This page has only limited features, please log in for full access.
Due to their beneficial properties, the use of zinc oxide nanoparticles (ZnO NP) is constantly increasing, especially in consumer-related areas, such as food packaging and food additives, which is leading to an increased oral uptake of ZnO NP. Consequently, the aim of our study was to investigate the cellular uptake of two differently sized ZnO NP (<50 nm and <100 nm; 12–1229 µmol/L) using two human intestinal cell lines (Caco-2 and LT97) and to examine the possible resulting toxic effects. ZnO NP (<50 nm and <100 nm) were internalized by both cell lines and led to intracellular changes. Both ZnO NP caused time- and dose-dependent cytotoxic effects, especially at concentrations of 614 µmol/L and 1229 µmol/L, which was associated with an increased rate of apoptotic and dead cells. ZnO NP < 100 nm altered the cell cycle of LT97 cells but not that of Caco-2 cells. ZnO NP < 50 nm led to the formation of micronuclei in LT97 cells. The Ames test revealed no mutagenicity for both ZnO NP. Our results indicate the potential toxicity of ZnO NP after oral exposure, which should be considered before application.
Anna Mittag; Christian Hoera; Alexander Kämpfe; Martin Westermann; Jochen Kuckelkorn; Thomas Schneider; Michael Glei. Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells. Toxics 2021, 9, 96 .
AMA StyleAnna Mittag, Christian Hoera, Alexander Kämpfe, Martin Westermann, Jochen Kuckelkorn, Thomas Schneider, Michael Glei. Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells. Toxics. 2021; 9 (5):96.
Chicago/Turabian StyleAnna Mittag; Christian Hoera; Alexander Kämpfe; Martin Westermann; Jochen Kuckelkorn; Thomas Schneider; Michael Glei. 2021. "Cellular Uptake and Toxicological Effects of Differently Sized Zinc Oxide Nanoparticles in Intestinal Cells." Toxics 9, no. 5: 96.
Nanoparticles (NPs) are increasingly used in different consumer-related areas, for instance in food packaging or as additives, because of their enormous potential. Magnesium oxide (MgO) is an EU-approved food additive (E number 530). It is commonly used as a drying agent for powdered foods, for colour retention or as a food supplement. There are no consistent results regarding the effects of oral MgO NP uptake. Consequently, the aim of this study was to examine the effects of MgO NPs in the HT29 intestinal cell line. MgO NP concentrations ranged from 0.001 to 100 μg/ml and incubation times were up to 24 h. The cytotoxic and genotoxic potential were investigated. Apoptotic processes and cell cycle changes were analysed by flow cytometry. Finally, oxidative stress was examined. Transmission electron microscopy indicated that there was no cellular uptake. MgO NPs had no cytotoxic or genotoxic effects in HT29 cells and they did not induce apoptotic processes, cell cycle changes or oxidative stress.
Anna Mittag; Thomas Schneider; Martin Westermann; Michael Glei. Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells. Archives of Toxicology 2019, 93, 1491 -1500.
AMA StyleAnna Mittag, Thomas Schneider, Martin Westermann, Michael Glei. Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells. Archives of Toxicology. 2019; 93 (6):1491-1500.
Chicago/Turabian StyleAnna Mittag; Thomas Schneider; Martin Westermann; Michael Glei. 2019. "Toxicological assessment of magnesium oxide nanoparticles in HT29 intestinal cells." Archives of Toxicology 93, no. 6: 1491-1500.