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Phosphorus (P) is an important nutrient in plant nutrition. Its absorption by plants from the soil is influenced by many factors. Therefore, a foliar application of this nutrient could be utilized for the optimal nutrition state of plants. The premise of the study is that foliar application of phosphorus will increase the yield of normal-phytate (npa) cultivars (CDC Bronco a Cutlass) and low-phytate (lpa) lines (1-2347-144, 1-150-81) grown in soils with low phosphorus supply and affect seed quality depending on the ability of the pea to produce phytate. A graded application of phosphorus (H₃PO₄) in four doses: without P (P0), 27.3 mg P (P1), 54.5 mg P (P2), and 81.8 mg P/pot (P3) realized at the development stages of the 6th true leaf led to a significant increase of chlorophyll contents, and fluorescence parameters of chlorophyll expressing the CO2 assimilation velocity. The P fertilization increased the yield of seeds significantly, except the highest dose of phosphorus (P3) at which the yield of the npa cultivars was reduced. The line 1-2347-144 was the most sensible to the P application when the dose P3 increased the seed production by 42.1%. Only the lpa line 1-150-81 showed a decreased tendency in the phytate content at the stepped application of the P nutrition. Foliar application of phosphorus significantly increased ash material in seed, but did not tend to affect the protein and mineral content of seeds. Only the zinc content in seeds was significantly reduced by foliar application of P in npa and lpa pea genotypes. It is concluded from the present study that foliar phosphorus application could be an effective way to enhance the pea growth in P-deficient condition with a direct effect on seed yield and quality.
Petr Škarpa; Marie Školníková; Jiří Antošovský; Pavel Horký; Iva Smýkalová; Jiří Horáček; Radmila Dostálová; Zdenka Kozáková. Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization. Plants 2021, 10, 1608 .
AMA StylePetr Škarpa, Marie Školníková, Jiří Antošovský, Pavel Horký, Iva Smýkalová, Jiří Horáček, Radmila Dostálová, Zdenka Kozáková. Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization. Plants. 2021; 10 (8):1608.
Chicago/Turabian StylePetr Škarpa; Marie Školníková; Jiří Antošovský; Pavel Horký; Iva Smýkalová; Jiří Horáček; Radmila Dostálová; Zdenka Kozáková. 2021. "Response of Normal and Low-Phytate Genotypes of Pea (Pisum sativum L.) on Phosphorus Foliar Fertilization." Plants 10, no. 8: 1608.
The aim of this publication is to compile a summary of the findings regarding punicalagin in various tissues described thus far in the literature, with an emphasis on the effect of this substance on immune reactions. Punicalagin (PUN) is an ellagitannin found in the peel of pomegranate (Punica granatum). It is a polyphenol with proven antioxidant, hepatoprotective, anti-atherosclerotic and chemopreventive activities, antiproliferative activity against tumor cells; it inhibits inflammatory pathways and the action of toxic substances, and is highly tolerated. This work describes the source, metabolism, functions and effects of punicalagin, its derivatives and metabolites. Furthermore, its anti-inflammatory and antioxidant effects are described.
Eva Venusova; Adriana Kolesarova; Pavel Horky; Petr Slama. Physiological and Immune Functions of Punicalagin. Nutrients 2021, 13, 2150 .
AMA StyleEva Venusova, Adriana Kolesarova, Pavel Horky, Petr Slama. Physiological and Immune Functions of Punicalagin. Nutrients. 2021; 13 (7):2150.
Chicago/Turabian StyleEva Venusova; Adriana Kolesarova; Pavel Horky; Petr Slama. 2021. "Physiological and Immune Functions of Punicalagin." Nutrients 13, no. 7: 2150.
Milk thistle seeds contain a mixture of flavonoids known as silymarin, which consists of silybin, isosilybin, silychristine, and silydianin. Until now, there has been no evidence of monitoring the digestibility of silymarin complex in horses. The aim of the research was to evaluate the digestibility of silymarin complex and the effect of nutrient digestibility in horses. Different daily feed doses (FD) of milk thistle expeller (0 g, 100 g, 200 g, 400 g, 700 g) were administered to five mares kept under the same conditions and at the same feed rations. Digestibility of silymarin complex was monitored by HPLC-UV. Digestible energy (DE), crude protein, crude fat, crude fiber, nitrogen-free extract (NFE), crude ash, calcium (Ca), and phosphorus (P) were determined according ISO/IEC 17025:2017. The biochemical profile of blood plasma (total protein, albumin, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), gamma-glutamyl transferase (GGT), bilirubin, total cholesterol, HDL cholesterol, LDL cholesterol, triacyl glyceride (TAG), non-esterified fatty acid (NEFA), creatine kinase (CK), creatinine, urea, glutathione peroxidase (GSH-Px), total antioxidant status (TAS), glucose, calcium, and inorganic phosphate) was investigated. Moreover, the flavonolignans of the silymarin complex in plasma were detected. Statistically significant differences (p ≤ 0.05) were found between daily doses of milk thistle expellers in digestibilities. Our findings showed the digestibility of flavonolignans increased with the daily dose and then stagnated with the dose of milk thistle seed cakes at 700 g/day.
Hana Dockalova; Ladislav Zeman; Daria Baholet; Andrej Batik; Sylvie Skalickova; Pavel Horky. Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses. Animals 2021, 11, 1687 .
AMA StyleHana Dockalova, Ladislav Zeman, Daria Baholet, Andrej Batik, Sylvie Skalickova, Pavel Horky. Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses. Animals. 2021; 11 (6):1687.
Chicago/Turabian StyleHana Dockalova; Ladislav Zeman; Daria Baholet; Andrej Batik; Sylvie Skalickova; Pavel Horky. 2021. "Dose Effect of Milk Thistle (Silybum marianum) Seed Cakes on the Digestibility of Nutrients, Flavonolignans and the Individual Components of the Silymarin Complex in Horses." Animals 11, no. 6: 1687.
Selenium nanoparticles (SeNPs) are fast becoming a key instrument in several applications such as medicine or nutrition. Questions have been raised about the safety of their use. Male rats were fed for 28 days on a monodiet containing 0.5, 1.5, 3.0 and 5.0 mg Se/kg. Se content in blood and liver, liver panel tests, blood glucose, total antioxidant capacity (TAC), the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPx) were analysed. Liver and duodenum were subjected to histopathology examination. The weight gain of rats showed no differences between tested groups. Se content in blood was higher in all treated groups compared to the control group. The liver concentration of Se in the treated groups varied in the range from 222 to 238 ng/g. No differences were observed in the activity of AST (aspartate aminotransferase), ALP (alkaline phosphatase) and TAS (total antioxidant status). A significant decrease in ALT activity compared to the control group was observed in the treated groups. GPx activity varied from 80 to 88 U/mL through tested groups. SOD activity in liver was decreased in the SeNP-treated group with 5 mg Se/kg (929 ± 103 U/mL). Histopathological examination showed damage to the liver parenchyma and intestinal epithelium in a dose-dependent manner. This study suggests that short-term SeNP supplementation can be safe and beneficial in Se deficiency or specific treatment.
Lenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics 2021, 9, 28 .
AMA StyleLenka Urbankova, Sylvie Skalickova, Magdalena Pribilova, Andrea Ridoskova, Pavlina Pelcova, Jiri Skladanka, Pavel Horky. Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats. Toxics. 2021; 9 (2):28.
Chicago/Turabian StyleLenka Urbankova; Sylvie Skalickova; Magdalena Pribilova; Andrea Ridoskova; Pavlina Pelcova; Jiri Skladanka; Pavel Horky. 2021. "Effects of Sub-Lethal Doses of Selenium Nanoparticles on the Health Status of Rats." Toxics 9, no. 2: 28.
Veterinarians can recommend milk thistle for the treatment of equine liver disease and laminitis. Milk thistle seed cakes were fed in the range of normal feed doses in this study. The milk thistle seed cakes were fed (twice a day) to the experimental group of the horses (n = 5) and biochemical blood markers (TP, Albumin, ALT (alanine transaminase), AST (aspartate transaminase), ALP (alkaline phosphatase), GGT (gamma-glutamyltransferase), Bilirubin, Cholesterol, HDL (high-density lipoprotein), LDL (low-density lipoprotein), TAG (triacylglycerol), BHB (beta-hydroxybutyric acid), NEFA (non-esterified fatty acids), creatine kinase, creatinine, Urea, GSH-Px (glutathione peroxidase), TAS (total antioxidant status), lactate, glucose, cortisol, Ca, Pi) were monitored. The control group of horses (n = 5), bred and trained in the same conditions, was used for comparison. The control group received the entire feed dose as accepted by the horses in the experimental group before the beginning of the experiment. The aim was to find out whether the preparation of milk thistle seed cakes could have positive effects on the health of the horses. All ten horses received one feeding form before the beginning of the experimental monitoring. All horses were exposed to heavy physical exercise (regular combined driving training) after 56 days of milk thistle seed cakes feeding (up to 400 g/day). Three blood samples were taken (before physical exercise; about 15 min and 60 min after physical exercise). Significant differences (p < 0.05) were detected in the values of AST, NEFA, cortisol and Pi in the experimental group. The exercise effect was detected in the values of albumin, lactate, cortisol, NEFA, and calcium. Our results suggest that the feeding of milk thistle seed cakes could have a positive effect on the health of the horses.
Hana Dockalova; Ladislav Zeman; Pavel Horky. Influence of Milk Thistle (Silybum marianum) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion. Animals 2021, 11, 210 .
AMA StyleHana Dockalova, Ladislav Zeman, Pavel Horky. Influence of Milk Thistle (Silybum marianum) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion. Animals. 2021; 11 (1):210.
Chicago/Turabian StyleHana Dockalova; Ladislav Zeman; Pavel Horky. 2021. "Influence of Milk Thistle (Silybum marianum) Seed Cakes on Biochemical Values of Equine Plasma Subjected to Physical Exertion." Animals 11, no. 1: 210.
Polysaccharide nanoparticles are promising materials in the wide range of disciplines such as medicine, nutrition, food production, agriculture, material science and others. They excel not only in their non‐toxicity and biodegradability but also in their easy preparation. As well as inorganic particles, a protein corona (PC) around polysaccharide nanoparticles is formed in biofluids. Moreover, it has been considered that the overall response of the organism to nanoparticles presence depends on the PC. This review summarises scientific publications about the structural chemistry of polysaccharide nanoparticles and their impact on theranostic applications. Three strategies of implementation of the PC in theranostics have been discussed: I) Utilisation of the PC in therapy; II) How the composition of the PC is analysed for specific disease markers; III) How the formed PC can interact with the immune system and enhances the immunomodulation or immunoelimination. Thus, the findings from this review can contribute to improve the design of drug delivery systems. However, it is still necessary to elucidate the mechanisms of nano‐bio interactions and discover new connections in nanoscale research.
Sylvie Skalickova; Pavel Horky; Veronika Mlejnkova; Jiri Skladanka; Bozena Hosnedlova; Branislav Ruttkay‐Nedecky; Carlos Fernandez; Rene Kizek. Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles. The Chemical Record 2020, 21, 17 -28.
AMA StyleSylvie Skalickova, Pavel Horky, Veronika Mlejnkova, Jiri Skladanka, Bozena Hosnedlova, Branislav Ruttkay‐Nedecky, Carlos Fernandez, Rene Kizek. Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles. The Chemical Record. 2020; 21 (1):17-28.
Chicago/Turabian StyleSylvie Skalickova; Pavel Horky; Veronika Mlejnkova; Jiri Skladanka; Bozena Hosnedlova; Branislav Ruttkay‐Nedecky; Carlos Fernandez; Rene Kizek. 2020. "Theranostic Approach for the Protein Corona of Polysaccharide Nanoparticles." The Chemical Record 21, no. 1: 17-28.
Mycotoxin management in agriculture is an essential challenge for maintaining the health of both animals and humans. Choosing the right adsorbent is still a question for many breeders and an important criterion for feed manufacturers. New adsorbents are still being sought. Graphene oxide is a promising material in the field of nanotechnology, which excels in its adsorption properties. Presented in vitro study investigates graphene oxide for the binding of mycotoxins from crushed wheat. The results show that graphene oxide has an adsorption capacity for aflatoxin 0.045 mg/g, zearalenone 0.53 mg/g and deoxynivalenol 1.69 mg/g at 37° C. In vitro simulation of crushed wheat digestion showed rapid adsorption during the gastric phase. Of the minerals, Mg, Cu and Zn were the most adsorbed. The applied dose of graphene oxide of 10 mg/g caused only a slight inhibition of the digestive enzymes α-amylase and trypsin compared to pepsin and gastric lipase. In vitro results indicated the suitability of graphene oxide in the adsorption of the aflatoxin, zearalenone and deoxynivalenol.
Pavel Horky; Eva Venusova; Tereza Aulichova; Andrea Ridoskova; Jiri Skladanka; Sylvie Skalickova. Usability of graphene oxide as a mycotoxin binder: In vitro study. PLOS ONE 2020, 15, e0239479 .
AMA StylePavel Horky, Eva Venusova, Tereza Aulichova, Andrea Ridoskova, Jiri Skladanka, Sylvie Skalickova. Usability of graphene oxide as a mycotoxin binder: In vitro study. PLOS ONE. 2020; 15 (9):e0239479.
Chicago/Turabian StylePavel Horky; Eva Venusova; Tereza Aulichova; Andrea Ridoskova; Jiri Skladanka; Sylvie Skalickova. 2020. "Usability of graphene oxide as a mycotoxin binder: In vitro study." PLOS ONE 15, no. 9: e0239479.
Ivana Koláčková; Daria Baholet; Hana Abigail Gruberová; Vladimír Smutný; Petr Elzner; Pavel Horký; Jiří Skládanka; Michal Rábek. Effect of Variety, Sowing Date and Location on Yield and Nutritional Characteristics of Sorghum. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2020, 68, 529 -537.
AMA StyleIvana Koláčková, Daria Baholet, Hana Abigail Gruberová, Vladimír Smutný, Petr Elzner, Pavel Horký, Jiří Skládanka, Michal Rábek. Effect of Variety, Sowing Date and Location on Yield and Nutritional Characteristics of Sorghum. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2020; 68 (3):529-537.
Chicago/Turabian StyleIvana Koláčková; Daria Baholet; Hana Abigail Gruberová; Vladimír Smutný; Petr Elzner; Pavel Horký; Jiří Skládanka; Michal Rábek. 2020. "Effect of Variety, Sowing Date and Location on Yield and Nutritional Characteristics of Sorghum." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 68, no. 3: 529-537.
Background The high doses of zinc oxide (ZnO) administered orally to piglets for the prevention of diarrhea and increase of growth rate can contaminate pig farms and the surrounding environment. Therefore, there is a need to find a replacement of high doses of dietary ZnO with an equally effective alternative. In the present study, the effect of two formulations of zinc phosphate-based nanoparticles (ZnA and ZnC NPs) on growth performance, intestinal microbiota, antioxidant status, and intestinal and liver morphology was evaluated. A total of 100 weaned piglets were randomly divided into 10 equal groups with the base diet (control) or the base diet supplemented with ZnA, ZnC, or ZnO at concentrations 500, 1000, and 2000 mg Zn per kilogram of diet. Supplements were given to animals for 10 days. Fecal samples were collected on day 0, 5, 10 and 20. At the end of the treatment (day 10), three piglets from each group were sacrificed and analyzed. Results Comparing to that of control, the significantly higher piglet weight gain was observed in all piglet groups fed with ZnA (P < 0.05). Differences in the total aerobic bacteria and coliform counts in piglet feces after NPs supplementation compared to that of control and ZnO groups were also found (P < 0.05). The majority of aerobic culturable bacteria from the feces represented Escherichia (28.57–47.62%), Enterococcus (3.85–35.71%), and Streptococcus (3.70–42.31%) spp. A total of 542 Escherichia coli isolates were screened for the virulence genes STa, STb, Stx2, F4, and F18. The substantial occurrence of E. coli virulence factors was found on day 5, mainly in fimbrillary antigen and thermostable toxins, except for piglets fed by ZnC. Zn treatment decreased Zn blood levels in piglets fed with ZnO and ZnA (500 mg/kg) and increased in ZnC (2000 mg/kg) compared to that of control (P < 0.05). The antioxidant status of piglets was affected only by ZnA. While some changes in the liver and the intestinal morphology of piglets with NPs were observed, none were serious as reflected by the normal health status and increased weigh gain performance. Conclusions Our results indicate that ZnA NPs have a positive effect on the piglet growth performance even at the lowest concentration. The prevalence of E. coli virulence factors was lowest in pigs supplemented with ZnC. Zinc phosphate-based nanoparticles may be an effective alternative to ZnO.
Silvia Kociova; Kristyna Dolezelikova; Pavel Horky; Sylvie Skalickova; Daria Baholet; Lucie Bozdechova; Eva Vaclavkova; Jaroslava Belkova; Pavel Nevrkla; Jiri Skladanka; Tomas Do; Ondrej Zitka; Yazan Haddad; Pavel Kopel; Ludek Zurek; Vojtech Adam; Kristyna Smerkova. Zinc phosphate-based nanoparticles as alternatives to zinc oxide in diet of weaned piglets. Journal of Animal Science and Biotechnology 2020, 11, 1 -16.
AMA StyleSilvia Kociova, Kristyna Dolezelikova, Pavel Horky, Sylvie Skalickova, Daria Baholet, Lucie Bozdechova, Eva Vaclavkova, Jaroslava Belkova, Pavel Nevrkla, Jiri Skladanka, Tomas Do, Ondrej Zitka, Yazan Haddad, Pavel Kopel, Ludek Zurek, Vojtech Adam, Kristyna Smerkova. Zinc phosphate-based nanoparticles as alternatives to zinc oxide in diet of weaned piglets. Journal of Animal Science and Biotechnology. 2020; 11 (1):1-16.
Chicago/Turabian StyleSilvia Kociova; Kristyna Dolezelikova; Pavel Horky; Sylvie Skalickova; Daria Baholet; Lucie Bozdechova; Eva Vaclavkova; Jaroslava Belkova; Pavel Nevrkla; Jiri Skladanka; Tomas Do; Ondrej Zitka; Yazan Haddad; Pavel Kopel; Ludek Zurek; Vojtech Adam; Kristyna Smerkova. 2020. "Zinc phosphate-based nanoparticles as alternatives to zinc oxide in diet of weaned piglets." Journal of Animal Science and Biotechnology 11, no. 1: 1-16.
Ivana Kolackova; Jiri Skladanka; Sylvie Skalickova; Pavel Horky; Natalia Cernei; Zuzana Lackova; Jiri Trinacty; Vojtech Adam. Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep. Revista Brasileira de Zootecnia 2020, 49, 1 .
AMA StyleIvana Kolackova, Jiri Skladanka, Sylvie Skalickova, Pavel Horky, Natalia Cernei, Zuzana Lackova, Jiri Trinacty, Vojtech Adam. Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep. Revista Brasileira de Zootecnia. 2020; 49 ():1.
Chicago/Turabian StyleIvana Kolackova; Jiri Skladanka; Sylvie Skalickova; Pavel Horky; Natalia Cernei; Zuzana Lackova; Jiri Trinacty; Vojtech Adam. 2020. "Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep." Revista Brasileira de Zootecnia 49, no. : 1.
It is generally believed that antibacterial essential oils have the potential to become one of the alternatives in preventing diarrheal diseases of monogastric animals. The disadvantage is their low efficiency per oral due to easy degradation during digestion in the stomach. This study compares the efficacy of chitosan, alginate-chitosan, guar gum-chitosan, xanthan gum-chitosan and pectin-chitosan nanocapsules to the synthesis of pH-responsive biopolymeric nanocapsule for Thymus vulgaris, Rosmarinus officinalis and Syzygium aromaticum essential oils. Using spectrophotometric approach and gas chromatography, release kinetics were determined in pH 3, 5.6 and 7.4. The growth rates of S. aureus and E. coli, as well as minimal inhibition concentration of essential oils were studied. The average encapsulation efficiency was 60%, and the loading efficiency was 70%. The size of the nanocapsules ranged from 100 nm to 500 nm. Results showed that chitosan-guar gum and chitosan-pectin nanocapsules released 30% of essential oils (EOs) at pH 3 and 80% at pH 7.4 during 3 h. Similar release kinetics were confirmed for thymol, eugenol and α-pinene. Minimal inhibition concentrations of Thymus vulgaris and Syzygium aromaticum essential oils ranged from 0.025 to 0.5%. Findings of this study suggest that the suitable pH-responsive nanocapsule for release, low toxicity and antibacterial activity is based on chitosan-guar gum structure.
Sylvie Skalickova; Tereza Aulichova; Eva Venusova; Jiri Skladanka; Pavel Horky. Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils. International Journal of Molecular Sciences 2020, 21, 1799 .
AMA StyleSylvie Skalickova, Tereza Aulichova, Eva Venusova, Jiri Skladanka, Pavel Horky. Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils. International Journal of Molecular Sciences. 2020; 21 (5):1799.
Chicago/Turabian StyleSylvie Skalickova; Tereza Aulichova; Eva Venusova; Jiri Skladanka; Pavel Horky. 2020. "Development of pH-Responsive Biopolymeric Nanocapsule for Antibacterial Essential Oils." International Journal of Molecular Sciences 21, no. 5: 1799.
Natalia Cernei; Pavel Kopel; Pavel Horky; David Hynek; Ondrej Zitka; Vojtech Adam. ISOLATION OF BRANCHED CHAIN AMINOACIDS (VALINE, LEUCINE AND ARGININE) USING MAGHEMITE PARTICLES. NANOCON 2019 Conference Proeedings 2020, 1 .
AMA StyleNatalia Cernei, Pavel Kopel, Pavel Horky, David Hynek, Ondrej Zitka, Vojtech Adam. ISOLATION OF BRANCHED CHAIN AMINOACIDS (VALINE, LEUCINE AND ARGININE) USING MAGHEMITE PARTICLES. NANOCON 2019 Conference Proeedings. 2020; ():1.
Chicago/Turabian StyleNatalia Cernei; Pavel Kopel; Pavel Horky; David Hynek; Ondrej Zitka; Vojtech Adam. 2020. "ISOLATION OF BRANCHED CHAIN AMINOACIDS (VALINE, LEUCINE AND ARGININE) USING MAGHEMITE PARTICLES." NANOCON 2019 Conference Proeedings , no. : 1.
Pavel Kopel; Natalia Cernei; Pavel Horky; Zuzana Lackova; Vedran Milosavljevic; Milica Gagic; Ondrej Zitka; Vojtech Adam. MAGHEMITE PARTICLES FOR SPERMIDINE SEPARATION. NANOCON 2019 Conference Proeedings 2020, 1 .
AMA StylePavel Kopel, Natalia Cernei, Pavel Horky, Zuzana Lackova, Vedran Milosavljevic, Milica Gagic, Ondrej Zitka, Vojtech Adam. MAGHEMITE PARTICLES FOR SPERMIDINE SEPARATION. NANOCON 2019 Conference Proeedings. 2020; ():1.
Chicago/Turabian StylePavel Kopel; Natalia Cernei; Pavel Horky; Zuzana Lackova; Vedran Milosavljevic; Milica Gagic; Ondrej Zitka; Vojtech Adam. 2020. "MAGHEMITE PARTICLES FOR SPERMIDINE SEPARATION." NANOCON 2019 Conference Proeedings , no. : 1.
Essential oils (EOs) are now a hot topic in finding modern substitutes for antibiotics. Many studies have shown positive results and confirmed their high antibacterial activity both in vitro and in vivo. Deservedly, there is an attempt to use EOs as a substitute for antibiotics, which are currently limited by legislation in animal breeding. Given the potential of EOs, studies on their fate in the body need to be summarized. The content of EO's active substances varies depending on growing conditions and consequently on processing and storage. Their content also changes dynamically during the passage through the gastrointestinal tract and their effective concentration can be noticeably diluted at their place of action (small intestine and colon). Based on the solubility of the individual EO's active substances, they are eliminated from the body at different rates. Despite a strong antimicrobial effect, some oils can be toxic to the body and cause damage to the liver, kidneys, or gastrointestinal tissues. Reproductive toxicity has been reported for Origanum vulgare and Mentha arvensis. Several publications also address the effect on the genome. It has been observed that EOs can show both genoprotective effects (Syzygium aromaticum) and genotoxicity, as is the case of Cinnamomum camphor. This review shows that although oils are mainly studied as promising antimicrobials, it is also important to assess animal safety.
Pavel Horky; Sylvie Skalickova; Kristyna Smerkova; Jiri Skladanka. Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals. Animals 2019, 9, 352 .
AMA StylePavel Horky, Sylvie Skalickova, Kristyna Smerkova, Jiri Skladanka. Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals. Animals. 2019; 9 (6):352.
Chicago/Turabian StylePavel Horky; Sylvie Skalickova; Kristyna Smerkova; Jiri Skladanka. 2019. "Essential Oils as a Feed Additives: Pharmacokinetics and Potential Toxicity in Monogastric Animals." Animals 9, no. 6: 352.
Development of new nanomaterials that inhibit or kill bacteria is an important and timely research topic. For example, financial losses due to infectious diseases, such as diarrhea, are a major concern in livestock productions around the world. Antimicrobial nanoparticles (NPs) represent a promising alternative to antibiotics and may lower antibiotic use and consequently spread of antibiotic resistance traits among bacteria, including pathogens. Four formulations of zinc nanoparticles (ZnA, ZnB, ZnC, and ZnD) based on phosphates with spherical (ZnA, ZnB) or irregular (ZnC, ZnD) morphology were prepared. The highest in vitro inhibitory effect of our NPs was observed against Staphylococcus aureus (inhibitory concentration values, IC50, ranged from 0.5 to 1.6 mmol/L), followed by Escherichia coli (IC50 0.8–1.5 mmol/L). In contrast, methicillin resistant S. aureus (IC50 1.2–4.7 mmol/L) was least affected and this was similar to inhibitory patterns of commercial ZnO-based NPs and ZnO. After the successful in vitro testing, the in vivo study with rats based on dietary supplementation with zinc NPs was conducted. Four groups of rats were treated by 2,000 mg Zn/kg diet of ZnA, ZnB, ZnC, and ZnD, for comparison two groups were supplemented by 2,000 mg Zn/kg diet of ZnO-N and ZnO, and one group (control) was fed only by basal diet. The significantly higher (P < 0.05) Zn level in liver and kidney of all treated groups was found, nevertheless Zn NPs did not greatly influence antioxidant status of rats. However, the total aerobic and coliform bacterial population in rat feces significantly decreased (P < 0.05) in all zinc groups after 30 d of the treatment. Furthermore, when compared to the ZnO group, ZnA and ZnC nanoparticles reduced coliforms significantly more (P < 0.05). Our results demonstrate that phosphate-based zinc nanoparticles have the potential to act as antibiotic agents.
Pavel Horky; Sylvie Skalickova; Lenka Urbankova; Daria Baholet; Silvia Kociova; Zuzana Bytešníková; Eliska Kabourkova; Zuzana Lackova; Natalia Cernei; Milica Gagic; Vedran Milosavljevic; Vendula Smolikova; Eva Vaclavkova; Pavel Nevrkla; Pavel Knot; Olga Krystofova; David Hynek; Pavel Kopel; Jiri Skladanka; Vojtech Adam; Kristyna Smerkova. Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. Journal of Animal Science and Biotechnology 2019, 10, 1 -12.
AMA StylePavel Horky, Sylvie Skalickova, Lenka Urbankova, Daria Baholet, Silvia Kociova, Zuzana Bytešníková, Eliska Kabourkova, Zuzana Lackova, Natalia Cernei, Milica Gagic, Vedran Milosavljevic, Vendula Smolikova, Eva Vaclavkova, Pavel Nevrkla, Pavel Knot, Olga Krystofova, David Hynek, Pavel Kopel, Jiri Skladanka, Vojtech Adam, Kristyna Smerkova. Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure. Journal of Animal Science and Biotechnology. 2019; 10 (1):1-12.
Chicago/Turabian StylePavel Horky; Sylvie Skalickova; Lenka Urbankova; Daria Baholet; Silvia Kociova; Zuzana Bytešníková; Eliska Kabourkova; Zuzana Lackova; Natalia Cernei; Milica Gagic; Vedran Milosavljevic; Vendula Smolikova; Eva Vaclavkova; Pavel Nevrkla; Pavel Knot; Olga Krystofova; David Hynek; Pavel Kopel; Jiri Skladanka; Vojtech Adam; Kristyna Smerkova. 2019. "Zinc phosphate-based nanoparticles as a novel antibacterial agent: in vivo study on rats after dietary exposure." Journal of Animal Science and Biotechnology 10, no. 1: 1-12.
Magdalena Přibilová; Pavel Horký; Lenka Urbánková; Pavel Nevrkla; Jiří Skládanka. Influence of L-Carnitine Daily Supplement on Qualitative and Quantitative Ejaculate Indicators in Boars During the Summer Period. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 2018, 66, 1199 -1206.
AMA StyleMagdalena Přibilová, Pavel Horký, Lenka Urbánková, Pavel Nevrkla, Jiří Skládanka. Influence of L-Carnitine Daily Supplement on Qualitative and Quantitative Ejaculate Indicators in Boars During the Summer Period. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. 2018; 66 (5):1199-1206.
Chicago/Turabian StyleMagdalena Přibilová; Pavel Horký; Lenka Urbánková; Pavel Nevrkla; Jiří Skládanka. 2018. "Influence of L-Carnitine Daily Supplement on Qualitative and Quantitative Ejaculate Indicators in Boars During the Summer Period." Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis 66, no. 5: 1199-1206.
Pavel Horky; Sylvie Skalickova; Iva Caslavova; Amanda J. Deering; Pavel Nevrkla; Petr Slama; Vaclav Trojan; Jiri Skladanka. Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley. KVASNY PRUMYSL 2018, 64, 212 -216.
AMA StylePavel Horky, Sylvie Skalickova, Iva Caslavova, Amanda J. Deering, Pavel Nevrkla, Petr Slama, Vaclav Trojan, Jiri Skladanka. Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley. KVASNY PRUMYSL. 2018; 64 (5):212-216.
Chicago/Turabian StylePavel Horky; Sylvie Skalickova; Iva Caslavova; Amanda J. Deering; Pavel Nevrkla; Petr Slama; Vaclav Trojan; Jiri Skladanka. 2018. "Effect of fungicidal treatment and storage condition on content of selected mycotoxins in barley." KVASNY PRUMYSL 64, no. 5: 212-216.
Mycotoxins are toxic secondary metabolites produced by certain filamentous fungi. The occurrence of mycotoxins in food and feed causes negative health impacts on both humans and animals. Clay binders, yeast cell walls, or antioxidant additives are the most widely used products for mycotoxin elimination to reduce their impact. Although conventional methods are constantly improving, current research trends are looking for innovative solutions. Nanotechnology approaches seem to be a promising, effective, and low-cost way to minimize the health effects of mycotoxins. This review aims to shed light on the critical knowledge gap in mycotoxin elimination by nanotechnology. There are three main strategies: mold inhibition, mycotoxin adsorption, and reducing the toxic effect via nanoparticles. One of the most promising methods is the use of carbon-based nanomaterials. Graphene has been shown to have a huge surface and high binding capacity for mycotoxins. Attention has also been drawn to polymeric nanoparticles; they could substitute adsorbents or enclose any substance, which would improve the health status of the organism. In light of these findings, this review gives new insights into possible future research that might overcome challenges associated with nanotechnology utilization for mycotoxin elimination from agricultural products.
Pavel Horky; Sylvie Skalickova; Daria Baholet; Jiri Skladanka. Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins. Nanomaterials 2018, 8, 727 .
AMA StylePavel Horky, Sylvie Skalickova, Daria Baholet, Jiri Skladanka. Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins. Nanomaterials. 2018; 8 (9):727.
Chicago/Turabian StylePavel Horky; Sylvie Skalickova; Daria Baholet; Jiri Skladanka. 2018. "Nanoparticles as a Solution for Eliminating the Risk of Mycotoxins." Nanomaterials 8, no. 9: 727.
Hana Dočkalová; Pavel Horký; Ladislav Zeman; Jiří Skládanka. Influence of milk thistle pressed parts on rats liver histology. Potravinarstvo Slovak Journal of Food Sciences 2018, 12, 1 .
AMA StyleHana Dočkalová, Pavel Horký, Ladislav Zeman, Jiří Skládanka. Influence of milk thistle pressed parts on rats liver histology. Potravinarstvo Slovak Journal of Food Sciences. 2018; 12 (1):1.
Chicago/Turabian StyleHana Dočkalová; Pavel Horký; Ladislav Zeman; Jiří Skládanka. 2018. "Influence of milk thistle pressed parts on rats liver histology." Potravinarstvo Slovak Journal of Food Sciences 12, no. 1: 1.
Pavel Knot; Frantisek Hrabe; Stanislav Hejduk; Jiri Skladanka; Michal Kvasnovsky; Lucia Hodulikova; Iva Caslavova; Pavel Horky. The impacts of different management practices on botanical composition, quality, colour and growth of urban lawns. Urban Forestry & Urban Greening 2017, 26, 178 -183.
AMA StylePavel Knot, Frantisek Hrabe, Stanislav Hejduk, Jiri Skladanka, Michal Kvasnovsky, Lucia Hodulikova, Iva Caslavova, Pavel Horky. The impacts of different management practices on botanical composition, quality, colour and growth of urban lawns. Urban Forestry & Urban Greening. 2017; 26 ():178-183.
Chicago/Turabian StylePavel Knot; Frantisek Hrabe; Stanislav Hejduk; Jiri Skladanka; Michal Kvasnovsky; Lucia Hodulikova; Iva Caslavova; Pavel Horky. 2017. "The impacts of different management practices on botanical composition, quality, colour and growth of urban lawns." Urban Forestry & Urban Greening 26, no. : 178-183.