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Dr. Gioele Capillo
University of > Messina, Department of Veterinary Sciences

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0 Aquaculture
0 Biodiversity
0 Fish immunology
0 environmental conservation
0 Fish Respiration

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Journal article
Published: 24 July 2021 in Zoology
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Heteropneustes fossilis is an air-breathing teleost inhabiting environments with very poor O2 conditions, and so it has evolved to cope with hypoxia. In the gills and respiratory air-sac, the sites for O2 sensing and the response to hypoxia rely on the expression of acetylcholine (Ach) acting via its nicotinic receptor (nAChR). This study examined the expression patterns of neuronal markers and some compounds in the NECs of the gills and respiratory air sac having an immunomodulatory function in mammalian lungs. Mucous cells, epithelial cells and neuroepithelial cells (NECs) were immunopositive to a variety of both neuronal markers (VAChT, nAChR, GABA-B-R1 receptor, GAD679) and the antimicrobial peptide piscidin, an evolutionary conserved humoral component of the mucosal immune system in fish. We speculate that Ach release via nAChR from mucous cells may be modulated by GABA production in the NECs and it is required for the induction of mucus production in both normoxic and hypoxic conditions. The presence of piscidin in mucous cells may act in synergy with the autocrine/paracrine signals of Ach and GABA binding to GABA B R1B receptor that may play a local immunomodulatory function in the mucous epithelia of the gills and the respiratory air sac. The potential role of the NECs in the immunobiological behaviour of the gill/air-sac is at moment a matter of speculation. The extent to which the NECs as such may participate is elusive at this stage and waits investigation.

ACS Style

Eugenia Rita Lauriano; Gioele Capillo; Jose Manuel Icardo; Jorge Manuel Oliveira Fernandes; Viswanath Kiron; Michal Kuciel; Krystyna Zuwala; Maria Cristina Guerrera; Marialuisa Aragona; Antonino Germana’; Giacomo Zaccone. Neuroepithelial cells (NECs) and mucous cells express a variety of neurotransmitters and neurotransmitter receptors in the gill and respiratory air-sac of the catfish Heteropneustes fossilis (Siluriformes, Heteropneustidae): a possible role in local immune defence. Zoology 2021, 148, 125958 .

AMA Style

Eugenia Rita Lauriano, Gioele Capillo, Jose Manuel Icardo, Jorge Manuel Oliveira Fernandes, Viswanath Kiron, Michal Kuciel, Krystyna Zuwala, Maria Cristina Guerrera, Marialuisa Aragona, Antonino Germana’, Giacomo Zaccone. Neuroepithelial cells (NECs) and mucous cells express a variety of neurotransmitters and neurotransmitter receptors in the gill and respiratory air-sac of the catfish Heteropneustes fossilis (Siluriformes, Heteropneustidae): a possible role in local immune defence. Zoology. 2021; 148 ():125958.

Chicago/Turabian Style

Eugenia Rita Lauriano; Gioele Capillo; Jose Manuel Icardo; Jorge Manuel Oliveira Fernandes; Viswanath Kiron; Michal Kuciel; Krystyna Zuwala; Maria Cristina Guerrera; Marialuisa Aragona; Antonino Germana’; Giacomo Zaccone. 2021. "Neuroepithelial cells (NECs) and mucous cells express a variety of neurotransmitters and neurotransmitter receptors in the gill and respiratory air-sac of the catfish Heteropneustes fossilis (Siluriformes, Heteropneustidae): a possible role in local immune defence." Zoology 148, no. : 125958.

Journal article
Published: 14 July 2021 in International Journal of Molecular Sciences
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Hyperpolarization-activated cyclic nucleotide-gated (HCN) channels are proteins that contain highly conserved functional domains and sequence motifs that are correlated with their unique biophysical activities, to regulate cardiac pacemaker activity and synaptic transmission. These pacemaker proteins have been studied in mammalian species, but little is known now about their heart distribution in lower vertebrates and c-AMP modulation. Here, we characterized the pacemaker system in the heart of the wild Atlantic cod (Gadus morhua), with respect to primary pacemaker molecular markers. Special focus is given to the structural, ultrastructural and molecular characterization of the pacemaker domain, through the expression of HCN channel genes and the immunohistochemistry of HCN isoforms, including the location of intracardiac neurons that are adjacent to the sinoatrial region of the heart. Similarly to zebrafish and mammals, these neurons are immunoreactive to ChAT, VAChT and nNOS. It has been shown that cardiac pacemaking can be modulated by sympathetic and parasympathetic pathways, and the existence of intracardiac neurons projecting back to the central nervous system provide a plausible link between them.

ACS Style

Gioele Capillo; Eugenia Lauriano; Jose Icardo; Prabhugouda Siriyappagouder; Michal Kuciel; Stelios Karapanagiotis; Giacomo Zaccone; Jorge Fernandes. Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758). International Journal of Molecular Sciences 2021, 22, 7539 .

AMA Style

Gioele Capillo, Eugenia Lauriano, Jose Icardo, Prabhugouda Siriyappagouder, Michal Kuciel, Stelios Karapanagiotis, Giacomo Zaccone, Jorge Fernandes. Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758). International Journal of Molecular Sciences. 2021; 22 (14):7539.

Chicago/Turabian Style

Gioele Capillo; Eugenia Lauriano; Jose Icardo; Prabhugouda Siriyappagouder; Michal Kuciel; Stelios Karapanagiotis; Giacomo Zaccone; Jorge Fernandes. 2021. "Structural Identification of the Pacemaker Cells and Expression of Hyperpolarization-Activated Cyclic Nucleotide-Gated (HCN) Channels in the Heart of the Wild Atlantic Cod, Gadus morhua (Linnaeus, 1758)." International Journal of Molecular Sciences 22, no. 14: 7539.

Journal article
Published: 08 April 2021 in Applied Sciences
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In the present study, it has been evaluated how 10 µm of polyethylene microspheres can be ingested by Artemia salina (Linnaeus, 1758) larvae within the first 7 days of the life cycle, and the impact on their health. Twelve A. salina larvae (instar I) groups were exposed to different microplastics (MPs) concentrations (0-1-10-102-103-104 MPs/mL), with and without Dunaliella salina as a food source. The results highlighted that A. salina larvae ingest MPs in relation to the exposure times in a dose-dependent manner and are significantly influenced by food availability. The highest contamination found was 306.2 MPs/individual at 104 MPs/mL exposure without a food source. No MPs were found in the presence of the food source from 1 to 102 MPs/mL, while contamination was detected at all concentrations of MPs without a food source. The worst effect on the developmental stages was evaluated at 168 h with a food source, with a delay compared to the control of I and II instars at 103 and 104 MPs/mL, respectively. Furthermore, microalgal feeding was significantly reduced for about 50% in the presence of 104 MPs/mL. These results highlight that aquatic microplastics pollution could affect the A. salina’s feeding behavior and life cycle.

ACS Style

Marco Albano; Giuseppe Panarello; Davide Di Paola; Fabiano Capparucci; Rosalia Crupi; Enrico Gugliandolo; Nunziacarla Spanò; Gioele Capillo; Serena Savoca. The Influence of Polystyrene Microspheres Abundance on Development and Feeding Behavior of Artemia salina (Linnaeus, 1758). Applied Sciences 2021, 11, 3352 .

AMA Style

Marco Albano, Giuseppe Panarello, Davide Di Paola, Fabiano Capparucci, Rosalia Crupi, Enrico Gugliandolo, Nunziacarla Spanò, Gioele Capillo, Serena Savoca. The Influence of Polystyrene Microspheres Abundance on Development and Feeding Behavior of Artemia salina (Linnaeus, 1758). Applied Sciences. 2021; 11 (8):3352.

Chicago/Turabian Style

Marco Albano; Giuseppe Panarello; Davide Di Paola; Fabiano Capparucci; Rosalia Crupi; Enrico Gugliandolo; Nunziacarla Spanò; Gioele Capillo; Serena Savoca. 2021. "The Influence of Polystyrene Microspheres Abundance on Development and Feeding Behavior of Artemia salina (Linnaeus, 1758)." Applied Sciences 11, no. 8: 3352.

Review
Published: 02 April 2021 in Sustainability
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We reviewed literature on the diet of the Galeus melastomus Rafinesque, 1810, from the Mediterranean Sea. Specific keywords (“Galeus melastomus diet”, “feeding habits”, “trophic position”, “biology”, “deep environment adaptation”) in the principal data sources, such as Web of Science, PubMed, and Google Scholar were used. Seventeen studies conducted on the diet and trophic position of G. melastomus have been considered for Mediterranean Sea regions. The feeding habits have been analyzed in many areas of the western basin; instead, for the Tyrrhenian, Adriatic, and central Mediterranean Seas, information is outdated and fragmentary. In all investigated sub areas, the data showed that G. melastomus is an opportunistic demersal supra benthic predator, benthic feeder, and scavenger, that adapts its diet to the seasonal and geographical fluctuations of the prey availability. It occupies a generalist niche showing individual specialization. In all reviewed Mediterranean sub areas, the most important prey groups were crustaceans, cephalopods, and teleost fishes. Taxa percentage in its diet composition can vary depending on different habitats with ontogenetic development of individuals, depth (that is correlated with the ontogenetic development), seasonal availability, and distribution of different prey groups. Widening knowledge of G. melastomus feeding habits is a fundamental tool for better understand meso and bathy-pelagic ecosystems.

ACS Style

Claudio D’Iglio; Serena Savoca; Paola Rinelli; Nunziacarla Spanò; Gioele Capillo. Diet of the Deep-Sea Shark Galeus melastomus Rafinesque, 1810, in the Mediterranean Sea: What We Know and What We Should Know. Sustainability 2021, 13, 3962 .

AMA Style

Claudio D’Iglio, Serena Savoca, Paola Rinelli, Nunziacarla Spanò, Gioele Capillo. Diet of the Deep-Sea Shark Galeus melastomus Rafinesque, 1810, in the Mediterranean Sea: What We Know and What We Should Know. Sustainability. 2021; 13 (7):3962.

Chicago/Turabian Style

Claudio D’Iglio; Serena Savoca; Paola Rinelli; Nunziacarla Spanò; Gioele Capillo. 2021. "Diet of the Deep-Sea Shark Galeus melastomus Rafinesque, 1810, in the Mediterranean Sea: What We Know and What We Should Know." Sustainability 13, no. 7: 3962.

Journal article
Published: 13 February 2021 in Fish & Shellfish Immunology
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Acetylcholine (Ach) is the main neurotransmitter in the neuronal cholinergic system and also works as a signaling molecule in non-neuronal cells and tissues. The diversity of signaling pathways mediated by Ach provides a basis for understanding the biology of the cholinergic epithelial cells and immune cells in the gill of the species studied. NECs in the gill were not found surprisingly, but specialized cells showing the morphological, histochemical and ultrastructural characteristics of eosinophils were located in the gill filaments and respiratory lamellae. Much remains unknown about the interaction between the nerves and eosinophils that modulate both the release of acetylcholine and its nicotinic and muscarinic receptors including the role of acetylcholine in the mechanisms of O2 chemosensing. In this study we report for the first time the expression of Ach in the pavement cells of the gill lamellae in fish, the mast cells associated with eosinophils and nerve interaction for both immune cell types, in the gill of the extant butterfly fish Pantodon buchholzi. Multiple roles have been hypothesized for Ach and alpha nAChR in the gills. Among these there are the possible involvement of the pavement cells of the gill lamellae as O2 chemosensitive cells, the interaction of Ach positive mast cells with eosinophils and interaction of eosinophils with nerve terminals. This could be related to the use of the vesicular acetylcholine transporter (VAChT) and the alpha 2 subunit of the acetylcholine nicotinic receptor (alpha 2 nAChR). These data demonstrate the presence of Ach multiple sites of neuronal and non-neuronal release and reception within the gill and its ancestral signaling that arose during the evolutionary history of this conservative fish species.

ACS Style

Gioele Capillo; Giacomo Zaccone; Camila Cupello; Jorge Manuel Oliveira Fernandes; Kiron Viswanath; Michal Kuciel; Krystyna Zuwala; Maria Cristina Guerrera; Marialuisa Aragona; Jose Manuel Icardo; Eugenia Rita Lauriano. Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae). Fish & Shellfish Immunology 2021, 111, 189 -200.

AMA Style

Gioele Capillo, Giacomo Zaccone, Camila Cupello, Jorge Manuel Oliveira Fernandes, Kiron Viswanath, Michal Kuciel, Krystyna Zuwala, Maria Cristina Guerrera, Marialuisa Aragona, Jose Manuel Icardo, Eugenia Rita Lauriano. Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae). Fish & Shellfish Immunology. 2021; 111 ():189-200.

Chicago/Turabian Style

Gioele Capillo; Giacomo Zaccone; Camila Cupello; Jorge Manuel Oliveira Fernandes; Kiron Viswanath; Michal Kuciel; Krystyna Zuwala; Maria Cristina Guerrera; Marialuisa Aragona; Jose Manuel Icardo; Eugenia Rita Lauriano. 2021. "Expression of acetylcholine, its contribution to regulation of immune function and O2 sensing and phylogenetic interpretations of the African butterfly fish Pantodon buchholzi (Osteoglossiformes, Pantodontidae)." Fish & Shellfish Immunology 111, no. : 189-200.

Journal article
Published: 28 December 2020 in Coatings
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New surface active ionic liquids (SAILs), based on cholinium cations and dodecylbenzenesulfonate as anion, have been synthesized and their potential application as antimicrobial colonization agents on cultural heritage (CH)stone materials investigated. The biocidal activity and antifouling capabilities were, preliminarily, evaluated by a screening on pure Gram (+) and Gram (−) bacteria strain cultures, yeasts, hyphomycetes and single-celled algae. Tests on stone materials (marble and tufa) vs a stabilized community, constituted by a mixture of microbial strains, revealed that some SAILs display both antimicrobial and preventive antibiofilm action against new colonization. Analogous tests have been performed on [email protected] precursors.

ACS Style

Filomena De Leo; Alessia Marchetta; Gioele Capillo; Antonino Germanà; Patrizia Primerano; Sandra Lo Schiavo; Clara Urzì. Surface Active Ionic Liquids Based Coatings as Subaerial Anti-Biofilms for Stone Built Cultural Heritage. Coatings 2020, 11, 26 .

AMA Style

Filomena De Leo, Alessia Marchetta, Gioele Capillo, Antonino Germanà, Patrizia Primerano, Sandra Lo Schiavo, Clara Urzì. Surface Active Ionic Liquids Based Coatings as Subaerial Anti-Biofilms for Stone Built Cultural Heritage. Coatings. 2020; 11 (1):26.

Chicago/Turabian Style

Filomena De Leo; Alessia Marchetta; Gioele Capillo; Antonino Germanà; Patrizia Primerano; Sandra Lo Schiavo; Clara Urzì. 2020. "Surface Active Ionic Liquids Based Coatings as Subaerial Anti-Biofilms for Stone Built Cultural Heritage." Coatings 11, no. 1: 26.

Journal article
Published: 24 September 2020 in Animals
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The environmental monitoring of chemical toxicants has been a widely studied topic in the last few decades. The main aim of the present study was to determine the total concentration of nine elements (Cd, Cr, Pb, Ni, Al, Cu, Fe, Mn, and Zn) in the fish species grey mullet (M. cephalus) and in the coastal marine waters collected from various sampling points along the Black Sea (Bulgaria) and the Ionian Sea (Italy). Further, those results were applied to predict the pollution degree in those coastal marine environments. The fish samples were subject to acid digestion followed by appropriate analytical determination. The metal concentrations in marine water samples collected from the Black Sea (Bulgaria) and the Ionian Sea (Italy) were also analyzed. Unpaired Student’s t-test and the one-way ANOVA were applied for the statistical analysis of the data. The statistical results revealed a significant variation (p < 0.0001) in the concentration of various fish tissues. The accumulation of toxic and essential elements differs significantly in grey mullet species caught from the Black Sea (Bulgaria) and the Ionian Sea (Italy). The results from this study may serve as a convenient approach during marine pollution programs set by both countries (Italy and Bulgaria).

ACS Style

Francesco Fazio; Claudio D’Iglio; Gioele Capillo; Concetta Saoca; Katya Peycheva; Giuseppe Piccione; Lubomir Makedonski. Environmental Investigations and Tissue Bioaccumulation of Heavy Metals in Grey Mullet from the Black Sea (Bulgaria) and the Ionian Sea (Italy). Animals 2020, 10, 1739 .

AMA Style

Francesco Fazio, Claudio D’Iglio, Gioele Capillo, Concetta Saoca, Katya Peycheva, Giuseppe Piccione, Lubomir Makedonski. Environmental Investigations and Tissue Bioaccumulation of Heavy Metals in Grey Mullet from the Black Sea (Bulgaria) and the Ionian Sea (Italy). Animals. 2020; 10 (10):1739.

Chicago/Turabian Style

Francesco Fazio; Claudio D’Iglio; Gioele Capillo; Concetta Saoca; Katya Peycheva; Giuseppe Piccione; Lubomir Makedonski. 2020. "Environmental Investigations and Tissue Bioaccumulation of Heavy Metals in Grey Mullet from the Black Sea (Bulgaria) and the Ionian Sea (Italy)." Animals 10, no. 10: 1739.

Review
Published: 09 September 2020 in Acta Histochemica
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Langerhans cells (LCs) are specialized dendritic cells (DCs) that play a defense role in recognizing foreign antigens, in tissue where antigenic exposures occur, as in the skin and mucous membranes. LCs are able to continuously move within the tissues thanks to dendritic contraction and distension performing their surveillance and/or phagocytosis role. These cells are characterized by the presence of Birbeck granules in their cytoplasm, involved in endocytosis. LCs have been characterized in several classes of vertebrates, from fish to mammals using different histological and molecular techniques. The aim of the present review is to define the state of art and the need of information about immunohistochemical markers of LCs in different classes of vertebrates. The most used immunohistochemical (IHC) markers are Langerin/CD207, CD1a, S-100 and TLR. These IHC markers are described in relation to their finding in different vertebrate classes with phylogenetical considerations. Among the four markers, Langerin/CD207 and TLR have the widest spectrum of cross reactivity in LCs.

ACS Style

Alessio Alesci; Eugenia Rita Lauriano; Marialuisa Aragona; Gioele Capillo; Simona Pergolizzi. Marking vertebrates langerhans cells, from fish to mammals. Acta Histochemica 2020, 122, 151622 -151622.

AMA Style

Alessio Alesci, Eugenia Rita Lauriano, Marialuisa Aragona, Gioele Capillo, Simona Pergolizzi. Marking vertebrates langerhans cells, from fish to mammals. Acta Histochemica. 2020; 122 (7):151622-151622.

Chicago/Turabian Style

Alessio Alesci; Eugenia Rita Lauriano; Marialuisa Aragona; Gioele Capillo; Simona Pergolizzi. 2020. "Marking vertebrates langerhans cells, from fish to mammals." Acta Histochemica 122, no. 7: 151622-151622.

Journal article
Published: 17 May 2020 in Tissue and Cell
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The constant exposure of ocular surface to external environment and then to several microbial agents is often related to the pathogenesis of various inflammatory eye disorders. In the present study α-Smooth Muscle Actin (α-SMA) and Langerin CD/207 expression and function was investigated in a rabbit corneal keratitis. The inflammation was induced by the secreted form of glycoprotein B (gB1s) of HSV-1, in an ex vivo rabbit corneal model. α-SMA is often used as a marker for myofibroblasts. In this study, for the first time, we show α-SMA positive corneal epithelial cells, during HSV-1 cornea inflammation, demonstrating a crucial role in wound healing, especially during remodeling phase. Furthermore, we show the presence of Dendritic Cells Langerin CD/207 positive, located mainly in the basal epithelial layer and in corneal stroma during the inflammatory processes. Our result validating the ex vivo organotypic rabbit corneal model, for the study about pathogenesis of HSV-1 ocular infection.

ACS Style

Simona Pergolizzi; Andreana Marino; Gioele Capillo; Marialuisa Aragona; Peggy Marconi; Eugenia Rita Lauriano. Expression of Langerin/CD 207 and α-smooth muscle actin in ex vivo rabbit corneal keratitis model. Tissue and Cell 2020, 66, 101384 .

AMA Style

Simona Pergolizzi, Andreana Marino, Gioele Capillo, Marialuisa Aragona, Peggy Marconi, Eugenia Rita Lauriano. Expression of Langerin/CD 207 and α-smooth muscle actin in ex vivo rabbit corneal keratitis model. Tissue and Cell. 2020; 66 ():101384.

Chicago/Turabian Style

Simona Pergolizzi; Andreana Marino; Gioele Capillo; Marialuisa Aragona; Peggy Marconi; Eugenia Rita Lauriano. 2020. "Expression of Langerin/CD 207 and α-smooth muscle actin in ex vivo rabbit corneal keratitis model." Tissue and Cell 66, no. : 101384.

Journal article
Published: 20 February 2020 in Science of The Total Environment
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We report the presence of microplastics on the external surface and in the gastrointestinal tract of white late-larval and juvenile stages (fry) of clupeid fishes caught in the Southern Tyrrhenian Sea. The average highest number of plastics debris was recorded on Sardina pilchardus (0.53 items/specimen); a lower average number of items was observed for Engraulis encrasicolus (0.26 items/specimen). The plastics were characterized by fibers that differed in shape, colour and composition. Polyester, polypropylene, polyacrylonitrile, polyethylene, polyamide, nylon, rayon and polyurethane segments were detected by Raman and FTIR spectroscopies. Traces of organic components and dyes, compounds that are generally included in the polymer matrix to modify its base properties, were also identified on microplastics. Our results raise concerns for the potential transfer of synthetic materials through the marine food web and into humans, given the prominent role of S. pilchardus and E. encrasicolus within the food web as main food source for many marine species.

ACS Style

Serena Savoca; Teresa Bottari; Enza Fazio; Martina Bonsignore; Monique Mancuso; Gian Marco Luna; Teresa Romeo; Luisa D'Urso; Gioele Capillo; Giuseppe Panarello; Silvestro Greco; Giuseppe Compagnini; Giovanni Lanteri; Rosalia Crupi; Fortunato Neri; Nunziacarla Spanò. Plastics occurrence in juveniles of Engraulis encrasicolus and Sardina pilchardus in the Southern Tyrrhenian Sea. Science of The Total Environment 2020, 718, 137457 .

AMA Style

Serena Savoca, Teresa Bottari, Enza Fazio, Martina Bonsignore, Monique Mancuso, Gian Marco Luna, Teresa Romeo, Luisa D'Urso, Gioele Capillo, Giuseppe Panarello, Silvestro Greco, Giuseppe Compagnini, Giovanni Lanteri, Rosalia Crupi, Fortunato Neri, Nunziacarla Spanò. Plastics occurrence in juveniles of Engraulis encrasicolus and Sardina pilchardus in the Southern Tyrrhenian Sea. Science of The Total Environment. 2020; 718 ():137457.

Chicago/Turabian Style

Serena Savoca; Teresa Bottari; Enza Fazio; Martina Bonsignore; Monique Mancuso; Gian Marco Luna; Teresa Romeo; Luisa D'Urso; Gioele Capillo; Giuseppe Panarello; Silvestro Greco; Giuseppe Compagnini; Giovanni Lanteri; Rosalia Crupi; Fortunato Neri; Nunziacarla Spanò. 2020. "Plastics occurrence in juveniles of Engraulis encrasicolus and Sardina pilchardus in the Southern Tyrrhenian Sea." Science of The Total Environment 718, no. : 137457.

Journal article
Published: 07 February 2020 in Zoology
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The air-breathing specialization has evolved idependently in vertebrates, as many different organs can perfom gas exchange. The largest obligate air-breathing fish from South America Arapaima gigas breathe air using its gas bladder, and its dependence on air breathing increases during its growth. During its development, gill morphology shows a dramatic change, remodeling with a gradual reduction of gill lamellae during the transition from water breathing to air breathing . It has been suggested that in this species the gills remain the main site of O2 and CO2 sensing. Consistent with this, we demonstrate for the first time the occurrence of the neuroepithelial cells (NECs) in the glottis, and in the gill filament epithelia and their distal halves. These cells contain a broader spectrum of neurotransmitters (5-HT, acetylcholine, nNOS), G-protein subunits and the muscarininic receptors that are coupled to G proteins (G-protein coupled receptors). We report also for the first time the presence of G alpha proteins coupled with muscarinic receptors on the NECs, that are thought as receptors that initiate the cardiorespiratory reflexes in aquatic vertebrates. Based on the specific orientation in the epithelia and their closest vicinity to efferent vasculatures, the gill and glottal NECs of A. gigas could be regarded as potential O2 and CO2 sensing receptors. However, future studies are needed to ascertain the neurophysiological characterization of these cells.

ACS Style

Giacomo Zaccone; Camila Cupello; Gioele Capillo; Michal Kuciel; Ana L.R. Nascimento; Anita Gopesh; Germana Patrizia Germanà; Nunziacarla Spanò; Maria Cristina Guerrera; Marialuisa Aragona; Rosalia Crupi; Jose Manuel Icardo; Eugenia Rita Lauriano. Expression of Acetylcholine- and G protein coupled Muscarinic receptor in the Neuroepithelial cells (NECs) of the obligated air-breathing fish, Arapaima gigas (Arapaimatidae: Teleostei). Zoology 2020, 139, 125755 .

AMA Style

Giacomo Zaccone, Camila Cupello, Gioele Capillo, Michal Kuciel, Ana L.R. Nascimento, Anita Gopesh, Germana Patrizia Germanà, Nunziacarla Spanò, Maria Cristina Guerrera, Marialuisa Aragona, Rosalia Crupi, Jose Manuel Icardo, Eugenia Rita Lauriano. Expression of Acetylcholine- and G protein coupled Muscarinic receptor in the Neuroepithelial cells (NECs) of the obligated air-breathing fish, Arapaima gigas (Arapaimatidae: Teleostei). Zoology. 2020; 139 ():125755.

Chicago/Turabian Style

Giacomo Zaccone; Camila Cupello; Gioele Capillo; Michal Kuciel; Ana L.R. Nascimento; Anita Gopesh; Germana Patrizia Germanà; Nunziacarla Spanò; Maria Cristina Guerrera; Marialuisa Aragona; Rosalia Crupi; Jose Manuel Icardo; Eugenia Rita Lauriano. 2020. "Expression of Acetylcholine- and G protein coupled Muscarinic receptor in the Neuroepithelial cells (NECs) of the obligated air-breathing fish, Arapaima gigas (Arapaimatidae: Teleostei)." Zoology 139, no. : 125755.

Journal article
Published: 27 November 2019 in Acta Histochemica
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The airways and lungs of vertebrates are an entrance way for several microbial pathogens. Cetaceans present an upper and lower respiratory anatomy that allows the rapid flow of large air volumes, which may lead to high susceptibility to respiratory infections. Mortality and stranding rate of Cetaceans increased dramatically, so wide the knowledge about the immune system and specific antibodies identifying immune cells populations, is of fundamental importance to monitor and document cetacean health. The aim of this study was to identify the localization of dendritic cells marked by Langerin/CD207 in airways, lungs and associated lymph nodes, of the striped dolphin Stenella coeruleoalba. Samples of trachea, bronchi, lungs and lung-associated lymph nodes were obtained from a stranded adult male of Stenella coeruleoalba. Our results showed abundant lymphoid aggregates (LAs) in the lung of S. ceruleoalba. Langerhans-like dendritic cells were well distributed along the epithelium and interstitium of respiratory tract and in associated lymph nodes. The present study deepens the knowledge about the cetacean’s immune system and report about the exploitability of a commercial antibody (Langerin/CD207) for cetacean species.

ACS Style

E.R. Lauriano; Simona Pergolizzi; P. Lo Cascio; M. Kuciel; N. Zizzo; M.C. Guerrera; M. Aragona; G Capillo. Expression of Langerin/CD207 in airways, lung and associated lymph nodes of a stranded striped dolphin (Stenella coeruleoalba). Acta Histochemica 2019, 122, 151471 .

AMA Style

E.R. Lauriano, Simona Pergolizzi, P. Lo Cascio, M. Kuciel, N. Zizzo, M.C. Guerrera, M. Aragona, G Capillo. Expression of Langerin/CD207 in airways, lung and associated lymph nodes of a stranded striped dolphin (Stenella coeruleoalba). Acta Histochemica. 2019; 122 (2):151471.

Chicago/Turabian Style

E.R. Lauriano; Simona Pergolizzi; P. Lo Cascio; M. Kuciel; N. Zizzo; M.C. Guerrera; M. Aragona; G Capillo. 2019. "Expression of Langerin/CD207 in airways, lung and associated lymph nodes of a stranded striped dolphin (Stenella coeruleoalba)." Acta Histochemica 122, no. 2: 151471.

Journal article
Published: 01 November 2019 in Science of The Total Environment
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Pollution deriving from textile wastes, including industrial and household waste, is recently of great interest due to their environmental impacts. Anthropogenic and synthetic fibers are responsible for negative effects on the quality of water and soil, and, also, their presence damages plant and animal health. In this work, the authors revealed the occurrence of man-made cellulose fibers in specimens of Boops boops from the Northern Sicilian coasts. Bogue was chosen as target species as it has been used as an indicator within the European Marine Strategy Framework Directive (MSFD 2008/56/EC) in order to value the "microplastics status" in the stomach contents. Of the 30 specimens examined, 63.3% of these had ingested fibers items. The number of fibers ranged from 1 to 10 per specimens with an average of 2.7 items/specimen. Fibers length ranged from 0.5 to 30 mm, most of them were black (95%), and a small percentage was red (5%). The ingestion of man-made cellulose fibers, observed for the first time, in Boops boops in the Mediterranean Sea wake-up call and it should attract the attention of the EU for new guidelines where this new type of contaminant is classified harmful as well as plastics.

ACS Style

Serena Savoca; Gioele Capillo; Monique Mancuso; Caterina Faggio; Giuseppe Panarello; Rosalia Crupi; Martina Bonsignore; Luisa D'Urso; Giuseppe Compagnini; Fortunato Neri; Enza Fazio; Teresa Romeo; Teresa Bottari; Nunziacarla Spanò. Detection of artificial cellulose microfibers in Boops boops from the northern coasts of Sicily (Central Mediterranean). Science of The Total Environment 2019, 691, 455 -465.

AMA Style

Serena Savoca, Gioele Capillo, Monique Mancuso, Caterina Faggio, Giuseppe Panarello, Rosalia Crupi, Martina Bonsignore, Luisa D'Urso, Giuseppe Compagnini, Fortunato Neri, Enza Fazio, Teresa Romeo, Teresa Bottari, Nunziacarla Spanò. Detection of artificial cellulose microfibers in Boops boops from the northern coasts of Sicily (Central Mediterranean). Science of The Total Environment. 2019; 691 ():455-465.

Chicago/Turabian Style

Serena Savoca; Gioele Capillo; Monique Mancuso; Caterina Faggio; Giuseppe Panarello; Rosalia Crupi; Martina Bonsignore; Luisa D'Urso; Giuseppe Compagnini; Fortunato Neri; Enza Fazio; Teresa Romeo; Teresa Bottari; Nunziacarla Spanò. 2019. "Detection of artificial cellulose microfibers in Boops boops from the northern coasts of Sicily (Central Mediterranean)." Science of The Total Environment 691, no. : 455-465.

Journal article
Published: 28 October 2019 in Science of The Total Environment
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In the present work, the marine invertebrate Mytilus galloprovincialis was used as model organism to evaluate the toxic effects of the neonicotinoid Calypso 480 SC (CAL) following 20 days of exposure to sub-lethal concentrations of 7.77 mg L−1 (0.1% 96 h-LC50) and 77.70 mg L−1 (1% 96 h-LC50), and a recovery period of 10 days in uncontaminated seawater. Results revealed that exposure to both concentrations of CAL increased significantly mortality rate in the cells of haemolymph and digestive gland, while digestive gland cells were no longer able to regulate cell volume. Exposure significantly reduced haemolymph parameters (Cl−, Na+), affected the enzymatic activities of superoxide dismutase of digestive gland and catalase of gill, and caused also histopathological alterations in digestive gland and gills. Main histological damages detected in mussels were lipofuscin accumulation, focal points of necrosis, mucous overproduction and infiltrative inflammations. Interestingly, alterations persisted after the recovery period in CAL-free water, especially for haemocyte parameters (K+, Na+, Ca2+, lactate dehydrogenase, glucose). A slight recovery of histological conditions was detected. These findings suggested that sub-chronic exposure to the neonicotinoid insecticide caused significant alterations in both cell and tissue parameters of M. galloprovincialis. Considering the ecologically and commercially important role of mussels in coastal waters, a potential risk posed by neonicotinoids to this essential aquatic resource can be highlighted.

ACS Style

Alzbeta Stara; Maria Pagano; Gioele Capillo; Jacopo Fabrello; Marie Sandova; Irene Vazzana; Eliska Zuskova; Josef Velisek; Valerio Matozzo; Caterina Faggio. Assessing the effects of neonicotinoid insecticide on the bivalve mollusc Mytilus galloprovincialis. Science of The Total Environment 2019, 700, 134914 .

AMA Style

Alzbeta Stara, Maria Pagano, Gioele Capillo, Jacopo Fabrello, Marie Sandova, Irene Vazzana, Eliska Zuskova, Josef Velisek, Valerio Matozzo, Caterina Faggio. Assessing the effects of neonicotinoid insecticide on the bivalve mollusc Mytilus galloprovincialis. Science of The Total Environment. 2019; 700 ():134914.

Chicago/Turabian Style

Alzbeta Stara; Maria Pagano; Gioele Capillo; Jacopo Fabrello; Marie Sandova; Irene Vazzana; Eliska Zuskova; Josef Velisek; Valerio Matozzo; Caterina Faggio. 2019. "Assessing the effects of neonicotinoid insecticide on the bivalve mollusc Mytilus galloprovincialis." Science of The Total Environment 700, no. : 134914.

Journal article
Published: 28 June 2019 in Science of The Total Environment
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Vanadium accumulation levels in different tissues (muscle and organs) of the striped mullet Mugil cephalus (Linnaeus, 1758) and possible relationships with blood parameters were evaluated in a Natural Protected Area (Lake Faro, Sicily, Italy), during the winter of 2017. Hematological parameters (red blood cell, RBC; white blood cell, WBC; hemoglobin concentration, Hb; hematocrit, Hct; mean corpuscular volume, MCV; mean corpuscular hemoglobin, MCH; mean corpuscular hemoglobin concentration, MCHC; thrombocytes, TC), biometric indices (weight, total and fork length), and vanadium levels in muscles and organs (gills, liver, stomach and intestine) were determined. Statistical analyses showed significant differences in concentrations of vanadium of the analyzed tissue of M. cephalus and a positive relationship between vanadium concentration in the liver and some hematological parameters (RBC, Hb and Hct) and biometric indices. Our results underline the importance of fish blood parameters as sensitive indicators of toxic impact of environmental factors such as metals. This study, focusing on an ongoing topic, represents a valuable contribution to research concerning the monitoring and prevention of vanadium pollution in aquatic organisms and environments.

ACS Style

Francesco Fazio; Concetta Saoca; Marilena Sanfilippo; Gioele Capillo; Nunziacarla Spanò; Giuseppe Piccione. Response of vanadium bioaccumulation in tissues of Mugil cephalus (Linnaeus 1758). Science of The Total Environment 2019, 689, 774 -780.

AMA Style

Francesco Fazio, Concetta Saoca, Marilena Sanfilippo, Gioele Capillo, Nunziacarla Spanò, Giuseppe Piccione. Response of vanadium bioaccumulation in tissues of Mugil cephalus (Linnaeus 1758). Science of The Total Environment. 2019; 689 ():774-780.

Chicago/Turabian Style

Francesco Fazio; Concetta Saoca; Marilena Sanfilippo; Gioele Capillo; Nunziacarla Spanò; Giuseppe Piccione. 2019. "Response of vanadium bioaccumulation in tissues of Mugil cephalus (Linnaeus 1758)." Science of The Total Environment 689, no. : 774-780.

Journal article
Published: 01 April 2019 in Environmental Toxicology and Pharmacology
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In this work it is reported for the first time the characterization of microplastics from sea water samples and in two congener species of seabreams: Pagellus erythrinus and P. bogaraveo, Mediterranean fish species of great commercial importance. An experimental survey was conducted on May-June 2017 in the southernmost part of the Tyrrhenian Sea. Microplastics found in the sea water and in the grastrointestinal tract of two teleosts were characterized by Raman and IR spectroscopies. Microplastics found in sea water samples appeared in the form of fragments made of plastics of low and high density (PVC and LPDE). All the microplastics found in fish belonged to Nylon 66, typical fibers used in industry and in fisheries. Our findings highlighted the importance of further studies along the food web chain for a better understanding of the diffusion and possible consequences of this terrible threat.

ACS Style

Serena Savoca; Gioele Capillo; M. Mancuso; T. Bottari; Rosalia Crupi; C. Branca; Valentino Romano; C. Faggio; G. D’Angelo; N. Spanò. Microplastics occurrence in the Tyrrhenian waters and in the gastrointestinal tract of two congener species of seabreams. Environmental Toxicology and Pharmacology 2019, 67, 35 -41.

AMA Style

Serena Savoca, Gioele Capillo, M. Mancuso, T. Bottari, Rosalia Crupi, C. Branca, Valentino Romano, C. Faggio, G. D’Angelo, N. Spanò. Microplastics occurrence in the Tyrrhenian waters and in the gastrointestinal tract of two congener species of seabreams. Environmental Toxicology and Pharmacology. 2019; 67 ():35-41.

Chicago/Turabian Style

Serena Savoca; Gioele Capillo; M. Mancuso; T. Bottari; Rosalia Crupi; C. Branca; Valentino Romano; C. Faggio; G. D’Angelo; N. Spanò. 2019. "Microplastics occurrence in the Tyrrhenian waters and in the gastrointestinal tract of two congener species of seabreams." Environmental Toxicology and Pharmacology 67, no. : 35-41.

Journal article
Published: 17 March 2019 in Marine Drugs
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The phylum Cnidaria is one of the most important contributors in providing abundance of bio- and chemodiversity. In this study, a comprehensive chemical investigation on the nutritional and nutraceutical properties of Mediterranean jellyfish Pelagia noctiluca was carried out. Also, compositional differences between male and female organisms, as well as between their main anatomical parts, namely bell and oral arms, were explored in an attempt to select the best potential sources of nutrients and/or nutraceuticals from jellyfish. With the exception of higher energy densities and total phenolic contents observed in females than males, no statistically significant differences related to the specimen’s sex were highlighted for the other compound classes. Rather, the distribution of the investigated chemical classes varied depending on the jellyfish’s body parts. In fact, crude proteins were more abundant in oral arms than bells; saturated fatty acids were more concentrated in bells than oral arms, whereas polyunsaturated fatty acids were distributed in the exact opposite way. On the other hand, major elements and trace elements demonstrated an opposite behavior, being the latter most accumulated in oral arms than bells. Additionally, important nutraceuticals, such as eicosapentaenoic and docosahexaenoic acids, and antioxidant minerals, were determined. Overall, obtained data suggest the potential employment of the Mediterranean P. noctiluca for the development of natural aquafeed and food supplements.

ACS Style

Rosaria Costa; Gioele Capillo; Ambrogina Albergamo; Rosalia Li Volsi; Giovanni Bartolomeo; Giuseppe Bua; Antonio Ferracane; Serena Savoca; Teresa Gervasi; Rossana Rando; Giacomo Dugo; Nunziacarla Spanò. A Multi-screening Evaluation of the Nutritional and Nutraceutical Potential of the Mediterranean Jellyfish Pelagia noctiluca. Marine Drugs 2019, 17, 172 .

AMA Style

Rosaria Costa, Gioele Capillo, Ambrogina Albergamo, Rosalia Li Volsi, Giovanni Bartolomeo, Giuseppe Bua, Antonio Ferracane, Serena Savoca, Teresa Gervasi, Rossana Rando, Giacomo Dugo, Nunziacarla Spanò. A Multi-screening Evaluation of the Nutritional and Nutraceutical Potential of the Mediterranean Jellyfish Pelagia noctiluca. Marine Drugs. 2019; 17 (3):172.

Chicago/Turabian Style

Rosaria Costa; Gioele Capillo; Ambrogina Albergamo; Rosalia Li Volsi; Giovanni Bartolomeo; Giuseppe Bua; Antonio Ferracane; Serena Savoca; Teresa Gervasi; Rossana Rando; Giacomo Dugo; Nunziacarla Spanò. 2019. "A Multi-screening Evaluation of the Nutritional and Nutraceutical Potential of the Mediterranean Jellyfish Pelagia noctiluca." Marine Drugs 17, no. 3: 172.

Research article
Published: 02 February 2019 in Environmental Science and Pollution Research
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The authors investigated the arsenic (As) accumulation in different tissues (muscle, gill, liver, stomach, and intestine) and the possible correlation between tissue concentration and hematological parameters in mullet (Mugil cephalus Linnaeus, 1758) caught in Faro Lake (Messina, Sicily, Italy). On all fish, hematological analyses of blood samples, measurement of biometric indices, and the removal of the muscles, gills, liver, stomach, and intestine for the determination of arsenic concentration were performed. A hemogram was performed to find effects of arsenic concentration in tissues on hematological variables. One-way analysis of variance showed significant differences of arsenic concentration in different tissues, with higher values in the gill. The correlation between hematological parameters and tissue arsenic concentration showed a statistical significance for red blood cell (RBC), hemoglobin concentration (Hb), and hematocrit (Hct) with the liver As concentration. Biometric indices (weight, length, and fork length) showed a significant correlation with As concentration of the muscle and liver also. Our results indicate the role of some hematological parameters as biomarkers useful to monitoring anthropogenic load of arsenic in water and sediment, because variations of these parameters represent one of the effects that arsenic exposure can have on fish.

ACS Style

Francesco Fazio; Concetta Saoca; Vincenzo Ferrantelli; Gaetano Cammilleri; Gioele Capillo; Giuseppe Piccione. Relationship between arsenic accumulation in tissues and hematological parameters in mullet caught in Faro Lake: a preliminary study. Environmental Science and Pollution Research 2019, 26, 8821 -8827.

AMA Style

Francesco Fazio, Concetta Saoca, Vincenzo Ferrantelli, Gaetano Cammilleri, Gioele Capillo, Giuseppe Piccione. Relationship between arsenic accumulation in tissues and hematological parameters in mullet caught in Faro Lake: a preliminary study. Environmental Science and Pollution Research. 2019; 26 (9):8821-8827.

Chicago/Turabian Style

Francesco Fazio; Concetta Saoca; Vincenzo Ferrantelli; Gaetano Cammilleri; Gioele Capillo; Giuseppe Piccione. 2019. "Relationship between arsenic accumulation in tissues and hematological parameters in mullet caught in Faro Lake: a preliminary study." Environmental Science and Pollution Research 26, no. 9: 8821-8827.

Journal article
Published: 31 January 2019 in Fish & Shellfish Immunology
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The present study describes histochemical and immunohistochemical characteristics of the spiral valve and its associated lymphoid tissue (GALT) in the dogfish Scyliorhinus canicula. The mucosal surface of the spiral valve represents the first line of defense against pathogens coming from the external environment through food. Epithelial, mucus and immune cells play a key role in controlling the inflammatory response. Valve intestine of S. canicula had many folds lined by simple columnar cells and goblet cells, which later reacted positive to PAS, AB and AB-PAS, histochemical stains differentiated the different types of mucins; lectin histochemistry (PNA and WGA), detected neutral and acid mucins secreted that plays an important role in protection against invading pathogens. Integrin α5β1 was expressed in enterocytes that line the valve's folds with greater marking in the apical part of the cells. Laminin was found on the apical side of the epithelium, in fibrillar and cellular elements of the lamina propria and in the muscularis mucosa. In the spiral valve gut-associated lymphoid tissue (GALT) has been studied. For the first time, massive leucocytes aggregates were identified by confocal immunofluorescence techniques, using the following antibodies: TLR2, S100, Langerin/CD207. Our results expand knowledge about Dogfish valve intestine giving important news in understanding comparative immunology.

ACS Style

E.R. Lauriano; S. Pergolizzi; M. Aragona; G. Montalbano; M.C. Guerrera; R. Crupi; C. Faggio; G. Capillo. Intestinal immunity of dogfish Scyliorhinus canicula spiral valve: A histochemical, immunohistochemical and confocal study. Fish & Shellfish Immunology 2019, 87, 490 -498.

AMA Style

E.R. Lauriano, S. Pergolizzi, M. Aragona, G. Montalbano, M.C. Guerrera, R. Crupi, C. Faggio, G. Capillo. Intestinal immunity of dogfish Scyliorhinus canicula spiral valve: A histochemical, immunohistochemical and confocal study. Fish & Shellfish Immunology. 2019; 87 ():490-498.

Chicago/Turabian Style

E.R. Lauriano; S. Pergolizzi; M. Aragona; G. Montalbano; M.C. Guerrera; R. Crupi; C. Faggio; G. Capillo. 2019. "Intestinal immunity of dogfish Scyliorhinus canicula spiral valve: A histochemical, immunohistochemical and confocal study." Fish & Shellfish Immunology 87, no. : 490-498.

Journal article
Published: 07 December 2018 in Marine Drugs
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Enormous marine biodiversity offers an endless reservoir of chemicals for many applications. In this scenario, the extraction of seaweeds represents an interesting source of compounds displaying antimicrobial activity. In particular, among the different red algae, Gracilaria gracilis plays an important role due to the presence of important bioactives in its composition. In spite of these features, an efficient culture system is still absent. In the present study, a novel algal culture method was developed and compared to another more common cultural practice, widely reported in literature. A higher efficiency of the new method, both for daily growth rate and biomass, was assessed. Furthermore, the growth inhibitory activity of five extracts, obtained using ethanol, methanol, acetone, chloroform or diethyl ether as a solvent, from the cultured G. gracilis was tested against Gram-positive and Gram-negative pathogens. Algal extracts exhibited a considerable inhibitory activity against B. subtilis strains, while a slight inhibition was observed against V. fischeri. The different extracts showed significant differences in bacterial growth inhibition, with the highest activity that was recorded for the ethanol extract, followed by that of methanol. Based on the chemical characterization, these findings could be related to the antimicrobial activity played by the combination of total carbohydrates and polyphenols, which were determined at high levels in ethanol and methanol extracts, as well as by the highest number and levels of single polyphenols. Conversely, the lower growth inhibitory activities found in chloroform and diethyl ether extracts could be related to the isolation of minor lipid classes (e.g., neutral and medium polar lipids) composed by fatty acids, such as stearic, oleic and arachidonic acids, typically characterized by antimicrobial activity. In consideration of the results obtained, the present study has a double implication, involving both the field of cultural practices and the exploitation of natural sources for the isolation of antimicrobial agents useful both in pharmaceutical and food applications.

ACS Style

Gioele Capillo; Serena Savoca; Rosaria Costa; Marilena Sanfilippo; Carmen Rizzo; Angelina Lo Giudice; Ambrogina Albergamo; Rossana Rando; Giovanni Bartolomeo; Nunziacarla Spanò; Caterina Faggio. New Insights into the Culture Method and Antibacterial Potential of Gracilaria gracilis. Marine Drugs 2018, 16, 492 .

AMA Style

Gioele Capillo, Serena Savoca, Rosaria Costa, Marilena Sanfilippo, Carmen Rizzo, Angelina Lo Giudice, Ambrogina Albergamo, Rossana Rando, Giovanni Bartolomeo, Nunziacarla Spanò, Caterina Faggio. New Insights into the Culture Method and Antibacterial Potential of Gracilaria gracilis. Marine Drugs. 2018; 16 (12):492.

Chicago/Turabian Style

Gioele Capillo; Serena Savoca; Rosaria Costa; Marilena Sanfilippo; Carmen Rizzo; Angelina Lo Giudice; Ambrogina Albergamo; Rossana Rando; Giovanni Bartolomeo; Nunziacarla Spanò; Caterina Faggio. 2018. "New Insights into the Culture Method and Antibacterial Potential of Gracilaria gracilis." Marine Drugs 16, no. 12: 492.