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Dr. Liangxiao Zhang
Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan, China

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0 Analytical Chemistry
0 Chemometrics
0 Gas Chromatography
0 Mass Spectrometry
0 NIR Spectroscopy

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Mass Spectrometry
Gas Chromatography
LC-MS/MS
Chemometrics
Analytical Chemistry
NIR Spectroscopy

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Journal article
Published: 31 August 2021 in Metabolites
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The simultaneous quantification of trace and micro metabolites is a bottleneck in food and biological analysis. Phenolic compounds are the most widely distributed and have various physiological functions. In this study, the strategy for the simultaneous liquid chromatography tandem-mass spectrometry (LC-MS/MS) quantification of 13 trace and micro phenolic compounds was proposed by taking product ions and isotopic ions as quantitative ions. The method validation results showed that the limits of detection (LODs) were from 0.01 to 9.84 μg/kg, and the limits of quantification (LOQs) were from 0.03 to 32.8 μg/kg. The intra-day precision and inter-day precision were below 8.4% and 14.4%, respectively. The recoveries ranged from 81.9% to 117.2%, and the matrix effects ranged from −11.5% to 13.7%, which indicated that the method has high sensitivity and suitable stability. The developed analytical method was applied to determine trace and micro constituents in rapeseed samples. The analysis results indicated that the contents of sinapine have significantly different between high and low total phenolic content rapeseeds. This method provides a reference strategy for the simultaneous quantitative analysis of other micro- and trace antioxidants.

ACS Style

Dandan Wang; Liangxiao Zhang; Li Yu; Fei Ma; Peiwu Li. Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry. Metabolites 2021, 11, 589 .

AMA Style

Dandan Wang, Liangxiao Zhang, Li Yu, Fei Ma, Peiwu Li. Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry. Metabolites. 2021; 11 (9):589.

Chicago/Turabian Style

Dandan Wang; Liangxiao Zhang; Li Yu; Fei Ma; Peiwu Li. 2021. "Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry." Metabolites 11, no. 9: 589.

Paper
Published: 01 June 2021 in RSC Advances
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An ultrasensitive DNA biosensor based on the fluorescence quenching ability of FOQDs towards FAM–ssDNA through π–π stacking interactions between ssDNA and FOQDs.

ACS Style

Hui Li; Hua Pang; Liangxiao Zhang; Jin Mao; Wen Zhang; Jun Jiang; Peiwu Li; Qi Zhang. Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots. RSC Advances 2021, 11, 19690 -19694.

AMA Style

Hui Li, Hua Pang, Liangxiao Zhang, Jin Mao, Wen Zhang, Jun Jiang, Peiwu Li, Qi Zhang. Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots. RSC Advances. 2021; 11 (32):19690-19694.

Chicago/Turabian Style

Hui Li; Hua Pang; Liangxiao Zhang; Jin Mao; Wen Zhang; Jun Jiang; Peiwu Li; Qi Zhang. 2021. "Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots." RSC Advances 11, no. 32: 19690-19694.

Journal article
Published: 09 July 2020 in Oil Crop Science
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To ensure authenticity of sesame oil, an authentication technology was proposed using ion mobility spectrometry (IMS) and chemometrics. One-class classification (OCC) methods including one-class partial least squares (OCPLS) and one-class support vector machine (OCSVM) were employed to build authentication models for sesame oil. Subsequently, an independent test set was used to validate the constructed models. Validation set of 45 adulterated oils indicated that the prediction correction rate of OCPLS model reached 95.6% (43 out of 45). Moreover, the complete set of sesame oils adulterated by sesame oil essence could be identified as counterfeit. Compared with previous studies, OCPLS model could work to identify untargeted adulteration. In conclusion, OCC method could effectively detect adulterated sesame oils containing as little as 10% of other vegetable oils. This study provided a rapid screening method for adulterated sesame oil in market surveillance and a reference for developing authentication methods of other edible oils.

ACS Style

Jun Jiang; Xinjing Dou; Liangxiao Zhang; Jin Mao; Li Yu; Fei Ma; Peiwu Li. Rapid authentication of sesame oil using ion mobility spectrometry and chemometrics. Oil Crop Science 2020, 5, 161 -165.

AMA Style

Jun Jiang, Xinjing Dou, Liangxiao Zhang, Jin Mao, Li Yu, Fei Ma, Peiwu Li. Rapid authentication of sesame oil using ion mobility spectrometry and chemometrics. Oil Crop Science. 2020; 5 (4):161-165.

Chicago/Turabian Style

Jun Jiang; Xinjing Dou; Liangxiao Zhang; Jin Mao; Li Yu; Fei Ma; Peiwu Li. 2020. "Rapid authentication of sesame oil using ion mobility spectrometry and chemometrics." Oil Crop Science 5, no. 4: 161-165.

Review
Published: 12 May 2020 in Trends in Food Science & Technology
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Edible oils play a vital role in our daily life, which provide human beings with energy, essential fatty acids and nutrients. The quality of edible oils can be dependent on the quality of the oilseeds they originate from, and their adulteration during processing. In recent years, near infrared spectroscopy was widely used in the rapid assessment of quality of oilseeds and edible oils. However, to the best of our knowledge, a comprehensive review on near infrared spectroscopy for the quality of oilseeds remains to be published. The applications of near infrared spectroscopy in the quality of oilseeds and edible oils have been emphasized in this review. This article briefly summarizes the basic knowledge of near infrared spectroscopy. In addition, we highlight the application of this technique on the detection of physicochemical properties and quality, specific nutrient components, authentication, and geographical origin traceability of oilseeds and edible oils. Moreover, the application of near infrared hyperspectral imaging technology in oilseeds has been addressed. Key findings and conclusions: Near infrared spectroscopy possesses the advantages of rapid, green and low-cost analysis. Meanwhile, it is also a nondestructive method and therefore suitable to quality analysis of oilseeds in agricultural sciences. It can be used to detect macro nutrients in oilseeds and edible oils by combining NIR and advanced chemometrics. In the future, NIR could be applied in the rapid detection and online detection of hazardous substances and nutrients in oilseeds by developing the new instrument and chemometric methods.

ACS Style

Xue Li; Liangxiao Zhang; Yong Zhang; Du Wang; Xuefang Wang; Li Yu; Wen Zhang; Peiwu Li. Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils. Trends in Food Science & Technology 2020, 101, 172 -181.

AMA Style

Xue Li, Liangxiao Zhang, Yong Zhang, Du Wang, Xuefang Wang, Li Yu, Wen Zhang, Peiwu Li. Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils. Trends in Food Science & Technology. 2020; 101 ():172-181.

Chicago/Turabian Style

Xue Li; Liangxiao Zhang; Yong Zhang; Du Wang; Xuefang Wang; Li Yu; Wen Zhang; Peiwu Li. 2020. "Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils." Trends in Food Science & Technology 101, no. : 172-181.

Journal article
Published: 04 May 2020 in Molecules
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Rapeseed (Brassica napus L.) is rich in phenols, vitamins, carotenoids, and mineral elements, such as selenium. Additionally, it contains the active ingredients sulforaphane and indole-3-carbinol, which have been demonstrated to have pharmacological effects. In this study, sulforaphane and indole-3-carbinol were extracted and quantified from rapeseeds using quick, easy, cheap, effective, rugged and safe (QuEChERS) method coupled with ultra high performance liquid chromarography tandem mass spectrometry (UHPLC-MS/MS). The major parameters for extraction and purification efficiency were optimized, including the hydrolysis reaction, extraction condition and type and amount of purification adsorbents. The limit of detection (LOD) and the limit of quantification (LOQ) for sulforaphane were 0.05 μg/kg and 0.15 μg/kg, and for indole-3-carbinol were 5 μg/kg and 15 μg/kg, respectively. The developed method was used to successfully analyze fifty rapeseed samples. The QuEChERS coupled with UHPLC-MS/MS simultaneously detect sulforaphane and indole-3-carbinol in vegetable matrix and evaluate the quality and nutrition of rapeseed samples.

ACS Style

Xu Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS. Molecules 2020, 25, 2149 .

AMA Style

Xu Yu, Fei Ma, Liangxiao Zhang, Peiwu Li. Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS. Molecules. 2020; 25 (9):2149.

Chicago/Turabian Style

Xu Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. 2020. "Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS." Molecules 25, no. 9: 2149.

Article
Published: 04 April 2020 in Food Analytical Methods
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Volatile flavor components are critical to edible oils and influence consumption choice. Meanwhile, volatile organic compounds (VOCs) in edible oils are also potential biomarkers to detect adulterated products. However, VOCs are usually complex, unstable, and easily influenced by oil processing and even analysis conditions. Here, we report headspace solid-phase microextraction for comprehensive two-dimensional gas chromatography mass spectrometric analysis of VOCs in edible oils as being optimized and validated using central composite design (CCD). The results indicated that the optimal extraction conditions are as follows: SPE column of divinylbenzene/carboxen/polydimethylsiloxane (DVB/CAR/PDMS), equilibration time of 10.3 min, and extraction time of 19.6 min at 120 °C. This method could analyze edible oils to identify characteristic flavor components. As a result, 25 VOC tags were identified and used to characterize soybean oils. Chemometric methods were used to discriminate the authentic sesame oils from those adulterated with soybean oil. The results indicated that this method is an effective approach to measure VOCs in edible oils; such measurements can report counterfeit products.

ACS Style

Yu Sun; Xinjing Dou; Xiaofeng Yue; Li Yu; Liangxiao Zhang; Ji Li; Peiwu Li. Optimization of Headspace SPME GC × GC-TOF/MS Analysis of Volatile Organic Compounds in Edible Oils by Central Composite Design for Adulteration Detection of Edible Oil. Food Analytical Methods 2020, 13, 1328 -1336.

AMA Style

Yu Sun, Xinjing Dou, Xiaofeng Yue, Li Yu, Liangxiao Zhang, Ji Li, Peiwu Li. Optimization of Headspace SPME GC × GC-TOF/MS Analysis of Volatile Organic Compounds in Edible Oils by Central Composite Design for Adulteration Detection of Edible Oil. Food Analytical Methods. 2020; 13 (6):1328-1336.

Chicago/Turabian Style

Yu Sun; Xinjing Dou; Xiaofeng Yue; Li Yu; Liangxiao Zhang; Ji Li; Peiwu Li. 2020. "Optimization of Headspace SPME GC × GC-TOF/MS Analysis of Volatile Organic Compounds in Edible Oils by Central Composite Design for Adulteration Detection of Edible Oil." Food Analytical Methods 13, no. 6: 1328-1336.

Journal article
Published: 29 February 2020 in Metabolites
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Food adulteration is a challenge faced by consumers and researchers. Due to DNA fragmentation during oil processing, it is necessary to discover metabolic markers alternative to DNA for adulteration detection of edible oils. However, the contents of metabolic markers vary in response to various factors, such as plant species, varieties, geographical origin, climate, and cultivation measures. Thus, it is difficult to identify a universal marker for all adulterants that may be present in some authentic samples. Currently, the specificity and selectivity of metabolic biomarkers are difficult to validate. Therefore, this study developed a screening strategy based on plant metabolic networks by developing a targeted analytical method for 56 metabolites in a metabolic network, using liquid/liquid extraction–liquid chromatography–tandem mass spectrometry (LC-MS/MS). We identified a chain of 11 metabolites that were related to isoflavonoid biosynthesis, which were detected in soybean oils but not rapeseed oils. Through multiple-marker mutual validation, these metabolites can be used as species-specific universal markers to differentiate soybean oil from rapeseed oil. Moreover, this method provides a model for screening characteristic markers of other edible vegetable oils and foods.

ACS Style

Xinjing Dou; Liangxiao Zhang; Xiao Wang; Ruinan Yang; Xuefang Wang; Fei Ma; Li Yu; Jin Mao; Hui Li; Xiupin Wang; Peiwu Li. Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks. Metabolites 2020, 10, 85 .

AMA Style

Xinjing Dou, Liangxiao Zhang, Xiao Wang, Ruinan Yang, Xuefang Wang, Fei Ma, Li Yu, Jin Mao, Hui Li, Xiupin Wang, Peiwu Li. Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks. Metabolites. 2020; 10 (3):85.

Chicago/Turabian Style

Xinjing Dou; Liangxiao Zhang; Xiao Wang; Ruinan Yang; Xuefang Wang; Fei Ma; Li Yu; Jin Mao; Hui Li; Xiupin Wang; Peiwu Li. 2020. "Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks." Metabolites 10, no. 3: 85.

Journal article
Published: 14 February 2020 in Molecules
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Vitamin K1 is one of the important hydrophobic vitamins in fat-containing foods. Traditionally, lipase is employed in the determination of vitamin K1 to remove the lipids, which makes the detection complex, time-consuming, and insensitive. In this study, the determination of vitamin K1 in fat-containing foods was developed based on ultrasound-assisted extraction (UAE), solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS). The optimal conditions for extraction of vitamin K1 were material–liquid ratio of 1:70 (g/mL), extraction temperature of 50 °C, extraction power of 700 W, extraction time of 50 min, material-wash fluid ratio of 1:60 (g/mL), and 8 mL of hexane/anhydrous ether (97:3, v/v) as the elution solvent. Then, vitamin K1 was analyzed on a ZORBAX SB-C18 column (50 mm × 2.1 mm, 1.8 μm) by gradient elution with water (0.01% formic acid) and methanol (0.01 formic acid + 2.5 mmol/L ammonium formate) as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.16 μg/kg, respectively. Calibration curve was linear over the range of 10–500 ng/mL (R2 > 0.9988). The recoveries at three spiked levels were between 80.9% and 119.1%. The validation and application indicated that the proposed method was simple and sensitive in determination of vitamin K1 in fat-containing foods.

ACS Style

Yueqing Xu; Liangxiao Zhang; Ruinan Yang; Xu Yu; Li Yu; Fei Ma; Hui Li; Xiupin Wang; Peiwu Li. Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS. Molecules 2020, 25, 839 .

AMA Style

Yueqing Xu, Liangxiao Zhang, Ruinan Yang, Xu Yu, Li Yu, Fei Ma, Hui Li, Xiupin Wang, Peiwu Li. Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS. Molecules. 2020; 25 (4):839.

Chicago/Turabian Style

Yueqing Xu; Liangxiao Zhang; Ruinan Yang; Xu Yu; Li Yu; Fei Ma; Hui Li; Xiupin Wang; Peiwu Li. 2020. "Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS." Molecules 25, no. 4: 839.

Journal article
Published: 26 October 2019 in Toxins
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In this study, magnetic graphene nanocomposite Fe3O4/rGO was synthesized by facile one-pot solvothermal method. The nanocomposite was successfully used as magnetic solid phase extraction (MSPE) adsorbents for the determination of aflatoxins in edible vegetable oils through the π-π stacking interactions. MSPE parameters including the amount of adsorbents, extraction and desorption time, washing conditions, and the type and volume of desorption solvent were optimized. Under optimal conditions, good linear relationships were achieved. Limits of detection of this method were as low as 0.02 µg/kg and 0.01 µg/kg for aflatoxin B1 and B2, respectively. Finally, the magnetic graphene nanocomposite was successfully applied to aflatoxin analysis in vegetable oils. The results indicated that the recoveries of the B-group aflatoxins ranged from 80.4% to 106.0%, whereas the relative standard deviations (RSDs) were less than 8.1%. Owing to the simplicity, rapidity and efficiency, Fe3O4/rGO magnetic solid phase extraction coupled with high-performance liquid chromatography fluorescence with post-column photochemical derivatization (Fe3O4/rGO MSPE-HPLC-PCD-FLD) is a promising analytical method for routine and accurate determination of aflatoxins in lipid matrices.

ACS Style

Li Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. Determination of Aflatoxin B1 and B2 in Vegetable Oils Using Fe3O4/rGO Magnetic Solid Phase Extraction Coupled with High-Performance Liquid Chromatography Fluorescence with Post-Column Photochemical Derivatization. Toxins 2019, 11, 621 .

AMA Style

Li Yu, Fei Ma, Liangxiao Zhang, Peiwu Li. Determination of Aflatoxin B1 and B2 in Vegetable Oils Using Fe3O4/rGO Magnetic Solid Phase Extraction Coupled with High-Performance Liquid Chromatography Fluorescence with Post-Column Photochemical Derivatization. Toxins. 2019; 11 (11):621.

Chicago/Turabian Style

Li Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. 2019. "Determination of Aflatoxin B1 and B2 in Vegetable Oils Using Fe3O4/rGO Magnetic Solid Phase Extraction Coupled with High-Performance Liquid Chromatography Fluorescence with Post-Column Photochemical Derivatization." Toxins 11, no. 11: 621.

Journal article
Published: 19 August 2019 in Antioxidants
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Sesame is a nutritional agricultural product with medicinal properties. Accurate determination of micronutrients is important for the improvement of sesame quality and nutrition assessments. Our previous study showed that 10 antioxidants—d-homoproline, vitamin B2, coniferyl aldehyde, hesperidin, phloretin, N-acetyl-l-leucine, l-hyoscyamine, ferulic acid, 5-methoxypsoralen, and 8-methoxypsoralen—in sesame were potential characteristic nutrients in sesame. Herein, simultaneous detection of 10 different types of antioxidants was developed by using ultrasound-assisted extraction coupled with liquid chromatography-tandem mass spectrometry (UAE-LC-MS/MS) with the help of response surface methodology. The significant variables and levels were screened and optimized by combining the single factor experiment, Plackett–Burman test, and Box–Behnken design. The optimal conditions for extraction of target antioxidants in sesame were methanol solution of 75.0%, liquid-to-material ratio of 20:1 (mL/g), extraction temperature of 50 °C, extraction power of 410.0 W, extraction time of 65 min. The total yield of targets was 21.74 μg/g under the optimized conditions. The mobile phase used was 0.1% formic acid in acetonitrile and 0.1% formic acid in water, and the column was a Thermo Syncronis C18 reverse phase column (100 mm × 2.1 mm, 3 μm). All targets required only one injection and could be quickly separated and assayed within 7 min. The limits of detection and limits of quantification for these 10 nutritional compounds ranged from 0.01 to 0.11 µg/kg and from 0.04 to 0.34 µg/kg, respectively. The validation results indicated that the method had reasonable linearity (R2 ≥ 0.9990), good recoveries (71.1%–118.3%), satisfactory intra-day precision (≤9.6%) and inter-day precision (≤12.9%), and negligible matrix effects (≤13.8%). This simultaneous quantification method was accurate, fast, and robust for the assessment of sesame nutrition.

ACS Style

Dandan Wang; Liangxiao Zhang; Yueqing Xu; Xin Qi; Xuefang Wang; Xiupin Wang; Qi Zhang; Peiwu Li. Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology. Antioxidants 2019, 8, 321 .

AMA Style

Dandan Wang, Liangxiao Zhang, Yueqing Xu, Xin Qi, Xuefang Wang, Xiupin Wang, Qi Zhang, Peiwu Li. Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology. Antioxidants. 2019; 8 (8):321.

Chicago/Turabian Style

Dandan Wang; Liangxiao Zhang; Yueqing Xu; Xin Qi; Xuefang Wang; Xiupin Wang; Qi Zhang; Peiwu Li. 2019. "Optimization of an Ultrasound-Assisted Extraction for Simultaneous Determination of Antioxidants in Sesame with Response Surface Methodology." Antioxidants 8, no. 8: 321.

Journal article
Published: 09 August 2019 in Foods
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Phytosterols are important micronutrients in human diets. Evidence has shown that phytosterols play an essential role in the reduction of cholesterol in blood and therefore decrease cardiovascular morbidity. In this study, the content and composition of phytosterols in different kinds of vegetable oils were analyzed, and the total phytosterol intake and contribution of foods to intake were estimated based on consumption data. The results showed that the phytosterol contents of rice bran oil, corn oil, and rapeseed oil were higher than those of other vegetable oils and the intake of phytosterol in the Chinese diet was about 392.3 mg/day. The main sources of phytosterols were edible vegetable oils (46.3%), followed by cereals (38.9%), vegetables (9.2%), nuts (2.0%), fruits (1.5%), beans and bean products (1.4%), and tubers (0.8%). Among all vegetable oils, rapeseed oil was the main individual contributor to phytosterol intake (22.9%), especially for the southern residents of China.

ACS Style

Ruinan Yang; Li Xue; Liangxiao Zhang; Xuefang Wang; Xin Qi; Jun Jiang; Li Yu; Xiupin Wang; Wen Zhang; Qi Zhang; Peiwu Li. Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet. Foods 2019, 8, 334 .

AMA Style

Ruinan Yang, Li Xue, Liangxiao Zhang, Xuefang Wang, Xin Qi, Jun Jiang, Li Yu, Xiupin Wang, Wen Zhang, Qi Zhang, Peiwu Li. Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet. Foods. 2019; 8 (8):334.

Chicago/Turabian Style

Ruinan Yang; Li Xue; Liangxiao Zhang; Xuefang Wang; Xin Qi; Jun Jiang; Li Yu; Xiupin Wang; Wen Zhang; Qi Zhang; Peiwu Li. 2019. "Phytosterol Contents of Edible Oils and Their Contributions to Estimated Phytosterol Intake in the Chinese Diet." Foods 8, no. 8: 334.

Journal article
Published: 01 August 2019 in Metabolites
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Rapeseed is an important oilseed with proper fatty acid composition and abundant bioactive components. Canada and China are the two major rapeseed-producing countries all over the world. Meanwhile, Canada and Mongolia are major importers of rapeseed due to the great demand for rapeseed in China. To investigate the metabolites in rapeseeds from three countries, ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF/MS)-based metabolomics was employed to analyze rapeseeds from China, Canada, and Mongolia. As results, 67, 53, and 68 metabolites showed significant differences between Chinese and Canadian, Chinese and Mongolian, and Canadian and Mongolian rapeseeds, respectively. Differential metabolites were mainly distributed in the metabolic pathways including phenylpropanoid biosynthesis, flavone and flavonol biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis. Among the differential metabolites, contents of sinapate and sinapine were higher in Chinese rapeseeds, while the contents of brassicasterol, stigmasterol, and campestanol were higher in Canadian rapeseeds. These findings might provide insight into the metabolic characteristics of rapeseeds from three countries to guide processing and consumption of the products of rapeseed.

ACS Style

Ruinan Yang; Ligang Deng; Liangxiao Zhang; Xiaofeng Yue; Jin Mao; Fei Ma; Xiupin Wang; Qi Zhang; Wen Zhang; Peiwu Li. Comparative Metabolomic Analysis of Rapeseeds from Three Countries. Metabolites 2019, 9, 161 .

AMA Style

Ruinan Yang, Ligang Deng, Liangxiao Zhang, Xiaofeng Yue, Jin Mao, Fei Ma, Xiupin Wang, Qi Zhang, Wen Zhang, Peiwu Li. Comparative Metabolomic Analysis of Rapeseeds from Three Countries. Metabolites. 2019; 9 (8):161.

Chicago/Turabian Style

Ruinan Yang; Ligang Deng; Liangxiao Zhang; Xiaofeng Yue; Jin Mao; Fei Ma; Xiupin Wang; Qi Zhang; Wen Zhang; Peiwu Li. 2019. "Comparative Metabolomic Analysis of Rapeseeds from Three Countries." Metabolites 9, no. 8: 161.

Journal article
Published: 08 March 2019 in LWT
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Simultaneous determination of 19 phenolic compounds in oilseeds was performed using liquid chromatography tandem mass spectrometry (LC-MS/MS) combined with magnetic solid phase extraction (M-SPE). Magnetic graphene (Fe3O4-r-GO) was synthesized via a one-step hydrothermal method. The optimum extraction conditions were 4 mg Fe3O4/r-GO, 2 mL of 5% methanol-water as the dispersing solvent, and 6 mL of 0.1% formic acid-methanol as the elution solvent. The limits of detection and quantification were 0.02–90 μg/kg and 0.06–300 μg/kg, and the recoveries of polyphenols in the oilseeds ranged from 71.2% to 116.6%; both the inter-day and intra-day precisions were less than 10.8%. This method is efficient, sensitive, and stable; it can simultaneously measure many phenolic compounds in oilseeds.

ACS Style

Huhu Lang; Ruinan Yang; Xinjing Dou; Dandan Wang; Liangxiao Zhang; Ji Li; Peiwu Li. Simultaneous determination of 19 phenolic compounds in oilseeds using magnetic solid phase extraction and LC-MS/MS. LWT 2019, 107, 221 -227.

AMA Style

Huhu Lang, Ruinan Yang, Xinjing Dou, Dandan Wang, Liangxiao Zhang, Ji Li, Peiwu Li. Simultaneous determination of 19 phenolic compounds in oilseeds using magnetic solid phase extraction and LC-MS/MS. LWT. 2019; 107 ():221-227.

Chicago/Turabian Style

Huhu Lang; Ruinan Yang; Xinjing Dou; Dandan Wang; Liangxiao Zhang; Ji Li; Peiwu Li. 2019. "Simultaneous determination of 19 phenolic compounds in oilseeds using magnetic solid phase extraction and LC-MS/MS." LWT 107, no. : 221-227.

Journal article
Published: 11 February 2019 in Toxins
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Deoxynivalenol (DON) is a secondary metabolite produced by Fusarium, which is a trichothecene mycotoxin. As the main mycotoxin with high toxicity, wheat, barley, corn and their products are susceptible to contamination of DON. Due to the stability of this mycotoxin, traditional methods for DON reduction often require a strong oxidant, high temperature and high pressure with more energy consumption. Therefore, exploring green, efficient and environmentally friendly ways to degrade or reduce DON is a meaningful and challenging issue. Herein, a dendritic-like α-Fe2O3 was successfully prepared using a facile hydrothermal synthesis method at 160 °C, which was systematically characterized by X-ray diffraction (XRD), high-resolution transmission electron microscopy (HRTEM), scanning electron microscopy (SEM), and X-ray photoelectron spectroscopy (XPS). It was found that dendritic-like α-Fe2O3 showed superior activity for the photocatalytic degradation of DON in aqueous solution under visible light irradiation (λ > 420 nm) and 90.3% DON (initial concentration of 4.0 μg/mL) could be reduced in 2 h. Most of all, the main possible intermediate products were proposed through high performance liquid chromatography-mass spectrometry (HPLC-MS) after the photocatalytic treatment. This work not only provides a green and promising way to mitigate mycotoxin contamination but also may present useful information for future studies.

ACS Style

Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins 2019, 11, 105 .

AMA Style

Huiting Wang, Jin Mao, Zhaowei Zhang, Qi Zhang, Liangxiao Zhang, Peiwu Li, Wen Zhang. Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation. Toxins. 2019; 11 (2):105.

Chicago/Turabian Style

Huiting Wang; Jin Mao; Zhaowei Zhang; Qi Zhang; Liangxiao Zhang; Peiwu Li; Wen Zhang. 2019. "Photocatalytic degradation of deoxynivalenol over dendritic-like α-Fe2O3 under visible light irradiation." Toxins 11, no. 2: 105.

Research article
Published: 13 December 2018 in European Journal of Lipid Science and Technology
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The residues of chlorophyll induce oxidative rancidity and deterioration through photo‐oxidation. However, the effect of chlorophyll on the quality of rapeseed oil is still unknown. In this study, chlorophyll was added into rapeseed oil to reach the desired treatment levels of 2 mg/kg, 5 mg/kg and 10 mg/kg, while no chlorophyll was added in the control sample. We investigated dynamic changes of the peroxide value of rapeseed oils with different chlorophyll contents exposed to light and stored in the dark at different times (0, 1, 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, and 60 days). The results showed that the peroxide value of rapeseed oil exposed to light increased significantly, especially for the oil with a high content of chlorophyll. In contrast, the peroxide value of rapeseed oil stored in dark was nearly unchanged no matter whether extra chlorophyll was added. This study showed that chlorophyll in rapeseed oil could trigger oil oxidation, and therefore it is necessary to take measures to reduce the chlorophyll content in rapeseed oil or store rapeseed oil in the dark as soon as possible. Practical applications: This study showed that chlorophyll in rapeseed oil could trigger oil oxidation, and therefore it is necessary to take measures to reduce the chlorophyll content in rapeseed oil while saving oil in the dark.

ACS Style

Xue Li; Ruinan Yang; Chunling Lv; Lin Chen; Liangxiao Zhang; Xiaoxia Ding; Wen Zhang; Qi Zhang; Chundi Hu; Peiwu Li. Effect of Chlorophyll on Lipid Oxidation of Rapeseed Oil. European Journal of Lipid Science and Technology 2018, 121, 1 .

AMA Style

Xue Li, Ruinan Yang, Chunling Lv, Lin Chen, Liangxiao Zhang, Xiaoxia Ding, Wen Zhang, Qi Zhang, Chundi Hu, Peiwu Li. Effect of Chlorophyll on Lipid Oxidation of Rapeseed Oil. European Journal of Lipid Science and Technology. 2018; 121 (4):1.

Chicago/Turabian Style

Xue Li; Ruinan Yang; Chunling Lv; Lin Chen; Liangxiao Zhang; Xiaoxia Ding; Wen Zhang; Qi Zhang; Chundi Hu; Peiwu Li. 2018. "Effect of Chlorophyll on Lipid Oxidation of Rapeseed Oil." European Journal of Lipid Science and Technology 121, no. 4: 1.

Research article
Published: 16 November 2018 in Analytical Chemistry
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Aflatoxins, highly toxic and carcinogenic to humans, are synthesized via multiple intermediates by a complex pathway in several Aspergilli including Aspergillus flavus. Few analytical methods are available for monitoring the changes in metabolite profiles of the aflatoxin biosynthesis pathway under different growth and environmental conditions. In the present study, we developed by D-optimal mixture design a solvent system, methanol/dichloromethane/ethyl acetate/formic acid (0.36/0.31/0.32/0.01), that was suitable for extracting the pathway metabolites. Matrix effect from dilution of cell extracts was negligible. To facilitate the identification of these metabolites, we constructed a fragmentation ion library. We further employed liquid chromatography coupled with high-resolution mass spectroscopy (UHPLC-HRMS) for simultaneous quantification of the metabolites. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.002-0.016 μg/kg and 0.008-0.05 μg/kg, respectively. The spiked recovery rates ranged from 81.3% to 100.3% with intra-day and inter-day precision to be less than 7.6%. Using the method developed to investigate time-course aflatoxin biosynthesis, we found that precursors, including several possible toxins (with a carcinogenic group similar to aflatoxin B1) occurred together with aflatoxin, production increased rapidly at the early growth stage, peaked on day four and then decreased substantially. The maximum production of aflatoxin B1 and aflatoxin B2 occurred one day later. Moreover, the dominant branch pathway was the one for aflatoxin B1 formation. We revealed that the anti-aflatoxigenicity mechanism of Leclercia adecarboxylata WT16 was associated with a factor upstream of the aflatoxin biosynthesis pathway. The design strategies can be applied to characterize or detect other secondary metabolites to provide a snapshot of the dynamic changes during their biosynthesis.

ACS Style

Huali Xie; Xiupin Wang; Liangxiao Zhang; Tong Wang; Wen Zhang; Jun Jiang; Perng-Kuang Chang; Zhi-Yuan Chen; Deepak Bhatnagar; Qi Zhang; Peiwu Li. Monitoring Metabolite Production of Aflatoxin Biosynthesis by Orbitrap Fusion Mass Spectrometry and a D-Optimal Mixture Design Method. Analytical Chemistry 2018, 90, 14331 -14338.

AMA Style

Huali Xie, Xiupin Wang, Liangxiao Zhang, Tong Wang, Wen Zhang, Jun Jiang, Perng-Kuang Chang, Zhi-Yuan Chen, Deepak Bhatnagar, Qi Zhang, Peiwu Li. Monitoring Metabolite Production of Aflatoxin Biosynthesis by Orbitrap Fusion Mass Spectrometry and a D-Optimal Mixture Design Method. Analytical Chemistry. 2018; 90 (24):14331-14338.

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Huali Xie; Xiupin Wang; Liangxiao Zhang; Tong Wang; Wen Zhang; Jun Jiang; Perng-Kuang Chang; Zhi-Yuan Chen; Deepak Bhatnagar; Qi Zhang; Peiwu Li. 2018. "Monitoring Metabolite Production of Aflatoxin Biosynthesis by Orbitrap Fusion Mass Spectrometry and a D-Optimal Mixture Design Method." Analytical Chemistry 90, no. 24: 14331-14338.

Original research
Published: 11 October 2018 in Food Science & Nutrition
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The radical scavenging capabilities of the extracts from eleven edible vegetable oils were investigated by using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), 2,2′‐azino‐bis‐3‐ ethylbenzothiazoline‐6‐sulfonic acid (ABTS), and ferric reducing ability of plasma (FRAP) assays. The results indicated that rapeseed oil and sesame oil showed higher radical scavenging abilities than other vegetable oils. When the radical scavenging capabilities of the extracts from virgin camellia oils and commercially available refined camellia oils were evaluated by FRAP assay, the results showed that the antioxidant capabilities of the former were higher than the latter. Therefore, it is recommended that moderate refining processes should be taken to minimize the loss of antioxidant components and people consume virgin oils or less processed edible vegetable oils for higher antioxidant activities.

ACS Style

Sujun Wang; Ruinan Yang; Hui Li; Jun Jiang; Liangxiao Zhang; Qi Zhang; Peiwu Li. Evaluation and comparison of in vitro antioxidant activities of unsaponifiable fraction of 11 kinds of edible vegetable oils. Food Science & Nutrition 2018, 6, 2355 -2362.

AMA Style

Sujun Wang, Ruinan Yang, Hui Li, Jun Jiang, Liangxiao Zhang, Qi Zhang, Peiwu Li. Evaluation and comparison of in vitro antioxidant activities of unsaponifiable fraction of 11 kinds of edible vegetable oils. Food Science & Nutrition. 2018; 6 (8):2355-2362.

Chicago/Turabian Style

Sujun Wang; Ruinan Yang; Hui Li; Jun Jiang; Liangxiao Zhang; Qi Zhang; Peiwu Li. 2018. "Evaluation and comparison of in vitro antioxidant activities of unsaponifiable fraction of 11 kinds of edible vegetable oils." Food Science & Nutrition 6, no. 8: 2355-2362.

Short communication
Published: 29 July 2018 in Chemometrics and Intelligent Laboratory Systems
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In natural science, models could grant new insights into phenomena or scientific problems which are hard to be observed or otherwise explained to overcome the limitations of human beings. Routinely, scientists strive to develop new methods for data acquisition, preprocessing, variable selection, modeling and valuation with the help of statistics and machine learning theories. Theoretically, the aim of these methods is global or local optimization in the space of variables and linear/nonlinear combinations for classification or regression. However, the relationships between responses and features are often complex and therefore sometimes far from linear or fixed nonlinear model. In this study, we proposed the relational variable (e.g. ratio between two variables) for more accurate prediction performance of models and illustrated its application on three classic data. We found that the selected relational variables could significantly improve the accuracy of prediction. The software was complemented on the MATLAB R2015a platform in Windows Server 2012 R2 standard. The Matlab codes used in this study are publicly available at http://www.libpls.net.

ACS Style

Zhe Yuan; Liangxiao Zhang; Ruinan Yang; Jin Mao; Qi Zhang; Peiwu Li. Relational variable for more accurate prediction of models. Chemometrics and Intelligent Laboratory Systems 2018, 180, 84 -87.

AMA Style

Zhe Yuan, Liangxiao Zhang, Ruinan Yang, Jin Mao, Qi Zhang, Peiwu Li. Relational variable for more accurate prediction of models. Chemometrics and Intelligent Laboratory Systems. 2018; 180 ():84-87.

Chicago/Turabian Style

Zhe Yuan; Liangxiao Zhang; Ruinan Yang; Jin Mao; Qi Zhang; Peiwu Li. 2018. "Relational variable for more accurate prediction of models." Chemometrics and Intelligent Laboratory Systems 180, no. : 84-87.

Journal article
Published: 15 May 2018 in Molecules
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Chemical composition of secondary metabolites is of great importance for quality control of agricultural products. Black sesame seeds are significantly more expensive than white sesame seeds, because it is thought that black sesame seeds are more beneficial to human health than white sesame seeds. However, the differences in nutrient composition between black sesame seeds and white sesame seeds are still unknown. The current study examined the levels of different metabolites in black and white sesame seeds via the use of a novel metabolomics strategy. Using widely targeted metabolomics data, we obtained the structure and content of 557 metabolites, out of which 217 metabolites were identified, and discovered 30 metabolic pathways activated by the secondary metabolites in both black and white sesame seeds. Our results demonstrated that the main pathways that were differentially activated included: phenylpropanoid biosynthesis, tyrosine metabolism, and riboflavin metabolism. More importantly, the biomarkers that were significantly different between black seeds and white sesame seeds are highly related to the functions recorded in traditional Chinese medicine. The results of this study may serve as a new theoretical reference for breeding experts to promote the genetic improvement of sesame seeds, and therefore the cultivation of higher quality sesame varieties.

ACS Style

Dandan Wang; Liangxiao Zhang; Xiaorong Huang; Xiao Wang; Ruinan Yang; Jin Mao; Xuefang Wang; Xiupin Wang; Qi Zhang; Peiwu Li. Identification of Nutritional Components in Black Sesame Determined by Widely Targeted Metabolomics and Traditional Chinese Medicines. Molecules 2018, 23, 1180 .

AMA Style

Dandan Wang, Liangxiao Zhang, Xiaorong Huang, Xiao Wang, Ruinan Yang, Jin Mao, Xuefang Wang, Xiupin Wang, Qi Zhang, Peiwu Li. Identification of Nutritional Components in Black Sesame Determined by Widely Targeted Metabolomics and Traditional Chinese Medicines. Molecules. 2018; 23 (5):1180.

Chicago/Turabian Style

Dandan Wang; Liangxiao Zhang; Xiaorong Huang; Xiao Wang; Ruinan Yang; Jin Mao; Xuefang Wang; Xiupin Wang; Qi Zhang; Peiwu Li. 2018. "Identification of Nutritional Components in Black Sesame Determined by Widely Targeted Metabolomics and Traditional Chinese Medicines." Molecules 23, no. 5: 1180.

Review
Published: 01 April 2018 in Trends in Food Science & Technology
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With a continuous increase in population and economic development, the demand for high quality seed oils keeps increasing in China. In the last decades, many minor edible oils become increasing and popular. In this review, the chemical composition and nutritional properties of minor edible oils, including flaxseed oil, corn oil, rice bran oil, camellia oil, safflower oil, almond oil, grape seed oil, walnut oil, perilla seed oil, pumpkin seed oil, evening primrose oil, Eucommia ulmoides oliver seed oil, penoy seed oil, sea buckthorn seed oil, Acer truncatum Bunge seed oil, Torreya grandis seed oil and tomato seed oil, were summarized. The characteristic chemical compositions of these 17 kinds of minor edible oils were analyzed from fatty acid composition, phytosterols, tocopherols, total phenolic content, squalene and β-carotene contents. Different types of vegetable oils have their own specific advantages and biological activities, and appropriate vegetable oils can be selected to meet individual needs accordingly. For example, Acer truncatum Bunge seed oil contains 5.55% nervonic acid (C24:1) that can promote the repair and regeneration of nerve cells and tissues damaged, while corn oil and rice bran oil have higher contents of campesterol and total phytosterol and might be better choices for patients with high cholesterol and cardiovascular diseases. This review could benefit comprehensive understanding nutritional values of minor vegetable oils and future researching on nutrition and product development.

ACS Style

Ruinan Yang; Liangxiao Zhang; Peiwu Li; Li Yu; Jin Mao; Xiupin Wang; Qi Zhang. A review of chemical composition and nutritional properties of minor vegetable oils in China. Trends in Food Science & Technology 2018, 74, 26 -32.

AMA Style

Ruinan Yang, Liangxiao Zhang, Peiwu Li, Li Yu, Jin Mao, Xiupin Wang, Qi Zhang. A review of chemical composition and nutritional properties of minor vegetable oils in China. Trends in Food Science & Technology. 2018; 74 ():26-32.

Chicago/Turabian Style

Ruinan Yang; Liangxiao Zhang; Peiwu Li; Li Yu; Jin Mao; Xiupin Wang; Qi Zhang. 2018. "A review of chemical composition and nutritional properties of minor vegetable oils in China." Trends in Food Science & Technology 74, no. : 26-32.