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Dr. Alexandros Chardas
Resident, Veterinary Pathology

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Journal article
Published: 14 May 2021 in Animals
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Canine gastric carcinoma (CGC) affects both sexes in relatively equal proportions, with a mean age of nine years, and the highest frequency in Staffordshire bull terriers. The most common histological subtype in 149 CGC cases was the undifferentiated carcinoma. CGCs were associated with increased chronic inflammation parameters and a greater chronic inflammatory score when Helicobacter spp. were present. Understanding the molecular pathways of gastric carcinoma is challenging. All markers showed variable expression for each subtype. Expression of the cell cycle regulator 14-3-3σ was positive in undifferentiated, tubular and papillary carcinomas. This demonstrates that 14-3-3σ could serve as an immunohistochemical marker in routine diagnosis and that mucinous, papillary and signet-ring cell (SRC) carcinomas follow a 14-3-3σ independent pathway. p16, another cell cycle regulator, showed increased expression in mucinous and SRC carcinomas. Expression of the adhesion molecules E-cadherin and CD44 appear context-dependent, with switching within tumor emboli potentially playing an important role in tumor cell survival, during invasion and metastasis. Within neoplastic emboli, acinar structures lacked expression of all markers, suggesting an independent molecular pathway that requires further investigation. These findings demonstrate similarities and differences between dogs and humans, albeit further clinicopathological data and molecular analysis are required.

ACS Style

Alexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals 2021, 11, 1409 .

AMA Style

Alexandros Hardas, Alejandro Suárez-Bonnet, Sam Beck, William Becker, Gustavo Ramírez, Simon Priestnall. Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart. Animals. 2021; 11 (5):1409.

Chicago/Turabian Style

Alexandros Hardas; Alejandro Suárez-Bonnet; Sam Beck; William Becker; Gustavo Ramírez; Simon Priestnall. 2021. "Canine Gastric Carcinomas: A Histopathological and Immunohistochemical Study and Similarities with the Human Counterpart." Animals 11, no. 5: 1409.

Journal article
Published: 24 July 2020 in Cancers
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Downregulation of the cylindromatosis (CYLD) tumor suppressor has been associated with breast cancer development and progression. Here, we report a critical role for CYLD in maintaining the phenotype of mammary epithelial cells in vitro and in vivo. CYLD downregulation or inactivation induced an epithelial to mesenchymal transition of mammary epithelial cells that was dependent on the concomitant activation of the transcription factors Yes-associated protein (YAP)/transcriptional coactivator with PDZ-binding motif (TAZ) and transforming growth factor beta (TGF)signaling. CYLD inactivation enhanced the nuclear localization of YAP/TAZ and the phosphorylation of Small Mothers Against Decapentaplegic (SMAD)2/3 proteins in confluent cell culture conditions. Consistent with these findings were the hyperplastic alterations of CYLD-deficient mouse mammary epithelia, which were associated with enhanced nuclear expression of the YAP/TAZ transcription factors. Furthermore, in human breast cancer samples, downregulation of CYLD expression correlates with enhanced YAP/TAZ-regulated target gene expression. Our results identify CYLD as a critical regulator of a signaling node that prevents the coordinated activation of YAP/TAZ and the TGF pathway in mammary epithelial cells, in order to maintain their phenotypic identity and homeostasis. Consequently, they provide a novel conceptual framework that supports and explains a causal implication of deficient CYLD expression in aggressive human breast cancers.

ACS Style

Athanasios Pseftogas; Konstantinos Xanthopoulos; Theofilos Poutahidis; Chrysanthi Ainali; Dimitra Dafou; Emmanuel Panteris; Joseph G. Kern; Xaralabos Varelas; Alexander Hardas; Christos Gonidas; Anastasia Tsingotjidou; Eudoxia Hatzivassiliou; George Mosialos. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers 2020, 12, 2047 .

AMA Style

Athanasios Pseftogas, Konstantinos Xanthopoulos, Theofilos Poutahidis, Chrysanthi Ainali, Dimitra Dafou, Emmanuel Panteris, Joseph G. Kern, Xaralabos Varelas, Alexander Hardas, Christos Gonidas, Anastasia Tsingotjidou, Eudoxia Hatzivassiliou, George Mosialos. The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling. Cancers. 2020; 12 (8):2047.

Chicago/Turabian Style

Athanasios Pseftogas; Konstantinos Xanthopoulos; Theofilos Poutahidis; Chrysanthi Ainali; Dimitra Dafou; Emmanuel Panteris; Joseph G. Kern; Xaralabos Varelas; Alexander Hardas; Christos Gonidas; Anastasia Tsingotjidou; Eudoxia Hatzivassiliou; George Mosialos. 2020. "The Tumor Suppressor CYLD Inhibits Mammary Epithelial to Mesenchymal Transition by the Coordinated Inhibition of YAP/TAZ and TGF Signaling." Cancers 12, no. 8: 2047.

Original research
Published: 01 June 2020 in OncoTargets and Therapy
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Background: Hepatocellular carcinoma (HCC) is a frequently diagnosed cancer and a leading cause of cancer-related death worldwide. Its rapid progression, combined with the limited treatment options at late stages, imposes the need for early detection and aggressive intervention. Based on the knowledge that hepatocarcinogenesis is significantly influenced by histone acetylation, we directed our search for novel HCC therapeutics among histone deacetylation inhibitors (HDACi). The aim of the present study was to investigate the effect of HDAC1/2 inhibitor Romidepsin in the well-established mouse model of diethylnitrosamine (DEN)-induced HCC. Materials and Methods: C56BL/6 mice were treated with Romidepsin at the critical point of 10 months after DEN challenge and their livers were examined 2 months later using histopathology and morphometry. Protein levels were assessed in serum using ELISA and in liver tissues using Western blot and immunohistochemistry (in-situ detection). Gene expression was quantified using real-time PCR. Results: Romidepsin suppressed cancer progression. This effect was associated with decreased proliferation and increased apoptosis of cancer cells. The cell cycle regulator CK2a, the anti-inflammatory molecule PPAR-γ, and the tumor suppressors PTEN and CYLD were upregulated in treated HCC. By contrast, the expression of PI3K, NF-κB p65 and c-Jun was reduced. In line with this result, the levels of two major apoptosis regulators, ie, BAD and the multifunctional protein c-Met, were lower in the blood serum of treated mice compared to the untreated mice with HCC. Conclusion: These findings suggest that Romidepsin, a drug currently used in the treatment of lymphoma, could also be considered in the management of early-stage HCC.

ACS Style

Hara Afaloniati; Katerina Angelopoulou; Alexander Giakoustidis; Alexandros Hardas; Athanasios Pseftogas; Kali Makedou; Athanasios Gargavanis; Thomas Goulopoulos; Stavros Iliadis; Vasileios Papadopoulos; Apostolos Papalois; George Mosialos; Theofilos Poutahidis; Dimitrios Giakoustidis. HDAC1/2 Inhibitor Romidepsin Suppresses DEN-Induced Hepatocellular Carcinogenesis in Mice. OncoTargets and Therapy 2020, ume 13, 5575 -5588.

AMA Style

Hara Afaloniati, Katerina Angelopoulou, Alexander Giakoustidis, Alexandros Hardas, Athanasios Pseftogas, Kali Makedou, Athanasios Gargavanis, Thomas Goulopoulos, Stavros Iliadis, Vasileios Papadopoulos, Apostolos Papalois, George Mosialos, Theofilos Poutahidis, Dimitrios Giakoustidis. HDAC1/2 Inhibitor Romidepsin Suppresses DEN-Induced Hepatocellular Carcinogenesis in Mice. OncoTargets and Therapy. 2020; ume 13 ():5575-5588.

Chicago/Turabian Style

Hara Afaloniati; Katerina Angelopoulou; Alexander Giakoustidis; Alexandros Hardas; Athanasios Pseftogas; Kali Makedou; Athanasios Gargavanis; Thomas Goulopoulos; Stavros Iliadis; Vasileios Papadopoulos; Apostolos Papalois; George Mosialos; Theofilos Poutahidis; Dimitrios Giakoustidis. 2020. "HDAC1/2 Inhibitor Romidepsin Suppresses DEN-Induced Hepatocellular Carcinogenesis in Mice." OncoTargets and Therapy ume 13, no. : 5575-5588.

Paper
Published: 22 April 2020 in Journal of Small Animal Practice
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To summarise the clinical presentation and outcomes in a series of miniature schnauzers diagnosed with histiocytic sarcoma. Retrospective review of medical records of miniature schnauzers diagnosed with histiocytic sarcoma between 2008 and 2019 at two referral centres in the UK. Signalment, clinical signs at initial presentation, imaging results and clinico- and histopathological findings, treatment type and outcome were recorded. Progression-free survival and overall survival time were calculated. Thirty dogs were included. Twenty-four of 29 dogs undergoing imaging of the thorax had lung and/or mediastinal involvement. The median overall survival time for dogs that were not euthanased within 3 days of diagnosis was 117 days (range 10 to 790). Three dogs underwent surgery; 13 received treatment with lomustine as a sole therapy - with partial responses documented on imaging in five of six dogs and 11 of 13 showing clinical improvement. Histiocytic sarcoma should be considered as a differential diagnosis for miniature schnauzers with pulmonary masses. Although responses to treatment were common, they were usually short-lived because of the aggressive nature of the disease.

ACS Style

K. Purzycka; L. M. Peters; J. Elliott; C. R. Lamb; S. L. Priestnall; Alexandros Hardas; C. A. Johnston; I. Rodriguez‐Piza. Histiocytic sarcoma in miniature schnauzers: 30 cases. Journal of Small Animal Practice 2020, 61, 338 -345.

AMA Style

K. Purzycka, L. M. Peters, J. Elliott, C. R. Lamb, S. L. Priestnall, Alexandros Hardas, C. A. Johnston, I. Rodriguez‐Piza. Histiocytic sarcoma in miniature schnauzers: 30 cases. Journal of Small Animal Practice. 2020; 61 (6):338-345.

Chicago/Turabian Style

K. Purzycka; L. M. Peters; J. Elliott; C. R. Lamb; S. L. Priestnall; Alexandros Hardas; C. A. Johnston; I. Rodriguez‐Piza. 2020. "Histiocytic sarcoma in miniature schnauzers: 30 cases." Journal of Small Animal Practice 61, no. 6: 338-345.

Journal article
Published: 17 September 2019 in Oncotarget
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// Bernard J. Varian 1 , Theofilos Poutahidis 1 , 2 , Gordon Haner 1 , Alex Hardas 2 , Vanessa Lau 1 and Susan E. Erdman 1 1 Division of Comparative Medicine, Massachusetts Institute of Technology, Cambridge, MA 02139, United States 2 Department of infectious Diseases and Pathology, Faculty of Veterinary Medicine, Aristotle University of Thessaloniki, 54124, Greece Correspondence to: Susan E. Erdman, email: [email protected] Keywords: body weight; mouse; exotoxin subunit B; CLS; inflammation Received: April 17, 2019 Accepted: July 05, 2019 Published: September 17, 2019 ABSTRACT During the past forty years there has been an inexplicable increase in chronic inflammatory disorders, including obesity. One theory, the hygiene hypothesis, involves dysregulated immunity arising after too few beneficial early life microbe exposures. Indeed, earlier studies have shown that gut microbe-immune interactions contribute to smoldering inflammation, adiposity, and weight gain. Here we tested a safe and well-established microbe-based immune adjuvant to restore immune homeostasis and counteract inflammation-associated obesity in animal models. We found that consuming Vibrio cholerae exotoxin subunit B (ctB) was sufficient to inhibit age-associated obesogenic outcomes in wild type mice, including reduced crown-like structures (CLS) and granulomatous necrosis histopathology in fat depots. Administration of cholera toxin reduced weight gain irrespective of age during administration; however, exposure during youth imparted greater slenderizing effects when compared with animals receiving ctB for the first time during adulthood. Beneficial effects were transplantable to other obesity-prone animals using immune cells alone, demonstrating an immune-mediated mechanism. Taken together, we concluded that oral vaccination with cholera toxin B helps stimulate health-protective immune responses that counteract age-associated obesity.

ACS Style

Bernard J. Varian; Theofilos Poutahidis; Gordon Haner; Alex Hardas; Vanessa Lau; Susan E. Erdman; Yoshito Terai; Masaaki Takai; Satoe Fujiwara; Yoshimichi Tanaka; Tomohito Tanaka; Hiroshi Sasaki; Naokazu Ibuki; Takanobu Ubai; Kazuhiro Yamamoto; Haruhito Azuma; Masahide Ohmichi. Consuming cholera toxin counteracts age-associated obesity. Oncotarget 2019, 10, 5497 -5509.

AMA Style

Bernard J. Varian, Theofilos Poutahidis, Gordon Haner, Alex Hardas, Vanessa Lau, Susan E. Erdman, Yoshito Terai, Masaaki Takai, Satoe Fujiwara, Yoshimichi Tanaka, Tomohito Tanaka, Hiroshi Sasaki, Naokazu Ibuki, Takanobu Ubai, Kazuhiro Yamamoto, Haruhito Azuma, Masahide Ohmichi. Consuming cholera toxin counteracts age-associated obesity. Oncotarget. 2019; 10 (53):5497-5509.

Chicago/Turabian Style

Bernard J. Varian; Theofilos Poutahidis; Gordon Haner; Alex Hardas; Vanessa Lau; Susan E. Erdman; Yoshito Terai; Masaaki Takai; Satoe Fujiwara; Yoshimichi Tanaka; Tomohito Tanaka; Hiroshi Sasaki; Naokazu Ibuki; Takanobu Ubai; Kazuhiro Yamamoto; Haruhito Azuma; Masahide Ohmichi. 2019. "Consuming cholera toxin counteracts age-associated obesity." Oncotarget 10, no. 53: 5497-5509.

Nutrition and metabolism
Published: 16 January 2018 in British Poultry Science
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1. The study aimed to investigate the effect of lysolecithin supplementation in low-energy diets on growth, nutrient digestibility and intestinal mucosa characteristics of broilers. 2. A total of 800 one-d-old Ross 308 broiler chickens were assigned to 4 dietary treatments consisting of 10 replicates of 20 broilers each. Broilers were fed with 4 different diets: (i) HE: positive control group broilers received a diet with unaltered energy; (ii) LE: negative control group broilers received a diet with lower energy of about 0.27 MJ/kg; (iii) LElys500: broilers received a diet similar to LE supplemented with 500 g/tn lysolecithin product (Lysoforte Booster DryTM); and (iv) LElys300: broilers received a diet similar to LE supplemented with 300 g/tn lysolecithin product. The experimental period was 42 d. 3. Body weight gain in treatments HE was higher than LE during the overall experimental period, while LElys500 and LElys300 had intermediate values. Feed conversion ratio was lower in HE and LElys500 than LE group, while the LElys300 had intermediate values. Fat digestibility was improved in both LElys 500 and LElys300 compared to the HE group. Apparent metabolisable energy (AMEn) was higher in HE, LElys500 and LElys300 than LE. Ileum viscosity at 42 d was also affected, being higher in LE group compared to HE. At 28 d mucosal thickness was lower both in LElys500 and LElys300 compared to HE and LE, while no difference occurred between treatment proliferation patterns of duodenal epithelial cells. 4. These findings indicated that lysolecithin supplementation at 500 g/tn of feed in low-energy diets maintained broiler performance. Supplementation of reformulated low-energy diets induced an increase in digesta viscosity. Lysolecithin supplementation resulted in variable alterations in the duodenum mucosal morphology.

ACS Style

G. A. Papadopoulos; T. Poutahidis; S. Chalvatzi; M. Di Benedetto; Alexandros Hardas; Vasileios Tsiouris; I. Georgopoulou; Georgios Arsenos; P. D. Fortomaris. Effects of lysolecithin supplementation in low-energy diets on growth performance, nutrient digestibility, viscosity and intestinal morphology of broilers. British Poultry Science 2018, 59, 232 -239.

AMA Style

G. A. Papadopoulos, T. Poutahidis, S. Chalvatzi, M. Di Benedetto, Alexandros Hardas, Vasileios Tsiouris, I. Georgopoulou, Georgios Arsenos, P. D. Fortomaris. Effects of lysolecithin supplementation in low-energy diets on growth performance, nutrient digestibility, viscosity and intestinal morphology of broilers. British Poultry Science. 2018; 59 (2):232-239.

Chicago/Turabian Style

G. A. Papadopoulos; T. Poutahidis; S. Chalvatzi; M. Di Benedetto; Alexandros Hardas; Vasileios Tsiouris; I. Georgopoulou; Georgios Arsenos; P. D. Fortomaris. 2018. "Effects of lysolecithin supplementation in low-energy diets on growth performance, nutrient digestibility, viscosity and intestinal morphology of broilers." British Poultry Science 59, no. 2: 232-239.

Journal article
Published: 01 January 2018 in Journal of Cancer
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: To elucidate the expression of Aurora kinases (AURK) and the anticancer effects of pan-aurora kinase inhibitor Danusertib in hepatocarcinogenesis model in C56Bl6 mice. : Thirty mice C56Bl6 were randomly divided into Group A or control, Group B animals who underwent experimental hepatocarcinogenesis with diethylnitrosamine (DEN), and Group C animals with DEN-induced hepatocarcinogenenesis that treated with pan-aurora kinase inhibitor Danusertib. Primary antibodies for immunochistochemistry (IHC) included rabbit antibodies against Ki-67, DKK1, INCENP, cleaved caspase-3, NF-κB p65, c-Jun, β-catenin. Hepatocyte growth factor receptor (C-MET/HGFR) and Bcl-2 antagonist of cell death (BAD) serum levels were determined using a quantitative sandwich enzyme immunoassay technique. : Inhibition of AURK reduced the number of DEN-induced liver tumours. Apoptosis and proliferation was very low in both DEN-induced and anti- AURK groups respectively. The hepatocellular adenoma cells of DEN-treated mice uniformly had ample nuclear INCENP whereas in anti- AURK markedly decreased. Expression of β-catenin, NF-kB and c-Jun did not differ in liver tumors of both AURK -depleted and non-depleted mice. Depletion of AURK reduced the number of DEN-induced hepatic tumours. However, their size did not differ significantly between the groups.

ACS Style

Paschalis Gavriilidis; Theofilos Poutahidis; Alexander Giakoustidis; Kali Makedou; Katerina Angelopoulou; Alexander Hardas; Paola Andreani; Argyro Zacharioudaki; George Saridis; Athanasios Gargavanis; Eleni Louri; Nikolaos Antoniadis; Eleftheria Karampela; Nikolaos Psychalakis; Antonios Michalopoulos; Apostolos Papalois; Stavros Iliadis; Satvinder Mudan; Daniel Azoulay; Dimitrios Giakoustidis. Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib. Journal of Cancer 2018, 9, 914 -922.

AMA Style

Paschalis Gavriilidis, Theofilos Poutahidis, Alexander Giakoustidis, Kali Makedou, Katerina Angelopoulou, Alexander Hardas, Paola Andreani, Argyro Zacharioudaki, George Saridis, Athanasios Gargavanis, Eleni Louri, Nikolaos Antoniadis, Eleftheria Karampela, Nikolaos Psychalakis, Antonios Michalopoulos, Apostolos Papalois, Stavros Iliadis, Satvinder Mudan, Daniel Azoulay, Dimitrios Giakoustidis. Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib. Journal of Cancer. 2018; 9 (5):914-922.

Chicago/Turabian Style

Paschalis Gavriilidis; Theofilos Poutahidis; Alexander Giakoustidis; Kali Makedou; Katerina Angelopoulou; Alexander Hardas; Paola Andreani; Argyro Zacharioudaki; George Saridis; Athanasios Gargavanis; Eleni Louri; Nikolaos Antoniadis; Eleftheria Karampela; Nikolaos Psychalakis; Antonios Michalopoulos; Apostolos Papalois; Stavros Iliadis; Satvinder Mudan; Daniel Azoulay; Dimitrios Giakoustidis. 2018. "Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib." Journal of Cancer 9, no. 5: 914-922.

Journal article
Published: 01 December 2017 in Experimental Cell Research
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Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of inflammation-driven intestinal neoplasmatogenesis, in which chronic colitis and CRC are induced using dextran sodium sulfate (DSS). In this report, we explored the contribution of intracellular components of Smad-mediated BMP signal transduction using the same model. Interestingly, upon DSS treatment, we noticed an overexpression of Runx1/2/3 transcription factors in both wild-type and uPA-deficient mice. Moreover, Runx1 and Runx2 expression levels exhibited an even higher increase in DSS-treated/uPA-deficient mice as compared to DSS-treated/wild-type animals. In all experimental conditions, in situ investigation of Runx-expressing cell types, revealed detection of all three Runx in the immune cells, yet in the DSS-treated/uPA-deficient mice Runx1 and Runx2 were also identified in the preneoplastic epithelium of advanced high-grade dysplasia and carcinoma in-situ colonic lesions. Finally, the uPA-deficient pro-tumorigenic colitic microenvironment exhibited increased levels of the Runx-induced target genes Snai2, Bim and Claudin1, known to have a role in tumor development and progression. These findings suggest that the absence of uPA correlates with increased levels of Runx transcriptional regulators in a way that promotes inflammation-associated carcinogenesis.

ACS Style

Hara Afaloniati; George S. Karagiannis; Alexandros Hardas; Theofilos Poutahidis; Katerina Angelopoulou. Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators. Experimental Cell Research 2017, 361, 257 -264.

AMA Style

Hara Afaloniati, George S. Karagiannis, Alexandros Hardas, Theofilos Poutahidis, Katerina Angelopoulou. Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators. Experimental Cell Research. 2017; 361 (2):257-264.

Chicago/Turabian Style

Hara Afaloniati; George S. Karagiannis; Alexandros Hardas; Theofilos Poutahidis; Katerina Angelopoulou. 2017. "Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators." Experimental Cell Research 361, no. 2: 257-264.

Controlled clinical trial
Published: 01 December 2017 in Research in Veterinary Science
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The aim of the study was to test two encapsulated regimens containing organic acids and/or zinc oxide (ZnO) on weaned piglet performance and jejunal mucosa morphology and immunity. For that, weaned piglets were allocated to treatments including control, supplemented with encapsulated organic acids (ACID group), and supplemented with organic acids and ZnO, both encapsulated (ACIDplus group). Antibiotics were used at similar concentrations in all groups during the first two weeks, but withdrawn from the ACIDplus group during the last three weeks of the experiment. ZnO was given with feed in the Control and ACID groups only during the first two weeks. The experimental period lasted 5 weeks. Piglets from the ACID group exhibited higher average daily gain compared to other groups during the last 3 weeks of the experiment (P<0.05). The ACIDplus group performed similarly with controls. The mucosal height of jejunum was higher in both ACID (P<0.01) and ACIDplus groups compared to controls (P<0.05). Immunohistochemical analysis of jejunal mucosa, showed higher numbers of neutrophils in ACID and ACIDplus groups compared to controls (P<0.01 and P<0.001, respectively). Treatments had the opposite effect on mucosal regulatory T-cells (Foxp3-positive cells) in jejunum, being higher (P<0.001) in control group compared to ACID and ACIDplus groups. The number of CD3-positive cells was higher (P<0.05) in the ACIDplus and control groups compared to the ACID group. In conclusion, the encapsulated products used had beneficial effects on growth performance coexisting with improvements on jejunal histomorphology and modulation of mucosal immunity.

ACS Style

Georgios Papadopoulos; Theofilos Poutahidis; Nicola Tallarico; Alexandros Hardas; Konstantinos Teliousis; Georgios Arsenos; Paschalis D. Fortomaris. Dietary supplementation of encapsulated organic acids enhances performance and modulates immune regulation and morphology of jejunal mucosa in piglets. Research in Veterinary Science 2017, 115, 174 -182.

AMA Style

Georgios Papadopoulos, Theofilos Poutahidis, Nicola Tallarico, Alexandros Hardas, Konstantinos Teliousis, Georgios Arsenos, Paschalis D. Fortomaris. Dietary supplementation of encapsulated organic acids enhances performance and modulates immune regulation and morphology of jejunal mucosa in piglets. Research in Veterinary Science. 2017; 115 ():174-182.

Chicago/Turabian Style

Georgios Papadopoulos; Theofilos Poutahidis; Nicola Tallarico; Alexandros Hardas; Konstantinos Teliousis; Georgios Arsenos; Paschalis D. Fortomaris. 2017. "Dietary supplementation of encapsulated organic acids enhances performance and modulates immune regulation and morphology of jejunal mucosa in piglets." Research in Veterinary Science 115, no. : 174-182.

Journal article
Published: 15 November 2017 in Oncology Letters
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Recent evidence has suggested that downregulation of the Wnt/β-catenin signaling pathway may contribute to the development and growth of HCC. Consequently, elements of this pathway have begun to emerge as potential targets for improving outcomes of anti-HCC. Thus, the present study sought to examine the effects of Wnt-1 blockade using the classical diethylnitrosamine (DEN)-induced chemical carcinogenesis mouse model of HCC. The depletion of Wnt-1 using neutralizing antisera was done for ten consecutive days at the age of 9 months and mice were examined for the following 20 days. At that time, DEN-treated mice had multiple variably-sized hepatic cell adenomas. Anti-Wnt-1 was particularly potent in suppressing the expression of critical elements of the Wnt/β-catenin signaling pathway, such as β-catenin and Frizzled-1 receptor, however, not Dickkopf-related protein 1. This effect co-existed with the suppression of Cyclin D1, FOXM1, NF-κΒ and c-Jun commensurate with proliferation and apoptosis blockade in hepatocellular adenomas, and reduced Bcl-2 and c-Met in the serum of mice. Nonetheless, tumor size and multiplicity were found to be unaffected, suggesting that apoptosis may be equally important to proliferation in the context of counteracting DEN induced hepatocellular adenomas of mice.

ACS Style

Argyrios Sklavos; Theofilos Poutahidis; Alexander Giakoustidis; Kali Makedou; Katerina Angelopoulou; Alexandros Hardas; Paola Andreani; Argyro Zacharioudaki; George Saridis; Thomas Goulopoulos; Kalliopi Tsarea; Maria Karamperi; Vassilios Papadopoulos; Vassilios Papanikolaou; Apostolos Papalois; Stavros Iliadis; Satvinder Mudan; Daniel Azoulay; Dimitrios Giakoustidis. Effects of Wnt‑1 blockade in DEN‑induced hepatocellular adenomas of mice. Oncology Letters 2017, 15, 1211 -1219.

AMA Style

Argyrios Sklavos, Theofilos Poutahidis, Alexander Giakoustidis, Kali Makedou, Katerina Angelopoulou, Alexandros Hardas, Paola Andreani, Argyro Zacharioudaki, George Saridis, Thomas Goulopoulos, Kalliopi Tsarea, Maria Karamperi, Vassilios Papadopoulos, Vassilios Papanikolaou, Apostolos Papalois, Stavros Iliadis, Satvinder Mudan, Daniel Azoulay, Dimitrios Giakoustidis. Effects of Wnt‑1 blockade in DEN‑induced hepatocellular adenomas of mice. Oncology Letters. 2017; 15 (1):1211-1219.

Chicago/Turabian Style

Argyrios Sklavos; Theofilos Poutahidis; Alexander Giakoustidis; Kali Makedou; Katerina Angelopoulou; Alexandros Hardas; Paola Andreani; Argyro Zacharioudaki; George Saridis; Thomas Goulopoulos; Kalliopi Tsarea; Maria Karamperi; Vassilios Papadopoulos; Vassilios Papanikolaou; Apostolos Papalois; Stavros Iliadis; Satvinder Mudan; Daniel Azoulay; Dimitrios Giakoustidis. 2017. "Effects of Wnt‑1 blockade in DEN‑induced hepatocellular adenomas of mice." Oncology Letters 15, no. 1: 1211-1219.

Randomized controlled trial
Published: 05 November 2016 in Brain, Behavior, and Immunity
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Neuropeptide hormone oxytocin has roles in social bonding, energy metabolism, and wound healing contributing to good physical, mental and social health. It was previously shown that feeding of a human commensal microbe Lactobacillus reuteri (L. reuteri) is sufficient to up-regulate endogenous oxytocin levels and improve wound healing capacity in mice. Here we show that oral L. reuteri-induced skin wound repair benefits extend to human subjects. Further, dietary supplementation with a sterile lysate of this microbe alone is sufficient to boost systemic oxytocin levels and improve wound repair capacity. Oxytocin-producing cells were found to be increased in the caudal paraventricular nucleus [PVN] of the hypothalamus after feeding of a sterile lysed preparation of L. reuteri, coincident with lowered blood levels of stress hormone corticosterone and more rapid epidermal closure, in mouse models. We conclude that microbe viability is not essential for regulating host oxytocin levels. The results suggest that a peptide or metabolite produced by bacteria may modulate host oxytocin secretion for potential public or personalized health goals.

ACS Style

Bernard J. Varian; Theofilos Poutahidis; Brett T. DiBenedictis; Tatiana Levkovich; Yassin Ibrahim; Eliska Didyk; Lana Shikhman; Harry K. Cheung; Alexandros Hardas; Catherine E. Ricciardi; Kumaran Kolandaivelu; Alexa H. Veenema; Eric J. Alm; Susan E. Erdman. Microbial lysate upregulates host oxytocin. Brain, Behavior, and Immunity 2016, 61, 36 -49.

AMA Style

Bernard J. Varian, Theofilos Poutahidis, Brett T. DiBenedictis, Tatiana Levkovich, Yassin Ibrahim, Eliska Didyk, Lana Shikhman, Harry K. Cheung, Alexandros Hardas, Catherine E. Ricciardi, Kumaran Kolandaivelu, Alexa H. Veenema, Eric J. Alm, Susan E. Erdman. Microbial lysate upregulates host oxytocin. Brain, Behavior, and Immunity. 2016; 61 ():36-49.

Chicago/Turabian Style

Bernard J. Varian; Theofilos Poutahidis; Brett T. DiBenedictis; Tatiana Levkovich; Yassin Ibrahim; Eliska Didyk; Lana Shikhman; Harry K. Cheung; Alexandros Hardas; Catherine E. Ricciardi; Kumaran Kolandaivelu; Alexa H. Veenema; Eric J. Alm; Susan E. Erdman. 2016. "Microbial lysate upregulates host oxytocin." Brain, Behavior, and Immunity 61, no. : 36-49.