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I have undergraduate Bachelor's Degree in Biological Sciences from the State University of Paraíba (2004) and Master's degree in Agronomy from the Federal University of Paraíba (2006). In 2010, I completed a Doctorate in Plant Biotechnology at Federal University of Rio de Janeiro, with the theme Microorganisms Growth Promoters in plants. I Postdoctoral in the field of Proteomics by the National Institute of Science and Technology in Biological Nitrogen Fixation-CNPq from 2010 to 2011. From 2011 to 2012 I worked again as a Fellow Postdoctoral FAPERJ-EMBRAPA Agrobiology in the area of microorganisms Genomics transcriptomics. Approved by public tender at the end of 2011, I joined the professor career in Paraiba State University in April 2012, currently standing exercising my activities in the Department of Biology, the Center for Biological and Health Sciences, Campus I located in the city of Campina Grande – PB.
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopathogenic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 α-helices and 11 β-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 μg μl−1). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. albilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.
Elizabeth Teixeira De Almeida Ramos; Carlos Henrique Salvino Gadelha Meneses; Marcia Soares Vidal; José Ivo Baldani. Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. Annals of Applied Biology 2021, 1 .
AMA StyleElizabeth Teixeira De Almeida Ramos, Carlos Henrique Salvino Gadelha Meneses, Marcia Soares Vidal, José Ivo Baldani. Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. Annals of Applied Biology. 2021; ():1.
Chicago/Turabian StyleElizabeth Teixeira De Almeida Ramos; Carlos Henrique Salvino Gadelha Meneses; Marcia Soares Vidal; José Ivo Baldani. 2021. "Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans." Annals of Applied Biology , no. : 1.
Salinity in soil can affect Jatropha seedling metabolism, interfering with plant establishment. In this study, the effect of salinity on the mobilization of reserves during the development of Jatropha seedlings was tested. Two genotypes of Jatropha were used and three concentrations of NaCl were applied between the 4th and 8th days after germination. The effects of salinity on seedling growth, in terms of fresh and dry phytomass, ionic partition, and sugar quantification, starch, proteins, amino acids, and lipids were evaluated in cotyledon leaves, hypocotyls, and roots. There was an increase in the content of all classes of macromolecules analyzed in at least one of the organs. It is hypothesized that the hypocotyls acted as an accumulating organ of Na+. The accumulations of amino acids and protein in roots suggest that metabolic responses occurred in response to the ionic and osmotic effects of NaCl, although this accumulation did not appear to prevent biomass losses in seedlings. Furthermore, the findings of this study demonstrate that salinity inhibits the mobilization of lipids and carbon stocks from cotyledon leaves to the rest of the plant, and together with the synthesis of proteins and amino acids that occurred primarily in roots, contributed to response of these plants to salinity.
Emannuella Lira; Joilma Souza; Lucas Galdino; Cristiane Macêdo; Anselmo Silva; Yuri Melo; Ivanice Santos; Nair Arriel; Carlos Meneses; Josemir Maia. Changes in Reserve Mobilization Caused by Salinity Could Interfere in the Initial Growth of Jatropha curcas. Sustainability 2021, 13, 7446 .
AMA StyleEmannuella Lira, Joilma Souza, Lucas Galdino, Cristiane Macêdo, Anselmo Silva, Yuri Melo, Ivanice Santos, Nair Arriel, Carlos Meneses, Josemir Maia. Changes in Reserve Mobilization Caused by Salinity Could Interfere in the Initial Growth of Jatropha curcas. Sustainability. 2021; 13 (13):7446.
Chicago/Turabian StyleEmannuella Lira; Joilma Souza; Lucas Galdino; Cristiane Macêdo; Anselmo Silva; Yuri Melo; Ivanice Santos; Nair Arriel; Carlos Meneses; Josemir Maia. 2021. "Changes in Reserve Mobilization Caused by Salinity Could Interfere in the Initial Growth of Jatropha curcas." Sustainability 13, no. 13: 7446.
Drought is a global problem for crop productivity. Therefore, the objective of this research was to evaluate five dry-Caribbean Bacillus spp. strains in drought stress amelioration in maize plants. Maize seeds were single-strain inoculated and sown in pots under greenhouse conditions. After 12 days, plants were subjected to 33 days of drought conditions, i.e., 30% of soil field capacity, and then collected to measure leaf and root dry biomass, plant height, antioxidant enzymes, proline accumulation, and P+, Ca2+, and K+ uptake. Results correlated drought stress amelioration with the inoculation of Bacillus spp. strains XT13, XT38 and XT110. Inoculated plants showed increases in dry biomass, plant height, and K+ and P+ uptake. The overall maize antioxidant response to bacterial inoculation under drought stress showed dependence on proline accumulation and decreases in ascorbate peroxidase and glutathione reductase activities. Moreover, results suggest that this stress amelioration is driven by a specific plant-strain correlation observed in antioxidant response changes in inoculated plants under stress. Also, there is a complex integration of several mechanisms, including plant growth-promotion traits and nutrient uptake. Hence, the use of dry-Caribbean plant growth-promoting Bacillus strains represents an important biotechnological approach to enhance crop productivity in arid and semi-arid environments.
Andres Moreno-Galván; Felipe A. Romero-Perdomo; German Estrada-Bonilla; Carlos Henrique Salvino Gadelha Meneses; Ruth R. Bonilla. Dry-Caribbean Bacillus spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation. Microorganisms 2020, 8, 1 .
AMA StyleAndres Moreno-Galván, Felipe A. Romero-Perdomo, German Estrada-Bonilla, Carlos Henrique Salvino Gadelha Meneses, Ruth R. Bonilla. Dry-Caribbean Bacillus spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation. Microorganisms. 2020; 8 (6):1.
Chicago/Turabian StyleAndres Moreno-Galván; Felipe A. Romero-Perdomo; German Estrada-Bonilla; Carlos Henrique Salvino Gadelha Meneses; Ruth R. Bonilla. 2020. "Dry-Caribbean Bacillus spp. Strains Ameliorate Drought Stress in Maize by a Strain-Specific Antioxidant Response Modulation." Microorganisms 8, no. 6: 1.
Rhizobia are common members of plant microbiomes. This study aimed to evaluate if rhizobia can colonize sugarcane mini-setts and if and how they affect sprouting and morphological traits of plant development. Bradyrhizobium sacchari strain BR 10280T and Rhizobium sp. strain BR 10268 were inoculated on sugarcane mini-setts and sprouting and plant development were evaluated. Bacterial production of hormones and their effect on plant growth were studied using HPLC and GC-MS / SIM and sugarcane sprouting assays. Colonization was investigated by colony counting and CLSM. Sequence analysis of recA, gyrB and rpoB genes was applied to refine the phylogenetic classification of strain BR 10268. BR 10268 had a positive effect on sugarcane mini-sett sprouting and shoot and root growth at 30 and 75 days after transplantation (DAT) and colonized sprouting mini-sett tissues. Few significant effects were observed for B. sacchari BR 10280T. Cell-free BR 10268 supernatant was found to contain gibberellins (GAs) and low concentrations of indole-3-acetic acid (IAA) and trans-zeatin; treatment of mini-setts with GA3 (0.15 or 1.5 μg mL−1) affected sprouting in a similar manner as BR 10268 inoculation. BR 10268 was found to be phylogenetically close to R. freirei. Rhizobium sp. BR 10268 positively affects sprouting and growth of sugarcane, probably via production of plant hormonal substances, notably gibberellins, thus naturally occurring rhizobia may have potential as sugarcane inoculants.
Natália S. Ferreira; Gustavo F. Matos; Carlos Meneses; Veronica M. Reis; Janaina R. C. Rouws; Stefan Schwab; José I. Baldani; Luc F. M. Rouws. Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development. Plant and Soil 2020, 451, 221 -238.
AMA StyleNatália S. Ferreira, Gustavo F. Matos, Carlos Meneses, Veronica M. Reis, Janaina R. C. Rouws, Stefan Schwab, José I. Baldani, Luc F. M. Rouws. Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development. Plant and Soil. 2020; 451 (1-2):221-238.
Chicago/Turabian StyleNatália S. Ferreira; Gustavo F. Matos; Carlos Meneses; Veronica M. Reis; Janaina R. C. Rouws; Stefan Schwab; José I. Baldani; Luc F. M. Rouws. 2020. "Interaction of phytohormone-producing rhizobia with sugarcane mini-setts and their effect on plant development." Plant and Soil 451, no. 1-2: 221-238.
Background: Inoculation with Gluconacetobacter diazotrophicus has shown to influence root development in red rice plants, and more recently, the induced systemic tolerance (IST) response to drought was also demonstrated. The goal of this study was to evaluate the inoculation effect of G. diazotrophicus strain Pal5 on the amelioration of drought stress and root development in red rice (Oryza sativa L.). Methods: The experimental treatments consist of red rice plants inoculated with and without strain Pal5 in presence and absence of water restriction. Physiological, biochemical, and molecular analyses of plant roots were carried out, along with measurements of growth and biochemical components. Results: The plants showed a positive response to the bacterial inoculation, with root growth promotion and induction of tolerance to drought. An increase in the root area and higher levels of osmoprotectant solutes were observed in roots. Bacterial inoculation increased the drought tolerance and positively regulated certain root development genes against the water deficit in plants. Conclusion: G. diazotrophicus Pal5 strain inoculation favored red rice plants by promoting various root growth and developmental mechanisms against drought stress, enabling root development and improving biochemical composition.
Renata Silva; Luanna Filgueiras; Bruna Santos; Mariana Coelho; Maria Silva; Germán Estrada-Bonilla; Marcia Vidal; José Ivo Baldani; Carlos Meneses. Gluconacetobacter diazotrophicus Changes The Molecular Mechanisms of Root Development in Oryza sativa L. Growing Under Water Stress. International Journal of Molecular Sciences 2020, 21, 333 .
AMA StyleRenata Silva, Luanna Filgueiras, Bruna Santos, Mariana Coelho, Maria Silva, Germán Estrada-Bonilla, Marcia Vidal, José Ivo Baldani, Carlos Meneses. Gluconacetobacter diazotrophicus Changes The Molecular Mechanisms of Root Development in Oryza sativa L. Growing Under Water Stress. International Journal of Molecular Sciences. 2020; 21 (1):333.
Chicago/Turabian StyleRenata Silva; Luanna Filgueiras; Bruna Santos; Mariana Coelho; Maria Silva; Germán Estrada-Bonilla; Marcia Vidal; José Ivo Baldani; Carlos Meneses. 2020. "Gluconacetobacter diazotrophicus Changes The Molecular Mechanisms of Root Development in Oryza sativa L. Growing Under Water Stress." International Journal of Molecular Sciences 21, no. 1: 333.
The endophytic diazotrophic strain CBAmC of Nitrospirillum amazonense has been reported as a plant growth promoter of sugarcane variety RB867515 when grown under field conditions. The present work aimed to assess the influence of apoplast fluid from RB867515 on the transcriptomic and proteomic profiles of CBAmC cultured in vitro. RNA-Seq in Ion Proton™ and ESI-LC-MS/MS peptide analysis were used to evaluate the transcriptomic and proteomic profiles, respectively, of CBAmC exposed for 2 h to the sugarcane apoplast fluid. The bacterial transcriptomic and proteomic profiles were well correlated. The overall response of CBAmC to the apoplast fluid included overexpression of defense systems against reactive oxygen species (ROS) and osmotic stress, RND efflux pumps for toxic compounds, Sec and Tat secretory systems, and assimilative metabolism of iron. In contrast, active transporters of organic compounds, chemotaxis system and flagellum structure were underexpressed. The bacterial metabolic pathways / functions activated in response to the sugarcane apoplast fluid are most likely related to its adaptation to the peculiar characteristics of the fluid. The activation of some of those functions could be determinant for its adaptation to the sugarcane apoplastic niche, and perhaps be involved in the previously observed effect of promoting plant growth.
Leonardo A. Terra; Cleiton P. de Soares; Carlos H. S. G. Meneses; Michelle Z. Tadra Sfeir; Emanuel M. de Souza; Vanildo Silveira; Márcia S. Vidal; José I. Baldani; Stefan Schwab. Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid. Plant and Soil 2019, 451, 145 -168.
AMA StyleLeonardo A. Terra, Cleiton P. de Soares, Carlos H. S. G. Meneses, Michelle Z. Tadra Sfeir, Emanuel M. de Souza, Vanildo Silveira, Márcia S. Vidal, José I. Baldani, Stefan Schwab. Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid. Plant and Soil. 2019; 451 (1-2):145-168.
Chicago/Turabian StyleLeonardo A. Terra; Cleiton P. de Soares; Carlos H. S. G. Meneses; Michelle Z. Tadra Sfeir; Emanuel M. de Souza; Vanildo Silveira; Márcia S. Vidal; José I. Baldani; Stefan Schwab. 2019. "Transcriptome and proteome profiles of the diazotroph Nitrospirillum amazonense strain CBAmC in response to the sugarcane apoplast fluid." Plant and Soil 451, no. 1-2: 145-168.
Plant growth promoting bacteria (PGPB) are associated with plant roots and augment plant productivity and immunity; however, recent work by several groups has shown that PGPB also elicit the so-called “induced systemic tolerance” to drought stress. This work aimed to evaluate the effect of Gluconacetobacter diazotrophicus Pal5 inoculation on the mitigation of drought stress in red rice (Oryza sativa L.). The experiment treatments of the red rice cultivar inoculated and uninoculated with G. diazotrophicus, and cultivated with and without water restriction. Physiological, biochemical, and molecular analyses of plant tissues were carried out, along with measurements of biomass and seed yield components. The plants showed a positive response to inoculation with G. diazotrophicus, with promotion of growth and induction of tolerance to drought. Increase in plant biomass and higher levels of gas exchange and osmoprotectant solutes were observed in shoots. The bacterial inoculation increased the yield and positively regulated some defense genes against the water deficit in plants. G. diazotrophicus Pal5 strain inoculation was beneficial for red rice plants, suppressing the various deleterious effects of drought stress and increasing the biomass and seed yield components.
Luanna Filgueiras; Renata Silva; Isabela Almeida; Marcia Vidal; José Ivo Baldani; Carlos Henrique Salvino Gadelha Meneses. Gluconacetobacter diazotrophicus mitigates drought stress in Oryza sativa L. Plant and Soil 2019, 451, 57 -73.
AMA StyleLuanna Filgueiras, Renata Silva, Isabela Almeida, Marcia Vidal, José Ivo Baldani, Carlos Henrique Salvino Gadelha Meneses. Gluconacetobacter diazotrophicus mitigates drought stress in Oryza sativa L. Plant and Soil. 2019; 451 (1-2):57-73.
Chicago/Turabian StyleLuanna Filgueiras; Renata Silva; Isabela Almeida; Marcia Vidal; José Ivo Baldani; Carlos Henrique Salvino Gadelha Meneses. 2019. "Gluconacetobacter diazotrophicus mitigates drought stress in Oryza sativa L." Plant and Soil 451, no. 1-2: 57-73.
It has been suggested that fatty acid balance in standard sunflower oil may be influenced by the genotype of the plant or by variation in environmental growth conditions. Therefore, this study analysed aspects of sunflower productivity and oil quality obtained from achenes of plants cultivated in a semi-arid environment, resulting from seed obtained from other regions with different edaphoclimatic characteristics. The experiment was conducted between February and May, in a Brazilian semi-arid area. At 90 days after sowing (DAS), the physiological maturation period, tissue was harvested, and the yield of plant green matter, oil and oil per hectare was evaluated. A comparative analysis of the oil content of achenes before and after planting (parental v progeny) was performed. The experiment was conducted in a randomized block design of 4 treatments (genotypes), Embrapa122, BRSG01, Helio253 and Helio250, and 4 replications, for a total of 16 experimental plots, with each plot constituting 8 plants. In this study, sunflowers were produced in the climatic conditions of the Brazilian semi-arid region with biomasses, achenes and oil contents that were different from other crop productions in the country, providing the possibility of obtaining oil with a distinct chemical composition.
Valéria Fernandes De Oliveira Sousa; Gisele Lopes Dos Santos; Josemir Moura Maia; Carlos Meneses; Marília Hortência Batista Silva Rodrigues; Thiago Jardelino Dias. Edaphoclimatic Conditions of the Brazilian Semi-Arid Region Affect the Productivity and Composition of Sunflower Oil. Journal of Agricultural Studies 2019, 7, 309 -322.
AMA StyleValéria Fernandes De Oliveira Sousa, Gisele Lopes Dos Santos, Josemir Moura Maia, Carlos Meneses, Marília Hortência Batista Silva Rodrigues, Thiago Jardelino Dias. Edaphoclimatic Conditions of the Brazilian Semi-Arid Region Affect the Productivity and Composition of Sunflower Oil. Journal of Agricultural Studies. 2019; 7 (2):309-322.
Chicago/Turabian StyleValéria Fernandes De Oliveira Sousa; Gisele Lopes Dos Santos; Josemir Moura Maia; Carlos Meneses; Marília Hortência Batista Silva Rodrigues; Thiago Jardelino Dias. 2019. "Edaphoclimatic Conditions of the Brazilian Semi-Arid Region Affect the Productivity and Composition of Sunflower Oil." Journal of Agricultural Studies 7, no. 2: 309-322.
Red rice is cultivated mainly by small farmers in the northeastern region of Brazil, a region characterized by water scarcity, that is why studies like those are needed aiming to save water without undermining crop cultivation. In this context, the objective was to evaluate in the red rice crop with application of silicon and biofertilizer: soil water levels, growth, productivity and biochemical parameters. The experiment was conducted in a protected environment at the State University of Paraíba, in the municipality of Catolé do Rocha-PB. The design was completely randomized, in the factorial arrangement 4 x 2 x 2, with four replications. The treatments consisted of four levels of water in the soil (70, 80, 90 and 100%), with silicon and without silicon application, with biofertilizer and without biofertilizer application. Rice responded significantly to available water levels in the soil for all variables studied. Silicon and biofertilizer contributed to a better tolerance of rice to water stress. Organomineral fertilization increased the concentrations of the Proline, Glycine betaine and Trehalose osmoregulators. The lowest availability of water provided an increasement in the red rice osmoregulators concentration, that act as a plants protector against cell damages.
Katia Otilia Gomes Dutra; Toni Halan Da Silva Irineu; Mário Leno Martins Véras; Janailson Pereira De Figueredo; Josimar Nogueora Da Silva; Raimundo Andrade; Carlos Henrique Salvino Gadelha Meneses. Biochemical alterations of red rice cultivated at soil water levels and organomineral fertilization. Comunicata Scientiae 2018, 9, 185 -193.
AMA StyleKatia Otilia Gomes Dutra, Toni Halan Da Silva Irineu, Mário Leno Martins Véras, Janailson Pereira De Figueredo, Josimar Nogueora Da Silva, Raimundo Andrade, Carlos Henrique Salvino Gadelha Meneses. Biochemical alterations of red rice cultivated at soil water levels and organomineral fertilization. Comunicata Scientiae. 2018; 9 (2):185-193.
Chicago/Turabian StyleKatia Otilia Gomes Dutra; Toni Halan Da Silva Irineu; Mário Leno Martins Véras; Janailson Pereira De Figueredo; Josimar Nogueora Da Silva; Raimundo Andrade; Carlos Henrique Salvino Gadelha Meneses. 2018. "Biochemical alterations of red rice cultivated at soil water levels and organomineral fertilization." Comunicata Scientiae 9, no. 2: 185-193.
Alkaline phytases from uncultured microorganisms, which hydrolyze phytate to less phosphorylated myo-inositols and inorganic phosphate, have great potential as additives in agricultural industry. The development of metagenomics has stemmed from the ineluctable evidence that as-yet-uncultured microorganisms represent the vast majority of organisms in most environments on earth. In this study, a gene encoding a phytase was cloned from red rice crop residues and castor bean cake using a metagenomics strategy. The amino acid identity between this gene and its closest published counterparts is lower than 60%. The phytase was named PhyRC001 and was biochemically characterized. This recombinant protein showed activity on sodium phytate, indicating that PhyRC001 is a hydrolase enzyme. The enzymatic activity was optimal at a pH of 7.0 and at a temperature of 35 °C. β-propeller phytases possess great potential as feed additives because they are the only type of phytase with high activity at neutral pH. Therefore, to explore and exploit the underlying mechanism for β-propeller phytase functions could be of great benefit to biotechnology.
Nathálya Farias; Isabela Almeida; Carlos Meneses. New Bacterial Phytase through Metagenomic Prospection. Molecules 2018, 23, 448 .
AMA StyleNathálya Farias, Isabela Almeida, Carlos Meneses. New Bacterial Phytase through Metagenomic Prospection. Molecules. 2018; 23 (2):448.
Chicago/Turabian StyleNathálya Farias; Isabela Almeida; Carlos Meneses. 2018. "New Bacterial Phytase through Metagenomic Prospection." Molecules 23, no. 2: 448.
The endophytic bacterium Gluconacetobacter diazotrophicus produces exopolysaccharides (EPS) that are required for biofilm formation and colonization of rice seedlings. Here we investigated whether EPS produced by the G. diazotrophicus strain Pal5 protects the bacteria against free radicals. EPS-mediated protection of Pal5 cells against oxidative damage was evaluated by cell counting and fluorescence microscopy. Rice seedling inoculation studies were performed to investigate the antioxidant activity of EPS during plant colonization. The expression of three bacterial antioxidant genes during plant colonization was also monitored. Free radical activities were reduced in vitro by the addition of Pal5 EPS. An EPS-defective Pal5 mutant was hypersensitive to H2O2 and addition of purified EPS reversed this phenotype. Addition of EPS at the inoculation time increased colonization efficiency by the mutant strain and a similar effect was observed after addition of the antioxidant ascorbic acid. qPCR profiles of sodA, gor, and katE gene expression in the mutant confirmed the role of EPS during the initial plant colonization. Our results indicated that EPS produced by G. diazotrophicus protects the bacterial cells against oxidative stress in vitro and during colonization of rice plants.
C. Meneses; T. Gonçalves; S. Alquéres; L. Rouws; Rodrigo Serrato; M. Vidal; J. I. Baldani. Gluconacetobacter diazotrophicus exopolysaccharide protects bacterial cells against oxidative stress in vitro and during rice plant colonization. Plant and Soil 2017, 416, 133 -147.
AMA StyleC. Meneses, T. Gonçalves, S. Alquéres, L. Rouws, Rodrigo Serrato, M. Vidal, J. I. Baldani. Gluconacetobacter diazotrophicus exopolysaccharide protects bacterial cells against oxidative stress in vitro and during rice plant colonization. Plant and Soil. 2017; 416 (1-2):133-147.
Chicago/Turabian StyleC. Meneses; T. Gonçalves; S. Alquéres; L. Rouws; Rodrigo Serrato; M. Vidal; J. I. Baldani. 2017. "Gluconacetobacter diazotrophicus exopolysaccharide protects bacterial cells against oxidative stress in vitro and during rice plant colonization." Plant and Soil 416, no. 1-2: 133-147.
To evaluate the photosynthetic efficiency, gas exchange and yield of castor bean intercropped with peanut under Brazilian semiarid conditions, a field experiment was set up with six treatments and four replicates arranged in a randomized block design. The experiment consisted of the cultivation of castor bean ‘BRS Energia’ in a monocrop system and intercropped with peanut ‘BR 1’. One treatment was formed by monocropped castor bean and the others by the castor bean intercropped with peanut with different intercrop establishment times, i.e. peanut sowing times (0, 10, 15, 20 and 25 days after castor bean sowing). The castor bean photosynthetic and carboxylation efficiencies were higher in the intercrop system with a 25-day interval between sowings. Peanut sowing at 0 to 10 days after castor bean sowing in the intercropped system affected the photochemical efficiency of the latter, which resulted in its yield reduction. Larger intervals between sowing of peanut ‘BR-1’ and of the main crop increased castor bean yield in the intercrop system. Key words: chlorophyll fluorescence; photosynthesis; Ricinus communis L.; yield
Alexson Filgueiras Dutra; Alberto S. De Melo; Marcos E. B. Brito; Luanna M. B. Filgueiras; Carlos H. S. G. Meneses. Photosynthetic efficiency, gas exchange and yield of castor bean intercropped with peanut in semiarid Brazil. Revista Brasileira de Engenharia Agrícola e Ambiental 2017, 21, 106 -110.
AMA StyleAlexson Filgueiras Dutra, Alberto S. De Melo, Marcos E. B. Brito, Luanna M. B. Filgueiras, Carlos H. S. G. Meneses. Photosynthetic efficiency, gas exchange and yield of castor bean intercropped with peanut in semiarid Brazil. Revista Brasileira de Engenharia Agrícola e Ambiental. 2017; 21 (2):106-110.
Chicago/Turabian StyleAlexson Filgueiras Dutra; Alberto S. De Melo; Marcos E. B. Brito; Luanna M. B. Filgueiras; Carlos H. S. G. Meneses. 2017. "Photosynthetic efficiency, gas exchange and yield of castor bean intercropped with peanut in semiarid Brazil." Revista Brasileira de Engenharia Agrícola e Ambiental 21, no. 2: 106-110.
Many naturally-occurring cellulolytic microorganisms are not readily cultivable, demanding a culture-independent approach in order to study their cellulolytic genes. Metagenomics involves the isolation of DNA from environmental sources and can be used to identify enzymes with biotechnological potential from uncultured microbes. In this study, a gene encoding an endoglucanase was cloned from red rice crop residues using a metagenomic strategy. The amino acid identity between this gene and its closest published counterparts is lower than 70%. The endoglucanase was named EglaRR01 and was biochemically characterized. This recombinant protein showed activity on carboxymethylcellulose, indicating that EglaRR01 is an endoactive lytic enzyme. The enzymatic activity was optimal at a pH of 6.8 and at a temperature of 30 °C. Ethanol production from this recombinant enzyme was also analyzed on EglaRR01 crop residues, and resulted in conversion of cellulose from red rice into simple sugars which were further fermented by Saccharomyces cerevisiae to produce ethanol after seven days. Ethanol yield in this study was approximately 8 g/L. The gene found herein shows strong potential for use in ethanol production from cellulosic biomass (second generation ethanol).
Carlos Meneses; Bruna Silva; Betsy Dantas de Medeiros; Rodrigo Serrato; David Johnston-Monje. A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues. Molecules 2016, 21, 831 .
AMA StyleCarlos Meneses, Bruna Silva, Betsy Dantas de Medeiros, Rodrigo Serrato, David Johnston-Monje. A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues. Molecules. 2016; 21 (7):831.
Chicago/Turabian StyleCarlos Meneses; Bruna Silva; Betsy Dantas de Medeiros; Rodrigo Serrato; David Johnston-Monje. 2016. "A Metagenomic Advance for the Cloning and Characterization of a Cellulase from Red Rice Crop Residues." Molecules 21, no. 7: 831.
In regions where rainfall is limited and irregular, irrigation technique is important for optimizing fruit plants production and profitability, such as yellow passion fruit. Therefore, this study investigates the economic viability of irrigations depth applied on passion fruit cultivation in the semiarid region of Paraíba State. The treatments were arranged on a factorial scheme that comprises four levels of ETo replacement (33, 66, 100 and 133%) and two yellow passion fruit hybrids (BRS Sol do Cerrado and BRS Gigante Amarelo), in a randomized block design, with five replications. Effective operational cost, administrative cost, total water cost, total production cost, gross income, net income, benefit/cost relation, balance of price and profitability index were evaluated. The best economic results were achieved with ‘BRS Sol do Cerrado’ hybrid under irrigation with 133% of ETo. Key words: Passiflora edulis, hybrids, economic viability, drip irrigation.
Madson Da Silva Jos Eacute; Soares De Melo Alberto; Filgueiras Dutra Alexson; Fernandes Suassuna Janivan; Filgueiras Dutra Wellison; Henrique Salvino Gadelha Meneses Carlos; Roberto Almeida Vi Eacute Gas Pedro; José Madson Da Silva; Alberto Soares De Melo; Alexson Filgueiras Dutra; Janivan Fernandes Suassuna; Carlos Meneses; Pedro Roberto Almeida Viégas. Profitability of yellow passion fruit as a function of irrigation depths under semiarid conditions. African Journal of Agricultural Research 2016, 11, 1079 -1085.
AMA StyleMadson Da Silva Jos Eacute, Soares De Melo Alberto, Filgueiras Dutra Alexson, Fernandes Suassuna Janivan, Filgueiras Dutra Wellison, Henrique Salvino Gadelha Meneses Carlos, Roberto Almeida Vi Eacute Gas Pedro, José Madson Da Silva, Alberto Soares De Melo, Alexson Filgueiras Dutra, Janivan Fernandes Suassuna, Carlos Meneses, Pedro Roberto Almeida Viégas. Profitability of yellow passion fruit as a function of irrigation depths under semiarid conditions. African Journal of Agricultural Research. 2016; 11 (12):1079-1085.
Chicago/Turabian StyleMadson Da Silva Jos Eacute; Soares De Melo Alberto; Filgueiras Dutra Alexson; Fernandes Suassuna Janivan; Filgueiras Dutra Wellison; Henrique Salvino Gadelha Meneses Carlos; Roberto Almeida Vi Eacute Gas Pedro; José Madson Da Silva; Alberto Soares De Melo; Alexson Filgueiras Dutra; Janivan Fernandes Suassuna; Carlos Meneses; Pedro Roberto Almeida Viégas. 2016. "Profitability of yellow passion fruit as a function of irrigation depths under semiarid conditions." African Journal of Agricultural Research 11, no. 12: 1079-1085.
The aim of this study was to evaluate the agronomics, physiological and biochemical characteristics of different peanut seeds and plant genotypes. The procedures have been performed during the secondary crop in the agricultural year 2013 to 2014, after the peanut harvest in Barbalha, Ceará – Brazil. The experiment was laid out in split-plot in randomized complete block design with four replicates, constituting scheme 4×5 factorial analysis, providing four levels of replacement of ET0 (40, 70, 100 and 130%) and five genotypes of peanut (BRS Perola Branca, L7 Beje, Runner, BRS Havana, BR 1), which were measured: number of leaves, total phytomass, and total chlorophyll content was performed, as well as hundred-seeds weight, pods weight per plant, number of pods per plant and seed yield per plant oil yield and fatty acid composition. It is shown herein that the interaction between peanut genotypes and water restriction levels promote a decrease in plant growth, physiological factors, and biochemical production of oil, possibly due to a decrease in the photosynthetic metabolism, whereas the plants exposed to water stress conditions modulated all ecophysiological responses in favor of a better performance in the harsh environment. The significant differences exhibited by different cultivars in this study for oil content, some productive characteristics and fatty acids compositions could be attributed to the genetic make-up of a particular cultivar (BR 1), its place of the environmental to reach high oil quality. Knowing better combination effects of climatic fluctuations and botanical types on fatty acid composition would be useful in designing management practices to obtain a specific oil quality and improving predictions of crop models in northeastern Brazil. Key words: Arachis hypogaea L., water deficiency, adaptability, tolerance.
Maria Silva Darlene; Luciano De Souza Ferraz Rener; Vassoler Serrato Rodrigo; Soares De Melo Alberto; Henrique Salvino Gadelha Meneses Carlos; Darlene Maria Silva; Rener Luciano De Souza Ferraz; Rodrigo Vassoler Serrato; Alberto Soares De Melo; Carlos Meneses. Growth, productivity and fatty acid composition of oils of peanut genotypes submitted to different levels of water replacement. African Journal of Agricultural Research 2015, 10, 3987 -3996.
AMA StyleMaria Silva Darlene, Luciano De Souza Ferraz Rener, Vassoler Serrato Rodrigo, Soares De Melo Alberto, Henrique Salvino Gadelha Meneses Carlos, Darlene Maria Silva, Rener Luciano De Souza Ferraz, Rodrigo Vassoler Serrato, Alberto Soares De Melo, Carlos Meneses. Growth, productivity and fatty acid composition of oils of peanut genotypes submitted to different levels of water replacement. African Journal of Agricultural Research. 2015; 10 (42):3987-3996.
Chicago/Turabian StyleMaria Silva Darlene; Luciano De Souza Ferraz Rener; Vassoler Serrato Rodrigo; Soares De Melo Alberto; Henrique Salvino Gadelha Meneses Carlos; Darlene Maria Silva; Rener Luciano De Souza Ferraz; Rodrigo Vassoler Serrato; Alberto Soares De Melo; Carlos Meneses. 2015. "Growth, productivity and fatty acid composition of oils of peanut genotypes submitted to different levels of water replacement." African Journal of Agricultural Research 10, no. 42: 3987-3996.
The Vigna bean is a crop of great socio-economic importance to Northeast Brazil, however, this region water scarcity is a common condition, directly interfering on bean yield. Thus, the objective of this study was to quantify the levels of proline, trehalose, total dry mass (TDM) and weight of pods (WP) of Vigna genotypes under different replacement rates of ETo. The experiment consisted of growing three genotypes Vigna (BRS Aracé, BR17 Gurguéia and BRS Marataoã) under application of four rates of water replacement (100, 80, 60 and 40% of ETo), totaling 12 treatments arranged in randomized blocks with four replications, and the useful portion comprising ten plants. The concentrations of proline and trehalose were higher in plants with water deficit, indicating that a mechanism of stress tolerance, possibly by acting as osmoprotectors. Plants with higher water deficit after rehydrated, decreased the concentration of proline and trehalose, but remained above the values obtained without water deficit. In genotype ‘BRS Marataoã’ the greater weight of pods were found. Trehalose and proline can be used as indicators of adaptability bean Vigna in water deficit conditions.
A.R.F. Silva; A.F. Dutra; V.G. Dias; C.H.S.G. Meneses; A.S. Melo. Proline Accumulation and Trehalose of Beans Cultivars Vigna under Water Deficit. II Inovagri International Meeting 2014, 5166 -5173.
AMA StyleA.R.F. Silva, A.F. Dutra, V.G. Dias, C.H.S.G. Meneses, A.S. Melo. Proline Accumulation and Trehalose of Beans Cultivars Vigna under Water Deficit. II Inovagri International Meeting. 2014; ():5166-5173.
Chicago/Turabian StyleA.R.F. Silva; A.F. Dutra; V.G. Dias; C.H.S.G. Meneses; A.S. Melo. 2014. "Proline Accumulation and Trehalose of Beans Cultivars Vigna under Water Deficit." II Inovagri International Meeting , no. : 5166-5173.
In the Brazilian Northeast, the importance of melon crop has stimulated the intensification of research on physiology, biochemistry and evaluation of gas exchange. In that sense, it was aimed to evaluate the effect of ascorbate peroxidase (APX), of catalase (CAT), superoxide dismutase (SOD) and proline genotype irrigated melon under different rates of evapotranspiration (ETo). The genotype was studied hybrid ‘Juazeiro’ of group Frog Skin grown under four different rates of water replacement (100, 80, 60 and 40% of ETo). The statistical experimental design was randomized blocks with five replications, and the useful portion consisting of five plants. The concentration of APX, CAT and SOD after water supplementation decreased, this is due to the fact that plants when submitted to water stress tend to increase the production of reactive oxygen species (ROS) now with proline, found a decrease to initiate irrigation, while increased in a lower rate of irrigation. The ascobarto peroxidase, catalase, superoxide dismutase and proline can be used as indicators of adaptability of hybrid melon 'Juazeiro' under water deficit.
V.G. Dias; A.F. Dutra; A.R.F. Silva; W.F. Dutra; C.H.S.G. Meneses; A.S. Melo. Antioxidant Enzymes and Proline as Indicators of Stress in Genotype Melon Irrigated Under Rates ETo. II Inovagri International Meeting 2014, 3806 -3812.
AMA StyleV.G. Dias, A.F. Dutra, A.R.F. Silva, W.F. Dutra, C.H.S.G. Meneses, A.S. Melo. Antioxidant Enzymes and Proline as Indicators of Stress in Genotype Melon Irrigated Under Rates ETo. II Inovagri International Meeting. 2014; ():3806-3812.
Chicago/Turabian StyleV.G. Dias; A.F. Dutra; A.R.F. Silva; W.F. Dutra; C.H.S.G. Meneses; A.S. Melo. 2014. "Antioxidant Enzymes and Proline as Indicators of Stress in Genotype Melon Irrigated Under Rates ETo." II Inovagri International Meeting , no. : 3806-3812.
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. This acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. It has been demonstrated that exopolysaccharides (EPS), which are produced by strain Pal5 of G. diazotrophicus, play an important role in plant infection. We have investigated the structure of the EPS, which was produced by a strain of Pal5 grown in liquid medium containing mannitol as the sole carbon source. The results reveal an EPS with Glc, Gal, Man in a molar ratio of 6:3:1, respectively. NMR spectroscopy and chemical derivatization have revealed that the EPS structure has 4-O-substituted units of β-glucose, 3-O-substituted units of β-galactose and 2-O-substituted units of α-mannose. Glucose and galactose units linked at C6 were also found. The structure proposed herein is different from EPS produced by other species of Gluconacetobacter published to date.
Rodrigo V. Serrato; Carlos Meneses; Marcia S. Vidal; Arquimedes Paixão de Santana-Filho; Marcello Iacomini; Guilherme L. Sassaki; José I. Baldani. Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5. Carbohydrate Polymers 2013, 98, 1153 -1159.
AMA StyleRodrigo V. Serrato, Carlos Meneses, Marcia S. Vidal, Arquimedes Paixão de Santana-Filho, Marcello Iacomini, Guilherme L. Sassaki, José I. Baldani. Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5. Carbohydrate Polymers. 2013; 98 (1):1153-1159.
Chicago/Turabian StyleRodrigo V. Serrato; Carlos Meneses; Marcia S. Vidal; Arquimedes Paixão de Santana-Filho; Marcello Iacomini; Guilherme L. Sassaki; José I. Baldani. 2013. "Structural studies of an exopolysaccharide produced by Gluconacetobacter diazotrophicus Pal5." Carbohydrate Polymers 98, no. 1: 1153-1159.
Gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. We showed that reactive oxygen species (ROS) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. The transcription of the pathogen-related-10 gene of the jasmonic acid (JA) pathway but not of the PR-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by G. diazotrophicus strain PAL5. Quantitative polymerase chain reaction analyses showed that, at early stages of colonization, the bacteria upregulated the transcript levels of ROS-detoxifying genes such as superoxide dismutase (SOD) and glutathione reductase (GR). To proof the role of ROS-scavenging enzymes in the colonization and interaction process, transposon insertion mutants of the SOD and GR genes of strain PAL5 were constructed. The SOD and GR mutants were unable to efficiently colonize the roots, indicated by the decrease of tightly root-associated bacterial cell counts and endophytic colonization and by fluorescence in situ hybridization analysis. Interestingly, the mutants did not induce the PR-10 of the JA-pathway, probably due to the inability of endophytic colonization. Thus, ROS-scavenging enzymes of G. diazotrophicus strain PAL5 play an important role in the endophytic colonization of rice plants.
Sylvia Alquéres; Carlos Meneses; Luc Rouws; Michael Rothballer; Ivo Baldani; Michael Schmid; Anton Hartmann. The Bacterial Superoxide Dismutase and Glutathione Reductase Are Crucial for Endophytic Colonization of Rice Roots by Gluconacetobacter diazotrophicus PAL5. Molecular Plant-Microbe Interactions® 2013, 26, 937 -945.
AMA StyleSylvia Alquéres, Carlos Meneses, Luc Rouws, Michael Rothballer, Ivo Baldani, Michael Schmid, Anton Hartmann. The Bacterial Superoxide Dismutase and Glutathione Reductase Are Crucial for Endophytic Colonization of Rice Roots by Gluconacetobacter diazotrophicus PAL5. Molecular Plant-Microbe Interactions®. 2013; 26 (8):937-945.
Chicago/Turabian StyleSylvia Alquéres; Carlos Meneses; Luc Rouws; Michael Rothballer; Ivo Baldani; Michael Schmid; Anton Hartmann. 2013. "The Bacterial Superoxide Dismutase and Glutathione Reductase Are Crucial for Endophytic Colonization of Rice Roots by Gluconacetobacter diazotrophicus PAL5." Molecular Plant-Microbe Interactions® 26, no. 8: 937-945.
The genome of the endophytic diazotrophic bacterial species Gluconacetobacter diazotrophicus PAL5 (PAL5) revealed the presence of a gum gene cluster. In this study, the gumD gene homologue, which is predicted to be responsible for the first step in exopolysaccharide (EPS) production, was insertionally inactivated and the resultant mutant (MGD) was functionally studied. The mutant MGD presented normal growth and nitrogen (N2) fixation levels but did not produce EPS when grown on different carbon sources. MGD presented altered colony morphology on soft agar plates (0.3% agar) and was defective in biofilm formation on glass wool. Most interestingly, MGD was defective in rice root surface attachment and in root surface and endophytic colonization. Genetic complementation reverted all mutant phenotypes. Also, the addition of EPS purified from culture supernatants of the wild-type strain PAL5 to the mutant MGD was effective in partially restoring wild-type biofilm formation and plant colonization. These data provide strong evidence that the PAL5 gumD gene is involved in EPS biosynthesis and that EPS biosynthesis is required for biofilm formation and plant colonization. To our knowledge, this is the first report of a role of EPS in the endophytic colonization of graminaceous plants by a nitrogen-fixing bacterium.
Carlos Meneses; Luc F. M. Rouws; Jean L. Simões-Araújo; Marcia S. Vidal; José I. Baldani. Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus. Molecular Plant-Microbe Interactions® 2011, 24, 1448 -1458.
AMA StyleCarlos Meneses, Luc F. M. Rouws, Jean L. Simões-Araújo, Marcia S. Vidal, José I. Baldani. Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus. Molecular Plant-Microbe Interactions®. 2011; 24 (12):1448-1458.
Chicago/Turabian StyleCarlos Meneses; Luc F. M. Rouws; Jean L. Simões-Araújo; Marcia S. Vidal; José I. Baldani. 2011. "Exopolysaccharide Production Is Required for Biofilm Formation and Plant Colonization by the Nitrogen-Fixing Endophyte Gluconacetobacter diazotrophicus." Molecular Plant-Microbe Interactions® 24, no. 12: 1448-1458.