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Editorial on the Research Topic Regulation of Fruit Ripening and Senescence Fruit ripening and senescence comprise complex and highly coordinated molecular and biochemical processes involving ripening-associated genes, transcription factors, enzymes, repressors, signaling molecules, and metabolic pathways in both climacteric and non-climacteric fruits (Cherian et al., 2014; Fuentes et al., 2019), which account for fruit quality on one hand and post-harvest losses on the other. Therefore, studying the molecular mechanisms of fruit ripening and senescence have profound commercial implications. As the fruit ripens or enters senescence, it becomes susceptible to fungal pathogens (Alkan and Fortes, 2015), while fruit-pathogen interactions could accelerate ripening and senescence, resulting in fruit deterioration. Hence, common strategies to slow down senescence and preserve fruit quality include both pre- and post-harvest management practices and technological tools. This Research Topic aimed to study and characterize the endogenous molecular and biochemical regulators (i.e., hormones, molecules, and genetic components) and their mechanisms of action to regulate ripening, senescence, and disease resistance in fruit. This collection includes 10 original research articles reporting new information on hormonal control of fruit ripening (Upadhyay et al.; Khaksar and Sirikantaramas; Fresno and Munné-Bosch), the effect of exogenous application of signal molecules in post-harvest fruit quality (Yu et al.; Yao et al.; García-Pastor et al.), genetics studies of the fruit cell wall and texture modification-related enzymes (Wen et al.; Nakano et al.), antioxidant-related proteomic changes during ripening (Song et al.), and the effect of mutations of key transcription factors on fruit quality traits (Adaskaveg et al.) (Figure 1). These studies included various fruit species such as tomato, peach, sweet cherry, strawberry, pomegranate, and durian. Moreover, two important reviews on the role of alternative oxidase (Hewitt and Dhingra) and sugar signaling (Durán-Soria et al.) during ripening were included in this Research Topic (Figure 1). Figure 1. Overview of the different aspects addressed by authors in the Research Topic “Regulation of Fruit Ripening and Senescence.” ABA, abscisic acid; ARF, auxin response factor; Eth, ethylene; GSNOR, S-nitrosoglutathione reductase; H2S, hydrogen sulfide; JA, jasmonic acid; NO, nitric oxide; SA, salicylic acid; sHSP, small heat-shock proteins; for other abbreviations, see the respective references. Upadhyay et al. investigated the regulation of two small heat shock proteins (sHSP) by ethylene and the inhibitor of ethylene receptor 1-methylcyclopropene (1-MCP) during tomato fruit ripening (SlHSP17.7A and SlHSP17.7B). This study showed that a transgenic tomato line silenced in one of the ACC synthase genes (SlACS2), whose fruit produced 50% less ethylene, had higher expression of both sHSP genes at the transition stages [breaker (BR) and BR+3 days] compared to control fruit. Moreover, the expression of SlHSP 17.7A and SlHSP 17.7B were significantly down and upregulated, respectively, in the tomato ripening mutants rin/rin, nor/nor, and Nr/Nr compared to the wild-type. Authors concluded that ethylene, directly or in combination with the transcription factor SlMADS-RIN, regulates the sHSP transcript expression. Khaksar and Sirikantaramas showed that the auxin response factor (ARF), DzARF2A, transactivates ethylene biosynthetic genes. Also, DzARF2A expression was higher in fast-ripening durian cultivars and increased in response to auxin treatment. Therefore, DzARF2A was suggested to play an important role in auxin-ethylene crosstalk to regulate the fruit ripening process in durian. Fresno and Munné-Bosch analyzed three hormones (ABA, JA, and SA) that play a role in fruit development and fruit-microbe interactions on sweet cherry exocarp and mesocarp. The fruit's exocarp had significantly higher concentrations of JA and SA than the mesocarp, while ABA content was similar in both tissues. Authors also reported that endophytic microbial colonization was poor but increased with fruit development, while epiphytic fungi, such as Alternaria spp., increased in the mesocarp when the exocarp was cracked. Thus, the absence of concentrated levels of JA and SA and high ABA levels could probably stimulate microbial colonization of mesocarp tissues. Yu et al. investigated the role of nitric oxide (NO)-induced resistance to Monilinia fructicola in peach. Exogenous NO enhanced disease resistance via inhibition of S-nitrosoglutathione reductase (GSNOR) expression and enzyme activity. Also, NO and GSNOR inhibitor (N6022) enhanced the expression of systemic-acquired resistance (SAR)-related genes contributing to disease resistance. In tomato, Yao et al. demonstrated that the addition of hydrogen sulfide (H2S) to ethylene treatment maintained high chlorophyll, anthocyanin, and starch content during storage, attenuating the gene expression of the beta-amylase (BAM3) and ethylene-responsive transcription factors. H2S affected pigments' metabolism and the transformation of macromolecular to small molecular metabolites. Altogether, H2S delayed the ripening and senescence of tomato fruits during storage. García-Pastor et al. found that foliar spray application of salicylates (SA, ASA, or MeSA) produced a higher concentration of phenolics, anthocyanin, and ascorbic acid at harvest and during storage of pomegranate. Remarkably, salicylate treatments increased crop yield, and red color in pomegranate arils. Cell wall-degrading enzymes play a key role in fruit ripening (Forlani et al., 2019). Wen et al. performed a genome-wide analysis of pectin methylesterase (PME) in tomato and identified 57 non-redundant PME genes. By analyzing gene expression, three new PME genes were suggested to play a role in fruit ripening, where PE1 and PE2 isoforms could be...
Carlos R. Figueroa; Cai-Zhong Jiang; Carolina A. Torres; Ana M. Fortes; Noam Alkan. Editorial: Regulation of Fruit Ripening and Senescence. Frontiers in Plant Science 2021, 12, 1 .
AMA StyleCarlos R. Figueroa, Cai-Zhong Jiang, Carolina A. Torres, Ana M. Fortes, Noam Alkan. Editorial: Regulation of Fruit Ripening and Senescence. Frontiers in Plant Science. 2021; 12 ():1.
Chicago/Turabian StyleCarlos R. Figueroa; Cai-Zhong Jiang; Carolina A. Torres; Ana M. Fortes; Noam Alkan. 2021. "Editorial: Regulation of Fruit Ripening and Senescence." Frontiers in Plant Science 12, no. : 1.
Soil salinity is one of the most limiting stresses for crop productivity and quality worldwide. In this sense, jasmonates (JAs) have emerged as phytohormones that play essential roles in mediating plant response to abiotic stresses, including salt stress. Here, we reviewed the mechanisms underlying the activation and response of the JA-biosynthesis and JA-signaling pathways under saline conditions in Arabidopsis and several crops. In this sense, molecular components of JA-signaling such as MYC2 transcription factor and JASMONATE ZIM-DOMAIN (JAZ) repressors are key players for the JA-associated response. Moreover, we review the antagonist and synergistic effects between JA and other hormones such as abscisic acid (ABA). From an applied point of view, several reports have shown that exogenous JA applications increase the antioxidant response in plants to alleviate salt stress. Finally, we discuss the latest advances in genomic techniques for the improvement of crop tolerance to salt stress with a focus on jasmonates.
Celia Delgado; Freddy Mora-Poblete; Sunny Ahmar; Jen-Tsung Chen; Carlos Figueroa. Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools. International Journal of Molecular Sciences 2021, 22, 3082 .
AMA StyleCelia Delgado, Freddy Mora-Poblete, Sunny Ahmar, Jen-Tsung Chen, Carlos Figueroa. Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools. International Journal of Molecular Sciences. 2021; 22 (6):3082.
Chicago/Turabian StyleCelia Delgado; Freddy Mora-Poblete; Sunny Ahmar; Jen-Tsung Chen; Carlos Figueroa. 2021. "Jasmonates and Plant Salt Stress: Molecular Players, Physiological Effects, and Improving Tolerance by Using Genome-Associated Tools." International Journal of Molecular Sciences 22, no. 6: 3082.
Strawberry fruits are rich in flavonoids like proanthocyanidins and anthocyanins. Their biosynthesis and accumulation are controlled by the MYB-bHLH-WD40 (MBW) transcriptional complex, which is mainly formed by basic helix-loop-helix (bHLH) and MYB transcription factors (TFs). In Arabidopsis thaliana both bHLH and MYB TFs are repressed by JASMONATE ZIM-DOMAIN (JAZ) proteins, the key repressors of the jasmonate-signaling pathway. The aim of this research was the characterization of the FaJAZ1/8.1/9/10 proteins and molecular targets of signaling components and anthocyanin biosynthesis-related TFs of Fragaria × ananassa by protein–protein interactions. For this, domain compositions were studied by multiple alignments and phylogenetic analyses, while interactions were analyzed by yeast two-hybrid (Y2H) assays. We detected high conservation of FaJAZ proteins and jasmonate-signaling components, as well as FabHLHs and FaMYB10 TFs. Moreover, we report the F. × ananassa YABBY1 (FaYAB1) TF, which is related to anthocyanin biosynthesis in Arabidopsis, showed high conservation of functional domains. We demonstrated that FaJAZ repressors interacted with F. × ananassa NOVEL INTERACTOR OF JAZ (FaNINJA), FaMYC2, and JASMONATE ASSOCIATED MYC2-LIKE (FaJAM) proteins. Besides, transcription factors of MBW-complex like FabHLH3, FabHLH33, and FaMYB10, together with FaYAB1, were molecular targets of FaJAZ repressors, exhibiting specificity or redundancy of interaction depending on particular FaJAZ protein. Overall, these results suggest that interactions of jasmonate-signaling components are fully conserved, and anthocyanin biosynthesis might be regulated by JAZ repressors in F. × ananassa.
Adrián Garrido-Bigotes; Marcela Torrejón; Roberto Solano; Carlos R. Figueroa. Interactions of JAZ Repressors with Anthocyanin Biosynthesis-Related Transcription Factors of Fragaria × ananassa. Agronomy 2020, 10, 1586 .
AMA StyleAdrián Garrido-Bigotes, Marcela Torrejón, Roberto Solano, Carlos R. Figueroa. Interactions of JAZ Repressors with Anthocyanin Biosynthesis-Related Transcription Factors of Fragaria × ananassa. Agronomy. 2020; 10 (10):1586.
Chicago/Turabian StyleAdrián Garrido-Bigotes; Marcela Torrejón; Roberto Solano; Carlos R. Figueroa. 2020. "Interactions of JAZ Repressors with Anthocyanin Biosynthesis-Related Transcription Factors of Fragaria × ananassa." Agronomy 10, no. 10: 1586.
Arrayan and peumo fruits are commonly used in the traditional medicine of Chile. In this study, the concentration of the extracts halving the bacterial viability and biofilms formation and disruption of the drug-sensitive and drug-resistant strains of Staphylococcus aureus and Pseudomonas aeruginosa was determined. The chemical composition of extracts was analyzed by high-resolution liquid chromatography coupled with mass spectrometry (U-HPLC/MS). The arrayan extract (Inhibitory concentration IC50 0.35 ± 0.01 mg/mL) was more effective than peumo extract (IC50 0.53 ± 0.02 mg/mL) in the inhibition of S. aureus planktonic cells. Similarly, the arrayan extract was more effective in inhibiting the adhesion (S. aureus IC50 0.23 ± 0.02 mg/mL, P. aeruginosa IC50 0.29 ± 0.02 mg/mL) than peumo extracts (S. aureus IC50 0.47 ± 0.03 mg/mL, P. aeruginosa IC50 0.35 ± 0.01 mg/mL). Both extracts inhibited quorum sensing in a concentration-dependent manner, and the most significant was the autoinducer-2 type communication inhibition by arrayan extract. Both extracts also disrupted preformed biofilm of P. aeruginosa (arrayan IC50 0.56 ± 0.04 mg/mL, peumo IC50 0.59 ± 0.04 mg/mL). However, neither arrayan nor peumo extracts disrupted S. aureus mature biofilm. U-HPLC/MS showed that both fruit extracts mainly possessed quercetin compounds; the peumo fruit extract also contained phenolic acids and phenylpropanoids. Our results suggested that both extracts could be used as natural antimicrobials for some skin and nosocomial infections.
Jitka Viktorová; Rohitesh Kumar; Kateřina Řehořová; Lan Hoang; Tomas Ruml; Carlos R. Figueroa; Monika Valdenegro; Lida Fuentes. Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba). Antibiotics 2020, 9, 444 .
AMA StyleJitka Viktorová, Rohitesh Kumar, Kateřina Řehořová, Lan Hoang, Tomas Ruml, Carlos R. Figueroa, Monika Valdenegro, Lida Fuentes. Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba). Antibiotics. 2020; 9 (8):444.
Chicago/Turabian StyleJitka Viktorová; Rohitesh Kumar; Kateřina Řehořová; Lan Hoang; Tomas Ruml; Carlos R. Figueroa; Monika Valdenegro; Lida Fuentes. 2020. "Antimicrobial Activity of Extracts of Two Native Fruits of Chile: Arrayan (Luma apiculata) and Peumo (Cryptocarya alba)." Antibiotics 9, no. 8: 444.
The phytohormone jasmonoyl-isoleucine (JA-Ile) regulates fundamental plant processes as developmental and defense responses. JA-Ile mediates the interaction between the F-box protein COI1 (part of the SCFCOI1 E3 ubiquitin ligase) and a JAZ repressor leading to early jasmonate responses. The Arabidopsis JAZ1 protein contains the canonical LPIARR degron sequence, which is responsible for the stabilization of the AtCOI1-JA-Ile-AtJAZ1 complex. In strawberry (Fragaria × ananassa) JAZ family was described at the transcriptional level during fruit development but the information about the interaction mode of this complex is still scarce at the molecular level. To gain insight into the strawberry JA-Ile receptor complex, we evaluated the interaction at the structural level, and protein models were built and analyzed for FaCOI1 and FaJAZ1, FaJAZ8.1, and FaJAZ10. The interaction between FaCOI1 and FaJAZ1, FaJAZ8.1 and FaJAZ10 were explored using several ligands, through molecular docking and molecular dynamics (MD) simulations, finding the strongest interaction with (+)-7-iso-JA-Ile than other ligands. Additionally, we tested interactions between FaCOI1 and FaJAZs by yeast two-hybrid assays in the presence of coronatine (COR, a JA-Ile mimic). We detected strong COR-dependent interactions between FaCOI1 and FaJAZ1. Interestingly, FaJAZ1 contains a new non-canonical (IPMQRK) functional degron sequence, in which Arg and Lys are the key residues for maintaining the interaction of the FaCOI1–COR–FaJAZ1 complex as we observed in mutated versions of the FaJAZ1 degron. Phylogenetic analysis showed that the IPMQRK degron is only present in orthologs belonging to the Rosoideae but not in other Rosaceae subfamilies. Together, this study uncovers a new degron sequence in plants, which could be required to make an alternative and functional JA-Ile perception complex in strawberry.
Adrián Garrido-Bigotes; Felipe Valenzuela-Riffo; Marcela Torrejón; Roberto Solano; Luis Morales-Quintana; Carlos R. Figueroa. A new functional JAZ degron sequence in strawberry JAZ1 revealed by structural and interaction studies on the COI1–JA-Ile/COR–JAZs complexes. Scientific Reports 2020, 10, 1 -17.
AMA StyleAdrián Garrido-Bigotes, Felipe Valenzuela-Riffo, Marcela Torrejón, Roberto Solano, Luis Morales-Quintana, Carlos R. Figueroa. A new functional JAZ degron sequence in strawberry JAZ1 revealed by structural and interaction studies on the COI1–JA-Ile/COR–JAZs complexes. Scientific Reports. 2020; 10 (1):1-17.
Chicago/Turabian StyleAdrián Garrido-Bigotes; Felipe Valenzuela-Riffo; Marcela Torrejón; Roberto Solano; Luis Morales-Quintana; Carlos R. Figueroa. 2020. "A new functional JAZ degron sequence in strawberry JAZ1 revealed by structural and interaction studies on the COI1–JA-Ile/COR–JAZs complexes." Scientific Reports 10, no. 1: 1-17.
Preharvest applications of methyl jasmonate (MeJA) have been shown to improve post-harvest fruit quality in strawberry fruit. However, the effectiveness of consecutive field applications at different phenological stages on the reinforcement of the antioxidant capacity remains to be analyzed. To determine the best antioxidant response of strawberry (Fragaria × ananassa ‘Camarosa’) fruit to different numbers and timing of MeJA applications, we performed three differential preharvest treatments (M1, M2, and M3) consisted of successive field applications of 250 μmol L–1 MeJA at flowering (M3), large green (M2 and M3), and ripe fruit stages (M1, M2, and M3). Then, we analyzed their effects on fruit quality parameters [firmness, skin color, soluble solids content/titratable acidity (SSC/TA) ratio, fruit weight at harvest, and weight loss] along with anthocyanin and proanthocyanidin (PA) accumulation; the antioxidant-related enzymatic activity of catalase (CAT), guaiacol peroxidase (POX), and ascorbate peroxidase (APX); the total flavonoid and phenolic contents, antioxidant capacity, and ascorbic acid content (AAC) during post-harvest storage (0, 24, 48, and 72 h). We also evaluated the effect on lignin, total carbon and nitrogen (%C and N), lipid peroxidation, and C and N isotopes signatures on fruits. Remarkably, the results indicated that MeJA treatment increases anthocyanin and PA contents as well as CAT activity in post-harvest storage, depending on the number of preharvest MeJA applications. Also, M3 fruit showed a higher AAC compared to control at 48 and 72 h. Noticeably, the anthocyanin content and CAT activity were more elevated in M3 treatment comparing with control at all post-harvest times. In turn, APX activity was found higher on all MeJA-treated fruit independent of the number of applications. Unlike, MeJA applications did not generate variations on fruit firmness and weight, lignin contents,% C and N, and in lipid peroxidation and water/nitrogen use efficiency according to C and N isotope discrimination. Finally, we concluded that an increasing number of MeJA applications (M3 treatment) improve anthocyanin, PA, AAC, and CAT activity that could play an essential role against reactive oxygen species, which cause stress that affects fruits during post-harvest storage.
Paz E. Zuñiga; Yasna Castañeda; Oscar Arrey; Lida Fuentes; Felipe Aburto; Carlos R. Figueroa. Methyl Jasmonate Applications From Flowering to Ripe Fruit Stages of Strawberry (Fragaria × ananassa ‘Camarosa’) Reinforce the Fruit Antioxidant Response at Post-harvest. Frontiers in Plant Science 2020, 11, 1 .
AMA StylePaz E. Zuñiga, Yasna Castañeda, Oscar Arrey, Lida Fuentes, Felipe Aburto, Carlos R. Figueroa. Methyl Jasmonate Applications From Flowering to Ripe Fruit Stages of Strawberry (Fragaria × ananassa ‘Camarosa’) Reinforce the Fruit Antioxidant Response at Post-harvest. Frontiers in Plant Science. 2020; 11 ():1.
Chicago/Turabian StylePaz E. Zuñiga; Yasna Castañeda; Oscar Arrey; Lida Fuentes; Felipe Aburto; Carlos R. Figueroa. 2020. "Methyl Jasmonate Applications From Flowering to Ripe Fruit Stages of Strawberry (Fragaria × ananassa ‘Camarosa’) Reinforce the Fruit Antioxidant Response at Post-harvest." Frontiers in Plant Science 11, no. : 1.
Several attempts have been made to study the effects of methyl jasmonate (MeJA) on plants in the past years. However, the comparative effects of the number and phenological time of MeJA applications on the activation of defense systems is currently unknown in strawberries. In the present research, we performed three field treatments during strawberry (Fragaria × ananassa ‘Camarosa’) fruit development and ripening which consisted of differential MeJA applications at flowering (M3), and the large green (M2 and M3) and red ripe (M1, M2, and M3) fruit stages. We also checked changes in gene expression related to plant defense against Botrytis cinerea inoculation post-harvest. In M3 treatment, we observed an upregulation of the anthocyanin and lignin contents and the defense-related genes, encoding for chitinases, β-1,3-glucanases and polygalacturonase-inhibiting proteins, after harvest (0 hpi), along with the jasmonate signaling-related genes FaMYC2 and FaJAZ1 at 48 h after B. cinerea inoculation (48 hpi) during postharvest storage. Although we did not find differences in gray mold incidence between the MeJA treatments and control, these results suggest that preharvest MeJA treatment from the flowering stage onwards (M3) primes defense responses mediated by the upregulation of different defense-related genes and retains the upregulation of MYC2 and JAZ1 at 48 hpi.
Felipe Valenzuela-Riffo; Paz E. Zúñiga; Luis Morales-Quintana; Mauricio Lolas; Marcela Cáceres; Carlos R. Figueroa. Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits. Plants 2020, 9, 447 .
AMA StyleFelipe Valenzuela-Riffo, Paz E. Zúñiga, Luis Morales-Quintana, Mauricio Lolas, Marcela Cáceres, Carlos R. Figueroa. Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits. Plants. 2020; 9 (4):447.
Chicago/Turabian StyleFelipe Valenzuela-Riffo; Paz E. Zúñiga; Luis Morales-Quintana; Mauricio Lolas; Marcela Cáceres; Carlos R. Figueroa. 2020. "Priming of Defense Systems and Upregulation of MYC2 and JAZ1 Genes after Botrytis cinerea Inoculation in Methyl Jasmonate-Treated Strawberry Fruits." Plants 9, no. 4: 447.
Jasmonates are phytohormones that regulate development, metabolism and immunity. Signal transduction is critical to activate jasmonate responses, but the evolution of some key regulators such as jasmonate-ZIM domain (JAZ) repressors is not clear. Here, we identified 1065 JAZ sequence proteins in 66 lower and higher plants and analyzed their evolution by bioinformatics methods. We found that the TIFY and Jas domains are highly conserved along the evolutionary scale. Furthermore, the canonical degron sequence LPIAR(R/K) of the Jas domain is conserved in lower and higher plants. It is noteworthy that degron sequences showed a large number of alternatives from gymnosperms to dicots. In addition, ethylene-responsive element binding factor-associated amphiphilic repression (EAR) motifs are displayed in all plant lineages from liverworts to angiosperms. However, the cryptic MYC2-interacting domain (CMID) domain appeared in angiosperms for the first time. The phylogenetic analysis performed using the Maximum Likelihood method indicated that JAZ ortholog proteins are grouped according to their similarity and plant lineage. Moreover, ancestral JAZ sequences were constructed by PhyloBot software and showed specific changes in the TIFY and Jas domains during evolution from liverworts to dicots. Finally, we propose a model for the evolution of the ancestral sequences of the main eight JAZ protein subgroups. These findings contribute to the understanding of the JAZ family origin and expansion in land plants.
Adrián Garrido-Bigotes; Felipe Valenzuela-Riffo; Carlos R. Figueroa. Evolutionary Analysis of JAZ Proteins in Plants: An Approach in Search of the Ancestral Sequence. International Journal of Molecular Sciences 2019, 20, 5060 .
AMA StyleAdrián Garrido-Bigotes, Felipe Valenzuela-Riffo, Carlos R. Figueroa. Evolutionary Analysis of JAZ Proteins in Plants: An Approach in Search of the Ancestral Sequence. International Journal of Molecular Sciences. 2019; 20 (20):5060.
Chicago/Turabian StyleAdrián Garrido-Bigotes; Felipe Valenzuela-Riffo; Carlos R. Figueroa. 2019. "Evolutionary Analysis of JAZ Proteins in Plants: An Approach in Search of the Ancestral Sequence." International Journal of Molecular Sciences 20, no. 20: 5060.
Fragaria chiloensis is an herbaceous perennial undomesticated species with a high potential for sustainable fruit production in areas where drought and salinity represent a constraint on crop growth and productivity. The present study aimed to examine the genetic variability of fruit weight, firmness and color parameters (L*, a*, b*, Hue angle and Chroma) in wild-collected accessions from south-central Chile. A Bayesian analysis of genetic parameters showed that the traits under study were found to be moderately to highly heritable with estimates from the marginal posterior distributions ranging between 0.26 (firmness) and 0.67 (L*). Interestingly, Hue and Chroma had relatively high values of broad-sense heritability, with the Bayesian credible set: H2 = 0.53–0.76 and H2 = 0.42–0.66, respectively. A Bayesian bi-trait analysis showed a positive genetic correlation (r^g = 0.65, probability of the posterior samples <0.05) between fruit weight and Hue, which agreed with the existence of two pleiotropic loci [from simple sequence repeats (SSR) markers FvH4045 and CFACT084 on linkage groups FG3A and FG2A/2B/2C] with probabilities of the posterior estimates of 0.022 and <0.001, respectively. According to the Bayesian credible region, 5 (of 12) correlation estimates were significantly different from zero. Hue and Chroma showed a high and negative genetic correlation (r^g = −0.762), but no significant pleotropic locus. The results are promising and suggest that the genetic variation found among wild populations of F. chiloensis supports the idea of domesticating this crop for sustainable fruit production systems.
Freddy Mora; Paz E. Zúñiga; Carlos R. Figueroa. Genetic Variation and Trait Correlations for Fruit Weight, Firmness and Color Parameters in Wild Accessions of Fragaria chiloensis. Agronomy 2019, 9, 506 .
AMA StyleFreddy Mora, Paz E. Zúñiga, Carlos R. Figueroa. Genetic Variation and Trait Correlations for Fruit Weight, Firmness and Color Parameters in Wild Accessions of Fragaria chiloensis. Agronomy. 2019; 9 (9):506.
Chicago/Turabian StyleFreddy Mora; Paz E. Zúñiga; Carlos R. Figueroa. 2019. "Genetic Variation and Trait Correlations for Fruit Weight, Firmness and Color Parameters in Wild Accessions of Fragaria chiloensis." Agronomy 9, no. 9: 506.
In recent years, there has been an increasing interest in studying food and its derived ingredients that can provide beneficial effects for human health. These studies are helping to understand the bases of the ancestral use of several natural products, including native fruits as functional foods. As a result, the polyphenol profile and the antioxidant capacity of the extracts obtained from different Patagonian native berries have been described. This review aims to provide valuable information regarding fruit quality, its particular compound profile, and the feasibility of producing functional foods for human consumption to prevent disorders such as metabolic syndrome and cardiovascular diseases. We also discuss attempts concerning the domestication of these species and generating knowledge that strengthens their potential as traditional fruits in the food market and as a natural heritage for future generations. Finally, additional efforts are still necessary to fully understand the potential beneficial effects of the consumption of these berries on human health, the application of suitable technology for postharvest improvement, and the generation of successfully processed foods derived from Patagonian berries.
Lida Fuentes; Carlos R. Figueroa; Monika Valdenegro; Raúl Vinet. Patagonian Berries: Healthy Potential and the Path to Becoming Functional Foods. Foods 2019, 8, 289 .
AMA StyleLida Fuentes, Carlos R. Figueroa, Monika Valdenegro, Raúl Vinet. Patagonian Berries: Healthy Potential and the Path to Becoming Functional Foods. Foods. 2019; 8 (8):289.
Chicago/Turabian StyleLida Fuentes; Carlos R. Figueroa; Monika Valdenegro; Raúl Vinet. 2019. "Patagonian Berries: Healthy Potential and the Path to Becoming Functional Foods." Foods 8, no. 8: 289.
Fleshy fruits are characterized by having a developmentally and genetically controlled, highly intricate ripening process, leading to dramatic modifications in fruit size, texture, color, flavor, and aroma. Climacteric fruits such as tomato, pear, banana, and melon show a ripening-associated increase in respiration and ethylene production and these processes are well-documented. In contrast, the hormonal mechanism of fruit development and ripening in non-climacteric fruit, such as strawberry, grape, raspberry, and citrus, is not well characterized. However, recent studies have shown that non-climacteric fruit development and ripening, involves the coordinated action of different hormones, such as abscisic acid (ABA), auxin, gibberellins, ethylene, and others. In this review, we discuss and evaluate the recent research findings concerning the hormonal regulation of non-climacteric fruit development and ripening and their cross-talk by taking grape, strawberry, and raspberry as reference fruit species.
Lida Fuentes; Carlos R. Figueroa; Monika Valdenegro. Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening. Horticulturae 2019, 5, 45 .
AMA StyleLida Fuentes, Carlos R. Figueroa, Monika Valdenegro. Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening. Horticulturae. 2019; 5 (2):45.
Chicago/Turabian StyleLida Fuentes; Carlos R. Figueroa; Monika Valdenegro. 2019. "Recent Advances in Hormonal Regulation and Cross-Talk during Non-Climacteric Fruit Development and Ripening." Horticulturae 5, no. 2: 45.
The phytohormone jasmonoyl-isoleucine (JA-Ile) regulates fundamental plant processes. Fragaria vesca, the woodland strawberry, is a model plant for the Rosaceae family, in which the JA-Ile perception is poorly understood at the molecular level. JA-Ile promotes binding of JAZ repressor to COI1 protein in Arabidopsis to activate jasmonate (JA)-dependent responses. The aim of this work was to understand the molecular basis of the interaction between the F. vesca COI1 (FvCOI1) and JAZ1 (FvJAZ1) promoted by JA-Ile using a computational approach. Multiple sequence alignments and phylogenetic analyses of amino acid sequences were performed for FvCOI1, FvJAZ1 and their ortholog sequences. 3D structures for FvCOI1 and FvJAZ1 proteins were built by methods of homology modeling, using AtCOI1-JA-Ile-AtJAZ1 as template and then they were further refined and validated by molecular dynamics (MD) simulation. A molecular docking approach along with MDS analysis were used to gain insights into the interaction between a putative degron-like sequence present in FvJAZ1 with the FvCOI1-JA-Ile complex. FvCOI1 and FvJAZ1 showed high and moderate sequence identity, respectively, with the corresponding ortholog proteins from other plant species including apple, grape, tomato and Arabidopsis. Moreover, the FvJAZ1 has a variant C-terminal IPMQRK sequence instead of the canonical LPIARR degron sequence located in the Jas domain of AtJAZ1. The MD simulation results showed that the FvCOI1-JA-Ile-FvJAZ1 complex was stable, and the IPMQRK peptide of FvJAZ1 directly interacted with FvCOI1 and JA-Ile. The present research provides novel insight into the molecular interactions among key JA-signaling components in the model plant F. vesca, being few examples of characterized JA-Ile receptors at a structural level in plants.
Felipe Valenzuela-Riffo; Adrián Garrido-Bigotes; Pablo M. Figueroa; Luis Morales-Quintana; Carlos R. Figueroa. Structural analysis of the woodland strawberry COI1-JAZ1 co-receptor for the plant hormone jasmonoyl-isoleucine. Journal of Molecular Graphics and Modelling 2018, 85, 250 -261.
AMA StyleFelipe Valenzuela-Riffo, Adrián Garrido-Bigotes, Pablo M. Figueroa, Luis Morales-Quintana, Carlos R. Figueroa. Structural analysis of the woodland strawberry COI1-JAZ1 co-receptor for the plant hormone jasmonoyl-isoleucine. Journal of Molecular Graphics and Modelling. 2018; 85 ():250-261.
Chicago/Turabian StyleFelipe Valenzuela-Riffo; Adrián Garrido-Bigotes; Pablo M. Figueroa; Luis Morales-Quintana; Carlos R. Figueroa. 2018. "Structural analysis of the woodland strawberry COI1-JAZ1 co-receptor for the plant hormone jasmonoyl-isoleucine." Journal of Molecular Graphics and Modelling 85, no. : 250-261.
Fleshy fruits are an important source of anthocyanins and proanthocyanidins (PAs), which protect plants against stress, and their consumption provides beneficial effects for human health. In strawberry fruit, the application of exogenous methyl jasmonate (MeJA) upregulates anthocyanin accumulation, although the relationship between the jasmonate pathway and anthocyanin and PA biosynthesis in fruits remains to be understood. Anthocyanin and PA accumulation is mainly regulated at the transcriptional level through R2R3-MYB and bHLH transcription factors in different plant species and organs. Here, the effect of jarin-1, a specific inhibitor of bioactive JA (jasmonoyl-isoleucine, JA-Ile) biosynthesis, on anthocyanin and PA accumulation was evaluated during strawberry (Fragaria × ananassa) fruit development using an in vitro ripening system for 48 h. Also, we observed the effects of MeJA and the application of jarin-1 to MeJA-treated fruits (MeJA + jarin-1 treatment). We assessed changes of expression levels for the JA-Ile and MeJA biosynthetic (FaJAR1.2 and FaJMT), JA signaling-related (FaMYC2 and FaJAZ1), MYB-bHLH-WD40 (MBW) complex-related (FabHLH3/33, FaMYB9/10/11, and repressor FaMYB1), and anthocyanin and PA biosynthetic (FaANS, FaUFGT, FaANR, and FaLAR) genes. In addition, the promoter region of MBW complex-related MYB genes was isolated and sequenced. We found a higher redness of strawberry fruit skin and anthocyanin content in MeJA-treated fruits with respect to jarin-1-treated ones concomitant with an upregulation of FaANS and FaUFGT genes. Inversely, the PA content was higher in jarin-1- and MeJA + jarin-1-treated than in MeJA-treated fruits. MeJA + jarin-1 treatment resulted in an upregulation of FaANR and associated transcription factors such as FabHLH33 and FaMYB9/11 along with FaJMT and FaJAR1.2. Finally, we found JA-responsive elements in the promoter regions of FaMYB1/9/10/11 genes. It is proposed that PA biosynthesis-related genes can be upregulated by the application of jarin-1 to MeJA-treated fruit, thus increasing PA accumulation in strawberry.
Laura D. Delgado; Paz E. Zuñiga; Nicolás E. Figueroa; Edgar Pastene; Hugo F. Escobar-Sepúlveda; Pablo M. Figueroa; Adrián Garrido-Bigotes; Carlos R. Figueroa. Application of a JA-Ile Biosynthesis Inhibitor to Methyl Jasmonate-Treated Strawberry Fruit Induces Upregulation of Specific MBW Complex-Related Genes and Accumulation of Proanthocyanidins. Molecules 2018, 23, 1433 .
AMA StyleLaura D. Delgado, Paz E. Zuñiga, Nicolás E. Figueroa, Edgar Pastene, Hugo F. Escobar-Sepúlveda, Pablo M. Figueroa, Adrián Garrido-Bigotes, Carlos R. Figueroa. Application of a JA-Ile Biosynthesis Inhibitor to Methyl Jasmonate-Treated Strawberry Fruit Induces Upregulation of Specific MBW Complex-Related Genes and Accumulation of Proanthocyanidins. Molecules. 2018; 23 (6):1433.
Chicago/Turabian StyleLaura D. Delgado; Paz E. Zuñiga; Nicolás E. Figueroa; Edgar Pastene; Hugo F. Escobar-Sepúlveda; Pablo M. Figueroa; Adrián Garrido-Bigotes; Carlos R. Figueroa. 2018. "Application of a JA-Ile Biosynthesis Inhibitor to Methyl Jasmonate-Treated Strawberry Fruit Induces Upregulation of Specific MBW Complex-Related Genes and Accumulation of Proanthocyanidins." Molecules 23, no. 6: 1433.
Jasmonates (JAs) are signalling molecules involved in stress responses, development and secondary metabolism biosynthesis, although their roles in fleshy-fruit development and ripening processes are not well known. In strawberry fruit, it has been proposed that JAs could regulate the early development through the activation of the JAs biosynthesis. Moreover, it has been reported that JA treatment increases anthocyanin content in strawberry fruit involving the bioactive jasmonate biosynthesis. Nevertheless, JA signalling pathway, of which main components are the COI1-JAZ co-receptor and the MYC transcription factors (TFs), has not been characterized in strawberry until now. Here we identified and characterized the woodland strawberry (Fragaria vesca) JAZ and MYC genes as well as studied their expression during development and ripening stages in commercial strawberry (Fragaria × ananassa) fruit. We described twelve putative JAZ proteins and two MYC TFs, which showed high conservation with respect to their orthologs in Arabidopsis thaliana and in other fleshy-fruit species such as Malus × domestica, Vitis vinifera and Solanum lycopersicum as revealed by gene synteny and phylogenetic analyses. Noteworthy, their expression levels exhibited a significant decrease from fruit development to ripening stages in F. × ananassa, along with others of the JA signalling-related genes such as FaNINJA and FaJAMs, encoding for negative regulators of JA responses. Moreover, we found that main JA signalling-related genes such as FaMYC2, and FaJAZ1 are promptly induced by JA treatment at early times in F. × ananassa fruit. These results suggest the conservation of the canonical JA signalling pathway in strawberry and a possible role of this pathway in early strawberry fruit development, which also correlates negatively with the beginning of the ripening process.
Adrián Garrido-Bigotes; Nicolás E. Figueroa; Pablo M. Figueroa; Carlos R. Figueroa. Jasmonate signalling pathway in strawberry: Genome-wide identification, molecular characterization and expression of JAZs and MYCs during fruit development and ripening. PLOS ONE 2018, 13, e0197118 .
AMA StyleAdrián Garrido-Bigotes, Nicolás E. Figueroa, Pablo M. Figueroa, Carlos R. Figueroa. Jasmonate signalling pathway in strawberry: Genome-wide identification, molecular characterization and expression of JAZs and MYCs during fruit development and ripening. PLOS ONE. 2018; 13 (5):e0197118.
Chicago/Turabian StyleAdrián Garrido-Bigotes; Nicolás E. Figueroa; Pablo M. Figueroa; Carlos R. Figueroa. 2018. "Jasmonate signalling pathway in strawberry: Genome-wide identification, molecular characterization and expression of JAZs and MYCs during fruit development and ripening." PLOS ONE 13, no. 5: e0197118.
The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and 342 bp. Lower genetic diversity at the species level (HE = 0.17, Shannon’s index = 0.28) was found compared to previous studies of this species. No climatic region pattern for SSR diversity was observed. Structure analysis suggests that the accessions are grouped into three significantly differentiated clusters. Pairwise estimates of φST indicated a low degree of differentiation between the three genetic groups (φST = 0.023 to 0.06). These groups are in concordance with potential glacial refugia in the region, with many accessions being an admixture of them.
Felipe Alberto Oñate; Rodrigo Hasbun; Freddy Mora; Carlos Rodrigo Figueroa. Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry. Acta Scientiarum. Agronomy 2018, 40, 34966 .
AMA StyleFelipe Alberto Oñate, Rodrigo Hasbun, Freddy Mora, Carlos Rodrigo Figueroa. Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry. Acta Scientiarum. Agronomy. 2018; 40 (1):34966.
Chicago/Turabian StyleFelipe Alberto Oñate; Rodrigo Hasbun; Freddy Mora; Carlos Rodrigo Figueroa. 2018. "Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry." Acta Scientiarum. Agronomy 40, no. 1: 34966.
Background. The phytohormone jasmonates (JAs) regulate fundamental plant processes; such as the anthocyanin accumulation during ripening of strawberry, a non-climacteric fruit model. Jasmonoyl-isoleucine (JA-Ile), one of the bioactive JA molecules, mediates binding of the JAZ repressor protein to COI1, an F-box protein forming the SCFCOI1 ubiquitin E3 ligase complex, in Arabidopsis. The COI1-JA-Ile-JAZ complex initiates the JA-signaling pathway leading to early jasmonate responses. Most of Arabidopsis JAZs contain a degron sequence at the Jas domain responsible for interaction with COI1 and JA-Ile. The woodland strawberry (Fragaria vesca) is a model plant for the Rosaceae family, in which the JA-signaling pathway is poorly understood at the molecular level. The aim of this work was to understand the molecular basis of the interaction between the F. vesca COI1 (FvCOI1) and JAZ1 (FvJAZ1) or JAZ8 (FvJAZ8) mediated by JA-Ile. Methods. Multiple alignments of amino acid sequences and phylogenetic analyses were performed for FvCOI1 and FvJAZ1/8 and their ortholog sequences. The FvCOI1 and FvJAZ1/8 3D structures were built by homology modeling methods, which were further refined and validated by molecular dynamics simulation (MDS). A molecular docking approach along with MDS analysis were used to understand the interaction capacity between a putative degron-like present in FvJAZ1 and FvJAZ8 with the FvCOI1-JA-Ile and FvCOI1-JA complexes. Results. FvCOI1 and FvJAZ1/8 showed high and moderate identity, respectively, with the corresponding ortholog proteins from other plant species including apple, grape, tomato and Arabidopsis. The resulting FvCOI1 structural model showed that the F-box and LRR domains were highly similar to that described in Arabidopsis COI1 (AtCOI1) crystal structure. Unexpectedly, we found that FvJAZ1 has a variant IPMQRK sequence respect to the canonical LPIAR(R/K) degron sequence observed in AtJAZ1. The MDS results showed that the FvCOI1-JA-Ile-FvJAZ1 complex was the most stable among all the analyzed ones, and the IPMQRK peptide of FvJAZ1 interacted directly with FvCOI1 and JA-Ile. In contrast, FvJAZ8 did not show a direct interaction with those two components, as expected from previous experimental results for the ortholog AtJAZ8. Discussion. The present research provides novel insight into the molecular interactions between key JA-signaling components in the model plant F. vesca. Remarkably, we characterized the IPMQRK sequence present in FvJAZ1, a putative variant of the canonical degron previously described in AtJAZ1. We propose that the FvCOI1-JA-Ile-FvJAZ1 complex is stable, and that the degron-like sequence present in FvJAZ1 interacts in a steady manner with FvCOI1-JA-Ile. Up to now, this is the first structural characterization of molecular interactions that may be occurring between the core components of the JA-Ile perception complex in a fleshy fruit-related species.
Felipe Valenzuela-Riffo; Adrián Garrido-Bigotes; Pablo M Figueroa; Luis Morales-Quintana; Carlos R Figueroa. Structural characterization of the jasmonoyl-isoleucine perception complexes from Fragaria vesca by in silico molecular analysis. 2018, 1 .
AMA StyleFelipe Valenzuela-Riffo, Adrián Garrido-Bigotes, Pablo M Figueroa, Luis Morales-Quintana, Carlos R Figueroa. Structural characterization of the jasmonoyl-isoleucine perception complexes from Fragaria vesca by in silico molecular analysis. . 2018; ():1.
Chicago/Turabian StyleFelipe Valenzuela-Riffo; Adrián Garrido-Bigotes; Pablo M Figueroa; Luis Morales-Quintana; Carlos R Figueroa. 2018. "Structural characterization of the jasmonoyl-isoleucine perception complexes from Fragaria vesca by in silico molecular analysis." , no. : 1.
Víctor H. Campos-Requena; Bernabé L. Rivas; Mónica A. Pérez; Carlos R. Figueroa; Nicolás E. Figueroa; Eugenio A. Sanfuentes. Corrigendum to “Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries – In vivo antimicrobial synergy over Botrytis cinerea” [Postharvest Biol. Technol. 129 (2017) 29–36]. Postharvest Biology and Technology 2017, 133, 122 .
AMA StyleVíctor H. Campos-Requena, Bernabé L. Rivas, Mónica A. Pérez, Carlos R. Figueroa, Nicolás E. Figueroa, Eugenio A. Sanfuentes. Corrigendum to “Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries – In vivo antimicrobial synergy over Botrytis cinerea” [Postharvest Biol. Technol. 129 (2017) 29–36]. Postharvest Biology and Technology. 2017; 133 ():122.
Chicago/Turabian StyleVíctor H. Campos-Requena; Bernabé L. Rivas; Mónica A. Pérez; Carlos R. Figueroa; Nicolás E. Figueroa; Eugenio A. Sanfuentes. 2017. "Corrigendum to “Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries – In vivo antimicrobial synergy over Botrytis cinerea” [Postharvest Biol. Technol. 129 (2017) 29–36]." Postharvest Biology and Technology 133, no. : 122.
Adrián Garrido‑Bigotes; Pablo M. Figueroa. Correction to: Jasmonate Metabolism and Its Relationship with Abscisic Acid During Strawberry Fruit Development and Ripening. Journal of Plant Growth Regulation 2017, 37, 705 -706.
AMA StyleAdrián Garrido‑Bigotes, Pablo M. Figueroa. Correction to: Jasmonate Metabolism and Its Relationship with Abscisic Acid During Strawberry Fruit Development and Ripening. Journal of Plant Growth Regulation. 2017; 37 (2):705-706.
Chicago/Turabian StyleAdrián Garrido‑Bigotes; Pablo M. Figueroa. 2017. "Correction to: Jasmonate Metabolism and Its Relationship with Abscisic Acid During Strawberry Fruit Development and Ripening." Journal of Plant Growth Regulation 37, no. 2: 705-706.
The Chilean strawberry (Fragaria chiloensis) fruit has interesting organoleptic properties, but its postharvest life is affected by gray mold decay caused by Botrytis cinerea. The effect of preharvest applications of methyl jasmonate (MeJA) or chitosan on the molecular defense-related responses and protection against gray mold decay were investigated in Chilean strawberry fruit during postharvest storage. Specifically, we inoculated harvested fruit with B. cinerea spores and studied the expression of genes encoding for the pathogenesis-related (PR) proteins β-1,3-glucanases (FcBG2-1, FcBG2-2 and FcBG2-3) and chitinases (FcCHI2-2 and FcCHI3-1), and for polygalacturonase inhibiting proteins (FcPGIP1 and FcPGIP2) at 0, 2, 24, 48, and 72 h post inoculation (hpi). Remarkably, MeJA- and chitosan-treated fruit exhibited a lower incidence of B. cinerea infection than the control-treated at 48 and 72 hpi. At the molecular level, both are efficient elicitors for priming in F. chiloensis fruit since we observed an upregulation of the FcBG2-1, FcBG2-3, FcPGIP1, and FcPGIP2 at 0 hpi. Moreover, a chitosan-mediated upregulation of FcPGIPs at early times post inoculation (2–24 hpi) and MeJA upregulated FcBGs (24–72 hpi) and FcPGIP1 at later times could contribute to reduce B. cinerea incidence by differential upregulation of defense genes. We concluded that preharvest applications of MeJA or chitosan had a long-lasting effect on the reduction of B. cinerea incidence during postharvest as well as an enhancer effect on the induction of PR and PGIP gene expression.
Gabriela M. Saavedra; Eugenio Sanfuentes; Pablo M. Figueroa; Carlos R. Figueroa. Independent Preharvest Applications of Methyl Jasmonate and Chitosan Elicit Differential Upregulation of Defense-Related Genes with Reduced Incidence of Gray Mold Decay during Postharvest Storage of Fragaria chiloensis Fruit. International Journal of Molecular Sciences 2017, 18, 1420 .
AMA StyleGabriela M. Saavedra, Eugenio Sanfuentes, Pablo M. Figueroa, Carlos R. Figueroa. Independent Preharvest Applications of Methyl Jasmonate and Chitosan Elicit Differential Upregulation of Defense-Related Genes with Reduced Incidence of Gray Mold Decay during Postharvest Storage of Fragaria chiloensis Fruit. International Journal of Molecular Sciences. 2017; 18 (7):1420.
Chicago/Turabian StyleGabriela M. Saavedra; Eugenio Sanfuentes; Pablo M. Figueroa; Carlos R. Figueroa. 2017. "Independent Preharvest Applications of Methyl Jasmonate and Chitosan Elicit Differential Upregulation of Defense-Related Genes with Reduced Incidence of Gray Mold Decay during Postharvest Storage of Fragaria chiloensis Fruit." International Journal of Molecular Sciences 18, no. 7: 1420.
Víctor H. Campos-Requena; Bernabé L. Rivas; Mónica A. Pérez; Carlos Figueroa; Nicolás E. Figueroa; Eugenio A. Sanfuentes. Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries − In vivo antimicrobial synergy over Botrytis cinerea. Postharvest Biology and Technology 2017, 129, 29 -36.
AMA StyleVíctor H. Campos-Requena, Bernabé L. Rivas, Mónica A. Pérez, Carlos Figueroa, Nicolás E. Figueroa, Eugenio A. Sanfuentes. Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries − In vivo antimicrobial synergy over Botrytis cinerea. Postharvest Biology and Technology. 2017; 129 ():29-36.
Chicago/Turabian StyleVíctor H. Campos-Requena; Bernabé L. Rivas; Mónica A. Pérez; Carlos Figueroa; Nicolás E. Figueroa; Eugenio A. Sanfuentes. 2017. "Thermoplastic starch/clay nanocomposites loaded with essential oil constituents as packaging for strawberries − In vivo antimicrobial synergy over Botrytis cinerea." Postharvest Biology and Technology 129, no. : 29-36.