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Associate Professor and Head of the Experimental Sciences Department, at the European University Miguel de Cervantes (Valladolid, Spain) from 2006 to present. Head of the Experimental Sciences Department Predoctoral, doctoral and postdoctoral research at the Department of Biochemistry and Molecular Biology, Faculty of Science, University of Valladolid (Valladolid, Spain). In addition to a postdoctoral training of more than 3 years in the Department of Biotechnology, NIAR Center, (Tsukuba, Japan). The lines of research carried out are wide but related to the effect of proteins, released by microorganisms, and synthesized by plants, on human health. Among them we highlight: Cloning of protein genes, biochemical analysis of protein inhibitors, isolation of proteins and inhibitors by chromatography, affinity study of glycoproteins and carbohydrates by ELISA techniques, determination of organic compounds by immunoaffinity, protein synthesis, protein synthesis inhibitors of chemical and protein origin (study and isolation), biochemical characterization of proteins, comparison of dental age study, salivary proteins, environmental microbiological analysis and dental biofilms.
It is widely recognized that periodontal disease is an inflammatory entity of infectious origin, in which the immune activation of the host leads to the destruction of the supporting tissues of the tooth. Periodontal pathogenic bacteria like Porphyromonas gingivalis, that belongs to the complex net of oral microflora, exhibits a toxicogenic potential by releasing endotoxins, which are the lipopolysaccharide component (LPS) available in the outer cell wall of Gram-negative bacteria. Endotoxins are released into the tissues causing damage after the cell is lysed. There are three well-defined regions in the LPS: one of them, the lipid A, has a lipidic nature, and the other two, the Core and the O-antigen, have a glycosidic nature, all of them with independent and synergistic functions. Lipid A is the “bioactive center” of LPS, responsible for its toxicity, and shows great variability along bacteria. In general, endotoxins have specific receptors at the cells, causing a wide immunoinflammatory response by inducing the release of pro-inflammatory cytokines and the production of matrix metalloproteinases. This response is not coordinated, favoring the dissemination of LPS through blood vessels, as well as binding mainly to Toll-like receptor 4 (TLR4) expressed in the host cells, leading to the destruction of the tissues and the detrimental effect in some systemic pathologies. Lipid A can also act as a TLRs antagonist eliciting immune deregulation. Although bacterial endotoxins have been extensively studied clinically and in a laboratory, their effects on the oral cavity and particularly on periodontium deserve special attention since they affect the connective tissue that supports the tooth, and can be linked to advanced medical conditions. This review addresses the distribution of endotoxins associated with periodontal pathogenic bacteria and its relationship with systemic diseases, as well as the effect of some therapeutic alternatives.
Rosalia Marcano; M. Rojo; Damián Cordoba-Diaz; Manuel Garrosa. Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease. Toxins 2021, 13, 533 .
AMA StyleRosalia Marcano, M. Rojo, Damián Cordoba-Diaz, Manuel Garrosa. Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease. Toxins. 2021; 13 (8):533.
Chicago/Turabian StyleRosalia Marcano; M. Rojo; Damián Cordoba-Diaz; Manuel Garrosa. 2021. "Pathological and Therapeutic Approach to Endotoxin-Secreting Bacteria Involved in Periodontal Disease." Toxins 13, no. 8: 533.
The safety of concentrated food complements intake is a major health concern. It has been well established that green tea polyphenols (GTPs) consumption promotes healthy effects. However, the ingestion of large amounts of GTPs is a matter of controversy due to reported adverse effects. We underwent a preliminary exploration of the effects of the oral administration of a standardized concentrated GTPs preparation on mice which suffered from reversible intestinal derangement promoted by sublethal amounts of the antiribosomal lectin ebulin f from dwarf elder (Sambucus ebulus L.). Neither independent oral administration of 30 mg/kg body weight Polyphenon 60 nor intraperitoneal administration of 2.5 mg/kg body weight ebulin f triggered lethal toxicity. In contrast, the simultaneous administration of these same doses of both Polyphenon 60 and ebulin f triggered an important and unexpected synergistic toxic action featured by the biphasic reduction of weight, which continued after eight days, reaching a reduction of 40%. Lethality appeared 2 days after the onset of the combined treatment and reached more than 50% after 10 days.
M. Ángeles Rojo; Manuel Garrosa; Pilar Jiménez; Tomás Girbés; Verónica Garcia-Recio; Manuel Cordoba-Diaz; Damián Cordoba-Diaz. Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin. Toxins 2020, 12, 542 .
AMA StyleM. Ángeles Rojo, Manuel Garrosa, Pilar Jiménez, Tomás Girbés, Verónica Garcia-Recio, Manuel Cordoba-Diaz, Damián Cordoba-Diaz. Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin. Toxins. 2020; 12 (9):542.
Chicago/Turabian StyleM. Ángeles Rojo; Manuel Garrosa; Pilar Jiménez; Tomás Girbés; Verónica Garcia-Recio; Manuel Cordoba-Diaz; Damián Cordoba-Diaz. 2020. "Unexpected Toxicity of Green Tea Polyphenols in Combination with the Sambucus RIL Ebulin." Toxins 12, no. 9: 542.
: Lactose is a reducing sugar consisting of galactose and glucose, linked by a β (1→4) glycosidic bond, considered as an antioxidant due to its α-hydroxycarbonyl group. Lactose is widely ingested through the milk and other unfermented dairy products and is considered to be one of the primary foods. On the other hand, lactose is also considered as one of the most widely used excipients for the development of pharmaceutical formulations. In this sense, lactose has been related to numerous drug-excipient or drug-food pharmacokinetic interactions. : Intolerance, maldigestion and malabsorption of carbohydrates are common disorders in clinical practice, with lactose-intolerance being the most frequently diagnosed, afflicting 10% of the world’s population. Four clinical subtypes of lactose intolerance may be distinguished, namely lactase deficiency in premature infants, congenital lactase deficiency, adult-type hypolactasia and secondary lactase intolerance. An overview of the main uses of lactose in human nutrition and in the pharmaceutical industry and the problems derived from this circumstance are described in this review.
Rafael G. Seoane; Verónica Garcia-Recio; Manuel Garrosa; María Á. Rojo; Pilar Jiménez; Tomás Girbés; Manuel Cordoba-Diaz; Damián Cordoba-Diaz. Human Health Effects of Lactose Consumption as a Food and Drug Ingredient. Current Pharmaceutical Design 2020, 26, 1778 -1789.
AMA StyleRafael G. Seoane, Verónica Garcia-Recio, Manuel Garrosa, María Á. Rojo, Pilar Jiménez, Tomás Girbés, Manuel Cordoba-Diaz, Damián Cordoba-Diaz. Human Health Effects of Lactose Consumption as a Food and Drug Ingredient. Current Pharmaceutical Design. 2020; 26 (16):1778-1789.
Chicago/Turabian StyleRafael G. Seoane; Verónica Garcia-Recio; Manuel Garrosa; María Á. Rojo; Pilar Jiménez; Tomás Girbés; Manuel Cordoba-Diaz; Damián Cordoba-Diaz. 2020. "Human Health Effects of Lactose Consumption as a Food and Drug Ingredient." Current Pharmaceutical Design 26, no. 16: 1778-1789.
Essential oils have great potential in the field of the food industry as they can effectively prevent the presence of several bacterial and fungal pathogens. Essential oils are complex volatile compounds, synthesized naturally in different plant parts during the process of the secondary metabolism. The main goal of this work is to perform a qualitative evaluation of the antibacterial properties of 24 chemotyped essential oils against the growth of Bacillus subtillis. These Gram-positive bacteria are responsible for “rope” disease in bread preservation processes. The study was carried out using the method of disk-diffusion in agar. Biological activity was observed in five essential oils: Cymbopogon martinii var. motia, Thymus vulgaris QT Linanol, Thymus satureioides, Mentha piperita and Eugenia caryophyllus. The first three have in common the presence of some mono terpenic derivatives—Geraniol, Linalool and Carvacrol, respectively—with strong antimicrobial effects. The Cymbopogon martinii essential oil is one of the botanicals with the highest geraniol content (up to 80.53%) and showed more activity antimicrobial than the others. A contributing role of this knowledge could be the design of Cymbopogon martinii essential oil formula, which can be used in bakery industry as a preservative, such as nano-encapsulation for bakery doughs, active packaging of baked products or surface disinfectants.
Sara Santamarta; A. Cristina Aldavero; M. Ángeles Rojo. Antibacterial Properties of Cymbopogon martinii Essential Oil against Bacillus subtillis Food Industry Pathogen. Proceedings 2020, 66, 1 .
AMA StyleSara Santamarta, A. Cristina Aldavero, M. Ángeles Rojo. Antibacterial Properties of Cymbopogon martinii Essential Oil against Bacillus subtillis Food Industry Pathogen. Proceedings. 2020; 66 (1):1.
Chicago/Turabian StyleSara Santamarta; A. Cristina Aldavero; M. Ángeles Rojo. 2020. "Antibacterial Properties of Cymbopogon martinii Essential Oil against Bacillus subtillis Food Industry Pathogen." Proceedings 66, no. 1: 1.
Investigating the spatial distribution of avian blood parasites can shed light on the occurrence of host switching and expansion in new territories, two key factors for which to account when addressing future parasite impacts on vertebrates. We examined the mitochondrial cytochrome b lineages of haemosporidians infecting the white-throated dipper Cinclus cinclus in order to assess their distribution across five mountains in the Central Iberian Peninsula and the possible implications of lineage transmission in that geographical context. Of 71 host individuals, 79.6% were infected with Haemoproteus spp., 0.9% with Plasmodium spp. and 19.5% with Leucocytozoon spp. We identified seven lineages from genus Haemoproteus spp. (four were novel), one lineage of Plasmodium spp. and nine of Leucocytozoon spp. (five previously undescribed). Only two of the Haemoproteus lineages (RW1 and MW1) showed widespread distribution across the study sites whereas the novel lineages each corresponded to a single area. Given the non-migratory behaviour of the host species in the region, our results provide the first proof of LeucocytozoonWW6 lineage transmission within Europe. Furthermore, this study is the first to reveal the transmission in Europe of Haemoproteus payevskyi and Haemoproteus nucleocondensus, corresponding to the identified RW1 and GRW01 lineages respectively. Both findings support the idea that these lineages could be transmitted year-round transcontinentally. Estudios sobre la distribución espacial de parásitos sanguíneos en aves pueden ayudar a aclarar la coincidencia de cambio de huésped y de la expansión en nuevos territorios de los hemospori- dios, dos factores clave a tener en cuenta al abordar los impactos futuros de los parásitos en los vertebrados. Para ello, examinamos los linajes mitocondriales citocromo b de hemosporidios que infectan al mirlo acuático Cinclus cinclus con el fin de evaluar su distribución en cinco áreas montañosas del centro de la península Ibérica y las posibles implicaciones de la transmisión del linaje en esas áreas. Se analizaron 71 aves, de las que el 79,6% estaban infectadas con Haemoproteus spp., 0,9% con Plasmodium spp. y 19,5% con Leucocytozoon spp. Se identificaron siete linajes de género Haemoproteus spp. (cuatro eran nuevos), un linaje de Plasmodium spp. y nueve de Leucocytozoon spp. (cinco no han sido descritos previamente). Sólo dos de los linajes de Haemoproteus spp. (RW1 y MW1) mostraron una amplia distribución en el área de estudio, mientras que los nuevos linajes fueron asignados a un área cada uno. Dado el comportamiento no migratorio del mirlo acuático en el área de estudio, nuestros resultados muestran la transmisión del linaje LeucocytozoonWW6 en Europa. Además, este estudio es el primero en revelar la transmisión en Europa de Haemoproteus payevskyi y Haemoproteus nucleocondensus, correspondiente a los linajes identificados RW1 y GRW01, respectivamente. Ambos hallazgos apoyan la idea de que estos linajes podrían ser transmitidos durante todo el año transcontinentalmente.
M. Ángeles Rojo; Maria Angeles Hernandez; Francisco Campos; Tomás Santamaría; Susana Dias; Patricia Casanueva. The Iberian Peninsula is an Area of Infection byHaemoproteus payevskyiandHaemoproteus nucleocondensusfor the White-Throated DipperCinclus cinclus. Ardeola 2015, 62, 373 -382.
AMA StyleM. Ángeles Rojo, Maria Angeles Hernandez, Francisco Campos, Tomás Santamaría, Susana Dias, Patricia Casanueva. The Iberian Peninsula is an Area of Infection byHaemoproteus payevskyiandHaemoproteus nucleocondensusfor the White-Throated DipperCinclus cinclus. Ardeola. 2015; 62 (2):373-382.
Chicago/Turabian StyleM. Ángeles Rojo; Maria Angeles Hernandez; Francisco Campos; Tomás Santamaría; Susana Dias; Patricia Casanueva. 2015. "The Iberian Peninsula is an Area of Infection byHaemoproteus payevskyiandHaemoproteus nucleocondensusfor the White-Throated DipperCinclus cinclus." Ardeola 62, no. 2: 373-382.
Maria Angeles Rojo; Francisco Campos; Tomás Santamaría; M. Angeles Hernández. Haemosporidians in Iberian BluethroatsLuscinia svecica. Ardeola 2014, 61, 135 -143.
AMA StyleMaria Angeles Rojo, Francisco Campos, Tomás Santamaría, M. Angeles Hernández. Haemosporidians in Iberian BluethroatsLuscinia svecica. Ardeola. 2014; 61 (1):135-143.
Chicago/Turabian StyleMaria Angeles Rojo; Francisco Campos; Tomás Santamaría; M. Angeles Hernández. 2014. "Haemosporidians in Iberian BluethroatsLuscinia svecica." Ardeola 61, no. 1: 135-143.
Capsule White-throated Dippers from southern Europe were found to be infected by three haemosporidians. Aims To examine the occurrence of blood parasites in dippers in the Iberian Peninsula and to investigate the relationship between prevalence levels, environmental factors and bird fitness. Methods White-throated Dippers were trapped with mist-nets from five montane areas in the centre of the Iberian Peninsula. Parasites were detected from blood samples by polymerase chain reaction screening. Results About half (51.3%) of 152 dippers showed some kind of infection. The genus Haemoproteus was recorded in 49.3% of the birds, and the genus Leucocytozoon in 19.1%, while Plasmodium was present only in 0.7%. Among the infected birds, 34.6% carried a double infection (Haemoproteus + Leucocytozoon). Prevalence did not differ between gender or age-classes, but it varied between study sites, being significantly higher in mountains with higher precipitation. There was a reduction in body mass owing to double infection in yearling males only. Conclusion This is the first work assessing parasite prevalence in dippers from southern Europe. Infection of dippers by haemosporidians is likely to be related to a study site's climatic envelope.
Maria Angeles Rojo; Francisco Campos; Maria Angeles Hernandez; Susana Dias; Elsa Santos; Tomás Santamaría; Luis Corrales. Prevalence of haematozoan parasites in the White-throated Dipper Cinclus cinclus in southern Europe. Bird Study 2013, 60, 247 -256.
AMA StyleMaria Angeles Rojo, Francisco Campos, Maria Angeles Hernandez, Susana Dias, Elsa Santos, Tomás Santamaría, Luis Corrales. Prevalence of haematozoan parasites in the White-throated Dipper Cinclus cinclus in southern Europe. Bird Study. 2013; 60 (2):247-256.
Chicago/Turabian StyleMaria Angeles Rojo; Francisco Campos; Maria Angeles Hernandez; Susana Dias; Elsa Santos; Tomás Santamaría; Luis Corrales. 2013. "Prevalence of haematozoan parasites in the White-throated Dipper Cinclus cinclus in southern Europe." Bird Study 60, no. 2: 247-256.
Raquel Munoz; Yolanda Arias; Jose Miguel Ferreras; Pilar Jimenez; Maria Angeles Rojo; Carmelo Bernabeu; Damian Cordoba-Diaz; Tomas Girbes. Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin. Medicinal Chemistry 2012, 8, 996 -1002.
AMA StyleRaquel Munoz, Yolanda Arias, Jose Miguel Ferreras, Pilar Jimenez, Maria Angeles Rojo, Carmelo Bernabeu, Damian Cordoba-Diaz, Tomas Girbes. Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin. Medicinal Chemistry. 2012; 8 (6):996-1002.
Chicago/Turabian StyleRaquel Munoz; Yolanda Arias; Jose Miguel Ferreras; Pilar Jimenez; Maria Angeles Rojo; Carmelo Bernabeu; Damian Cordoba-Diaz; Tomas Girbes. 2012. "Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin." Medicinal Chemistry 8, no. 6: 996-1002.
TGF-beta superfamily co-receptors are emerging as targets for cancer therapy, acting both directly on cells and indirectly on the tumour neovasculature. Endoglin (CD105), an accessory component of the TGF-beta receptor complex, is expressed in certain melanoma cell lines and the endothelial cells of tumour neovessels. Targeting endoglin with immunotoxins is an attractive approach for actively suppressing the blood supply to tumours. Here, we report evidence indicating that endoglin is expressed in mouse melanoma B16MEL4A5 and mouse fibroblast L929 cell lines. We prepared an immunotoxin to target endoglin by coupling the rat anti-mouse MJ7/18 (IgG2a) monoclonal antibody (mAb) to the non-toxic type 2 ribosome-inactivating protein nigrin b (Ngb) with N-succinimidyl 3-(2-pyridyldithio)-propionate (SPDP) as a linker with a molar nigrin b at a MJ7/18 stoichiometry of 2:1. The MJ7-Ngb immunotoxin generated killed both cell lines, with IC50 values of 4.2 × 10−9 M for B16MEL4A5 and 7.7 × 10−11 M for L929 cells. For in vivo assays of the immunotoxin, B16MEL4A5 cells were injected subcutaneously into the right flanks of 6-week-old C57BL/6 J mice. When the animals developed palpable solid tumours, they were subjected to treatment with the immunotoxin. While treatment with either MJ7/18 mAb or Ngb did not affect tumour development, treatment with the immunotoxin completely and steadily blocked tumour growth up to 7 days, after which some tumours re-grew. Thus, vascular-targeting therapy with this anti-vascular immunotoxin could promote the destruction of newly created tumour vessels at early stages of B16MEL4A5 tumour development and readily accessible CD105+ B16MEL4A5 melanoma cells.
Raquel Muñoz; Yolanda Arias; José Miguel Ferreras; Pilar Jiménez; Carmen Langa; Maria Angeles Rojo; Manuel José Gayoso; Damian Cordoba-Diaz; Carmelo Bernabeu; Tomás Girbes. In vitro and in vivo effects of an anti-mouse endoglin (CD105)–immunotoxin on the early stages of mouse B16MEL4A5 melanoma tumours. Cancer Immunology, Immunotherapy 2012, 62, 541 -551.
AMA StyleRaquel Muñoz, Yolanda Arias, José Miguel Ferreras, Pilar Jiménez, Carmen Langa, Maria Angeles Rojo, Manuel José Gayoso, Damian Cordoba-Diaz, Carmelo Bernabeu, Tomás Girbes. In vitro and in vivo effects of an anti-mouse endoglin (CD105)–immunotoxin on the early stages of mouse B16MEL4A5 melanoma tumours. Cancer Immunology, Immunotherapy. 2012; 62 (3):541-551.
Chicago/Turabian StyleRaquel Muñoz; Yolanda Arias; José Miguel Ferreras; Pilar Jiménez; Carmen Langa; Maria Angeles Rojo; Manuel José Gayoso; Damian Cordoba-Diaz; Carmelo Bernabeu; Tomás Girbes. 2012. "In vitro and in vivo effects of an anti-mouse endoglin (CD105)–immunotoxin on the early stages of mouse B16MEL4A5 melanoma tumours." Cancer Immunology, Immunotherapy 62, no. 3: 541-551.
Endoglin (CD105), a cell-surface co-receptor for transforming growth factor-beta (TGF-) superfamily members, is over-expressed in tumor neovasculature and can be targeted with anti-endoglin antibodies, thus becoming an important tool for anti-tumoral therapy. Injury of the mouse tail induced the transient expression of endoglin, this peaking at three days after injury and disappearing six days later. An immunotoxin containing the anti-mouse endoglin rat monoclonal antibody MJ7/18 and the non-toxic ribosome-inactivating protein nigrin b (Ngb) was found to be very active in targeting mouse endoglin in the L929 fibroblast cell line (IC50 of 4 x 10-11 M). At that concentration, the immunotoxin lacked unspecific activity. Upon induction of endoglin after injury, the MJ7-Ngb immunotoxin strongly attacked and deranged the injured tail, inducing tissue damage. Such effects were dependent on the age of the animals and were evident in six-week-old mice, but not in eight-month-old mice. Our results indicate that endoglin is up-regulated in newly formed vessels upon injury and can be targeted by the MJ7-Ngb immunotoxin; thus, it could be a useful tool for tumor ablation research.
Raquel Muñoz; Yolanda Arias; Jose Miguel Ferreras; Pilar Jimenez; Maria Angeles Rojo; Carmelo Bernabeu; Damian Cordoba-Diaz; Tomas Girbes. Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin. Medicinal Chemistry 2012, 8, 996 -1002.
AMA StyleRaquel Muñoz, Yolanda Arias, Jose Miguel Ferreras, Pilar Jimenez, Maria Angeles Rojo, Carmelo Bernabeu, Damian Cordoba-Diaz, Tomas Girbes. Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin. Medicinal Chemistry. 2012; 8 (6):996-1002.
Chicago/Turabian StyleRaquel Muñoz; Yolanda Arias; Jose Miguel Ferreras; Pilar Jimenez; Maria Angeles Rojo; Carmelo Bernabeu; Damian Cordoba-Diaz; Tomas Girbes. 2012. "Transient Injury-Dependent Up-Regulation of CD105 and its Specific Targeting with an Anti-Vascular Anti-Mouse Endoglin-Nigrin b Immunotoxin." Medicinal Chemistry 8, no. 6: 996-1002.
M. Angeles Hernández; Francisco Campos; Tomás Santamaría; Luis Corrales; Maria Angeles Rojo; Susana Dias. Genetic Differences Among Iberian White-Throated Dipper Cinclus cinclus Populations Based on the Cytochrome b Sequence. Ardeola 2012, 59, 111 -122.
AMA StyleM. Angeles Hernández, Francisco Campos, Tomás Santamaría, Luis Corrales, Maria Angeles Rojo, Susana Dias. Genetic Differences Among Iberian White-Throated Dipper Cinclus cinclus Populations Based on the Cytochrome b Sequence. Ardeola. 2012; 59 (1):111-122.
Chicago/Turabian StyleM. Angeles Hernández; Francisco Campos; Tomás Santamaría; Luis Corrales; Maria Angeles Rojo; Susana Dias. 2012. "Genetic Differences Among Iberian White-Throated Dipper Cinclus cinclus Populations Based on the Cytochrome b Sequence." Ardeola 59, no. 1: 111-122.
Apicomplexan blood parasites (genera Haemoproteus, Plasmodium and Leucocytozoon) prevalence in two related species (Reed Warbler Acrocephalus scirpaceus and Sedge Warbler A. schoenobaenus) was studied in 2006 at the Natural Reserve of Castronuño-Vega del Duero, Western Spain, a stopover area during the autumn migration. A fragment of the mitochondrial cytochrome b gene of the parasites was amplified, using a nested PCR assay, from avian blood samples. High prevalence of malaria parasites was found in both species, 84.6% in Reed Warbler and 71.8% in Sedge Warbler, and the degree of infection reach 100% of the population that breed at the Reserve, suggesting good conditions for the development of dipteran vectors in this area. By sequencing 464 nucleotides of the obtained fragments, we found four different mitochondrial haplotypes of Haemoproteus or Plasmodium in the two species analysed. Leucocytozoon infection was not detected, in contrast to the high prevalence of this parasite in other avian species in Spain, probably because the water course studied is not an adequate habitat for its vectors.
Mónica Fernández; Maria Angeles Rojo; Patricia Casanueva; Silvia Carrión; Maria Angeles Hernandez; Francisco Campos. High prevalence of haemosporidians in Reed Warbler Acrocephalus scirpaceus and Sedge Warbler Acrocephalus schoenobaenus in Spain. Journal of Ornithology 2009, 151, 27 -32.
AMA StyleMónica Fernández, Maria Angeles Rojo, Patricia Casanueva, Silvia Carrión, Maria Angeles Hernandez, Francisco Campos. High prevalence of haemosporidians in Reed Warbler Acrocephalus scirpaceus and Sedge Warbler Acrocephalus schoenobaenus in Spain. Journal of Ornithology. 2009; 151 (1):27-32.
Chicago/Turabian StyleMónica Fernández; Maria Angeles Rojo; Patricia Casanueva; Silvia Carrión; Maria Angeles Hernandez; Francisco Campos. 2009. "High prevalence of haemosporidians in Reed Warbler Acrocephalus scirpaceus and Sedge Warbler Acrocephalus schoenobaenus in Spain." Journal of Ornithology 151, no. 1: 27-32.
Young shoots of Sambucus ebulus L. contain a monomeric d-galactose binding lectin (SELlm), which disappears upon shoot development, and was previously undetected since it co-purifies with the non-toxic type 2 ribosome-inactivating protein ebulin l and the dimeric lectin SELld. Molecular cloning of cDNA coding for SELlm and mass spectrometry analysis revealed a protein with a molecular mass of 34,239 Da, which displays 80%, 77% and 45% of amino acid sequence identity with the ebulin l-B chain, SELld and ricin-B chain, respectively. Furthermore, the cloned precursor, with respect to the ebulin l precursor is truncated and contains the signal peptide, a piece of the A chain, a piece of the connecting peptide and the B chain. Further processing yields the lectin protein, which contains only the B chain. Despite the fact that SELlm displays the same d-galactose-binding sites than ricin, it was found that the lectin has different binding properties to d-galactose-containing matrix than ricin. Notably, and unlike ricin, the binding of SELlm and other Sambucus lectins to such matrix was maximum in range of 0–10 °C and abolished at 20 °C.
Lucía Citores; Maria Angeles Rojo; Pilar Jiménez; José M. Ferreras; Rosario Iglesias; Isabel Aranguez; Tomás Girbés. Transient occurrence of an ebulin-related d-galactose-lectin in shoots of Sambucus ebulus L. Phytochemistry 2008, 69, 857 -864.
AMA StyleLucía Citores, Maria Angeles Rojo, Pilar Jiménez, José M. Ferreras, Rosario Iglesias, Isabel Aranguez, Tomás Girbés. Transient occurrence of an ebulin-related d-galactose-lectin in shoots of Sambucus ebulus L. Phytochemistry. 2008; 69 (4):857-864.
Chicago/Turabian StyleLucía Citores; Maria Angeles Rojo; Pilar Jiménez; José M. Ferreras; Rosario Iglesias; Isabel Aranguez; Tomás Girbés. 2008. "Transient occurrence of an ebulin-related d-galactose-lectin in shoots of Sambucus ebulus L." Phytochemistry 69, no. 4: 857-864.
Targeting tumour neovasculature using antibodies to the endothelial receptor CD105 (endoglin), is a potentially useful approach for anti-tumour therapy. We report on the preparation and the cytotoxicity of a novel immunotoxin consisting in the non-toxic type 2 ribosome-inactivating protein (RIP) nigrin b linked to the monoclonal anti-human CD105 (hCD105) antibody 44G4. The immunotoxin kills specifically mouse fibroblasts expressing the biomarker CD105 (L929-hCD105+ cells) with an IC(50) value of 6x10(-10)M while nigrin b does it at 2.4x10(-7)M. Immunofluorescence analysis indicated that the immunotoxin accumulates in a perinuclear region. In contrast, 44G4 showed a specific localization on the cell surface.
Raquel Muñoz; Yolanda Arias; José M. Ferreras; Maria Angeles Rojo; Manuel J. Gayoso; Mercedes Nocito; Jorge Benítez; Pilar Jiménez; Carmelo Bernabeu; Tomás Girbés. Targeting a marker of the tumour neovasculature using a novel anti-human CD105-immunotoxin containing the non-toxic type 2 ribosome-inactivating protein nigrin b. Cancer Letters 2007, 256, 73 -80.
AMA StyleRaquel Muñoz, Yolanda Arias, José M. Ferreras, Maria Angeles Rojo, Manuel J. Gayoso, Mercedes Nocito, Jorge Benítez, Pilar Jiménez, Carmelo Bernabeu, Tomás Girbés. Targeting a marker of the tumour neovasculature using a novel anti-human CD105-immunotoxin containing the non-toxic type 2 ribosome-inactivating protein nigrin b. Cancer Letters. 2007; 256 (1):73-80.
Chicago/Turabian StyleRaquel Muñoz; Yolanda Arias; José M. Ferreras; Maria Angeles Rojo; Manuel J. Gayoso; Mercedes Nocito; Jorge Benítez; Pilar Jiménez; Carmelo Bernabeu; Tomás Girbés. 2007. "Targeting a marker of the tumour neovasculature using a novel anti-human CD105-immunotoxin containing the non-toxic type 2 ribosome-inactivating protein nigrin b." Cancer Letters 256, no. 1: 73-80.
Bark lectins from the elderberry plants belonging to the genus Sambucus specifically bind to Neu5Acα2,6Gal/GalNAc sequence and have long been used for the analysis of sialoglycoconjugates that play important roles in many biological phenomena. However, molecular basis of such a unique carbohydrate binding specificity has not been understood. To answer these questions, we tried to identify the amino-acid residues in the Japanese elderberry bark lectin, Sambucus sieboldiana agglutinin that enabled the lectin to recognize sialic acid by using in silico docking simulation and site-directed mutagenesis. These studies showed that three amino-acid residues, S197, A233 and Q234, in the C-terminal subdomain of SSA-B chain are critical for the binding to the sialic acid in Neu5Acα2,6Gal/GalNAc sequence. Replacement of one of these residues to the one in the corresponding position of ricin B-chain completely abolished the binding to a sialoglycoprotein, fetuin. Conserved presence of these amino acid residues in the corresponding sequences of two other elderberry lectins with similar binding specificity further supported the conclusion. These findings indicated that the replacement of the corresponding amino-acid residues in a putative Gal/GalNAc-specific ancestral lectin to these amino-acid residues generated the unique Neu5Acα2,6Gal/GalNAc-specific elderberry lectins in the course of molecular evolution.
Hanae Kaku; Hiroki Kaneko; Naoto Minamihara; Kazumichi Iwata; Elizabeth T. Jordan; Maria Angeles Rojo; Naoko Minami-Ishii; Eiichi Minami; Shigeru Hisajima; Naoto Shibuya. Elderberry Bark Lectins Evolved to Recognize Neu5Ac 2,6Gal/GalNAc Sequence from a Gal/GalNAc Binding Lectin Through the Substitution of Amino-Acid Residues Critical for the Binding to Sialic Acid. The Journal of Biochemistry 2007, 142, 393 -401.
AMA StyleHanae Kaku, Hiroki Kaneko, Naoto Minamihara, Kazumichi Iwata, Elizabeth T. Jordan, Maria Angeles Rojo, Naoko Minami-Ishii, Eiichi Minami, Shigeru Hisajima, Naoto Shibuya. Elderberry Bark Lectins Evolved to Recognize Neu5Ac 2,6Gal/GalNAc Sequence from a Gal/GalNAc Binding Lectin Through the Substitution of Amino-Acid Residues Critical for the Binding to Sialic Acid. The Journal of Biochemistry. 2007; 142 (3):393-401.
Chicago/Turabian StyleHanae Kaku; Hiroki Kaneko; Naoto Minamihara; Kazumichi Iwata; Elizabeth T. Jordan; Maria Angeles Rojo; Naoko Minami-Ishii; Eiichi Minami; Shigeru Hisajima; Naoto Shibuya. 2007. "Elderberry Bark Lectins Evolved to Recognize Neu5Ac 2,6Gal/GalNAc Sequence from a Gal/GalNAc Binding Lectin Through the Substitution of Amino-Acid Residues Critical for the Binding to Sialic Acid." The Journal of Biochemistry 142, no. 3: 393-401.
Nigrin b is a non-toxic type 2 ribosome-inactivating protein as active as ricin at ribosomal level but 105 and 5 × 103 times less toxic for animal cell cultures and mice, respectively, than ricin. The purpose of the present study was to analyze the effects of intravenous injection of large amounts of nigrin b to the mouse. Injection through the tail vein of 16 mg/kg body weight killed all mice studied before 2 days. Analysis of several major tissues by light microscopy did not reveal gross nigrin b-promoted changes, except in the intestines which appeared highly damaged. As a consequence of the injury, the villi and crypt structures of the small intestine disappeared, leading to profuse bleeding and death. In contrast, intravenous injection of 5 mg/kg body weight was not lethal to mice but did trigger reversible toxic effects. In both cases, lethal and sub-lethal doses, the target of nigrin b appeared to be the highly proliferating stem cells of the intestinal crypts, which had undergone apoptotic changes. In contrast to nigrin b, the injection of 3 μg/kg of ricin kills all mice in 5 days but does not trigger apoptosis in the crypts. Therefore, the effect seen with sub-lethal nigrin b concentrations seems to be specific. Nigrin b killed COLO 320 human colon adenocarcinoma cells with an IC50 of 3.1 × 10−8 M and the effect was parallel to the extent of DNA fragmentation of these cells. Accordingly, despite the low general toxicity exerted by nigrin b as compared with ricin, intravenous injection of large amounts of nigrin b is able to kill mouse intestinal stem cells without threatening the lives of the animals, thereby opening a door for its use for the targeting of intestinal stem cells.
Manuel Gayoso; Raquel Muñoz; Y Arias; R Villar; Maria Angeles Rojo; P Jimenez; J Ferreras; Isabel Aranguez; Tomas Girbes. Specific dose-dependent damage of Lieberkühn crypts promoted by large doses of type 2 ribosome-inactivating protein nigrin b intravenous injection to mice. Toxicology and Applied Pharmacology 2005, 207, 138 -146.
AMA StyleManuel Gayoso, Raquel Muñoz, Y Arias, R Villar, Maria Angeles Rojo, P Jimenez, J Ferreras, Isabel Aranguez, Tomas Girbes. Specific dose-dependent damage of Lieberkühn crypts promoted by large doses of type 2 ribosome-inactivating protein nigrin b intravenous injection to mice. Toxicology and Applied Pharmacology. 2005; 207 (2):138-146.
Chicago/Turabian StyleManuel Gayoso; Raquel Muñoz; Y Arias; R Villar; Maria Angeles Rojo; P Jimenez; J Ferreras; Isabel Aranguez; Tomas Girbes. 2005. "Specific dose-dependent damage of Lieberkühn crypts promoted by large doses of type 2 ribosome-inactivating protein nigrin b intravenous injection to mice." Toxicology and Applied Pharmacology 207, no. 2: 138-146.
Sugar beet (Beta vulgaris L.) leaves contain virus-inducible type 1 (single chain) ribosome-inactivating proteins that have been named beetins. The structural and functional characterization, the cellular location, and the potential role of beetins as antiviral agents are reported here. Beetins are formed of a single polypeptide chain with a varying degree of glycosylation and strongly inhibited in vitro protein synthesis in rabbit reticulocyte lysates (IC50=1.15 ng ml−1) and a Vicia sativa L. cell-free system (IC50=68 ng ml−1) through the single depurination of the large rRNA. Beetins trigger the multidepurination of tobacco mosaic virus (TMV) genomic RNA which underwent extensive degradation upon treatment with acid aniline. Beetins are extracellular proteins that were recovered from the apoplastic fluid. Induction of sugar beet RIPs with either H2O2 or artichoke mottled crinkle virus (AMCV) was observed in leaves distant from the site of application of such elicitors. The external application of purified beetin to sugar leaves prevented infection by AMCV which supports the preliminary hypothesis that beetins could be involved in plant systemic acquired resistance subjected to induction by phytopathogens.
Rosario Iglesias; Yolanda Pérez; Carlos de Torre; J. Miguel Ferreras; Pilar Antolín; Pilar Jiménez; Maria Angeles Rojo; Enrique Méndez; Tomás Girbés. Molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (RIPs) in sugar beet (Beta vulgaris) leaves. Journal of Experimental Botany 2005, 56, 1675 -1684.
AMA StyleRosario Iglesias, Yolanda Pérez, Carlos de Torre, J. Miguel Ferreras, Pilar Antolín, Pilar Jiménez, Maria Angeles Rojo, Enrique Méndez, Tomás Girbés. Molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (RIPs) in sugar beet (Beta vulgaris) leaves. Journal of Experimental Botany. 2005; 56 (416):1675-1684.
Chicago/Turabian StyleRosario Iglesias; Yolanda Pérez; Carlos de Torre; J. Miguel Ferreras; Pilar Antolín; Pilar Jiménez; Maria Angeles Rojo; Enrique Méndez; Tomás Girbés. 2005. "Molecular characterization and systemic induction of single-chain ribosome-inactivating proteins (RIPs) in sugar beet (Beta vulgaris) leaves." Journal of Experimental Botany 56, no. 416: 1675-1684.
Musarmins are type 1 ribosome-inactivating proteins with N-glycosidase activity on the 28 S rRNA that are present in bulbs of Muscari armeniacum L. and Miller at rather low concentrations. In the present work, a cDNA fragment coding for musarmin 1 was sub-cloned and expressed in Escherichia coli. The recombinant protein (rMU1) was synthesised as a polypeptide of 295 amino acids that was delivered to the periplasm and processed. Recombinant musarmin 1 present in the periplam has two forms: insoluble with a molecular mass of 29,423 and soluble with a molecular mass of 29,117 because of a small proteolytic shortening with respect to the insoluble one, presumably in the C-terminal. The yield of protein homogeneous by polyacrylamide gel electrophoresis was 23 mg l−1 of bacterial culture. The recombinant musarmin 1 forms isolated from both the soluble and the insoluble (upon refolding) fractions retained full translational inhibitory and 28 S rRNA N-glycosidase activities as compared with the native protein. The recombinant protein displayed great stability towards trypsin, collagenase, rat plasma and rat liver protein extract, but was sensitive to the action of papain and proteinase K. The easy availability and full activity of the recombinant musarmin 1 makes it a good candidate for the preparation of immunotoxins for targeted therapy and for the construction of transgenic plants expressing it as antipathogenic agent.
Pilar Antolı́n; Alessandra Girotti; Francisco Javier Arias; Begoña Barriuso; Pilar Jiménez; Ma Angeles Rojo; Tomás Girbés. Bacterial expression of biologically active recombinant musarmin 1 from bulbs of Muscari armeniacum L. and Miller. Journal of Biotechnology 2004, 112, 313 -322.
AMA StylePilar Antolı́n, Alessandra Girotti, Francisco Javier Arias, Begoña Barriuso, Pilar Jiménez, Ma Angeles Rojo, Tomás Girbés. Bacterial expression of biologically active recombinant musarmin 1 from bulbs of Muscari armeniacum L. and Miller. Journal of Biotechnology. 2004; 112 (3):313-322.
Chicago/Turabian StylePilar Antolı́n; Alessandra Girotti; Francisco Javier Arias; Begoña Barriuso; Pilar Jiménez; Ma Angeles Rojo; Tomás Girbés. 2004. "Bacterial expression of biologically active recombinant musarmin 1 from bulbs of Muscari armeniacum L. and Miller." Journal of Biotechnology 112, no. 3: 313-322.
Two monomeric lectins, SSA-b-3 and SSA-b-4, were purified from the bark tissue of Japanese elderberry, Sambucus sieboldiana. SDS-PAGE of the purified lectins showed the presence of single bands of 35 and 33 kDa for SSA-b-3 and SSA-b-4, respectively, irrespective of the presence of reducing agent. MS analysis as well as gel filtration of these lectins indicated that they exist mostly as monomeric lectins. Analysis of the N-terminal amino acid sequences of SSA-b-3 and SSA-b-4 yielded an identical sequence, indicating their close structural relationship. Four cDNA clones with extensive homology were obtained from the bark cDNA library and indicated to encode SSA-b-3 or SSA-b-4 from the comparison with the N-terminal sequences of these lectins. These clones were classified into two groups, three for SSA-b-3 and one for SSA-b-4, based on the predicted isoelectric points. The amino acid sequences of the encoded polypeptides were almost identical with the B-chain of a type 2 ribosome-inactivating protein from the same bark tissue, sieboldin-b, except for the absence of a small peptide containing a cystein residue, which is critical for the heteromeric dimerization with an A-subunit. Carbohydrate binding specificity and biological activity of these lectins are also reported.
Maria Angeles Rojo; Hanae Kaku; Naoko Ishii-Minami; Eiichi Minami; Misa Yato; Shigeru Hisajima; Takeshi Yamaguchi; Naoto Shibuya. Characterization and cDNA cloning of monomeric lectins that correspond to the B-Chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana). The Journal of Biochemistry 2004, 135, 509 -516.
AMA StyleMaria Angeles Rojo, Hanae Kaku, Naoko Ishii-Minami, Eiichi Minami, Misa Yato, Shigeru Hisajima, Takeshi Yamaguchi, Naoto Shibuya. Characterization and cDNA cloning of monomeric lectins that correspond to the B-Chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana). The Journal of Biochemistry. 2004; 135 (4):509-516.
Chicago/Turabian StyleMaria Angeles Rojo; Hanae Kaku; Naoko Ishii-Minami; Eiichi Minami; Misa Yato; Shigeru Hisajima; Takeshi Yamaguchi; Naoto Shibuya. 2004. "Characterization and cDNA cloning of monomeric lectins that correspond to the B-Chain of a type 2 ribosome-inactivating protein from the bark of Japanese elderberry (Sambucus sieboldiana)." The Journal of Biochemistry 135, no. 4: 509-516.
SELld is a dimeric d-galactose and mucin-binding lectin (apparent Mr 68,000) which coexists with the non-toxic type 2 ribosome-inactivating protein (RIP) ebulin l in dwarf elder (Sambucus ebulus L.) leaves. To ascertain a potential structural correlation with ebulin l molecular cloning of a cDNA coding for SELld was performed. SELld shared a 76% of identity with the ebulin l-B chain. Notably, it was found that SELld has Tyr present in the high affinity 2γ sugar-binding domain of ricin which is absent in ebulin l-B chain and which seems responsible of the low cell and in vivo toxicities of ebulin l. The concentration of ebulin l in leaves decreased along the developmental stage of dwarf elder and almost disappeared in senescence while the content in SELld changed in the opposite way. Our results suggest that SELld and ebulin l play different biological roles in dwarf elder leaves.
Maria Angeles Rojo. cDNA molecular cloning and seasonal acumulation of an ebulin l-related dimeric lectin of dwarf elder (Sambucus ebulus L.) leaves. The International Journal of Biochemistry & Cell Biology 2003, 35, 1061 -1065.
AMA StyleMaria Angeles Rojo. cDNA molecular cloning and seasonal acumulation of an ebulin l-related dimeric lectin of dwarf elder (Sambucus ebulus L.) leaves. The International Journal of Biochemistry & Cell Biology. 2003; 35 (7):1061-1065.
Chicago/Turabian StyleMaria Angeles Rojo. 2003. "cDNA molecular cloning and seasonal acumulation of an ebulin l-related dimeric lectin of dwarf elder (Sambucus ebulus L.) leaves." The International Journal of Biochemistry & Cell Biology 35, no. 7: 1061-1065.