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Aflatoxin B1 is a mycotoxin that widely exists in feed and has a great impact on human and animal health. This study aimed to examine whether Bacillus amyloliquefaciens B10 protected against aflatoxin B1-induced cecal inflammation in mice. It was found that Bacillus amyloliquefaciens B10 could significantly improve the effects of AFB1 on body weight and intestinal inflammation of mice and enhance the expression of tight-junction protein. Compared with the CON group, the combination of AFB1 and B10 significantly increased the abundance of Actinobacteria and Bacilli in a collaborative manner, and significantly reduced the abundance of Ruminococcae, Lactobacillaceae and Clostridia. Meanwhile, the results showed that the abundance of Bacterides and Bacterdia in AFB1 + B10 group was significantly lower than that of AFB1 group, and the Firmicutes increased significantly. Bacillus amyloliquefaciens B10 can be used as a feed additive and alleviate cecal inflammation induced by AFB1 in mice by regulating intestinal flora.
Jia Chen; Zhiming Lv; Ziyang Cheng; Tiancheng Wang; Peng Li; Aibo Wu; Eugenie Nepovimova; Miao Long; Wenda Wu; Kamil Kuca. Bacillus amyloliquefaciens B10 inhibits aflatoxin B1-induced cecal inflammation in mice by regulating their intestinal flora. Food and Chemical Toxicology 2021, 156, 112438 .
AMA StyleJia Chen, Zhiming Lv, Ziyang Cheng, Tiancheng Wang, Peng Li, Aibo Wu, Eugenie Nepovimova, Miao Long, Wenda Wu, Kamil Kuca. Bacillus amyloliquefaciens B10 inhibits aflatoxin B1-induced cecal inflammation in mice by regulating their intestinal flora. Food and Chemical Toxicology. 2021; 156 ():112438.
Chicago/Turabian StyleJia Chen; Zhiming Lv; Ziyang Cheng; Tiancheng Wang; Peng Li; Aibo Wu; Eugenie Nepovimova; Miao Long; Wenda Wu; Kamil Kuca. 2021. "Bacillus amyloliquefaciens B10 inhibits aflatoxin B1-induced cecal inflammation in mice by regulating their intestinal flora." Food and Chemical Toxicology 156, no. : 112438.
Fumonisin B1 (FB1) is a contaminant that commonly present in the global environment, especially in food and feed. Epidemiologic studies have shown that esophageal cancer is associated with fumonisin toxicity. However, the molecular mechanism of FB1-induced esophageal cancer is unclear. In this research, the molecular mechanism of FB1-induced cell carcinogenesis in human esophageal epithelial cells line (HEEC) was explored. We found that FB1 (0.3125–5 μM) could promote cell proliferation, and the same phenomenon was found in a 3D cell model. FB1 could also accelerate cell migration. The expression levels of DNA damage markers were significantly increased after FB1 exposure. Meanwhile, the expression levels of cell cycle-regulated proteins and cancer-related genes were abnormal. Furthermore, FB1 significantly upregulated the histone deacetylase (HDAC) expression and activated the phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt) signalling pathway. The HDAC inhibitor trichostatin A (TSA) could repressed FB1-promoted cell proliferation and abnormal phenomenon induced by FB1. Moreover, myriocin (ISP-1) could relieve FB1-enhanced HDAC expression and cell proliferation, which implied that ISP-1 may be used to block the fumonisin toxicity in the future. Our findings suggested that the HDAC/PI3K/Akt signalling pathway is a novel mechanism for FB1-induced cell carcinogenesis in HEEC and provided new ideas for the prevention and control of fumonisin toxicity, subsequently avoiding adverse effects on the ecosystem and human health.
Song Yu; Bingxuan Jia; Na Liu; Dianzhen Yu; Shuo Zhang; Aibo Wu. Fumonisin B1 triggers carcinogenesis via HDAC/PI3K/Akt signalling pathway in human esophageal epithelial cells. Science of The Total Environment 2021, 787, 147405 .
AMA StyleSong Yu, Bingxuan Jia, Na Liu, Dianzhen Yu, Shuo Zhang, Aibo Wu. Fumonisin B1 triggers carcinogenesis via HDAC/PI3K/Akt signalling pathway in human esophageal epithelial cells. Science of The Total Environment. 2021; 787 ():147405.
Chicago/Turabian StyleSong Yu; Bingxuan Jia; Na Liu; Dianzhen Yu; Shuo Zhang; Aibo Wu. 2021. "Fumonisin B1 triggers carcinogenesis via HDAC/PI3K/Akt signalling pathway in human esophageal epithelial cells." Science of The Total Environment 787, no. : 147405.
Aflatoxin B1 is the potential chemical contaminant of most concern during the production and storage of fermented tea. In this work, a simple, fast, sensitive, accurate, and inexpensive method has been developed and validated for the simultaneous detection of four aflatoxins in fermented tea based on a modified sample pretreatment method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). Aflatoxins were extracted using acetonitrile and purified using mixed fillers (carboxyl multiwalled carbon nanotubes, hydrophilic-lipophilic balance, silica gel). Under optimum LC-MS conditions, the limits of quantification (LOQs) were 0.02–0.5 µg·kg−1. Recoveries from aflatoxins-fortified tea samples (1–12 µg·kg−1) were in the range of 78.94–105.23% with relative standard deviations (RSDs) less than 18.20%. The proposed method was applied successfully to determine aflatoxin levels in fermented tea samples.
Haiyan Zhou; Na Liu; Zheng Yan; Dianzhen Yu; Lan Wang; Kunbo Wang; Xinlin Wei; Aibo Wu. Development and validation of the one-step purification method coupled to LC-MS/MS for simultaneous determination of four aflatoxins in fermented tea. Food Chemistry 2021, 354, 129497 .
AMA StyleHaiyan Zhou, Na Liu, Zheng Yan, Dianzhen Yu, Lan Wang, Kunbo Wang, Xinlin Wei, Aibo Wu. Development and validation of the one-step purification method coupled to LC-MS/MS for simultaneous determination of four aflatoxins in fermented tea. Food Chemistry. 2021; 354 ():129497.
Chicago/Turabian StyleHaiyan Zhou; Na Liu; Zheng Yan; Dianzhen Yu; Lan Wang; Kunbo Wang; Xinlin Wei; Aibo Wu. 2021. "Development and validation of the one-step purification method coupled to LC-MS/MS for simultaneous determination of four aflatoxins in fermented tea." Food Chemistry 354, no. : 129497.
Recently, biogenic toxins have received increasing attention owing to their high contamination levels in feed and food as well as in the environment. However, there is a lack of an integrative platform for seamless linking of data-driven computational methods with ‘wet’ experimental validations. To this end, we constructed a novel platform that integrates the technical aspects of toxin biotransformation methods. First, a biogenic toxin database termed ToxinDB (http://www.rxnfinder.org/toxindb/), containing multifaceted data on more than 4836 toxins, was built. Next, more than 8000 biotransformation reaction rules were extracted from over 300,000 biochemical reactions extracted from ~580,000 literature reports curated by more than 100 people over the past decade. Based on these reaction rules, a toxin biotransformation prediction model was constructed. Finally, the global chemical space of biogenic toxins was constructed, comprising ~550,000 toxins and putative toxin metabolites, of which 94.7% of the metabolites have not been previously reported. Additionally, we performed a case study to investigate citrinin metabolism in Trichoderma, and a novel metabolite was identified with the assistance of the biotransformation prediction tool of ToxinDB. This unique integrative platform will assist exploration of the ‘dark matter’ of a toxin’s metabolome and promote the discovery of detoxification enzymes.
Dachuan Zhang; Ye Tian; Yu Tian; Huadong Xing; Sheng Liu; Haoyang Zhang; Shaozhen Ding; Pengli Cai; Dandan Sun; Tong Zhang; Yanhong Hong; Hongkun Dai; Weizhong Tu; Junni Chen; Aibo Wu; Qian-Nan Hu. A data-driven integrative platform for computational prediction of toxin biotransformation with a case study. Journal of Hazardous Materials 2020, 408, 124810 .
AMA StyleDachuan Zhang, Ye Tian, Yu Tian, Huadong Xing, Sheng Liu, Haoyang Zhang, Shaozhen Ding, Pengli Cai, Dandan Sun, Tong Zhang, Yanhong Hong, Hongkun Dai, Weizhong Tu, Junni Chen, Aibo Wu, Qian-Nan Hu. A data-driven integrative platform for computational prediction of toxin biotransformation with a case study. Journal of Hazardous Materials. 2020; 408 ():124810.
Chicago/Turabian StyleDachuan Zhang; Ye Tian; Yu Tian; Huadong Xing; Sheng Liu; Haoyang Zhang; Shaozhen Ding; Pengli Cai; Dandan Sun; Tong Zhang; Yanhong Hong; Hongkun Dai; Weizhong Tu; Junni Chen; Aibo Wu; Qian-Nan Hu. 2020. "A data-driven integrative platform for computational prediction of toxin biotransformation with a case study." Journal of Hazardous Materials 408, no. : 124810.
Scope Fatty acid esters of 2‐monochloropropane‐1,3‐diol (2‐MCPD) and 3‐monochloropropane‐1,2‐diol (3‐MCPD) are formed during the deodorization of vegetable oils. After lipase‐catalyzed hydrolysis in the intestine, 2‐ and 3‐MCPD are absorbed, but their ensuing human metabolism is unknown. Methods and results The compounds 2‐chlorohydracrylic acid (2‐ClHA) and 3‐chlorolactic acid (3‐ClLA) resulting from oxidative metabolism of 2‐MCPD and 3‐MCPD, respectively, were identified and quantified in human urine samples. An exposure study with 12 adults was conducted to determine the urinary excretion of 2‐ClHA and 3‐ClLA. The participants ate 12 g of a hazelnut oil containing 24.2 mg/kg 2‐MCPD and 54.5 mg/kg 3‐MCPD in form of fatty acid esters. Average daily amounts of “background” excretion before the exposure were 69 nmol 2‐ClHA and 3.0 nmol 3‐ClLA. The additional mean excretion due to the uptake of the hazelnut oil amounted to 893 nmol 2‐ClHA (34.0 % of the 2‐MCPD dose) and 16.4 nmol 3‐ClLA (0.28 % of the 3‐MPCD dose). Conclusions The products of oxidative metabolism of 2‐ and 3‐MCPD, 2‐ClHA and 3‐ClLA, were described for the first time in humans. Due to the lack of specificity, the metabolites may not be used as exposure biomarkers to low doses of bound 2‐ and 3‐MCPD, respectively. This article is protected by copyright. All rights reserved
Nick Bergau; Zhiyong Zhao; Klaus Abraham; Bernhard H. Monien. Metabolites of 2‐ and 3‐Monochloropropanediol (2‐ and 3‐MCPD) in Humans: Urinary Excretion of 2‐Chlorohydracrylic Acid and 3‐Chlorolactic Acid after Controlled Exposure to a Single High Dose of Fatty Acid Esters of 2‐ and 3‐MCPD. Molecular Nutrition & Food Research 2020, 65, e2000736 .
AMA StyleNick Bergau, Zhiyong Zhao, Klaus Abraham, Bernhard H. Monien. Metabolites of 2‐ and 3‐Monochloropropanediol (2‐ and 3‐MCPD) in Humans: Urinary Excretion of 2‐Chlorohydracrylic Acid and 3‐Chlorolactic Acid after Controlled Exposure to a Single High Dose of Fatty Acid Esters of 2‐ and 3‐MCPD. Molecular Nutrition & Food Research. 2020; 65 (4):e2000736.
Chicago/Turabian StyleNick Bergau; Zhiyong Zhao; Klaus Abraham; Bernhard H. Monien. 2020. "Metabolites of 2‐ and 3‐Monochloropropanediol (2‐ and 3‐MCPD) in Humans: Urinary Excretion of 2‐Chlorohydracrylic Acid and 3‐Chlorolactic Acid after Controlled Exposure to a Single High Dose of Fatty Acid Esters of 2‐ and 3‐MCPD." Molecular Nutrition & Food Research 65, no. 4: e2000736.
Mycotoxins are toxic fungal metabolites, contaminating cereal grains in field or during processing and storage periods. These environmental contaminants pose great threats to humans and animals’ health due to their toxic effects. Type A trichothecenes, fumonisins and fusaric acid (FA) are commonly detected mycotoxins produced by various Fusarium species. Trichoderma spp. are promising antagonists in agriculture for their activities against plant pathogens, and also regarded as potential candidates for bioremediation of environmental contaminants. Managing toxigenic fungi by antagonistic Trichoderma is regarded as a sustainable and eco-friendly strategy for mycotoxin control. However, the metabolic activities of Trichoderma on natural occurring mycotoxins were less investigated. Our current work comprehensively explored the activities of Trichoderma against type A trichothecenes, fumonisins and FA producing Fusarium species via co-culture competition and indirect volatile assays. Furthermore, we investigated metabolism of type A trichothecenes and FA in Trichoderma isolates. Results indicated that Trichoderma were capable of bio-transforming T-2 toxin, HT-2 toxin, diacetoxyscirpenol and neosolaniol into their glycosylated forms and one Trichoderma strain could bio transform FA into low toxic fusarinol. These findings proved that Trichoderma isolates could manage toxigenic Fusarium via direct competition and volatile-mediated indirect inhibition. In addition, these antagonists possess defensive systems against mycotoxins for self-protection, which enriches our understanding on the interaction mechanism of Trichoderma spp. on toxigenic fungus.
Ye Tian; Dianzhen Yu; Na Liu; Yan Tang; Zheng Yan; Aibo Wu. Confrontation assays and mycotoxin treatment reveal antagonistic activities of Trichoderma and the fate of Fusarium mycotoxins in microbial interaction. Environmental Pollution 2020, 267, 115559 .
AMA StyleYe Tian, Dianzhen Yu, Na Liu, Yan Tang, Zheng Yan, Aibo Wu. Confrontation assays and mycotoxin treatment reveal antagonistic activities of Trichoderma and the fate of Fusarium mycotoxins in microbial interaction. Environmental Pollution. 2020; 267 ():115559.
Chicago/Turabian StyleYe Tian; Dianzhen Yu; Na Liu; Yan Tang; Zheng Yan; Aibo Wu. 2020. "Confrontation assays and mycotoxin treatment reveal antagonistic activities of Trichoderma and the fate of Fusarium mycotoxins in microbial interaction." Environmental Pollution 267, no. : 115559.
Fusarium mycotoxins contaminated in maize are of serious threat to food security and safety. The main abiotic factors of mycotoxins-producing strains and circumstantial elements are crucial for mycotoxin production in the cereal matrix. In this current research work, Fusarium verticillioides and Fusarium graminearum are selected, which enable to produce the major mycotoxins in maize as common Fusarium isolates infecting maize. We first confirmed the constitution of mycotoxin profiles in 110 maize and derived feed samples collected from various provinces of China, through large-scale screening by the established high performance liquid chromatography-mass spectrometry (LC-MS/MS) approach. As indicated, the majority of mycotoxins was fumonisin, deoxynivalenol and zearalenone, frequently co-occurred with at least two kinds of mycotoxins. Interestingly, zearalenone-14-sulfate together with other masked forms of the target Fusarium mycotoxins were as co-contaminants there. Moreover, the circumstantial effects from a set of temperature and water activity (aw) are thoroughly demonstrated for potential contamination in maize flour and kernels. As revealed, the highest group factors were 0.99 and 25 °C while the relatively lower group factors were of temperature ≤20 °C or aw ≤ 0.95. Finally, a response surface analysis was performed to display the interaction of temperature-water activity interaction, required for potential contamination of Fusarium mycotoxins in maize and derived products. The risk group factors predicted by response surface equation were similar to the experimental results. This also could be valuable clues for pre-warning of mycotoxins occurrence, thus as reference data for control of mycotoxins in foods.
Song Yu; Bingxuan Jia; Kailin Li; Haiyan Zhou; Wenshan Lai; Yan Tang; Zheng Yan; Wei Sun; Na Liu; Dianzhen Yu; Aibo Wu. Pre-warning of abiotic factors in maize required for potential contamination of fusarium mycotoxins via response surface analysis. Food Control 2020, 121, 107570 .
AMA StyleSong Yu, Bingxuan Jia, Kailin Li, Haiyan Zhou, Wenshan Lai, Yan Tang, Zheng Yan, Wei Sun, Na Liu, Dianzhen Yu, Aibo Wu. Pre-warning of abiotic factors in maize required for potential contamination of fusarium mycotoxins via response surface analysis. Food Control. 2020; 121 ():107570.
Chicago/Turabian StyleSong Yu; Bingxuan Jia; Kailin Li; Haiyan Zhou; Wenshan Lai; Yan Tang; Zheng Yan; Wei Sun; Na Liu; Dianzhen Yu; Aibo Wu. 2020. "Pre-warning of abiotic factors in maize required for potential contamination of fusarium mycotoxins via response surface analysis." Food Control 121, no. : 107570.
Fumonisin contaminates food and feed extensively throughout the world, causing chronic and acute toxicity in human and animals. Currently, studies on the toxicology of fumonisins mainly focus on fumonisin B1 (FB1). Considering that FB1, fumonisin B2 (FB2) and fumonisin B3 (FB3) could coexist in food and feed, a study regarding a single toxin, FB1, may not completely reflect the toxicity of fumonisin. The gastrointestinal tract is usually exposed to these dietary toxins. In our study, the human gastric epithelial cell line (GES-1) was used as in vitro model to evaluate the toxicity of fumonisin. Firstly, we found that they could cause a decrease in cell viability, and increase in membrane leakage, cell death and the induction of expression of markers for endoplasmic reticulum (ER) stress. Their toxicity potency rank is FB1 > FB2 >> FB3. The results also showed that the synergistic effect appeared in the combinations of FB1 + FB2 and FB1 + FB3. Nevertheless, the combinations of FB2 + FB3 and FB1 + FB2 + FB3 showed a synergistic effect at low concentration and an antagonistic effect at high concentration. We also found that myriocin (ISP-1) could alleviate the cytotoxicity induced by fumonisin in GES-1 cells. Finally, this study may help to determine or optimize the legal limits and risk assessment method of mycotoxins in food and feed and provide a potential method to block the fumonisin toxicity.
Song Yu; Bingxuan Jia; Na Liu; Dianzhen Yu; Aibo Wu. Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells. International Journal of Molecular Sciences 2020, 21, 5917 .
AMA StyleSong Yu, Bingxuan Jia, Na Liu, Dianzhen Yu, Aibo Wu. Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells. International Journal of Molecular Sciences. 2020; 21 (16):5917.
Chicago/Turabian StyleSong Yu; Bingxuan Jia; Na Liu; Dianzhen Yu; Aibo Wu. 2020. "Evaluation of the Individual and Combined Toxicity of Fumonisin Mycotoxins in Human Gastric Epithelial Cells." International Journal of Molecular Sciences 21, no. 16: 5917.
Trichothecenes are the most common mycotoxins contaminating small grain cereals worldwide. The C12,13 epoxide group in the trichothecenes was identified as a toxic group posing harm to humans, farm animals, and plants. Aerobic biological de-epoxidation is considered the ideal method of controlling these types of mycotoxins. In this study, we isolated a novel trichothecene mycotoxin-de-epoxidating bacterium, Desulfitobacterium sp. PGC-3-9, from a consortium obtained from the soil of a wheat field known for the occurrence of frequent Fusarium head blight epidemics under aerobic conditions. Along with MMYPF media, a combination of two antibiotics (sulfadiazine and trimethoprim) substantially increased the relative abundance of Desulfitobacterium species from 1.55% (aerobic) to 29.11% (aerobic) and 28.63% (anaerobic). A single colony purified strain, PGC-3-9, was isolated and a 16S rRNA sequencing analysis determined that it was Desulfitobacterium. The PGC-3-9 strain completely de-epoxidated HT-2, deoxynivalenol (DON), nivalenol and 15-acetyl deoxynivalenol, and efficiently eliminated DON in wheat grains under aerobic and anaerobic conditions. The strain PGC-3-9 exhibited high DON de-epoxidation activity at a wide range of pH (6–10) and temperature (15–50 °C) values under both conditions. This strain may be used for the development of detoxification agents in the agriculture and feed industries and the isolation of de-epoxidation enzymes.
Wei-Jie He; Meng-Meng Shi; Peng Yang; Tao Huang; Qing-Song Yuan; Shu-Yuan Yi; Ai-Bo Wu; He-Ping Li; Chun-Bao Gao; Jing-Bo Zhang; Yu-Cai Liao. Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions. Toxins 2020, 12, 363 .
AMA StyleWei-Jie He, Meng-Meng Shi, Peng Yang, Tao Huang, Qing-Song Yuan, Shu-Yuan Yi, Ai-Bo Wu, He-Ping Li, Chun-Bao Gao, Jing-Bo Zhang, Yu-Cai Liao. Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions. Toxins. 2020; 12 (6):363.
Chicago/Turabian StyleWei-Jie He; Meng-Meng Shi; Peng Yang; Tao Huang; Qing-Song Yuan; Shu-Yuan Yi; Ai-Bo Wu; He-Ping Li; Chun-Bao Gao; Jing-Bo Zhang; Yu-Cai Liao. 2020. "Novel Soil Bacterium Strain Desulfitobacterium sp. PGC-3-9 Detoxifies Trichothecene Mycotoxins in Wheat via De-Epoxidation under Aerobic and Anaerobic Conditions." Toxins 12, no. 6: 363.
Among the family of mycotoxins of deoxynivalenol (DON) detected in nature, high proportions of 15-acetyldeoxynivalenol (15ADON) co-occur with the prototype DON and increase the combined exposure and synergistic health risks. The current study aimed to explore the mechanisms underlying the toxicity of 15ADON and compare them with those of DON. As the natural flavonoid compound quercetin (QUE) possesses antioxidant properties, we also aimed to determine the antioxidant effects of QUE on the tested mycotoxins. First, the global metabolomics approach was applied and showed that the metabolites produced from 15ADON or DON were almost identical, while QUE reversed the changes in the levels of key metabolites. Specifically, both DON and 15ADON activated the cell apoptosis pathway mediated by p38 and JNK, but inhibited the cell survival pathway mediated by ERK1/2 in GES-1 cells. Simultaneously, 15ADON induced FOXO3a nuclear translocation, similar to the results described for DON in our recent report. Furthermore, the addition of QUE appeared to counteract the detrimental effects of 15ADON and DON. We observed the effects of QUE treatment on mutant yeast strains with defects in their antioxidant system. More interestingly, QUE also substantially restored the increased ROS levels and the inhibited the growth rate following exposure to the mycotoxins DON and 15ADON. The data reported here support the hypothesis that QUE rescues the toxic effects of DON or 15ADON due to the similar mechanisms of DON and 15ADON toxicity.
Y.X. Yang; S. Yu; B.X. Jia; Na Liu; Aibo Wu. Metabolomic profiling reveals similar cytotoxic effects and protective functions of quercetin during deoxynivalenol- and 15-acetyl deoxynivalenol-induced cell apoptosis. Toxicology in Vitro 2020, 66, 104838 .
AMA StyleY.X. Yang, S. Yu, B.X. Jia, Na Liu, Aibo Wu. Metabolomic profiling reveals similar cytotoxic effects and protective functions of quercetin during deoxynivalenol- and 15-acetyl deoxynivalenol-induced cell apoptosis. Toxicology in Vitro. 2020; 66 ():104838.
Chicago/Turabian StyleY.X. Yang; S. Yu; B.X. Jia; Na Liu; Aibo Wu. 2020. "Metabolomic profiling reveals similar cytotoxic effects and protective functions of quercetin during deoxynivalenol- and 15-acetyl deoxynivalenol-induced cell apoptosis." Toxicology in Vitro 66, no. : 104838.
The Fusarium mycotoxin deoxynivalenol (DON) is typically controlled by fungicides. Here, we report DON detoxification using enzymes from the highly active Devosia strain D6-9 which degraded DON at 2.5 μg/min/108 cells. Strain D6-9 catabolized DON to 3-keto-DON and 3-epi-DON, completely removing DON in wheat. Genome analysis of three Devosia strains (D6-9, D17, and D13584), with strain D6-9 transcriptomes, identified three genes responsible for DON epimerization. One gene encodes a quinone-dependent DON dehydrogenase QDDH which oxidized DON into 3-keto-DON. Two genes encode the NADPH-dependent aldo/keto reductases AKR13B2 and AKR6D1 that convert 3-keto-DON into 3-epi-DON. Recombinant proteins expressed in Escherichia coli efficiently degraded DON in wheat grains. Molecular docking and site-directed mutagenesis revealed that residues S497, E499, and E535 function in QDDH's DON-oxidizing activity. These results advance potential microbial and enzymatic elimination of DON in agricultural samples and lend insight into the underlying mechanisms and molecular evolution of DON detoxification.
Wei-Jie He; Meng-Meng Shi; Peng Yang; Tao Huang; Yue Zhao; Ai-Bo Wu; Wu-Bei Dong; He-Ping Li; Jing-Bo Zhang; Yu-Cai Liao. A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain. Food Chemistry 2020, 321, 126703 .
AMA StyleWei-Jie He, Meng-Meng Shi, Peng Yang, Tao Huang, Yue Zhao, Ai-Bo Wu, Wu-Bei Dong, He-Ping Li, Jing-Bo Zhang, Yu-Cai Liao. A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain. Food Chemistry. 2020; 321 ():126703.
Chicago/Turabian StyleWei-Jie He; Meng-Meng Shi; Peng Yang; Tao Huang; Yue Zhao; Ai-Bo Wu; Wu-Bei Dong; He-Ping Li; Jing-Bo Zhang; Yu-Cai Liao. 2020. "A quinone-dependent dehydrogenase and two NADPH-dependent aldo/keto reductases detoxify deoxynivalenol in wheat via epimerization in a Devosia strain." Food Chemistry 321, no. : 126703.
The presence of natural toxins, pesticide residues, and illegal additives in food products has been associated with a range of potential health hazards. However, no systematic database exists that comprehensively includes and integrates all research information on these compounds, and valuable information remains scattered across numerous databases and extensive literature reports. Thus, using natural language processing technology, we curated 12,018 food risk components from 152,737 literature reports, 12 authoritative databases, and numerous related regulatory documents. Data on molecular structures, physicochemical properties, chemical taxonomy, absorption, distribution, metabolism, excretion, toxicity properties, and physiological targets within the human body were integrated to afford the comprehensive food risk component database (FRCD, http://www.rxnfinder.org/frcd/). We also analyzed the molecular scaffold and chemical diversity, in addition to evaluating the toxicity and biodegradability of the food risk components. The FRCD could be considered a highly promising tool for future food safety studies.
Dachuan Zhang; Linlin Gong; Shaozhen Ding; Ye Tian; Cancan Jia; Dongliang Liu; Mengying Han; Xingxiang Cheng; Dandan Sun; Pengli Cai; Yu Tian; Le Yuan; Weizhong Tu; Junni Chen; Aibo Wu; Qian-Nan Hu. FRCD: A comprehensive food risk component database with molecular scaffold, chemical diversity, toxicity, and biodegradability analysis. Food Chemistry 2020, 318, 126470 .
AMA StyleDachuan Zhang, Linlin Gong, Shaozhen Ding, Ye Tian, Cancan Jia, Dongliang Liu, Mengying Han, Xingxiang Cheng, Dandan Sun, Pengli Cai, Yu Tian, Le Yuan, Weizhong Tu, Junni Chen, Aibo Wu, Qian-Nan Hu. FRCD: A comprehensive food risk component database with molecular scaffold, chemical diversity, toxicity, and biodegradability analysis. Food Chemistry. 2020; 318 ():126470.
Chicago/Turabian StyleDachuan Zhang; Linlin Gong; Shaozhen Ding; Ye Tian; Cancan Jia; Dongliang Liu; Mengying Han; Xingxiang Cheng; Dandan Sun; Pengli Cai; Yu Tian; Le Yuan; Weizhong Tu; Junni Chen; Aibo Wu; Qian-Nan Hu. 2020. "FRCD: A comprehensive food risk component database with molecular scaffold, chemical diversity, toxicity, and biodegradability analysis." Food Chemistry 318, no. : 126470.
Fumonisin B1 (FB1) is a mycotoxin, produced by Fusarium verticillioides and Fusarium proliferatum, and a common fungal contaminant of maize worldwide. Its potential health hazard as a natural toxin is well documented in human and domestic animals. However, the molecular mechanism and the key factors responsible for FB1-induced cytotoxicity have not been elucidated. In this study, we first examined the cytotoxicity induced by FB1 in human gastric epithelial cell line (GES-1). We found that FB1 notably decreased cell viability and induced apoptotic cell death. Furthermore, the levels of ER stress markers were significantly increased after FB1 exposure and the ER stress inhibitor 4-phenylbutyric acid strongly suppressed FB1-induced cytotoxicity. Interestingly, the inhibition of PERK activity by GSK2606414 or shPERK3 blocked FB1-induced apoptotic cell death and cell proliferation suppression, which indicated that the cytotoxicity induced by FB1 was dependent on this signalling pathway. Moreover, myriocin could relieve FB1-induced ER stress and prevent cell death, which implied that the disruption of sphingolipid metabolism is an apical event for FB1-induced cytotoxicity. In the present study, we demonstrated that the ER stress-related PERK-CHOP signalling pathway is a novel mechanism for FB1-induced cytotoxicity and the gastrointestinal injury caused by FB1 should be concerned in the future.
Song Yu; Bingxuan Jia; Yunxia Yang; Na Liu; Aibo Wu. Involvement of PERK-CHOP pathway in fumonisin B1- induced cytotoxicity in human gastric epithelial cells. Food and Chemical Toxicology 2019, 136, 111080 .
AMA StyleSong Yu, Bingxuan Jia, Yunxia Yang, Na Liu, Aibo Wu. Involvement of PERK-CHOP pathway in fumonisin B1- induced cytotoxicity in human gastric epithelial cells. Food and Chemical Toxicology. 2019; 136 ():111080.
Chicago/Turabian StyleSong Yu; Bingxuan Jia; Yunxia Yang; Na Liu; Aibo Wu. 2019. "Involvement of PERK-CHOP pathway in fumonisin B1- induced cytotoxicity in human gastric epithelial cells." Food and Chemical Toxicology 136, no. : 111080.
Apart from causing serious yield losses, various kinds of mycotoxins may be accumulated in plant tissues infected by Fusarium strains. Fusarium mycotoxin contamination is one of the most important concerns in the food safety field nowadays. However, limited information on the causal agents, etiology, and mycotoxin production of this disease is available on pepper in China. This research was conducted to identify the Fusarium species causing pepper fruit rot and analyze their toxigenic potential in China. Forty-two Fusarium strains obtained from diseased pepper from six provinces were identified as F. equiseti (27 strains), F. solani (10 strains), F. fujikuroi (five strains). This is the first report of F. equiseti, F. solani and F. fujikuroi associated with pepper fruit rot in China, which revealed that the population structure of Fusarium species in this study was quite different from those surveyed in other countries, such as Canada and Belgium. The mycotoxin production capabilities were assessed using a well-established liquid chromatography mass spectrometry method. Out of the thirty-six target mycotoxins, fumonisins B1 and B2, fusaric acid, beauvericin, moniliformin, and nivalenol were detected in pepper tissues. Furthermore, some mycotoxins were found in non-colonized parts of sweet pepper fruit, implying migration from colonized to non-colonized parts of pepper tissues, which implied the risk of mycotoxin contamination in non-infected parts of food products.
Jianhua Wang; Shuangxia Wang; Zhiyong Zhao; Shanhai Lin; François Van Hove; Aibo Wu; Wang; Zhao; Lin; Van Hove; Wu. Species Composition and Toxigenic Potential of Fusarium Isolates Causing Fruit Rot of Sweet Pepper in China. Toxins 2019, 11, 690 .
AMA StyleJianhua Wang, Shuangxia Wang, Zhiyong Zhao, Shanhai Lin, François Van Hove, Aibo Wu, Wang, Zhao, Lin, Van Hove, Wu. Species Composition and Toxigenic Potential of Fusarium Isolates Causing Fruit Rot of Sweet Pepper in China. Toxins. 2019; 11 (12):690.
Chicago/Turabian StyleJianhua Wang; Shuangxia Wang; Zhiyong Zhao; Shanhai Lin; François Van Hove; Aibo Wu; Wang; Zhao; Lin; Van Hove; Wu. 2019. "Species Composition and Toxigenic Potential of Fusarium Isolates Causing Fruit Rot of Sweet Pepper in China." Toxins 11, no. 12: 690.
The co-occurrence of food contaminants has caught the public concern worldwide owing to their extensive distribution and the potential risk on human health. In this study, a total of 6471 samples of cereals and cereal-based products was collected from Shanghai between 2008 and 2011. The food contaminants co-occurred, including mycotoxins (aflatoxin B1, deoxynivalenol and ochratoxin A), pesticides (organic phosphorus pesticides and methyl bromide), heavy metals (cadmium, lead, and inorganic arsenic), and food additives (aluminum and food pigments), were analyzed for their contents. The point evaluation and probabilistic evaluation based on the Monte Carlo model were performed to assess the risks of the contaminants. The results showed the mean levels of all the tested contaminants were lower than the maximum residue levels (MRLs) set by China, except aluminum, exceeding China MRLs of 100 mg/kg in 2010 and 2011. The point evaluation showed the mean daily exposures (40.7–63.9 ng/kg bw/day) of ochratoxin A in 2010 for different consumer groups were higher than the provisional tolerable daily intake (PTDI) of 17 ng/kg bw/day recommended by the European Food Safety Authority (EFSA). Meanwhile, the mean daily exposures of the tested contaminants in each year for 7-to-10-year old children were higher than those for adults. The probabilistic evaluation indicated that the margin of safety (MOS) values of the tested contaminants at percentile 95th (P95) were lower than 1, except aluminum, suggesting the potential health risk of aluminum intake to the consumer groups in Shanghai. Additionally, the risk ranking of these contaminants in cereals and cereal-based products was obtained as follows: aluminum > deoxynivalenol > ochratoxin A > inorganic arsenic > cadmium > lead > chlorpyrifos-methyl > malathion.
Xianli Yang; Zhiyong Zhao; Yanglan Tan; Bo Chen; Changyan Zhou; Aibo Wu. Risk profiling of exposures to multiclass contaminants through cereals and cereal-based products consumption: A case study for the inhabitants in Shanghai, China. Food Control 2019, 109, 106964 .
AMA StyleXianli Yang, Zhiyong Zhao, Yanglan Tan, Bo Chen, Changyan Zhou, Aibo Wu. Risk profiling of exposures to multiclass contaminants through cereals and cereal-based products consumption: A case study for the inhabitants in Shanghai, China. Food Control. 2019; 109 ():106964.
Chicago/Turabian StyleXianli Yang; Zhiyong Zhao; Yanglan Tan; Bo Chen; Changyan Zhou; Aibo Wu. 2019. "Risk profiling of exposures to multiclass contaminants through cereals and cereal-based products consumption: A case study for the inhabitants in Shanghai, China." Food Control 109, no. : 106964.
Selenium nanoparticles (SNP) were generally utilized as chemotherapeutic agents and environmental ameliorants, but without simultaneous biocontrol functionalities against fungal pathogens and mycotoxins, plus unknown recognition mechanism. Selenium nanoparticles derived from Trichoderma harzianum JF309 (TSNP) were herein synthesized, contrast to traditional SNP, where diverse metabolites co-occurred in TSNP including organic acids and their derivates, such as psoromic acid, β-decyloxybenzoic acid, glucaric acid lactone, etc., revealed by Triple TOF UPLC/HRMS. As hypothesized, this interaction of diverse recognition metabolites and selenium nanoparticles in TSNP might change the arrangement and movement of original state, form random, nondirective to regulated, directional, thus contributing to better antifungal effects and dramatically increasing control functionalities against Alternaria toxins (83% of TeA and 79% of AOH reduction), fumonisin B1 (63% of FB1 reduction) and deoxynivalenol (76% of DON reduction), respectively. Meanwhile, expression of key biosynthetic genes of FUM1, PA, TRI5 and TRI6 were substantially decreased. Finally, as convinced, the obtained nanoparticles had no significant side effects to the selected three human cells. In total, the achieved data supports the biogenic nanoparticles as valuable functional materials with great potential for practical plant protection and food safety prevention.
Dongqiang Hu; Song Yu; Dianzhen Yu; Na Liu; Yan Tang; Yingying Fan; Cheng Wang; Aibo Wu. Biogenic Trichoderma harzianum-derived selenium nanoparticles with control functionalities originating from diverse recognition metabolites against phytopathogens and mycotoxins. Food Control 2019, 106, 106748 .
AMA StyleDongqiang Hu, Song Yu, Dianzhen Yu, Na Liu, Yan Tang, Yingying Fan, Cheng Wang, Aibo Wu. Biogenic Trichoderma harzianum-derived selenium nanoparticles with control functionalities originating from diverse recognition metabolites against phytopathogens and mycotoxins. Food Control. 2019; 106 ():106748.
Chicago/Turabian StyleDongqiang Hu; Song Yu; Dianzhen Yu; Na Liu; Yan Tang; Yingying Fan; Cheng Wang; Aibo Wu. 2019. "Biogenic Trichoderma harzianum-derived selenium nanoparticles with control functionalities originating from diverse recognition metabolites against phytopathogens and mycotoxins." Food Control 106, no. : 106748.
Objective: To investigate the levels of a deoxynivalenol (DON) biomarker in the urine of subjects living in two China provinces with different geographic locations and dietary patterns, and estimate their dietary DON exposures and health risks. Methods: First morning urine samples were collected on three consecutive days from 599 healthy subjects—301 from Henan province and 298 from Sichuan province—to analyze the total DON concentrations (tDON) after β-glucuronidase hydrolysis using a high-performance liquid chromatography tandem mass spectrometry-based method. The consumption of cereal foods in the previous 24 h before each urine collection was recorded using a duplicate diet method. DON exposure levels were estimated based on the urinary tDON concentrations. Results: Total DON were detected in 100% and 92% of the urine samples from Henan and Sichuan, respectively. Mean urinary tDON concentrations were 52.83 ng/mL in Henan subjects and 12.99 ng/mL in Sichuan subjects, respectively. The tDON levels were significantly higher in the urine of Henan subjects than that of the Sichuan subjects (p < 0.001). Urinary tDON levels were significantly different among age groups in both areas (Henan: p < 0.001; Sichuan: p = 0.026) and were highest in adolescents aged 13–17 years, followed by children aged 7–12 years. Based on the DON biomarker and exposure conversion reported by the European Food Safety Authority (EFSA), the mean estimated dietary intakes of DON were 1.82 μg/kg bw/day in Henan subjects and 0.45 μg/kg bw/day in Sichuan subjects. A total of 56% of Henan subjects and 12% of Sichuan subjects were estimated to exceed the PMTDI of 1 μg/kg bw/day. Consistent with urinary tDON levels, the highest estimated dietary DON intakes were also in children and adolescents aged 7–17 years. For all kinds of wheat-based foods except dumplings, the consumptions were significantly higher in Henan than those in Sichuan. The mean consumption of steamed buns was 8.4-fold higher in Henan (70.67 g/d) than that in Sichuan (8.45 g/d). The mean consumption of noodles in Henan (273.91 g/d) was 3.6-fold higher than that in Sichuan (75.87 g/d). Conclusions: The levels of urinary DON biomarker and the estimated dietary DON intakes in Henan province were high and concerning, especially for children and adolescents. The overall exposure level of Sichuan inhabitants was low.
Xiaodan Wang; Jiang Liang; Pei Cao; Shuang Zhou; Aibo Wu; Peng Gao; Haibin Xu; Zhaoping Liu; Yunyun Gong. Biomonitoring Study of Deoxynivalenol Exposure in Chinese Inhabitants. International Journal of Environmental Research and Public Health 2019, 16, 2169 .
AMA StyleXiaodan Wang, Jiang Liang, Pei Cao, Shuang Zhou, Aibo Wu, Peng Gao, Haibin Xu, Zhaoping Liu, Yunyun Gong. Biomonitoring Study of Deoxynivalenol Exposure in Chinese Inhabitants. International Journal of Environmental Research and Public Health. 2019; 16 (12):2169.
Chicago/Turabian StyleXiaodan Wang; Jiang Liang; Pei Cao; Shuang Zhou; Aibo Wu; Peng Gao; Haibin Xu; Zhaoping Liu; Yunyun Gong. 2019. "Biomonitoring Study of Deoxynivalenol Exposure in Chinese Inhabitants." International Journal of Environmental Research and Public Health 16, no. 12: 2169.
The contamination of food with Aspergillus flavus and subsequent aflatoxins is considered as one of the most severe safety problems in the world. The application of microorganisms and the produced bio-active compounds is considered as the most promising method for controlling foodborne pathogens and mycotoxins contamination both in pre- and post-harvest. Vt-7, identified as Enterobacter asburiae, was able to completely inhibit the growth of Aspergillus flavus (AF) and other seven important fungal pathogens by the production of volatiles. Additionally, it can also significantly inhibited AF infection on peanuts in storage, down-regulated the gene expression of aflatoxin biosynthesis and eventually prevented aflatoxins production. Scanning electron microscope further proved that Vt-7 volatiles prevented conidia germination of AF on peanut surface, and severely destroyed the conidia structure. Gas chromatography – tandem mass spectrometry revealed that two abundant compounds (1-Pentanol and Phenylethyl Alcohol) were involved in the volatile profiles. They showed great antagonistic activity against AF with minimal inhibitory concentration at 200 μL/L. Therefore, E. asburiae Vt-7, and volatiles 1-Pentanol and Phenylethyl Alcohol were effective agents in controlling AF and aflatoxins in peanut during storage. They will provide novel strategies for the application of microbe and bio-active compounds against fungal pathogens and mycotoxins in food and grains during storage.
An-Dong Gong; Fei-Yan Dong; Mengjun Hu; Xian-Wei Kong; Fen-Fen Wei; Shuang-Jun Gong; Yi-Mei Zhang; Jing-Bo Zhang; Ai-Bo Wu; Yu-Cai Liao. Antifungal activity of volatile emitted from Enterobacter asburiae Vt-7 against Aspergillus flavus and aflatoxins in peanuts during storage. Food Control 2019, 106, 106718 .
AMA StyleAn-Dong Gong, Fei-Yan Dong, Mengjun Hu, Xian-Wei Kong, Fen-Fen Wei, Shuang-Jun Gong, Yi-Mei Zhang, Jing-Bo Zhang, Ai-Bo Wu, Yu-Cai Liao. Antifungal activity of volatile emitted from Enterobacter asburiae Vt-7 against Aspergillus flavus and aflatoxins in peanuts during storage. Food Control. 2019; 106 ():106718.
Chicago/Turabian StyleAn-Dong Gong; Fei-Yan Dong; Mengjun Hu; Xian-Wei Kong; Fen-Fen Wei; Shuang-Jun Gong; Yi-Mei Zhang; Jing-Bo Zhang; Ai-Bo Wu; Yu-Cai Liao. 2019. "Antifungal activity of volatile emitted from Enterobacter asburiae Vt-7 against Aspergillus flavus and aflatoxins in peanuts during storage." Food Control 106, no. : 106718.
Zearalenone (ZEA) is an estrogenic and ochratoxin A (OTA) is a hepatotoxic Fusarium mycotoxin commonly seen in cereals and fruits products. No previous investigation has studied on a single platform for the multi degradation mycotoxin. The current study aimed to investigate the bifunctional activity of a novel fusion recombinant. We have generated a recombinant fusion enzyme (ZHDCP) by combining two single genes named zearalenone hydrolase (ZHD) and carboxypeptidase (CP) in frame deletion by crossover polymerase chain reaction (PCR). We identified enzymatic properties and cell cytotoxicity assay of ZHDCP enzyme. Our findings have demonstrated that ZEA was completely degraded to the non-toxic product in 2 h by ZHDCP enzyme at an optimum pH of 7 and a temperature of 35 °C. For the first time, it was found out that ZEA 60% was degraded by CP degrades in 48 h. Fusion ZHDCP and CP enzyme were able to degrade 100% OTA in 30 min at pH 7 and temperature 30 °C. ZEA- and OTA-induced cell death and increased cell apoptosis rate and regulated mRNA expression of Sirt1, Bax, Bcl2, Caspase3, TNFα, and IL6 genes. Our novel findings demonstrated that the fusion enzyme ZHDCP possess bifunctional activity (degrade OTA and ZEA), and it could be used to degrade more mycotoxins.
Shofiul Azam; Dianzhen Yu; Na Liu; Aibo Wu. Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme. Toxins 2019, 11, 301 .
AMA StyleShofiul Azam, Dianzhen Yu, Na Liu, Aibo Wu. Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme. Toxins. 2019; 11 (5):301.
Chicago/Turabian StyleShofiul Azam; Dianzhen Yu; Na Liu; Aibo Wu. 2019. "Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme." Toxins 11, no. 5: 301.
Aflatoxins (AFs), deoxynivalenols (DONs), and zearalenones (ZENs) are common mycotoxins that contaminate feedstuff, causing contamination of poultry products. In our study, these mycotoxins were quantified in 152 egg samples collected from markets in Jiangsu (JS), Zhejiang (ZJ), and Shanghai (SH) and in 70 chicken tissue samples (liver, heart, and gizzard) from ZJ in China. The main mycotoxins observed in egg samples were DON, 15-AcDON, and ZEN, although only ZEN family mycotoxins (ZEN, α-ZEL, β-ZEL, and α-ZAL) were detected in chicken tissues. Furthermore, for the first time, we assessed the health risks of exposure of three populations (children, adults, and elder adults) to DONs (DON, 3-AcDON, and 15-AcDON) and ZEN in eggs (from three different areas) and to ZEN in chicken tissues. We show that the mean dietary intake (DI) values and the 97.5th percentile DI values of DON and ZEN through egg ingestion were lower than the provisional maximum tolerable daily intake (PMTDI) (1 μg/kg body weight (BW)/day) for the three populations in the three geographical areas studied. However, eggs contaminated with high levels of DONs and ZEN contributed to a large proportion of the PMTDI of these mycotoxins, especially in children and elder adults. Although ZEN was highly detected in the chicken tissues, no significant health risk was observed.
Lan Wang; Qiaoyan Zhang; Zheng Yan; Yanglan Tan; Runyue Zhu; Dianzhen Yu; Hua Yang; Aibo Wu. Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China. Toxins 2018, 10, 477 .
AMA StyleLan Wang, Qiaoyan Zhang, Zheng Yan, Yanglan Tan, Runyue Zhu, Dianzhen Yu, Hua Yang, Aibo Wu. Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China. Toxins. 2018; 10 (11):477.
Chicago/Turabian StyleLan Wang; Qiaoyan Zhang; Zheng Yan; Yanglan Tan; Runyue Zhu; Dianzhen Yu; Hua Yang; Aibo Wu. 2018. "Occurrence and Quantitative Risk Assessment of Twelve Mycotoxins in Eggs and Chicken Tissues in China." Toxins 10, no. 11: 477.