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Dr. Marika Massaro is a full-time researcher at the Institute of Clinical Physiology (IFC) of the Italian National Research Council (CNR). She holds a BSc in Biological Sciences (cum laude) and a Ph.D. in Cellular and Molecular Pathology, with a specialization in Human Nutrition and Food Science (cum Laude). Her research interests are as follows: nutri- and pharmaco-genomics applied to obesity and cardiovascular disease. Her main activities are focused on the analysis of the anti-atherogenic and anti-inflammatory properties of marine omega-3 polyunsaturated fatty acids and plant food bioactives, and in the study of unexplored anti-inflammatory and anti-atherogenic properties of new and old cardiovascular drugs in the field of vascular biology. In recent years, her research interests have expanded to include the field of sports nutrition, community nutrition, the analyses of the heterogeneity in responsiveness to plant food bioactives, and the evaluation of dietary habits in the general population and in individuals at risk of different chronic diseases.
Cardiometabolic disorders are among the leading causes of mortality in the human population. Dietary polyphenols exert beneficial effects on cardiometabolic health in humans. Molecular mechanisms, however, are not completely understood. Aiming to conduct in-depth integrative bioinformatic analyses to elucidate molecular mechanisms underlying the protective effects of polyphenols on cardiometabolic health, we first conducted a systematic literature search to identify human intervention studies with polyphenols that demonstrate improvement of cardiometabolic risk factors in parallel with significant nutrigenomic effects. Applying the predefined inclusion criteria, we identified 58 differentially expressed genes at mRNA level and 5 miRNAs, analyzed in peripheral blood cells with RT-PCR methods. Subsequent integrative bioinformatic analyses demonstrated that polyphenols modulate genes that are mainly involved in the processes such as inflammation, lipid metabolism, and endothelial function. We also identified 37 transcription factors that are involved in the regulation of polyphenol modulated genes, including RELA/NFKB1, STAT1, JUN, or SIRT1. Integrative bioinformatic analysis of mRNA and miRNA-target pathways demonstrated several common enriched pathways that include MAPK signaling pathway, TNF signaling pathway, PI3K-Akt signaling pathway, focal adhesion, or PPAR signaling pathway. These bioinformatic analyses represent a valuable source of information for the identification of molecular mechanisms underlying the beneficial health effects of polyphenols and potential target genes for future nutrigenetic studies.
Tatjana Ruskovska; Irena Budić-Leto; Karla Corral-Jara; Vladimir Ajdžanović; Anna Arola-Arnal; Francisca Bravo; Georgia-Eirini Deligiannidou; Jaroslav Havlik; Milkica Janeva; Elena Kistanova; Christos Kontogiorgis; Irena Krga; Marika Massaro; Marko Miler; Verica Milosevic; Christine Morand; Egeria Scoditti; Manuel Suárez; David Vauzour; Dragan Milenkovic. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. Nutrients 2021, 13, 2326 .
AMA StyleTatjana Ruskovska, Irena Budić-Leto, Karla Corral-Jara, Vladimir Ajdžanović, Anna Arola-Arnal, Francisca Bravo, Georgia-Eirini Deligiannidou, Jaroslav Havlik, Milkica Janeva, Elena Kistanova, Christos Kontogiorgis, Irena Krga, Marika Massaro, Marko Miler, Verica Milosevic, Christine Morand, Egeria Scoditti, Manuel Suárez, David Vauzour, Dragan Milenkovic. Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies. Nutrients. 2021; 13 (7):2326.
Chicago/Turabian StyleTatjana Ruskovska; Irena Budić-Leto; Karla Corral-Jara; Vladimir Ajdžanović; Anna Arola-Arnal; Francisca Bravo; Georgia-Eirini Deligiannidou; Jaroslav Havlik; Milkica Janeva; Elena Kistanova; Christos Kontogiorgis; Irena Krga; Marika Massaro; Marko Miler; Verica Milosevic; Christine Morand; Egeria Scoditti; Manuel Suárez; David Vauzour; Dragan Milenkovic. 2021. "Systematic Bioinformatic Analyses of Nutrigenomic Modifications by Polyphenols Associated with Cardiometabolic Health in Humans—Evidence from Targeted Nutrigenomic Studies." Nutrients 13, no. 7: 2326.
Blood-derived concentrated growth factors (CGFs) represent a novel autologous biomaterial with promising applications in regenerative medicine. Angiogenesis is a key factor in tissue regeneration, but the role played by CGFs in vessel formation is not clear. The purpose of this study was to characterize the angiogenic properties of CGFs by evaluating the effects of its soluble factors and cellular components on the neovascularization in an in vitro model of angiogenesis. CGF clots were cultured for 14 days in cell culture medium; after that, CGF-conditioned medium (CGF-CM) was collected, and soluble factors and cellular components were separated and characterized. CGF-soluble factors, such as growth factors (VEGF and TGF-β1) and matrix metalloproteinases (MMP-2 and -9), were assessed by ELISA. Angiogenic properties of CGF-soluble factors were analyzed by stimulating human cultured endothelial cells with increasing concentrations (1%, 5%, 10%, or 20%) of CGF-CM, and their effect on cell migration and tubule-like formation was assessed by wound healing and Matrigel assay, respectively. The expression of endothelial angiogenic mediators was determined using qRT-PCR and ELISA assays. CGF-derived cells were characterized by immunostaining, qRT-PCR and Matrigel assay. We found that CGF-CM, consisting of essential pro-angiogenic factors, such as VEGF, TGF-β1, MMP-9, and MMP-2, promoted endothelial cell migration; tubule structure formation; and endothelial expression of multiple angiogenic mediators, including growth factors, chemokines, and metalloproteinases. Moreover, we discovered that CGF-derived cells exhibited features such as endothelial progenitor cells, since they expressed the CD34 stem cell marker and endothelial markers and participated in the neo-angiogenic process. In conclusion, our results suggest that CGFs are able to promote endothelial angiogenesis through their soluble and cellular components and that CGFs can be used as a biomaterial for therapeutic vasculogenesis in the field of tissue regeneration.
Nadia Calabriso; Eleonora Stanca; Alessio Rochira; Fabrizio Damiano; Laura Giannotti; Benedetta Di Chiara Stanca; Marika Massaro; Egeria Scoditti; Christian Demitri; Paola Nitti; Andrea Palermo; Luisa Siculella; Maria Carluccio. Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components. Pharmaceutics 2021, 13, 635 .
AMA StyleNadia Calabriso, Eleonora Stanca, Alessio Rochira, Fabrizio Damiano, Laura Giannotti, Benedetta Di Chiara Stanca, Marika Massaro, Egeria Scoditti, Christian Demitri, Paola Nitti, Andrea Palermo, Luisa Siculella, Maria Carluccio. Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components. Pharmaceutics. 2021; 13 (5):635.
Chicago/Turabian StyleNadia Calabriso; Eleonora Stanca; Alessio Rochira; Fabrizio Damiano; Laura Giannotti; Benedetta Di Chiara Stanca; Marika Massaro; Egeria Scoditti; Christian Demitri; Paola Nitti; Andrea Palermo; Luisa Siculella; Maria Carluccio. 2021. "Angiogenic Properties of Concentrated Growth Factors (CGFs): The Role of Soluble Factors and Cellular Components." Pharmaceutics 13, no. 5: 635.
Immunity is the consequence of a complex interaction between organs and the environment. It is mediated the interaction of several genes, receptors, molecules, hormones, cytokines, antibodies, antigens, and inflammatory mediators which in turn relate and influence the psychological health. The immune system response of heavily trained athletes resembles an even more complex conditions being theorized to follow a J or S shape dynamics at times. High training loads modify the immune response elevating the biological markers of immunity and the body susceptibility to infections. Heavy training and/or training in a cold environment increase the athletes’ risk to develop Upper Respiratory Tract Infections (URTIs). Therefore, athletes, who are considered healthier than the normal population, are in fact more prone to infections of the respiratory tract, due to lowering of the immune system in the time frames subsequent heavy training sessions. In this revision we will review the behavioral intervention, including nutritional approaches, useful to minimize the “open window” effect on infection and how to cope with stressors and boost the immune system in athletes.
Antonio Cicchella; Claudio Stefanelli; Marika Massaro. Upper Respiratory Tract Infections in Sport and the Immune System Response. A Review. Biology 2021, 10, 362 .
AMA StyleAntonio Cicchella, Claudio Stefanelli, Marika Massaro. Upper Respiratory Tract Infections in Sport and the Immune System Response. A Review. Biology. 2021; 10 (5):362.
Chicago/Turabian StyleAntonio Cicchella; Claudio Stefanelli; Marika Massaro. 2021. "Upper Respiratory Tract Infections in Sport and the Immune System Response. A Review." Biology 10, no. 5: 362.
The Mediterranean diet (MD) has been sponsored worldwide as a healthy and sustainable diet. Our aim was to update and compare MD adherence and food choices across several Southern European countries: Spain (SP), Portugal (PT), Italy (IT), Greece (GR), and Cyprus (CY) (MED, Mediterranean), and Bulgaria (BG) and the Republic of North Macedonia (NMK) (non-MED, non-Mediterranean). Participants (N = 3145, ≥18 y) completed a survey (MeDiWeB) with sociodemographic, anthropometric, and food questions (14-item Mediterranean Diet Adherence Screener, 14-MEDAS). The MED and non-MED populations showed moderate (7.08 ± 1.96) and weak (5.58 ± 1.82) MD adherence, respectively, with significant yet small differences across countries (SP > PT > GR > IT > CY > BG > NMK, p-value < 0.001). The MED participants scored higher than the non-MED ones for most of the Mediterranean-typical foods, with the greatest differences found for olive oil (OO) and white meat preference. In most countries, ≥70% of the participants reported quantities of red meat, butter, sweet drinks, and desserts below the recommended cutoff points, whereas <50% achieved the targets for plant-based foods, OO, fish, and wine. Being a woman and increasing age were associated with superior adherence (p-value < 0.001), but differences were rather small. Our results suggest that the campaigns carried out to support and reinforce the MD and to promote plant-based foods have limited success across Southern Europe, and that more hard-hitting strategies are needed.
Stefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients 2021, 13, 1432 .
AMA StyleStefano Quarta, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Rui Jorge, Vanda Andrade, Elena Philippou, Constantinos Zisimou, Viktorija Maksimova, Katarina Smilkov, Darinka Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Deligiannidou, Christos Kontogiorgis, Julio Sánchez-Meca, Paula Pinto, María-Teresa García-Conesa. Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations? Nutrients. 2021; 13 (5):1432.
Chicago/Turabian StyleStefano Quarta; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Rui Jorge; Vanda Andrade; Elena Philippou; Constantinos Zisimou; Viktorija Maksimova; Katarina Smilkov; Darinka Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Deligiannidou; Christos Kontogiorgis; Julio Sánchez-Meca; Paula Pinto; María-Teresa García-Conesa. 2021. "Persistent Moderate-to-Weak Mediterranean Diet Adherence and Low Scoring for Plant-Based Foods across Several Southern European Countries: Are We Overlooking the Mediterranean Diet Recommendations?" Nutrients 13, no. 5: 1432.
The Mediterranean diet (MD) and other lifestyle characteristics have been associated with well-being, a broad multiparameter concept that includes individual’s subjective assessment of their own well-being (SWB). Some studies have suggested that diet influences SWB, thus, this work aimed to add novel information on the association of MD and SWB in a sample of Portuguese adults. Data on sociodemographic, economic, lifestyle, diet, and SWB were collected through a self-filled online questionnaire. MD adherence was assessed by the Mediterranean Diet Adherence Screener (MEDAS) score ]. Results showed a moderate adherence to the MD in 490 Portuguese adults (mean MEDAS of 7.4 ± 2.1). A higher MD adherence was found to be significantly positively associated with women, employed individuals, a higher number of meals per day, and those with frequent contact with nature (p-value < 0.0025, using Bonferroni adjustment). As a novelty, this study divided the participants into low SWB, medium SWB, and medium to high SWB profiles (3.9 ± 1.0; 6.2 ± 1.0; 8.2 ± 1.3, respectively; p-value < 0.05), which reported significantly increasing MEDAS scores (6.5 ± 2.1; 7.3 ± 2.1; 7.8 ± 1.9; respectively, p-value < 0.05).
Vanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients 2020, 12, 3837 .
AMA StyleVanda Andrade, Rui Jorge, María-Teresa García-Conesa, Elena Philippou, Marika Massaro, Mihail Chervenkov, Teodora Ivanova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults. Nutrients. 2020; 12 (12):3837.
Chicago/Turabian StyleVanda Andrade; Rui Jorge; María-Teresa García-Conesa; Elena Philippou; Marika Massaro; Mihail Chervenkov; Teodora Ivanova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Mediterranean Diet Adherence and Subjective Well-Being in a Sample of Portuguese Adults." Nutrients 12, no. 12: 3837.
This study provides comprehensive validation of the 14-item Mediterranean Diet Adherence Screener (14-MEDAS) in an adult population from Greece (GR), Portugal (PT), Italy (IT), Spain (SP), Cyprus (CY), Republic of North Macedonia (NMK), and Bulgaria (BG). A moderate association between the 14-MEDAS and the reference food diary was estimated for the entire population (Pearson r = 0.573, p-value < 0.001; Intraclass Correlation Coefficient (ICC) = 0.692, p-value < 0.001) with the strongest correlation found in GR, followed by PT, IT, SP, and CY. These results were supported by kappa statistics in GR, PT, IT, and SP with ≥50% of food items exhibiting a fair or better agreement. Bland–Altman analyses showed an overestimation of the 14-MEDAS score in the whole population (0.79 ± 1.81, 95%Confidence Interval (CI) 0.61, 0.96), but this value was variable across countries, with GR, NMK, and BG exhibiting the lowest bias. Taking all analyses together, the validation achieved slightly better results in the Mediterranean countries but a definitive validation ranking order was not evident. Considering growing evidence of the shift from Mediterranean Diet (MD) adherence and of the importance of culture in making food choices it is crucial that we further improve validation protocols with specific applications to compare MD adherence across countries.
María-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients 2020, 12, 2960 .
AMA StyleMaría-Teresa García-Conesa, Elena Philippou, Christos Pafilas, Marika Massaro, Stefano Quarta, Vanda Andrade, Rui Jorge, Mihail Chervenkov, Teodora Ivanova, Dessislava Dimitrova, Viktorija Maksimova, Katarina Smilkov, Darinka Gjorgieva Ackova, Lence Miloseva, Tatjana Ruskovska, Georgia Eirini Deligiannidou, Christos A. Kontogiorgis, Paula Pinto. Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region. Nutrients. 2020; 12 (10):2960.
Chicago/Turabian StyleMaría-Teresa García-Conesa; Elena Philippou; Christos Pafilas; Marika Massaro; Stefano Quarta; Vanda Andrade; Rui Jorge; Mihail Chervenkov; Teodora Ivanova; Dessislava Dimitrova; Viktorija Maksimova; Katarina Smilkov; Darinka Gjorgieva Ackova; Lence Miloseva; Tatjana Ruskovska; Georgia Eirini Deligiannidou; Christos A. Kontogiorgis; Paula Pinto. 2020. "Exploring the Validity of the 14-Item Mediterranean Diet Adherence Screener (MEDAS): A Cross-National Study in Seven European Countries around the Mediterranean Region." Nutrients 12, no. 10: 2960.
The increasing access to antihypertensive medications has improved longevity and quality of life in hypertensive patients. Nevertheless, hypertension still remains a major risk factor for stroke and myocardial infarction, suggesting the need to implement management of pre- and hypertensive patients. In addition to antihypertensive medications, lifestyle changes, including healthier dietary patterns, such as the Dietary Approaches to Stop Hypertension (DASH) and the Mediterranean diet, have been shown to favorably affect blood pressure and are now recommended as integrative tools in hypertension management. An analysis of the effects of nutritional components of the Mediterranean diet(s) on blood pressure has therefore become mandatory. After a literature review of the impact of Mediterranean diet(s) on cardiovascular risk factors, we here analyze the effects of olive oil and its major components on blood pressure in healthy and cardiovascular disease individuals and examine underlying mechanisms of action. Both experimental and human studies agree in showing anti-hypertensive effects of olive oil. We conclude that due to its high oleic acid and antioxidant polyphenol content, the consumption of olive oil may be advised as the optimal fat choice in the management protocols for hypertension in both healthy and cardiovascular disease patients.
Marika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Nadia Calabriso; Giuseppe Santarpino; Tiziano Verri; Raffaele De Caterina. Effects of Olive Oil on Blood Pressure: Epidemiological, Clinical, and Mechanistic Evidence. Nutrients 2020, 12, 1548 .
AMA StyleMarika Massaro, Egeria Scoditti, Maria Annunziata Carluccio, Nadia Calabriso, Giuseppe Santarpino, Tiziano Verri, Raffaele De Caterina. Effects of Olive Oil on Blood Pressure: Epidemiological, Clinical, and Mechanistic Evidence. Nutrients. 2020; 12 (6):1548.
Chicago/Turabian StyleMarika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Nadia Calabriso; Giuseppe Santarpino; Tiziano Verri; Raffaele De Caterina. 2020. "Effects of Olive Oil on Blood Pressure: Epidemiological, Clinical, and Mechanistic Evidence." Nutrients 12, no. 6: 1548.
Inflammation of the adipose tissue plays an important role in the development of several chronic diseases associated with obesity. Polyphenols of extra virgin olive oil (EVOO), such as the secoiridoids oleocanthal (OC) and oleacein (OA), have many nutraceutical proprieties. However, their roles in obesity-associated adipocyte inflammation, the NF-κB pathway and related sub-networks have not been fully elucidated. Here, we investigated impact of OC and OA on the activation of NF-κB and the expression of molecules associated with inflammatory and dysmetabolic responses. To this aim, fully differentiated Simpson-Golabi-Behmel syndrome (SGBS) adipocytes were pre-treated with OC or OA before stimulation with TNF-α. EVOO polyphenols significantly reduced the expression of genes implicated in adipocyte inflammation (IL-1β, COX-2), angiogenesis (VEGF/KDR, MMP-2), oxidative stress (NADPH oxidase), antioxidant enzymes (SOD and GPX), leukocytes chemotaxis and infiltration (MCP-1, CXCL-10, MCS-F), and improved the expression of the anti-inflammatory/metabolic effector PPARγ. Accordingly, miR-155-5p, miR-34a-5p and let-7c-5p, tightly connected with the NF-κB pathway, were deregulated by TNF-α in both cells and exosomes. The miRNA modulation and NF-κB activation by TNF-α was significantly counteracted by EVOO polyphenols. Computational studies suggested a potential direct interaction between OC and NF-κB at the basis of its activity. This study demonstrates that OC and OA counteract adipocyte inflammation attenuating NF-κB activation. Therefore, these compounds could be novel dietary tools for the prevention of inflammatory diseases associated with obesity.
Sara Carpi; Egeria Scoditti; Marika Massaro; Beatrice Polini; Clementina Manera; Maria Digiacomo; Jasmine Esposito Salsano; Giulio Poli; Tiziano Tuccinardi; Stefano Doccini; Filippo Maria Santorelli; Maria Annunziata Carluccio; Marco Macchia; Martin Wabitsch; Raffaele De Caterina; Paola Nieri. The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation. Nutrients 2019, 11, 2855 .
AMA StyleSara Carpi, Egeria Scoditti, Marika Massaro, Beatrice Polini, Clementina Manera, Maria Digiacomo, Jasmine Esposito Salsano, Giulio Poli, Tiziano Tuccinardi, Stefano Doccini, Filippo Maria Santorelli, Maria Annunziata Carluccio, Marco Macchia, Martin Wabitsch, Raffaele De Caterina, Paola Nieri. The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation. Nutrients. 2019; 11 (12):2855.
Chicago/Turabian StyleSara Carpi; Egeria Scoditti; Marika Massaro; Beatrice Polini; Clementina Manera; Maria Digiacomo; Jasmine Esposito Salsano; Giulio Poli; Tiziano Tuccinardi; Stefano Doccini; Filippo Maria Santorelli; Maria Annunziata Carluccio; Marco Macchia; Martin Wabitsch; Raffaele De Caterina; Paola Nieri. 2019. "The Extra-Virgin Olive Oil Polyphenols Oleocanthal and Oleacein Counteract Inflammation-Related Gene and miRNA Expression in Adipocytes by Attenuating NF-κB Activation." Nutrients 11, no. 12: 2855.
The aim of the study was to evaluate the vascular health properties of extracts from biofortified bread, obtained by adding different durum wheat milling by-products rich in phenolic compounds, by analyzing their effects on overwhelming inflammatory response in endothelial cells and monocytes, two main players of atherogenesis. Human umbilical vein endothelial cells or U937 monocytes were incubated with increasing concentrations (1, 5, 10 μg/mL) of biofortified bread polyphenol extracts or corresponding pure phenolic acids before stimulation with lipopolysaccharide (LPS). We analyzed the endothelial-monocyte adhesion and related endothelial adhesion molecules. The expression of chemokines and pro-inflammatory cytokines was also measured in LPS-stimulated endothelial cells and monocytes as well as intracellular oxidative stress. Biofortified bread extracts inhibited monocyte adhesion to LPS-stimulated endothelial cells, in a concentration-dependent manner by reducing mainly endothelial VCAM-1 expression. Phenolic acid extracts contained in 10 mg biofortified bread downregulated the LPS-induced expression of chemokines MCP-1, M-CSF, and CXCL-10 as well as pro-inflammatory cytokines TNF-α and IL-1β, in endothelial cells and monocytes, with CXCL-10 as the most reduced inflammatory mediator. Among phenolic acids of biofortified bread, ferulic, sinapic, and p-coumaric acids significantly inhibited the LPS-stimulated CXCL-10 expression in vascular cells. The reduced pro-inflammatory response was related to a slightly but significant reduction of intracellular oxidative stress. Our findings suggest the bread biofortified with selected durum wheat milling by-products as a source of phenolic acids with multiple anti-inflammatory and anti-atherosclerotic properties, which could help to counteract or prevent inflammatory vascular diseases.
Nadia Calabriso; Marika Massaro; Egeria Scoditti; Antonella Pasqualone; Barbara Laddomada; Maria Annunziata Carluccio. Phenolic extracts from whole wheat biofortified bread dampen overwhelming inflammatory response in human endothelial cells and monocytes: major role of VCAM-1 and CXCL-10. European Journal of Nutrition 2019, 59, 2603 -2615.
AMA StyleNadia Calabriso, Marika Massaro, Egeria Scoditti, Antonella Pasqualone, Barbara Laddomada, Maria Annunziata Carluccio. Phenolic extracts from whole wheat biofortified bread dampen overwhelming inflammatory response in human endothelial cells and monocytes: major role of VCAM-1 and CXCL-10. European Journal of Nutrition. 2019; 59 (6):2603-2615.
Chicago/Turabian StyleNadia Calabriso; Marika Massaro; Egeria Scoditti; Antonella Pasqualone; Barbara Laddomada; Maria Annunziata Carluccio. 2019. "Phenolic extracts from whole wheat biofortified bread dampen overwhelming inflammatory response in human endothelial cells and monocytes: major role of VCAM-1 and CXCL-10." European Journal of Nutrition 59, no. 6: 2603-2615.
Chronic inflammation of the adipose tissue (AT) is a major contributor to obesity-associated cardiometabolic complications. The olive oil polyphenol hydroxytyrosol (HT) contributes to Mediterranean diet cardiometabolic benefits through mechanisms still partially unknown. We investigated HT (1 and 10 μmol/L) effects on gene expression (mRNA and microRNA) related to inflammation induced by 10 ng/mL tumor necrosis factor (TNF)-α in human Simpson–Golabi–Behmel Syndrome (SGBS) adipocytes. At real-time PCR, HT significantly inhibited TNF-α-induced mRNA levels, of monocyte chemoattractant protein-1, C-X-C Motif Ligand-10, interleukin (IL)-1β, IL-6, vascular endothelial growth factor, plasminogen activator inhibitor-1, cyclooxygenase-2, macrophage colony-stimulating factor, matrix metalloproteinase-2, Cu/Zn superoxide dismutase-1, and glutathione peroxidase, as well as surface expression of intercellular adhesion molecule-1, and reverted the TNF-α-mediated inhibition of endothelial nitric oxide synthase, peroxisome proliferator-activated receptor coactivator-1α, and glucose transporter-4. We found similar effects in adipocytes stimulated by macrophage-conditioned media. Accordingly, HT significantly counteracted miR-155-5p, miR-34a-5p, and let-7c-5p expression in both cells and exosomes, and prevented NF-κB activation and production of reactive oxygen species. HT can therefore modulate adipocyte gene expression profile through mechanisms involving a reduction of oxidative stress and NF-κB inhibition. By such mechanisms, HT may blunt macrophage recruitment and improve AT inflammation, preventing the deregulation of pathways involved in obesity-related diseases.
Egeria Scoditti; Sara Carpi; Marika Massaro; Mariangela Pellegrino; Beatrice Polini; Maria Annunziata Carluccio; Martin Wabitsch; Tiziano Verri; Paola Nieri; Raffaele De Caterina. Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition. Nutrients 2019, 11, 2493 .
AMA StyleEgeria Scoditti, Sara Carpi, Marika Massaro, Mariangela Pellegrino, Beatrice Polini, Maria Annunziata Carluccio, Martin Wabitsch, Tiziano Verri, Paola Nieri, Raffaele De Caterina. Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition. Nutrients. 2019; 11 (10):2493.
Chicago/Turabian StyleEgeria Scoditti; Sara Carpi; Marika Massaro; Mariangela Pellegrino; Beatrice Polini; Maria Annunziata Carluccio; Martin Wabitsch; Tiziano Verri; Paola Nieri; Raffaele De Caterina. 2019. "Hydroxytyrosol Modulates Adipocyte Gene and miRNA Expression Under Inflammatory Condition." Nutrients 11, no. 10: 2493.
The evaluation of volatile organic compounds(VOCs) emitted by human body offers a unique tool to set up new non-invasive devices for early diagnosis and long-lasting monitoring of most human diseases. However, their cellular origin and metabolic fate have not been completely elucidated yet, thus limiting their clinical application. Endothelium acts as an interface between blood and surrounding tissues. As such, it adapts its physiology in response to different environmental modifications thus playing a role in the pathogenesis of many metabolic and inflammatory diseases. Since endothelium specifically reshapes its physiologic functions upon environmental changes the objective of this study was to evaluate if and how pro-inflammatory stimuli affect VOC metabolism in endothelial cell in culture. Gas chromatography with mass spectrometric detection was applied to profile VOCs in the headspace of cultured endothelial cells (EC) in the absence or presence of the pro-inflammatory stimulus lipopolysaccharide (LPS). We observed that, under resting conditions, EC affected the amount of 58 VOCs belonging to aldehyde, alkane and ketone families. Among these, LPS significantly altered the amount of 15 VOCs. ROC curves show a perfect performance (AUC = 1) for 10 metabolites including 1-butanol, 3-methyl-1-butanol and 2-ethyl-1-hexanol. The emission and uptake of the aforementioned VOCs disclose potential unexplored metabolic pathways for EC that deserve to be investigated. Overall, we identified new candidate VOC potentially exploitable, upon experimental confirm in in vivo model of disease, as potential biomarkers of sepsis and pro-inflammatory clinical settings.
V. Longo; A. Forleo; S. Capone; E. Scoditti; M. A. Carluccio; P. Siciliano; M. Massaro. In vitro profiling of endothelial volatile organic compounds under resting and pro-inflammatory conditions. Metabolomics 2019, 15, 132 .
AMA StyleV. Longo, A. Forleo, S. Capone, E. Scoditti, M. A. Carluccio, P. Siciliano, M. Massaro. In vitro profiling of endothelial volatile organic compounds under resting and pro-inflammatory conditions. Metabolomics. 2019; 15 (10):132.
Chicago/Turabian StyleV. Longo; A. Forleo; S. Capone; E. Scoditti; M. A. Carluccio; P. Siciliano; M. Massaro. 2019. "In vitro profiling of endothelial volatile organic compounds under resting and pro-inflammatory conditions." Metabolomics 15, no. 10: 132.
The quality of the study design and data reporting in human trials dealing with the inter-individual variability in response to the consumption of plant bioactives is, in general, low. There is a lack of recommendations supporting the scientific community on this topic. This study aimed at developing a quality index to assist the assessment of the reporting quality of intervention trials addressing the inter-individual variability in response to plant bioactive consumption. Recommendations for better designing and reporting studies were discussed. The selection of the parameters used for the development of the quality index was carried out in agreement with the scientific community through a survey. Parameters were defined, grouped into categories, and scored for different quality levels. The applicability of the scoring system was tested in terms of consistency and effort, and its validity was assessed by comparison with a simultaneous evaluation by experts’ criteria. The “POSITIVe quality index” included 11 reporting criteria grouped into four categories (Statistics, Reporting, Data presentation, and Individual data availability). It was supported by detailed definitions and guidance for their scoring. The quality index score was tested, and the index demonstrated to be valid, reliable, and responsive. The evaluation of the reporting quality of studies addressing inter-individual variability in response to plant bioactives highlighted the aspects requiring major improvements. Specific tools and recommendations favoring a complete and transparent reporting on inter-individual variability have been provided to support the scientific community on this field.
Marina Nikolic; Aleksandra Konic Ristic; Antonio González-Sarrías; Geoffrey Istas; Mireia Urpi-Sarda; Margherita Dall’Asta; Laurent-Emmanuel Monfoulet; Lieselotte Cloetens; Banu Bayram; Maria Rosaria Tumolo; Mihail Chervenkov; Egeria Scoditti; Marika Massaro; Noemi Tejera; Desislava Abadjieva; Karen Chambers; Irena Krga; Francisco A. Tomás-Barberán; Christine Morand; Rodrigo Feliciano; Rocío García-Villalba; Mar Garcia-Aloy; Pedro Mena. Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. European Journal of Nutrition 2019, 58, 49 -64.
AMA StyleMarina Nikolic, Aleksandra Konic Ristic, Antonio González-Sarrías, Geoffrey Istas, Mireia Urpi-Sarda, Margherita Dall’Asta, Laurent-Emmanuel Monfoulet, Lieselotte Cloetens, Banu Bayram, Maria Rosaria Tumolo, Mihail Chervenkov, Egeria Scoditti, Marika Massaro, Noemi Tejera, Desislava Abadjieva, Karen Chambers, Irena Krga, Francisco A. Tomás-Barberán, Christine Morand, Rodrigo Feliciano, Rocío García-Villalba, Mar Garcia-Aloy, Pedro Mena. Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations. European Journal of Nutrition. 2019; 58 (S2):49-64.
Chicago/Turabian StyleMarina Nikolic; Aleksandra Konic Ristic; Antonio González-Sarrías; Geoffrey Istas; Mireia Urpi-Sarda; Margherita Dall’Asta; Laurent-Emmanuel Monfoulet; Lieselotte Cloetens; Banu Bayram; Maria Rosaria Tumolo; Mihail Chervenkov; Egeria Scoditti; Marika Massaro; Noemi Tejera; Desislava Abadjieva; Karen Chambers; Irena Krga; Francisco A. Tomás-Barberán; Christine Morand; Rodrigo Feliciano; Rocío García-Villalba; Mar Garcia-Aloy; Pedro Mena. 2019. "Improving the reporting quality of intervention trials addressing the inter-individual variability in response to the consumption of plant bioactives: quality index and recommendations." European Journal of Nutrition 58, no. S2: 49-64.
Plant-based diets rich in bioactive compounds such as polyphenols have been shown to positively modulate the risk of cardiometabolic (CM) diseases. The inter-individual variability in the response to these bioactives may affect the findings. This systematic review aimed to summarize findings from existing randomized clinical trials (RCTs) evaluating the effect of hydroxycinnamic acids (HCAs) on markers of CM health in humans. Literature searches were performed in PubMed and the Web of Science. RCTs on acute and chronic supplementation of HCA-rich foods/extracts on CM biomarkers were included. Forty-four RCTs (21 acute and 23 chronic) met inclusion criteria. Comparisons were made between RCTs, including assessments based on population health status. Of the 44 RCTs, only seven performed analyses on a factor exploring inter-individual response to HCA consumption. Results demonstrated that health status is a potentially important effect modifier as RCTs with higher baseline cholesterol, blood pressure and glycaemia demonstrated greater overall effectiveness, which was also found in studies where specific subgroup analyses were performed. Thus, the effect of HCAs on CM risk factors may be greater in individuals at higher CM risk, although future studies in these populations are needed, including those on other potential determinants of inter-individual variability. PROSPERO, registration number CRD42016050790.
Daniela Martini; Laura Chiavaroli; Antonio González-Sarrías; Letizia Bresciani; Susana A. Palma-Duran; Margherita Dall’Asta; Georgia-Eirini Deligiannidou; Marika Massaro; Egeria Scoditti; Emilie Combet; Viktorija Maksimova; Mireia Urpi-Sarda; Christos A. Kontogiorgis; Cristina Andrés-Lacueva; Eileen R. Gibney; Daniele Del Rio; Christine Morand; Mar Garcia-Aloy; Ana Rodriguez-Mateos; Pedro Mena. Impact of Foods and Dietary Supplements Containing Hydroxycinnamic Acids on Cardiometabolic Biomarkers: A Systematic Review to Explore Inter-Individual Variability. Nutrients 2019, 11, 1805 .
AMA StyleDaniela Martini, Laura Chiavaroli, Antonio González-Sarrías, Letizia Bresciani, Susana A. Palma-Duran, Margherita Dall’Asta, Georgia-Eirini Deligiannidou, Marika Massaro, Egeria Scoditti, Emilie Combet, Viktorija Maksimova, Mireia Urpi-Sarda, Christos A. Kontogiorgis, Cristina Andrés-Lacueva, Eileen R. Gibney, Daniele Del Rio, Christine Morand, Mar Garcia-Aloy, Ana Rodriguez-Mateos, Pedro Mena. Impact of Foods and Dietary Supplements Containing Hydroxycinnamic Acids on Cardiometabolic Biomarkers: A Systematic Review to Explore Inter-Individual Variability. Nutrients. 2019; 11 (8):1805.
Chicago/Turabian StyleDaniela Martini; Laura Chiavaroli; Antonio González-Sarrías; Letizia Bresciani; Susana A. Palma-Duran; Margherita Dall’Asta; Georgia-Eirini Deligiannidou; Marika Massaro; Egeria Scoditti; Emilie Combet; Viktorija Maksimova; Mireia Urpi-Sarda; Christos A. Kontogiorgis; Cristina Andrés-Lacueva; Eileen R. Gibney; Daniele Del Rio; Christine Morand; Mar Garcia-Aloy; Ana Rodriguez-Mateos; Pedro Mena. 2019. "Impact of Foods and Dietary Supplements Containing Hydroxycinnamic Acids on Cardiometabolic Biomarkers: A Systematic Review to Explore Inter-Individual Variability." Nutrients 11, no. 8: 1805.
In recent years, the consumption of chocolate and, in particular, dark chocolate has been “rehabilitated” due to its high content of cocoa antioxidant polyphenols. Although it is recognized that regular exercise improves energy metabolism and muscle performance, excessive or unaccustomed exercise may induce cell damage and impair muscle function by triggering oxidative stress and tissue inflammation. The aim of this review was to revise the available data from literature on the effects of cocoa polyphenols on exercise-associated tissue damage and impairment of exercise performance. To this aim, PubMed and Web of Science databases were searched with the following keywords: “intervention studies”, “cocoa polyphenols”, “exercise training”, “inflammation”, “oxidative stress”, and “exercise performance”. We selected thirteen randomized clinical trials on cocoa ingestion that involved a total of 200 well-trained athletes. The retrieved data indicate that acute, sub-chronic, and chronic cocoa polyphenol intake may reduce exercise-induced oxidative stress but not inflammation, while mixed results are observed in terms of exercise performance and recovery. The interpretation of available results on the anti-oxidative and anti-inflammatory activities of cocoa polyphenols remains questionable, likely due to the variety of physiological networks involved. Further experimental studies are mandatory to clarify the role of cocoa polyphenol supplementation in exercise-mediated inflammation.
Marika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Antonia Kaltsatou; Antonio Cicchella. Effect of Cocoa Products and Its Polyphenolic Constituents on Exercise Performance and Exercise-Induced Muscle Damage and Inflammation: A Review of Clinical Trials. Nutrients 2019, 11, 1471 .
AMA StyleMarika Massaro, Egeria Scoditti, Maria Annunziata Carluccio, Antonia Kaltsatou, Antonio Cicchella. Effect of Cocoa Products and Its Polyphenolic Constituents on Exercise Performance and Exercise-Induced Muscle Damage and Inflammation: A Review of Clinical Trials. Nutrients. 2019; 11 (7):1471.
Chicago/Turabian StyleMarika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Antonia Kaltsatou; Antonio Cicchella. 2019. "Effect of Cocoa Products and Its Polyphenolic Constituents on Exercise Performance and Exercise-Induced Muscle Damage and Inflammation: A Review of Clinical Trials." Nutrients 11, no. 7: 1471.
Chronic obstructive pulmonary disease is one of the leading causes of morbidity and mortality worldwide and a growing healthcare problem. Identification of modifiable risk factors for prevention and treatment of COPD is urgent, and the scientific community has begun to pay close attention to diet as an integral part of COPD management, from prevention to treatment. This review summarizes the evidence from observational and clinical studies regarding the impact of nutrients and dietary patterns on lung function and COPD development, progression, and outcomes, with highlights on potential mechanisms of action. Several dietary options can be considered in terms of COPD prevention and/or progression. Although definitive data are lacking, the available scientific evidence indicates that some foods and nutrients, especially those nutraceuticals endowed with antioxidant and anti-inflammatory properties and when consumed in combinations in the form of balanced dietary patterns, are associated with better pulmonary function, less lung function decline, and reduced risk of COPD. Knowledge of dietary influences on COPD may provide health professionals with an evidence-based lifestyle approach to better counsel patients toward improved pulmonary health.
Egeria Scoditti; Marika Massaro; Sergio Garbarino; Domenico Maurizio Toraldo. Role of Diet in Chronic Obstructive Pulmonary Disease Prevention and Treatment. Nutrients 2019, 11, 1357 .
AMA StyleEgeria Scoditti, Marika Massaro, Sergio Garbarino, Domenico Maurizio Toraldo. Role of Diet in Chronic Obstructive Pulmonary Disease Prevention and Treatment. Nutrients. 2019; 11 (6):1357.
Chicago/Turabian StyleEgeria Scoditti; Marika Massaro; Sergio Garbarino; Domenico Maurizio Toraldo. 2019. "Role of Diet in Chronic Obstructive Pulmonary Disease Prevention and Treatment." Nutrients 11, no. 6: 1357.
Since the first successful launch of the Veterans Administration(VA) cooperative studies in the late 1960s, the increasing access to blood pressure lowering medications has significantly contributed to improving longevity and quality of life in hypertensive patients. Since then, insights into the pathogenesis of hypertension have shown a mechanistic role for reactive oxygen species (ROS) in all phases of disease progression, suggesting the potential utility of antioxidant therapies to counteract symptoms and, at the same time, treat a fundamental mechanism of the disease. Despite these progresses, hypertension still remains the main contributor to the global incidence of cardiovascular disease and the leading cause of morbidity and mortality worldwide. We here briefly review and update the role of ROS and ROS-dependent metalloproteinase activation in the maladaptive remodeling of the vascular wall in hypertension. Such understanding should provide new Potential sites of action for antioxidant therapies as an integrated therapeutic approach to hypertension and its consequences.
Marika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Raffaele De Caterina. Oxidative stress and vascular stiffness in hypertension: A renewed interest for antioxidant therapies? Vascular Pharmacology 2019, 116, 45 -50.
AMA StyleMarika Massaro, Egeria Scoditti, Maria Annunziata Carluccio, Raffaele De Caterina. Oxidative stress and vascular stiffness in hypertension: A renewed interest for antioxidant therapies? Vascular Pharmacology. 2019; 116 ():45-50.
Chicago/Turabian StyleMarika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Raffaele De Caterina. 2019. "Oxidative stress and vascular stiffness in hypertension: A renewed interest for antioxidant therapies?" Vascular Pharmacology 116, no. : 45-50.
The adipose tissue (AT) is an endocrine organ that produces adipocytokines (adipokines), able to influence metabolic homeostasis. In the conventional classification, there are two large AT depots, characterized by different paracrine activities: the subcutaneous AT, which would mostly produce cytokines with protective properties against cardiovascular disease; and the visceral AT, responsible for the secretion of cytokines with proinflammatory, prothrombotic, and proatherogenic effects. A third component, the epicardial AT (EAT) is now receiving increasing attention due to its unique anatomical and functional proximity to the myocardium and the coronary arteries. In rodents, the EAT protects the heart from exposure to high levels of free fatty acids, and provides energy to the myocardium under high metabolic demands. The observation that atherosclerotic plaques are more prevalent in regions of coronary arteries surrounded by the EAT, while they tend to be less present in segments penetrating the myocardium (the septal branches and segments under myocardial bridges), has led to the hypothesis of a possible role of the EAT in promoting the development of atherosclerosis through endocrine and paracrine effects, in addition to the role of biomechanical forces affecting transendothelial lipid permeability into the intima. In this article, we review the clinical and molecular evidence linking the EAT and coronary artery disease through a systematic review of the literature. We, here, discuss current diagnostic techniques in evaluating the interaction between EAT and the onset of coronary artery disease and ischaemic heart disease. Finally, we review current knowledge on the underlying mechanisms by which the EAT may affect coronary atherosclerosis, and potential clinical implications of this interaction, making the EAT an attractive target for new therapeutics in cardiovascular disease.
Rosalinda Madonna; Marika Massaro; Egeria Scoditti; Irene Pescetelli; Raffaele De Caterina. The epicardial adipose tissue and the coronary arteries: dangerous liaisons. Cardiovascular Research 2019, 115, 1013 -1025.
AMA StyleRosalinda Madonna, Marika Massaro, Egeria Scoditti, Irene Pescetelli, Raffaele De Caterina. The epicardial adipose tissue and the coronary arteries: dangerous liaisons. Cardiovascular Research. 2019; 115 (6):1013-1025.
Chicago/Turabian StyleRosalinda Madonna; Marika Massaro; Egeria Scoditti; Irene Pescetelli; Raffaele De Caterina. 2019. "The epicardial adipose tissue and the coronary arteries: dangerous liaisons." Cardiovascular Research 115, no. 6: 1013-1025.
Early atherosclerosis features functional and structural changes in the endothelial barrier function that affect the traffic of molecules and solutes between the vessel lumen and the vascular wall. Such changes are mechanistically related to the development of atherosclerosis. Proatherogenic stimuli and cardiovascular risk factors, such as dyslipidaemias, diabetes, obesity, and smoking, all increase endothelial permeability sharing a common signalling denominator: an imbalance in the production/disposal of reactive oxygen species (ROS), broadly termed oxidative stress. Mostly as a consequence of the activation of enzymatic systems leading to ROS overproduction, proatherogenic factors lead to a pro-inflammatory status that translates in changes in gene expression and functional rearrangements, including changes in the transendothelial transport of molecules, leading to the deposition of low-density lipoproteins (LDL) and the subsequent infiltration of circulating leucocytes in the intima. In this review, we focus on such early changes in atherogenesis and on the concept that proatherogenic stimuli and risk factors for cardiovascular disease, by altering the endothelial barrier properties, co-ordinately trigger the accumulation of LDL in the intima and ultimately plaque formation.
Santa Mundi; Marika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Victor W M van Hinsbergh; Luisa Iruela-Arispe; Raffaele De Caterina. Endothelial permeability, LDL deposition, and cardiovascular risk factors—a review. Cardiovascular Research 2017, 114, 35 -52.
AMA StyleSanta Mundi, Marika Massaro, Egeria Scoditti, Maria Annunziata Carluccio, Victor W M van Hinsbergh, Luisa Iruela-Arispe, Raffaele De Caterina. Endothelial permeability, LDL deposition, and cardiovascular risk factors—a review. Cardiovascular Research. 2017; 114 (1):35-52.
Chicago/Turabian StyleSanta Mundi; Marika Massaro; Egeria Scoditti; Maria Annunziata Carluccio; Victor W M van Hinsbergh; Luisa Iruela-Arispe; Raffaele De Caterina. 2017. "Endothelial permeability, LDL deposition, and cardiovascular risk factors—a review." Cardiovascular Research 114, no. 1: 35-52.
Leukemia is a group of cancers that usually begin in the bone marrow and result in high numbers of abnormal and dysfunctional white blood cells. Many studies were carried out to investigate metabolism of these cells. Metabolome analysis has been successfully applied to leukemia disease and emerged as a powerful tool for obtaining information about the biological processes that occur in organisms, and as a useful platform for discovering new clinical biomarkers and make diagnosis of disease using different biofluids. Whatever has not been investigated in leukemic cells is volatile metabolic signature that in recent literature is called “volatilome”. Volatile organic compounds (VOCs) from the headspace of cultured THP1 cells and normal human peripheral blood mononuclear cells, were collected by headspace solid-phase microextraction (HS-SPME) and analyzed by gas chromatography combined with mass spectrometry (GC–MS), thus defining a volatile metabolomics signature. Styrene, cyclohexanol, cyclohexanone, 1-hexanol-2-ethyl, cyclohexane, 1,1’-(1,2-dimethyl-1,2-ethanediyl)bis-, benzene, 1,3-bis(1,1-dimethylethyl)- were present in higher amount in cultured THP1 cell than in PBMC, while 2-butanone has an opposite trend. Cell stimulation with lipopolysaccharide affected normal cells, but not leukemic cells. The establishment of the volatile fingerprint of THP1 cell lines presents a powerful approach to find endogenous VOCs that could be used to improve the diagnostic tools and explore the associated metabolomic pathways.
A. Forleo; Simonetta Capone; V. Longo; F. Casino; Antonio Vincenzo Radogna; P. Siciliano; Marika Massaro; Egeria Scoditti; Nadia Calabriso; Maria Annunziata Carluccio. Evaluation of the Volatile Organic Compounds Released from Peripheral Blood Mononuclear Cells and THP1 Cells Under Normal and Proinflammatory Conditions. Lecture Notes in Electrical Engineering 2017, 269 -277.
AMA StyleA. Forleo, Simonetta Capone, V. Longo, F. Casino, Antonio Vincenzo Radogna, P. Siciliano, Marika Massaro, Egeria Scoditti, Nadia Calabriso, Maria Annunziata Carluccio. Evaluation of the Volatile Organic Compounds Released from Peripheral Blood Mononuclear Cells and THP1 Cells Under Normal and Proinflammatory Conditions. Lecture Notes in Electrical Engineering. 2017; ():269-277.
Chicago/Turabian StyleA. Forleo; Simonetta Capone; V. Longo; F. Casino; Antonio Vincenzo Radogna; P. Siciliano; Marika Massaro; Egeria Scoditti; Nadia Calabriso; Maria Annunziata Carluccio. 2017. "Evaluation of the Volatile Organic Compounds Released from Peripheral Blood Mononuclear Cells and THP1 Cells Under Normal and Proinflammatory Conditions." Lecture Notes in Electrical Engineering , no. : 269-277.
Several randomized controlled trials (RCTs) and meta-analyses support the benefits of flavanols on cardiometabolic health, but the factors affecting variability in the responses to these compounds have not been properly assessed. The objectives of this meta-analysis were to systematically collect the RCTs-based-evidence of the effects of flavanol-containing tea, cocoa and apple products on selected biomarkers of cardiometabolic risk and to explore the influence of various factors on the variability in the responses to the consumption of these products. A total of 120 RCTs were selected. Despite a high heterogeneity, the intake of the flavanol-containing products was associated using a random model with changes (reported as standardized difference in means (SDM)) in body mass index (−0.15, p < 0.001), waist circumference (−0.29, p < 0.001), total-cholesterol (−0.21, p < 0.001), LDL-cholesterol (−0.23, p < 0.001), and triacylglycerides (−0.11, p = 0.027), and with an increase of HDL-cholesterol (0.15, p = 0.005). Through subgroup analyses, we showed the influence of baseline-BMI, sex, source/form of administration, medication and country of investigation on some of the outcome measures and suggest that flavanols may be more effective in specific subgroups such as those with a BMI ≥ 25.0 kg/m2, non-medicated individuals or by specifically using tea products. This meta-analysis provides the first robust evidence of the effects induced by the consumption of flavanol-containing tea, cocoa and apple products on weight and lipid biomarkers and shows the influence of various factors that can affect their bioefficacy in humans. Of note, some of these effects are quantitatively comparable to those produced by drugs, life-style changes or other natural products. Further, RCTs in well-characterized populations are required to fully comprehend the factors affecting inter-individual responses to flavanol and thereby improve flavanols efficacy in the prevention of cardiometabolic disorders.
Antonio González-Sarrías; Emilie Combet; Paula Pinto; Pedro Mena; Margherita Dall’Asta; Mar Garcia-Aloy; Ana Rodríguez-Mateos; Eileen R. Gibney; Julie Dumont; Marika Massaro; Julio Sánchez-Meca; Christine Morand; María-Teresa García-Conesa. A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy. Nutrients 2017, 9, 746 .
AMA StyleAntonio González-Sarrías, Emilie Combet, Paula Pinto, Pedro Mena, Margherita Dall’Asta, Mar Garcia-Aloy, Ana Rodríguez-Mateos, Eileen R. Gibney, Julie Dumont, Marika Massaro, Julio Sánchez-Meca, Christine Morand, María-Teresa García-Conesa. A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy. Nutrients. 2017; 9 (7):746.
Chicago/Turabian StyleAntonio González-Sarrías; Emilie Combet; Paula Pinto; Pedro Mena; Margherita Dall’Asta; Mar Garcia-Aloy; Ana Rodríguez-Mateos; Eileen R. Gibney; Julie Dumont; Marika Massaro; Julio Sánchez-Meca; Christine Morand; María-Teresa García-Conesa. 2017. "A Systematic Review and Meta-Analysis of the Effects of Flavanol-Containing Tea, Cocoa and Apple Products on Body Composition and Blood Lipids: Exploring the Factors Responsible for Variability in Their Efficacy." Nutrients 9, no. 7: 746.