wheat plants culture in arid and salinity-impacted regions.
Hafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences 2019, 20, 3989 .
AMA StyleHafsa Cherif-Silini, Bathini Thissera, Ali Chenari Bouket, Nora Saadaoui, Allaoua Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Lenka Luptakova, Bilal Yahiaoui, Semcheddine Cherrad, Sebastien Vacher, Mostafa E. Rateb, Lassaad Belbahri. Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal. International Journal of Molecular Sciences. 2019; 20 (16):3989.
Chicago/Turabian StyleHafsa Cherif-Silini; Bathini Thissera; Ali Chenari Bouket; Nora Saadaoui; Allaoua Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Bilal Yahiaoui; Semcheddine Cherrad; Sebastien Vacher; Mostafa E. Rateb; Lassaad Belbahri. 2019. "Durum Wheat Stress Tolerance Induced by Endophyte Pantoea agglomerans with Genes Contributing to Plant Functions and Secondary Metabolite Arsenal." International Journal of Molecular Sciences 20, no. 16: 3989.
Halophyte Limoniastrum monopetalum, an evergreen shrub inhabiting the Mediterranean region, has well-documented phytoremediation potential for metal removal from polluted sites. It is also considered to be a medicinal halophyte with potent activity against plant pathogens. Therefore, L. monopetalum may be a suitable candidate for isolating endophytic microbiota members that provide plant growth promotion (PGP) and resistance to abiotic stresses. Selected for biocontrol abilities, these endophytes may represent multifaceted and versatile biocontrol agents, combining pathogen biocontrol in addition to PGP and plant protection against abiotic stresses. In this study 117 root culturable bacterial endophytes, including Gram-positive (Bacillus and Brevibacillus), Gram-negative (Proteus, Providencia, Serratia, Pantoea, Klebsiella, Enterobacter and Pectobacterium) and actinomycete Nocardiopsis genera have been recovered from L. monopetalum. The collection exhibited high levels of biocontrol abilities against bacterial (Agrobacterium tumefaciens MAT2 and Pectobacterium carotovorum MAT3) and fungal (Alternaria alternata XSZJY-1, Rhizoctonia bataticola MAT1 and Fusarium oxysporum f. sp. radicis lycopersici FORL) pathogens. Several bacteria also showed PGP capacity and resistance to antibiotics and metals. A highly promising candidate Bacillus licheniformis LMRE 36 with high PGP, biocontrol, metal and antibiotic, resistance was subsequently tested in planta (potato and olive trees) for biocontrol of a collection of 14 highly damaging Fusarium species. LMRE 36 proved very effective against the collection in both species and against an emerging Fusarium sp. threatening olive trees culture in nurseries. These findings provide a demonstration of our pyramiding strategy. Our strategy was effective in combining desirable traits in biocontrol agents towards broad-spectrum resistance against pathogens and protection of crops from abiotic stresses. Stacking multiple desirable traits into a single biocontrol agent is achieved by first, careful selection of a host for endophytic microbiota recovery; second, stringent in vitro selection of candidates from the collection; and third, application of the selected biocontrol agents in planta experiments. That pyramiding strategy could be successfully used to mitigate effects of diverse biotic and abiotic stresses on plant growth and productivity. It is anticipated that the strategy will provide a new generation of biocontrol agents by targeting the microbiota of plants in hostile environments.
Houda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms 2019, 7, 249 .
AMA StyleHouda Ben Slama, Mohamed Ali Triki, Ali Chenari Bouket, Fedia Ben Mefteh, Faizah N. Alenezi, Lenka Luptakova, Hafsa Cherif-Silini, Armelle Vallat, Tomasz Oszako, Neji Gharsallah, Lassaad Belbahri. Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents. Microorganisms. 2019; 7 (8):249.
Chicago/Turabian StyleHouda Ben Slama; Mohamed Ali Triki; Ali Chenari Bouket; Fedia Ben Mefteh; Faizah N. Alenezi; Lenka Luptakova; Hafsa Cherif-Silini; Armelle Vallat; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. 2019. "Screening of the High-Rhizosphere Competent Limoniastrum monopetalum’ Culturable Endophyte Microbiota Allows the Recovery of Multifaceted and Versatile Biocontrol Agents." Microorganisms 7, no. 8: 249.
To explore proteolytic activity of endophytic fungi inhabiting date palm roots, a Penicillium bilaiae isolate, displaying the highest level of protease production, has been recovered. Response surface methodology (RSM) was applied to optimize culture conditions for protease production by the fungus. Plackett-Burman design allowed for screening of variables effective in protease production. Results indicated that temperature, initial pH and glucose concentration dramatically affect protease yield. These factors were further optimized using a Box-Behnken design and RSM. A combination of initial pH (6.26), temperature (24.5 °C), glucose (13.75 g/L), NaNO₃ (1.5 g/L), MgSO₄ (0.2 g/L), KH₂PO₄ (0.5 g/L) and KCl (0.5 g/L) were optimum for maximum production of protease. A 1086-fold enhancement of protease production was gained after optimization. Biochemical properties of fungal protease including the effect of pH and temperature on the activity and the stability of proteolytic enzyme were determined. Moreover, the influence of carbon and nitrogen sources, metal ions, detergents as well as enzyme inhibitors was investigated. Our results highlighted that protease of Penicillium bilaiae isolate TDPEF30 could be considered as a promising candidate for industrial applications.
Fedia Ben Mefteh; Fakher Frikha; Amal Daoud; Ali Chenari Bouket; Lenka Luptakova; Faizah N. Alenezi; Bader S. Al-Anzi; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.). Microorganisms 2019, 7, 74 .
AMA StyleFedia Ben Mefteh, Fakher Frikha, Amal Daoud, Ali Chenari Bouket, Lenka Luptakova, Faizah N. Alenezi, Bader S. Al-Anzi, Tomasz Oszako, Neji Gharsallah, Lassaad Belbahri. Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.). Microorganisms. 2019; 7 (3):74.
Chicago/Turabian StyleFedia Ben Mefteh; Fakher Frikha; Amal Daoud; Ali Chenari Bouket; Lenka Luptakova; Faizah N. Alenezi; Bader S. Al-Anzi; Tomasz Oszako; Neji Gharsallah; Lassaad Belbahri. 2019. "Response Surface Methodology Optimization of an Acidic Protease Produced by Penicillium bilaiae Isolate TDPEF30, a Newly Recovered Endophytic Fungus from Healthy Roots of Date Palm Trees (Phoenix dactylifera L.)." Microorganisms 7, no. 3: 74.
Endophytic fungi of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) represent a promising source of bioactive compounds with biomedical, industrial, and pharmaceutical applications. The fungal endophytes Penicillium citrinum isolate TDPEF34, and Geotrichum candidum isolate TDPEF20 from healthy and BLD date palm trees, respectively, proved very effective in confrontation assays against three pathogenic bacteria, including two Gram-positive bacteria Bacillus thuringiensis (Bt) and Enterococcus faecalis (Ef), and one Gram-negative bacterium Salmonella enterica (St). They also inhibited the growth of three fungi Trichoderma sp. (Ti), Fusarium sporotrichioides (Fs), Trichoderma sp. (Ts). Additionally, their volatile organic compounds (VOCs) were shown to be in part responsible for the inhibition of Ti and Ts and could account for the full inhibition of Fs. Therefore, we have explored their potential as fungal cell factories for bioactive metabolites production. Four extracts of each endophyte were prepared using different solvent polarities, ethanol (EtOH), ethyl acetate (EtOAc), hexane (Hex), and methanol (MetOH). Both endophyte species showed varying degrees of inhibition of the bacterial and fungal pathogens according to the solvent used. These results suggest a good relationship between fungal bioactivities and their produced secondary metabolites. Targeting the discovery of potential anti-diabetic, anti-hemolysis, anti-inflammatory, anti-obesity, and cytotoxic activities, endophytic extracts showed promising results. The EtOAc extract of G. candidum displayed IC50 value comparable to the positive control diclofenac sodium in the anti-inflammatory assays. Antioxidant activity was evaluated using α,α-diphenyl-β-picrylhydrazyl (DPPH), β-carotene bleaching, reducing power (RP), and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonique) (ABTS) radical scavenging assays. The findings revealed strong anti-oxidant power with an IC50 of 177.55 µg/mL for G. candidum EtOAc extract using DPPH assay, probably due to high polyphenol and flavonoid content in both fungal extracts. Finally, LC-HRMS (Liquid Chromatography–High Resolution Mass Spectrometry) and GC-MS (Gas Chromatography–Mass Spectrometry) analysis of G. candidum and P. citrinum extracts revealed an impressive arsenal of compounds with previously reported biological activities, partly explaining the obtained results. Finally, LC-HRMS analysis indicated the presence of new fungal metabolites that have never been reported, which represent good candidates to follow for the discovery of new bioactive molecules.
Fedia Ben Mefteh; Amal Daoud; Ali Chenari Bouket; Bathini Thissera; Yamina Kadri; Hafsa Cherif-Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Tomasz Oszako; Adel Kadri; José María Ros-García; Mostafa E. Rateb; Neji Gharsallah; Lassaad Belbahri. Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites. International Journal of Molecular Sciences 2018, 19, 1986 .
AMA StyleFedia Ben Mefteh, Amal Daoud, Ali Chenari Bouket, Bathini Thissera, Yamina Kadri, Hafsa Cherif-Silini, Manal Eshelli, Faizah N. Alenezi, Armelle Vallat, Tomasz Oszako, Adel Kadri, José María Ros-García, Mostafa E. Rateb, Neji Gharsallah, Lassaad Belbahri. Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites. International Journal of Molecular Sciences. 2018; 19 (7):1986.
Chicago/Turabian StyleFedia Ben Mefteh; Amal Daoud; Ali Chenari Bouket; Bathini Thissera; Yamina Kadri; Hafsa Cherif-Silini; Manal Eshelli; Faizah N. Alenezi; Armelle Vallat; Tomasz Oszako; Adel Kadri; José María Ros-García; Mostafa E. Rateb; Neji Gharsallah; Lassaad Belbahri. 2018. "Date Palm Trees Root-Derived Endophytes as Fungal Cell Factories for Diverse Bioactive Metabolites." International Journal of Molecular Sciences 19, no. 7: 1986.
The Gram positive, non-pathogenic endospore-forming soil inhabiting prokaryote Bacillus amyloliquefaciens is a plant growth-promoting rhizobacterium. Bacillus amyloliquefaciens processes wide biocontrol abilities and numerous strains have been reported to suppress diverse bacterial, fungal and fungal-like pathogens. Knowledge about strain level biocontrol abilities is warranted to translate this knowledge into developing more efficient biocontrol agents and bio-fertilizers. Ever-expanding genome studies of B. amyloliquefaciens are showing tremendous increase in strain-specific new secondary metabolite clusters which play key roles in the suppression of pathogens and plant growth promotion. In this report, we have used genome mining of all sequenced B. amyloliquefaciens genomes to highlight species boundaries, the diverse strategies used by different strains to promote plant growth and the diversity of their secondary metabolites. Genome composition of the targeted strains suggest regions of genomic plasticity that shape the structure and function of these genomes and govern strain adaptation to different niches. Our results indicated that B. amyloliquefaciens: i) suffer taxonomic imprecision that blurs the debate over inter-strain genome diversity and dynamics, ii) have diverse strategies to promote plant growth and development, iii) have an unlocked, yet to be delimited impressive arsenal of secondary metabolites and products, iv) have large number of so-called orphan gene clusters, i.e. biosynthetic clusters for which the corresponding metabolites are yet unknown, and v) have a dynamic pan genome with a secondary metabolite rich accessory genome.
Lassaad Belbahri; Ali Chenari Bouket; Imen Rekik; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Eva Petrovova; Tomasz Oszako; Semcheddine Cherrad; Sébastien Vacher; Mostafa E. Rateb. Comparative Genomics of Bacillus amyloliquefaciens Strains Reveals a Core Genome with Traits for Habitat Adaptation and a Secondary Metabolites Rich Accessory Genome. Frontiers in Microbiology 2017, 8, 1438 .
AMA StyleLassaad Belbahri, Ali Chenari Bouket, Imen Rekik, Faizah N. Alenezi, Armelle Vallat, Lenka Luptakova, Eva Petrovova, Tomasz Oszako, Semcheddine Cherrad, Sébastien Vacher, Mostafa E. Rateb. Comparative Genomics of Bacillus amyloliquefaciens Strains Reveals a Core Genome with Traits for Habitat Adaptation and a Secondary Metabolites Rich Accessory Genome. Frontiers in Microbiology. 2017; 8 ():1438.
Chicago/Turabian StyleLassaad Belbahri; Ali Chenari Bouket; Imen Rekik; Faizah N. Alenezi; Armelle Vallat; Lenka Luptakova; Eva Petrovova; Tomasz Oszako; Semcheddine Cherrad; Sébastien Vacher; Mostafa E. Rateb. 2017. "Comparative Genomics of Bacillus amyloliquefaciens Strains Reveals a Core Genome with Traits for Habitat Adaptation and a Secondary Metabolites Rich Accessory Genome." Frontiers in Microbiology 8, no. : 1438.
The soil-borne gram-positive bacteria Aneurinibacillus migulanus strain Nagano shows considerable potential as a biocontrol agent against plant diseases. In contrast, A. migulanus NCTC 7096 proved less effective for inhibition of plant pathogens. Nagano strain exerts biocontrol activity against some gram-positive and gram-negative bacteria, fungi and oomycetes through the production of gramicidin S (GS). Apart from the antibiotic effects, GS increases the rate of evaporation from the plant surface, reducing periods of surface wetness and thereby indirectly inhibiting spore germination. To elucidate the molecular basis of differential biocontrol abilities of Nagano and NCTC 7096, we compared GS production and biosurfactant secretion in addition to genome mining of the genomes. Our results proved that: (i) Using oil spreading, blood agar lysis, surface tension and tomato leaves wetness assays, Nagano showed increased biosurfactant secretion in comparison with NCTC 7096, (ii) Genome mining indicated the presence of GS genes in both Nagano and NCTC 7096 with two amino acid units difference between the strains: T342I and P419S. Using 3D models and the DUET server, T342I and P419S were predicted to decrease the stability of the NCTC 7096 GS synthase, (iii) Nagano produced two additional GS-like molecules GS-1155 (molecular weight 1155) and GS-1169 (molecular weight 1169), where one or two ornithine residues replace lysine in the peptide. There was also a negative correlation between surface tension and the quantity of GS-1169 present in Nagano, and (iv) the Nagano genome had a full protein network of exopolysaccharide biosynthesis in contrast to NCTC 7096 which lacked the first enzyme of the network. NCTC 7096 is unable to form biofilms as observed for Nagano. Different molecular layers, mainly gramicidin secondary metabolite production, account for differential biocontrol abilities of Nagano and NCTC 7096. This work highlighted the basis of differential biological control abilities between strains belonging to the same species and demonstrates techniques useful to the screening of effective biocontrol strains for environmentally friendly secondary metabolites that can be used to manage plant pathogens in the field.
Faizah N. Alenezi; Imen Rekik; Ali Chenari Bouket; Lenka Luptakova; Hedda J. Weitz; Mostafa E. Rateb; Marcel Jaspars; Stephen Woodward; Lassaad Belbahri. Increased Biological Activity of Aneurinibacillus migulanus Strains Correlates with the Production of New Gramicidin Secondary Metabolites. Frontiers in Microbiology 2017, 8, 517 .
AMA StyleFaizah N. Alenezi, Imen Rekik, Ali Chenari Bouket, Lenka Luptakova, Hedda J. Weitz, Mostafa E. Rateb, Marcel Jaspars, Stephen Woodward, Lassaad Belbahri. Increased Biological Activity of Aneurinibacillus migulanus Strains Correlates with the Production of New Gramicidin Secondary Metabolites. Frontiers in Microbiology. 2017; 8 ():517.
Chicago/Turabian StyleFaizah N. Alenezi; Imen Rekik; Ali Chenari Bouket; Lenka Luptakova; Hedda J. Weitz; Mostafa E. Rateb; Marcel Jaspars; Stephen Woodward; Lassaad Belbahri. 2017. "Increased Biological Activity of Aneurinibacillus migulanus Strains Correlates with the Production of New Gramicidin Secondary Metabolites." Frontiers in Microbiology 8, no. : 517.
Wastewater reuse in agriculture may help mitigate water scarcity. This may be reached if high quality treatments removing harmful pollutants are applied. The aim of the present study was to compare the effect of untreated (UTW) and treated wastewater (TW) on germination and seedlings development of alfalfa (Medicago sativa L.), fescue (Festuca arundinacea Schreb.) and sorghum (Sorghum bicolor (L.) Moench). UTW presented high turbidity (130 NTU), chemical and biological oxygen demand (COD, 719mgL, BOD 291mgL) and metal concentrations. These levels caused mortality (18% for fescue), decreased germination speed in seeds (37.5% for alfalfa) and reductions of root and stem length in seedlings (80% and 22% respectively for alfalfa). Adverse effects on seeds germination were reflected at the biochemical level by increased HO levels (6 times for sorghum after 5days) and by increased Malondialdehyde (MDA) levels (more than 600 times for sorghum roots) during seedlings development. When TW was used, these parameters were close to control seeds ones. They were also dependent on plant species and developmental stage. Therefore, for efficient reclaimed wastewater reuse in irrigation, suitable crops, displaying wide tolerance to toxic contents during germination and later seedling development stages have to be selected.
Imen Rekik; Zayneb Chaabane; Amara Missaoui; Ali Chenari Bouket; Lenka Luptakova; Amine Elleuch; Lassaad Belbahri. Effects of untreated and treated wastewater at the morphological, physiological and biochemical levels on seed germination and development of sorghum ( Sorghum bicolor (L.) Moench), alfalfa ( Medicago sativa L.) and fescue ( Festuca arundinacea Schreb.). Journal of Hazardous Materials 2017, 326, 165 -176.
AMA StyleImen Rekik, Zayneb Chaabane, Amara Missaoui, Ali Chenari Bouket, Lenka Luptakova, Amine Elleuch, Lassaad Belbahri. Effects of untreated and treated wastewater at the morphological, physiological and biochemical levels on seed germination and development of sorghum ( Sorghum bicolor (L.) Moench), alfalfa ( Medicago sativa L.) and fescue ( Festuca arundinacea Schreb.). Journal of Hazardous Materials. 2017; 326 ():165-176.
Chicago/Turabian StyleImen Rekik; Zayneb Chaabane; Amara Missaoui; Ali Chenari Bouket; Lenka Luptakova; Amine Elleuch; Lassaad Belbahri. 2017. "Effects of untreated and treated wastewater at the morphological, physiological and biochemical levels on seed germination and development of sorghum ( Sorghum bicolor (L.) Moench), alfalfa ( Medicago sativa L.) and fescue ( Festuca arundinacea Schreb.)." Journal of Hazardous Materials 326, no. : 165-176.
In this study, we aimed to explore and compare the composition, metabolic diversity and antimicrobial potential of endophytic fungi colonizing internal tissues of healthy and brittle leaf diseased (BLD) date palm trees (Phoenix dactylifera L.) widely cultivated in arid zones of Tunisia. A total of 52 endophytic fungi were isolated from healthy and BLD roots of date palm trees, identified based on ITS-rDNA sequence analysis and shown to represent 13 species belonging to five genera. About 36.8% of isolates were shared between healthy and diseased root fungal microbiomes, whereas 18.4% and 44.7% of isolates were specific to healthy and BLD root fungal microbiomes, respectively. All isolates were able to produce at least two of the screened enzymes including amylase, cellulase, chitinase, pectinase, protease, laccase and lipase. A preliminary screening of the isolates using disc diffusion method for antibacterial activity against four Gram-positive and three Gram-negative bacteria and antifungal activities against three phytopathogenic fungi indicated that healthy and BLD root fungal microbiomes displayed interesting bioactivities against examined bacteria and broad spectrum bioactivity against fungal pathogens. Some of these endophytic fungi (17 isolates) were fermented and their extracts were evaluated for antimicrobial potential against bacterial and fungal isolates. Results revealed that fungal extracts exhibited antibacterial activities and were responsible for approximately half of antifungal activities against living fungi. These results suggest a strong link between fungal bioactivities and their secondary metabolite arsenal. EtOAc extracts of Geotrichum candidum and Thielaviopsis punctulata originating from BLD microbiome gave best results against Micrococcus luteus and Bacillus subtilis with minimum inhibitory concentration (MIC, 0.78 mg/mL) and minimum bactericidal concentration (MBC, 6.25 mg/mL). Geotrichum candidum gave the best result against Rhizoctonia solani with MIC 0.78 mg/mL and minimum fungicidal concentration (MFC, 6.25 mg/mL). In conclusion, using plant microbiomes subjected to biotic stresses offers new endophytes with different bioactivities than those of healthy plants. Therefore, date palm endophytic fungi represent a hidden untapped arsenal of antibacterial and broad spectrum antifungal secondary metabolites and could be considered promising source of bioactive compounds with industrial and pharmaceutical applications.
Fedia B. Mefteh; Amal Daoud; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Mostafa E. Rateb; Adel Kadri; Neji Gharsallah; Lassaad Belbahri. Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites. Frontiers in Microbiology 2017, 8, 307 .
AMA StyleFedia B. Mefteh, Amal Daoud, Ali Chenari Bouket, Faizah N. Alenezi, Lenka Luptakova, Mostafa E. Rateb, Adel Kadri, Neji Gharsallah, Lassaad Belbahri. Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites. Frontiers in Microbiology. 2017; 8 ():307.
Chicago/Turabian StyleFedia B. Mefteh; Amal Daoud; Ali Chenari Bouket; Faizah N. Alenezi; Lenka Luptakova; Mostafa E. Rateb; Adel Kadri; Neji Gharsallah; Lassaad Belbahri. 2017. "Fungal Root Microbiome from Healthy and Brittle Leaf Diseased Date Palm Trees (Phoenix dactylifera L.) Reveals a Hidden Untapped Arsenal of Antibacterial and Broad Spectrum Antifungal Secondary Metabolites." Frontiers in Microbiology 8, no. : 307.
Ali Chenari Bouket; Asadollah Babai-Ahari; Mahdi Arzanlou; Motoaki Tojo. Morphological and molecular characterization of Phytopythium litorale and Pp. oedochilum from Iran. Nova Hedwigia 2016, 102, 257 -270.
AMA StyleAli Chenari Bouket, Asadollah Babai-Ahari, Mahdi Arzanlou, Motoaki Tojo. Morphological and molecular characterization of Phytopythium litorale and Pp. oedochilum from Iran. Nova Hedwigia. 2016; 102 (1-2):257-270.
Chicago/Turabian StyleAli Chenari Bouket; Asadollah Babai-Ahari; Mahdi Arzanlou; Motoaki Tojo. 2016. "Morphological and molecular characterization of Phytopythium litorale and Pp. oedochilum from Iran." Nova Hedwigia 102, no. 1-2: 257-270.
Pythium kandovanense sp. nov. (ex-type culture CCTU 1813T = OPU 1626T = CBS 139567T) is a novel oomycete species isolated from Lolium perenne with snow rot symptoms in a natural grassland in East-Azarbaijan province, Iran. Phylogenetic analyses based on sequence data from internal transcribed spacer (ITS)-rDNA, coxI and coxII mitochondrial genes clustered our isolates in Pythium group E as a unique, well supported clade. Pythium kandovanense sp. nov. is phylogenetically and morphologically distinct from the other closely related species in this clade, namely Pythium rostratifingens and Pythium rostratum. Pythium kandovanense sp. nov. can be distinguished from these two species by its cylindrical sporangia and lower temperatures for optimum and maximum growth rate. The development of zoospores released through a shorter discharge tube is an additional morphological feature which can be used to differentiate Pythium kandovanense sp. nov. from Pythium rostratifingens. Laboratory inoculation tests demonstrated the pathogenicity of Pythium kandovanense sp. nov. to L. perenne under wet cold (0–3 °C) conditions.
Ali Chenari Bouket; Mahdi Arzanlou; Motoaki Tojo; Asadollah Babai-Ahari. Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves. International Journal of Systematic and Evolutionary Microbiology 2015, 65, 2500 -2506.
AMA StyleAli Chenari Bouket, Mahdi Arzanlou, Motoaki Tojo, Asadollah Babai-Ahari. Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves. International Journal of Systematic and Evolutionary Microbiology. 2015; 65 (8):2500-2506.
Chicago/Turabian StyleAli Chenari Bouket; Mahdi Arzanlou; Motoaki Tojo; Asadollah Babai-Ahari. 2015. "Pythium kandovanense sp. nov., a fungus-like eukaryotic micro-organism (Stramenopila, Pythiales) isolated from snow-covered ryegrass leaves." International Journal of Systematic and Evolutionary Microbiology 65, no. 8: 2500-2506.
Ali Chenari Bouket. Hierarchical cluster analysis of Criconemoides species (Nematoda: Criconematidae), with a proposal for unknown species identification. Archives Of Phytopathology And Plant Protection 2013, 47, 90 -105.
AMA StyleAli Chenari Bouket. Hierarchical cluster analysis of Criconemoides species (Nematoda: Criconematidae), with a proposal for unknown species identification. Archives Of Phytopathology And Plant Protection. 2013; 47 (1):90-105.
Chicago/Turabian StyleAli Chenari Bouket. 2013. "Hierarchical cluster analysis of Criconemoides species (Nematoda: Criconematidae), with a proposal for unknown species identification." Archives Of Phytopathology And Plant Protection 47, no. 1: 90-105.