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The simultaneous quantification of trace and micro metabolites is a bottleneck in food and biological analysis. Phenolic compounds are the most widely distributed and have various physiological functions. In this study, the strategy for the simultaneous liquid chromatography tandem-mass spectrometry (LC-MS/MS) quantification of 13 trace and micro phenolic compounds was proposed by taking product ions and isotopic ions as quantitative ions. The method validation results showed that the limits of detection (LODs) were from 0.01 to 9.84 μg/kg, and the limits of quantification (LOQs) were from 0.03 to 32.8 μg/kg. The intra-day precision and inter-day precision were below 8.4% and 14.4%, respectively. The recoveries ranged from 81.9% to 117.2%, and the matrix effects ranged from −11.5% to 13.7%, which indicated that the method has high sensitivity and suitable stability. The developed analytical method was applied to determine trace and micro constituents in rapeseed samples. The analysis results indicated that the contents of sinapine have significantly different between high and low total phenolic content rapeseeds. This method provides a reference strategy for the simultaneous quantitative analysis of other micro- and trace antioxidants.
Dandan Wang; Liangxiao Zhang; Li Yu; Fei Ma; Peiwu Li. Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry. Metabolites 2021, 11, 589 .
AMA StyleDandan Wang, Liangxiao Zhang, Li Yu, Fei Ma, Peiwu Li. Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry. Metabolites. 2021; 11 (9):589.
Chicago/Turabian StyleDandan Wang; Liangxiao Zhang; Li Yu; Fei Ma; Peiwu Li. 2021. "Simultaneous Quantification of Trace and Micro Phenolic Compounds by Liquid Chromatography Tandem-Mass Spectrometry." Metabolites 11, no. 9: 589.
An ultrasensitive DNA biosensor based on the fluorescence quenching ability of FOQDs towards FAM–ssDNA through π–π stacking interactions between ssDNA and FOQDs.
Hui Li; Hua Pang; Liangxiao Zhang; Jin Mao; Wen Zhang; Jun Jiang; Peiwu Li; Qi Zhang. Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots. RSC Advances 2021, 11, 19690 -19694.
AMA StyleHui Li, Hua Pang, Liangxiao Zhang, Jin Mao, Wen Zhang, Jun Jiang, Peiwu Li, Qi Zhang. Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots. RSC Advances. 2021; 11 (32):19690-19694.
Chicago/Turabian StyleHui Li; Hua Pang; Liangxiao Zhang; Jin Mao; Wen Zhang; Jun Jiang; Peiwu Li; Qi Zhang. 2021. "Ultrasensitive biosensing platform based on luminescence quenching ability of fullerenol quantum dots." RSC Advances 11, no. 32: 19690-19694.
A multicolor enzyme-linked immunosorbent sensor was constructed to detect organophosphates pesticides (OPs) on base of TMB2+-mediated AuNRs etching. As the etching progresses, a rainbow-like color responses was generated. The naked eye limit of detection (LOD) of the sensing system is 0.25 ng mL−1. In particular, the longitudinal localized surface plasmon resonances (LSPR) peak shift of AuNRs is linearly correlated with the logarithm of OPs concentration in range of 104 to 5 × 106 pg mL−1 for parathion-methyl and fenitrothion, 5 × 104 to 107 pg mL−1 for fenthion, respectively. Compared with reported methods, this sensor displayed satisfactory linear ranges and detection limits. Average recoveries of this method ranged from 73.28% to 98.08% and relative standard deviations (RSDs) were below 8.0% as for food samples analysis without complicated pretreatments. Remarkably, the developed multicolor enzyme-linked immunosorbent sensor is capable of providing accurate and reliable assay for OPs in complex food samples.
Xiao-Li Yin; Yun Qing Liu; Hui-Wen Gu; Qi Zhang; Zhao Wei Zhang; Hui Li; Pei-Wu Li; Yu Zhou. Multicolor enzyme-linked immunosorbent sensor for sensitive detection of organophosphorus pesticides based on TMB2+-mediated etching of gold nanorods. Microchemical Journal 2021, 168, 106411 .
AMA StyleXiao-Li Yin, Yun Qing Liu, Hui-Wen Gu, Qi Zhang, Zhao Wei Zhang, Hui Li, Pei-Wu Li, Yu Zhou. Multicolor enzyme-linked immunosorbent sensor for sensitive detection of organophosphorus pesticides based on TMB2+-mediated etching of gold nanorods. Microchemical Journal. 2021; 168 ():106411.
Chicago/Turabian StyleXiao-Li Yin; Yun Qing Liu; Hui-Wen Gu; Qi Zhang; Zhao Wei Zhang; Hui Li; Pei-Wu Li; Yu Zhou. 2021. "Multicolor enzyme-linked immunosorbent sensor for sensitive detection of organophosphorus pesticides based on TMB2+-mediated etching of gold nanorods." Microchemical Journal 168, no. : 106411.
Biocontrol to combat the menace of Aspergillus flavus has gained considerable attention. However, the molecular mechanisms of A. flavus ’s response to antagonism biotic stress are poorly deciphered. Here, we discovered that A. flavus switches an adaptive metabolic reprogramming to ensure its adversity survival by multiomics analyses (including four omics platform). Antifungal “weapons” lipopeptides and antibacterial metabolites of imizoquin were identified. The central metabolism fluxes were significantly depleted but the expressions of most corresponding genes were considerably increased in A. flavus. Secondary metabolism that does not contribute to stress was markedly suppressed. In contrast, A. flavus antibacterial “weapon arsenal” was activated to occupy an ecological niche. Our results revealed that interlinked mitochondrial central metabolism and secondary metabolism are central to A. flavus antagonism biotic stress response. This discovery contributes to the targeted design of biocontrol agents and smart regularization of rhizosphere microbiome homeostasis to realize long-term fungi pathogen control and mitigation mycotoxin contamination.
Huali Xie; Abdoulie Jallow; Xiaofeng Yue; Xiuping Wang; Jiayun Fu; Silvano E. Mwakinyali; Qi Zhang; Peiwu Li. Aspergillus flavus’s Response to Antagonism Bacterial Stress Sheds Light on a Regulation and Metabolic Trade-Off Mechanism for Adversity Survival. Journal of Agricultural and Food Chemistry 2021, 69, 4840 -4848.
AMA StyleHuali Xie, Abdoulie Jallow, Xiaofeng Yue, Xiuping Wang, Jiayun Fu, Silvano E. Mwakinyali, Qi Zhang, Peiwu Li. Aspergillus flavus’s Response to Antagonism Bacterial Stress Sheds Light on a Regulation and Metabolic Trade-Off Mechanism for Adversity Survival. Journal of Agricultural and Food Chemistry. 2021; 69 (16):4840-4848.
Chicago/Turabian StyleHuali Xie; Abdoulie Jallow; Xiaofeng Yue; Xiuping Wang; Jiayun Fu; Silvano E. Mwakinyali; Qi Zhang; Peiwu Li. 2021. "Aspergillus flavus’s Response to Antagonism Bacterial Stress Sheds Light on a Regulation and Metabolic Trade-Off Mechanism for Adversity Survival." Journal of Agricultural and Food Chemistry 69, no. 16: 4840-4848.
Aspergillus flavus can cause crop loss and produce mycotoxin that bring serious threats to human health. The large usage of chemical pesticides or fungicides might cause environmental problems and fungal resistance. Therefore, developing the novel, low possibility of resistance and pro-environment way to control and reduce this fungi is meaningful. Herein, an efficient, environmental-friendly and photocatalytic method to disinfect Aspergillus flavus was demonstrated on magnetic and Z-schematic composites. The composites were composed of rhombic NiFe2O4 nanosheets and g-C3N4 nanosized sheets, and 0.2 g-C3N4/NiFe2O4 (mass ratio) showed the excellent activity with above 90% disinfection rate under 90 min visible light irradiation. The efficient disinfection activities were ascribed to the favorable charges separation, well photoelectric properties and suitable band structure. It was found that the •O2− and •OH were the main active radical species in photocatalytic disinfection. The attack of these species could cause the destruction of fungal cell structure and the leakage of intracellular contents as proved by the fluorescence staining of spores, biological scanning electron microscope and the detection of extracellular protein and DNA. Moreover, the composites also showed high photocatalytic disinfection activity of Aspergillus flavus on peanuts, which can reduce aflatoxin B1 from 315.21 µg kg-1 to 18.50 µg kg-1. This efficient method might be considered as a potential way to control the contamination of Aspergillus flavus, and as a reference to the mitigation of other pathogenic fungi in future.
Di Sun; Jin Mao; Ling Cheng; Xianglong Yang; Hui Li; Liangxiao Zhang; Wen Zhang; Qi Zhang; Peiwu Li. Magnetic g-C3N4/NiFe2O4 composite with enhanced activity on photocatalytic disinfection of Aspergillus flavus. Chemical Engineering Journal 2021, 418, 129417 .
AMA StyleDi Sun, Jin Mao, Ling Cheng, Xianglong Yang, Hui Li, Liangxiao Zhang, Wen Zhang, Qi Zhang, Peiwu Li. Magnetic g-C3N4/NiFe2O4 composite with enhanced activity on photocatalytic disinfection of Aspergillus flavus. Chemical Engineering Journal. 2021; 418 ():129417.
Chicago/Turabian StyleDi Sun; Jin Mao; Ling Cheng; Xianglong Yang; Hui Li; Liangxiao Zhang; Wen Zhang; Qi Zhang; Peiwu Li. 2021. "Magnetic g-C3N4/NiFe2O4 composite with enhanced activity on photocatalytic disinfection of Aspergillus flavus." Chemical Engineering Journal 418, no. : 129417.
Food safety is currently a significant issue for human life and health. Various fluorescent nanomaterials have been applied in the point-of-care test (POCT) for food safety as labeling materials. However, previous fluorescent nanomaterials can cause aggregation-caused quenching (ACQ), thus reducing the detection sensitivity. Conversely, aggregation-induced emission luminogens (AIEgens) are promising candidates for POCT in the food safety field because they can enhance detection sensitivity and throughput. Mycotoxins, such as aflatoxin B1 (AFB1) and cyclopiazonic acid (CPA), are a primary threat to human life and health and a significant food safety issue, and their on-site detection from farm to table is needed. Herein, an ultrasensitive point-of-care test was developed based on TPE-Br, a blue-emissive tetraphenylethylene derivative AIEgen. Under optimal conditions, this AIEgen-based lateral-flow biosensor (ALFB) allowed for a rapid response of 8 min toward AFB1 and CPA detection, with considerable sensitivities of 0.003 and 0.01 ng/mL in peanut matrices, respectively. In peanut matrices, the recoveries were 90.3%-110.0% for both mycotoxins, with relative standard deviations (RSDs) below 6%. The ALFB was further validated via UPLC-MS/MS using spiked peanut samples. AIEgens open an avenue for on-site, ultrasensitive, high-throughput detection methods and can be extensively used in point-of-care tests in food safety.
Xiaofeng Hu; Pengfei Zhang; Du Wang; Jun Jiang; Xiaomei Chen; Yong Liu; Zhaowei Zhang; Ben Zhong Tang; Peiwu Li. AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B1 and cyclopiazonic acid as an example. Biosensors and Bioelectronics 2021, 182, 113188 .
AMA StyleXiaofeng Hu, Pengfei Zhang, Du Wang, Jun Jiang, Xiaomei Chen, Yong Liu, Zhaowei Zhang, Ben Zhong Tang, Peiwu Li. AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B1 and cyclopiazonic acid as an example. Biosensors and Bioelectronics. 2021; 182 ():113188.
Chicago/Turabian StyleXiaofeng Hu; Pengfei Zhang; Du Wang; Jun Jiang; Xiaomei Chen; Yong Liu; Zhaowei Zhang; Ben Zhong Tang; Peiwu Li. 2021. "AIEgens enabled ultrasensitive point-of-care test for multiple targets of food safety: Aflatoxin B1 and cyclopiazonic acid as an example." Biosensors and Bioelectronics 182, no. : 113188.
Aflatoxin B1 is a strong carcinogenic and toxic fungal toxin produced by Aspergillus flavus and other Aspergillus species, and can seriously threaten the health of consumers and the safety and quality of agricultural products. Aspergillus in agricultural products are closely related to topography and symbiotic microbes. It is not fully clear that how topography affects the assembly process of A. flavus and symbiotic fungi on plant. In this study, we analyzed the structure and assembly process of fungi on the peanut. We also performed the metatranscriptome analysis, identified the functional genes and metabolic pathways enriched in both A. flavus and its symbiotic fungi. In our experiment, terrain and soil properties could significantly affect the gene expression of microbiome, A. flavus abundance and infection ability to peanuts. Meanwhile, the Permanova correlation analysis revealed that differentially expressed genes were strongly correlated with the soil physicochemical factors. Furthermore, metabolomic analysis identified the main metabolites associated with A. flavus and aflatoxin B1, the results proved that the terrain significantly affected the microorganisms associated with peanut pods to produce a variety of metabolites. In conclusion, our results indicate that topography can significantly influence the assembly process of A. flavus and microorganisms, the activation of functional genes and metabolic pathways, the enrichment of aflatoxin-producing fungi.
Yanpo Yao; Suyan Gao; Xiaoxia Ding; Qi Zhang; Peiwu Li. Topography effect on Aspergillus flavus occurrence and aflatoxin B1 contamination associated with peanut. Current Research in Microbial Sciences 2021, 2, 100021 .
AMA StyleYanpo Yao, Suyan Gao, Xiaoxia Ding, Qi Zhang, Peiwu Li. Topography effect on Aspergillus flavus occurrence and aflatoxin B1 contamination associated with peanut. Current Research in Microbial Sciences. 2021; 2 ():100021.
Chicago/Turabian StyleYanpo Yao; Suyan Gao; Xiaoxia Ding; Qi Zhang; Peiwu Li. 2021. "Topography effect on Aspergillus flavus occurrence and aflatoxin B1 contamination associated with peanut." Current Research in Microbial Sciences 2, no. : 100021.
To simultaneously detect two metabolites of Aspergillus flavus, namely, cyclopiazonic acid (CPA) and aflatoxin (AFT), an ultrasensitive monoclonal antibody (mAb) YTT-2 against CPA was developed and characterized, with sensitivity to CPA of 1.32 ng mL−1. Along with the previously homemade mAb 1C11 against AFT, two mAbs were used to develop time-resolved fluorescence immunoprobes or gold immunoprobes. We developed two multiple-analyte paper immunosensors including time-resolved fluorescent immunochromatographic assay (TRFICA) and gold immunochromatographic assay (GICA) for the simultaneous determination of CPA and AFT. The TRFICA showed limits of determination (LODs) of 0.21 and 0.004 ng mL−1, while the GICA showed LODs of 0.33 and 0.01 ng mL−1 for CPA and AFT, respectively. To validate the specificity of the two rapid immunoassays, rice, corn and peanut samples were spiked with different concentrations of CPA and AFT. The two methods showed satisfactory recoveries (76.39~90.82% for CPA and 84.60~94.45% for AFT) and coefficients of variation of 3.50~7.80% for CPA and 4.12~13.90% for AFT. The results indicated that the TRFICA could complete the test within 5 min and had lower LODs and linear ranges, compared with that of GICA. The method developed in this work can be widely applied to the rapid and quantitative simultaneous determination of multiple harmful metabolites in fungi for food safety and health care. Graphical abstract
Tingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta 2020, 187, 1 -8.
AMA StyleTingting Yan, Zhaowei Zhang, Qi Zhang, Xiaoqian Tang, Du Wang, Xiaofeng Hu, Wen Zhang, Xiaomei Chen, Peiwu Li. Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies. Microchimica Acta. 2020; 187 (12):1-8.
Chicago/Turabian StyleTingting Yan; Zhaowei Zhang; Qi Zhang; Xiaoqian Tang; Du Wang; Xiaofeng Hu; Wen Zhang; Xiaomei Chen; Peiwu Li. 2020. "Simultaneous determination for A. flavus–metabolizing mycotoxins by time-resolved fluorescent microbead or gold-enabling test strip in agricultural products based on monoclonal antibodies." Microchimica Acta 187, no. 12: 1-8.
Ochratoxin A (OTA) is a known food contaminant that affects a wide range of food and agricultural products. The presence of this fungal metabolite in foods poses a threat to human health. Therefore, various detection and quantification methods have been developed to determine its presence in foods. Herein, we describe a rapid and ultrasensitive tracer-based fluorescence polarization immunoassay (FPIA) for the detection of OTA in rice samples. Four fluorescent tracers OTA-fluorescein thiocarbamoyl ethylenediamine (EDF), OTA-fluorescein thiocarbamoyl butane diamine (BDF), OTA-amino-methyl fluorescein (AMF), and OTA-fluorescein thiocarbamoyl hexame (HDF) with fluorescence polarization values (δFP = FPbind-FPfree) of 5, 100, 207, and 80 mP, respectively, were synthesized. The tracer with the highest δFP value (OTA-AMF) was selected and further optimized for the development of an ultrasensitive FPIA with a detection range of 0.03–0.78 ng/mL. A mean recovery of 70.0% to 110.0% was obtained from spiked rice samples with a relative standard deviation of equal to or less than 20%. Good correlations (r2 = 0.9966) were observed between OTA levels in contaminated rice samples obtained by the FPIA method and high-performance liquid chromatography (HPLC) as a reference method. The rapidity of the method was confirmed by analyzing ten rice samples that were analyzed within 25 min, on average. The sensitivity, accuracy, and rapidity of the method show that it is suitable for screening and quantification of OTA in food samples without the cumbersome pre-analytical steps required in other mycotoxin detection methods.
Xiaorong Huang; Xiaoqian Tang; Abdoulie Jallow; Xin Qi; Wen Zhang; Jun Jiang; Hui Li; Qi Zhang; Peiwu Li. Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice. Toxins 2020, 12, 682 .
AMA StyleXiaorong Huang, Xiaoqian Tang, Abdoulie Jallow, Xin Qi, Wen Zhang, Jun Jiang, Hui Li, Qi Zhang, Peiwu Li. Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice. Toxins. 2020; 12 (11):682.
Chicago/Turabian StyleXiaorong Huang; Xiaoqian Tang; Abdoulie Jallow; Xin Qi; Wen Zhang; Jun Jiang; Hui Li; Qi Zhang; Peiwu Li. 2020. "Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice." Toxins 12, no. 11: 682.
A wide variety of mycotoxins is produced by mycotoxigenic fungi and naturally contaminates food and feed products worldwide. Synergistic effects of multi-toxins are potentially more harmful than exposure to a single compound and can induce acute and chronic toxicity to animals and humans. The aim of the present study is to timely and simultaneously identify the multiple mycotoxigenic fungi capable of causing synergistic toxicity to improve the safety level of food and feedstuff. Here, a multiplex polymerase chain reaction assay was developed for simultaneous detection of mycotoxigenic fungi belonging to the genera Aspergillus, Fusarium and Penicillium. Three pairs of genus-specific primers were designed based on internal transcribed spacer (ITS) sequences of Aspergillus and Penicillium, and Elongation factor 1 alpha (EF- 1α) of Fusarium. Amplicons of 170, 750 and 490 bp respectively for the corresponding primer pairs were detected; thus amplicon length is diagnostic for the individual fungal genus. The sensitivity of the developed method was tested with genomic DNA obtained from mould pure cultures and artificially contaminated maize grain powder. The sensitivity result showed that spore concentrations in the contaminated maize grain powder of 102 spores/mL were detected without prior incubation. This result suggests that the developed mPCR assay would allow a rapid, specific and simultaneous detection of various mycotoxigenic potential fungi based on the occurrence and size of the amplification products and thus to estimate the multi-mycotoxins contamination potential in food and feedstuff.
Hamid Ur Rahman; Xiaofeng Yue; Xianfeng Ren; Wen Zhang; Qi Zhang; Peiwu Li. Multiplex PCR assay to detect Aspergillus, Penicillium and Fusarium species simultaneously. Food Additives & Contaminants: Part A 2020, 37, 1939 -1950.
AMA StyleHamid Ur Rahman, Xiaofeng Yue, Xianfeng Ren, Wen Zhang, Qi Zhang, Peiwu Li. Multiplex PCR assay to detect Aspergillus, Penicillium and Fusarium species simultaneously. Food Additives & Contaminants: Part A. 2020; 37 (11):1939-1950.
Chicago/Turabian StyleHamid Ur Rahman; Xiaofeng Yue; Xianfeng Ren; Wen Zhang; Qi Zhang; Peiwu Li. 2020. "Multiplex PCR assay to detect Aspergillus, Penicillium and Fusarium species simultaneously." Food Additives & Contaminants: Part A 37, no. 11: 1939-1950.
Edible oils play a vital role in our daily life, which provide human beings with energy, essential fatty acids and nutrients. The quality of edible oils can be dependent on the quality of the oilseeds they originate from, and their adulteration during processing. In recent years, near infrared spectroscopy was widely used in the rapid assessment of quality of oilseeds and edible oils. However, to the best of our knowledge, a comprehensive review on near infrared spectroscopy for the quality of oilseeds remains to be published. The applications of near infrared spectroscopy in the quality of oilseeds and edible oils have been emphasized in this review. This article briefly summarizes the basic knowledge of near infrared spectroscopy. In addition, we highlight the application of this technique on the detection of physicochemical properties and quality, specific nutrient components, authentication, and geographical origin traceability of oilseeds and edible oils. Moreover, the application of near infrared hyperspectral imaging technology in oilseeds has been addressed. Key findings and conclusions: Near infrared spectroscopy possesses the advantages of rapid, green and low-cost analysis. Meanwhile, it is also a nondestructive method and therefore suitable to quality analysis of oilseeds in agricultural sciences. It can be used to detect macro nutrients in oilseeds and edible oils by combining NIR and advanced chemometrics. In the future, NIR could be applied in the rapid detection and online detection of hazardous substances and nutrients in oilseeds by developing the new instrument and chemometric methods.
Xue Li; Liangxiao Zhang; Yong Zhang; Du Wang; Xuefang Wang; Li Yu; Wen Zhang; Peiwu Li. Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils. Trends in Food Science & Technology 2020, 101, 172 -181.
AMA StyleXue Li, Liangxiao Zhang, Yong Zhang, Du Wang, Xuefang Wang, Li Yu, Wen Zhang, Peiwu Li. Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils. Trends in Food Science & Technology. 2020; 101 ():172-181.
Chicago/Turabian StyleXue Li; Liangxiao Zhang; Yong Zhang; Du Wang; Xuefang Wang; Li Yu; Wen Zhang; Peiwu Li. 2020. "Review of NIR spectroscopy methods for nondestructive quality analysis of oilseeds and edible oils." Trends in Food Science & Technology 101, no. : 172-181.
Rapeseed (Brassica napus L.) is rich in phenols, vitamins, carotenoids, and mineral elements, such as selenium. Additionally, it contains the active ingredients sulforaphane and indole-3-carbinol, which have been demonstrated to have pharmacological effects. In this study, sulforaphane and indole-3-carbinol were extracted and quantified from rapeseeds using quick, easy, cheap, effective, rugged and safe (QuEChERS) method coupled with ultra high performance liquid chromarography tandem mass spectrometry (UHPLC-MS/MS). The major parameters for extraction and purification efficiency were optimized, including the hydrolysis reaction, extraction condition and type and amount of purification adsorbents. The limit of detection (LOD) and the limit of quantification (LOQ) for sulforaphane were 0.05 μg/kg and 0.15 μg/kg, and for indole-3-carbinol were 5 μg/kg and 15 μg/kg, respectively. The developed method was used to successfully analyze fifty rapeseed samples. The QuEChERS coupled with UHPLC-MS/MS simultaneously detect sulforaphane and indole-3-carbinol in vegetable matrix and evaluate the quality and nutrition of rapeseed samples.
Xu Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS. Molecules 2020, 25, 2149 .
AMA StyleXu Yu, Fei Ma, Liangxiao Zhang, Peiwu Li. Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS. Molecules. 2020; 25 (9):2149.
Chicago/Turabian StyleXu Yu; Fei Ma; Liangxiao Zhang; Peiwu Li. 2020. "Extraction and Quantification of Sulforaphane and Indole-3-Carbinol from Rapeseed Tissues Using QuEChERS Coupled with UHPLC-MS/MS." Molecules 25, no. 9: 2149.
A reliable, sensitive and accurate multiple mycotoxin method was developed for the simultaneous determination of 17 mycotoxins in swine, poultry and dairy feeds using stable isotope dilution (13C-ISTD) and (ultra)-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). A simple QuEChERS-based method (quick, easy, cheap, effective, rugged and safe) was developed consisting of soaking with a solution of 1% formic acid followed by extraction with acetonitrile, clean-up with C18 sorbent and finally adding 13C-ISTD before the UHPLC-MS/MS analysis. The chromatographic condition was optimized for separation and detection of the 17 mycotoxins using gradient elution. The method’s performance complied with the SANTE/11813/2017 standard and had mean recovery accuracies in the range 70%–120% and precision testing of % relative standard deviation (RSD) ≤ 20%. The limit of detection and limit of quantification values ranged from 0.25 to 40.0 ng/g and 0.5 to 100.0 ng/g, respectively. Finally, the method was applied to analyze feed samples, with the results showing that fumonisins, zearalenone, aflatoxin B1 and deoxynivalenol were the most prevalent mycotoxins contaminating the feed samples.
Kraiwut Nualkaw; Saranya Poapolathep; Zhaowei Zhang; Qi Zhang; Mario Giorgi; Peiwu Li; Antonio Francesco Logrieco; Amnart Poapolathep. Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry. Toxins 2020, 12, 253 .
AMA StyleKraiwut Nualkaw, Saranya Poapolathep, Zhaowei Zhang, Qi Zhang, Mario Giorgi, Peiwu Li, Antonio Francesco Logrieco, Amnart Poapolathep. Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry. Toxins. 2020; 12 (4):253.
Chicago/Turabian StyleKraiwut Nualkaw; Saranya Poapolathep; Zhaowei Zhang; Qi Zhang; Mario Giorgi; Peiwu Li; Antonio Francesco Logrieco; Amnart Poapolathep. 2020. "Simultaneous Determination of Multiple Mycotoxins in Swine, Poultry and Dairy Feeds Using Ultra High Performance Liquid Chromatography-Tandem Mass Spectrometry." Toxins 12, no. 4: 253.
Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized [email protected] and [email protected] conjugated with a second antibody ([email protected]). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. [email protected] was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety.
Weiqi Zhang; Wenqin Wu; Chong Cai; Xiaofeng Hu; Hui Li; Yizhen Bai; Zhaowei Zhang; Peiwu Li. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins 2020, 12, 158 .
AMA StyleWeiqi Zhang, Wenqin Wu, Chong Cai, Xiaofeng Hu, Hui Li, Yizhen Bai, Zhaowei Zhang, Peiwu Li. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins. 2020; 12 (3):158.
Chicago/Turabian StyleWeiqi Zhang; Wenqin Wu; Chong Cai; Xiaofeng Hu; Hui Li; Yizhen Bai; Zhaowei Zhang; Peiwu Li. 2020. "A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products." Toxins 12, no. 3: 158.
Food adulteration is a challenge faced by consumers and researchers. Due to DNA fragmentation during oil processing, it is necessary to discover metabolic markers alternative to DNA for adulteration detection of edible oils. However, the contents of metabolic markers vary in response to various factors, such as plant species, varieties, geographical origin, climate, and cultivation measures. Thus, it is difficult to identify a universal marker for all adulterants that may be present in some authentic samples. Currently, the specificity and selectivity of metabolic biomarkers are difficult to validate. Therefore, this study developed a screening strategy based on plant metabolic networks by developing a targeted analytical method for 56 metabolites in a metabolic network, using liquid/liquid extraction–liquid chromatography–tandem mass spectrometry (LC-MS/MS). We identified a chain of 11 metabolites that were related to isoflavonoid biosynthesis, which were detected in soybean oils but not rapeseed oils. Through multiple-marker mutual validation, these metabolites can be used as species-specific universal markers to differentiate soybean oil from rapeseed oil. Moreover, this method provides a model for screening characteristic markers of other edible vegetable oils and foods.
Xinjing Dou; Liangxiao Zhang; Xiao Wang; Ruinan Yang; Xuefang Wang; Fei Ma; Li Yu; Jin Mao; Hui Li; Xiupin Wang; Peiwu Li. Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks. Metabolites 2020, 10, 85 .
AMA StyleXinjing Dou, Liangxiao Zhang, Xiao Wang, Ruinan Yang, Xuefang Wang, Fei Ma, Li Yu, Jin Mao, Hui Li, Xiupin Wang, Peiwu Li. Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks. Metabolites. 2020; 10 (3):85.
Chicago/Turabian StyleXinjing Dou; Liangxiao Zhang; Xiao Wang; Ruinan Yang; Xuefang Wang; Fei Ma; Li Yu; Jin Mao; Hui Li; Xiupin Wang; Peiwu Li. 2020. "Identification and Validation of Metabolic Markers for Adulteration Detection of Edible Oils Using Metabolic Networks." Metabolites 10, no. 3: 85.
Vitamin K1 is one of the important hydrophobic vitamins in fat-containing foods. Traditionally, lipase is employed in the determination of vitamin K1 to remove the lipids, which makes the detection complex, time-consuming, and insensitive. In this study, the determination of vitamin K1 in fat-containing foods was developed based on ultrasound-assisted extraction (UAE), solid-phase extraction (SPE) combined with liquid chromatography–tandem mass spectrometry (LC-MS/MS). The optimal conditions for extraction of vitamin K1 were material–liquid ratio of 1:70 (g/mL), extraction temperature of 50 °C, extraction power of 700 W, extraction time of 50 min, material-wash fluid ratio of 1:60 (g/mL), and 8 mL of hexane/anhydrous ether (97:3, v/v) as the elution solvent. Then, vitamin K1 was analyzed on a ZORBAX SB-C18 column (50 mm × 2.1 mm, 1.8 μm) by gradient elution with water (0.01% formic acid) and methanol (0.01 formic acid + 2.5 mmol/L ammonium formate) as the mobile phase. The limit of detection (LOD) and limit of quantification (LOQ) were 0.05 and 0.16 μg/kg, respectively. Calibration curve was linear over the range of 10–500 ng/mL (R2 > 0.9988). The recoveries at three spiked levels were between 80.9% and 119.1%. The validation and application indicated that the proposed method was simple and sensitive in determination of vitamin K1 in fat-containing foods.
Yueqing Xu; Liangxiao Zhang; Ruinan Yang; Xu Yu; Li Yu; Fei Ma; Hui Li; Xiupin Wang; Peiwu Li. Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS. Molecules 2020, 25, 839 .
AMA StyleYueqing Xu, Liangxiao Zhang, Ruinan Yang, Xu Yu, Li Yu, Fei Ma, Hui Li, Xiupin Wang, Peiwu Li. Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS. Molecules. 2020; 25 (4):839.
Chicago/Turabian StyleYueqing Xu; Liangxiao Zhang; Ruinan Yang; Xu Yu; Li Yu; Fei Ma; Hui Li; Xiupin Wang; Peiwu Li. 2020. "Extraction and Determination of Vitamin K1 in Foods by Ultrasound-Assisted Extraction, SPE, and LC-MS/MS." Molecules 25, no. 4: 839.
Fumonisins (FBs) exist widely in crops, foods and feeds. Consumption of FBs contaminated corn can cause oesophageal cancer. So it is necessary to develop sensitivity methods for its detection. Here, we report an enhanced assay for rapid and ultra-sensitive detection of FBs based on nanomagnetic beads (NMBs) and antibody-biotin-streptavidin-HRP. Because antibody-BNHS can bind with several number of streptavidin-HRP, the signal amplification of the catalytical oxidation of TMB was enhanced. The detection limit of sensor was down to 0.21 ng mL−1 with a linear range from 0.31 to 162.42 ng mL−1. Since NMBs provide a nearly “in solution” reaction, they lead to a shortened reaction time (22 min) than that of flat solid-phase based traditional assay. Furthermore, the recoveries obtained by standard FBs spiked to maize samples were from 100.6 to 107.3%. This enhanced assay supplied a rapid, sensitive and reliable method for detection of FBs in maize.
Hualin Yang; Qi Zhang; Xiaolei Liu; Yuying Yang; Yong Yang; Mingyuan Liu; Peiwu Li; Yu Zhou. Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins. Food Chemistry 2020, 316, 126356 .
AMA StyleHualin Yang, Qi Zhang, Xiaolei Liu, Yuying Yang, Yong Yang, Mingyuan Liu, Peiwu Li, Yu Zhou. Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins. Food Chemistry. 2020; 316 ():126356.
Chicago/Turabian StyleHualin Yang; Qi Zhang; Xiaolei Liu; Yuying Yang; Yong Yang; Mingyuan Liu; Peiwu Li; Yu Zhou. 2020. "Antibody-biotin-streptavidin-horseradish peroxidase (HRP) sensor for rapid and ultra-sensitive detection of fumonisins." Food Chemistry 316, no. : 126356.
Background: Aflatoxin, which is highly carcinogenic and toxic, can seriously threaten the quality and safety of agricultural products, is mainly produced by Aspergillus flavus, which mainly exists in the soil of farmland. However, the correlation between aflatoxin, the microflora and environmental factors that survive in the roots of the plant is currently unknown. In this study, we used peanut-associated microbial populations as a model to address these issues.Results: We illustrated here that peanut pods significantly enriched with fungi and bacteria phyla. In the aflatoxin low pollution area, fungal and bacterial were more abundant than the high-pollution area, and the proportion of bacterial populations negatively correlated with aflatoxin was higher. However, some functions related to microbial-microbial and plant-microbial interactions were significantly enriched in areas with low levels of aflatoxin. Besides, we found that pH, Fe, Zn, P of the soil and temperature and humidity were the main factors that caused the differential composition and functional characteristics of microorganism. They can significantly affect the relationship between microbial flora and Aspergillus flavus and positively regulate aflatoxin production and microbial metabolism pathways, while OM, K, and other elements negatively regulated aflatoxin production and microbial metabolism pathways.Conclusions: The abundance of Aspergillus flavus and aflatoxin are significantly regulated by the population structure and function of microbiota, and this regulation is primarily affected by soil physical and chemical properties. Our results provide novel insights for understanding the contributions of the community enrichment process of the peanut pod-associated microbiome to the peanut hosts.
Yanpo Yao; Qi Zhang; Silivano Edson Mwakinyali; Suyan Gao; Xiaoxia Ding; Shuqing Liang; Zhongkui Xia; Peiwu Li. The peanut pods-associated microbiota and their effects on aflatoxin contamination. 2020, 1 .
AMA StyleYanpo Yao, Qi Zhang, Silivano Edson Mwakinyali, Suyan Gao, Xiaoxia Ding, Shuqing Liang, Zhongkui Xia, Peiwu Li. The peanut pods-associated microbiota and their effects on aflatoxin contamination. . 2020; ():1.
Chicago/Turabian StyleYanpo Yao; Qi Zhang; Silivano Edson Mwakinyali; Suyan Gao; Xiaoxia Ding; Shuqing Liang; Zhongkui Xia; Peiwu Li. 2020. "The peanut pods-associated microbiota and their effects on aflatoxin contamination." , no. : 1.
Diacetoxyscirpenol (DAS) is a type A trichothecene mycotoxin with low molecular weight, and with respect to its toxicity and the occurrence in food and feed, it is known as a potential risk for public and animal health. In the present study, first, a sensitive and specific monoclonal antibody (5E7) was developed. Then, the antibody was applied to develop a competitive-type pressure-dependent immunosensor (CTPDI). The [email protected] was synthesized and labeled with goat antimouse antibody ([email protected]). Finally, the concentration of DAS was negatively correlated with the pressure signal. In the presence of optimal conditions, matrix-matched calibration curves were plotted for wheat samples, in which an optimal IC50 value (half maximal inhibitory concentration) of 3.08 ng/g was achieved. The CTPDI was further applied to detect natural and blind wheat samples, and validation was carried out by liquid chromatography-tandem mass spectrometry. The results showed that CTPDI was highly appropriate and accurate for detection of DAS in wheat.
Xiaoqian Tang; Jing Wu; Wenqin Wu; Zhaowei Zhang; Weiqi Zhang; Qi Zhang; Wen Zhang; Xiaomei Chen; Peiwu Li. Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody. Analytical Chemistry 2020, 92, 3563 -3571.
AMA StyleXiaoqian Tang, Jing Wu, Wenqin Wu, Zhaowei Zhang, Weiqi Zhang, Qi Zhang, Wen Zhang, Xiaomei Chen, Peiwu Li. Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody. Analytical Chemistry. 2020; 92 (5):3563-3571.
Chicago/Turabian StyleXiaoqian Tang; Jing Wu; Wenqin Wu; Zhaowei Zhang; Weiqi Zhang; Qi Zhang; Wen Zhang; Xiaomei Chen; Peiwu Li. 2020. "Competitive-Type Pressure-Dependent Immunosensor for Highly Sensitive Detection of Diacetoxyscirpenol in Wheat via Monoclonal Antibody." Analytical Chemistry 92, no. 5: 3563-3571.
Aflatoxin contamination has been causing great concern worldwide due to the major economic impact on crop production and their toxicological effects to human and animals. Contamination can occur in the field, during transportation, and also in storage. Post-harvest contamination usually derives from the pre-harvest infection of aflatoxigenic molds, especially aflatoxin-producing Aspergilli such as Aspergillus flavus and A. parasiticus. Many strategies preventing aflatoxigenic molds from entering food and feed chains have been reported, among which biological control is becoming one of the most praised strategies. The objective of this article is to review the biocontrol strategy for inhibiting the growth of and aflatoxin production by aflatoxigenic fungi. This review focuses on comparing inhibitory behaviors of different antagonistic microorganisms including various bacteria, fungi and yeasts. We also reviewed the bioactive compounds produced by microorganisms and the mechanisms leading to inhibition. The key factors influencing antifungal activities of antagonists are also discussed in this review.
Xianfeng Ren; Qi Zhang; Wen Zhang; Jin Mao; Peiwu Li. Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors. Toxins 2020, 12, 24 .
AMA StyleXianfeng Ren, Qi Zhang, Wen Zhang, Jin Mao, Peiwu Li. Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors. Toxins. 2020; 12 (1):24.
Chicago/Turabian StyleXianfeng Ren; Qi Zhang; Wen Zhang; Jin Mao; Peiwu Li. 2020. "Control of Aflatoxigenic Molds by Antagonistic Microorganisms: Inhibitory Behaviors, Bioactive Compounds, Related Mechanisms, and Influencing Factors." Toxins 12, no. 1: 24.