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The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.
Abdul Ajmal; Saleha Saroosh; Shah Mulk; Muhammad Hassan; Humaira Yasmin; Zahra Jabeen; Asia Nosheen; Syed Shah; Rabia Naz; Zuhair Hasnain; Tariq Qureshi; Abdul Waheed; Saqib Mumtaz. Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits. Sustainability 2021, 13, 7792 .
AMA StyleAbdul Ajmal, Saleha Saroosh, Shah Mulk, Muhammad Hassan, Humaira Yasmin, Zahra Jabeen, Asia Nosheen, Syed Shah, Rabia Naz, Zuhair Hasnain, Tariq Qureshi, Abdul Waheed, Saqib Mumtaz. Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits. Sustainability. 2021; 13 (14):7792.
Chicago/Turabian StyleAbdul Ajmal; Saleha Saroosh; Shah Mulk; Muhammad Hassan; Humaira Yasmin; Zahra Jabeen; Asia Nosheen; Syed Shah; Rabia Naz; Zuhair Hasnain; Tariq Qureshi; Abdul Waheed; Saqib Mumtaz. 2021. "Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits." Sustainability 13, no. 14: 7792.
The study investigated the green and chemical approaches for the preparation of Zn nanoparticles and their effect on the growth of okra plants under saline conditions. The leaf extract of Sorghum bicolor L. was used for the green synthesis of zinc nanoparticles (Zn-GNPs). Zinc nanoparticles (Zn-NPs) were also produced by the co-precipitation method (Zn-CNPs). The synthesized NPs were characterized by UV-visible spectroscopy, X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR) and were applied foliarly in the range of 0.1%, 0.2%, 0.3% on okra plants. A marked increase in the shoot and root fresh and dry weight (g) and chlorophyll contents were observed under normal and saline conditions. An increase in antioxidant activity was observed under saline conditions. However, the foliar application of 0.3% Zn-GNPs was helpful in the regulation of the antioxidant defense system under a saline environment. Based on the results, it can be concluded that the use of Zn-GNPs is the most promising eco-friendly approach in mitigating salinity stress.
Sara Zafar; Zuhair Hasnain; Nazia Aslam; Saqib Mumtaz; Hawa Jaafar; Puteri Wahab; Mughal Qayum; Alexe Ormenisan. Impact of Zn Nanoparticles Synthesized via Green and Chemical Approach on Okra (Abelmoschus esculentus L.) Growth under Salt Stress. Sustainability 2021, 13, 3694 .
AMA StyleSara Zafar, Zuhair Hasnain, Nazia Aslam, Saqib Mumtaz, Hawa Jaafar, Puteri Wahab, Mughal Qayum, Alexe Ormenisan. Impact of Zn Nanoparticles Synthesized via Green and Chemical Approach on Okra (Abelmoschus esculentus L.) Growth under Salt Stress. Sustainability. 2021; 13 (7):3694.
Chicago/Turabian StyleSara Zafar; Zuhair Hasnain; Nazia Aslam; Saqib Mumtaz; Hawa Jaafar; Puteri Wahab; Mughal Qayum; Alexe Ormenisan. 2021. "Impact of Zn Nanoparticles Synthesized via Green and Chemical Approach on Okra (Abelmoschus esculentus L.) Growth under Salt Stress." Sustainability 13, no. 7: 3694.
Fusarium root and crown rot is the most common disease of wheat, especially wheat grown in arid zones where drought is a common issue. The development of environmentally safe approaches to manage diseases of food crops is important for humans. The monocropping system recruits beneficial bacteria that promote plant growth through nutrient solubilization and pathogen suppression. In this study, a field where wheat was monocropped for 5 successive years under rainfed conditions was identified. A total of 29 bacterial isolates were obtained from the rhizosphere, endosphere, and phyllosphere of wheat at its harvesting stage. The Gram-negative bacteria were less prevalent (41%) but the majority (75%) exhibited plant growth-promoting traits. The ability of strains to solubilize nutrients (solubilization index = 2.3 to 4), inhibit pathogenic fungi (25 to 56%), and produce antifungal compounds was highly variable. The rhizobacteria significantly promoted the growth and disease resistance of wheat varieties such as Pirsbak-2015 and Galaxy-2013 by inducing antioxidant enzyme activity (0.2- to 2.1-fold). The bacterial strains were identified as Ochrobactrum spp., Acinetobacter spp., and Pseudomonas mediterranea by 16S rRNA and rpoD sequence analysis. The endophytic bacterium P. mediterranea HU-9 exhibited maximum biocontrol efficacy against wheat root and crown rot diseases with a disease score/disease index from 1.8 to 3.1. The monocropping systems of rainfed agriculture are an ideal source of beneficial bacteria to use as bioinoculants for different crops.
Habib Ullah; Humaira Yasmin; Saqib Mumtaz; Zahra Jabeen; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot. Phytopathology® 2020, 110, 582 -592.
AMA StyleHabib Ullah, Humaira Yasmin, Saqib Mumtaz, Zahra Jabeen, Rabia Naz, Asia Nosheen, Muhammad Nadeem Hassan. Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot. Phytopathology®. 2020; 110 (3):582-592.
Chicago/Turabian StyleHabib Ullah; Humaira Yasmin; Saqib Mumtaz; Zahra Jabeen; Rabia Naz; Asia Nosheen; Muhammad Nadeem Hassan. 2020. "Multitrait Pseudomonas spp. Isolated from Monocropped Wheat (Triticum aestivum) Suppress Fusarium Root and Crown Rot." Phytopathology® 110, no. 3: 582-592.
Soil microorganisms may respond to metal stress by a shift in the microbial community from metal sensitive to metal resistant microorganisms. We assessed the bacterial community from low (2-20 mg kg), medium (200-400 mg kg), high (500-900 mg kg) and very high (>900 mg kg) uranium soils at Ranger Uranium Mine in northern Australia through pyrosequencing. Proteobacteria (28.85%) was the most abundant phylum at these sites, followed by Actinobacteria (9.31%), Acidobacteria (7.33%), Verrucomicrobia (2.11%), Firmicutes (2.02%), Chloroflexi (1.11%), Cyanobacteria (0.93%), Planctomycetes (0.82%), Bacteroidetes (0.46%) and Candidate_division_WS3 (Latescibacteria) (0.21%). However, 46.79% of bacteria were unclassified. Bacteria at low U soils differed from soils with elevated uranium. Bacterial OTUs closely related to Kitasatospora spp., Sphingobacteria spp. and Rhodobium spp. were only present at higher uranium concentrations and the bacterial community also changed with seasonal and temporal changes in soil uranium and physicochemical variables. This study using next generation sequencing in association with environmental variables at a uranium mine has laid a foundation for further studies of soil-microbe-metal interactions which may be useful for developing sustainable management and rehabilitation strategies. Furthermore, bacterial species associated with higher uranium may serve as useful indicators of uranium contamination in the wet-dry tropics.
Saqib Mumtaz; Claire Streten; David L. Parry; Keith A. McGuinness; Ping Lu; Karen S. Gibb. Soil uranium concentration at Ranger Uranium Mine Land Application Areas drives changes in the bacterial community. Journal of Environmental Radioactivity 2018, 189, 14 -23.
AMA StyleSaqib Mumtaz, Claire Streten, David L. Parry, Keith A. McGuinness, Ping Lu, Karen S. Gibb. Soil uranium concentration at Ranger Uranium Mine Land Application Areas drives changes in the bacterial community. Journal of Environmental Radioactivity. 2018; 189 ():14-23.
Chicago/Turabian StyleSaqib Mumtaz; Claire Streten; David L. Parry; Keith A. McGuinness; Ping Lu; Karen S. Gibb. 2018. "Soil uranium concentration at Ranger Uranium Mine Land Application Areas drives changes in the bacterial community." Journal of Environmental Radioactivity 189, no. : 14-23.
Ranger Uranium Mine (RUM) is situated in the wet-dry tropics of Northern Australia. Land application (irrigation) of stockpile (ore and waste) runoff water to natural woodland on the mine lease is a key part of water management at the mine. Consequently, the soil in these Land Application Areas (LAAs) presents a range of uranium (U) and other metals concentrations. Knowledge of seasonal and temporal changes in soil U and physicochemical parameters at RUM LAAs is important to develop suitable management and rehabilitation strategies. Therefore, soil samples were collected from low, medium, high and very high U sites at RUM LAAs for two consecutive years and the effect of time and season on soil physicochemical parameters particularly U and other major solutes applied in irrigation water was measured. Concentrations of some of the solutes applied in the irrigation water such as sulphur (S), iron (Fe) and calcium (Ca) showed significant seasonal and temporal changes. Soil S, Fe and Ca concentration decreased from year 1 to year 2 and from dry to wet seasons during both years. Soil U followed the same pattern except that we recorded an increase in soil U concentrations at most of the RUM LAAs after year 2 wet season compared to year 2 dry season. Thus, these sites did not show a considerable decrease in soil U concentration from year 1 to year 2. Sites which contained elevated U after wet season 2 also had higher moisture content which suggests that pooling of U containing rainwater at these sites may be responsible for elevated U. Thus, U may be redistributed within RUM LAAs due to surface water movement. The study also suggested that a decrease in U concentrations in LAA soils at very high U (>900 mg kg(-1)) sites is most likely due to transport of particulate matter bound U by surface runoff and U may not be lost from the surface soil due to vertical movement through the soil profile. Uranium attached to particulate matter may reduce its potential for environmental impact. These findings suggest that U is effectively adsorbed by the soils and thus land application may serve as a useful tool for U management in the wet-dry tropics of northern Australia.
Saqib Mumtaz; Claire Streten; David L. Parry; Keith A. McGuinness; Ping Lu; Karen S. Gibb. Land application of mine water causes minimal uranium loss offsite in the wet-dry tropics: Ranger Uranium Mine, Northern Territory, Australia. Journal of Environmental Radioactivity 2015, 149, 121 -128.
AMA StyleSaqib Mumtaz, Claire Streten, David L. Parry, Keith A. McGuinness, Ping Lu, Karen S. Gibb. Land application of mine water causes minimal uranium loss offsite in the wet-dry tropics: Ranger Uranium Mine, Northern Territory, Australia. Journal of Environmental Radioactivity. 2015; 149 ():121-128.
Chicago/Turabian StyleSaqib Mumtaz; Claire Streten; David L. Parry; Keith A. McGuinness; Ping Lu; Karen S. Gibb. 2015. "Land application of mine water causes minimal uranium loss offsite in the wet-dry tropics: Ranger Uranium Mine, Northern Territory, Australia." Journal of Environmental Radioactivity 149, no. : 121-128.