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Dr. Regiane Cunha
UFPR

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0 uremic toxins
0 Chronic kidney disease (CKD)

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uremic toxins
Chronic kidney disease (CKD)

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Journal article
Published: 09 August 2021 in Journal of Ethnopharmacology
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Cancer is an inflammatory disease because carcinogenesis and tumor progression depend on intrinsic and extrinsic inflammatory pathways. Although species of the genus Aspidosperma are widely used to treat tumors, and there is ethnopharmacological evidence for traditional use of the species A. subincanum as an anti-inflammatory agent, its antineoplastic potential is unknown. To evaluate toxic effects of the indole alkaloid–rich fraction (IAF) of A. subincanum on the MCF7 cell line and identify some of the anti-inflammatory mechanisms involved. Chromatographic analyses were performed by ultra-high-performance liquid chromatography with electrospray ionization mass spectrometry, and cytotoxic and antiproliferative effects of IAF were verified by MTT and clonogenic assays. Cell cycle alterations were analyzed by measuring DNA content, while propidium iodide and acridine orange staining was performed to determine the type of induced cell death. The expression of apoptosis markers and proteins involved in cell proliferation and survival pathways was analyzed by immunoblotting, RT-qPCR, and ELISAs. Interference with redox status was investigated using a DCFH-DA probe and by measuring catalase activity. Chromatographic analyses showed that IAF is a complex mixture containing indole alkaloids. IAF selectively exerted toxic and antiproliferative effects, elevating the Bax/Bcl-xL ratio and inducing apoptosis in MCF7 cells. IAF decreased intracellular reactive oxygen species levels and increased catalase activity, while reducing the IL-8 level and suppressing COX-2 expression. IAF induces apoptosis in MCF7 cells by suppressing COX-2 expression while reducing IL-8 levels and intracellular content of reactive oxygen species.

ACS Style

Andressa F. Santos; Nádia S.R. Santos Mota; Elberth M. Schiefer; Regiane S. da Cunha; Allan M. Junkert; Andréa E.M. Stinghen; Roberto Pontarolo; Amanda R. Crisma; Almeriane M. Weffort-Santos; Rozangela C. Pedrosa; Wesley M. de Souza; Karina B. Felipe. The toxicity of Aspidosperma subincanum to MCF7 cells is related to modulation of oxidative status and proinflammatory pathways. Journal of Ethnopharmacology 2021, 281, 114512 .

AMA Style

Andressa F. Santos, Nádia S.R. Santos Mota, Elberth M. Schiefer, Regiane S. da Cunha, Allan M. Junkert, Andréa E.M. Stinghen, Roberto Pontarolo, Amanda R. Crisma, Almeriane M. Weffort-Santos, Rozangela C. Pedrosa, Wesley M. de Souza, Karina B. Felipe. The toxicity of Aspidosperma subincanum to MCF7 cells is related to modulation of oxidative status and proinflammatory pathways. Journal of Ethnopharmacology. 2021; 281 ():114512.

Chicago/Turabian Style

Andressa F. Santos; Nádia S.R. Santos Mota; Elberth M. Schiefer; Regiane S. da Cunha; Allan M. Junkert; Andréa E.M. Stinghen; Roberto Pontarolo; Amanda R. Crisma; Almeriane M. Weffort-Santos; Rozangela C. Pedrosa; Wesley M. de Souza; Karina B. Felipe. 2021. "The toxicity of Aspidosperma subincanum to MCF7 cells is related to modulation of oxidative status and proinflammatory pathways." Journal of Ethnopharmacology 281, no. : 114512.

Review
Published: 30 July 2021 in Cells
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Over the development of eukaryotic cells, intrinsic mechanisms have been developed in order to provide the ability to defend against aggressive agents. In this sense, a group of proteins plays a crucial role in controlling the production of several proteins, guaranteeing cell survival. The heat shock proteins (HSPs), are a family of proteins that have been linked to different cellular functions, being activated under conditions of cellular stress, not only imposed by thermal variation but also toxins, radiation, infectious agents, hypoxia, etc. Regarding pathological situations as seen in cardiorenal syndrome (CRS), HSPs have been shown to be important mediators involved in the control of gene transcription and intracellular signaling, in addition to be an important connector with the immune system. CRS is classified as acute or chronic and according to the first organ to suffer the injury, which can be the heart (CRS type 1 and type 2), kidneys (CRS type 3 and 4) or both (CRS type 5). In all types of CRS, the immune system, redox balance, mitochondrial dysfunction, and tissue remodeling have been the subject of numerous studies in the literature in order to elucidate mechanisms and propose new therapeutic strategies. In this sense, HSPs have been targeted by researchers as important connectors between kidney and heart. Thus, the present review has a focus to present the state of the art regarding the role of HSPs in the pathophysiology of cardiac and renal alterations, as well their role in the kidney–heart axis.

ACS Style

Carolina Junho; Carolina Azevedo; Regiane da Cunha; Ainhoa de Yurre; Emiliano Medei; Andréa Stinghen; Marcela Carneiro-Ramos. Heat Shock Proteins: Connectors between Heart and Kidney. Cells 2021, 10, 1939 .

AMA Style

Carolina Junho, Carolina Azevedo, Regiane da Cunha, Ainhoa de Yurre, Emiliano Medei, Andréa Stinghen, Marcela Carneiro-Ramos. Heat Shock Proteins: Connectors between Heart and Kidney. Cells. 2021; 10 (8):1939.

Chicago/Turabian Style

Carolina Junho; Carolina Azevedo; Regiane da Cunha; Ainhoa de Yurre; Emiliano Medei; Andréa Stinghen; Marcela Carneiro-Ramos. 2021. "Heat Shock Proteins: Connectors between Heart and Kidney." Cells 10, no. 8: 1939.

Journal article
Published: 28 March 2021 in Research, Society and Development
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Piper amalago L. is a medicinal plant traditionally used as a healing agent for wounds, burns, abscesses, boils, and insect bites. The current study aimed to evaluate the possible effects of the aqueous crude extract obtained from P. amalago leaves, in different concentrations and in different incubation times, using the in vitro model of mouse fibroblasts (3T3). The extract was tested in different concentrations at the 24 h incubation time for analysis of cell viability, cytotoxicity, proliferation, cell morphology, immunostaining, adhesion and cell spreading assays, as well as to determine the hydroxyproline concentration and activity of the metalloproteinase MMP2. Morphologically, after exposure to the concentrations of 15 and 150 µg/mL, the cells maintained the morphology, yet a greater number of cells with more expansions of the cell body and larger than the control cells were observed. The treated cell culture also showed a greater number of cells, larger cells, a greater expansion of the cell body, adherent cells spread over the substrate, and a more juxtaposed, central and spherical nucleus. The treatment induced greater cell adhesion to the polymer, fibronectin, and collagen I. Biochemical results showed a significant increase in the hydroxyproline amino acid after exposure for 96 h. The extract did not induce loss of cell viability until the concentration reached 150 µg/mL, positively modulating proliferation, morphology, adhesion, degree of spreading, and organization of microfilaments. The extract also promoted a significant increase in the hydroxyproline amino acid.

ACS Style

Vera Lucia Pereira dos Santos; Célia Regina Cavichiolo Franco; Ricardo Wagner; Caroline Dadalt Silva; Giane Favretto dos Santos; Regiane Stafim da Cunha; Andréa Emilia Marques Stinghen; Luciane Mendes Monteiro; Julia Emília Bussade; Jane Manfron Budel; Iara José de Messias-Reason. In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix. Research, Society and Development 2021, 10, 1 .

AMA Style

Vera Lucia Pereira dos Santos, Célia Regina Cavichiolo Franco, Ricardo Wagner, Caroline Dadalt Silva, Giane Favretto dos Santos, Regiane Stafim da Cunha, Andréa Emilia Marques Stinghen, Luciane Mendes Monteiro, Julia Emília Bussade, Jane Manfron Budel, Iara José de Messias-Reason. In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix. Research, Society and Development. 2021; 10 (4):1.

Chicago/Turabian Style

Vera Lucia Pereira dos Santos; Célia Regina Cavichiolo Franco; Ricardo Wagner; Caroline Dadalt Silva; Giane Favretto dos Santos; Regiane Stafim da Cunha; Andréa Emilia Marques Stinghen; Luciane Mendes Monteiro; Julia Emília Bussade; Jane Manfron Budel; Iara José de Messias-Reason. 2021. "In vitro study after exposure to the aqueous extract of Piper amalago L. shows changes of morphology, proliferation, cytoskeleton and molecules of the extracellular matrix." Research, Society and Development 10, no. 4: 1.

Journal article
Published: 22 February 2021
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This study aimed to evaluate the potential anti-inflammatory effects of vitamin D supplementation under uremic conditions, both in vivo and in vitro, and its effects on the parameters of mineral metabolism. Thirty-two hemodialysis patients were randomly assigned to receive placebo (N=14) or cholecalciferol (N=18) for six months. Serum levels of calcium, phosphate, total alkaline phosphatase, intact parathyroid hormone (iPTH), and vitamin D were measured at baseline and after three and six months. The levels of fibroblast growth factor-23 (FGF-23), interleukin-1β (IL-1β), and high-sensitivity C-reactive protein (hs-CRP) were also measured at baseline and at six months. Human monocytes were used for in vitro experiments and treated with cholecalciferol (150 nM) and uremic serum. Cell viability, reactive oxygen species (ROS) production, and cathelicidin (CAMP) expression were evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, dichloro-dihydro-fluorescein diacetate assay, and real time-quantitative polymerase chain reaction, respectively. Both patient groups were clinically and biochemically similar at baseline. After six months, the levels of vitamin D and iPTH were higher and lower, respectively, in the cholecalciferol group than in the placebo group (p<0.05). There was no significant difference between the parameters of mineral metabolism, such as IL-1β and hs-CRP levels, in both groups. Treatment with uremic serum lowered the monocyte viability (p<0.0001) and increased ROS production (p<0.01) and CAMP expression (p<0.05); these effects were counterbalanced by cholecalciferol treatment (p<0.05). Thus, cholecalciferol supplementation is an efficient strategy to ameliorate hypovitaminosis D in hemodialysis patients, but its beneficial effects on the control of secondary hyperparathyroidism are relatively unclear. Even though cholecalciferol exhibited anti-inflammatory effects in vitro, its short-term supplementation was not effective in improving the inflammatory profile of patients on hemodialysis, as indicated by the IL-1β and hs-CRP levels.

ACS Style

Paulo C Gregório; Sergio Bucharles; Regiane S da Cunha; Tárcio Braga; Ana Clara Almeida; Railson Henneberg; Andréa E M Stinghen; Fellype C Barreto. In vitro anti-inflammatory effects of vitamin D supplementation may be blurred in hemodialysis patients. 2021, 76, e1821 .

AMA Style

Paulo C Gregório, Sergio Bucharles, Regiane S da Cunha, Tárcio Braga, Ana Clara Almeida, Railson Henneberg, Andréa E M Stinghen, Fellype C Barreto. In vitro anti-inflammatory effects of vitamin D supplementation may be blurred in hemodialysis patients. . 2021; 76 ():e1821.

Chicago/Turabian Style

Paulo C Gregório; Sergio Bucharles; Regiane S da Cunha; Tárcio Braga; Ana Clara Almeida; Railson Henneberg; Andréa E M Stinghen; Fellype C Barreto. 2021. "In vitro anti-inflammatory effects of vitamin D supplementation may be blurred in hemodialysis patients." 76, no. : e1821.

Review
Published: 20 June 2020 in Toxins
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Uremic toxins can induce endothelial dysfunction in patients with chronic kidney disease (CKD). Indeed, the structure of the endothelial monolayer is damaged in CKD, and studies have shown that the uremic toxins contribute to the loss of cell–cell junctions, increasing permeability. Membrane proteins, such as transporters and receptors, can mediate the interaction between uremic toxins and endothelial cells. In these cells, uremic toxins induce oxidative stress and activation of signaling pathways, including the aryl hydrocarbon receptor (AhR), nuclear factor kappa B (NF-κB), and mitogen-activated protein kinase (MAPK) pathways. The activation of these pathways leads to overexpression of proinflammatory (e.g., monocyte chemoattractant protein-1, E-selectin) and prothrombotic (e.g., tissue factor) proteins. Uremic toxins also induce the formation of endothelial microparticles (EMPs), which can lead to the activation and dysfunction of other cells, and modulate the expression of microRNAs that have an important role in the regulation of cellular processes. The resulting endothelial dysfunction contributes to the pathogenesis of cardiovascular diseases, such as atherosclerosis and thrombotic events. Therefore, uremic toxins as well as the pathways they modulated may be potential targets for therapies in order to improve treatment for patients with CKD.

ACS Style

Regiane Stafim Da Cunha; Andressa Flores Santos; Fellype Carvalho Barreto; Andréa Emilia Marques Stinghen. How do Uremic Toxins Affect the Endothelium? Toxins 2020, 12, 412 .

AMA Style

Regiane Stafim Da Cunha, Andressa Flores Santos, Fellype Carvalho Barreto, Andréa Emilia Marques Stinghen. How do Uremic Toxins Affect the Endothelium? Toxins. 2020; 12 (6):412.

Chicago/Turabian Style

Regiane Stafim Da Cunha; Andressa Flores Santos; Fellype Carvalho Barreto; Andréa Emilia Marques Stinghen. 2020. "How do Uremic Toxins Affect the Endothelium?" Toxins 12, no. 6: 412.

Review
Published: 13 May 2019 in Toxins
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Endothelial microparticles (EMPs) are vesicles derived from cell membranes, which contain outsourced phosphatidylserine and express adhesion molecules, such as cadherin, intercellular cell adhesion molecule-1 (ICAM-1), E-selectin, and integrins. EMPs are expressed under physiological conditions and continue circulating in the plasma. However, in pathologic conditions their levels increase, and they assume a pro-inflammatory and pro-coagulant role via interactions with monocytes; these effects are related to the development of atherosclerosis. Chronic kidney dysfunction (CKD) characterizes this dysfunctional scenario through the accumulation of uremic solutes in the circulating plasma, whose toxicity is related to the development of cardiovascular diseases. Therefore, this review aims to discuss the formation of EMPs and their biological effects in the uremic environment. Data from previous research demonstrate that uremic toxins are closely associated with the activation of inflammatory biomarkers, cardiovascular dysfunction processes, and the release of EMPs. The impact of a decrease in circulating EMPs in clinical studies has not yet been evaluated. Thus, whether MPs are biochemical markers and/or therapeutic targets has yet to be established.

ACS Style

Giane Favretto; Regiane Stafim Da Cunha; Maria Aparecida Dalboni; Rodrigo Bueno De Oliveira; Fellype De Carvalho Barreto; Ziad A. Massy; Andréa Emilia Marques Stinghen. Endothelial Microparticles in Uremia: Biomarkers and Potential Therapeutic Targets. Toxins 2019, 11, 267 .

AMA Style

Giane Favretto, Regiane Stafim Da Cunha, Maria Aparecida Dalboni, Rodrigo Bueno De Oliveira, Fellype De Carvalho Barreto, Ziad A. Massy, Andréa Emilia Marques Stinghen. Endothelial Microparticles in Uremia: Biomarkers and Potential Therapeutic Targets. Toxins. 2019; 11 (5):267.

Chicago/Turabian Style

Giane Favretto; Regiane Stafim Da Cunha; Maria Aparecida Dalboni; Rodrigo Bueno De Oliveira; Fellype De Carvalho Barreto; Ziad A. Massy; Andréa Emilia Marques Stinghen. 2019. "Endothelial Microparticles in Uremia: Biomarkers and Potential Therapeutic Targets." Toxins 11, no. 5: 267.

Journal article
Published: 07 October 2018 in Toxins
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Endothelial dysfunction in uremia can result in cell-to-cell junction loss and increased permeability, contributing to cardiovascular diseases (CVD) development. This study evaluated the impact of the uremic milieu on endothelial morphology and cell junction’s proteins. We evaluated (i) serum levels of inflammatory biomarkers in a cohort of chronic kidney disease (CKD) patients and the expression of VE-cadherin and Zonula Occludens-1 (ZO-1) junction proteins on endothelial cells (ECs) of arteries removed from CKD patients during renal transplant; (ii) ECs morphology in vitro under different uremic conditions, and (iii) the impact of uremic toxins p-cresyl sulfate (PCS), indoxyl sulfate (IS), and inorganic phosphate (Pi) as well as of total uremic serum on VE-cadherin and ZO-1 gene and protein expression in cultured ECs. We found that the uremic arteries had lost their intact and continuous endothelial morphology, with a reduction in VE-cadherin and ZO-1 expression. In cultured ECs, both VE-cadherin and ZO-1 protein expression decreased, mainly after exposure to Pi and uremic serum groups. VE-cadherin mRNA expression was reduced while ZO-1 was increased after exposure to PCS, IS, Pi, and uremic serum. Our findings show that uremia alters cell-to-cell junctions leading to an increased endothelial damage. This gives a new perspective regarding the pathophysiological role of uremia in intercellular junctions and opens new avenues to improve cardiovascular outcomes in CKD patients.

ACS Style

Rayana A. P. Maciel; Regiane S. Cunha; Valentina Busato; Célia R. C. Franco; Paulo C. Gregório; Carla J. R. Dolenga; Lia S. Nakao; Ziad A. Massy; Agnès Boullier; Roberto Pecoits-Filho; Andréa E. M. Stinghen. Uremia Impacts VE-Cadherin and ZO-1 Expression in Human Endothelial Cell-to-Cell Junctions. Toxins 2018, 10, 404 .

AMA Style

Rayana A. P. Maciel, Regiane S. Cunha, Valentina Busato, Célia R. C. Franco, Paulo C. Gregório, Carla J. R. Dolenga, Lia S. Nakao, Ziad A. Massy, Agnès Boullier, Roberto Pecoits-Filho, Andréa E. M. Stinghen. Uremia Impacts VE-Cadherin and ZO-1 Expression in Human Endothelial Cell-to-Cell Junctions. Toxins. 2018; 10 (10):404.

Chicago/Turabian Style

Rayana A. P. Maciel; Regiane S. Cunha; Valentina Busato; Célia R. C. Franco; Paulo C. Gregório; Carla J. R. Dolenga; Lia S. Nakao; Ziad A. Massy; Agnès Boullier; Roberto Pecoits-Filho; Andréa E. M. Stinghen. 2018. "Uremia Impacts VE-Cadherin and ZO-1 Expression in Human Endothelial Cell-to-Cell Junctions." Toxins 10, no. 10: 404.

Journal article
Published: 05 September 2017 in Clinical Kidney Journal
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Advanced glycation end products (AGEs) have been related to the pathogenesis of cardiovascular diseases (CVD), chronic kidney disease (CKD) and diabetes mellitus. We sought to investigate the binding capacity of sevelamer to both AGEs and uremic serum in vitro and then test this pharmaceutical effect as a potential vascular anti-inflammatory strategy. AGEs were prepared by albumin glycation and characterized by absorbance and electrophoresis. Human endothelial cells were incubated in culture media containing AGEs and uremic serum with or without sevelamer. Receptor for advanced glycation end product (RAGE) expression was evaluated through immunocytochemistry and western blot to explore the interactions between AGEs and the endothelium. Inflammatory and endothelial dysfunction biomarkers, such as interleukin 6 (IL-6) and IL-8, monocyte chemoattractant protein-1 (MCP-1), plasminogen activator inhibitor-1 (PAI-1) and serum amyloid A (SAA) were also measured in cell supernatant. The chemotactic property of the supernatant was evaluated. AGEs significantly induced the expression of RAGE, inflammatory and endothelial activation biomarkers [IL-6, (P < 0.005); IL-8, MCP-1, PAI-1 and SAA (P < 0.001)] and monocyte chemotaxis as compared with controls. In addition, AGEs increased the levels of inflammatory biomarkers, which were observed after 6 h of endothelial cell incubation with uremic serum [IL-6 (P < 0.001) IL-8, MCP-1 and PAI-1 (P < 0.05)]. On the other hand, after 6 h of endothelial cell treatment with sevelamer, RAGE expression (P < 0.05) and levels of inflammatory biomarkers [IL-6 and IL-8 (P < 0.001), MCP-1 (P < 0.01), PAI-1 and SAA (P < 0.005)] significantly decreased compared with the AGEs/uremic serum treatment alone. Sevelamer decreased both endothelial expression of RAGE and endothelial dysfunction biomarkers, induced by AGEs, and uremic serum. Further studies are necessary for a better understanding of the potential protective role of sevelamer on uremic serum and AGEs-mediated endothelial dysfunction.

ACS Style

Paulo C. Gregório; Giane Favretto; Guilherme Sassaki; Regiane S. Cunha; Alessandra Becker-Finco; Roberto Pecoits-Filho; Wesley M. Souza; Fellype C. Barreto; Andréa E. M. Stinghen. Sevelamer reduces endothelial inflammatory response to advanced glycation end products. Clinical Kidney Journal 2017, 11, 89 -98.

AMA Style

Paulo C. Gregório, Giane Favretto, Guilherme Sassaki, Regiane S. Cunha, Alessandra Becker-Finco, Roberto Pecoits-Filho, Wesley M. Souza, Fellype C. Barreto, Andréa E. M. Stinghen. Sevelamer reduces endothelial inflammatory response to advanced glycation end products. Clinical Kidney Journal. 2017; 11 (1):89-98.

Chicago/Turabian Style

Paulo C. Gregório; Giane Favretto; Guilherme Sassaki; Regiane S. Cunha; Alessandra Becker-Finco; Roberto Pecoits-Filho; Wesley M. Souza; Fellype C. Barreto; Andréa E. M. Stinghen. 2017. "Sevelamer reduces endothelial inflammatory response to advanced glycation end products." Clinical Kidney Journal 11, no. 1: 89-98.

Journal article
Published: 30 May 2017 in Journal of Vascular Research
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ACS Style

Manuela Martins-Green; Yan Liu; Xue Lian Chen; Lei Wang; Alkistis Kapelouzou; Alkiviadis Kostakis; Michalis Peroulis; Michalis Katsimpoulas; Petros Moustardas; Chrysostomos V. Aravanis; Panagiotis E. Karayannakos; Dennis V. Cokkinos; Ivan Ivic; Balazs D. Fulop; Tamás Juhász; Dora Reglődi; Gábor Tóth; Hitoshi Hashimoto; Andrea Tamas; Ákos Koller; Nadine Haase; Constantin Rüder; Hannelore Haase; Stefanie Kamann; Michiyoshi Kouno; Ingo Morano; Ralf Dechend; Dietlind Zohlnhöfer; Tobias Haase; Futian Tang; Meihui Yin; Qianqian Liu; Lan Yu; Yueyan Yang; Meili Lu; Hongxin Wang; Duosheng Luo; Xianglu Rong; Jiao Guo; Roberto Pecoits-Filho; Giane Favretto; Lauro M. Souza; Paulo C. Gregório; Regiane S. Cunha; Rayana A.P. Maciel; Guilherme L. Sassaki; Maria G. Toledo; Wesley M. Souza; Andréa E.M. Stinghen; Stavros Giaglis; Satz Mengensatzproduktion; Druckerei Stückle. Membership of the ESM. Journal of Vascular Research 2017, 54, 194 -194.

AMA Style

Manuela Martins-Green, Yan Liu, Xue Lian Chen, Lei Wang, Alkistis Kapelouzou, Alkiviadis Kostakis, Michalis Peroulis, Michalis Katsimpoulas, Petros Moustardas, Chrysostomos V. Aravanis, Panagiotis E. Karayannakos, Dennis V. Cokkinos, Ivan Ivic, Balazs D. Fulop, Tamás Juhász, Dora Reglődi, Gábor Tóth, Hitoshi Hashimoto, Andrea Tamas, Ákos Koller, Nadine Haase, Constantin Rüder, Hannelore Haase, Stefanie Kamann, Michiyoshi Kouno, Ingo Morano, Ralf Dechend, Dietlind Zohlnhöfer, Tobias Haase, Futian Tang, Meihui Yin, Qianqian Liu, Lan Yu, Yueyan Yang, Meili Lu, Hongxin Wang, Duosheng Luo, Xianglu Rong, Jiao Guo, Roberto Pecoits-Filho, Giane Favretto, Lauro M. Souza, Paulo C. Gregório, Regiane S. Cunha, Rayana A.P. Maciel, Guilherme L. Sassaki, Maria G. Toledo, Wesley M. Souza, Andréa E.M. Stinghen, Stavros Giaglis, Satz Mengensatzproduktion, Druckerei Stückle. Membership of the ESM. Journal of Vascular Research. 2017; 54 (3):194-194.

Chicago/Turabian Style

Manuela Martins-Green; Yan Liu; Xue Lian Chen; Lei Wang; Alkistis Kapelouzou; Alkiviadis Kostakis; Michalis Peroulis; Michalis Katsimpoulas; Petros Moustardas; Chrysostomos V. Aravanis; Panagiotis E. Karayannakos; Dennis V. Cokkinos; Ivan Ivic; Balazs D. Fulop; Tamás Juhász; Dora Reglődi; Gábor Tóth; Hitoshi Hashimoto; Andrea Tamas; Ákos Koller; Nadine Haase; Constantin Rüder; Hannelore Haase; Stefanie Kamann; Michiyoshi Kouno; Ingo Morano; Ralf Dechend; Dietlind Zohlnhöfer; Tobias Haase; Futian Tang; Meihui Yin; Qianqian Liu; Lan Yu; Yueyan Yang; Meili Lu; Hongxin Wang; Duosheng Luo; Xianglu Rong; Jiao Guo; Roberto Pecoits-Filho; Giane Favretto; Lauro M. Souza; Paulo C. Gregório; Regiane S. Cunha; Rayana A.P. Maciel; Guilherme L. Sassaki; Maria G. Toledo; Wesley M. Souza; Andréa E.M. Stinghen; Stavros Giaglis; Satz Mengensatzproduktion; Druckerei Stückle. 2017. "Membership of the ESM." Journal of Vascular Research 54, no. 3: 194-194.

Journal article
Published: 05 May 2017 in Journal of Vascular Research
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Organic anion transporters (OATs) are involved in the uptake of uremic toxins such as p-cresyl sulfate (PCS) and indoxyl sulfate (IS), which play a role in endothelial dysfunction in patients with chronic kidney diseases (CKD). In this study, we investigated the role of OAT1 and OAT3 in the uptake of PCS and IS into human endothelial cells. PCS was synthesized via p-cresol sulfation and characterized using analytical methods. The cells were treated with PCS and IS in the absence and presence of probenecid (Pb), an OAT inhibitor. Cell viability was assessed using the MTT assay. The absorbed toxins were analyzed using chromatography, OAT expression using immunocytochemistry and western blot, and monocyte chemoattractant protein-1 (MCP-1) expression using enzyme-linked immunosorbent assay. Cell viability decreased after toxin treatment in a dose-dependent manner. PCS and IS showed significant internalization after 60 min treatment, while no internalization was observed in the presence of Pb, suggesting that OATs are involved in the transport of both toxins. Immunocytochemistry and western blot demonstrated OAT1 and OAT3 expression in endothelial cells. MCP-1 expression increased after toxins treatment but decreased after Pb treatment. PCS and IS uptake were mediated by OATs, and OAT blockage could serve as a therapeutic strategy to inhibit MCP-1 expression.

ACS Style

Giane Favretto; Lauro Souza; Paulo C. Gregório; Regiane S. Cunha; Rayana A.P. Maciel; Guilherme L. Sassaki; Maria G. Toledo; Roberto Pecoits-Filho; Wesley M. Souza; Andréa E.M. Stinghen. Role of Organic Anion Transporters in the Uptake of Protein-Bound Uremic Toxins by Human Endothelial Cells and Monocyte Chemoattractant Protein-1 Expression. Journal of Vascular Research 2017, 54, 170 -179.

AMA Style

Giane Favretto, Lauro Souza, Paulo C. Gregório, Regiane S. Cunha, Rayana A.P. Maciel, Guilherme L. Sassaki, Maria G. Toledo, Roberto Pecoits-Filho, Wesley M. Souza, Andréa E.M. Stinghen. Role of Organic Anion Transporters in the Uptake of Protein-Bound Uremic Toxins by Human Endothelial Cells and Monocyte Chemoattractant Protein-1 Expression. Journal of Vascular Research. 2017; 54 (3):170-179.

Chicago/Turabian Style

Giane Favretto; Lauro Souza; Paulo C. Gregório; Regiane S. Cunha; Rayana A.P. Maciel; Guilherme L. Sassaki; Maria G. Toledo; Roberto Pecoits-Filho; Wesley M. Souza; Andréa E.M. Stinghen. 2017. "Role of Organic Anion Transporters in the Uptake of Protein-Bound Uremic Toxins by Human Endothelial Cells and Monocyte Chemoattractant Protein-1 Expression." Journal of Vascular Research 54, no. 3: 170-179.

Articles
Published: 01 September 2016 in Ciência & Educação (Bauru)
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Resumo: A formação inicial de professores inclui o estágio em docência, que proporciona a vivência do cotidiano escolar. A partir disso, o presente estudo investiga a experiência de quatro estagiários de professores de Biologia em diferentes turmas de Ensino Médio da Educação Básica. Para tanto, cada estagiário produziu relatos que foram submetidos à análise da ação docente utilizando o instrumento Matriz 3x3 baseado no sistema didático de Chevallard e na gestão das relações com o saber de Charlot, proposto por Arruda, Lima e Passos. O estágio foi marcado pelo uso de diferentes práticas metodológicas, tais como criação e montagem de modelos didáticos, vídeos e gravuras, de forma que os estagiários puderam refletir sobre a prática docente e sobre as relações existentes no âmbito da escola.

ACS Style

Regiane Stafim Da Cunha; Marina Rosa Stec Dos Santos; Jaqueline Dittrich; Maiara Vicentini; Liege Da Silva Oliveira Stavis; Christiane Gioppo Marques Da Cruz. Formação inicial docente e suas relações dentro do âmbito escolar. Ciência & Educação (Bauru) 2016, 22, 585 -596.

AMA Style

Regiane Stafim Da Cunha, Marina Rosa Stec Dos Santos, Jaqueline Dittrich, Maiara Vicentini, Liege Da Silva Oliveira Stavis, Christiane Gioppo Marques Da Cruz. Formação inicial docente e suas relações dentro do âmbito escolar. Ciência & Educação (Bauru). 2016; 22 (3):585-596.

Chicago/Turabian Style

Regiane Stafim Da Cunha; Marina Rosa Stec Dos Santos; Jaqueline Dittrich; Maiara Vicentini; Liege Da Silva Oliveira Stavis; Christiane Gioppo Marques Da Cruz. 2016. "Formação inicial docente e suas relações dentro do âmbito escolar." Ciência & Educação (Bauru) 22, no. 3: 585-596.