This page has only limited features, please log in for full access.

Dr. Vladimir Lukin
None

Basic Info

Basic Info is private.

Research Keywords & Expertise

0 Hypothesis Testing
0 Machine Learning
0 Viruses
0 statistical visual learning
0 Statistical analyses

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 12 May 2021 in Viruses
Reads 0
Downloads 0

As the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic expanded, it was clear that effective testing for the presence of neutralizing antibodies in the blood of convalescent patients would be critical for development of plasma-based therapeutic approaches. To address the need for a high-quality neutralization assay against SARS-CoV-2, a previously established fluorescence reduction neutralization assay (FRNA) against Middle East respiratory syndrome coronavirus (MERS-CoV) was modified and optimized. The SARS-CoV-2 FRNA provides a quantitative assessment of a large number of infected cells through use of a high-content imaging system. Because of this approach, and the fact that it does not involve subjective interpretation, this assay is more efficient and more accurate than other neutralization assays. In addition, the ability to set robust acceptance criteria for individual plates and specific test wells provided further rigor to this assay. Such agile adaptability avails use with multiple virus variants. By February 2021, the SARS-CoV-2 FRNA had been used to screen over 5000 samples, including acute and convalescent plasma or serum samples and therapeutic antibody treatments, for SARS-CoV-2 neutralizing titers.

ACS Style

Richard Bennett; Elena Postnikova; Janie Liang; Robin Gross; Steven Mazur; Saurabh Dixit; Gregory Kocher; Shuiqing Yu; Shalamar Georgia-Clark; Dawn Gerhardt; Yingyun Cai; Lindsay Marron; Vladimir Lukin; Michael Holbrook. Scalable, Micro-Neutralization Assay for Assessment of SARS-CoV-2 (COVID-19) Virus-Neutralizing Antibodies in Human Clinical Samples. Viruses 2021, 13, 893 .

AMA Style

Richard Bennett, Elena Postnikova, Janie Liang, Robin Gross, Steven Mazur, Saurabh Dixit, Gregory Kocher, Shuiqing Yu, Shalamar Georgia-Clark, Dawn Gerhardt, Yingyun Cai, Lindsay Marron, Vladimir Lukin, Michael Holbrook. Scalable, Micro-Neutralization Assay for Assessment of SARS-CoV-2 (COVID-19) Virus-Neutralizing Antibodies in Human Clinical Samples. Viruses. 2021; 13 (5):893.

Chicago/Turabian Style

Richard Bennett; Elena Postnikova; Janie Liang; Robin Gross; Steven Mazur; Saurabh Dixit; Gregory Kocher; Shuiqing Yu; Shalamar Georgia-Clark; Dawn Gerhardt; Yingyun Cai; Lindsay Marron; Vladimir Lukin; Michael Holbrook. 2021. "Scalable, Micro-Neutralization Assay for Assessment of SARS-CoV-2 (COVID-19) Virus-Neutralizing Antibodies in Human Clinical Samples." Viruses 13, no. 5: 893.