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Seungki Lee
Biological and Genetic Resources Assessment Division, National Institute of Biological Resources, Incheon, 22689, Republic of Korea

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Short communication
Published: 16 December 2020 in Biochemical and Biophysical Research Communications
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No effective cryopreservation technique exists for fish eggs and embryos; thus, the cryopreservation of germ cells (spermatogonia or oogonia) and subsequent generation of eggs and sperm would be an alternative solution for the long-term preservation of piscine genetic resources. Nevertheless, in our previous study using rainbow trout, we showed that recipients transplanted with XY spermatogonia or XX oogonia produced unnatural sex-biased F1 offspring. To overcome these obstacles, we transplanted immature germ cells (XX oogonia or XY spermatogonia; frozen for 33 days) into the body cavities of triploid hatchlings, and the transplanted germ cells possessed a high capacity for differentiating into eggs and sperm in the ovaries and testes of recipients. Approximately 30% of triploid recipients receiving frozen germ cells generated normal salmon that displayed the donor-derived black body color phenotype, although all triploid salmon not receiving transplants were functionally sterile. Furthermore, F1 offspring obtained from insemination of the oogonia-derived eggs and spermatogonia-derived sperm show a normal sex ratio of 1:1 (female:male). Thus, this method presented a critical technique for practical conservation projects for other teleost fish species and masu salmon.

ACS Style

Seungki Lee; Woo Young Bang; Hee-Sun Yang; Dae-Sung Lee; Ha Yeun Song. Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells. Biochemical and Biophysical Research Communications 2020, 535, 6 -11.

AMA Style

Seungki Lee, Woo Young Bang, Hee-Sun Yang, Dae-Sung Lee, Ha Yeun Song. Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells. Biochemical and Biophysical Research Communications. 2020; 535 ():6-11.

Chicago/Turabian Style

Seungki Lee; Woo Young Bang; Hee-Sun Yang; Dae-Sung Lee; Ha Yeun Song. 2020. "Production of juvenile masu salmon (Oncorhynchus masou) from spermatogonia-derived sperm and oogonia-derived eggs via intraperitoneal transplantation of immature germ cells." Biochemical and Biophysical Research Communications 535, no. : 6-11.

Journal article
Published: 19 July 2020 in Antibiotics
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The prevalence of antibiotic-resistant bacteria has become an immediate threat to public health. Antimicrobial peptides are attracting attention as a new source of antibiotics due to their ability to prevent drug-resistances with fewer side effects. Spider venom is composed of various bioactive substances with multiple functionalities such as antimicrobial and anti-inflammatory effects. Here, RNA sequencing was conducted on the venom gland of the spider Pardosa astrigera, and a potential toxin peptide with antibacterial properties was selected via homology and in silico analysis. A novel toxin, Lycotoxin-Pa4a, inhibited both gram-negative and gram-positive bacteria by disrupting the outer and bacterial cytoplasmic membrane. Moreover, the peptide downregulated the expression of pro-inflammatory mediators while upregulating the level of anti-inflammatory cytokine by inactivating mitogen-activated protein kinase signaling in a lipopolysaccharide-stimulated murine macrophage cell line. In this research, we identified a novel peptide toxin, Lycotoxin-pa4a, with antibacterial and anti-inflammatory properties, suggesting its potential for the development of a new antibiotics, as well as offering insights into the utilization of biological resources.

ACS Style

Min Shin; In-Wook Hwang; YunKyung Kim; Seung Kim; Wonhee Jang; Seungki Lee; Woo Bang; Chang-Hwan Bae; Jung-Suk Sung. Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera. Antibiotics 2020, 9, 422 .

AMA Style

Min Shin, In-Wook Hwang, YunKyung Kim, Seung Kim, Wonhee Jang, Seungki Lee, Woo Bang, Chang-Hwan Bae, Jung-Suk Sung. Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera. Antibiotics. 2020; 9 (7):422.

Chicago/Turabian Style

Min Shin; In-Wook Hwang; YunKyung Kim; Seung Kim; Wonhee Jang; Seungki Lee; Woo Bang; Chang-Hwan Bae; Jung-Suk Sung. 2020. "Antibacterial and Anti-Inflammatory Effects of Novel Peptide Toxin from the Spider Pardosa astrigera." Antibiotics 9, no. 7: 422.

Journal article
Published: 17 July 2020 in Microorganisms
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Phenol and formalin are major water pollutants that are frequently discharged into the aquatic milieu. These chemicals can affect broad domains of life, including microorganisms. Aquatic pollutants, unlike terrestrial pollutants, are easily diluted in water environments and exist at a sub-inhibitory concentration (sub-IC), thus not directly inhibiting bacterial growth. However, they can modulate gene expression profiles. The sub-IC values of phenol and formalin were measured by minimal inhibitory concentration (MIC) assay to be 0.146% (1.3 mM) and 0.0039% (0.38 mM), respectively, in Edwardsiella piscicida CK108, a Gram-negative fish pathogen. We investigated the differentially expressed genes (DEG) by RNA-seq when the cells were exposed to the sub-ICs of phenol and formalin. DEG analyses revealed that genes involved in major virulence factors (type I fimbriae, flagella, type III and type VI secretion system) and various cellular pathways (energy production, amino acid synthesis, carbohydrate metabolism and two-component regulatory systems) were up- or downregulated by both chemicals. The genome-wide gene expression data corresponded to the results of a quantitative reverse complementary-PCR and motility assay. This study not only provides insight into how a representative fish pathogen, E. piscicida CK108, responds to the sub-ICs of phenol and formalin but also shows the importance of controlling chemical pollutants in aquatic environments.

ACS Style

Ju Bin Yoon; Sungmin Hwang; Se-Won Baek; Seungki Lee; Woo Young Bang; Ki Hwan Moon. In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms. Microorganisms 2020, 8, 1068 .

AMA Style

Ju Bin Yoon, Sungmin Hwang, Se-Won Baek, Seungki Lee, Woo Young Bang, Ki Hwan Moon. In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms. Microorganisms. 2020; 8 (7):1068.

Chicago/Turabian Style

Ju Bin Yoon; Sungmin Hwang; Se-Won Baek; Seungki Lee; Woo Young Bang; Ki Hwan Moon. 2020. "In vitro Edwardsiella piscicida CK108 Transcriptome Profiles with Subinhibitory Concentrations of Phenol and Formalin Reveal New Insights into Bacterial Pathogenesis Mechanisms." Microorganisms 8, no. 7: 1068.

Journal article
Published: 01 July 2020 in Toxins
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Bacteriocins are functionally diverse toxins produced by most microbes and are potent antimicrobial peptides (AMPs) for bacterial ghosts as next generation vaccines. Here, we first report that the AMPs secreted from Lactobacillus taiwanensis effectively form ghosts of pathogenic bacteria and are identified as diverse bacteriocins, including novel ones. In detail, a cell-free supernatant from L. taiwanensis exhibited antimicrobial activities against pathogenic bacteria and was observed to effectively cause cellular lysis through pore formation in the bacterial membrane using scanning electron microscopy (SEM). The treatment of the cell-free supernatant with proteinase K or EDTA proved that the antimicrobial activity is mediated by AMPs, and the purification of AMPs using Sep-Pak columns indicated that the cell-free supernatant includes various amphipathic peptides responsible for the antimicrobial activity. Furthermore, the whole-genome sequencing of L. taiwanensis revealed that the strain has diverse bacteriocins, confirmed experimentally to function as AMPs, and among them are three novel bacteriocins, designated as Tan 1, Tan 2, and Tan 3. We also confirmed, using SEM, that Tan 2 effectively produces bacterial ghosts. Therefore, our data suggest that the bacteriocins from L. taiwanensis are potentially useful as a critical component for the preparation of bacterial ghosts.

ACS Style

Sam Kim; Yeon Ha; Kyu Bang; Seungki Lee; Joo-Hong Yeo; Hee-Sun Yang; Tae-Won Kim; Kyu Lee; Woo Bang. Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine. Toxins 2020, 12, 432 .

AMA Style

Sam Kim, Yeon Ha, Kyu Bang, Seungki Lee, Joo-Hong Yeo, Hee-Sun Yang, Tae-Won Kim, Kyu Lee, Woo Bang. Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine. Toxins. 2020; 12 (7):432.

Chicago/Turabian Style

Sam Kim; Yeon Ha; Kyu Bang; Seungki Lee; Joo-Hong Yeo; Hee-Sun Yang; Tae-Won Kim; Kyu Lee; Woo Bang. 2020. "Potential of Bacteriocins from Lactobacillus taiwanensis for Producing Bacterial Ghosts as a Next Generation Vaccine." Toxins 12, no. 7: 432.

Mitogenome announcement
Published: 28 May 2020 in Mitochondrial DNA Part B
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The complete mitochondrial genome of the white grouper Epinephelus aeneus, which belongs to the family Serranidae, was determined. The complete mitochondrial genome measured 16,578 bp in length and consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. The mitochondrial gene arrangement of E. aeneus was typical of vertebrates. Phylogenetic analysis conducted using the mitochondrial genomes of 13 related species showed that E. aeneus clustered with other Serranidae species. This mitochondrial genome provides an important resource for addressing taxonomic issues and developing conservation strategies.

ACS Style

Seungki Lee; Dae-Sung Lee; Jong Su Yoo; Ha Yeun Song. Characterization of the complete mitochondrial genome of the white grouper Epinephelus aeneus (Perciformes, Serranidae) and a comparative analysis with other Serranidae species. Mitochondrial DNA Part B 2020, 5, 2226 -2227.

AMA Style

Seungki Lee, Dae-Sung Lee, Jong Su Yoo, Ha Yeun Song. Characterization of the complete mitochondrial genome of the white grouper Epinephelus aeneus (Perciformes, Serranidae) and a comparative analysis with other Serranidae species. Mitochondrial DNA Part B. 2020; 5 (3):2226-2227.

Chicago/Turabian Style

Seungki Lee; Dae-Sung Lee; Jong Su Yoo; Ha Yeun Song. 2020. "Characterization of the complete mitochondrial genome of the white grouper Epinephelus aeneus (Perciformes, Serranidae) and a comparative analysis with other Serranidae species." Mitochondrial DNA Part B 5, no. 3: 2226-2227.

Mitogenome announcement
Published: 12 May 2020 in Mitochondrial DNA Part B
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The complete mitochondrial genome of the Oriental reed warbler, Acrocephalus orientalis, which belongs to the family Sylviidae was determined. The complete mitochondrial genome has a length of 16,971 bp and consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. A. orientalis has a mitochondrial gene arrangement that is typical of vertebrates. Phylogenetic analysis using mitochondrial genomes of 11 related species revealed that A. orientalis is clustered with Acrocephalus scirpaceus and rooted with the other Sylviidae species. This mitochondrial genome provides an important resource for addressing taxonomic issues and studying molecular evolution.

ACS Style

Sung Hyun Kim; Soon Kyoo Choi; Seungki Lee. Characterization of the complete mitochondrial genome of Oriental reed warbler, Acrocephalus orientalis (Aves: Passeriformes), and a comparative analysis with other Sylviidae species. Mitochondrial DNA Part B 2020, 5, 2028 -2029.

AMA Style

Sung Hyun Kim, Soon Kyoo Choi, Seungki Lee. Characterization of the complete mitochondrial genome of Oriental reed warbler, Acrocephalus orientalis (Aves: Passeriformes), and a comparative analysis with other Sylviidae species. Mitochondrial DNA Part B. 2020; 5 (3):2028-2029.

Chicago/Turabian Style

Sung Hyun Kim; Soon Kyoo Choi; Seungki Lee. 2020. "Characterization of the complete mitochondrial genome of Oriental reed warbler, Acrocephalus orientalis (Aves: Passeriformes), and a comparative analysis with other Sylviidae species." Mitochondrial DNA Part B 5, no. 3: 2028-2029.

Journal article
Published: 30 July 2019 in Microorganisms
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The emergence and spread of antibiotic-resistant Aeromonas spp. is a serious public and animal health concern. Wild animals serve as reservoirs, vectors, and sentinels of these bacteria and can facilitate their transmission to humans and livestock. The nutria (Myocastor coypus), a semi-aquatic rodent, currently is globally considered an invasive alien species that has harmful impacts on natural ecosystems and carries various zoonotic aquatic pathogens. This study aimed to determine the prevalence of antibiotic-resistant zoonotic Aeromonas spp. in wild invasive nutrias captured in Korea during governmental eradication program. Three potential zoonotic Aeromonas spp. (A. hydrophila, A. caviae, and A. dhakensis) were identified among isolates from nutria. Some strains showed unexpected resistance to fluoroquinolones, third-generation cephalosporins, and carbapenems. In carbapenem-resistant isolates, the cphA gene, which is related to intrinsic resistance of Aeromonas to carbapenems, was identified, and phylogenetic analysis based on this gene revealed the presence of two major groups represented by A. hydrophila (including A. dhakensis) and other Aeromonas spp. These results indicate that wild nutrias in Korea are a potential reservoir of zoonotic and antibiotic-resistant Aeromonas spp. that can cause infection and treatment failure in humans. Thus, measures to prevent contact of wild nutrias with livestock and humans are needed.

ACS Style

Se Ra Lim; Do-Hun Lee; Seon Young Park; Seungki Lee; Hyo Yeon Kim; Moo-Seung Lee; Jung Ro Lee; Jee Eun Han; Hye Kwon Kim; Ji Hyung Kim. Wild Nutria (Myocastor coypus) Is a Potential Reservoir of Carbapenem-Resistant and Zoonotic Aeromonas spp. in Korea. Microorganisms 2019, 7, 224 .

AMA Style

Se Ra Lim, Do-Hun Lee, Seon Young Park, Seungki Lee, Hyo Yeon Kim, Moo-Seung Lee, Jung Ro Lee, Jee Eun Han, Hye Kwon Kim, Ji Hyung Kim. Wild Nutria (Myocastor coypus) Is a Potential Reservoir of Carbapenem-Resistant and Zoonotic Aeromonas spp. in Korea. Microorganisms. 2019; 7 (8):224.

Chicago/Turabian Style

Se Ra Lim; Do-Hun Lee; Seon Young Park; Seungki Lee; Hyo Yeon Kim; Moo-Seung Lee; Jung Ro Lee; Jee Eun Han; Hye Kwon Kim; Ji Hyung Kim. 2019. "Wild Nutria (Myocastor coypus) Is a Potential Reservoir of Carbapenem-Resistant and Zoonotic Aeromonas spp. in Korea." Microorganisms 7, no. 8: 224.

Original research article
Published: 10 September 2018 in Frontiers in Microbiology
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Microalgae are considered as sustainable resources for biofuel production. However, recently the focus on microalgal research has shifted toward the investigation of high-value metabolites for potential pharmaceutical and nutritional applications. Herein, we report the identification of a novel oleaginous green microalga isolated from the Yellow Sea in Korea. We also describe the morphological, molecular, and biochemical characteristics of this microalga. On the basis of microscopic and genetic analyses, the isolate was classified as Lobosphaera incisa (the strain was designated as K-1), and molecular phylogeny revealed that the isolate distinctly differed from the other known L. incisa strains. The microalga could be cultivated in various commercial culture media under a relatively broad range of pH and temperature conditions. We also did a rough and detailed estimation of the different cellular components in the microalga. The composition of arachidonic acid (C20:4ω6) in the lipids of L. incisa strain K-1 was relatively high, similar to that in other strains, however, the K-1 strain had higher proportions of the ω3 series of fatty acids (FAs), including α-linolenic acid (C18:3ω3) and eicosapentaenoic acid (C20:5ω3), highlighting its uniqueness and strong potential for biotechnological application. To the best of our knowledge, this is the first report on the isolation of L. incisa from Korea as well as from a marine environment; this novel strain might be useful for the production of high-value ω3 and ω6 polyunsaturated fatty acids (PUFAs).

ACS Style

Seungki Lee; Se Ra Lim; Dae Gwin Jeong; Ji Hyung Kim. Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea. Frontiers in Microbiology 2018, 9, 2159 .

AMA Style

Seungki Lee, Se Ra Lim, Dae Gwin Jeong, Ji Hyung Kim. Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea. Frontiers in Microbiology. 2018; 9 ():2159.

Chicago/Turabian Style

Seungki Lee; Se Ra Lim; Dae Gwin Jeong; Ji Hyung Kim. 2018. "Characterization of an Oleaginous Unicellular Green Microalga, Lobosphaera incisa (Reisigl, 1964) Strain K-1, Isolated From a Tidal Flat in the Yellow Sea, Republic of Korea." Frontiers in Microbiology 9, no. : 2159.

Journal article
Published: 01 June 2018 in Pesticide Biochemistry and Physiology
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ACS Style

Loan Thi Thanh Nguyen; Ja Yeong Jang; Tae Yoon Kim; Nan Hee Yu; Ae Ran Park; Seungki Lee; Chang-Hwan Bae; Joo Hong Yeo; Jae-Seoun Hur; Hae Woong Park; Jin-Cheol Kim. Nematicidal activity of verrucarin A and roridin A isolated from Myrothecium verrucaria against Meloidogyne incognita. Pesticide Biochemistry and Physiology 2018, 148, 133 -143.

AMA Style

Loan Thi Thanh Nguyen, Ja Yeong Jang, Tae Yoon Kim, Nan Hee Yu, Ae Ran Park, Seungki Lee, Chang-Hwan Bae, Joo Hong Yeo, Jae-Seoun Hur, Hae Woong Park, Jin-Cheol Kim. Nematicidal activity of verrucarin A and roridin A isolated from Myrothecium verrucaria against Meloidogyne incognita. Pesticide Biochemistry and Physiology. 2018; 148 ():133-143.

Chicago/Turabian Style

Loan Thi Thanh Nguyen; Ja Yeong Jang; Tae Yoon Kim; Nan Hee Yu; Ae Ran Park; Seungki Lee; Chang-Hwan Bae; Joo Hong Yeo; Jae-Seoun Hur; Hae Woong Park; Jin-Cheol Kim. 2018. "Nematicidal activity of verrucarin A and roridin A isolated from Myrothecium verrucaria against Meloidogyne incognita." Pesticide Biochemistry and Physiology 148, no. : 133-143.

Journal article
Published: 03 April 2018 in Stem Cell Research
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The conservation of endangered fish is an urgent issue. Although cryo-banking of fish gametes might ultimately help conserve endangered fish, cryopreservation of fish eggs or embryos is still not possible due to their large size and high yolk content. Therefore, as an alternative, we focused on undifferentiated germ cells, such as primordial germ cells, spermatogonia, and oogonia, as materials for cryopreservation. Transplantation of cryopreserved germ cells into the body cavity of allogeneic or xenogeneic recipients sterilized by triploidization or endogenous germ cell ablation resulted in migration of the transplanted germ cells toward the recipient genital ridges, where they were eventually incorporated. The donor-derived germ cells initiated either spermatogenesis or oogenesis in the recipient gonads, depending on the sex of the recipient. Furthermore, by mating the male and female recipients, viable offspring derived from the frozen germ cells were produced. Although this technology was established using salmonid fish, we found that it is applicable to a wide range of fish species. Thus, this method might represent a “silver bullet” for preserving the valuable genetic resources of endangered fish species.

ACS Style

Goro Yoshizaki; Seungki Lee. Production of live fish derived from frozen germ cells via germ cell transplantation. Stem Cell Research 2018, 29, 103 -110.

AMA Style

Goro Yoshizaki, Seungki Lee. Production of live fish derived from frozen germ cells via germ cell transplantation. Stem Cell Research. 2018; 29 ():103-110.

Chicago/Turabian Style

Goro Yoshizaki; Seungki Lee. 2018. "Production of live fish derived from frozen germ cells via germ cell transplantation." Stem Cell Research 29, no. : 103-110.

Journal article
Published: 01 December 2017 in Journal of Global Antimicrobial Resistance
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ACS Style

Jee Eun Han; Seungki Lee; Dae Gwin Jeong; Sun-Woo Yoon; O-Jin Kim; Moo-Seung Lee; Hye Kwon Kim; Sung-Kyun Park; Ji Hyung Kim; Se Chang Park. Complete genome sequence of multidrug-resistant Staphylococcus sciuri strain SNUDS-18 isolated from a farmed duck in South Korea. Journal of Global Antimicrobial Resistance 2017, 11, 108 -110.

AMA Style

Jee Eun Han, Seungki Lee, Dae Gwin Jeong, Sun-Woo Yoon, O-Jin Kim, Moo-Seung Lee, Hye Kwon Kim, Sung-Kyun Park, Ji Hyung Kim, Se Chang Park. Complete genome sequence of multidrug-resistant Staphylococcus sciuri strain SNUDS-18 isolated from a farmed duck in South Korea. Journal of Global Antimicrobial Resistance. 2017; 11 ():108-110.

Chicago/Turabian Style

Jee Eun Han; Seungki Lee; Dae Gwin Jeong; Sun-Woo Yoon; O-Jin Kim; Moo-Seung Lee; Hye Kwon Kim; Sung-Kyun Park; Ji Hyung Kim; Se Chang Park. 2017. "Complete genome sequence of multidrug-resistant Staphylococcus sciuri strain SNUDS-18 isolated from a farmed duck in South Korea." Journal of Global Antimicrobial Resistance 11, no. : 108-110.

Research article
Published: 13 October 2017 in Journal of Applied Toxicology
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An evaluation of intestinal toxicity is important because the mucosal lining of the gastrointestinal tract is the first barrier for oral xenobiotics. Until now, a rat model has been recommended as the standard intestinal toxicity model and the Caco‐2 cell line, originated from a human colon adenocarcinoma, has been used as an alternative to this model, but there are limitations regarding cost‐effectiveness and the need for mimicry of the human system. In this study, we investigated whether zebrafish could be a valid alternative to rats and Caco‐2 cells as an intestinal toxicity model. We focused on intestinal gene expression of cytochrome P450 3A65, oxidative stress, apoptosis, inflammation, and intestinal function. Reverse transcription–quantitative polymerase chain reaction analysis was conducted using three models: zebrafish, Sprague–Dawley rats and Caco‐2 cells, and the transcript levels and patterns of indicator genes were analyzed in conjunction with histopathological changes. Our results suggested that representative intestinal toxicants, indomethacin, diclofenac and methotrexate, induced significant transcript level changes in marker genes such as CYP3A, inducible nitric oxide synthase, heme oxygenase 1, superoxide dismutase 1, glutathione peroxidase 1, BCL2 associated X, B‐cell lymphoma 2, caspase 9, tumor protein p53, nuclear factor‐κB, interleukin‐1β, tumor necrosis factor‐alphaα and toll‐like receptor 2 in the zebrafish model as in the rat and Caco‐2 cells models. These results suggest that zebrafish model is sufficiently worth developing as an intestinal toxicity model that can replace or compensate the rat model or Caco‐2 cell model.

ACS Style

Bokyeong Ryu; C-Yoon Kim; Hanseul Oh; Ukjin Kim; Jin Kim; Cho-Rok Jung; Byoung-Hee Lee; Seungki Lee; Seo-Na Chang; Ji Min Lee; Hyung-Min Chung; Jae-Hak Park. Development of an alternative zebrafish model for drug-induced intestinal toxicity. Journal of Applied Toxicology 2017, 38, 259 -273.

AMA Style

Bokyeong Ryu, C-Yoon Kim, Hanseul Oh, Ukjin Kim, Jin Kim, Cho-Rok Jung, Byoung-Hee Lee, Seungki Lee, Seo-Na Chang, Ji Min Lee, Hyung-Min Chung, Jae-Hak Park. Development of an alternative zebrafish model for drug-induced intestinal toxicity. Journal of Applied Toxicology. 2017; 38 (2):259-273.

Chicago/Turabian Style

Bokyeong Ryu; C-Yoon Kim; Hanseul Oh; Ukjin Kim; Jin Kim; Cho-Rok Jung; Byoung-Hee Lee; Seungki Lee; Seo-Na Chang; Ji Min Lee; Hyung-Min Chung; Jae-Hak Park. 2017. "Development of an alternative zebrafish model for drug-induced intestinal toxicity." Journal of Applied Toxicology 38, no. 2: 259-273.

Journal article
Published: 01 September 2017 in Journal of Global Antimicrobial Resistance
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ACS Style

Jee Eun Han; Seungki Lee; Dae Gwin Jeong; Sun-Woo Yoon; O-Jin Kim; Moo-Seung Lee; Hye Kwon Kim; Sung-Kyun Park; Ji Hyung Kim; Se Chang Park. Complete genome sequence of multidrug-resistant Staphylococcus cohnii ssp. urealyticus strain SNUDS-2 isolated from farmed duck, Republic of Korea. Journal of Global Antimicrobial Resistance 2017, 10, 37 -39.

AMA Style

Jee Eun Han, Seungki Lee, Dae Gwin Jeong, Sun-Woo Yoon, O-Jin Kim, Moo-Seung Lee, Hye Kwon Kim, Sung-Kyun Park, Ji Hyung Kim, Se Chang Park. Complete genome sequence of multidrug-resistant Staphylococcus cohnii ssp. urealyticus strain SNUDS-2 isolated from farmed duck, Republic of Korea. Journal of Global Antimicrobial Resistance. 2017; 10 ():37-39.

Chicago/Turabian Style

Jee Eun Han; Seungki Lee; Dae Gwin Jeong; Sun-Woo Yoon; O-Jin Kim; Moo-Seung Lee; Hye Kwon Kim; Sung-Kyun Park; Ji Hyung Kim; Se Chang Park. 2017. "Complete genome sequence of multidrug-resistant Staphylococcus cohnii ssp. urealyticus strain SNUDS-2 isolated from farmed duck, Republic of Korea." Journal of Global Antimicrobial Resistance 10, no. : 37-39.

Journal article
Published: 03 March 2017 in Scientific Reports
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The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification. After thawing, the cells dissociated from cryopreserved testicular tissues were intraperitoneally transplanted into sterile triploid hatchlings. Some cells, presumably spermatogonial stem cells, migrated into the genital ridges of recipients and resulted in the production of eggs or sperm, based on sex of the recipient. Mating of recipients resulted in successful production of cryopreserved TC-derived offspring. We successfully produced individuals from the Kaga inbred line, an endangered wild population in Tokyo, and a sub-fertile mutant (wnt4b−/−) from cryopreserved their TCs. This methodology facilitates semi-permanent preservation of various medaka strains.

ACS Style

Shinsuke Seki; Kazunari Kusano; Seungki Lee; Yoshiko Iwasaki; Masaru Yagisawa; Mariko Ishida; Tadashi Hiratsuka; Takao Sasado; Kiyoshi Naruse; Goro Yoshizaki. Production of the medaka derived from vitrified whole testes by germ cell transplantation. Scientific Reports 2017, 7, srep43185 .

AMA Style

Shinsuke Seki, Kazunari Kusano, Seungki Lee, Yoshiko Iwasaki, Masaru Yagisawa, Mariko Ishida, Tadashi Hiratsuka, Takao Sasado, Kiyoshi Naruse, Goro Yoshizaki. Production of the medaka derived from vitrified whole testes by germ cell transplantation. Scientific Reports. 2017; 7 (1):srep43185.

Chicago/Turabian Style

Shinsuke Seki; Kazunari Kusano; Seungki Lee; Yoshiko Iwasaki; Masaru Yagisawa; Mariko Ishida; Tadashi Hiratsuka; Takao Sasado; Kiyoshi Naruse; Goro Yoshizaki. 2017. "Production of the medaka derived from vitrified whole testes by germ cell transplantation." Scientific Reports 7, no. 1: srep43185.

Journal article
Published: 01 October 2016 in Cryobiology
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Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years possessed the ability to differentiate into functional eggs or sperm in the gonads of triploid recipient masu salmon (O. masou). After cryopreservation for 5 years in liquid nitrogen, intraperitoneally transplanted spermatogonia migrated toward, and incorporated into, the gonads of xenogeneic recipients. The transplanted spermatogonia resumed spermatogenesis and oogenesis in male and female recipients, respectively, and differentiated into sperm or eggs within the gonads of male and female recipients at 2 years posttransplantation. The differentiated sperm and eggs generated normal rainbow trout representative of donor phenotypes. Thus, cryopreservation of spermatogonia is a powerful and reliable method for long-term preservation of fish genetic resources.

ACS Style

Seungki Lee; Yoshiko Iwasaki; Goro Yoshizaki. Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids. Cryobiology 2016, 73, 286 -290.

AMA Style

Seungki Lee, Yoshiko Iwasaki, Goro Yoshizaki. Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids. Cryobiology. 2016; 73 (2):286-290.

Chicago/Turabian Style

Seungki Lee; Yoshiko Iwasaki; Goro Yoshizaki. 2016. "Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids." Cryobiology 73, no. 2: 286-290.

Journal article
Published: 01 September 2016 in Biochemical and Biophysical Research Communications
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Cryopreservation of fish sperm offers the practical applications in the selective breeding and biodiversity conservation. However, because of the lack of cryopreservation methods for fish eggs and embryos, maternally inherited cytoplasmic compartments cannot be successfully preserved. We previously developed an alternative method to derive functional eggs and sperm from cryopreserved whole testis by transplanting testicular cells into female and male recipients. However, if target fish had ovaries, the previous method employing male-derived germ cells would be ineffective. Here, we aimed to generate functional gametes from cryopreserved whole ovaries by transplanting ovarian germ cells into peritoneal cavity of sterile hatchlings. Cryopreservation conditions for rainbow trout ovaries (1.0 M DMSO, 0.1 M trehalose, and 10% egg yolk) were optimized by testing several different cryoprotective agents. Ovarian germ cells from thawed ovaries were intraperitoneally transplanted into allogeneic triploid hatchlings. Transplanted germ cells migrated toward and were incorporated into recipient gonads, where they underwent gametogenesis. Transplantation efficiency of ovarian germ cells remained stable after cryopreservation period up to 1185 days. Although all triploid recipients that did not undergo transplantation were functionally sterile, 5 of 25 female recipients and 7 of 25 male recipients reached sexual maturity at 2.5 years post-transplantation. Inseminating the resultant eggs and sperm generated viable offspring displaying the donor characteristics of orange body color, green fluorescence, and chromosome numbers. This method is thus a breakthrough tool for the conservation of endangered fish species that are crucial to cryopreserve the genetic resources of female fish.

ACS Style

Seungki Lee; Naoto Katayama; Goro Yoshizaki. Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells. Biochemical and Biophysical Research Communications 2016, 478, 1478 -1483.

AMA Style

Seungki Lee, Naoto Katayama, Goro Yoshizaki. Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells. Biochemical and Biophysical Research Communications. 2016; 478 (3):1478-1483.

Chicago/Turabian Style

Seungki Lee; Naoto Katayama; Goro Yoshizaki. 2016. "Generation of juvenile rainbow trout derived from cryopreserved whole ovaries by intraperitoneal transplantation of ovarian germ cells." Biochemical and Biophysical Research Communications 478, no. 3: 1478-1483.

Journal article
Published: 01 April 2016 in Cryobiology
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Because of the lack of cryopreservation techniques for fish eggs and embryos, cryopreservation of fish spermatogonia and subsequent generation of eggs and sperm would be an exit strategy for the long-term preservation of genetic resources. This study aimed to optimize cryoprotectants, cooling rates, and thawing temperatures for slow freezing of spermatogonia from endangered Manchurian trout (Brachymystax lenok). Whole testes were frozen with a cryomedium containing 1.3 M methanol, 0.2 M trehalose, and 10% egg yolk at a cooling rate of −1 °C/min and then stored in liquid nitrogen for 2 days. After thawing at 30 °C in a water bath, testicular cells from thawed testes were intraperitoneally transplanted into allogeneic triploid hatchlings. Transplanted spermatogonia migrated toward and were incorporated into recipient gonads, where they underwent gametogenesis. Transplantation efficiency did not significantly differ between frozen and fresh testes, demonstrating that Manchurian trout spermatogonia can be successfully cryopreserved in liquid nitrogen.

ACS Style

Seungki Lee; Goro Yoshizaki. Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok). Cryobiology 2016, 72, 165 -168.

AMA Style

Seungki Lee, Goro Yoshizaki. Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok). Cryobiology. 2016; 72 (2):165-168.

Chicago/Turabian Style

Seungki Lee; Goro Yoshizaki. 2016. "Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok)." Cryobiology 72, no. 2: 165-168.

Mitogenome announcement
Published: 01 January 2016 in Mitochondrial DNA Part B
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The Korean flying fish, Cheilopogon doederleinii, is a marine fish species belonging to the family Exocoetidae. In this study, I report for the first time the sequencing and assembly of the complete mitochondrial genome of C. doederleinii. The complete mitochondrial genome is 16,525 bp long and includes 13 protein-coding, 22 tRNA, and 2 rRNA genes. It has the typical vertebrate mitochondrial gene arrangement. Phylogenetic analysis using mitochondrial genomes of 10 species showed that C. doederleinii is clustered with C. arcticeps and grouped with the other Exocoetidae species. This mitochondrial genome provides potentially important resources for addressing taxonomic issues and studying molecular evolution.

ACS Style

Seungki Lee. Complete mitochondrial genome of the Korean flying fish Cheilopogon doederleinii (Beloniformes, Exocoetidae): mitogenome characterization and phylogenetic analysis. Mitochondrial DNA Part B 2016, 1, 901 -902.

AMA Style

Seungki Lee. Complete mitochondrial genome of the Korean flying fish Cheilopogon doederleinii (Beloniformes, Exocoetidae): mitogenome characterization and phylogenetic analysis. Mitochondrial DNA Part B. 2016; 1 (1):901-902.

Chicago/Turabian Style

Seungki Lee. 2016. "Complete mitochondrial genome of the Korean flying fish Cheilopogon doederleinii (Beloniformes, Exocoetidae): mitogenome characterization and phylogenetic analysis." Mitochondrial DNA Part B 1, no. 1: 901-902.

Mitogenome announcement
Published: 01 January 2016 in Mitochondrial DNA Part B
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The Korean oily shinner (Sarcocheilichthys nigripinnis morii) is a subspecies belonging to the subfamily Gobioninae in the family Cyprinidae and is endemic to Korea. Herein, we report the first sequencing and assembly of the complete mitochondrial genome of S. nigripinnis morii. The complete mitochondrial genome is 16,679 bp long, consisting of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. It has the typical vertebrate mitochondrial gene arrangement. Phylogenetic analysis using mitogenomes of 16 species showed that S. nigripinnis morii was clustered with S. nigripinnis and S. variegatus microoculus and grouped with the other Cyprinidae species.

ACS Style

Seungki Lee; Ha Yeun Song. Complete mitochondrial genome of the Korean oily shinner Sarcocheilichthys nigripinnis morii (Cypriniformes, cyprinidae): mitogenome characterization and phylogenetic analysis. Mitochondrial DNA Part B 2016, 1, 837 -838.

AMA Style

Seungki Lee, Ha Yeun Song. Complete mitochondrial genome of the Korean oily shinner Sarcocheilichthys nigripinnis morii (Cypriniformes, cyprinidae): mitogenome characterization and phylogenetic analysis. Mitochondrial DNA Part B. 2016; 1 (1):837-838.

Chicago/Turabian Style

Seungki Lee; Ha Yeun Song. 2016. "Complete mitochondrial genome of the Korean oily shinner Sarcocheilichthys nigripinnis morii (Cypriniformes, cyprinidae): mitogenome characterization and phylogenetic analysis." Mitochondrial DNA Part B 1, no. 1: 837-838.

Mitogenome announcement
Published: 01 January 2016 in Mitochondrial DNA Part B
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The mud loach (Misgurnus mizolepis) is a small benthic species belonging to the family Cobitidae. In this study, I report the first sequencing and assembly of the complete mitochondrial genome of M. mizolepis. The complete mitochondrial genome is 16,647 bp long, consisting of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and a control region. It has the typical vertebrate mitochondrial gene arrangement. Phylogenetic analysis using mitochondrial genomes of 20 species showed that M. mizolepis is clustered with M. anguillicaudatus and M. bipartitus. This mitochondrial genome provides potentially important resources for studying molecular evolution and biogeography.

ACS Style

Seungki Lee. Complete mitochondrial genome of the mud loach Misgurnus mizolepis (Cypriniformes, Cobitidae) and its phylogenetic position in the Cypriniformes. Mitochondrial DNA Part B 2016, 1, 839 -840.

AMA Style

Seungki Lee. Complete mitochondrial genome of the mud loach Misgurnus mizolepis (Cypriniformes, Cobitidae) and its phylogenetic position in the Cypriniformes. Mitochondrial DNA Part B. 2016; 1 (1):839-840.

Chicago/Turabian Style

Seungki Lee. 2016. "Complete mitochondrial genome of the mud loach Misgurnus mizolepis (Cypriniformes, Cobitidae) and its phylogenetic position in the Cypriniformes." Mitochondrial DNA Part B 1, no. 1: 839-840.