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Dr. Pradeep Pushpakumara
Scientist

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0 Cell Culture
0 biochemestry, genetics and molecular biology
0 Bioinformatics & Biotechnology
0 Viral antibodies
0 Immuno-assays

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Journal article
Published: 24 May 2021 in Viruses
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Cross-reactive T cell immunity to seasonal coronaviruses (HCoVs) may lead to immunopathology or protection during SARS-CoV2 infection. To understand the influence of cross-reactive T cell responses, we used IEDB (Immune epitope database) and NetMHCpan (ver. 4.1) to identify candidate CD8+ T cell epitopes, restricted through HLA-A and B alleles. Conservation analysis was carried out for these epitopes with HCoVs, OC43, HKU1, and NL63. 12/18 the candidate CD8+ T cell epitopes (binding score of ≥0.90), which had a high degree of homology (>75%) with the other three HCoVs were within the NSP12 and NSP13 proteins. They were predicted to be restricted through HLA-A*2402, HLA-A*201, HLA-A*206, and HLA-B alleles B*3501. Thirty-one candidate CD8+ T cell epitopes that were specific to SARS-CoV2 virus (<25% homology with other HCoVs) were predominantly identified within the structural proteins (spike, envelop, membrane, and nucleocapsid) and the NSP1, NSP2, and NSP3. They were predominantly restricted through HLA-B*3501 (6/31), HLA-B*4001 (6/31), HLA-B*4403 (7/31), and HLA-A*2402 (8/31). It would be crucial to understand T cell responses that associate with protection, and the differences in the functionality and phenotype of epitope specific T cell responses, presented through different HLA alleles common in different geographical groups, to understand disease pathogenesis.

ACS Style

Pradeep Pushpakumara; Deshan Madhusanka; Saubhagya Dhanasekara; Chandima Jeewandara; Graham Ogg; Gathsaurie Malavige. Identification of Novel Candidate CD8+ T Cell Epitopes of the SARS-CoV2 with Homology to Other Seasonal Coronaviruses. Viruses 2021, 13, 972 .

AMA Style

Pradeep Pushpakumara, Deshan Madhusanka, Saubhagya Dhanasekara, Chandima Jeewandara, Graham Ogg, Gathsaurie Malavige. Identification of Novel Candidate CD8+ T Cell Epitopes of the SARS-CoV2 with Homology to Other Seasonal Coronaviruses. Viruses. 2021; 13 (6):972.

Chicago/Turabian Style

Pradeep Pushpakumara; Deshan Madhusanka; Saubhagya Dhanasekara; Chandima Jeewandara; Graham Ogg; Gathsaurie Malavige. 2021. "Identification of Novel Candidate CD8+ T Cell Epitopes of the SARS-CoV2 with Homology to Other Seasonal Coronaviruses." Viruses 13, no. 6: 972.