This page has only limited features, please log in for full access.

Dr. Michelangelo Pascale
National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), 70126 Bari, Italy

Basic Info


Research Keywords & Expertise

0 A
0 Argentina
0 carbon dioxide
0 Fusarium
0 maize

Fingerprints

A
Fusarium
maize
Argentina
carbon dioxide

Honors and Awards

The user has no records in this section


Career Timeline

The user has no records in this section.


Short Biography

The user biography is not available.
Following
Followers
Co Authors
The list of users this user is following is empty.
Following: 0 users

Feed

Journal article
Published: 14 December 2020 in Polymers
Reads 0
Downloads 0

Aptamers are single-stranded oligonucleotides selected by SELEX (Systematic Evolution of Ligands by EXponential Enrichment) able to discriminate target molecules with high affinity and specificity, even in the case of very closely related structures. Aptamers have been produced for several targets including small molecules like mycotoxins; however, the high affinity for their respective target molecules is a critical requirement. In the last decade, the screening through computational methods of aptamers for their affinity against specific targets has greatly increased and is becoming a commonly used procedure due to its convenience and low costs. This paper describes an in-silico approach for rapid screening of ten ssDNA aptamer sequences against fumonisin B1 (FB1, n = 3), aflatoxin B1 (AFB1, n = 2) and ochratoxin A (OTA, n = 5). Theoretical results were compared with those obtained by testing the same aptamers by fluorescent microscale thermophoresis and by magnetic beads assay for their binding affinity (KD) revealing a good agreement.

ACS Style

Fulvio Ciriaco; Vincenzo De Leo; Lucia Catucci; Michelangelo Pascale; Antonio F. Logrieco; Maria C. DeRosa; Annalisa De Girolamo. An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A. Polymers 2020, 12, 2983 .

AMA Style

Fulvio Ciriaco, Vincenzo De Leo, Lucia Catucci, Michelangelo Pascale, Antonio F. Logrieco, Maria C. DeRosa, Annalisa De Girolamo. An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A. Polymers. 2020; 12 (12):2983.

Chicago/Turabian Style

Fulvio Ciriaco; Vincenzo De Leo; Lucia Catucci; Michelangelo Pascale; Antonio F. Logrieco; Maria C. DeRosa; Annalisa De Girolamo. 2020. "An In-Silico Pipeline for Rapid Screening of DNA Aptamers against Mycotoxins: The Case-Study of Fumonisin B1, Aflatoxin B1 and Ochratoxin A." Polymers 12, no. 12: 2983.

Journal article
Published: 10 December 2020 in Toxins
Reads 0
Downloads 0

An analytical method for the simultaneous determination of trichothecenes—namely, nivalenol (NIV), deoxynivalenol (DON) and its acetylated derivatives (3- and 15-acetyl-DON), T-2 and HT-2 toxins—and zearalenone (ZEN) in wheat, wheat flour, and wheat crackers was validated through a collaborative study involving 15 participants from 10 countries. The validation study, performed within the M/520 standardization mandate of the European Commission, was carried out according to the IUPAC (International Union of Pure and Applied Chemistry) International Harmonized Protocol. The method was based on mycotoxin extraction from the homogenized sample material with a mixture of acetonitrile-water followed by purification and concentration on a solid phase extraction column. High-performance liquid chromatography coupled with tandem mass spectrometry was used for mycotoxin detection, using isotopically labelled mycotoxins as internal standards. The tested contamination ranges were from 27.7 to 378 μg/kg for NIV, from 234 to 2420 μg/kg for DON, from 18.5 to 137 μg/kg for 3-acetyl-DON, from 11.4 to 142 μg/kg for 15-acetyl-DON, from 2.1 to 37.6 μg/kg for T-2 toxin, from 6.6 to 134 μg/kg for HT-2 toxin, and from 31.6 to 230 μg/kg for ZEN. Recoveries were in the range 71–97% with the lowest values for NIV, the most polar mycotoxin. The relative standard deviation for repeatability (RSDr) was in the range of 2.2–34%, while the relative standard deviation for reproducibility (RSDR) was between 6.4% and 45%. The HorRat values ranged from 0.4 to 2.0. The results of the collaborative study showed that the candidate method is fit for the purpose of enforcing the legislative limits of the major Fusarium toxins in wheat and wheat-based products.

ACS Style

Annalisa De Girolamo; Biancamaria Ciasca; Michelangelo Pascale; Veronica M.T. Lattanzio. Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study. Toxins 2020, 12, 786 .

AMA Style

Annalisa De Girolamo, Biancamaria Ciasca, Michelangelo Pascale, Veronica M.T. Lattanzio. Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study. Toxins. 2020; 12 (12):786.

Chicago/Turabian Style

Annalisa De Girolamo; Biancamaria Ciasca; Michelangelo Pascale; Veronica M.T. Lattanzio. 2020. "Determination of Zearalenone and Trichothecenes, Including Deoxynivalenol and Its Acetylated Derivatives, Nivalenol, T-2 and HT-2 Toxins, in Wheat and Wheat Products by LC-MS/MS: A Collaborative Study." Toxins 12, no. 12: 786.

Journal article
Published: 01 December 2020 in Toxins
Reads 0
Downloads 0

An assessment of the natural ochratoxin A (OTA) exposure of seven Martina Franca jennies was carried out by analyzing blood and milk samples collected close to and after delivery. A total of 41 and 34 blood samples were collected from jennies and foals, respectively, and analyzed by ELISA. A total of 33 milk samples were collected from jennies and analyzed by the HPLC/FLD method based on IAC clean-up. Furthermore, 53 feed samples were collected from January to September and analyzed by a reference method (AOAC Official Method No. 2000.03) for OTA content. Feed samples showed OTA levels up to 2.7 ng/g with an incidence of 32%, while the OTA incidence rate in jennies’ blood samples was 73%, with a median value of 97 ng/L and concentrations ranging from

ACS Style

Vincenzo Lippolis; Shafaq Asif; Michelangelo Pascale; Salvatore Cervellieri; Erminia Mancini; Angelo Peli; Ippolito De Amicis; Domenico Robbe; Fiorenza Minervini. Natural Occurrence of Ochratoxin A in Blood and Milk Samples from Jennies and Their Foals after Delivery. Toxins 2020, 12, 758 .

AMA Style

Vincenzo Lippolis, Shafaq Asif, Michelangelo Pascale, Salvatore Cervellieri, Erminia Mancini, Angelo Peli, Ippolito De Amicis, Domenico Robbe, Fiorenza Minervini. Natural Occurrence of Ochratoxin A in Blood and Milk Samples from Jennies and Their Foals after Delivery. Toxins. 2020; 12 (12):758.

Chicago/Turabian Style

Vincenzo Lippolis; Shafaq Asif; Michelangelo Pascale; Salvatore Cervellieri; Erminia Mancini; Angelo Peli; Ippolito De Amicis; Domenico Robbe; Fiorenza Minervini. 2020. "Natural Occurrence of Ochratoxin A in Blood and Milk Samples from Jennies and Their Foals after Delivery." Toxins 12, no. 12: 758.

Journal article
Published: 27 October 2020 in Foods
Reads 0
Downloads 0

Italy is the country with the largest durum wheat pasta production and consumption. The mandatory labelling for pasta indicating the country of origin of wheat has made consumers more aware about the consumed pasta products and is influencing their choice towards 100% Italian wheat pasta. This aspect highlights the need to promote the use of domestic wheat as well as to develop rapid methodologies for the authentication of pasta. A rapid, inexpensive, and easy-to-use method based on infrared spectroscopy was developed and validated for authenticating pasta made with 100% Italian durum wheat. The study was conducted on pasta marketed in Italy and made with durum wheat cultivated in Italy (n = 176 samples) and on pasta made with mixtures of wheat cultivated in Italy and/or abroad (n = 185 samples). Pasta samples were analyzed by Fourier transform-near infrared (FT-NIR) spectroscopy coupled with supervised classification models. The good performance results of the validation set (sensitivity of 95%, specificity and accuracy of 94%) obtained using principal component-linear discriminant analysis (PC-LDA) clearly demonstrated the high prediction capability of this method and its suitability for authenticating 100% Italian durum wheat pasta. This output is of great interest for both producers of Italian pasta pointing toward authentication purposes of their products and consumer associations aimed to preserve and promote the typicity of Italian products.

ACS Style

Annalisa De Girolamo; Salvatore Cervellieri; Erminia Mancini; Michelangelo Pascale; Antonio Francesco Logrieco; Vincenzo Lippolis. Rapid Authentication of 100% Italian Durum Wheat Pasta by FT-NIR Spectroscopy Combined with Chemometric Tools. Foods 2020, 9, 1551 .

AMA Style

Annalisa De Girolamo, Salvatore Cervellieri, Erminia Mancini, Michelangelo Pascale, Antonio Francesco Logrieco, Vincenzo Lippolis. Rapid Authentication of 100% Italian Durum Wheat Pasta by FT-NIR Spectroscopy Combined with Chemometric Tools. Foods. 2020; 9 (11):1551.

Chicago/Turabian Style

Annalisa De Girolamo; Salvatore Cervellieri; Erminia Mancini; Michelangelo Pascale; Antonio Francesco Logrieco; Vincenzo Lippolis. 2020. "Rapid Authentication of 100% Italian Durum Wheat Pasta by FT-NIR Spectroscopy Combined with Chemometric Tools." Foods 9, no. 11: 1551.

Review
Published: 29 September 2020 in Microorganisms
Reads 0
Downloads 0

Toxigenic fungi and mycotoxins are very common in food crops, with noticeable differences in their host specificity in terms of pathogenicity and toxin contamination. In addition, such crops may be infected with mixtures of mycotoxigenic fungi, resulting in multi-mycotoxin contamination. Climate represents the key factor in driving the fungal community structure and mycotoxin contamination levels pre- and post-harvest. Thus, there is significant interest in understanding the impact of interacting climate change-related abiotic factors (especially increased temperature, elevated CO2 and extremes in water availability) on the relative risks of mycotoxin contamination and impacts on food safety and security. We have thus examined the available information from the last decade on relative risks of mycotoxin contamination under future climate change scenarios and identified the gaps in knowledge. This has included the available scientific information on the ecology, genomics, distribution of toxigenic fungi and intervention strategies for mycotoxin control worldwide. In addition, some suggestions for prediction and prevention of mycotoxin risks are summarized together with future perspectives and research needs for a better understanding of the impacts of climate change scenarios.

ACS Style

Giancarlo Perrone; Massimo Ferrara; Angel Medina; Michelangelo Pascale; Naresh Magan. Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk. Microorganisms 2020, 8, 1496 .

AMA Style

Giancarlo Perrone, Massimo Ferrara, Angel Medina, Michelangelo Pascale, Naresh Magan. Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk. Microorganisms. 2020; 8 (10):1496.

Chicago/Turabian Style

Giancarlo Perrone; Massimo Ferrara; Angel Medina; Michelangelo Pascale; Naresh Magan. 2020. "Toxigenic Fungi and Mycotoxins in a Climate Change Scenario: Ecology, Genomics, Distribution, Prediction and Prevention of the Risk." Microorganisms 8, no. 10: 1496.

Journal article
Published: 17 May 2020 in Toxins
Reads 0
Downloads 0

Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mechanical size separation of coarse, small and broken kernels, (ii) removal of dust/fine particles through an aspiration channel, (iii) separation of kernels based on gravity and (iv) optical sorting of spatial and spectral kernel defects. Depending on the sampled fraction, dynamic or static sampling was performed according to the Commission Regulation No. 401/2006 along the entire cleaning process lines. Aflatoxin analyses of the water–slurry aggregate samples were performed according to the AOAC Official Method No. 2005.008 based on high-performance liquid chromatography and immunoaffinity column cleanup of the extracts. A significant reduction in aflatoxin content in the cleaned products, ranging from 65% to 84% with respect to the uncleaned products, was observed when continuous cleaning lines were used. Additionally, an overall aflatoxin reduction from 55% to 94% was obtained by combining results from separate cleaning steps. High levels of aflatoxins (up to 490 µg/kg) were found in the rejected fractions, with the highest levels in dust and in the rejected fractions from the aspirator and optical sorting. This study shows that a cleaning line combining both mechanical and optical sorting technologies provides an efficient solution for reducing aflatoxin contamination in maize.

ACS Style

Michelangelo Pascale; Antonio F. Logrieco; Matthias Graeber; Marina Hirschberger; Mareike Reichel; Vincenzo Lippolis; Annalisa De Girolamo; Veronica M.T. Lattanzio; Katarina Slettengren. Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions. Toxins 2020, 12, 331 .

AMA Style

Michelangelo Pascale, Antonio F. Logrieco, Matthias Graeber, Marina Hirschberger, Mareike Reichel, Vincenzo Lippolis, Annalisa De Girolamo, Veronica M.T. Lattanzio, Katarina Slettengren. Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions. Toxins. 2020; 12 (5):331.

Chicago/Turabian Style

Michelangelo Pascale; Antonio F. Logrieco; Matthias Graeber; Marina Hirschberger; Mareike Reichel; Vincenzo Lippolis; Annalisa De Girolamo; Veronica M.T. Lattanzio; Katarina Slettengren. 2020. "Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions." Toxins 12, no. 5: 331.

Journal article
Published: 22 April 2020 in Toxins
Reads 0
Downloads 0

Aflatoxin B1 (AFB1) is a secondary metabolite produced by some Aspergillus spp. fungi affecting many crops and feed materials. Aflatoxin M1 (AFM1), the 4-hydroxylated metabolite of AFB1, is the main AFB1-related compound present in milk, and it is categorized by the International Agency for Research on Cancer (IARC) as a “group 1 human carcinogen”. The aim of this work was to evaluate and compare the analytical performances of two commercial immunoassays widely applied for the detection of AFM1 in milk, namely strip test immunoassay and enzyme linked immunosorbent assay (ELISA). Assay validation included samples at AFM1 levels of 25, 50, 75 ng/kg and blank samples (AFM1 < 0.5 ng/kg). With respect to a screening target concentration (STC) of 50 ng/kg the two assays showed cut-off values of 37.7 ng/kg and 47.5 ng/kg for strip test and ELISA, respectively, a false suspect rate for blanks

ACS Style

Ivan Pecorelli; Natascia Guarducci; Cristoph Von Holst; Rita Bibi; Michelangelo Pascale; Biancamaria Ciasca; Antonio F. Logrieco; Veronica M. T. Lattanzio. Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk. Toxins 2020, 12, 270 .

AMA Style

Ivan Pecorelli, Natascia Guarducci, Cristoph Von Holst, Rita Bibi, Michelangelo Pascale, Biancamaria Ciasca, Antonio F. Logrieco, Veronica M. T. Lattanzio. Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk. Toxins. 2020; 12 (4):270.

Chicago/Turabian Style

Ivan Pecorelli; Natascia Guarducci; Cristoph Von Holst; Rita Bibi; Michelangelo Pascale; Biancamaria Ciasca; Antonio F. Logrieco; Veronica M. T. Lattanzio. 2020. "Critical Comparison of Analytical Performances of Two Immunoassay Methods for Rapid Detection of Aflatoxin M1 in Milk." Toxins 12, no. 4: 270.

Journal article
Published: 01 November 2019 in Journal of AOAC INTERNATIONAL
Reads 0
Downloads 0

The co-occurrence of regulated mycotoxins in foods and feeds, together with modified (“masked”) and emerging mycotoxins, has been increasingly reported worldwide in recent years. Therefore, sensitive, accurate, and validated methods for the simultaneous determination of these hazardous contaminants in different matrices are highly demanded to fulfil regulatory requirements and to carry out reliable surveillance programs. In these last years, LC-MS methodologies for multimycotoxin screening and/or quantification are being routinely used in control laboratories. However, to date, only one European Standard for multimycotoxin determination is based on LC-MS (EN 16877:2016). The need for standardized LC-MS methods for multimycotoxin determination has been highlighted by regulatory authorities and scientific advisory bodies, including the U.S. Food and Drug Administration and the European Commission. The European Committee for Standardization (CEN) has issued calls for tender for the development of standardized LC-MS methods for mycotoxins in food and animal feeding stuffs. As deliverables, some LC-MS based methods for multimycotoxin determination are currently under approval as European Standards. In addition, the European Commission has recently established specific criteria with which screening methods for mycotoxins, including LC-MS methods, have to comply for use for regulatory purposes. Validation procedures by single-laboratory and collaborative trials have been defined. This paper provides insights and advances on guidelines and tools for performance evaluation of LC-MS methods intended for quantitative determination and for semiquantitative screening of multimycotoxins. In particular, performance criteria set in the European Union and the United States are critically overviewed, and expectations, needs, and future challenges relevant to LC-MS methods for multimycotoxin determination are also discussed.

ACS Style

Michelangelo Pascale; Annalisa De Girolamo; Vincenzo Lippolis; Joerg Stroka; Hans G J Mol; Veronica M T Lattanzio. Performance Evaluation of LC-MS Methods for Multimycotoxin Determination. Journal of AOAC INTERNATIONAL 2019, 102, 1708 -1720.

AMA Style

Michelangelo Pascale, Annalisa De Girolamo, Vincenzo Lippolis, Joerg Stroka, Hans G J Mol, Veronica M T Lattanzio. Performance Evaluation of LC-MS Methods for Multimycotoxin Determination. Journal of AOAC INTERNATIONAL. 2019; 102 (6):1708-1720.

Chicago/Turabian Style

Michelangelo Pascale; Annalisa De Girolamo; Vincenzo Lippolis; Joerg Stroka; Hans G J Mol; Veronica M T Lattanzio. 2019. "Performance Evaluation of LC-MS Methods for Multimycotoxin Determination." Journal of AOAC INTERNATIONAL 102, no. 6: 1708-1720.

Journal article
Published: 02 October 2019 in Foods
Reads 0
Downloads 0

Fourier transform near infrared (FT-NIR) spectroscopy, in combination with principal component-linear discriminant analysis (PC-LDA), was used for tracing the geographical origin of durum wheat samples. The classification model PC-LDA was applied to discriminate durum wheat samples originating from Northern, Central, and Southern Italy (n = 181), and to differentiate Italian durum wheat samples from those cultivated in other countries across the world (n = 134). Developed models were validated on a separated set of wheat samples. Different pre-treatments of spectral data and different spectral regions were selected and compared in terms of overall discrimination (OD) rates obtained in validation. The LDA models were able to correctly discriminate durum Italian wheat samples according to their geographical origin (i.e., North, Central, and South) with OD rates of up of 96.7%. Better results were obtained when LDA models were applied to the discrimination of Italian durum wheat samples from those originating from other countries across the world, having OD rates of up to 100%. The excellent results obtained herein clearly indicate the potential of FT-NIR spectroscopy to be used for the discrimination of durum wheat samples according to their geographical origin.

ACS Style

Annalisa De Girolamo; Marina Cortese; Salvatore Cervellieri; Vincenzo Lippolis; Michelangelo Pascale; Antonio Francesco Logrieco; Michele Suman. Tracing the Geographical Origin of Durum Wheat by FT-NIR Spectroscopy. Foods 2019, 8, 450 .

AMA Style

Annalisa De Girolamo, Marina Cortese, Salvatore Cervellieri, Vincenzo Lippolis, Michelangelo Pascale, Antonio Francesco Logrieco, Michele Suman. Tracing the Geographical Origin of Durum Wheat by FT-NIR Spectroscopy. Foods. 2019; 8 (10):450.

Chicago/Turabian Style

Annalisa De Girolamo; Marina Cortese; Salvatore Cervellieri; Vincenzo Lippolis; Michelangelo Pascale; Antonio Francesco Logrieco; Michele Suman. 2019. "Tracing the Geographical Origin of Durum Wheat by FT-NIR Spectroscopy." Foods 8, no. 10: 450.

Journal article
Published: 01 July 2019 in Toxins
Reads 0
Downloads 0

T-2 and HT-2 toxins and their main modified forms (T-2 glucoside and HT-2 glucoside) may co-occur in cereals and cereal-based products. A fluorescence polarization immunoassay (FPIA) was developed for the simultaneous determination of T-2 toxin, HT-2 toxin and relevant glucosides, expressed as sum. The developed FPIA, using a HT-2-specific antibody, showed high sensitivity (IC50 = 2.0 ng/mL) and high cross-reactivity (100% for T-2 toxin and 80% for T-2 and HT-2 glucosides). The FPIA has been used to develop two rapid and easy-to-use methods using two different extraction protocols, based on the use of organic (methanol/water, 90:10, v/v) and non-organic (water) solvents, for the determination of these toxins in wheat. The two proposed methods showed analytical performances in terms of sensitivity (LOD 10 µg/kg) recovery (92-97%) and precision (relative standard deviations ≤13%), fulfilling the criteria for acceptability of an analytical method for the quantitative determination of T-2 and HT-2 toxins established by the European Union. Furthermore, the methods were then validated in accordance with the harmonized guidelines for the validation of screening methods included in the Regulation (EU) No. 519/2014. The satisfactory analytical performances, in terms of intermediate precision (≤25%), cut-off level (80 and 96 µg/kg for the two methods) and rate of false positives (<0.1%) confirmed the applicability of the proposed methods as screening method for assessing the content of these toxins in wheat at the EU indicative levels reported for T-2 and HT-2 toxins.

ACS Style

Vincenzo Lippolis; Anna C. R. Porricelli; Erminia Mancini; Biancamaria Ciasca; Veronica M. T. Lattanzio; Annalisa De Girolamo; Chris M. Maragos; Susan McCormick; Peiwu Li; Antonio F. Logrieco; Michelangelo Pascale. Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat. Toxins 2019, 11, 380 .

AMA Style

Vincenzo Lippolis, Anna C. R. Porricelli, Erminia Mancini, Biancamaria Ciasca, Veronica M. T. Lattanzio, Annalisa De Girolamo, Chris M. Maragos, Susan McCormick, Peiwu Li, Antonio F. Logrieco, Michelangelo Pascale. Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat. Toxins. 2019; 11 (7):380.

Chicago/Turabian Style

Vincenzo Lippolis; Anna C. R. Porricelli; Erminia Mancini; Biancamaria Ciasca; Veronica M. T. Lattanzio; Annalisa De Girolamo; Chris M. Maragos; Susan McCormick; Peiwu Li; Antonio F. Logrieco; Michelangelo Pascale. 2019. "Fluorescence Polarization Immunoassay for the Determination of T-2 and HT-2 Toxins and Their Glucosides in Wheat." Toxins 11, no. 7: 380.

Journal article
Published: 20 February 2019 in Toxins
Reads 0
Downloads 0

(AFB1) in maize and wheat using LFD and LC-HRMS, respectively. The results of analyses were used to calculate intermediate precision (RSDip, covering the inter-analyst variability in preparing the analytical samples and the precision under repeatability conditions) cut-off values and false suspect rates. RSDip ranged from 6.5% to 30% for DON, and from 16% to 33% for AFB1. The highest obtained variances were associated with the AFB1 analyses due to working with much lower mass fractions. The rate of false suspect results were lower than 0.1% for all tested methods. All methods showed a fit-for-purpose method performance profile, which allowed a clear distinction of samples containing the analytes at the screening target concentration (STC) from negative control samples. Moreover, the first time users obtained method performances similar to those obtained for validation studies previously performed on the screening methods included in the training course.

ACS Style

Veronica M. T. Lattanzio; Christoph Von Holst; Vincenzo Lippolis; Annalisa De Girolamo; Antonio F. Logrieco; Hans G. J. Mol; Michelangelo Pascale. Evaluation of Mycotoxin Screening Tests in a Verification Study Involving First Time Users. Toxins 2019, 11, 129 .

AMA Style

Veronica M. T. Lattanzio, Christoph Von Holst, Vincenzo Lippolis, Annalisa De Girolamo, Antonio F. Logrieco, Hans G. J. Mol, Michelangelo Pascale. Evaluation of Mycotoxin Screening Tests in a Verification Study Involving First Time Users. Toxins. 2019; 11 (2):129.

Chicago/Turabian Style

Veronica M. T. Lattanzio; Christoph Von Holst; Vincenzo Lippolis; Annalisa De Girolamo; Antonio F. Logrieco; Hans G. J. Mol; Michelangelo Pascale. 2019. "Evaluation of Mycotoxin Screening Tests in a Verification Study Involving First Time Users." Toxins 11, no. 2: 129.

Journal article
Published: 10 January 2019 in Food Chemistry
Reads 0
Downloads 0

The use of infrared spectroscopic for the screening of 229 unprocessed durum wheat samples naturally contaminated with OTA has been investigated. Samples were analysed by both Fourier Transform near- and mid-infrared spectroscopy (FT-NIR, FT-MIR). Partial-Least Squares-Discriminant Analysis (PLS-DA) and Principal Component-Linear Discriminant Analysis (PC-LDA) classification models were used to differentiate highly contaminated durum wheat samples from low contaminated ones and the performances of the resulting models were compared. The overall discrimination rates were higher than 94% for both FT-NIR and FT-MIR range by using a cut-off limit set at 2 µg/kg OTA, independently from the classification model used thus confirming the reliability of the two statistical approaches used. False compliant rates of 6% were obtained for both spectral ranges and both classification models. These findings indicate that FT-NIR, as well as FT-MIR analysis, might be a promising, inexpensive and easy-to-use screening tool to rapidly discriminate unprocessed wheat samples for OTA content.

ACS Style

Annalisa De Girolamo; Christoph von Holst; Marina Cortese; Salvatore Cervellieri; Michelangelo Pascale; Francesco Longobardi; Lucia Catucci; Anna Chiara Raffaella Porricelli; Vincenzo Lippolis. Rapid screening of ochratoxin A in wheat by infrared spectroscopy. Food Chemistry 2019, 282, 95 -100.

AMA Style

Annalisa De Girolamo, Christoph von Holst, Marina Cortese, Salvatore Cervellieri, Michelangelo Pascale, Francesco Longobardi, Lucia Catucci, Anna Chiara Raffaella Porricelli, Vincenzo Lippolis. Rapid screening of ochratoxin A in wheat by infrared spectroscopy. Food Chemistry. 2019; 282 ():95-100.

Chicago/Turabian Style

Annalisa De Girolamo; Christoph von Holst; Marina Cortese; Salvatore Cervellieri; Michelangelo Pascale; Francesco Longobardi; Lucia Catucci; Anna Chiara Raffaella Porricelli; Vincenzo Lippolis. 2019. "Rapid screening of ochratoxin A in wheat by infrared spectroscopy." Food Chemistry 282, no. : 95-100.

Evaluation study
Published: 14 November 2018 in Journal of the Science of Food and Agriculture
Reads 0
Downloads 0

BACKGROUND Deoxynivalenol (DON) is the most common Fusarium mycotoxin occurring in wheat and derived products with several adverse and toxic effects in animals and humans. Although bran fractions produced by milling of wheat have numerous health benefits, cereal bran is the part of the grain with the highest concentration of DON thus representing a risk for consumers. Increased efforts have been made to develop analytical methods suitable for rapid DON screening. RESULTS The applicability of using Fourier transform near‐infrared (FT‐NIR), mid‐infrared (FT‐MIR) spectroscopy and their combination for rapid analysis of DON in wheat bran has been investigated for the classification of samples into compliant and non‐compliant regarding the EC legal limit of 750 μg kg‐1. Partial Least Squares‐Discriminant Analysis (PLS‐DA) and Principal Component‐Linear Discriminant Analysis (PC‐LDA) were employed as classification techniques using a cut‐off value of 400 μg kg‐1 DON to distinguish the two classes. Depending on the classification model, overall discrimination rates were from 87% to 91% for FT‐NIR and from 86% to 87% for FT‐MIR spectral range. The FT‐NIR spectroscopy gave the highest overall classification rate of wheat bran samples with no false compliant samples and 18% of false not compliant samples, when the PC‐LDA classification model was applied. The combination of the two spectral ranges did not provide a substantial improvement of classification results with respect to FT‐NIR. CONCLUSIONS FT‐NIR in combination with classification models was an efficient tool to screen many DON‐contaminated wheat bran samples and assess their compliance with EC regulation. This article is protected by copyright. All rights reserved.

ACS Style

Annalisa De Girolamo; Salvatore Cervellieri; Marina Cortese; Anna Chiara Raffaella Porricelli; Michelangelo Pascale; Francesco Longobardi; Christoph Von Holst; Leonardo Ciaccheri; Vincenzo Lippolis. Fourier transform near-infrared and mid-infrared spectroscopy as efficient tools for rapid screening of deoxynivalenol contamination in wheat bran. Journal of the Science of Food and Agriculture 2018, 99, 1946 -1953.

AMA Style

Annalisa De Girolamo, Salvatore Cervellieri, Marina Cortese, Anna Chiara Raffaella Porricelli, Michelangelo Pascale, Francesco Longobardi, Christoph Von Holst, Leonardo Ciaccheri, Vincenzo Lippolis. Fourier transform near-infrared and mid-infrared spectroscopy as efficient tools for rapid screening of deoxynivalenol contamination in wheat bran. Journal of the Science of Food and Agriculture. 2018; 99 (4):1946-1953.

Chicago/Turabian Style

Annalisa De Girolamo; Salvatore Cervellieri; Marina Cortese; Anna Chiara Raffaella Porricelli; Michelangelo Pascale; Francesco Longobardi; Christoph Von Holst; Leonardo Ciaccheri; Vincenzo Lippolis. 2018. "Fourier transform near-infrared and mid-infrared spectroscopy as efficient tools for rapid screening of deoxynivalenol contamination in wheat bran." Journal of the Science of Food and Agriculture 99, no. 4: 1946-1953.

Book chapter
Published: 26 October 2018 in Aptamers for Analytical Applications
Reads 0
Downloads 0

This chapter describes different aptamer immobilization chemistries used in the production of aptasensors, providing key examples of the most applied technologies. Aptamer‐based biosensors, assays, and diagnostics hold great potential for applications in medical, food safety, and environmental analyses. Typically, the requirements of the desired analytical methods for aptasensor development determine the choice of substrate and chemistry for aptamer immobilization. The chapter categorizes the immobilization based on substrate type: planar or flat gold; solid phase; and nanomaterials. It reviews some recent diagnostic applications in relation to the immobilization method. Several immobilization techniques have been developed in the past years. These include physical adsorption, covalent binding, self‐assembly, avidin‐biotin immobilization, and hybridization. Strategies for immobilization must be reliable, simple, and must allow the aptamers to retain their biophysical characteristics and binding abilities. The chapter provides an overview of the standard approaches for aptamer immobilization on an array of materials including the relevant advantages and limitations.

ACS Style

Annalisa De Girolamo; Maureen McKeague; Michelangelo Pascale; Marina Cortese; Maria C. DeRosa. Immobilization of Aptamers on Substrates. Aptamers for Analytical Applications 2018, 85 -126.

AMA Style

Annalisa De Girolamo, Maureen McKeague, Michelangelo Pascale, Marina Cortese, Maria C. DeRosa. Immobilization of Aptamers on Substrates. Aptamers for Analytical Applications. 2018; ():85-126.

Chicago/Turabian Style

Annalisa De Girolamo; Maureen McKeague; Michelangelo Pascale; Marina Cortese; Maria C. DeRosa. 2018. "Immobilization of Aptamers on Substrates." Aptamers for Analytical Applications , no. : 85-126.

Special issue article
Published: 10 July 2018 in Journal of Mass Spectrometry
Reads 0
Downloads 0

A strong trend toward using highly selective mass spectrometry technologies for screening of multiple mycotoxins has been observed in recent years. In the present study, the process of validation of a multi‐mycotoxin screening method based on liquid chromatography high‐resolution mass spectrometry method is presented. The method was intended for the simultaneous screening of the major Fusarium toxins (deoxynivalenol, 3‐ and 15‐acetyl deoxynivalenol, T‐2 and HT‐2 toxins, zearalenone, enniatins A, A1, B, B1, beauvericin) in wheat. The sample preparation protocol was based on a double extraction (methanol followed by acetonitrile/water mixture), and purification through solid phase extraction C18 column. To provide insights for full exploitation of the potential of the double stage HRMS detection a full scan acquisition event followed by a sequence of five fragmentation events (vDIA) were set for mycotoxin detection, the latter to be exploited for confirmatory purposes. Method analytical performances were evaluated through in house validation and small scale interlaboratory study, designed according to Commission Regulation 519/2014/EU, setting performance requirements for screening methods for mycotoxins. Screening target concentrations (STC) were close to EU maximum permitted or indicative levels. The in house validation provided the precision of the response under repeatability conditions and the intermediate precision (both resulting lower than 30%), the cut off value, and the rate of false suspect results for negative (free of the mycotoxin of interest) samples, that resulted lower than 0.1% in all cases. The collaborative study provided reproducibility and laboratory independent cut off values. Analysis of reference materials proved method trueness and suitability for screening of the major Fusarium mycotoxins in wheat. Finally the applicability of the full scan/vDIA detection approach was successfully tested on a set of naturally contaminated wheat samples, where two characteristic product ions could be detected for all identified mycotoxins even at levels in the low μg/kg range.

ACS Style

Biancamaria Ciasca; Michelangelo Pascale; Valerio Guido Altieri; Francesco Longobardi; Michele Suman; Dante Catellani; Veronica M.T. Lattanzio. In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat. Journal of Mass Spectrometry 2018, 53, 743 -752.

AMA Style

Biancamaria Ciasca, Michelangelo Pascale, Valerio Guido Altieri, Francesco Longobardi, Michele Suman, Dante Catellani, Veronica M.T. Lattanzio. In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat. Journal of Mass Spectrometry. 2018; 53 (9):743-752.

Chicago/Turabian Style

Biancamaria Ciasca; Michelangelo Pascale; Valerio Guido Altieri; Francesco Longobardi; Michele Suman; Dante Catellani; Veronica M.T. Lattanzio. 2018. "In-house validation and small-scale collaborative study to evaluate analytical performances of multimycotoxin screening methods based on liquid chromatography-high-resolution mass spectrometry: Case study on Fusarium toxins in wheat." Journal of Mass Spectrometry 53, no. 9: 743-752.

Evaluation study
Published: 13 May 2018 in Journal of the Science of Food and Agriculture
Reads 0
Downloads 0

BACKGROUND Deoxynivalenol (DON) is the mycotoxin, mainly produced by Fusarium species, most frequently occurring in cereals and cereal‐based products. Wheat bran is the outer layers of the kernel with a high risk of chemical hazards, including mycotoxins. Rapid methods for DON detection in wheat bran are highly demanded. RESULTS A rapid screening method using an electronic nose (e‐nose) based on metal oxide semiconductor sensors has been developed to distinguish wheat bran samples with different levels of DON contamination. A total of 470 naturally contaminated wheat bran samples was analysed by e‐nose analysis. Wheat bran samples were divided in two contamination classes: class A ([DON] ≤ 400 μg kg‐1, 225 samples) and class B ([DON] > 400 μg kg‐1, 245 samples). Discriminant Function Analysis (DFA), classified wheat bran samples with good mean recognition abilities in both calibration (92%) and validation (89%). In addition, a pattern of seventeen volatile compounds of wheat bran samples that were associated (positively or negatively) with DON content was characterized by HS‐SPME/GC‐MS. CONCLUSIONS These results indicate that the developed e‐nose method could be a useful tool for high throughput screening of DON‐contaminated wheat bran samples for their classification as acceptable/rejectable at contamination levels close to the EU maximum limit for DON, then reducing the number of samples to be analysed with a confirmatory method.

ACS Style

Vincenzo Lippolis; Salvatore Cervellieri; Anna Damascelli; Michelangelo Pascale; Annalisa Di Gioia; Francesco Longobardi; Annalisa De Girolamo. Rapid prediction of deoxynivalenol contamination in wheat bran by MOS-based electronic nose and characterization of the relevant pattern of volatile compounds. Journal of the Science of Food and Agriculture 2018, 98, 4955 -4962.

AMA Style

Vincenzo Lippolis, Salvatore Cervellieri, Anna Damascelli, Michelangelo Pascale, Annalisa Di Gioia, Francesco Longobardi, Annalisa De Girolamo. Rapid prediction of deoxynivalenol contamination in wheat bran by MOS-based electronic nose and characterization of the relevant pattern of volatile compounds. Journal of the Science of Food and Agriculture. 2018; 98 (13):4955-4962.

Chicago/Turabian Style

Vincenzo Lippolis; Salvatore Cervellieri; Anna Damascelli; Michelangelo Pascale; Annalisa Di Gioia; Francesco Longobardi; Annalisa De Girolamo. 2018. "Rapid prediction of deoxynivalenol contamination in wheat bran by MOS-based electronic nose and characterization of the relevant pattern of volatile compounds." Journal of the Science of Food and Agriculture 98, no. 13: 4955-4962.

Journals
Published: 27 November 2017 in Analytical Methods
Reads 0
Downloads 0

The analytical performances of a lateral flow immunoassay (AFLA-V AQUA™, Vicam a Waters Business) for the determination of aflatoxins in maize were evaluated according to Commission Regulation (EU) No. 519/2014.

ACS Style

Veronica M. T. Lattanzio; Natascia Guarducci; Stephen Powers; Biancamaria Ciasca; Michelangelo Pascale; Christoph von Holst. Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction. Analytical Methods 2017, 10, 123 -130.

AMA Style

Veronica M. T. Lattanzio, Natascia Guarducci, Stephen Powers, Biancamaria Ciasca, Michelangelo Pascale, Christoph von Holst. Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction. Analytical Methods. 2017; 10 (1):123-130.

Chicago/Turabian Style

Veronica M. T. Lattanzio; Natascia Guarducci; Stephen Powers; Biancamaria Ciasca; Michelangelo Pascale; Christoph von Holst. 2017. "Validation of a lateral flow immunoassay for the rapid determination of aflatoxins in maize by solvent free extraction." Analytical Methods 10, no. 1: 123-130.

Journal article
Published: 26 September 2017 in Toxins
Reads 0
Downloads 0

A rapid fluorescence polarization immunoassay (FPIA) was optimized and validated for the determination of ochratoxin A (OTA) in rye and rye crispbread. Samples were extracted with a mixture of acetonitrile/water (60:40, v/v) and purified by SPE-aminopropyl column clean-up before performing the FPIA. Overall mean recoveries were 86 and 95% for spiked rye and rye crispbread with relative standard deviations lower than 6%. Limits of detection (LOD) of the optimized FPIA was 0.6 μg/kg for rye and rye crispbread, respectively. Good correlations (r > 0.977) were observed between OTA contents in contaminated samples obtained by FPIA and high-performance liquid chromatography (HPLC) with immunoaffinity cleanup used as reference method. Furthermore, single laboratory validation and small-scale collaborative trials were carried out for the determination of OTA in rye according to Regulation 519/2014/EU laying down procedures for the validation of screening methods. The precision profile of the method, cut-off level and rate of false suspect results confirm the satisfactory analytical performances of assay as a screening method. These findings show that the optimized FPIA is suitable for high-throughput screening, and permits reliable quantitative determination of OTA in rye and rye crispbread at levels that fall below the EU regulatory limits.

ACS Style

Vincenzo Lippolis; Anna C. R. Porricelli; Marina Cortese; Michele Suman; Sandro Zanardi; Michelangelo Pascale. Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay. Toxins 2017, 9, 305 .

AMA Style

Vincenzo Lippolis, Anna C. R. Porricelli, Marina Cortese, Michele Suman, Sandro Zanardi, Michelangelo Pascale. Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay. Toxins. 2017; 9 (10):305.

Chicago/Turabian Style

Vincenzo Lippolis; Anna C. R. Porricelli; Marina Cortese; Michele Suman; Sandro Zanardi; Michelangelo Pascale. 2017. "Determination of Ochratoxin A in Rye and Rye-Based Products by Fluorescence Polarization Immunoassay." Toxins 9, no. 10: 305.

Book chapter
Published: 29 January 2017 in Advanced Structural Safety Studies
Reads 0
Downloads 0

This protocol specifies an accurate and sensitive method for the determination of T-2 and HT-2 toxins content in oats and oat-based foods using liquid chromatography with tandem mass spectrometry detection. T-2 and HT-2 toxins are extracted from the test material with a mixture of acetonitrile and water. The filtered extract is dried, reconstituted with a mixture of methanol and water, then purified on a polymeric solid-phase extraction cartridge. Toxins are finally eluted from the column with methanol and quantified by reversed phase high performance liquid chromatography with tandem mass spectrometry detection.

ACS Style

Veronica M. T. Lattanzio; Michelangelo Pascale. Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry. Advanced Structural Safety Studies 2017, 1536, 127 -136.

AMA Style

Veronica M. T. Lattanzio, Michelangelo Pascale. Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry. Advanced Structural Safety Studies. 2017; 1536 ():127-136.

Chicago/Turabian Style

Veronica M. T. Lattanzio; Michelangelo Pascale. 2017. "Determination of T-2 and HT-2 Toxins in Oats and Oat-Based Breakfast Cereals by Liquid-Chromatography Tandem Mass Spectrometry." Advanced Structural Safety Studies 1536, no. : 127-136.

Journal article
Published: 15 November 2016 in Toxins
Reads 0
Downloads 0

Ochratoxin A (OTA) is a mycotoxin produced as a secondary metabolite by several species of Aspergillus and Penicillium and frequently found as a natural contaminant in a wide range of food commodities. Novel and robust biorecognition agents for detecting this molecule are required. Aptamers are artificial nucleic acid ligands able to bind with high affinity and specificity to a given target molecule. In the last few years, three separate research groups have selected aptamers for ochratoxin A. While each of these three families of aptamers have been incorporated into various methods for detecting OTA, it is unclear if each aptamer candidate is better suited for a particular application. Here, we perform the first head-to-head comparison of solution-based binding parameters for these groups of aptamers. Based on our results, we provide recommendations for the appropriate choice of aptamer for incorporation into solution-based biorecognition assays and applications.

ACS Style

Maureen McKeague; Ranganathan Velu; Annalisa De Girolamo; Stefania Valenzano; Michelangelo Pascale; McKenzie Smith; Maria C. DeRosa. Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A. Toxins 2016, 8, 336 .

AMA Style

Maureen McKeague, Ranganathan Velu, Annalisa De Girolamo, Stefania Valenzano, Michelangelo Pascale, McKenzie Smith, Maria C. DeRosa. Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A. Toxins. 2016; 8 (11):336.

Chicago/Turabian Style

Maureen McKeague; Ranganathan Velu; Annalisa De Girolamo; Stefania Valenzano; Michelangelo Pascale; McKenzie Smith; Maria C. DeRosa. 2016. "Comparison of In-Solution Biorecognition Properties of Aptamers against Ochratoxin A." Toxins 8, no. 11: 336.