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Post-doctoral scientist working at the Pomeranian Medical University in Szczecin
The occurrence of HIV-1 subtypes differs worldwide and within Europe, with non-B variants mainly found across different exposure groups. In this study, we investigated the distribution and temporal trends in HIV-1 subtype variability across Poland between 2015 and 2019. Sequences of the pol gene fragment from 2518 individuals were used for the analysis of subtype prevalence. Subtype B was dominant (n = 2163, 85.90%). The proportion of subtype B-infected individuals decreased significantly, from 89.3% in 2015 to 80.3% in 2019. This was related to the increasing number of subtype A infections. In 355 (14.10%) sequences, non-B variants were identified. In 65 (2.58%) samples, recombinant forms (RFs) were noted. Unique recombinant forms (URFs) were found in 30 (1.19%) sequences. Three A/B recombinant clusters were identified of which two were A6/B mosaic viruses not previously described. Non-B clades were significantly more common among females (n = 81, 22.8%, p = 0.001) and heterosexually infected individuals (n = 45, 32.4%, p = 0.0031). The predominance of subtype B is evident, but the variability of HIV-1 in Poland is notable. Almost half of RFs (n = 65, 2.58%) was comprised of URFs (n = 30, 1.19%); thus those forms were common in the analyzed population. Hence, molecular surveillance of identified variants ensures recognition of HIV-1 evolution in Poland.
Karol Serwin; Anna Urbańska; Kaja Scheibe; Magdalena Witak-Jędra; Maria Jankowska; Maria Hlebowicz; Monika Bociąga-Jasik; Anna Kalinowska-Nowak; Martyna Biała; Hubert Ciepłucha; Władysław Łojewski; Anita Olczak; Elżbieta Jabłonowska; Aldona Kowalczuk-Kot; Błażej Rozpłochowski; Adam Witor; Miłosz Parczewski. Molecular epidemiology and HIV-1 variant evolution in Poland between 2015 and 2019. Scientific Reports 2021, 11, 1 -17.
AMA StyleKarol Serwin, Anna Urbańska, Kaja Scheibe, Magdalena Witak-Jędra, Maria Jankowska, Maria Hlebowicz, Monika Bociąga-Jasik, Anna Kalinowska-Nowak, Martyna Biała, Hubert Ciepłucha, Władysław Łojewski, Anita Olczak, Elżbieta Jabłonowska, Aldona Kowalczuk-Kot, Błażej Rozpłochowski, Adam Witor, Miłosz Parczewski. Molecular epidemiology and HIV-1 variant evolution in Poland between 2015 and 2019. Scientific Reports. 2021; 11 (1):1-17.
Chicago/Turabian StyleKarol Serwin; Anna Urbańska; Kaja Scheibe; Magdalena Witak-Jędra; Maria Jankowska; Maria Hlebowicz; Monika Bociąga-Jasik; Anna Kalinowska-Nowak; Martyna Biała; Hubert Ciepłucha; Władysław Łojewski; Anita Olczak; Elżbieta Jabłonowska; Aldona Kowalczuk-Kot; Błażej Rozpłochowski; Adam Witor; Miłosz Parczewski. 2021. "Molecular epidemiology and HIV-1 variant evolution in Poland between 2015 and 2019." Scientific Reports 11, no. 1: 1-17.
The emergence of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) evolved into a worldwide outbreak, with the first Polish cases in February/March 2020. This study aimed to investigate the molecular epidemiology of the circulating virus lineages between March 2020 and February 2021. We performed variant identification, spike mutation pattern analysis, and phylogenetic and evolutionary analyses for 1106 high-coverage whole-genome sequences, implementing maximum likelihood, multiple continuous-time Markov chain, and Bayesian birth–death skyline models. For time trends, logistic regression was used. In the dataset, virus B.1.221 lineage was predominant (15.37%), followed by B.1.258 (15.01%) and B.1.1.29 (11.48%) strains. Three clades were identified, being responsible for 74.41% of infections over the analyzed period. Expansion in variant diversity was observed since September 2020 with increasing frequency of the number in spike substitutions, mainly H69V70 deletion, P681H, N439K, and S98F. In population dynamics inferences, three periods with exponential increase in infection were observed, beginning in March, July, and September 2020, respectively, and were driven by different virus clades. Additionally, a notable increase in infections caused by the B.1.1.7 lineage since February 2021 was noted. Over time, the virus accumulated mutations related to optimized transmissibility; therefore, faster dissemination is reflected by the second wave of epidemics in Poland.
Karol Serwin; Andrzej Ossowski; Maria Szargut; Sandra Cytacka; Anna Urbańska; Adam Majchrzak; Anna Niedźwiedź; Ewa Czerska; Anna Pawińska-Matecka; Joanna Gołąb; Miłosz Parczewski. Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland. Viruses 2021, 13, 1295 .
AMA StyleKarol Serwin, Andrzej Ossowski, Maria Szargut, Sandra Cytacka, Anna Urbańska, Adam Majchrzak, Anna Niedźwiedź, Ewa Czerska, Anna Pawińska-Matecka, Joanna Gołąb, Miłosz Parczewski. Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland. Viruses. 2021; 13 (7):1295.
Chicago/Turabian StyleKarol Serwin; Andrzej Ossowski; Maria Szargut; Sandra Cytacka; Anna Urbańska; Adam Majchrzak; Anna Niedźwiedź; Ewa Czerska; Anna Pawińska-Matecka; Joanna Gołąb; Miłosz Parczewski. 2021. "Molecular Evolution and Epidemiological Characteristics of SARS COV-2 in (Northwestern) Poland." Viruses 13, no. 7: 1295.
Diagnosis of kidney diseases has recently become more comprehensive and accurate by using new renal markers. Despite the fact that creatinine and cystatin c have been sufficient in determining kidney function, they did not indicate the exact site of the damage and they were often insufficient in predicting the course of the disease. Aim of the study was to evaluate the potential correlations and differences in levels of six factors related to kidney function and injury: kidney injury molecule-1 (KIM-1), ncalbindin (CALB), glutathione S-transferase Pi (GST-Pi), calbindin and monocyte chemoattractant protein-1 (MCP-1), between renal patients with diabetic nephropathy (DM), congenital defects (CD) of the kidney and glomerulonephritis (GN). Study involved 75 patients: 49 with diabetic nephropathy, 12 with congenital defects and 14 with glomerulonephritis. Levels of renalase was measured using immunoenzymatic tests. Levels of other markers: calbindin, glutathione-S-transferase (GST-pi), interleukin-18 (IL-18), kidney injury molecule-1 (KIM-1) and monocyte chemoattractant protein-1 (MCP-1), were analyzed using Kidney Toxicity-1 Panel and BioPlex system, designed for analyses in urine and optimized by us for serum.From all analyzed markers, only levels of KIM-1 differed significantly between any subgroups, and that was for CD and DM. Renalase correlated significantly negatively with creatinine and positively with all other markers, apart from MCP-1. Obtained results indicate, that serum renalase, KIM-1, calbindin and GST-pi are related to kidney function, with KIM-1 being the most exact, while MCP-1 levels are unrelated to creatinine and glucose levels, does not differ between patients with diabetic nephropathy and other subgroups, and therefore seem to be independent of diabetes. Also, serum-optimized Kidney Toxicity Panel 1 kit for determination of selected markers gave results similar to previous ones and therefore the method can be valuable in determination of analyzed factors.
Natalia Maria Serwin; Magda Wiśniewska; Edyta Skwirczyńska; Karol Serwin; Oskar Wróblewski; Barbara Dołegowska. Searching for Perfect Marker - Differences and Dependencies in Serum Levels of Renalase, KIM-1, MCP-1, Calbindin and GST-Pi in Patients with Diabetes, Glomerulonephritis and Congenital Defects of the Kidney. 2018, 1 .
AMA StyleNatalia Maria Serwin, Magda Wiśniewska, Edyta Skwirczyńska, Karol Serwin, Oskar Wróblewski, Barbara Dołegowska. Searching for Perfect Marker - Differences and Dependencies in Serum Levels of Renalase, KIM-1, MCP-1, Calbindin and GST-Pi in Patients with Diabetes, Glomerulonephritis and Congenital Defects of the Kidney. . 2018; ():1.
Chicago/Turabian StyleNatalia Maria Serwin; Magda Wiśniewska; Edyta Skwirczyńska; Karol Serwin; Oskar Wróblewski; Barbara Dołegowska. 2018. "Searching for Perfect Marker - Differences and Dependencies in Serum Levels of Renalase, KIM-1, MCP-1, Calbindin and GST-Pi in Patients with Diabetes, Glomerulonephritis and Congenital Defects of the Kidney." , no. : 1.
Summary Staphylococcus aureus is a dangerous human pathogen characterized by alarmingly increasing antibiotic resistance. Accumulating evidence suggests the role of Spl proteases in staphylococcal virulence. Spl proteases have restricted, non-overlapping substrate specificity, suggesting that they may constitute a first example of a proteolytic system in bacteria. SplA, SplB, and SplD were previously characterized in terms of substrate specificity and structural determinants thereof. Here we analyze the substrate specificity of SplE documenting its unique P1 preference among Spl proteases and, in fact, among all chymotrypsin-like (family S1) proteases characterized to date. This is interesting since our understanding of the general aspects of proteolysis is based on seminal studies of S1 family members. To better understand the molecular determinants of the unusual specificity of SplE, the crystal structure of the protein is determined here. Conclusions from structural analysis are evaluated by successful grafting of SplE specificity on the scaffold of SplB protease.
Natalia Stach; Magdalena Kalinska; Michal Zdzalik; Radoslaw Kitel; Abdulkarim Karim; Karol Serwin; Wioletta Rut; Katrine Larsen; Abeer Jabaiah; Magdalena Firlej; Benedykt Wladyka; Patrick Daugherty; Henning Stennicke; Marcin Drag; Jan Potempa; Grzegorz Dubin. Unique Substrate Specificity of SplE Serine Protease from Staphylococcus aureus. Structure 2018, 26, 572 -579.e4.
AMA StyleNatalia Stach, Magdalena Kalinska, Michal Zdzalik, Radoslaw Kitel, Abdulkarim Karim, Karol Serwin, Wioletta Rut, Katrine Larsen, Abeer Jabaiah, Magdalena Firlej, Benedykt Wladyka, Patrick Daugherty, Henning Stennicke, Marcin Drag, Jan Potempa, Grzegorz Dubin. Unique Substrate Specificity of SplE Serine Protease from Staphylococcus aureus. Structure. 2018; 26 (4):572-579.e4.
Chicago/Turabian StyleNatalia Stach; Magdalena Kalinska; Michal Zdzalik; Radoslaw Kitel; Abdulkarim Karim; Karol Serwin; Wioletta Rut; Katrine Larsen; Abeer Jabaiah; Magdalena Firlej; Benedykt Wladyka; Patrick Daugherty; Henning Stennicke; Marcin Drag; Jan Potempa; Grzegorz Dubin. 2018. "Unique Substrate Specificity of SplE Serine Protease from Staphylococcus aureus." Structure 26, no. 4: 572-579.e4.
W Marlicz; E Wunsch; M Mydlowska; Malgorzata Milkiewicz; Karol Serwin; M Mularczyk; Piotr Milkiewicz; J Raszeja-Wyszomirska. The effect of short term treatment with probiotic VSL#3 on various clinical and biochemical parameters in patients with liver cirrhosis. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2016, 67, 867 -877.
AMA StyleW Marlicz, E Wunsch, M Mydlowska, Malgorzata Milkiewicz, Karol Serwin, M Mularczyk, Piotr Milkiewicz, J Raszeja-Wyszomirska. The effect of short term treatment with probiotic VSL#3 on various clinical and biochemical parameters in patients with liver cirrhosis. Journal of physiology and pharmacology : an official journal of the Polish Physiological Society. 2016; 67 (6):867-877.
Chicago/Turabian StyleW Marlicz; E Wunsch; M Mydlowska; Malgorzata Milkiewicz; Karol Serwin; M Mularczyk; Piotr Milkiewicz; J Raszeja-Wyszomirska. 2016. "The effect of short term treatment with probiotic VSL#3 on various clinical and biochemical parameters in patients with liver cirrhosis." Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 67, no. 6: 867-877.
Diluted (1%) plasma induces migration of malignant cell lines much more strongly than potent pro-metastatic factors. To characterize the factor(s) present in diluted plasma responsible for this phenomenon we performed i) heat inactivation, ii) dialysis, iii) proteinase K treatment, and iv) molecular size filtration studies. We found that this remarkable pro-migratory activity of diluted normal plasma is associated with a ~50–100-kD protein that interacts with GαI protein-coupled receptors and activates p42/44 MAPK and AKT signaling in target cells. Since this pro-migratory activity of 1% plasma decreases at higher plasma concentrations (> 20%), but is retained in serum, we hypothesized that fibrinogen may be involved as a chaperone of the protein(s). To identify the pro-migratory protein(s) present in diluted plasma and fibrinogen-depleted serum, we performed gel filtration and hydrophobic interaction chromatography followed by mass spectrometry analysis. We identified several putative protein candidates that were further tested in in vitro experiments. We found that this pro-migratory factor chaperoned by fibrinogen is vitronectin, which activates uPAR, and that this effect can be inhibited by fibrinogen. These results provide a novel mechanism for the metastasis of cancer cells to lymphatics and body cavities, in which the concentration of fibrinogen is low, and thus suggests that free vitronectin stimulates migration of tumor cells.
Gabriela Schneider; Ewa Bryndza; Agata Poniewierska-Baran; Karol Serwin; Malwina Suszynska; Zachariah P. Sellers; Michael L. Merchant; Alagammai Kaliappan; Janina Ratajczak; Magda Kucia; Nichola C. Garbett; Mariusz Z. Ratajczak. Evidence that vitronectin is a potent migration-enhancing factor for cancer cells chaperoned by fibrinogen: a novel view of the metastasis of cancer cells to low-fibrinogen lymphatics and body cavities. Oncotarget 2016, 7, 69829 -69843.
AMA StyleGabriela Schneider, Ewa Bryndza, Agata Poniewierska-Baran, Karol Serwin, Malwina Suszynska, Zachariah P. Sellers, Michael L. Merchant, Alagammai Kaliappan, Janina Ratajczak, Magda Kucia, Nichola C. Garbett, Mariusz Z. Ratajczak. Evidence that vitronectin is a potent migration-enhancing factor for cancer cells chaperoned by fibrinogen: a novel view of the metastasis of cancer cells to low-fibrinogen lymphatics and body cavities. Oncotarget. 2016; 7 (43):69829-69843.
Chicago/Turabian StyleGabriela Schneider; Ewa Bryndza; Agata Poniewierska-Baran; Karol Serwin; Malwina Suszynska; Zachariah P. Sellers; Michael L. Merchant; Alagammai Kaliappan; Janina Ratajczak; Magda Kucia; Nichola C. Garbett; Mariusz Z. Ratajczak. 2016. "Evidence that vitronectin is a potent migration-enhancing factor for cancer cells chaperoned by fibrinogen: a novel view of the metastasis of cancer cells to low-fibrinogen lymphatics and body cavities." Oncotarget 7, no. 43: 69829-69843.
Background. One of the crucial problems with cancer therapy is migration of cancer cells that leave primary tumor and migrate to vital organs where they form metastases. Throughout the years several factors have been identified that direct metastatic process including growth factors, chemokines, bioactive lipids or extracellular nucleotides. To our surprise however, we noticed that highly diluted plasma (1%) possess remarkable chemokinetic activity against several cancer cell lines that highly exceeds those observed for optimal doses of chemokines (e.g. SDF-1) and growth factors (e.g. HGF/SF), that are considered as a main metastatic factors present in plasma. Aim of the study. Based on this observation our aim was to identify the “factor/s” responsible for this remarkable chemokinetic activity of normal diluted 1% plasma. Experimental strategies. We employed several human cancer cell lines and different dilutions of normal human, murine and bovine plasma and serum in Transwell migration assays, adhesion and cell signaling studies. We tested effect of heat inactivation, protease exposure, dialysis and molecular filtration on chemotactic activity of plasma. We also used gel filtration followed by hydrophobic interaction chromatography (HIC) to identified plasma fractions with the most potent chemotactic activity that were further analyze using MassSpec. Results. We found that remarkable chemokinetic activity of highly diluted 1% plasma against malignant cells, rapidly decreased at higher plasma concentration (>5%). Our initial characterization studies revealed that this “activity” is sensitive to proteolytic treatment, is not removed from plasma by dialysis and is temperature sensitive. The similar effect has been observed for diluted 1% serum however chemotactic responsiveness of serum was maintained with its higher concentrations. Based on this we hypothesized possible involvement of inhibitory effect of fibrinogen, which was subsequently confirmed in experiments where fibrinogen was removed from plasma or it was added to serum. Finally, gel filtration followed by HIC and MasSpec analysis allow us to identify several possible candidates that were further tested in in vitro experiments. These studies revealed that vitronectin (VTN) is a main factor responsible for observed chemotactic response and that its effect can be inhibited by fibrinogen. Conclusions. Our data indicate that VTN present in normal plasma is a main migration inducing factor responsible for metastasis of malignant cells and that VTN is more potent chemoattractant than already known pro-metastatic chemokines or growth factors. Pro-migratory effect of VTN is neutralized/chaperoned by fibrinogen what may explain preference in migration of tumor cells into lymphatic vessels and body cavities, where concentration of fibrinogen is low. Citation Format: Gabriela Schneider, Nichola C. Garbett, Ewa Bryndza, Agata Poniewierska-Baran, Michael L. Merchant, Karol Serwin, Zachariah P. Sellers, Barbara Dolegowska, Mariusz Z. Ratajczak. Novel evidence that blood plasma vitronectin is a major chemoattractant for cancer cells and its pro-migratory activity is suppressed/chaperoned after binding to fibrinogen. [abstract]. In: Proceedings of the 107th Annual Meeting of the American Association for Cancer Research; 2016 Apr 16-20; New Orleans, LA. Philadelphia (PA): AACR; Cancer Res 2016;76(14 Suppl):Abstract nr 1698.
Gabriela Schneider; Nichola C. Garbett; Ewa Bryndza; Agata Poniewierska-Baran; Michael L. Merchant; Karol Serwin; Zachariah P. Sellers; Barbara Dolegowska; Mariusz Z. Ratajczak. Abstract 1698: Novel evidence that blood plasma vitronectin is a major chemoattractant for cancer cells and its pro-migratory activity is suppressed/chaperoned after binding to fibrinogen. Tumor Biology 2016, 76, 1698 -1698.
AMA StyleGabriela Schneider, Nichola C. Garbett, Ewa Bryndza, Agata Poniewierska-Baran, Michael L. Merchant, Karol Serwin, Zachariah P. Sellers, Barbara Dolegowska, Mariusz Z. Ratajczak. Abstract 1698: Novel evidence that blood plasma vitronectin is a major chemoattractant for cancer cells and its pro-migratory activity is suppressed/chaperoned after binding to fibrinogen. Tumor Biology. 2016; 76 ():1698-1698.
Chicago/Turabian StyleGabriela Schneider; Nichola C. Garbett; Ewa Bryndza; Agata Poniewierska-Baran; Michael L. Merchant; Karol Serwin; Zachariah P. Sellers; Barbara Dolegowska; Mariusz Z. Ratajczak. 2016. "Abstract 1698: Novel evidence that blood plasma vitronectin is a major chemoattractant for cancer cells and its pro-migratory activity is suppressed/chaperoned after binding to fibrinogen." Tumor Biology 76, no. : 1698-1698.
Wojciech Blogowski; Daria Salata; Marta Budkowska; Ewa Zuba-Surma; Karol Serwin; Barbara Dolegowska; Marek Lokaj; Piotr Prowans; Teresa Starzynska. A Translational Attempt to Understand Selected Aspects of ,,Bone-Fat Axis” Function in Humans. Medicine & Science in Sports & Exercise 2014, 46, 636 .
AMA StyleWojciech Blogowski, Daria Salata, Marta Budkowska, Ewa Zuba-Surma, Karol Serwin, Barbara Dolegowska, Marek Lokaj, Piotr Prowans, Teresa Starzynska. A Translational Attempt to Understand Selected Aspects of ,,Bone-Fat Axis” Function in Humans. Medicine & Science in Sports & Exercise. 2014; 46 ():636.
Chicago/Turabian StyleWojciech Blogowski; Daria Salata; Marta Budkowska; Ewa Zuba-Surma; Karol Serwin; Barbara Dolegowska; Marek Lokaj; Piotr Prowans; Teresa Starzynska. 2014. "A Translational Attempt to Understand Selected Aspects of ,,Bone-Fat Axis” Function in Humans." Medicine & Science in Sports & Exercise 46, no. : 636.
It has been suggested that action of complement cascade [CC]-derived anaphylatoxins/molecules may represent a missing link between obesity and metabolic disorders. However, to date, the direct biochemical/immunomodulatory composition of the human AT environment remains poorly understood. In this study, we examined plasma and AT (subcutaneous and visceral/omental) levels of selected CC-derived anaphylatoxins/molecules, and adipsin as well as verified their associations with immune and stem cells chemoattractant - stromal-derived factor-1 (SDF-1).
Wojciech Błogowski; Marta Budkowska; Daria Sałata; Karol Serwin; Barbara Dołęgowska; Marek Łokaj; Piotr Prowans; Teresa Starzyńska. Clinical analysis of selected complement-derived molecules in human adipose tissue. Journal of Translational Medicine 2013, 11, 11 -11.
AMA StyleWojciech Błogowski, Marta Budkowska, Daria Sałata, Karol Serwin, Barbara Dołęgowska, Marek Łokaj, Piotr Prowans, Teresa Starzyńska. Clinical analysis of selected complement-derived molecules in human adipose tissue. Journal of Translational Medicine. 2013; 11 (1):11-11.
Chicago/Turabian StyleWojciech Błogowski; Marta Budkowska; Daria Sałata; Karol Serwin; Barbara Dołęgowska; Marek Łokaj; Piotr Prowans; Teresa Starzyńska. 2013. "Clinical analysis of selected complement-derived molecules in human adipose tissue." Journal of Translational Medicine 11, no. 1: 11-11.
The α-chemokine CXCL12 (stromal derived factor-1; SDF-1) and its corresponding G(αI) protein-coupled CXCR4 receptor axis play an important role in retention of hematopoietic stem progenitor cells (HSPCs) in bone marrow (BM) stem cell niches. CXCL12 has also been identified as a strong chemoattractant for HSPCs and implicated both in homing of HSPCs to BM after transplantation and in egress of these cells from BM into peripheral blood (PB). However, since CXCL12, as a peptide, is highly susceptible to degradation by proteolytic enzymes, its real biological availability in biological fluids may be somewhat limited. In this review, we will present data demonstrating that the CXCL12-CXCR4 axis is positively modulated by innate immunity-derived several external factors, ensuring that even low (near threshold) doses of CXCL12 still exert a robust chemotactic influence on HSPCs.
Mariusz Z. Ratajczak; Karol Serwin; Gabriela Schneider. Innate Immunity Derived Factors as External Modulators of the CXCL12 - CXCR4 Axis and Their Role in Stem Cell Homing and Mobilization. Theranostics 2013, 3, 3 -10.
AMA StyleMariusz Z. Ratajczak, Karol Serwin, Gabriela Schneider. Innate Immunity Derived Factors as External Modulators of the CXCL12 - CXCR4 Axis and Their Role in Stem Cell Homing and Mobilization. Theranostics. 2013; 3 (1):3-10.
Chicago/Turabian StyleMariusz Z. Ratajczak; Karol Serwin; Gabriela Schneider. 2013. "Innate Immunity Derived Factors as External Modulators of the CXCL12 - CXCR4 Axis and Their Role in Stem Cell Homing and Mobilization." Theranostics 3, no. 1: 3-10.
W Blogowski; Karol Serwin; M Budkowska; D Salata; B Dolegowska; M Lokaj; Piotr Prowans; Teresa Starzyńska. Clinical analysis of systemic and adipose tissue levels of selected hormones/adipokines and stromal-derived factor-1. Journal of biological regulators and homeostatic agents 2012, 26, 1 .
AMA StyleW Blogowski, Karol Serwin, M Budkowska, D Salata, B Dolegowska, M Lokaj, Piotr Prowans, Teresa Starzyńska. Clinical analysis of systemic and adipose tissue levels of selected hormones/adipokines and stromal-derived factor-1. Journal of biological regulators and homeostatic agents. 2012; 26 (4):1.
Chicago/Turabian StyleW Blogowski; Karol Serwin; M Budkowska; D Salata; B Dolegowska; M Lokaj; Piotr Prowans; Teresa Starzyńska. 2012. "Clinical analysis of systemic and adipose tissue levels of selected hormones/adipokines and stromal-derived factor-1." Journal of biological regulators and homeostatic agents 26, no. 4: 1.
3431 Background. The greatest problem in cancer therapy is the tendency of cancerous cells to leave a primary tumor and metastasize to different vital organs. Several candidate classes of molecules have been proposed to direct the metastatic process, including chemokines, growth factors, cytokines and bioactive lipids. It is well known that plasma and serum alone possess chemotactic activity, but all the factors responsible for this effect have not been well characterized. This activity was usually assigned to chemokines (e.g., stromal derived factor-1, SDF-1) and growth factors (e.g., hepatocyte growth factor/scatter factor, HGF/SF) present in the serum. However, the concentration of these factors, as measured by ELISA, is negligibly low and does not explain the robust chemotactic responsiveness of tumor cells to serum samples. To our surprise, however, we found that highly diluted (1%) plasma possesses remarkable chemokinetic activity against cancer cell lines, which exceeds by several times the activity of optimal doses of SDF-1 or HGF/SF. Aim of the study. Based on this intriguing observation, our aim was to better characterize this remarkable chemokinetic activity of normal plasma identified in plasma diluted to 1%. Experimental strategies. We employed several human cell lines (lung cancer A549 and HTB177, breast cancer HTB26, cervical carcinoma HTB35, and rhabdomyosracomas RH30 and SMS-CTR) and different dilutions of normal human, murine, and bovine plasma and serum in Transwell migration, adhesion, and cell signaling studies. In chemotactic experiments we also employed samples of human interstitial, pleural, and peritoneal cavity fluids. We tested the effect of heat inactivation, protease exposure, dialysis, and molecular filtration on the chemotactic activity of diluted plasma. Results. We report for the first time that highly diluted (1%) plasma (human, murine, and bovine) possesses remarkable chemotactic activity against malignant tumor cells in Transwell migration assays. This activity rapidly decreases with increasing concentrations of plasma (>5%). We found that in our cell lines, plasma diluted 1:100 activated phosphorylation of MAPKp42/44 and AKT, and the chemotactic response was inhibited by blocking Gai protein signaling by pertussis toxin (PTX) as well as by inhibition of MAPKp42/44 and AKT, which suggests the involvement of a specific GaI protein-coupled receptor. Our initial characterization of this activity revealed that this remarkable factor is sensitive to proteolytic treatment, is not removed from plasma by dialysis, and is temperature-sensitive, which collectively indicates a polypeptide structure. Electrophoretic studies revealed that this factor possesses a molecular weight between 100 and 50 kD. A similar effect has been observed with serum diluted 1:100, though in contrast with plasma, this chemotactic responsiveness was maintained at higher concentrations. This difference between plasma and serum in...
Gabriela Schneider; Karol Serwin; Ewa Bryndza; Magdalena Kucia; Janina Ratajczak; Mariusz Z. Ratajczak. Studies with Diluted Plasma Reveal the Presence of a Remarkably Potent Factor That Enhances the Motility of Cancer Cells and Is Quentched by Fibrinogen - a Novel View of Cancer Metastasis. Blood 2012, 120, 3431 -3431.
AMA StyleGabriela Schneider, Karol Serwin, Ewa Bryndza, Magdalena Kucia, Janina Ratajczak, Mariusz Z. Ratajczak. Studies with Diluted Plasma Reveal the Presence of a Remarkably Potent Factor That Enhances the Motility of Cancer Cells and Is Quentched by Fibrinogen - a Novel View of Cancer Metastasis. Blood. 2012; 120 (21):3431-3431.
Chicago/Turabian StyleGabriela Schneider; Karol Serwin; Ewa Bryndza; Magdalena Kucia; Janina Ratajczak; Mariusz Z. Ratajczak. 2012. "Studies with Diluted Plasma Reveal the Presence of a Remarkably Potent Factor That Enhances the Motility of Cancer Cells and Is Quentched by Fibrinogen - a Novel View of Cancer Metastasis." Blood 120, no. 21: 3431-3431.
Recent studies have shown that adipose tissue (AT), while implicated in orchestrating the sophisticated process termed “immunometabolism,” may also serve as a potential niche for various bone marrow-derived (stem) cells. However, at present, the direct biochemical and immunomodulatory composition of the human AT environment has not been studied. Several substances that might play a crucial role in regulating stem cell migration and/or homing to AT, have been implicated, namely, hepatocyte/vascular endothelial growth factor (VEGF/HGF), leukemia inhibitory factor (LIF), and sphingosine-1-phosphate (SIP). Therefore, we examined and compared the AT concentrations of these substances between plasma, subcutaneous, and omental AT samples derived from 35 generally healthy subjects. VEGF, HGF, LIF, and metalloproteinases (MMP)-2 and MMP9 levels were measured using ELISA, and S1P concentrations were analyzed using reverse-phase high performance liquid chromatography. We found that AT levels of analyzed growth/inhibitory factors were generally comparable (VEGF and LIF) or even higher (HGF) than the corresponding levels in the peripheral blood, particularly in overweight/obese subjects. In subcutaneous AT, significantly lower VEGF and LIF concentrations were observed, and these were accompanied by higher MMP levels. No depot-specific differences in S1P concentrations were found in all examined groups. Moreover, we established several associations between analyzed molecular substances and body composition, BMI, or adiposity index of the examined patients. In conclusion, our study revealed that human AT possesses relatively high levels of selected growth/inhibitory factors and of chemoattractants involved in the regulation of stem cell trafficking, and these factors are associated with the metabolic status of an individual. Further studies are needed to clearly establish the role of these factors in the regulation of bone marrow-derived (stem) cell homeostasis and homing in human AT.
W. Błogowski; Karol Serwin; D. Sałata; Marta Budkowska; B. Dołęgowska; M. Łokaj; Piotr Prowans; Teresa Starzyńska. Plasma and Adipose Tissue Levels of Selected Growth/Inhibitory Factors, Proteolytic Enzymes and Sphingosine-1-Phosphate in Humans. European Journal of Inflammation 2012, 10, 279 -288.
AMA StyleW. Błogowski, Karol Serwin, D. Sałata, Marta Budkowska, B. Dołęgowska, M. Łokaj, Piotr Prowans, Teresa Starzyńska. Plasma and Adipose Tissue Levels of Selected Growth/Inhibitory Factors, Proteolytic Enzymes and Sphingosine-1-Phosphate in Humans. European Journal of Inflammation. 2012; 10 (3):279-288.
Chicago/Turabian StyleW. Błogowski; Karol Serwin; D. Sałata; Marta Budkowska; B. Dołęgowska; M. Łokaj; Piotr Prowans; Teresa Starzyńska. 2012. "Plasma and Adipose Tissue Levels of Selected Growth/Inhibitory Factors, Proteolytic Enzymes and Sphingosine-1-Phosphate in Humans." European Journal of Inflammation 10, no. 3: 279-288.