This page has only limited features, please log in for full access.

Unclaimed
Peter Müller
Friedrich Schiller University, Matthias-Schleiden-Institut, AG Bakteriengenetik

Basic Info

Basic Info is private.

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Preprint content
Published: 15 May 2020
Reads 0
Downloads 0

SummaryHere, we describe SR7, a dual-function antisense RNA from the Bacillus subtilis chromosome. This RNA was earlier published as the SigB-dependent regulatory RNA S1136 and reported to reduce the amount of the small ribosomal subunit under ethanol stress. We found that the 5’ portion of SR7 encodes a small protein composed of 39 amino acids which we designated SR7P. It is translated from a 185 nt SigB-dependent mRNA under five different stress conditions and a longer SigB-independent RNA constitutively. Two- to three-fold higher amounts of SR7P were detected in B. subtilis cells exposed to salt, ethanol or heat stress. Co-elution experiments with SR7PC-FLAG and Far-Western blotting demonstrated that SR7P interacts with the glycolytic enzyme enolase. Enolase is a scaffolding component of the B. subtilis degradosome where it interacts with RNase Y and phosphofructokinase PfkA. We found that SR7P increases the amount of RNase Y bound to enolase without affecting PfkA. RNA does not bridge the SR7P-enolase-RNase Y interaction. In vitro-degradation assays with the known RNase Y substrates yitJ and rpsO mRNA revealed enhanced enzymatic activity of enolase-bound RNase Y in the presence of SR7P. Northern blots showed a major effect of enolase and a minor effect of SR7P on the half-life of rpsO mRNA indicating a fine-tuning role of SR7P in RNA degradation. Moreover, SR7P impacts survival of B. subtilis under stress conditions. We suggest that the SR7P-dependent modification of the degradosome affects targets in different physiological pathways.

ACS Style

Inam Ul Haq; Peter Müller; Sabine Brantl. SR7 - a dual function antisense RNA from Bacillus subtilis. 2020, 1 .

AMA Style

Inam Ul Haq, Peter Müller, Sabine Brantl. SR7 - a dual function antisense RNA from Bacillus subtilis. . 2020; ():1.

Chicago/Turabian Style

Inam Ul Haq; Peter Müller; Sabine Brantl. 2020. "SR7 - a dual function antisense RNA from Bacillus subtilis." , no. : 1.

Review
Published: 09 May 2019 in Toxins
Reads 0
Downloads 0

Toxin–antitoxin (TA) systems were originally discovered as plasmid maintenance systems in a multitude of free-living bacteria, but were afterwards found to also be widespread in bacterial chromosomes. TA loci comprise two genes, one coding for a stable toxin whose overexpression kills the cell or causes growth stasis, and the other coding for an unstable antitoxin that counteracts toxin action. Of the currently known six types of TA systems, in Bacillus subtilis, so far only type I and type II TA systems were found, all encoded on the chromosome. Here, we review our present knowledge of these systems, the mechanisms of antitoxin and toxin action, and the regulation of their expression, and we discuss their evolution and possible physiological role.

ACS Style

Sabine Brantl; Peter Müller. Toxin–Antitoxin Systems in Bacillus subtilis. Toxins 2019, 11, 262 .

AMA Style

Sabine Brantl, Peter Müller. Toxin–Antitoxin Systems in Bacillus subtilis. Toxins. 2019; 11 (5):262.

Chicago/Turabian Style

Sabine Brantl; Peter Müller. 2019. "Toxin–Antitoxin Systems in Bacillus subtilis." Toxins 11, no. 5: 262.