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Dr. Edna Ribeiro
Instituto Politécnico de Lisboa, Portugal

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0 biomonitoring
0 epigenetic
0 Endocrine disrupting compounds
0 Antibiotic alternatives
0 Microbiological diagnosis

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Preprint content
Published: 11 June 2021
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Background: Antimicrobial resistance of human pathogens such as methicillin-resistant Staphylococcus aureus (MRSA), is described by the World Health Organization as a health global challenge and efforts must be made for the discovery of new effective and safe compounds. This work aims to evaluate Epigallocatechin-3-gallate (EGCG) epigenetic and drug modulatory potential against S. aureus in vitro and in vivo. Methods: S. aureus strains were isolated from commensal flora of healthy volunteers. Antibiotic susceptibility and synergistic assay were assessed trough disk diffusion accordingly to EUCAST guidelines with and without co-exposure to EGCG at final concentrations of 250, 100, 50 and 25 µg/ml. Transcriptional expression of orfx, spdC and WalKR was performed trough qRT-PCR. A 90 days interventional study was performed with a daily consumption of 225 mg of EGCG. Results: Obtained data revealed a high prevalence of S. aureus colonization in health care workers and clearly demonstrate the antimicrobial and synergistic potential of EGCG as well as divergent resistant phenotypes associated with altered transcriptional expression of epigenetic and drug response modulators genes. Conclusions: Here we demonstrate the potential of EGCG for antimicrobial treatment and/or therapeutic adjuvant against antibiotic resistant microorganisms and report divergent patterns of epigenetic modulators expression associated with phenotypic resistance profiles.

ACS Style

Ana Sofia Zeferino; Ana Rita Mira; Mariana Delgadinho; Miguel Brito; Tomás Ponte; Edna Ribeiro. Drug Resistance and Epigenetic Modulatory Potential of Epigallocatechin-3-Gallate Against Staphylococcus Aureus. 2021, 1 .

AMA Style

Ana Sofia Zeferino, Ana Rita Mira, Mariana Delgadinho, Miguel Brito, Tomás Ponte, Edna Ribeiro. Drug Resistance and Epigenetic Modulatory Potential of Epigallocatechin-3-Gallate Against Staphylococcus Aureus. . 2021; ():1.

Chicago/Turabian Style

Ana Sofia Zeferino; Ana Rita Mira; Mariana Delgadinho; Miguel Brito; Tomás Ponte; Edna Ribeiro. 2021. "Drug Resistance and Epigenetic Modulatory Potential of Epigallocatechin-3-Gallate Against Staphylococcus Aureus." , no. : 1.

Journal article
Published: 31 March 2021 in Atmosphere
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This study aims to assess the airborne bioburden of rural and urban Portuguese Primary Health Care Centers (PHCC) using active and passive sampling methods and identify the potential differences in airborne microbiota between both environments. The highest total aerobic mesophilic bacterial load in indoor air were found in the Vaccination Room (448 CFU.m−3) in the Rural PHCC and in the Waiting Room (420 CFU.m−3) for Urban PHCC. The total coliforms contamination level in indoor air was detected only in the Cleaning Supplies Room (4 CFU.m−3) in the Urban PHCC. The most frequent bacteria genera identified was Micrococcus (21% Rural PHCC; 31% Urban PHCC). The surface samples showed a highest total aerobic mesophilic bacterial contamination in the Treatment Room (86 × 103 CFU.m−2) from the Rural PHCC and in the Front Office (200 × 103 CFU.m−2) from the Urban PHCC. The electrostatic dust cloth (EDC) samples showed a highest bacterial load in the Urban PHCC. Total aerobic mesophilic bacterial load in settled dust and in the Heating, Ventilating and Air Conditioning (HVAC) filter samples in the Urban PHCC (8 CFU.g−1 and 6 × 103 CFU.m−2) presented higher values compared with the Rural PHCC (1 CFU.g−1 and 2.5 × 103 CFU.m−2). Urban PHCC presented higher bacterial airborne contamination compared with the Rural PHCC for the majority of sampling sites, and when compared with the indoor air quality (IAQ) Portuguese legislation it was the Rural PHCC in two sampling places who did not comply with the established criteria.

ACS Style

Ana Monteiro; Beatriz Almeida; Inês Paciência; João Cavaleiro Rufo; Edna Ribeiro; Elisabete Carolino; Carla Viegas; António Uva; Sandra Verde. Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings. Atmosphere 2021, 12, 450 .

AMA Style

Ana Monteiro, Beatriz Almeida, Inês Paciência, João Cavaleiro Rufo, Edna Ribeiro, Elisabete Carolino, Carla Viegas, António Uva, Sandra Verde. Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings. Atmosphere. 2021; 12 (4):450.

Chicago/Turabian Style

Ana Monteiro; Beatriz Almeida; Inês Paciência; João Cavaleiro Rufo; Edna Ribeiro; Elisabete Carolino; Carla Viegas; António Uva; Sandra Verde. 2021. "Bacterial Contamination in Health Care Centers: Differences between Urban and Rural Settings." Atmosphere 12, no. 4: 450.

Journal article
Published: 09 April 2020 in High-Throughput
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Epigallocatechin-3-gallate (EGCG), the major catechin present in green tea, presents diverse appealing biological activities, such as antioxidative, anti-inflammatory, antimicrobial, and antiviral activities, among others. The present work evaluated the impact in the molecular profile of human plasma from daily consumption of 225 mg of EGCG for 90 days. Plasma from peripheral blood was collected from 30 healthy human volunteers and analyzed by high-throughput Fourier transform infrared spectroscopy. To capture the biochemical information while minimizing the interference of physical phenomena, several combinations of spectra pre-processing methods were evaluated by principal component analysis. The pre-processing method that led to the best class separation, that is, between the plasma spectral data collected at the beginning and after the 90 days, was a combination of atmospheric correction with a second derivative spectra. A hierarchical cluster analysis of second derivative spectra also highlighted the fact that plasma acquired before EGCG consumption presented a distinct molecular profile after the 90 days of EGCG consumption. It was also possible by partial least squares regression discriminant analysis to correctly predict all unlabeled plasma samples (not used for model construction) at both timeframes. We observed that the similarity in composition among the plasma samples was higher in samples collected after EGCG consumption when compared with the samples taken prior to EGCG consumption. Diverse negative peaks of the normalized second derivative spectra, associated with lipid and protein regions, were significantly affected (p < 0.001) by EGCG consumption, according to the impact of EGCG consumption on the patients’ blood, low density and high density lipoproteins ratio. In conclusion, a single bolus dose of 225 mg of EGCG, ingested throughout a period of 90 days, drastically affected plasma molecular composition in all participants, which raises awareness regarding prolonged human exposure to EGCG. Because the analysis was conducted in a high-throughput, label-free, and economic analysis, it could be applied to high-dimension molecular epidemiological studies to further promote the understanding of the effect of bio-compound consumption mode and frequency.

ACS Style

Rúben Araújo; Luís Ramalhete; Helder Da Paz; Edna Ribeiro; Cecília R.C. Calado. A Simple, Label-Free, and High-Throughput Method to Evaluate the Epigallocatechin-3-Gallate Impact in Plasma Molecular Profile. High-Throughput 2020, 9, 9 .

AMA Style

Rúben Araújo, Luís Ramalhete, Helder Da Paz, Edna Ribeiro, Cecília R.C. Calado. A Simple, Label-Free, and High-Throughput Method to Evaluate the Epigallocatechin-3-Gallate Impact in Plasma Molecular Profile. High-Throughput. 2020; 9 (2):9.

Chicago/Turabian Style

Rúben Araújo; Luís Ramalhete; Helder Da Paz; Edna Ribeiro; Cecília R.C. Calado. 2020. "A Simple, Label-Free, and High-Throughput Method to Evaluate the Epigallocatechin-3-Gallate Impact in Plasma Molecular Profile." High-Throughput 9, no. 2: 9.

Journal article
Published: 29 August 2019 in Toxics
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The worldwide production of synthetic chemicals, including endocrine disruptor chemicals (EDCs), such as Bisphenol A (BPA) has increased significantly in the last two decades. Human exposure to BPA, particularly through ingestion, is continuous and ubiquitous. Although, considered a weak environmental estrogen, BPA can induce divergent biological responses through several signaling pathways, including carcinogenesis in hormone-responsive organs. However, and despite the continuous increase of tumor cell-resistance to therapeutic drugs, such as doxorubicin (DOX), information regarding BPA drug interactions is still scarce, although its potential role in chemo-resistance has been suggested. This study aims to assess the potential interactions between environmentally relevant levels of BPA and DOX at a therapeutic dosage on Hep-2 and MRC-5 cell lines transciptome. Transcriptional effects in key-player genes for cancer biology, namely c-fos, p21, and bcl-xl, were evaluated through qRT-PCR. The cellular response was analyzed after exposure to BPA, DOX, or co-exposure to both chemicals. Transcriptional analysis showed that BPA exposure induces upregulation of bcl-xl and endorses an antagonistic non-monotonic response on DOX transcriptional effects. Moreover, the BPA interaction with DOX on c-fos and p21 expression emphasize its cellular specificity and divergent effects. Overall, Hep-2 was more susceptible to BPA effects in a dose-dependent manner while MRC-5 transcriptional levels endorsed a non-monotonic response. Our data indicate that BPA environmental exposure may influence chemotherapy outcomes, which emphasize the urgency for a better understanding of BPA interactions with chemotherapeutic agents, in the context of risk assessment.

ACS Style

Edna Ribeiro; Mariana Delgadinho; Miguel Brito. Environmentally Relevant Concentrations of Bisphenol A Interact with Doxorubicin Transcriptional Effects in Human Cell Lines. Toxics 2019, 7, 43 .

AMA Style

Edna Ribeiro, Mariana Delgadinho, Miguel Brito. Environmentally Relevant Concentrations of Bisphenol A Interact with Doxorubicin Transcriptional Effects in Human Cell Lines. Toxics. 2019; 7 (3):43.

Chicago/Turabian Style

Edna Ribeiro; Mariana Delgadinho; Miguel Brito. 2019. "Environmentally Relevant Concentrations of Bisphenol A Interact with Doxorubicin Transcriptional Effects in Human Cell Lines." Toxics 7, no. 3: 43.

Journal article
Published: 01 February 2019 in Toxins
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Swine production workers are exposed simultaneously to multiple contaminants. Occupational exposure to aflatoxin B1 (AFB1) in Portuguese swine production farms has already been reported. However, besides AFB1, data regarding fungal contamination showed that exposure to other mycotoxins could be expected in this setting. The present study aimed to characterize the occupational exposure to multiple mycotoxins of swine production workers. To provide a broad view on the burden of contamination by mycotoxins and the workers’ exposure, biological (urine) samples from workers (n = 25) and 38 environmental samples (air samples, n = 23; litter samples, n = 5; feed samples, n = 10) were collected. The mycotoxins biomarkers detected in the urine samples of the workers group were the deoxynivalenol-glucuronic acid conjugate (60%), aflatoxin M1 (16%), enniatin B (4%), citrinin (8%), dihydrocitrinone (12%) and ochratoxin A (80%). Results of the control group followed the same pattern, but in general with a lower number of quantifiable results (

ACS Style

Susana Viegas; Ricardo Assunção; Carla Martins; Carla Nunes; Bernd Osteresch; Magdalena Twarużek; Robert Kosicki; Jan Grajewski; Edna Ribeiro; Carla Viegas. Occupational Exposure to Mycotoxins in Swine Production: Environmental and Biological Monitoring Approaches. Toxins 2019, 11, 78 .

AMA Style

Susana Viegas, Ricardo Assunção, Carla Martins, Carla Nunes, Bernd Osteresch, Magdalena Twarużek, Robert Kosicki, Jan Grajewski, Edna Ribeiro, Carla Viegas. Occupational Exposure to Mycotoxins in Swine Production: Environmental and Biological Monitoring Approaches. Toxins. 2019; 11 (2):78.

Chicago/Turabian Style

Susana Viegas; Ricardo Assunção; Carla Martins; Carla Nunes; Bernd Osteresch; Magdalena Twarużek; Robert Kosicki; Jan Grajewski; Edna Ribeiro; Carla Viegas. 2019. "Occupational Exposure to Mycotoxins in Swine Production: Environmental and Biological Monitoring Approaches." Toxins 11, no. 2: 78.

Journal article
Published: 01 December 2018 in Archives of Industrial Hygiene and Toxicology
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Literature about occupational health in small-animal veterinary practices is scarce, but most of it has recognised a number of risks to be considered, including organic dust exposure. The aim of this pilot study was to assess organic dust, bacterial, and fungal contamination in the indoor environment of a typical Portuguese veterinary clinic but also to screen for azoleresistant fungi. To complement these findings we also analysed workers’ nasal exudates for resistant bacteriota. Particles measurements included mass concentrations (PMC) of five particle sizes (PM0.5, PM1, PM2.5, PM5, PM10) and their counts (PNC). Indoor air samples were obtained from six locations as well as before and during cat dental cleaning and cultured on four media for bacterial and fungal assessment. An outdoor sample was also collected for reference Surface samples were taken from the same indoor locations using swabs and we also use electrostatic dust cloths as passive methods. PM10 showed the highest concentrations across the locations. Indoor air fungal loads ranged from 88 to 504 CFU m−3. The azole-resistant Aspergillus section Nigri was identified in one sample. Indoor air bacterial loads ranged from 84 to 328 CFU m-3. Nasopharyngeal findings in the 14 veterinary clinic workers showed a remarkably low prevalence of Staphylococcus aureus (7.1 %). Our results point to contamination with organic dusts above the WHO limits and to the need for better ventilation. Future studies should combine the same sampling protocol (active and passive methods) with molecular tools to obtain more accurate risk characterisation. In terms of prevention, animals should be caged in rooms separate from where procedures take place, and worker protection should be observed at all times.

ACS Style

Carla Viegas; Ana Monteiro; Edna Ribeiro; Liliana Aranha Caetano; Elisabete Carolino; Ricardo Assunção; Susana Viegas. Organic dust exposure in veterinary clinics: a case study of a small-animal practice in Portugal. Archives of Industrial Hygiene and Toxicology 2018, 69, 309 -316.

AMA Style

Carla Viegas, Ana Monteiro, Edna Ribeiro, Liliana Aranha Caetano, Elisabete Carolino, Ricardo Assunção, Susana Viegas. Organic dust exposure in veterinary clinics: a case study of a small-animal practice in Portugal. Archives of Industrial Hygiene and Toxicology. 2018; 69 (4):309-316.

Chicago/Turabian Style

Carla Viegas; Ana Monteiro; Edna Ribeiro; Liliana Aranha Caetano; Elisabete Carolino; Ricardo Assunção; Susana Viegas. 2018. "Organic dust exposure in veterinary clinics: a case study of a small-animal practice in Portugal." Archives of Industrial Hygiene and Toxicology 69, no. 4: 309-316.

Journal article
Published: 27 November 2018 in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
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Bisphenol A (BPA) is one of the most widely utilized endocrine disruptors to which humans are exposed, particularity through ingestion. BPA is an aneugenic compound with a putative association to tumorigenesis. Although extensively studied in estrogen responsive cells, information regarding its effects on cells from the upper gastrointestinal tract exposed to free/active forms of BPA is still scarce. Similarly, BPA interactions with other drugs have been neglected, although it has been suggested to have a potential role in doxorubicin (DOX) chemoresistance. This study is intended to assess potential cytotoxic and genotoxic effects of BPA, as well as its interactions with DOX, in Human epithelial type 2 cells (Hep-2) originated from a human laryngeal carcinoma and in a DNA damage responsive cell line, the human lung fibroblasts (MRC-5). Cell viability was analyzed through the resazurin assay. The G protein-coupled estrogen receptor 1 (GPER) expression was visualized by immunodetection. Genotoxicity, namely DNA damage and oxidative DNA damage, were assessed by comet assay and micronuclei induction, and mitotic disruption was evaluated cytologically by fluorescent microscopy with DAPI staining. Cytotoxicity analysis showed that exposure to BPA per se does not affect cellular viability. Nevertheless, the genotoxic analysis showed that BPA induced an increase of DNA damage in the Hep-2 cell line and in oxidative damage in the MRC-5 cell line. An increase of micronuclei was also observed in both cell lines following BPA exposure. BPA and DOX co-exposures suggested that BPA acts as an antagonist of DOX effects in both cell lines. The interaction with DOX appears to be cell type dependent, exhibiting a non-monotonic response curve in MRC-5 cells, a GPER expressing cell line. Our study emphasizes the need for a deeper knowledge of BPA interactions, particularly with chemotherapeutic agents, in the context of risk assessment and public health.

ACS Style

Carina Ramos; Carina Ladeira; Sofia Zeferino; Ana Dias; Isabel Faria; Elisabete Cristovam; Manuel Gomes; Edna Ribeiro. Cytotoxic and genotoxic effects of environmental relevant concentrations of bisphenol A and interactions with doxorubicin. Mutation Research/Genetic Toxicology and Environmental Mutagenesis 2018, 838, 28 -36.

AMA Style

Carina Ramos, Carina Ladeira, Sofia Zeferino, Ana Dias, Isabel Faria, Elisabete Cristovam, Manuel Gomes, Edna Ribeiro. Cytotoxic and genotoxic effects of environmental relevant concentrations of bisphenol A and interactions with doxorubicin. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2018; 838 ():28-36.

Chicago/Turabian Style

Carina Ramos; Carina Ladeira; Sofia Zeferino; Ana Dias; Isabel Faria; Elisabete Cristovam; Manuel Gomes; Edna Ribeiro. 2018. "Cytotoxic and genotoxic effects of environmental relevant concentrations of bisphenol A and interactions with doxorubicin." Mutation Research/Genetic Toxicology and Environmental Mutagenesis 838, no. : 28-36.

Communication
Published: 11 June 2018 in International Journal of Environmental Research and Public Health
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Endocrine disruptors (EDs) belong to large and diverse groups of agents that may cause multiple biological effects associated with, for example, hormone imbalance and infertility, chronic diseases such as diabetes, genome damage and cancer. The health risks related with the exposure to EDs are typically underestimated, less well characterized, and not regulated to the same extent as, for example, carcinogens. The increased production and utilization of identified or suspected EDs in many different technological processes raises new challenges with respect to occupational exposure settings and associated health risks. Due to the specific profile of health risk, occupational exposure to EDs demands a new paradigm in health risk assessment, redefinition of exposure assessment, new effects biomarkers for occupational health surveillance and definition of limit values. The construction and plastics industries are among the strongest economic sectors, employing millions of workers globally. They also use large quantities of chemicals that are known or suspected EDs. Focusing on these two industries, this short communication discusses: (a) why occupational exposure to EDs needs a more specific approach to occupational health risk assessments, (b) identifies the current knowledge gaps, and (c) identifies and gives a rationale for a future occupational health paradigm, which will include ED biomarkers as a relevant parameter in occupational health risk assessment, surveillance and exposure prevention.

ACS Style

Aleksandra Fucic; Karen S. Galea; Radu Corneliu Duca; Mounia El Yamani; Nadine Frery; Lode Godderis; Thórhallur Ingi Halldórsson; Ivo Iavicoli; Sophie Ndaw; Edna Ribeiro; Susana Viegas; Hanns Moshammer. Potential Health Risk of Endocrine Disruptors in Construction Sector and Plastics Industry: A New Paradigm in Occupational Health. International Journal of Environmental Research and Public Health 2018, 15, 1229 .

AMA Style

Aleksandra Fucic, Karen S. Galea, Radu Corneliu Duca, Mounia El Yamani, Nadine Frery, Lode Godderis, Thórhallur Ingi Halldórsson, Ivo Iavicoli, Sophie Ndaw, Edna Ribeiro, Susana Viegas, Hanns Moshammer. Potential Health Risk of Endocrine Disruptors in Construction Sector and Plastics Industry: A New Paradigm in Occupational Health. International Journal of Environmental Research and Public Health. 2018; 15 (6):1229.

Chicago/Turabian Style

Aleksandra Fucic; Karen S. Galea; Radu Corneliu Duca; Mounia El Yamani; Nadine Frery; Lode Godderis; Thórhallur Ingi Halldórsson; Ivo Iavicoli; Sophie Ndaw; Edna Ribeiro; Susana Viegas; Hanns Moshammer. 2018. "Potential Health Risk of Endocrine Disruptors in Construction Sector and Plastics Industry: A New Paradigm in Occupational Health." International Journal of Environmental Research and Public Health 15, no. 6: 1229.

Website
Published: 14 March 2018 in Occupational Safety and Hygiene VI
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ACS Style

E. Ribeiro; A. Pereira; C. Vieira; I. Paulos; M. Marques; T. Swart; A. Monteiro. Bacteria bioburden assessment and MRSA colonization of workers and animals from a Portuguese swine production: A case report. Occupational Safety and Hygiene VI 2018, 351 -354.

AMA Style

E. Ribeiro, A. Pereira, C. Vieira, I. Paulos, M. Marques, T. Swart, A. Monteiro. Bacteria bioburden assessment and MRSA colonization of workers and animals from a Portuguese swine production: A case report. Occupational Safety and Hygiene VI. 2018; ():351-354.

Chicago/Turabian Style

E. Ribeiro; A. Pereira; C. Vieira; I. Paulos; M. Marques; T. Swart; A. Monteiro. 2018. "Bacteria bioburden assessment and MRSA colonization of workers and animals from a Portuguese swine production: A case report." Occupational Safety and Hygiene VI , no. : 351-354.

Book chapter
Published: 05 October 2017 in Exposure to Microbiological Agents in Indoor and Occupational Environments
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ACS Style

Edna Ribeiro; Carla Viegas; Susana Viegas; Anita Gomes; Martin Täubel; Raquel Sabino. Virus Bioburden in Hospital Environment. Exposure to Microbiological Agents in Indoor and Occupational Environments 2017, 329 -332.

AMA Style

Edna Ribeiro, Carla Viegas, Susana Viegas, Anita Gomes, Martin Täubel, Raquel Sabino. Virus Bioburden in Hospital Environment. Exposure to Microbiological Agents in Indoor and Occupational Environments. 2017; ():329-332.

Chicago/Turabian Style

Edna Ribeiro; Carla Viegas; Susana Viegas; Anita Gomes; Martin Täubel; Raquel Sabino. 2017. "Virus Bioburden in Hospital Environment." Exposure to Microbiological Agents in Indoor and Occupational Environments , no. : 329-332.

Book chapter
Published: 05 October 2017 in Exposure to Microbiological Agents in Indoor and Occupational Environments
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ACS Style

Edna Ribeiro; Isabel Faria; Carla Viegas; Susana Viegas; Anita Gomes; Martin Täubel; Raquel Sabino. Analyses Approaches for Bacteria. Exposure to Microbiological Agents in Indoor and Occupational Environments 2017, 97 -108.

AMA Style

Edna Ribeiro, Isabel Faria, Carla Viegas, Susana Viegas, Anita Gomes, Martin Täubel, Raquel Sabino. Analyses Approaches for Bacteria. Exposure to Microbiological Agents in Indoor and Occupational Environments. 2017; ():97-108.

Chicago/Turabian Style

Edna Ribeiro; Isabel Faria; Carla Viegas; Susana Viegas; Anita Gomes; Martin Täubel; Raquel Sabino. 2017. "Analyses Approaches for Bacteria." Exposure to Microbiological Agents in Indoor and Occupational Environments , no. : 97-108.

Book chapter
Published: 05 October 2017 in Exposure to Microbiological Agents in Indoor and Occupational Environments
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Through human history viruses have shown enormous epidemiological and pandemic potential as the occurrence and spread of viruses in pandemic dimensions poses a threat to the health and lives of seven billion people worldwide. Scientific evidence has associated harmful health effects to indoor air hazards recognizing the existence of a vital concern in public health sector. Thus the assessment of human exposure to biological aerosols and droplets indoor became an imperative requirement of investigation. Environmental bioburden assessment of viruses relies in both culture-dependent approaches that comprise classical methodologies, still prominent and vital in the field of modern biotechnology, and culture-independent approaches based on nucleic acid amplification techniques, which are considered the gold standard in clinical virology. The main factor influencing indoor microbiology is the human being and their activities. Indoor environments to be considered are those regularly occupied by humans: residences, offices, schools, industrial buildings, health care facilities, farming activities and other settings occupied all the time, or in which occupant density is high. It’s well known that approximately 60% of total human respiratory and gastrointestinal infections are acquired indoor, since viruses have a rapid spread in the community and can be transmitted easily, especially in crowded and poorly ventilated environments, causing high morbidity and decline in quality of life and productivity. Studies have shown that respiratory syncytial virus, rhinovirus, metapneumovirus, influenza and parainfluenza virus, and human enterovirus infections may be associated with virus-induced asthma, leading to diseases such as pneumonia. Gastroenteritis infectious (about 30±40% of cases) is attributable to viruses. Rotavirus, Astrovirus, Norwalk-like viruses and other caliciviruses are responsible for 48% of all reported outbreaks of infectious intestinal disease. Safe working conditions are essential for healthy living, that’s why the programmes conceived as a result of strategic and preventive policy maintenance, in refrigeration and ventilation systems, are the determining factor for the control of biological pollutants. Moreover, the development of highly sensitive and specific detection and identification methodologies with capacity to be used in diverse applications, such as diagnosis, public health risk assessment, research and for the implementation of preventive measures and protocols are imperative.

ACS Style

Edna Ribeiro; Céu Leitão; Elisabete Cristovam; Ana Dias. Viruses Present Indoors and Analyses Approaches. Exposure to Microbiological Agents in Indoor and Occupational Environments 2017, 129 -155.

AMA Style

Edna Ribeiro, Céu Leitão, Elisabete Cristovam, Ana Dias. Viruses Present Indoors and Analyses Approaches. Exposure to Microbiological Agents in Indoor and Occupational Environments. 2017; ():129-155.

Chicago/Turabian Style

Edna Ribeiro; Céu Leitão; Elisabete Cristovam; Ana Dias. 2017. "Viruses Present Indoors and Analyses Approaches." Exposure to Microbiological Agents in Indoor and Occupational Environments , no. : 129-155.

Review
Published: 13 September 2017 in Toxics
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Bisphenol A (BPA), 2,2-bis(4-hydroxyphenyl) propane, is one of the most utilized industrial chemicals worldwide, with the ability to interfere with/or mimic estrogenic hormones with associated biological responses. Environmental human exposure to this endocrine disruptor, mostly through oral intake, is considered a generalized phenomenon, particularly in developed countries. However, in the context of occupational exposure, non-dietary exposure sources (e.g., air and contact) cannot be underestimated. Here, we performed a review of the literature on BPA occupational exposure and associated health effects. Relevantly, the authors only identified 19 studies from 2009 to 2017 that demonstrate that occupationally exposed individuals have significantly higher detected BPA levels than environmentally exposed populations and that the detection rate of serum BPA increases in relation to the time of exposure. However, only 12 studies performed in China have correlated potential health effects with detected BPA levels, and shown that BPA-exposed male workers are at greater risk of male sexual dysfunction across all domains of sexual function; also, endocrine disruption, alterations to epigenetic marks (DNA methylation) and epidemiological evidence have shown significant effects on the offspring of parents exposed to BPA during pregnancy. This overview raises awareness of the dramatic and consistent increase in the production and exposure of BPA and creates urgency to assess the actual exposure of workers to this xenoestrogen and to evaluate potential associated adverse health effects.

ACS Style

Edna Ribeiro; Carina Ladeira; Susana Viegas. Occupational Exposure to Bisphenol A (BPA): A Reality That Still Needs to Be Unveiled. Toxics 2017, 5, 22 .

AMA Style

Edna Ribeiro, Carina Ladeira, Susana Viegas. Occupational Exposure to Bisphenol A (BPA): A Reality That Still Needs to Be Unveiled. Toxics. 2017; 5 (3):22.

Chicago/Turabian Style

Edna Ribeiro; Carina Ladeira; Susana Viegas. 2017. "Occupational Exposure to Bisphenol A (BPA): A Reality That Still Needs to Be Unveiled." Toxics 5, no. 3: 22.

Books book
Published: 20 March 2017 in Occupational Safety and Hygiene V
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ACS Style

C Viegas; V Santos; R Moreira; Tiago Faria; E Ribeiro; L Aranha; S Viegas. Worker’s nasal swab: A tool for occupational exposure assessment to bioburden? Occupational Safety and Hygiene V 2017, 277 -282.

AMA Style

C Viegas, V Santos, R Moreira, Tiago Faria, E Ribeiro, L Aranha, S Viegas. Worker’s nasal swab: A tool for occupational exposure assessment to bioburden? Occupational Safety and Hygiene V. 2017; ():277-282.

Chicago/Turabian Style

C Viegas; V Santos; R Moreira; Tiago Faria; E Ribeiro; L Aranha; S Viegas. 2017. "Worker’s nasal swab: A tool for occupational exposure assessment to bioburden?" Occupational Safety and Hygiene V , no. : 277-282.

Review
Published: 20 March 2017 in Occupational Safety and Hygiene V
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Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide. Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide. Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide. Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide. Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide. Occupational Safety and Hygiene V contains selected contributions from the International Symposium on Occupational Safety and Hygiene (SHO 2017, 10-11 April 2017, Guimarães, Portugal). The contributions focus on a wide range of topics, including: - occupational safety- risk assessment- safety management- ergonomics- management systems- environmental ergonomics- physical environments- construction safety, and- human factors Occupational Safety and Hygiene V is mainly based on research carried out at universities and other research institutions, but also includes practical studies developed by OHS Practitioners within companies. Accordingly, this book will be a helpful text to get acquainted with the state-of-the-art in research in these domains, as well as with some practical tools and approaches that are currently used by OHS professionals worldwide.

ACS Style

E Ribeiro; A Zeferino. Livestock-associated MRSA colonization of occupational exposed workers and households in Europe:A review. Occupational Safety and Hygiene V 2017, 1 .

AMA Style

E Ribeiro, A Zeferino. Livestock-associated MRSA colonization of occupational exposed workers and households in Europe:A review. Occupational Safety and Hygiene V. 2017; ():1.

Chicago/Turabian Style

E Ribeiro; A Zeferino. 2017. "Livestock-associated MRSA colonization of occupational exposed workers and households in Europe:A review." Occupational Safety and Hygiene V , no. : 1.

Review
Published: 07 February 2017 in Toxics
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Endocrine disrupting chemicals (EDCs) are exogenous chemicals that may occur naturally (e.g., phytoestrogens), while others are industrial substances and plasticizers commonly utilized worldwide to which human exposure, particularly at low-doses, is omnipresent, persistent and occurs in complex mixtures. EDCs can interfere with/or mimic estrogenic hormones and, consequently, can simultaneously trigger diverse signaling pathways which result in diverse and divergent biological responses. Additionally, EDCs can also bioaccumulate in lipid compartments of the organism forming a mixed “body burden” of contaminants. Although the independent action of chemicals has been considered the main principle in EDCs mixture toxicity, recent studies have demonstrated that numerous effects cannot be predicted when analyzing single compounds independently. Co-exposure to these agents, particularly in critical windows of exposure, may induce hazardous health effects potentially associated with a complex “body burden” of different origins. Here, we performed an exhaustive review of the available literature regarding EDCs mixtures exposure, toxicity mechanisms and effects, particularly at the most vulnerable human life stages. Although the assessment of potential risks to human health due to exposure to EDCs mixtures is a major topic for consumer safety, information regarding effective mixtures effects is still scarce.

ACS Style

Edna Ribeiro; Carina Ladeira; Susana Viegas. EDCs Mixtures: A Stealthy Hazard for Human Health? Toxics 2017, 5, 5 .

AMA Style

Edna Ribeiro, Carina Ladeira, Susana Viegas. EDCs Mixtures: A Stealthy Hazard for Human Health? Toxics. 2017; 5 (1):5.

Chicago/Turabian Style

Edna Ribeiro; Carina Ladeira; Susana Viegas. 2017. "EDCs Mixtures: A Stealthy Hazard for Human Health?" Toxics 5, no. 1: 5.

Conference abstract
Published: 04 September 2016 in Toxicology Letters
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ACS Style

E. Ribeiro; C. Ladeira; S. Viegas. EDCs mixture effects in human cell lines. Toxicology Letters 2016, 258, S320 .

AMA Style

E. Ribeiro, C. Ladeira, S. Viegas. EDCs mixture effects in human cell lines. Toxicology Letters. 2016; 258 ():S320.

Chicago/Turabian Style

E. Ribeiro; C. Ladeira; S. Viegas. 2016. "EDCs mixture effects in human cell lines." Toxicology Letters 258, no. : S320.

Journal article
Published: 01 June 2016 in Chemosphere
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Highlights•Four of seven MDD marker genes are transcribed in HUVEC and HT29 cells.•MDD marker genes transcription is affected by very low ethanol concentrations.•BPA levels found in humans alter transcription patterns of MDD marker genes. AbstractBisphenol A (BPA) is capable of mimicking endogenous hormones with potential consequences for human health and BPA exposure has been associated with several human diseases including neuropsychiatric disorders. Here, quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) results show that BPA at low concentrations (10 ng/mL and 1 μg/mL) induces differential transcript levels of four biomarker genes for Major Depressive Disorder (MDD) in HT29 human colon adenocarcinona cell line and Human Umbilical Vein Endothelial Cells (HUVEC). These results substantiate increasing concerns of BPA exposure in levels currently detected in humans.

ACS Style

Edna Ribeiro-Varandas; H. Sofia Pereira; Wanda Viegas; Margarida Delgado. Bisphenol A alters transcript levels of biomarker genes for Major Depressive Disorder in vascular endothelial cells and colon cancer cells. Chemosphere 2016, 153, 75 -77.

AMA Style

Edna Ribeiro-Varandas, H. Sofia Pereira, Wanda Viegas, Margarida Delgado. Bisphenol A alters transcript levels of biomarker genes for Major Depressive Disorder in vascular endothelial cells and colon cancer cells. Chemosphere. 2016; 153 ():75-77.

Chicago/Turabian Style

Edna Ribeiro-Varandas; H. Sofia Pereira; Wanda Viegas; Margarida Delgado. 2016. "Bisphenol A alters transcript levels of biomarker genes for Major Depressive Disorder in vascular endothelial cells and colon cancer cells." Chemosphere 153, no. : 75-77.

Journal article
Published: 01 December 2015 in Toxicology in Vitro
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Human exposure to Bisphenol A (BPA) results mainly from ingestion of food and beverages. Information regarding BPA effects on colon cancer, one of the major causes of death in developed countries, is still scarce. Likewise, little is known about BPA drug interactions although its potential role in doxorubicin (DOX) chemoresistance has been suggested. This study aims to assess potential interactions between BPA and DOX on HT29 colon cancer cells. HT29 cell response was evaluated after exposure to BPA, DOX, or co-exposure to both chemicals. Transcriptional analysis of several cancer-associated genes (c-fos, AURKA, p21, bcl-xl and CLU) shows that BPA exposure induces slight up-regulation exclusively of bcl-xl without affecting cell viability. On the other hand, a sub-therapeutic DOX concentration (40nM) results in highly altered c-fos, bcl-xl, and CLU transcript levels, and this is not affected by co-exposure with BPA. Conversely, DOX at a therapeutic concentration (4μM) results in distinct and very severe transcriptional alterations of c-fos, AURKA, p21 and CLU that are counteracted by co-exposure with BPA resulting in transcript levels similar to those of control. Co-exposure with BPA slightly decreases apoptosis in relation to DOX 4μM alone without affecting DOX-induced loss of cell viability. These results suggest that BPA exposure can influence chemotherapy outcomes and therefore emphasize the necessity of a better understanding of BPA interactions with chemotherapeutic agents in the context of risk assessment

ACS Style

Margarida Delgado; Edna Ribeiro-Varandas. Bisphenol A at the reference level counteracts doxorubicin transcriptional effects on cancer related genes in HT29 cells. Toxicology in Vitro 2015, 29, 2009 -2014.

AMA Style

Margarida Delgado, Edna Ribeiro-Varandas. Bisphenol A at the reference level counteracts doxorubicin transcriptional effects on cancer related genes in HT29 cells. Toxicology in Vitro. 2015; 29 (8):2009-2014.

Chicago/Turabian Style

Margarida Delgado; Edna Ribeiro-Varandas. 2015. "Bisphenol A at the reference level counteracts doxorubicin transcriptional effects on cancer related genes in HT29 cells." Toxicology in Vitro 29, no. 8: 2009-2014.

Journal article
Published: 09 September 2014 in International Journal of Molecular Sciences
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Bisphenol A (BPA) is a widely utilized endocrine disruptor capable of mimicking endogenous hormones, employed in the manufacture of numerous consumer products, thereby interfering with physiological cellular functions. Recent research has shown that BPA alters epigenetic cellular mechanisms in mammals and may be correlated to enhanced cellular senescence. Here, the effects of BPA at 10 ng/mL and 1 µg/mL, concentrations found in human samples, were analyzed on HT29 human colon adenocarcinona cell line and Human Umbilical Vein Endothelial Cells (HUVEC). Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) transcriptional analysis of the Long Interspersed Element-1 (LINE-1) retroelement showed that BPA induces global transcription deregulation in both cell lines, although with more pronounced effects in HUVEC cells. Whereas there was an increase in global transcription in HT29 exclusively after 24 h of exposure, this chemical had prolonged effects on HUVEC. Immunoblotting revealed that this was not accompanied by alterations in the overall content of H3K9me2 and H3K4me3 epigenetic marks. Importantly, cell viability assays and transcriptional analysis indicated that prolonged BPA exposure affects aging processes in senescent HUVEC. To our knowledge this is the first report that BPA interferes with senescence in primary vascular endothelial cells, therefore, suggesting its association to the etiology of age-related human pathologies, such as atherosclerosis.

ACS Style

Edna Ribeiro-Varandas; H. Sofia Pereira; Sara Monteiro; Elsa Neves; Luisa Brito; Ricardo Boavida Ferreira; Wanda Viegas; Margarida Delgado. Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells. International Journal of Molecular Sciences 2014, 15, 15791 -15805.

AMA Style

Edna Ribeiro-Varandas, H. Sofia Pereira, Sara Monteiro, Elsa Neves, Luisa Brito, Ricardo Boavida Ferreira, Wanda Viegas, Margarida Delgado. Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells. International Journal of Molecular Sciences. 2014; 15 (9):15791-15805.

Chicago/Turabian Style

Edna Ribeiro-Varandas; H. Sofia Pereira; Sara Monteiro; Elsa Neves; Luisa Brito; Ricardo Boavida Ferreira; Wanda Viegas; Margarida Delgado. 2014. "Bisphenol A Disrupts Transcription and Decreases Viability in Aging Vascular Endothelial Cells." International Journal of Molecular Sciences 15, no. 9: 15791-15805.