This page has only limited features, please log in for full access.
Dinoflagellate species of the genera Gambierdiscus and Fukuyoa are known to produce ciguatera poisoning-associated toxic compounds, such as ciguatoxins, or other toxins, such as maitotoxins. However, many species and strains remain poorly characterized in areas where they were recently identified, such as the western Mediterranean Sea. In previous studies carried out by our research group, a G. australes strain from the Balearic Islands (Mediterranean Sea) presenting MTX-like activity was characterized by LC-MS/MS and LC-HRMS detecting 44-methyl gambierone and gambieric acids C and D. However, MTX1, which is typically found in some G. australes strains from the Pacific Ocean, was not detected. Therefore, this study focuses on the identification of the compound responsible for the MTX-like toxicity in this strain. The G. australes strain was characterized not only using LC-MS instruments but also N2a-guided HPLC fractionation. Following this approach, several toxic compounds were identified in three fractions by LC-MS/MS and HRMS. A novel MTX analogue, named MTX5, was identified in the most toxic fraction, and 44-methyl gambierone and gambieric acids C and D contributed to the toxicity observed in other fractions of this strain. Thus, G. australes from the Mediterranean Sea produces MTX5 instead of MTX1 in contrast to some strains of the same species from the Pacific Ocean. No CTX precursors were detected, reinforcing the complexity of the identification of CTXs precursors in these regions.
Pablo Estevez; David Castro; José Manuel Leão-Martins; Manoëlla Sibat; Angels Tudó; Robert Dickey; Jorge Diogene; Philipp Hess; Ana Gago-Martinez. Toxicity Screening of a Gambierdiscus australes Strain from the Western Mediterranean Sea and Identification of a Novel Maitotoxin Analogue. Marine Drugs 2021, 19, 460 .
AMA StylePablo Estevez, David Castro, José Manuel Leão-Martins, Manoëlla Sibat, Angels Tudó, Robert Dickey, Jorge Diogene, Philipp Hess, Ana Gago-Martinez. Toxicity Screening of a Gambierdiscus australes Strain from the Western Mediterranean Sea and Identification of a Novel Maitotoxin Analogue. Marine Drugs. 2021; 19 (8):460.
Chicago/Turabian StylePablo Estevez; David Castro; José Manuel Leão-Martins; Manoëlla Sibat; Angels Tudó; Robert Dickey; Jorge Diogene; Philipp Hess; Ana Gago-Martinez. 2021. "Toxicity Screening of a Gambierdiscus australes Strain from the Western Mediterranean Sea and Identification of a Novel Maitotoxin Analogue." Marine Drugs 19, no. 8: 460.
Ciguatera food poisoning affects consumer health and fisheries’ economies worldwide in tropical zones, and specifically in the Pacific area. The wide variety of ciguatoxins bio-accumulated in fish or shellfish responsible for this neurological illness are produced by marine dinoflagellates of the genus Gambierdiscus and bio-transformed through the food web. The evaluation of the contents of ciguatoxins in strains of Gambierdiscus relies on the availability of standards and on the development of sensitive and specific tools to detect them. There is a need for sensitive methods for the analysis of pacific ciguatoxins with high resolution mass spectrometry to ensure unequivocal identification of all congeners. We have applied a fractional factorial design of experiment 2^8-3 for the screening of the significance of eight parameters potentially influencing ionization and ion transmission and their interactions to evaluate the behavior of sodium adducts, protonated molecules and first water losses of CTX4A/B, CTX3B/C, 2-OH-CTX3C and 44-methylgambierone on a Q-TOF equipment. The four parameters that allowed to significantly increase the peak areas of ciguatoxins and gambierones (up to a factor ten) were the capillary voltage, the sheath gas temperature, the ion funnel low pressure voltage and the ion funnel exit voltage. The optimized method was applied to revisit the toxin profile of G. polynesiensis (strain TB92) with a confirmation of the presence of M-seco-CTX4A only putatively reported so far and the detection of an isomer of CTX4A. The improvement in toxin detection also allowed to obtain informative high resolution targeted MS/MS spectra revealing high similarity in fragmentation patterns between putative isomer (4) of CTX3C, 2-OH-CTX3C and CTX3B on one side and between CTX4A, M-seco-CTX4A and the putative isomer on the other side, suggesting a relation of constitutional isomerism between them for both isomers.
Thomas Yon; Manoella Sibat; Damien Réveillon; Samuel Bertrand; Mireille Chinain; Philipp Hess. Deeper insight into Gambierdiscus polynesiensis toxin production relies on specific optimization of high-performance liquid chromatography-high resolution mass spectrometry. Talanta 2021, 232, 122400 .
AMA StyleThomas Yon, Manoella Sibat, Damien Réveillon, Samuel Bertrand, Mireille Chinain, Philipp Hess. Deeper insight into Gambierdiscus polynesiensis toxin production relies on specific optimization of high-performance liquid chromatography-high resolution mass spectrometry. Talanta. 2021; 232 ():122400.
Chicago/Turabian StyleThomas Yon; Manoella Sibat; Damien Réveillon; Samuel Bertrand; Mireille Chinain; Philipp Hess. 2021. "Deeper insight into Gambierdiscus polynesiensis toxin production relies on specific optimization of high-performance liquid chromatography-high resolution mass spectrometry." Talanta 232, no. : 122400.
Some species of the genus Dinophysis contain Diarrhetic shellfish Poisoning (DSP) toxins and are the main threat to shellfish farming in Europe including France. Dinophysis species are known to produce two families of bioactive lipophilic toxins: (i) okadaic acid (OA) and their analogues dinophysistoxins (DTXs) and (ii) pectenotoxins (PTXs). Only six toxins (OA, DTX1, DTX2, DTX3, PTX1 and PTX2) regulated by the European Union Legislation (EC No. 15/2011; 3) are routinely monitored using targeted chemical analysis by liquid chromatography coupled to mass spectrometry (LC-MS/MS) while toxic species of Dinophysis produce many other analogues. To tentatively identify unknown toxin analogues, a recent approach (Molecular Networking, MN) was used based on fragmentation data obtained by untargeted high resolution mass spectrometry (HRMS). An optimization of the data-dependent LC-HRMS/MS acquisition conditions was conducted to obtain more informative networks. The MN was applied to provide an overview of the chemical diversity of four strains belonging to three major Dinophysis species isolated from French coastal waters (D. acuta, D. caudata and the “D. acuminata complex” species D. acuminata and D. sacculus). This approach highlighted species-specific chemical patterns and also that Dinophysis chemical diversity is largely unexplored. Using MN allowed to identify directly known toxins and their relationship between species of Dinophysis, leading to the discovery of five new putative PTX analogues.
Manoëlla Sibat; Damien Réveillon; Chloé Antoine; Liliane Carpentier; Georges Augustin Rovillon; Véronique Sechet; Samuel Bertrand. Molecular networking as a novel approach to unravel toxin diversity of four strains of the dominant Dinophysis species from French coastal waters. Harmful Algae 2021, 103, 102026 .
AMA StyleManoëlla Sibat, Damien Réveillon, Chloé Antoine, Liliane Carpentier, Georges Augustin Rovillon, Véronique Sechet, Samuel Bertrand. Molecular networking as a novel approach to unravel toxin diversity of four strains of the dominant Dinophysis species from French coastal waters. Harmful Algae. 2021; 103 ():102026.
Chicago/Turabian StyleManoëlla Sibat; Damien Réveillon; Chloé Antoine; Liliane Carpentier; Georges Augustin Rovillon; Véronique Sechet; Samuel Bertrand. 2021. "Molecular networking as a novel approach to unravel toxin diversity of four strains of the dominant Dinophysis species from French coastal waters." Harmful Algae 103, no. : 102026.
Dinophysis is the main dinoflagellate genus responsible for diarrheic shellfish poisoning (DSP) in human consumers of filter feeding bivalves contaminated with lipophilic diarrheic toxins. Species of this genus have a worldwide distribution driven by environmental conditions (temperature, irradiance, salinity, nutrients etc.), and these factors are sensitive to climate change. The D. acuminata-complex may contain several species, including D. sacculus. The latter has been found in estuaries and semi-enclosed areas, water bodies subjected to quick salinity variations and its natural repartition suggests some tolerance to salinity changes. However, the response of strains of D. acuminata-complex (D. cf. sacculus) subjected to salinity stress and the underlying mechanisms have never been studied in the laboratory. Here, a 24 h hypoosmotic (25) and hyperosmotic (42) stress was performed in vitro in a metabolomic study carried out with three cultivated strains of D. cf. sacculus isolated from the French Atlantic and Mediterranean coasts. Growth rate, biovolume and osmolyte (proline, glycine betaine and dimethylsulfoniopropionate (DMSP)) and toxin contents were measured. Osmolyte contents were higher at the highest salinity, but only a significant increase in glycine betaine was observed between the control (35) and the hyperosmotic treatment. Metabolomics revealed significant and strain-dependent differences in metabolite profiles for different salinities. These results, as well as the absence of effects on growth rate, biovolume, okadaic acid (OA) and pectenotoxin (PTXs) cellular contents, suggest that the D. cf. sacculus strains studied are highly tolerant to salinity variations.
Sylvain Gaillard; Damien Réveillon; Charline Danthu; Fabienne Hervé; Manoella Sibat; Liliane Carpentier; Hélène Hégaret; Véronique Séchet; Philipp Hess. Effect of a short-term salinity stress on the growth, biovolume, toxins, osmolytes and metabolite profiles on three strains of the Dinophysis acuminata-complex (Dinophysis cf. sacculus). Harmful Algae 2021, 107, 102009 .
AMA StyleSylvain Gaillard, Damien Réveillon, Charline Danthu, Fabienne Hervé, Manoella Sibat, Liliane Carpentier, Hélène Hégaret, Véronique Séchet, Philipp Hess. Effect of a short-term salinity stress on the growth, biovolume, toxins, osmolytes and metabolite profiles on three strains of the Dinophysis acuminata-complex (Dinophysis cf. sacculus). Harmful Algae. 2021; 107 ():102009.
Chicago/Turabian StyleSylvain Gaillard; Damien Réveillon; Charline Danthu; Fabienne Hervé; Manoella Sibat; Liliane Carpentier; Hélène Hégaret; Véronique Séchet; Philipp Hess. 2021. "Effect of a short-term salinity stress on the growth, biovolume, toxins, osmolytes and metabolite profiles on three strains of the Dinophysis acuminata-complex (Dinophysis cf. sacculus)." Harmful Algae 107, no. : 102009.
Dinoflagellates of the genus Dinophysis are the most prominent producers of Diarrhetic Shellfish Poisoning (DSP) toxins which have an impact on public health and on marine aquaculture worldwide. In particular, Dinophysis acuminata has been reported as the major DSP agent in Western Europe. Still, its contribution to DSP events in the regions of the English Channel and the Atlantic coast of France, and the role of the others species of the Dinophysis community in these areas are not as clear. In addition, species identification within the D. acuminata complex has proven difficult due to their highly similar morphological features. In the present study, 30 clonal strains of the dominant Dinophysis species have been isolated from French coasts including the English Channel (3 sites), the Atlantic Ocean (11 sites) and the Mediterranean Sea (6 sites). Morphologically, strains were identified as three species: D. acuta, D. caudata, D. tripos, as well as the D. acuminata-complex. Sequences of the ITS and LSU rDNA regions confirmed these identifications and revealed no genetic difference within the D. acuminata-complex. Using the mitochondrial gene cox1, two groups of strains differing by only one substitution were found in the D. acuminata-complex, but SEM analysis of various strains showed a large range of morphological variations. Based on geographical origin and morphology, strains of the subclade A were ascribed to ‘D. acuminata’ while those of the subclade B were ascribed to ‘D. sacculus’. Nevertheless, the distinction into two separate species remains questionable and was not supported by our genetic data. The considerable variations observed in cultured strains suggest that physiological factors might influence cell contour and bias identification. Analyses of Dinophysis cultures from French coastal waters using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) revealed species-conserved toxin profiles for D. acuta (dinophysistoxin 2 (DTX2), okadaic acid (OA), pectenotoxin 2 (PTX2)), D. caudata (PTX2) and D. tripos (PTX2), irrespective of geographical origin (Atlantic Ocean or Mediterranean Sea). Within the D. acuminata-complex, two different toxin profiles were observed: the strains of ‘D. acuminata’ (subclade A) from the English Channel and the Atlantic Ocean contained only OA while strains of ‘D. sacculus’ (subclade B) from Mediterranean Sea/Atlantic Ocean contained PTX2 as the dominant toxin, with OA and C9-esters also being present, albeit in lower proportions. The same difference in toxin profiles between ‘D. sacculus’ and ‘D. acuminata’ was reported in several studies from Galicia (NW- Spain). This difference in toxin profiles has consequences in terms of public health, and consequently for monitoring programs. While toxin profile could appear as a reliable feature separating ‘D. acuminata’ from ‘D. sacculus’ on both French and Spanish coasts, this does not seem consistent with observations on a broader geographical scale for the D. acuminata complex, possibly due to the frequent lack of genetic characterization.
Véronique Séchet; Manoella Sibat; Gwenael Billien; Liliane Carpentier; Georges-Augustin Rovillon; Virginie Raimbault; Florent Malo; Sylvain Gaillard; Myriam Perrière-Rumebe; Philipp Hess; Nicolas Chomérat. Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex. Harmful Algae 2021, 107, 101974 .
AMA StyleVéronique Séchet, Manoella Sibat, Gwenael Billien, Liliane Carpentier, Georges-Augustin Rovillon, Virginie Raimbault, Florent Malo, Sylvain Gaillard, Myriam Perrière-Rumebe, Philipp Hess, Nicolas Chomérat. Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex. Harmful Algae. 2021; 107 ():101974.
Chicago/Turabian StyleVéronique Séchet; Manoella Sibat; Gwenael Billien; Liliane Carpentier; Georges-Augustin Rovillon; Virginie Raimbault; Florent Malo; Sylvain Gaillard; Myriam Perrière-Rumebe; Philipp Hess; Nicolas Chomérat. 2021. "Characterization of toxin-producing strains of Dinophysis spp. (Dinophyceae) isolated from French coastal waters, with a particular focus on the D. acuminata-complex." Harmful Algae 107, no. : 101974.
Ciguatera poisoning is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. Ciguatera outbreaks are expected to increase worldwide with global change, in particular as a function of its main drivers, including changes in sea surface temperature, acidification, and coastal eutrophication. In French Polynesia, G. polynesiensis is regarded as the dominant source of CTXs entering the food web. The effects of pH (8.4, 8.2, and 7.9), Nitrogen:Phosphorus ratios (24N:1P vs. 48N:1P), and nitrogen source (nitrates vs. urea) on growth rate, biomass, CTX levels, and profiles were examined in four clones of G. polynesiensis at different culture age (D10, D21, and D30). Results highlight a decrease in growth rate and cellular biomass at low pH when urea is used as a N source. No significant effect of pH, N:P ratio, and N source on the overall CTX content was observed. Up to ten distinct analogs of Pacific ciguatoxins (P-CTXs) could be detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in clone NHA4 grown in urea, at D21. Amounts of more oxidized P-CTX analogs also increased under the lowest pH condition. These data provide interesting leads for the custom production of CTX standards.
Sébastien Longo; Manoëlla Sibat; Hélène Taiana Darius; Philipp Hess; Mireille Chinain. Effects of pH and Nutrients (Nitrogen) on Growth and Toxin Profile of the Ciguatera-Causing Dinoflagellate Gambierdiscus polynesiensis (Dinophyceae). Toxins 2020, 12, 767 .
AMA StyleSébastien Longo, Manoëlla Sibat, Hélène Taiana Darius, Philipp Hess, Mireille Chinain. Effects of pH and Nutrients (Nitrogen) on Growth and Toxin Profile of the Ciguatera-Causing Dinoflagellate Gambierdiscus polynesiensis (Dinophyceae). Toxins. 2020; 12 (12):767.
Chicago/Turabian StyleSébastien Longo; Manoëlla Sibat; Hélène Taiana Darius; Philipp Hess; Mireille Chinain. 2020. "Effects of pH and Nutrients (Nitrogen) on Growth and Toxin Profile of the Ciguatera-Causing Dinoflagellate Gambierdiscus polynesiensis (Dinophyceae)." Toxins 12, no. 12: 767.
Ciguatera poisoning (CP) results from the consumption of seafood contaminated with ciguatoxins (CTXs). This disease is highly prevalent in French Polynesia with several well-identified hotspots. Rapa Island, the southernmost inhabited island in the country, was reportedly free of CP until 2007. This study describes the integrated approach used to investigate the etiology of a fatal mass-poisoning outbreak that occurred in Rapa in 2009. Symptoms reported in patients were evocative of ciguatera. Several Gambierdiscus field samples collected from benthic assemblages tested positive by the receptor binding assay (RBA). Additionally, the toxicity screening of ≈250 fish by RBA indicated ≈78% of fish could contain CTXs. The presence of CTXs in fish was confirmed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The potential link between climate change and this range expansion of ciguatera to a subtropical locale of French Polynesia was also examined based on the analysis of temperature time-series data. Results are indicative of a global warming trend in Rapa area. A five-fold reduction in incidence rates was observed between 2009 and 2012, which was due in part to self-regulating behavior among individuals (avoidance of particular fish species and areas). Such observations underscore the prominent role played by community outreach in ciguatera risk management.
Mireille Chinain; Clémence Mahana Iti Gatti; André Ung; Philippe Cruchet; Taina Revel; Jérôme Viallon; Manoëlla Sibat; Patrick Varney; Victoire Laurent; Philipp Hess; Hélène Taiana Darius. Evidence for the Range Expansion of Ciguatera in French Polynesia: A Revisit of the 2009 Mass-Poisoning Outbreak in Rapa Island (Australes Archipelago). Toxins 2020, 12, 759 .
AMA StyleMireille Chinain, Clémence Mahana Iti Gatti, André Ung, Philippe Cruchet, Taina Revel, Jérôme Viallon, Manoëlla Sibat, Patrick Varney, Victoire Laurent, Philipp Hess, Hélène Taiana Darius. Evidence for the Range Expansion of Ciguatera in French Polynesia: A Revisit of the 2009 Mass-Poisoning Outbreak in Rapa Island (Australes Archipelago). Toxins. 2020; 12 (12):759.
Chicago/Turabian StyleMireille Chinain; Clémence Mahana Iti Gatti; André Ung; Philippe Cruchet; Taina Revel; Jérôme Viallon; Manoëlla Sibat; Patrick Varney; Victoire Laurent; Philipp Hess; Hélène Taiana Darius. 2020. "Evidence for the Range Expansion of Ciguatera in French Polynesia: A Revisit of the 2009 Mass-Poisoning Outbreak in Rapa Island (Australes Archipelago)." Toxins 12, no. 12: 759.
Rationale The dinoflagellate genera Gambierdiscus and Fukuyoa are producers of toxins responsible for Ciguatera Poisoning (CP). Although having very low oral potency, maitotoxins (MTXs) are very toxic following intraperitoneal injection and feeding studies have shown they may accumulate in fish muscle. To date, six MTX congeners have been described but two congeners (MTX2 and MTX4) have not yet been structurally elucidated. The aim of the present study was to further characterize MTX4. Methods Chemical analysis was performed using Liquid Chromatography coupled to a Diode Array Detector (DAD) and positive ionization mode High Resolution Mass Spectrometry (LC/HRMS) on partially purified extracts of G. excentricus (strain VGO792). HRMS/MS studies were also carried out to tentatively explain the fragmentation pathways of MTX and MTX4. Results The comparison of UV and HRMS (ESI+) spectra between MTX and MTX4 led us to propose the elemental formula of MTX4 (C157H241NO68S2, as unsalted molecule). The comparison of the theoretical and measured m/z values of the doubly charged ions of the isotopic profile in ESI+ were coherent with the proposed elemental formula of MTX4. The study of HRMS/MS spectra on the tri‐ammoniated adduct ([M–H+3NH4]2+) of both molecules gave additional information about structural features. The cleavage observed, probably located at C99–C100 in both MTX and MTX4, highlighted the same A‐side product ion shared by the two molecules. Conclusion All these investigations on the characterization of MTX4 contribute to highlighting that MTX4 belongs to the same structural family of MTXs. However to accomplish a complete structural elucidation of MTX4, NMR‐based study and LC/HRMSn investigation will have to be carried out.
Francesco Pisapia; Manoëlla Sibat; Ryuichi Watanabe; Catherine Roullier; Toshiyuki Suzuki; Philipp Hess; Christine Herrenknecht. Characterization of maitotoxin‐4 (MTX4) using electrospray positive mode ionization high‐resolution mass spectrometry and UV spectroscopy. Rapid Communications in Mass Spectrometry 2020, 34, e8859 .
AMA StyleFrancesco Pisapia, Manoëlla Sibat, Ryuichi Watanabe, Catherine Roullier, Toshiyuki Suzuki, Philipp Hess, Christine Herrenknecht. Characterization of maitotoxin‐4 (MTX4) using electrospray positive mode ionization high‐resolution mass spectrometry and UV spectroscopy. Rapid Communications in Mass Spectrometry. 2020; 34 (19):e8859.
Chicago/Turabian StyleFrancesco Pisapia; Manoëlla Sibat; Ryuichi Watanabe; Catherine Roullier; Toshiyuki Suzuki; Philipp Hess; Christine Herrenknecht. 2020. "Characterization of maitotoxin‐4 (MTX4) using electrospray positive mode ionization high‐resolution mass spectrometry and UV spectroscopy." Rapid Communications in Mass Spectrometry 34, no. 19: e8859.
Coolia is a genus of marine benthic dinoflagellates which is widely distributed in tropical and temperate zones. Toxicity has been reported in selected Coolia species, although the identity of causative compounds is still controversial. In this study, we investigated the taxonomical and toxicological aspects of Coolia species from Brazil. Since light- and electron microscopy-based morphology was not enough to distinguish small-celled species, ITS and LSU D1-D3 phylogenetic analyses were used for species definition. Cultures of Coolia palmyrensis and Coolia santacroce were established from samples collected along the northeastern Brazilian coast, the first record of both species in South Atlantic waters. Cultures of Coolia malayensis and Coolia tropicalis were also established and exhibited acute in vivo toxicity to adults of Artemia salina, while C. palmyrensis and C. santacroce were non-toxic. The presence of 30 yessotoxin analogues, 7 metabolites of Coolia and 44 Gambierdiscus metabolites was screened in 14 strains of Coolia. 44-methyl gambierone (formerly referred to as MTX3) and a new isomer of this compound were detected only in C. tropicalis, using both low- and high-resolution LC-MS/MS. To our knowledge, this is the first report of gambierone analogues in dinoflagellates other than Gambierdiscus; the role of C. tropicalis in ciguatera poisoning thus deserves to be considered in further investigations.
Carlos Eduardo Junqueira De Azevedo Tibiriçá; Manoella Sibat; Luciano Felício Fernandes; Gwenaël Bilien; Nicolas Chomérat; Philipp Hess; Luiz L. Mafra Jr; Luiz L. Mafra. Diversity and Toxicity of the Genus Coolia Meunier in Brazil, and Detection of 44-methyl Gambierone in Coolia tropicalis. Toxins 2020, 12, 327 .
AMA StyleCarlos Eduardo Junqueira De Azevedo Tibiriçá, Manoella Sibat, Luciano Felício Fernandes, Gwenaël Bilien, Nicolas Chomérat, Philipp Hess, Luiz L. Mafra Jr, Luiz L. Mafra. Diversity and Toxicity of the Genus Coolia Meunier in Brazil, and Detection of 44-methyl Gambierone in Coolia tropicalis. Toxins. 2020; 12 (5):327.
Chicago/Turabian StyleCarlos Eduardo Junqueira De Azevedo Tibiriçá; Manoella Sibat; Luciano Felício Fernandes; Gwenaël Bilien; Nicolas Chomérat; Philipp Hess; Luiz L. Mafra Jr; Luiz L. Mafra. 2020. "Diversity and Toxicity of the Genus Coolia Meunier in Brazil, and Detection of 44-methyl Gambierone in Coolia tropicalis." Toxins 12, no. 5: 327.
Ciguatera poisoning is a foodborne illness caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates from the genera Gambierdiscus and Fukuyoa. The suitability of Solid Phase Adsorption Toxin Tracking (SPATT) technology for the monitoring of dissolved CTXs in the marine environment has recently been demonstrated. To refine the use of this passive monitoring tool in ciguateric areas, the effects of deployment time and sampler format on the adsorption of CTXs by HP20 resin were assessed in Anaho Bay (Nuku Hiva Island, French Polynesia), a well-known ciguatera hotspot. Toxicity data assessed by means of the mouse neuroblastoma cell-based assay (CBA-N2a) showed that a 24 h deployment of 2.5 g of resin allowed concentrating quantifiable amounts of CTXs on SPATT samplers. The CTX levels varied with increasing deployment time, resin load, and surface area. In addition to CTXs, okadaic acid (OA) and dinophysistoxin-1 (DTX1) were also detected in SPATT extracts using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS), consistent with the presence of Gambierdiscus and Prorocentrum species in the environment, as assessed by quantitative polymerase chain reaction (qPCR) and high-throughput sequencing (HTS) metabarcoding analyses conducted on passive window screen (WS) artificial substrate samples. Although these preliminary findings await further confirmation in follow-up studies, they highlight the usefulness of SPATT samplers in the routine surveillance of CP risk on a temporal scale, and the monitoring of other phycotoxin-related risks in ciguatera-prone areas.
Mélanie Roué; Kirsty F. Smith; Manoella Sibat; Jérôme Viallon; Kévin Henry; André Ung; Laura Biessy; Philipp Hess; Hélène Taiana Darius; Mireille Chinain. Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers. Toxins 2020, 12, 321 .
AMA StyleMélanie Roué, Kirsty F. Smith, Manoella Sibat, Jérôme Viallon, Kévin Henry, André Ung, Laura Biessy, Philipp Hess, Hélène Taiana Darius, Mireille Chinain. Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers. Toxins. 2020; 12 (5):321.
Chicago/Turabian StyleMélanie Roué; Kirsty F. Smith; Manoella Sibat; Jérôme Viallon; Kévin Henry; André Ung; Laura Biessy; Philipp Hess; Hélène Taiana Darius; Mireille Chinain. 2020. "Assessment of Ciguatera and Other Phycotoxin-Related Risks in Anaho Bay (Nuku Hiva Island, French Polynesia): Molecular, Toxicological, and Chemical Analyses of Passive Samplers." Toxins 12, no. 5: 321.
Over the last decade, knowledge has significantly increased on the taxonomic identity and distribution of dinoflagellates of the genera Gambierdiscus and Fukuyoa. Additionally, a number of hitherto unknown bioactive metabolites have been described, while the role of these compounds in ciguatera poisoning (CP) remains to be clarified. Ciguatoxins and maitotoxins are very toxic compounds produced by these dinoflagellates and have been described since the 1980s. Ciguatoxins are generally described as the main contributors to this food intoxication. Recent reports of CP in temperate waters of the Canary Islands (Spain) and the Madeira archipelago (Portugal) triggered the need for isolation and cultivation of dinoflagellates from these areas, and their taxonomic and toxicological characterization. Maitotoxins, and specifically maitotoxin-4, has been described as one of the most toxic compounds produced by these dinoflagellates (e.g., G. excentricus) in the Canary Islands. Thus, characterization of toxin profiles of Gambierdiscus species from adjacent regions appears critical. The combination of liquid chromatography coupled to either low- or high-resolution mass spectrometry allowed for characterization of several strains of Gambierdiscus and Fukuyoa from the Mediterranean Sea and the Canary Islands. Maitotoxin-3, two analogues tentatively identified as gambieric acid C and D, a putative gambierone analogue and a putative gambieroxide were detected in all G. australes strains from Menorca and Mallorca (Balearic Islands, Spain) while only maitotoxin-3 was present in an F. paulensis strain of the same region. An unidentified Gambierdiscus species (Gambierdiscus sp.2) from Crete (Greece) showed a different toxin profile, detecting both maitotoxin-3 and gambierone, while the availability of a G. excentricus strain from the Canary Islands (Spain) confirmed the presence of maitotoxin-4 in this species. Overall, this study shows that toxin profiles not only appear to be species-specific but probably also specific to larger geographic regions.
Pablo Estevez; Manoëlla Sibat; José Manuel Leão-Martins; Angels Tudó; Maria Rambla-Alegre; Katerina Aligizaki; Jorge Diogène; Ana Gago-Martinez; Philipp Hess. Use of Mass Spectrometry to Determine the Diversity of Toxins Produced by Gambierdiscus and Fukuyoa Species from Balearic Islands and Crete (Mediterranean Sea) and the Canary Islands (Northeast Atlantic). Toxins 2020, 12, 305 .
AMA StylePablo Estevez, Manoëlla Sibat, José Manuel Leão-Martins, Angels Tudó, Maria Rambla-Alegre, Katerina Aligizaki, Jorge Diogène, Ana Gago-Martinez, Philipp Hess. Use of Mass Spectrometry to Determine the Diversity of Toxins Produced by Gambierdiscus and Fukuyoa Species from Balearic Islands and Crete (Mediterranean Sea) and the Canary Islands (Northeast Atlantic). Toxins. 2020; 12 (5):305.
Chicago/Turabian StylePablo Estevez; Manoëlla Sibat; José Manuel Leão-Martins; Angels Tudó; Maria Rambla-Alegre; Katerina Aligizaki; Jorge Diogène; Ana Gago-Martinez; Philipp Hess. 2020. "Use of Mass Spectrometry to Determine the Diversity of Toxins Produced by Gambierdiscus and Fukuyoa Species from Balearic Islands and Crete (Mediterranean Sea) and the Canary Islands (Northeast Atlantic)." Toxins 12, no. 5: 305.
Ciguatera poisoning (CP) is a common seafood intoxication mainly caused by the consumption of fish contaminated by ciguatoxins. Recent studies showed that Caribbean ciguatoxin-1 (C-CTX1) is the main toxin causing CP through fish caught in the Northeast Atlantic, e.g., Canary Islands (Spain) and Madeira (Portugal). The use of liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) combined with neuroblastoma cell assay (N2a) allowed the initial confirmation of the presence of C-CTX1 in contaminated fish samples from the abovementioned areas, nevertheless the lack of commercially available reference materials for these particular ciguatoxin (CTX) analogues has been a major limitation to progress research. The EuroCigua project allowed the preparation of C-CTX1 laboratory reference material (LRM) from fish species (Seriola fasciata) from the Madeira archipelago (Portugal). This reference material was used to implement a liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) for the detection of C-CTX1, acquisition of full-scan as well as collision-induced mass spectra of this particular analogue. Fragmentation pathways were proposed based on fragments obtained. The optimized LC-HRMS method was then applied to confirm C-CTX1 in fish (Bodianus scrofa) caught in the Selvagens Islands (Portugal).
Pablo Estevez; Manoella Sibat; José Manuel Leão-Martins; Pedro Reis Costa; Ana Gago-Martínez; Philipp Hess. Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts. Toxins 2020, 12, 267 .
AMA StylePablo Estevez, Manoella Sibat, José Manuel Leão-Martins, Pedro Reis Costa, Ana Gago-Martínez, Philipp Hess. Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts. Toxins. 2020; 12 (4):267.
Chicago/Turabian StylePablo Estevez; Manoella Sibat; José Manuel Leão-Martins; Pedro Reis Costa; Ana Gago-Martínez; Philipp Hess. 2020. "Liquid Chromatography Coupled to High-Resolution Mass Spectrometry for the Confirmation of Caribbean Ciguatoxin-1 as the Main Toxin Responsible for Ciguatera Poisoning Caused by Fish from European Atlantic Coasts." Toxins 12, no. 4: 267.
Ciguatera poisoning (CP) is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. The toxin production and toxin profiles were explored in four clones of G. polynesiensis originating from different islands in French Polynesia with contrasted CP risk: RIK7 (Mangareva, Gambier), NHA4 (Nuku Hiva, Marquesas), RAI-1 (Raivavae, Australes), and RG92 (Rangiroa, Tuamotu). Productions of CTXs, maitotoxins (MTXs), and gambierone group analogs were examined at exponential and stationary growth phases using the neuroblastoma cell-based assay and liquid chromatography–tandem mass spectrometry. While none of the strains was found to produce known MTX compounds, all strains showed high overall P-CTX production ranging from 1.1 ± 0.1 to 4.6 ± 0.7 pg cell−1. In total, nine P-CTX analogs were detected, depending on strain and growth phase. The production of gambierone, as well as 44-methylgamberione, was also confirmed in G. polynesiensis. This study highlighted: (i) intraspecific variations in toxin production and profiles between clones from distinct geographic origins and (ii) the noticeable increase in toxin production of both CTXs, in particular CTX4A/B, and gambierone group analogs from the exponential to the stationary phase.
Sébastien Longo; Manoella Sibat; Jérôme Viallon; Hélène Taiana Darius; Philipp Hess; Mireille Chinain. Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia. Toxins 2019, 11, 735 .
AMA StyleSébastien Longo, Manoella Sibat, Jérôme Viallon, Hélène Taiana Darius, Philipp Hess, Mireille Chinain. Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia. Toxins. 2019; 11 (12):735.
Chicago/Turabian StyleSébastien Longo; Manoella Sibat; Jérôme Viallon; Hélène Taiana Darius; Philipp Hess; Mireille Chinain. 2019. "Intraspecific Variability in the Toxin Production and Toxin Profiles of In Vitro Cultures of Gambierdiscus polynesiensis (Dinophyceae) from French Polynesia." Toxins 11, no. 12: 735.
Dinoflagellates of the genus Dinophysis produce okadaic acid (OA) and analogues. Cultures of a strain of Dinophysis acuta isolated from the Galician Rías contained high levels of pectenotoxins but little OA. Isomeric forms of the latter were suspected as the concentration of OA increased after alkaline hydrolysis. High resolution mass spectra of candidate compounds suggest the presence of an OA-C9-diol ester, reported for the first time in Dinophysis acuta.
Manoella Sibat; María García-Portela; Philipp Hess. First identification of a C9-diol-ester of okadaic acid in Dinophysis acuta from Galician Rías Baixas (NW Spain). Toxicon 2018, 153, 19 -22.
AMA StyleManoella Sibat, María García-Portela, Philipp Hess. First identification of a C9-diol-ester of okadaic acid in Dinophysis acuta from Galician Rías Baixas (NW Spain). Toxicon. 2018; 153 ():19-22.
Chicago/Turabian StyleManoella Sibat; María García-Portela; Philipp Hess. 2018. "First identification of a C9-diol-ester of okadaic acid in Dinophysis acuta from Galician Rías Baixas (NW Spain)." Toxicon 153, no. : 19-22.
Ciguatera Fish Poisoning (CFP) is primarily caused by consumption of tropical and sub-tropical fish contaminated by Ciguatoxins (CTXs). These lipid-soluble, polyether neurotoxins are produced by dinoflagellates in the genera Gambierdiscus and Fukuyoa. While there is no regulatory level in Europe for CTXs, the European Food Safety Authority (EFSA) adopted the United States guidance level of 0.01 µg P-CTX1B eq. kg-1 of fish. This limit is extremely low and requires significant improvement in the detection of CTXs. In this study, we compared analytical protocols based on liquid chromatography coupled to tandem low or high resolution mass spectrometry (LC-LRMS or HRMS) to find the best conditions for sensitivity and/or selectivity. Different approaches such as LC conditions, ion choice and acquisition modes, were evaluated to detect the Pacific-ciguatoxins (P-CTXs) on a triple quadrupole (API4000 Qtrap, Sciex) or a quadrupole time of flight (QTOF 6550, Agilent Technologies) spectrometer. Moreover, matrix effects were calculated using matrix-matched calibration solutions of P-CTX1B and P-CTX3C prepared in purified fish extract. Subsequently, the method performance was assessed on naturally contaminated samples of seafood and phytoplankton. With LRMS, the ammoniated adduct ion used as a precursor ion showed an advantage for selectivity through confirmatory transitions, without affecting signal-to-noise ratios, and hence limits of detection (LODs). As also reported by some studies in the literature, methanol-based mobile phase gave better selectivity and sensitivity for the detection of P-CTXs. While the LOD for P-CTX1B and P-CTX3C met the EFSA recommendation level when using LRMS, the findings suggested careful evaluation of instrumental parameters for determination of CTXs. LODs were significantly higher for HRMS, which currently results in the need for a significantly higher sample intake. Nevertheless, HRMS allowed for the identification of artefacts and may allow for improved confirmation of the identity of P-CTXs analogues. Consequently, LRMS and HRMS are considered complementary to ensure adequate quantitation and identification of P-CTXs.
Manoella Sibat; Christine Herrenknecht; Hélène Taiana Darius; Mélanie Roué; Mireille Chinain; Philipp Hess. Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS). Journal of Chromatography A 2018, 1571, 16 -28.
AMA StyleManoella Sibat, Christine Herrenknecht, Hélène Taiana Darius, Mélanie Roué, Mireille Chinain, Philipp Hess. Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS). Journal of Chromatography A. 2018; 1571 ():16-28.
Chicago/Turabian StyleManoella Sibat; Christine Herrenknecht; Hélène Taiana Darius; Mélanie Roué; Mireille Chinain; Philipp Hess. 2018. "Detection of pacific ciguatoxins using liquid chromatography coupled to either low or high resolution mass spectrometry (LC-MS/MS)." Journal of Chromatography A 1571, no. : 16-28.
Ciguatoxins (CTXs) are potent algal toxins that cause widespread ciguatera poisoning and are found ubiquitously in coral reef food webs. Here we developed an environmentally-relevant, experimental model of CTX trophic transfer involving dietary exposure of herbivorous fish to the CTX-producing microalgae Gambierdiscus polynesiensis. Juvenile Naso brevirostris were fed a gel-food embedded with microalgae for 16 weeks (89 cells g−1 fish daily, 0.4 μg CTX3C equiv kg−1 fish). CTXs in muscle tissue were detectable after 2 weeks at levels above the threshold for human intoxication (1.2 ± 0.2 μg CTX3C equiv kg−1). Although tissue CTX concentrations stabilized after 8 weeks (∼3 ± 0.5 μg CTX3C equiv kg−1), muscle toxin burden (total μg CTX in muscle tissue) continued to increase linearly through the end of the experiment (16 weeks). Toxin accumulation was therefore continuous, yet masked by somatic growth dilution. The observed CTX concentrations, accumulation rates, and general absence of behavioural signs of intoxication are consistent with field observations and indicate that this method of dietary exposure may be used to develop predictive models of tissue-specific CTX uptake, metabolism and depuration. Results also imply that slow-growing fish may accumulate higher CTX flesh concentrations than fast-growing fish, which has important implications for global seafood safety.
Rachel J. Clausing; Barbara Losen; Francois R. Oberhaensli; Hélène Taiana Darius; Manoella Sibat-Dubois; Philipp Hess; Peter W. Swarzenski; Mireille Chinain; Marie-Yasmine Dechraoui Bottein. Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish. Aquatic Toxicology 2018, 200, 257 -265.
AMA StyleRachel J. Clausing, Barbara Losen, Francois R. Oberhaensli, Hélène Taiana Darius, Manoella Sibat-Dubois, Philipp Hess, Peter W. Swarzenski, Mireille Chinain, Marie-Yasmine Dechraoui Bottein. Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish. Aquatic Toxicology. 2018; 200 ():257-265.
Chicago/Turabian StyleRachel J. Clausing; Barbara Losen; Francois R. Oberhaensli; Hélène Taiana Darius; Manoella Sibat-Dubois; Philipp Hess; Peter W. Swarzenski; Mireille Chinain; Marie-Yasmine Dechraoui Bottein. 2018. "Experimental evidence of dietary ciguatoxin accumulation in an herbivorous coral reef fish." Aquatic Toxicology 200, no. : 257-265.
Ciguatera is a foodborne disease caused by the consumption of seafood contaminated with ciguatoxins (CTXs). Ciguatera-like poisoning events involving giant clams (Tridacna maxima) are reported occasionally from Pacific islands communities. The present study aimed at providing insights into CTXs tissue distribution and detoxification rate in giant clams exposed to toxic cells of Gambierdiscus polynesiensis, in the framework of seafood safety assessment. In a first experiment, three groups of tissue (viscera, flesh and mantle) were dissected from exposed individuals, and analyzed for their toxicity using the neuroblastoma cell-based assay (CBA-N2a) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. The viscera, flesh, and mantle were shown to retain 65%, 25%, and 10% of the total toxin burden, respectively. All tissues reached levels above the safety limit recommended for human consumption, suggesting that evisceration alone, a practice widely used among local populations, is not enough to ensure seafood safety. In a second experiment, the toxin content in contaminated giant clams was followed at different time points (0, 2, 4, and 6 days post-exposure). Observations suggest that no toxin elimination is visible in T. maxima throughout 6 days of detoxification.
Mélanie Roué; Hélène Taiana Darius; André Ung; Jérôme Viallon; Manoella Sibat; Philipp Hess; Zouher Amzil; Mireille Chinain. Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. Toxins 2018, 10, 189 .
AMA StyleMélanie Roué, Hélène Taiana Darius, André Ung, Jérôme Viallon, Manoella Sibat, Philipp Hess, Zouher Amzil, Mireille Chinain. Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis. Toxins. 2018; 10 (5):189.
Chicago/Turabian StyleMélanie Roué; Hélène Taiana Darius; André Ung; Jérôme Viallon; Manoella Sibat; Philipp Hess; Zouher Amzil; Mireille Chinain. 2018. "Tissue Distribution and Elimination of Ciguatoxins in Tridacna maxima (Tridacnidae, Bivalvia) Fed Gambierdiscus polynesiensis." Toxins 10, no. 5: 189.
Photosynthetic species of the genus Dinophysis are obligate mixotrophs with temporary plastids (kleptoplastids) that are acquired from the ciliate Mesodinium rubrum, which feeds on cryptophytes of the Teleaulax-Plagioselmis-Geminigera clade. A metabolomic study of the three-species food chain Dinophysis-Mesodinium-Teleaulax was carried out using mass spectrometric analysis of extracts of batch-cultured cells of each level of that food chain. The main goal was to compare the metabolomic expression of Galician strains of Dinophysis acuminata and D. acuta that were subjected to different feeding regimes (well-fed and prey-limited) and feeding on two Mesodinium (Spanish and Danish) strains. Both Dinophysis species were able to grow while feeding on both Mesodinium strains, although differences in growth rates were observed. Toxin and metabolomic profiles of the two Dinophysis species were significantly different, and also varied between different feeding regimes and different prey organisms. Furthermore, significantly different metabolomes were expressed by a strain of D. acuminata that was feeding on different strains of the ciliate Mesodinium rubrum. Both species-specific metabolites and those common to D. acuminata and D. acuta were tentatively identified by screening of METLIN and Marine Natural Products Dictionary databases. This first metabolomic study applied to Dinophysis acuminata and D.acuta in culture establishes a basis for the chemical inventory of these species.
María García-Portela; Beatriz Reguera; Manoella Sibat; Andreas Altenburger; Francisco Rodríguez; Philipp Hess. Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey. Marine Drugs 2018, 16, 143 .
AMA StyleMaría García-Portela, Beatriz Reguera, Manoella Sibat, Andreas Altenburger, Francisco Rodríguez, Philipp Hess. Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey. Marine Drugs. 2018; 16 (5):143.
Chicago/Turabian StyleMaría García-Portela; Beatriz Reguera; Manoella Sibat; Andreas Altenburger; Francisco Rodríguez; Philipp Hess. 2018. "Metabolomic Profiles of Dinophysis acuminata and Dinophysis acuta Using Non-Targeted High-Resolution Mass Spectrometry: Effect of Nutritional Status and Prey." Marine Drugs 16, no. 5: 143.
The sea urchin Tripneustes gratilla (Toxopneustidae, Echinoids) is a source of protein for many islanders in the Indo-West Pacific. It was previously reported to occasionally cause ciguatera-like poisoning; however, the exact nature of the causative agent was not confirmed. In April and July 2015, ciguatera poisonings were reported following the consumption of T. gratilla in Anaho Bay (Nuku Hiva Island, Marquesas archipelago, French Polynesia). Patient symptomatology was recorded and sea urchin samples were collected from Anaho Bay in July 2015 and November 2016. Toxicity analysis using the neuroblastoma cell–based assay (CBA-N2a) detected the presence of ciguatoxins (CTXs) in T. gratilla samples. Gambierdiscus species were predominant in the benthic assemblages of Anaho Bay, and G. polynesiensis was highly prevalent in in vitro cultures according to qPCR results. Liquid chromatography–tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major ciguatoxin congener in toxic sea urchin samples, followed by 51-OH-P-CTX-3C, P-CTX-3C, P-CTX-4A, and P-CTX-4B. Between July 2015 and November 2016, the toxin content in T. gratilla decreased, but was consistently above the safety limit allowed for human consumption. This study provides evidence of CTX bioaccumulation in T. gratilla as a cause of ciguatera-like poisoning associated with a documented symptomatology.
Hélène Taiana Darius; Mélanie Roué; Manoella Sibat; Jérôme Viallon; Clémence Mahana Iti Gatti; Mark W. Vandersea; Patricia A. Tester; R. Wayne Litaker; Zouher Amzil; Philipp Hess; Mireille Chinain. Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins. Marine Drugs 2018, 16, 122 .
AMA StyleHélène Taiana Darius, Mélanie Roué, Manoella Sibat, Jérôme Viallon, Clémence Mahana Iti Gatti, Mark W. Vandersea, Patricia A. Tester, R. Wayne Litaker, Zouher Amzil, Philipp Hess, Mireille Chinain. Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins. Marine Drugs. 2018; 16 (4):122.
Chicago/Turabian StyleHélène Taiana Darius; Mélanie Roué; Manoella Sibat; Jérôme Viallon; Clémence Mahana Iti Gatti; Mark W. Vandersea; Patricia A. Tester; R. Wayne Litaker; Zouher Amzil; Philipp Hess; Mireille Chinain. 2018. "Toxicological Investigations on the Sea Urchin Tripneustes gratilla (Toxopneustidae, Echinoid) from Anaho Bay (Nuku Hiva, French Polynesia): Evidence for the Presence of Pacific Ciguatoxins." Marine Drugs 16, no. 4: 122.
Ciguatera fish poisoning (CFP) is a foodborne disease caused by the consumption of seafood (fish and marine invertebrates) contaminated with ciguatoxins (CTXs) produced by dinoflagellates in the genus Gambierdiscus. The report of a CFP-like mass-poisoning outbreak following the consumption of Tectus niloticus (Tegulidae, Gastropod) from Anaho Bay on Nuku Hiva Island (Marquesas archipelago, French Polynesia) prompted field investigations to assess the presence of CTXs in T. niloticus. Samples were collected from Anaho Bay, 1, 6 and 28 months after this poisoning outbreak, as well as in Taiohae and Taipivai bays. Toxicity analysis using the neuroblastoma cell-based assay (CBA-N2a) detected the presence of CTXs only in Anaho Bay T. niloticus samples. This is consistent with qPCR results on window screen samples indicating the presence of Gambierdiscus communities dominated by the species G. polynesiensis in Anaho Bay. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses revealed that P-CTX-3B was the major congener, followed by P-CTX-3C, P-CTX-4A and P-CTX-4B in toxic samples. Between July 2014 and November 2016, toxin content in T. niloticus progressively decreased, but was consistently above the safety limit recommended for human consumption. This study confirms for the first time T. niloticus as a novel vector of CFP in French Polynesia.
Hélène Taiana Darius; Mélanie Roué; Manoella Sibat; Jérôme Viallon; Clémence Mahana Iti Gatti; Mark W. Vandersea; Patricia A. Tester; R. Wayne Litaker; Zouher Amzil; Philipp Hess; Mireille Chinain. Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia). Toxins 2017, 10, 2 .
AMA StyleHélène Taiana Darius, Mélanie Roué, Manoella Sibat, Jérôme Viallon, Clémence Mahana Iti Gatti, Mark W. Vandersea, Patricia A. Tester, R. Wayne Litaker, Zouher Amzil, Philipp Hess, Mireille Chinain. Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia). Toxins. 2017; 10 (1):2.
Chicago/Turabian StyleHélène Taiana Darius; Mélanie Roué; Manoella Sibat; Jérôme Viallon; Clémence Mahana Iti Gatti; Mark W. Vandersea; Patricia A. Tester; R. Wayne Litaker; Zouher Amzil; Philipp Hess; Mireille Chinain. 2017. "Tectus niloticus (Tegulidae, Gastropod) as a Novel Vector of Ciguatera Poisoning: Detection of Pacific Ciguatoxins in Toxic Samples from Nuku Hiva Island (French Polynesia)." Toxins 10, no. 1: 2.