This page has only limited features, please log in for full access.

Dr. Yoko Aida
Laboratory of Viral Infectious Diseases, Department of Medical Genome Sciences, Graduate School of Frontier Science, The University of Tokyo

Basic Info


Research Keywords & Expertise

0 Influenza
0 MHC
0 Retroviruses
0 HIV and AIDS
0 BLV

Fingerprints

BLV
MHC
Influenza
Retroviruses

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 16 August 2021 in Journal of Virological Methods
Reads 0
Downloads 0

The bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, the most common neoplastic disease in cattle. We previously developed the quantitative real-time PCR (qPCR) assay to measure the proviral loads of BLV using coordination of common motif (CoCoMo) degenerate primers. We here found four single mutations within the probe region of the original BLV-CoCoMo-qPCR assay, three of which have negative impact on its sensitivity in the probe sequences of the long terminal regions of the BLV-CoCoMo-qPCR-2 assay, using genomic DNA from 887 cows from 27 BLV-positive farms via a nationwide survey conducted in 2011 and 2017 in Japan. Therefore, the modified probes were designed to completely match the three BLV mutant strains identified here. Moreover, we examined the optimum ratio of the concentration to be mixed with the wild type and three new BLV TaqMan probes were designed here using genomic DNAs extracted from cattle naturally infected with the wild type BLV strain and three mutant strains. Finally, we successfully established an improved assay maintained the original sensitivity and reproducibility and can detect novel BLV strains.

ACS Style

Liushiqi Borjigin; Shuji Yoneyama; Susumu Saito; Meripet Polat; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Risa Yamanaka; Anna Yasui; Munehito Mimura; Hironobu Murakami; Shin-Nosuke Takeshima; Yoko Aida. A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains. Journal of Virological Methods 2021, 297, 114264 .

AMA Style

Liushiqi Borjigin, Shuji Yoneyama, Susumu Saito, Meripet Polat, Michihito Inokuma, Yasuo Shinozaki, Naoko Tanaka, Risa Yamanaka, Anna Yasui, Munehito Mimura, Hironobu Murakami, Shin-Nosuke Takeshima, Yoko Aida. A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains. Journal of Virological Methods. 2021; 297 ():114264.

Chicago/Turabian Style

Liushiqi Borjigin; Shuji Yoneyama; Susumu Saito; Meripet Polat; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Risa Yamanaka; Anna Yasui; Munehito Mimura; Hironobu Murakami; Shin-Nosuke Takeshima; Yoko Aida. 2021. "A novel real time PCR assay for bovine leukemia virus detection using mixed probes and degenerate primers targeting novel BLV strains." Journal of Virological Methods 297, no. : 114264.

Article
Published: 05 July 2021 in Scientific Reports
Reads 0
Downloads 0

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been a pandemic threat worldwide and causes severe health and economic burdens. Contaminated environments, such as personal items and room surfaces, are considered to have virus transmission potential. Ultraviolet C (UVC) light has demonstrated germicidal ability and removes environmental contamination. UVC has inactivated SARS-CoV-2; however, the underlying mechanisms are not clear. It was confirmed here that UVC 253.7 nm, with a dose of 500 μW/cm2, completely inactivated SARS-CoV-2 in a time-dependent manner and reduced virus infectivity by 10–4.9-fold within 30 s. Immunoblotting analysis for viral spike and nucleocapsid proteins showed that UVC treatment did not damage viral proteins. The viral particle morphology remained intact even when the virus completely lost infectivity after UVC irradiation, as observed by transmission electronic microscopy. In contrast, UVC irradiation-induced genome damage was identified using the newly developed long reverse-transcription quantitative-polymerase chain reaction (RT-qPCR) assay, but not conventional RT-qPCR. The six developed long RT-PCR assays that covered the full-length viral genome clearly indicated a negative correlation between virus infectivity and UVC irradiation-induced genome damage (R2 ranging from 0.75 to 0.96). Altogether, these results provide evidence that UVC inactivates SARS-CoV-2 through the induction of viral genome damage.

ACS Style

Chieh-Wen Lo; Ryosuke Matsuura; Kazuki Iimura; Satoshi Wada; Atsushi Shinjo; Yoshimi Benno; Masaru Nakagawa; Masami Takei; Yoko Aida. UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins. Scientific Reports 2021, 11, 1 -11.

AMA Style

Chieh-Wen Lo, Ryosuke Matsuura, Kazuki Iimura, Satoshi Wada, Atsushi Shinjo, Yoshimi Benno, Masaru Nakagawa, Masami Takei, Yoko Aida. UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins. Scientific Reports. 2021; 11 (1):1-11.

Chicago/Turabian Style

Chieh-Wen Lo; Ryosuke Matsuura; Kazuki Iimura; Satoshi Wada; Atsushi Shinjo; Yoshimi Benno; Masaru Nakagawa; Masami Takei; Yoko Aida. 2021. "UVC disinfects SARS-CoV-2 by induction of viral genome damage without apparent effects on viral morphology and proteins." Scientific Reports 11, no. 1: 1-11.

Journal article
Published: 20 May 2021 in Viruses
Reads 0
Downloads 0

SARS-CoV-2 is the causative agent of COVID-19, which is a global pandemic. SARS-CoV-2 is transmitted rapidly via contaminated surfaces and aerosols, emphasizing the importance of environmental disinfection to block the spread of virus. Ultraviolet C radiation and chemical compounds are effective for SARS-CoV-2 disinfection, but can only be applied in the absence of humans due to their toxicities. Therefore, development of disinfectants that can be applied in working spaces without evacuating people is needed. Here we showed that TiO2-mediated photocatalytic reaction inactivates SARS-CoV-2 in a time-dependent manner and decreases its infectivity by 99.9% after 20 min and 120 min of treatment in aerosol and liquid, respectively. The mechanistic effects of TiO2 photocatalyst on SARS-CoV-2 virion included decreased total observed virion count, increased virion size, and reduced particle surface spike structure, as determined by transmission electron microscopy. Damage to viral proteins and genome was further confirmed by western blotting and RT-qPCR, respectively. The multi-antiviral effects of TiO2-mediated photocatalytic reaction implies universal disinfection potential for different infectious agents. Notably, TiO2 has no adverse effects on human health, and therefore, TiO2-induced photocatalytic reaction is suitable for disinfection of SARS-CoV-2 and other emerging infectious disease-causing agents in human habitation.

ACS Style

Ryosuke Matsuura; Chieh-Wen Lo; Satoshi Wada; Junichi Somei; Heihachiro Ochiai; Takeharu Murakami; Norihito Saito; Takayo Ogawa; Atsushi Shinjo; Yoshimi Benno; Masaru Nakagawa; Masami Takei; Yoko Aida. SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO2 Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein. Viruses 2021, 13, 942 .

AMA Style

Ryosuke Matsuura, Chieh-Wen Lo, Satoshi Wada, Junichi Somei, Heihachiro Ochiai, Takeharu Murakami, Norihito Saito, Takayo Ogawa, Atsushi Shinjo, Yoshimi Benno, Masaru Nakagawa, Masami Takei, Yoko Aida. SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO2 Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein. Viruses. 2021; 13 (5):942.

Chicago/Turabian Style

Ryosuke Matsuura; Chieh-Wen Lo; Satoshi Wada; Junichi Somei; Heihachiro Ochiai; Takeharu Murakami; Norihito Saito; Takayo Ogawa; Atsushi Shinjo; Yoshimi Benno; Masaru Nakagawa; Masami Takei; Yoko Aida. 2021. "SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO2 Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein." Viruses 13, no. 5: 942.

Brief communication
Published: 25 April 2021 in HLA
Reads 0
Downloads 0

Bovine leukemia virus (BLV) causes enzootic bovine leucosis. Host genetic heterozygosity at the major histocompatibility complex can enhance the ability to combat infectious diseases. However, heterozygote advantage is loci specific and depends on disease type. Bovine leukocyte antigen (BoLA)‐DRB3 polymorphisms are related with BLV‐infection outcome; however, whether BoLA‐DRB3 heterozygotes have an advantage against BLV‐induced lymphoma and proviral load (PVL) remains unclear. By analyzing 1567 BLV‐infected individuals, we found that BoLA‐DRB3 heterozygous status was significantly associated with lymphoma resistance irrespective of cattle breeds (p < 0.0001). Similarly, decreased PVL was observed in BoLA‐DRB3 heterozygotes (p = 0.0407 for Holstein cows; p = 0.0889 for Japanese Black cattle). Our report provides first evidence of BoLA‐DRB3 heterozygote advantage against BLV infection outcome.

ACS Style

Chieh‐Wen Lo; Shin‐Nosuke Takeshima; Satoshi Wada; Yasunobu Matsumoto; Yoko Aida. Bovine major histocompatibility complex ( BoLA ) heterozygote advantage against the outcome of bovine leukemia virus infection. HLA 2021, 98, 132 -139.

AMA Style

Chieh‐Wen Lo, Shin‐Nosuke Takeshima, Satoshi Wada, Yasunobu Matsumoto, Yoko Aida. Bovine major histocompatibility complex ( BoLA ) heterozygote advantage against the outcome of bovine leukemia virus infection. HLA. 2021; 98 (2):132-139.

Chicago/Turabian Style

Chieh‐Wen Lo; Shin‐Nosuke Takeshima; Satoshi Wada; Yasunobu Matsumoto; Yoko Aida. 2021. "Bovine major histocompatibility complex ( BoLA ) heterozygote advantage against the outcome of bovine leukemia virus infection." HLA 98, no. 2: 132-139.

Journal article
Published: 22 April 2021 in Pathogens
Reads 0
Downloads 0

Perinatal transmission plays a critical role in the spread of bovine leukemia virus (BLV) infection in cattle herds. In the Holstein breed, we previously identified BLV resistant and susceptible bovine leukocyte antigen (BoLA)-DRB3 alleles, including BoLA-DRB3*009:02 and *014:01:01 with a low BLV proviral load (PVL), and *015:01 and *012:01 with a high PVL. Here, we evaluated the perinatal BLV transmission risk in dams with different BoLA-DRB3 alleles. BoLA-DRB3 alleles of 120 dam-calf pairs from five dairy farms in Japan were identified; their PVL was quantified using the BLV-Coordination of Common Motifs (CoCoMo)-qPCR-2 assay. Ninety-six dams were BLV-positive, and 29 gave birth to BLV-infected calves. Perinatal transmission frequency was 19% in dams with resistant alleles suppressed to a low PVL level, and 38% and 25% in dams with susceptible and neutral alleles that maintained high PVL levels, respectively. Notably, all calves with resistant alleles were BLV free, whereas 30% of calves with susceptible genes were infected. Thus, vertical transmission risk was extremely lower for dams and calves with resistant alleles compared to those with susceptible alleles. Our results can inform the development of effective BLV eradication programs under field conditions by providing necessary data to allow for optimal selection of dams for breeding.

ACS Style

Liushiqi Borjigin; Chieh-Wen Lo; Lanlan Bai; Rania Hamada; Hirotaka Sato; Shuji Yoneyama; Anna Yasui; Sohei Yasuda; Risa Yamanaka; Munehito Mimura; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Shin-Nosuke Takeshima; Yoko Aida. Risk Assessment of Bovine Major Histocompatibility Complex Class II DRB3 Alleles for Perinatal Transmission of Bovine Leukemia Virus. Pathogens 2021, 10, 502 .

AMA Style

Liushiqi Borjigin, Chieh-Wen Lo, Lanlan Bai, Rania Hamada, Hirotaka Sato, Shuji Yoneyama, Anna Yasui, Sohei Yasuda, Risa Yamanaka, Munehito Mimura, Michihito Inokuma, Yasuo Shinozaki, Naoko Tanaka, Shin-Nosuke Takeshima, Yoko Aida. Risk Assessment of Bovine Major Histocompatibility Complex Class II DRB3 Alleles for Perinatal Transmission of Bovine Leukemia Virus. Pathogens. 2021; 10 (5):502.

Chicago/Turabian Style

Liushiqi Borjigin; Chieh-Wen Lo; Lanlan Bai; Rania Hamada; Hirotaka Sato; Shuji Yoneyama; Anna Yasui; Sohei Yasuda; Risa Yamanaka; Munehito Mimura; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Shin-Nosuke Takeshima; Yoko Aida. 2021. "Risk Assessment of Bovine Major Histocompatibility Complex Class II DRB3 Alleles for Perinatal Transmission of Bovine Leukemia Virus." Pathogens 10, no. 5: 502.

Journal article
Published: 06 April 2021 in Pathogens
Reads 0
Downloads 0

Bovine leukemia virus (BLV) causes enzootic bovine leucosis, a malignant B-cell lymphoma in cattle. The DNA sequence polymorphisms of bovine leukocyte antigen (BoLA)-DRB3 have exhibited a correlation with BLV-induced lymphoma in Holstein cows. However, the association may vary between different cattle breeds. Furthermore, little is known about the relationship between BLV-induced lymphoma and DRB3 at the amino acid and structural diversity levels. Here, we comprehensively analyzed the correlation between BLV-induced lymphoma and DRB3 at DNA, amino acid, and binding pocket property levels, using 106 BLV-infected asymptomatic and 227 BLV-induced lymphoma Japanese black cattle samples. DRB3*011:01 was identified as a resistance allele, whereas DRB3*005:02 and DRB3*016:01 were susceptibility alleles. Amino acid association studies showed that positions 9, 11, 13, 26, 30, 47, 57, 70, 71, 74, 78, and 86 were associated with lymphoma susceptibility. Structure and electrostatic charge modeling further indicated that binding pocket 9 of resistance DRB3 was positively charged. In contrast, alleles susceptible to lymphoma were neutrally charged. Altogether, this is the first association study of BoLA-DRB3 polymorphisms with BLV-induced lymphoma in Japanese black cattle. In addition, our results further contribute to understanding the mechanisms regarding how BoLA-DRB3 polymorphisms mediate susceptibility to BLV-induced lymphoma.

ACS Style

Chieh-Wen Lo; Shin-Nosuke Takeshima; Kosuke Okada; Etsuko Saitou; Tatsuo Fujita; Yasunobu Matsumoto; Satoshi Wada; Hidetoshi Inoko; Yoko Aida. Association of Bovine Leukemia Virus-Induced Lymphoma with BoLA-DRB3 Polymorphisms at DNA, Amino Acid, and Binding Pocket Property Levels. Pathogens 2021, 10, 437 .

AMA Style

Chieh-Wen Lo, Shin-Nosuke Takeshima, Kosuke Okada, Etsuko Saitou, Tatsuo Fujita, Yasunobu Matsumoto, Satoshi Wada, Hidetoshi Inoko, Yoko Aida. Association of Bovine Leukemia Virus-Induced Lymphoma with BoLA-DRB3 Polymorphisms at DNA, Amino Acid, and Binding Pocket Property Levels. Pathogens. 2021; 10 (4):437.

Chicago/Turabian Style

Chieh-Wen Lo; Shin-Nosuke Takeshima; Kosuke Okada; Etsuko Saitou; Tatsuo Fujita; Yasunobu Matsumoto; Satoshi Wada; Hidetoshi Inoko; Yoko Aida. 2021. "Association of Bovine Leukemia Virus-Induced Lymphoma with BoLA-DRB3 Polymorphisms at DNA, Amino Acid, and Binding Pocket Property Levels." Pathogens 10, no. 4: 437.

Preprint content
Published: 02 November 2020
Reads 0
Downloads 0

Background: The bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, the most common neoplastic disease in cattle. The proviral load (PVL) is an important index for estimating disease progression. Previously, we developed a quantitative real-time PCR (qPCR) assay to measure the PVL of BLV using coordination of common motif (CoCoMo) degenerate primers that can amplify all known BLV strains. However, mutations, which potentially affect the detection ability, have been recently reported in the probe sequences of the long terminal regions (LTRs) of the BLV-CoCoMo-qPCR-2 assay. Here, we developed a new strategy to overcome these newly generated mutations located in the probe regions of this assay.Methods: We collected genomic DNA from 887 cows from 27 BLV-positive farms, using a nationwide survey conducted in 2011 and 2017 in Japan. BLV variants were investigated by quantifying the provirus using BLV-CoCoMo-qPCR-2 targeting the BLV LTR gene and the TaKaRa Cycleave PCR system targeting the BLV tax gene. Additionally, we sequenced the partial BLV LTR gene. The modified probes were designed to completely match the three BLV variants identified here, and the modified assay was established using mixed probes.Results: We found four single mutations within the probe region of the original BLV-CoCoMo-qPCR-2 assay, three of which negatively affected its sensitivity. Furthermore, we examined the optimum ratio of the concentration to be mixed with the wild type and three new BLV TaqMan probes were designed here using genomic DNAs extracted from cattle infected with the wild-type BLV strain and cattle infected with variants. Hence, we successfully established an improved BLV-CoCoMo-qPCR-3 assay that uses mixed probes corresponding to all three BLV variants. Conclusions: To overcome the loss of assay sensitivity due to newly emerging variants, we have established the BLV-CoCoMo-qPCR-3 assay that could amplify all BLV strains using newly designed mixed probes in addition to degenerate primers that were previously designed in our original assay. Our proposed method maintained the original sensitivity and reproducibility and can detect all mutant strains; thus, it is a useful tool to prevent the spread of BLV infections, especially those caused by newly emerging variants.

ACS Style

Liushiqi Borjigin; Shuji Yoneyama; Susumu Saito; Polat Meripet; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Risa Yamanaka; Anna Yasui; Munehito Mimura; Hironobu Murakami; Shin-Nosuke Takeshima; Yoko Aida. BLV-CoCoMo-qPCR-3: Improved BLV-CoCoMo-qPCR for Bovine Leukemia Virus Detection by Mixing Probes Targeting all BLV Variants. 2020, 1 .

AMA Style

Liushiqi Borjigin, Shuji Yoneyama, Susumu Saito, Polat Meripet, Michihito Inokuma, Yasuo Shinozaki, Naoko Tanaka, Risa Yamanaka, Anna Yasui, Munehito Mimura, Hironobu Murakami, Shin-Nosuke Takeshima, Yoko Aida. BLV-CoCoMo-qPCR-3: Improved BLV-CoCoMo-qPCR for Bovine Leukemia Virus Detection by Mixing Probes Targeting all BLV Variants. . 2020; ():1.

Chicago/Turabian Style

Liushiqi Borjigin; Shuji Yoneyama; Susumu Saito; Polat Meripet; Michihito Inokuma; Yasuo Shinozaki; Naoko Tanaka; Risa Yamanaka; Anna Yasui; Munehito Mimura; Hironobu Murakami; Shin-Nosuke Takeshima; Yoko Aida. 2020. "BLV-CoCoMo-qPCR-3: Improved BLV-CoCoMo-qPCR for Bovine Leukemia Virus Detection by Mixing Probes Targeting all BLV Variants." , no. : 1.

Journal article
Published: 30 October 2020 in Pathogens
Reads 0
Downloads 0

Bovine leukemia virus (BLV) causes enzootic bovine leukosis, a malignant form of B-cell lymphoma, and is closely related to human T-cell leukemia viruses. We investigated whether BLV infection affects host genes associated with DNA mismatch repair (MMR). Next-generation sequencing of blood samples from five calves experimentally infected with BLV revealed the highest expression levels of seven MMR genes (EXO1, UNG, PCNA, MSH2, MSH3, MSH6, and PMS2) at the point of peak proviral loads (PVLs). Furthermore, MMR gene expression was only upregulated in cattle with higher PVLs. In particular, the expression levels of MSH2, MSH3, and UNG positively correlated with PVL in vivo. The expression levels of all seven MMR genes in pig kidney-15 cells and the levels of PMS2 and EXO1 in HeLa cells also increased tendencies after transient transfection with a BLV infectious clone. Moreover, MMR gene expression levels were significantly higher in BLV-expressing cell lines compared with those in the respective parental cell lines. Expression levels of MSH2 and EXO1 in BLV-infected cattle with lymphoma were significantly lower and higher, respectively, compared with those in infected cattle in vivo. These results reveal that BLV infection affects MMR gene expression, offering new candidate markers for lymphoma diagnosis.

ACS Style

Lanlan Bai; Tomoya Hirose; Wlaa Assi; Satoshi Wada; Shin-Nosuke Takeshima; Yoko Aida. Bovine Leukemia Virus Infection Affects Host Gene Expression Associated with DNA Mismatch Repair. Pathogens 2020, 9, 909 .

AMA Style

Lanlan Bai, Tomoya Hirose, Wlaa Assi, Satoshi Wada, Shin-Nosuke Takeshima, Yoko Aida. Bovine Leukemia Virus Infection Affects Host Gene Expression Associated with DNA Mismatch Repair. Pathogens. 2020; 9 (11):909.

Chicago/Turabian Style

Lanlan Bai; Tomoya Hirose; Wlaa Assi; Satoshi Wada; Shin-Nosuke Takeshima; Yoko Aida. 2020. "Bovine Leukemia Virus Infection Affects Host Gene Expression Associated with DNA Mismatch Repair." Pathogens 9, no. 11: 909.

Preprint content
Published: 17 September 2020
Reads 0
Downloads 0

Background: Human immunodeficiency virus type 1 (HIV-1) modulates the host cell cycle. The HIV-1 accessory protein Vpr arrests the cell cycle at G2 phase, which is important for efficient viral replication in dividing CD4+ T cells, because the transcriptional activity of the HIV-1 long terminal repeat is most active in G2 phase. Additionally, Vpr-mediated G2 arrest likely correlates with enhanced HIV-1 infection in monocyte-derived macrophages.Results: Here, we screened small-interfering RNA to reveal candidates that suppress Vpr-induced G2 arrest and identified Huntingtin-interacting protein 1 (HIP1) as both directly interacting with Vpr and required for efficient G2 arrest. Interestingly, HIP1 was not essential for Vpr-induced DNA double-strand breaks, which are required for activation of the DNA-damage checkpoint and G2 arrest. Furthermore, HIP1 knockdown suppressed HIV-1 infection in monocyte-derived macrophages.Conclusions: These results suggest that HIP1 operates in the downstream step(s) of DNA-damage induction to promote Vpr-induced G2 arrest and enhances HIV-1 infection in macrophages.

ACS Style

Tomoyuki Murakami; Nopporn Chutiwitoonchai; Masami Takei; Yoko Aida. Huntingtin-interacting Protein 1 Promotes Vpr-induced G2 Arrest and HIV-1 Infection in Macrophages. 2020, 1 .

AMA Style

Tomoyuki Murakami, Nopporn Chutiwitoonchai, Masami Takei, Yoko Aida. Huntingtin-interacting Protein 1 Promotes Vpr-induced G2 Arrest and HIV-1 Infection in Macrophages. . 2020; ():1.

Chicago/Turabian Style

Tomoyuki Murakami; Nopporn Chutiwitoonchai; Masami Takei; Yoko Aida. 2020. "Huntingtin-interacting Protein 1 Promotes Vpr-induced G2 Arrest and HIV-1 Infection in Macrophages." , no. : 1.

Original research article
Published: 10 September 2020 in Frontiers in Veterinary Science
Reads 0
Downloads 0

Bovine leukemia virus (BLV) causes enzootic bovine leukosis (EBL), the most common neoplastic disease in cattle worldwide. The first EBL outbreak in Egypt was reported in 1997. To date, there are few studies regarding BLV diagnosis using only serological detection and no studies investigating the distribution of BLV provirus, which is the retroviral genome integrated into the host genome, in Egypt. The genetic characteristics of Egyptian BLV strains are also unknown. Therefore, we aimed to detect BLV provirus and determine BLV genetic variability among dairy cattle in Egypt. We collected 270 blood samples of dairy cattle from 24 farms located in five provinces in Egypt. Out of the 270 samples, 58 (21.5%) were positive for BLV provirus. Phylogenetic analysis based on 18 420-bp selected sequences out of 50 isolates of the BLV env-gp51 gene demonstrated that Egyptian BLV isolates were clustered into genotype-1 and-4, among 11 genotypes detected worldwide. Furthermore, phylogenetic analysis and alignment of the 501-bp sequence of the env-gp51 gene revealed that at least six genetically different strains are present in Egypt. Genotype-1 isolates comprised four different strains (G1-a, G1-b, G1-c, and G1-d) and genotype-4 isolates included two different strains (G4-x and G4-y). Moreover, in one farm with 100% infection rate, we identified three isolates of G1-a strain, 35 isolates of G4-x strain, and two isolates of G4-y strain. Overall, this study provides the new report on molecular prevalence of BLV in Egypt and records the coexistence of BLV genotype-1 and-4 in Egyptian cattle.

ACS Style

Rania Hamada; Samy Metwally; Meripet Polat; Liushiqi Borjigin; Alsagher O. Ali; A. A. A. Abdel-Hady; Adel E. A. Mohamed; Satoshi Wada; Yoko Aida. Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle. Frontiers in Veterinary Science 2020, 7, 608 .

AMA Style

Rania Hamada, Samy Metwally, Meripet Polat, Liushiqi Borjigin, Alsagher O. Ali, A. A. A. Abdel-Hady, Adel E. A. Mohamed, Satoshi Wada, Yoko Aida. Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle. Frontiers in Veterinary Science. 2020; 7 ():608.

Chicago/Turabian Style

Rania Hamada; Samy Metwally; Meripet Polat; Liushiqi Borjigin; Alsagher O. Ali; A. A. A. Abdel-Hady; Adel E. A. Mohamed; Satoshi Wada; Yoko Aida. 2020. "Detection and Molecular Characterization of Bovine Leukemia Virus in Egyptian Dairy Cattle." Frontiers in Veterinary Science 7, no. : 608.

Research article
Published: 01 September 2020 in BMC Genetics
Reads 0
Downloads 0

Myanmar cattle populations predominantly consist of native cattle breeds (Pyer Sein and Shwe), characterized by their geographical location and coat color, and the Holstein-Friesian crossbreed, which is highly adapted to the harsh tropical climates of this region. Here, we analyzed the diversity and genetic structure of the BoLA-DRB3 gene, a genetic locus that has been linked to the immune response, in Myanmar cattle populations. Blood samples (n = 294) were taken from two native breeds (Pyer Sein, n = 163 and Shwe Ni, n = 69) and a cattle crossbreed (Holstein-Friesian, n = 62) distributed across six regions of Myanmar (Bago, n = 38; Sagaing, n = 77; Mandalay, n = 46; Magway, n = 46; Kayin, n = 43; Yangon, n = 44). In addition, a database that included 2428 BoLA-DRB3 genotypes from European (Angus, Hereford, Holstein, Shorthorn, Overo Negro, Overo Colorado, and Jersey), Zebuine (Nellore, Brahman and Gir), Asian Native from Japan and Philippine and Latin-American Creole breeds was also included. Furthermore, the information from the IPD–MHC database was also used in the present analysis. DNA was genotyped using the sequence-based typing method. DNA electropherograms were analyzed using the Assign 400ATF software. We detected 71 distinct alleles, including three new variants for the BoLA-DRB3 gene. Venn analysis showed that 11 of these alleles were only detected in Myanmar native breeds and 26 were only shared with Asian native and/or Zebu groups. The number of alleles ranged from 33 in Holstein-Friesians to 58 in Pyer Seins, and the observed versus unbiased expected heterozygosity were higher than 0.84 in all the three the populations analyzed. The FST analysis showed a low level of genetic differentiation between the two Myanmar native breeds (FST = 0.003), and between these native breeds and the Holstein-Friesians (FST < 0.021). The average FST value for all the Myanmar Holstein-Friesian crossbred and Myanmar native populations was 0.0136 and 0.0121, respectively. Principal component analysis (PCA) and tree analysis showed that Myanmar native populations grouped in a narrow cluster that diverged clearly from the Holstein-Friesian populations. Furthermore, the BoLA-DRB3 allele frequencies suggested that while some Myanmar native populations from Bago, Mandalay and Yangon regions were more closely related to Zebu breeds (Gir and Brahman), populations from Kayin, Magway and Sagaing regions were more related to the Philippines native breeds. On the contrary, PCA showed that the Holstein-Friesian populations demonstrated a high degree of dispersion, which is likely the result of the different degrees of native admixture in these populations. This study is the first to report the genetic diversity of the BoLA-DRB3 gene in two native breeds and one exotic cattle crossbreed from Myanmar. The results obtained contribute to our understanding of the genetic diversity and distribution of BoLA-DRB3 gene alleles in Myanmar, and increases our knowledge of the worldwide variability of cattle BoLA-DRB3 genes, an important locus for immune response and protection against pathogens.

ACS Style

Guillermo Giovambattista; Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Si Thu Hein; Hla Hla Moe; Shin-Nosuke Takeshima; Yoko Aida. Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar. BMC Genetics 2020, 21, 1 -17.

AMA Style

Guillermo Giovambattista, Kyaw Kyaw Moe, Meripet Polat, Liushiqi Borjigin, Si Thu Hein, Hla Hla Moe, Shin-Nosuke Takeshima, Yoko Aida. Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar. BMC Genetics. 2020; 21 (1):1-17.

Chicago/Turabian Style

Guillermo Giovambattista; Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Si Thu Hein; Hla Hla Moe; Shin-Nosuke Takeshima; Yoko Aida. 2020. "Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar." BMC Genetics 21, no. 1: 1-17.

Preprint content
Published: 20 August 2020
Reads 0
Downloads 0

Background: Myanmar cattle populations predominantly consist of native cattle breeds (Pyer Sein and Shwe), characterized by their geographical location and coat color, and the Holstein-Friesian crossbreed, which is highly adapted to the harsh tropical climates of this region. Here, we analyzed the diversity and genetic structure of the BoLA-DRB3 gene, a genetic locus that has been linked to the immune response, in Myanmar cattle populations.Methods: Blood samples (n=294) were taken from two native breeds (Pyer Sein, n=163 and Shwe Ni, n=69) and a cattle crossbreed (Holstein-Friesian, n=62) distributed across six regions of Myanmar (Bago, n=38; Sagaing, n=77; Mandalay, n=46; Magway, n=46; Kayin, n=43; Yangon, n=44). In addition, a database that included 2,428 BoLA-DRB3 genotypes from European (Angus, Hereford, Holstein, Shorthorn, Overo Negro, Overo Colorado, and Jersey), Zebuine (Nellore, Brahman and Gir), Asian Native from Japan and Philippine and Latin-American Creole breeds was also included. Furthermore, the information from the IPD–MHC database was also used in the present analysis. DNA was genotyped using the sequence-based typing method. DNA electropherograms were analyzed using the Assign 400ATF software.Results: We detected 71 distinct alleles, including three new variants for the BoLA-DRB3 gene. Venn analysis showed that 11 of these alleles were only detected in Myanmar native breeds and 26 were only shared with Asian native and/or Zebu groups. The number of alleles ranged from 33 in Holstein-Friesians to 58 in Pyer Seins, and the observed versus unbiased expected heterozygosity were higher than 0.84 in all the three the populations analyzed. The FST analysis showed a low level of genetic differentiation between the two Myanmar native breeds (FST=0.003), and between these native breeds and the Holstein-Friesians (FST < 0.021). The average FST value for all the Myanmar Holstein-Friesian crossbred and Myanmar native populations was 0.0136 and 0.0121, respectively. Principal component analysis (PCA) and tree analysis showed that Myanmar native populations grouped in a narrow cluster that diverged clearly from the Holstein-Friesian populations. Furthermore, the BoLA-DRB3 allele frequencies suggested that while some Myanmar native populations from Bago, Mandalay and Yangon regions were more closely related to Zebu breeds (Gir and Brahman), populations from Kayin, Magway and Sagaing regions were more related to the Philippines native breeds. On the contrary, PCA showed that the Holstein-Friesian populations demonstrated a high degree of dispersion, which is likely the result of the different degrees of native admixture in these populations.Conclusion: This study is the first to report the genetic diversity of the BoLA-DRB3 gene in two native breeds and one exotic cattle crossbreed from Myanmar. The results obtained contribute to our understanding of the genetic diversity and distribution of BoLA-DRB3 gene alleles in Myanmar, and increases our knowledge of the worldwide variability of cattle BoLA-DRB3 genes, an important locus for immune response and protection against pathogens.

ACS Style

Guillermo Giovambattista; Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Si Thu Hein; Hla Hla Moe; Shin-Nosuke Takeshima; Yoko Aida. Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar. 2020, 1 .

AMA Style

Guillermo Giovambattista, Kyaw Kyaw Moe, Meripet Polat, Liushiqi Borjigin, Si Thu Hein, Hla Hla Moe, Shin-Nosuke Takeshima, Yoko Aida. Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar. . 2020; ():1.

Chicago/Turabian Style

Guillermo Giovambattista; Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Si Thu Hein; Hla Hla Moe; Shin-Nosuke Takeshima; Yoko Aida. 2020. "Characterization of bovine MHC DRB3 diversity in global cattle breeds, with a focus on cattle in Myanmar." , no. : 1.

Journal article
Published: 16 June 2020 in Viruses
Reads 0
Downloads 0

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle and is closely related to human T-cell leukemia viruses. We investigated the role of a new host protein, PRMT5, in BLV infection. We found that PRMT5 is overexpressed only in BLV-infected cattle with a high proviral load, but not in those with a low proviral load. Furthermore, this upregulation continued to the lymphoma stage. PRMT5 expression was upregulated in response to experimental BLV infection; moreover, PRMT5 upregulation began in an early stage of BLV infection rather than after a long period of proviral latency. Second, siRNA-mediated PRMT5 knockdown enhanced BLV gene expression at the transcript and protein levels. Additionally, a selective small-molecule inhibitor of PRMT5 (CMP5) enhanced BLV gene expression. Interestingly, CMP5 treatment, but not siRNA knockdown, altered the gp51 glycosylation pattern and increased the molecular weight of gp51, thereby decreasing BLV-induced syncytium formation. This was supported by the observation that CMP5 treatment enhanced the formation of the complex type of N-glycan more than the high mannose type. In conclusion, PRMT5 overexpression is related to the development of BLV infection with a high proviral load and lymphoma stage and PRMT5 inhibition enhances BLV gene expression. This is the first study to investigate the role of PRMT5 in BLV infection in vivo and in vitro and to reveal a novel function for a small-molecule compound in BLV-gp51 glycosylation processing.

ACS Style

Wlaa Assi; Tomoya Hirose; Satoshi Wada; Ryosuke Matsuura; Shin-Nosuke Takeshima; Yoko Aida. PRMT5 Is Required for Bovine Leukemia Virus Infection In Vivo and Regulates BLV Gene Expression, Syncytium Formation, and Glycosylation In Vitro. Viruses 2020, 12, 650 .

AMA Style

Wlaa Assi, Tomoya Hirose, Satoshi Wada, Ryosuke Matsuura, Shin-Nosuke Takeshima, Yoko Aida. PRMT5 Is Required for Bovine Leukemia Virus Infection In Vivo and Regulates BLV Gene Expression, Syncytium Formation, and Glycosylation In Vitro. Viruses. 2020; 12 (6):650.

Chicago/Turabian Style

Wlaa Assi; Tomoya Hirose; Satoshi Wada; Ryosuke Matsuura; Shin-Nosuke Takeshima; Yoko Aida. 2020. "PRMT5 Is Required for Bovine Leukemia Virus Infection In Vivo and Regulates BLV Gene Expression, Syncytium Formation, and Glycosylation In Vitro." Viruses 12, no. 6: 650.

Short report
Published: 22 April 2020 in Virology Journal
Reads 0
Downloads 0

Bovine leukemia virus (BLV) causes enzootic bovine leukosis, the most common neoplastic disease in cattle. We previously reported the development and protocol of the luminescence syncytium induction assay (LuSIA), a method for evaluating BLV infectivity based on CC81-GREMG cells. These cells form syncytia expressing enhanced green fluorescent protein when co-cultured with BLV-infected cells. Recently, we confirmed CAT1/SLC7A1 functions as a receptor of BLV. Here, we focused on CAT1/SLC7A1 to increase the sensitivity of LuSIA. We constructed a bovine CAT1-expressing plasmid and established a new CC81-GREMG-derived reporter cell line highly expressing bovine CAT1 (CC81-GREMG-CAT1). The new LuSIA protocol using CC81-GREMG-CAT1 cells measures cell-to-cell infectivity and cell-free infectivity of BLV faster and with greater sensitivity than the previous protocol using CC81-GREMG. The new LuSIA protocol is quantitative and more sensitive than the previous assay based on CC81-GREMG cells and will facilitate the development of several new BLV assays.

ACS Style

Hirotaka Sato; Lanlan Bai; Liushiqi Borjigin; Yoko Aida. Overexpression of bovine leukemia virus receptor SLC7A1/CAT1 enhances cellular susceptibility to BLV infection on luminescence syncytium induction assay (LuSIA). Virology Journal 2020, 17, 1 -6.

AMA Style

Hirotaka Sato, Lanlan Bai, Liushiqi Borjigin, Yoko Aida. Overexpression of bovine leukemia virus receptor SLC7A1/CAT1 enhances cellular susceptibility to BLV infection on luminescence syncytium induction assay (LuSIA). Virology Journal. 2020; 17 (1):1-6.

Chicago/Turabian Style

Hirotaka Sato; Lanlan Bai; Liushiqi Borjigin; Yoko Aida. 2020. "Overexpression of bovine leukemia virus receptor SLC7A1/CAT1 enhances cellular susceptibility to BLV infection on luminescence syncytium induction assay (LuSIA)." Virology Journal 17, no. 1: 1-6.

Journal article
Published: 23 March 2020 in Viruses
Reads 0
Downloads 0

Current therapies for human immunodeficiency virus type 1 (HIV-1) do not completely eliminate viral reservoirs in cells, such as macrophages. The HIV-1 accessory protein viral protein R (Vpr) promotes virus production in macrophages, and the maintenance of Vpr is essential for HIV-1 replication in these reservoir cells. We identified two novel Vpr-binding proteins, i.e., protein arginine N-methyltransferases (PRMTs) 5 and 7, using human monocyte-derived macrophages (MDMs). Both proteins found to be important for prevention of Vpr degradation by the proteasome; in the context of PRMT5 and PRMT7 knockdowns, degradation of Vpr could be prevented using a proteasome inhibitor. In MDMs infected with a wild-type strain, knockdown of PRMT5/PRMT7 and low expression of PRMT5 resulted in inefficient virus production like Vpr-deficient strain infections. Thus, our findings suggest that PRMT5 and PRMT7 support HIV-1 replication via maintenance of Vpr protein stability.

ACS Style

Hironobu Murakami; Takehiro Suzuki; Kiyoto Tsuchiya; Hiroyuki Gatanaga; Manabu Taura; Eriko Kudo; Seiji Okada; Masami Takei; Kazumichi Kuroda; Tatsuo Yamamoto; Kyoji Hagiwara; Naoshi Dohmae; Yoko Aida. Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production. Viruses 2020, 12, 355 .

AMA Style

Hironobu Murakami, Takehiro Suzuki, Kiyoto Tsuchiya, Hiroyuki Gatanaga, Manabu Taura, Eriko Kudo, Seiji Okada, Masami Takei, Kazumichi Kuroda, Tatsuo Yamamoto, Kyoji Hagiwara, Naoshi Dohmae, Yoko Aida. Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production. Viruses. 2020; 12 (3):355.

Chicago/Turabian Style

Hironobu Murakami; Takehiro Suzuki; Kiyoto Tsuchiya; Hiroyuki Gatanaga; Manabu Taura; Eriko Kudo; Seiji Okada; Masami Takei; Kazumichi Kuroda; Tatsuo Yamamoto; Kyoji Hagiwara; Naoshi Dohmae; Yoko Aida. 2020. "Protein Arginine N-methyltransferases 5 and 7 Promote HIV-1 Production." Viruses 12, no. 3: 355.

Journal article
Published: 22 March 2020 in Viruses
Reads 0
Downloads 0

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leucosis. However, less than 5% of BLV-infected cattle will develop lymphoma, suggesting that, in addition to viral infection, host genetic polymorphisms might play a role in disease susceptibility. Bovine leukocyte antigen (BoLA)-DRB3 is a highly polymorphic gene associated with BLV proviral load (PVL) susceptibility. Due to the fact that PVL is positively associated with disease progression, it is believed that controlling PVL can prevent lymphoma development. Thus, many studies have focused on the relationship between PVL and BoLA-DRB3. Despite this, there is little information regarding the relationship between lymphoma and BoLA-DRB3. Furthermore, whether or not PVL-associated BoLA-DRB3 is linked to lymphoma-associated BoLA-DRB3 has not been clarified. Here, we investigated whether or not lymphoma-associated BoLA-DRB3 is correlated with PVL-associated BoLA-DRB3. We demonstrate that two BoLA-DRB3 alleles were specifically associated with lymphoma resistance (*010:01 and *011:01), but no lymphoma-specific susceptibility alleles were found; furthermore, two other alleles, *002:01 and *012:01, were associated with PVL resistance and susceptibility, respectively. In contrast, lymphoma and PVL shared two resistance-associated (DRB3*014:01:01 and *009:02) BoLA-DRB3 alleles. Interestingly, we found that PVL associated alleles, but not lymphoma associated alleles, are related with the anti-BLV gp51 antibody production level in cows. Overall, our study is the first to demonstrate that the BoLA-DRB3 polymorphism confers differential susceptibility to BLV-induced lymphoma and PVL.

ACS Style

Chieh-Wen Lo; Liushiqi Borjigin; Susumu Saito; Koya Fukunaga; Etsuko Saitou; Katsunori Okazaki; Tetsuya Mizutani; Satoshi Wada; Shin-Nosuke Takeshima; Yoko Aida. BoLA-DRB3 Polymorphism is Associated with Differential Susceptibility to Bovine Leukemia Virus-Induced Lymphoma and Proviral Load. Viruses 2020, 12, 352 .

AMA Style

Chieh-Wen Lo, Liushiqi Borjigin, Susumu Saito, Koya Fukunaga, Etsuko Saitou, Katsunori Okazaki, Tetsuya Mizutani, Satoshi Wada, Shin-Nosuke Takeshima, Yoko Aida. BoLA-DRB3 Polymorphism is Associated with Differential Susceptibility to Bovine Leukemia Virus-Induced Lymphoma and Proviral Load. Viruses. 2020; 12 (3):352.

Chicago/Turabian Style

Chieh-Wen Lo; Liushiqi Borjigin; Susumu Saito; Koya Fukunaga; Etsuko Saitou; Katsunori Okazaki; Tetsuya Mizutani; Satoshi Wada; Shin-Nosuke Takeshima; Yoko Aida. 2020. "BoLA-DRB3 Polymorphism is Associated with Differential Susceptibility to Bovine Leukemia Virus-Induced Lymphoma and Proviral Load." Viruses 12, no. 3: 352.

Research article
Published: 21 February 2020 in PLOS ONE
Reads 0
Downloads 0

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, which is the most common neoplastic disease of cattle. BLV infects cattle worldwide and causes serious problems for the cattle industry. In this study, we examined the prevalence of BLV infection and the distribution of BLV genotypes in cattle in the northern, central, and southern parts of Myanmar. The prevalence of BLV infection among Myanmar cattle (37.04%) in this study was markedly higher than the prevalence (9.1%) observed in our earlier study in which BLV was detected from the limited number of cattle only from a small area of Myanmar. Phylogenetic analysis of partial env-gp51 sequence of the isolated BLV strains revealed that there are at least three BLV genotypes (genotype-1, genotype-6, and genotype-10) in Myanmar, which have also been detected in the neighboring countries. We performed this study to estimate the BLV proviral load, which is a major diagnosis index for determining the virus transmission risk. The cattle of the three test regions with warm, wet, and humid climatic conditions (upper Sagaing, Yangon, and Kayin) exhibited a high mean proviral load, while cattle of three other regions with low annual rainfall and very high temperature (Mandalay, Magway, and upper Bago) exhibited a low mean proviral load. Further, the level of proviral load and the prevalence of BLV infection in Myanmar native cattle (N = 235) were lower than that in the hybrid cattle (Holstein Friesian × Myanmar native) (N = 62). We also observed that the cattle with high risk for BLV transmission, which have high proviral load, may enhance the BLV infection rate. Hence, to control BLV transmission, it is necessary to eliminate these cattle with high-risk for BLV transmission and to diagnose BLV provirus in cattle in the remaining regions/states of Myanmar sharing a boundary with neighboring countries.

ACS Style

Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Ryosuke Matsuura; Si Thu Hein; Hla Hla Moe; Yoko Aida. New evidence of bovine leukemia virus circulating in Myanmar cattle through epidemiological and molecular characterization. PLOS ONE 2020, 15, e0229126 .

AMA Style

Kyaw Kyaw Moe, Meripet Polat, Liushiqi Borjigin, Ryosuke Matsuura, Si Thu Hein, Hla Hla Moe, Yoko Aida. New evidence of bovine leukemia virus circulating in Myanmar cattle through epidemiological and molecular characterization. PLOS ONE. 2020; 15 (2):e0229126.

Chicago/Turabian Style

Kyaw Kyaw Moe; Meripet Polat; Liushiqi Borjigin; Ryosuke Matsuura; Si Thu Hein; Hla Hla Moe; Yoko Aida. 2020. "New evidence of bovine leukemia virus circulating in Myanmar cattle through epidemiological and molecular characterization." PLOS ONE 15, no. 2: e0229126.

Journal article
Published: 15 January 2020 in Viruses
Reads 0
Downloads 0

Viral protein R (Vpr) is an accessory protein found in various primate lentiviruses, including human immunodeficiency viruses type 1 and 2 (HIV-1 and HIV-2) as well as simian immunodeficiency viruses (SIVs). Vpr modulates many processes during viral lifecycle via interaction with several of cellular targets. Previous studies showed that HIV-1 Vpr strengthened degradation of Mini-chromosome Maintenance Protein10 (MCM10) by manipulating DCAF1-Cul4-E3 ligase in proteasome-dependent pathway. However, whether Vpr from other primate lentiviruses are also associated with MCM10 degradation and the ensuing impact remain unknown. Based on phylogenetic analyses, a panel of primate lentiviruses Vpr/x covering main virus lineages was prepared. Distinct MCM10 degradation profiles were mapped and HIV-1, SIVmus and SIVrcm Vprs induced MCM10 degradation in proteasome-dependent pathway. Colocalization and interaction between MCM10 with these Vprs were also observed. Moreover, MCM10 2-7 interaction region was identified as a determinant region susceptible to degradation. However, MCM10 degradation did not alleviate DNA damage response induced by these Vpr proteins. MCM10 degradation by HIV-1 Vpr proteins was correlated with G2/M arrest, while induction of apoptosis and oligomerization formation of Vpr failed to alter MCM10 proteolysis. The current study demonstrated a distinct interplay pattern between primate lentiviruses Vpr proteins and MCM10.

ACS Style

Hao Chang; Lowela Siarot; Ryosuke Matsuura; Chieh-Wen Lo; Hirotaka Sato; Hiroyuki Otsuki; Yoko Aida. Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins. Viruses 2020, 12, 98 .

AMA Style

Hao Chang, Lowela Siarot, Ryosuke Matsuura, Chieh-Wen Lo, Hirotaka Sato, Hiroyuki Otsuki, Yoko Aida. Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins. Viruses. 2020; 12 (1):98.

Chicago/Turabian Style

Hao Chang; Lowela Siarot; Ryosuke Matsuura; Chieh-Wen Lo; Hirotaka Sato; Hiroyuki Otsuki; Yoko Aida. 2020. "Distinct MCM10 Proteasomal Degradation Profiles by Primate Lentiviruses Vpr Proteins." Viruses 12, no. 1: 98.

Journal article
Published: 01 January 2020 in Journal of Veterinary Medical Science
Reads 0
Downloads 0

Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis, the most common neoplastic disease of cattle worldwide and a serious problem for the cattle industry. Previous studies have shown the molecular prevalence of BLV and the coexistence of BLV genotype-1 and -4 in Egyptian dairy cattle; however, the molecular characteristics of BLV in Egyptian beef cattle are unknown. Therefore, we collected blood samples of 168 beef cattle from slaughterhouses in three governorates in Egypt. Based on BLV-CoCoMo-qPCR-2 targeting long terminal repeats and nested PCR targeting the env-gp51 gene, the BLV provirus infection rates were found to be 47/168 (28.0%) and 42/168 (25.0%), respectively. Phylogenetic analysis based on 501 bp of the BLV env-gp51 gene from 42 BLV isolates revealed that at least six distinctive strains (b, e, f, g, x, and z) were prevalent in cattle across the examined regions. Furthermore, phylogenetic analysis of the 420 bp sequence of the BLV env-gp51 region of the six strains against 11 known genotypes showed that the strains b, e, f, and g were clustered into genotype-1, and strains x and z were clustered into genotype-4. Our results also indicated that strains b and x exist in both dairy and beef cattle in Egypt. The present study is the first to detect and genotype BLV among beef cattle in Egypt.

ACS Style

Samy Metwally; Rania Hamada; Alsagher O. Ali; Hassan Y.A.H. Mahmoud; Nabil M. Baker; Adel E. A. Mohamed; Satoshi Wada; Yasunobu Matsumoto; Yoko Aida. Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt. Journal of Veterinary Medical Science 2020, 82, 20-0477 -1684.

AMA Style

Samy Metwally, Rania Hamada, Alsagher O. Ali, Hassan Y.A.H. Mahmoud, Nabil M. Baker, Adel E. A. Mohamed, Satoshi Wada, Yasunobu Matsumoto, Yoko Aida. Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt. Journal of Veterinary Medical Science. 2020; 82 (11):20-0477-1684.

Chicago/Turabian Style

Samy Metwally; Rania Hamada; Alsagher O. Ali; Hassan Y.A.H. Mahmoud; Nabil M. Baker; Adel E. A. Mohamed; Satoshi Wada; Yasunobu Matsumoto; Yoko Aida. 2020. "Detection and molecular characterization of bovine leukemia virus in beef cattle presented for slaughter in Egypt." Journal of Veterinary Medical Science 82, no. 11: 20-0477-1684.

Short report
Published: 16 December 2019 in Virology Journal
Reads 0
Downloads 0

Bovine leukemia virus (BLV), which is closely related to human T-cell leukemia virus, is the etiological agent of enzootic bovine leukosis, a disease characterized by a highly prolonged course involving persistent lymphocytosis and B-cell lymphoma. The bovine major histocompatibility complex class II region plays a key role in the subclinical progression of BLV infection. In this study, we aimed to evaluate the roles of CD4+ T-cell epitopes in disease progression in cattle. We examined five Japanese Black cattle, including three disease-susceptible animals, one disease-resistant animal, and one normal animal, classified according to genotyping of bovine leukocyte antigen (BoLA)-DRB3 and BoLA-DQA1 alleles using polymerase chain reaction sequence-based typing methods. All cattle were inoculated with BLV-infected blood collected from BLV experimentally infected cattle and then subjected to CD4+ T-cell epitope mapping by cell proliferation assays. Five Japanese Black cattle were successfully infected with BLV, and CD4+ T-cell epitope mapping was then conducted. Disease-resistant and normal cattle showed low and moderate proviral loads and harbored six or five types of CD4+ T-cell epitopes, respectively. In contrast, the one of three disease-susceptible cattle with the highest proviral load did not harbor CD4+ T-cell epitopes, and two of three other cattle with high proviral loads each had only one epitope. Thus, the CD4+ T-cell epitope repertoire was less frequent in disease-susceptible cattle than in other cattle. Although only a few cattle were included in this study, our results showed that CD4+ T-cell epitopes may be associated with BoLA-DRB3-DQA1 haplotypes, which conferred differential susceptibilities to BLV proviral loads. These CD4+ T-cell epitopes could be useful for the design of anti-BLV vaccines targeting disease-susceptible Japanese Black cattle. Further studies of CD4+ T-cell epitopes in other breeds and using larger numbers of cattle with differential susceptibilities are required to confirm these findings.

ACS Style

Lanlan Bai; Shin-Nosuke Takeshima; Masaaki Sato; William C. Davis; Satoshi Wada; Junko Kohara; Yoko Aida. Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression. Virology Journal 2019, 16, 1 -8.

AMA Style

Lanlan Bai, Shin-Nosuke Takeshima, Masaaki Sato, William C. Davis, Satoshi Wada, Junko Kohara, Yoko Aida. Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression. Virology Journal. 2019; 16 (1):1-8.

Chicago/Turabian Style

Lanlan Bai; Shin-Nosuke Takeshima; Masaaki Sato; William C. Davis; Satoshi Wada; Junko Kohara; Yoko Aida. 2019. "Mapping of CD4+ T-cell epitopes in bovine leukemia virus from five cattle with differential susceptibilities to bovine leukemia virus disease progression." Virology Journal 16, no. 1: 1-8.