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Chronic high-fat diet consumption induces hypercholesterolemia. The effect of Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) was studied on the levels of total cholesterol (TC), high-density lipoprotein cholesterol (HDL-c), and triglycerides, and on the expression of liver X receptors (LXRs) in a hypercholesterolemic model. Adult male rats received a normal diet (ND) or a high-fat diet (HFD; normal diet + bovine fat + cholesterol). After 14 days, rats received water (W) or a decoction of the aerial parts of T. absinthioides (Ta; 10% w/v) for 2, 4, or 6 weeks. Four and six weeks of Ta improved the levels of TC and HDL-c in HFD. After 6 weeks of Ta, the expression of LXRs in HFD was the same as that in ND in both tissues. The Ta chemical profile was studied with an ultrahigh resolution liquid chromatography Orbitrap MS analysis (UHPLC–PDA–OT-MS/MS). Fifty-one compounds were identified, of which twelve are reported for the first time. Among these compounds, caffeoylquinic acid and its derivatives could modify the lipid profile and the expression of LXRs. This is the first in vivo report of T. absinthioides, which may be a potential candidate against hypercholesterolemia.
Mariana Rey; María S. Kruse; Rocío N. Magrini-Huamán; Jessica Gómez; Mario J. Simirgiotis; Alejandro Tapia; Gabriela E. Feresin; Héctor Coirini. Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) Decoction Improves the Hypercholesterolemia and Alters the Expression of LXRs in Rat Liver and Hypothalamus. Metabolites 2021, 11, 579 .
AMA StyleMariana Rey, María S. Kruse, Rocío N. Magrini-Huamán, Jessica Gómez, Mario J. Simirgiotis, Alejandro Tapia, Gabriela E. Feresin, Héctor Coirini. Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) Decoction Improves the Hypercholesterolemia and Alters the Expression of LXRs in Rat Liver and Hypothalamus. Metabolites. 2021; 11 (9):579.
Chicago/Turabian StyleMariana Rey; María S. Kruse; Rocío N. Magrini-Huamán; Jessica Gómez; Mario J. Simirgiotis; Alejandro Tapia; Gabriela E. Feresin; Héctor Coirini. 2021. "Tessaria absinthioides (Hook. & Arn.) DC. (Asteraceae) Decoction Improves the Hypercholesterolemia and Alters the Expression of LXRs in Rat Liver and Hypothalamus." Metabolites 11, no. 9: 579.
Gaultheria pumila (Ericaceae) (known as Chaura or Mutilla) is a Chilean native small shrub that produces berry fruits consumed by local Mapuche people. In this study, the chemical fingerprinting and antioxidant, enzyme inhibition, and antiproliferative activities of the berries were investigated for the first time. Thirty-six metabolites were identified in the fruits by ultra-high performance liquid chromatography-photodiode array detection, hyphenated with Orbitrap mass spectrometry analysis (UHPLC-DAD-Orbitrap-MS). Metabolites, included anthocyanins, phenolic acids, flavonoids, iridoids, diterpenes, and fatty acids. Moderate inhibitory activities against acetylcholinesterase (7.7 ± 0.3 µg/mL), butyrylcholinesterase (34.5 ± 0.5 µg/mL), and tyrosinase (3.3 ± 0.2 µg/mL) enzymes were found. Moreover, selected major compounds were subjected to docking assays in light of their experimental inhibition. Results indicated that hydrogen bonding, π–π interaction, and a salt bridge interaction contributed significantly. Gaultheria pumila berries showed a total phenolic content of 189.2 ± 0.2 mg of gallic acid equivalents/g, total flavonoid content of 51.8 ± 0.1 mg quercetin equivalents/g, and total anthocyanin content of 47.3 ± 0.2 mg of cianydin-3-glucoside equivalents/g. Antioxidant activity was assessed using DPPH (92.8 ± 0.1 µg/mL), FRAP (134.1 ± 0.1 μmol Trolox equivalents/g), and ORAC (4251.6 ± 16.9 μmol Trolox equivalents/g) assays. Conversely, Gaultheria pumila showed a scarce antiproliferative potential against several solid human cancer cells. Our findings suggest that Gaultheria pumila berries have several bioactive metabolites with inhibitory effects against acetylcholinesterase, butyrylcholinesterase, and tyrosinase, and have the potential for use in food supplements.
Carlos Fernández-Galleguillos; Luisa Quesada-Romero; Adrián Puerta; José Padrón; Ernane Souza; Javier Romero-Parra; Mario Simirgiotis. UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries. Metabolites 2021, 11, 523 .
AMA StyleCarlos Fernández-Galleguillos, Luisa Quesada-Romero, Adrián Puerta, José Padrón, Ernane Souza, Javier Romero-Parra, Mario Simirgiotis. UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries. Metabolites. 2021; 11 (8):523.
Chicago/Turabian StyleCarlos Fernández-Galleguillos; Luisa Quesada-Romero; Adrián Puerta; José Padrón; Ernane Souza; Javier Romero-Parra; Mario Simirgiotis. 2021. "UHPLC-MS Chemical Fingerprinting and Antioxidant, Antiproliferative, and Enzyme Inhibition Potential of Gaultheria pumila Berries." Metabolites 11, no. 8: 523.
Haloarchaea are extreme halophilic microorganisms belonging to the domain Archaea, phylum Euryarchaeota, and are producers of interesting antioxidant carotenoid compounds. In this study, four new strains of Haloarcula sp., isolated from saline lakes of the Atacama Desert, are reported and studied by high-resolution mass spectrometry (UHPLC-Q-Orbitrap-MS/MS) for the first time. In addition, determination of the carotenoid pigment profile from the new strains of Haloarcula sp., plus two strains of Halorubrum tebenquichense, and their antioxidant activity by means of several methods is reported. The effect of biomass on cellular viability in skin cell lines was also evaluated by MTT assay. The cholinesterase inhibition capacity of six haloarchaea (Haloarcula sp. ALT-23; Haloarcula sp. TeSe-41; Haloarcula sp. TeSe-51; Haloarcula sp. Te Se-89 and Halorubrum tebenquichense strains TeSe-85 and Te Se-86) is also reported for the first time. AChE inhibition IC50 was 2.96 ± 0.08 μg/mL and BuChE inhibition IC50 was 2.39 ± 0.09 μg/mL for the most active strain, Halorubrum tebenquichense Te Se-85, respectively, which is more active in BuCHe than that of the standard galantamine. Docking calculation showed that carotenoids can exert their inhibitory activity fitting into the enzyme pocket by their halves, in the presence of cholinesterase dimers.
Catherine Lizama; Javier Romero-Parra; Daniel Andrade; Felipe Riveros; Jorge Bórquez; Shakeel Ahmed; Luis Venegas-Salas; Carolina Cabalín; Mario Simirgiotis. Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability. Antioxidants 2021, 10, 1230 .
AMA StyleCatherine Lizama, Javier Romero-Parra, Daniel Andrade, Felipe Riveros, Jorge Bórquez, Shakeel Ahmed, Luis Venegas-Salas, Carolina Cabalín, Mario Simirgiotis. Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability. Antioxidants. 2021; 10 (8):1230.
Chicago/Turabian StyleCatherine Lizama; Javier Romero-Parra; Daniel Andrade; Felipe Riveros; Jorge Bórquez; Shakeel Ahmed; Luis Venegas-Salas; Carolina Cabalín; Mario Simirgiotis. 2021. "Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability." Antioxidants 10, no. 8: 1230.
In the present work, we report the isolation of five alkaloids from the seeds of Erythrina rubrinervia. Four of the isolated alkaloids are erythrinoid type alkaloids which were identified as erysodine (1), erysovine (2), erythraline (3) and erysotrine (4), plus an indolic alkaloid which was identified as hypaphorine (5). The analysis of spectroscopic data for the alkaloid l-hypaphorine shows that the published structure (5a) must be revised, and the correct structure is that depicted as the structure 5c. The chemical structures were elucidated by full spectroscopic analysis. The chemotaxonomic significance of those findings in the genus Erythrina is also discussed.
Simón Urrea Florian; Beatriz Sepulveda; Alfredo Torres-Benítez; Mario J. Simirgiotis; Marcial Fuentes-Estrada; Carlos Areche; Olimpo García-Beltrán. Erythrinoid and indol alkaloids isolated from the seeds of Erythrina rubrinervia Kunth: Chemotaxonomic significance. Biochemical Systematics and Ecology 2021, 97, 104295 .
AMA StyleSimón Urrea Florian, Beatriz Sepulveda, Alfredo Torres-Benítez, Mario J. Simirgiotis, Marcial Fuentes-Estrada, Carlos Areche, Olimpo García-Beltrán. Erythrinoid and indol alkaloids isolated from the seeds of Erythrina rubrinervia Kunth: Chemotaxonomic significance. Biochemical Systematics and Ecology. 2021; 97 ():104295.
Chicago/Turabian StyleSimón Urrea Florian; Beatriz Sepulveda; Alfredo Torres-Benítez; Mario J. Simirgiotis; Marcial Fuentes-Estrada; Carlos Areche; Olimpo García-Beltrán. 2021. "Erythrinoid and indol alkaloids isolated from the seeds of Erythrina rubrinervia Kunth: Chemotaxonomic significance." Biochemical Systematics and Ecology 97, no. : 104295.
Global fisheries production has increased up to ~200 MT, which has resulted in the intensive generation of waste or byproducts (~20 MT), which is creating serious problems for environmental management with zero income. This study proposes an idea of using whole fish (red and white meat, skin, bones, and fins but not scales) for human food (snack food) with the aim of zero waste generation. Whole fish powder (WFP) was prepared by a novel method (using freeze-drying as well as stone ball milling) and fortified in baked snacks at four levels (0, 5, 10, and 15% w/w of 100 g of formulation). The results revealed that the addition of WFP decreased expansion and color parameters compared to control. Hardness was significantly (p< 0.05) increased with the addition of WFP, which was attributed to the mineral content of WFP. Pasting properties determined by rapid visco analyzer (RVA) were dramatically decreased with the addition of 10–15% WFP, suggesting the weak interaction of starch and protein, which was also evidenced by scanning electron microscopy (SEM). Low field nuclear magnetic resonance (LF-NMR) analysis revealed that the amount of free water was increased when 10–15% WFP was added in snacks while bound water was highest in control and 5% WFP samples, respectively. The microstructural analysis by SEM showed that the protein network was increased in those samples incorporated with WFP compared to control that had more starch granules. The results suggest the feasibility of adding 5% WFP for proper structure, texture, pasting properties, and water distribution in order to reduce fish waste.
Asad Nawaz; Ibrahim Khalifa; Noman Walayat; Jose Lorenzo; Sana Irshad; Abdullah; Shakeel Ahmed; Mario Simirgiotis; Madad Ali; Enpeng Li. Whole Fish Powder Snacks: Evaluation of Structural, Textural, Pasting, and Water Distribution Properties. Sustainability 2021, 13, 6010 .
AMA StyleAsad Nawaz, Ibrahim Khalifa, Noman Walayat, Jose Lorenzo, Sana Irshad, Abdullah, Shakeel Ahmed, Mario Simirgiotis, Madad Ali, Enpeng Li. Whole Fish Powder Snacks: Evaluation of Structural, Textural, Pasting, and Water Distribution Properties. Sustainability. 2021; 13 (11):6010.
Chicago/Turabian StyleAsad Nawaz; Ibrahim Khalifa; Noman Walayat; Jose Lorenzo; Sana Irshad; Abdullah; Shakeel Ahmed; Mario Simirgiotis; Madad Ali; Enpeng Li. 2021. "Whole Fish Powder Snacks: Evaluation of Structural, Textural, Pasting, and Water Distribution Properties." Sustainability 13, no. 11: 6010.
Cocoa beans contain antioxidant molecules with the potential to inhibit type 2 coronavirus (SARS-CoV-2), which causes a severe acute respiratory syndrome (COVID-19). In particular, protease. Therefore, using in silico tests, 30 molecules obtained from cocoa were evaluated. Using molecular docking and quantum mechanics calculations, the chemical properties and binding efficiency of each ligand was evaluated, which allowed the selection of 5 compounds of this series. The ability of amentoflavone, isorhoifolin, nicotiflorin, naringin and rutin to bind to the main viral protease was studied by means of free energy calculations and structural analysis performed from molecular dynamics simulations of the enzyme/inhibitor complex. Isorhoifolin and rutin stand out, presenting a more negative binding ΔG than the reference inhibitor N-[(5-methylisoxazol-3-yl)carbonyl]alanyl-l-valyl-N~1~-((1R,2Z)−4-(benzyloxy)−4-oxo-1-{[(3R)−2-oxopyrrolidin-3-yl]methyl}but-2-enyl)-L-leucinamide (N3). These results are consistent with high affinities of these molecules for the major SARS-CoV-2. The results presented in this paper are a solid starting point for future in vitro and in vivo experiments aiming to validate these molecules and /or test similar substances as inhibitors of SARS-CoV-2 protease.
Osvaldo Yañez; Manuel Isaías Osorio; Carlos Areche; Alejandro Vasquez-Espinal; Jessica Bravo; Angélica Sandoval-Aldana; José M. Pérez-Donoso; Fernando González-Nilo; Maria João Matos; Edison Osorio; Olimpo García-Beltrán; William Tiznado. Theobroma cacao L. compounds: Theoretical study and molecular modeling as inhibitors of main SARS-CoV-2 protease. Biomedicine & Pharmacotherapy 2021, 140, 111764 -111764.
AMA StyleOsvaldo Yañez, Manuel Isaías Osorio, Carlos Areche, Alejandro Vasquez-Espinal, Jessica Bravo, Angélica Sandoval-Aldana, José M. Pérez-Donoso, Fernando González-Nilo, Maria João Matos, Edison Osorio, Olimpo García-Beltrán, William Tiznado. Theobroma cacao L. compounds: Theoretical study and molecular modeling as inhibitors of main SARS-CoV-2 protease. Biomedicine & Pharmacotherapy. 2021; 140 ():111764-111764.
Chicago/Turabian StyleOsvaldo Yañez; Manuel Isaías Osorio; Carlos Areche; Alejandro Vasquez-Espinal; Jessica Bravo; Angélica Sandoval-Aldana; José M. Pérez-Donoso; Fernando González-Nilo; Maria João Matos; Edison Osorio; Olimpo García-Beltrán; William Tiznado. 2021. "Theobroma cacao L. compounds: Theoretical study and molecular modeling as inhibitors of main SARS-CoV-2 protease." Biomedicine & Pharmacotherapy 140, no. : 111764-111764.
The Lamiaceae family is an important source of species among medicinal plants highly valued for their biological properties and numerous uses in folk medicine. Origanum is one of the main genera that belong to this family. The purpose of the study was to determine the phenolic composition of the Origanum vulgare extract and evaluate the antimicrobial, antioxidant, and inhibitory activities of this species that grows in the Andean region of the Atacama Desert. High-performance liquid chromatography was performed to determine the main phenols. Rosmarinic acid was identified as the predominant phenolic compound in this species (76.01 mg/100 g DW), followed by protocatechuic acid, which to our knowledge, no previous study reported similar concentrations in O. vulgare. The oregano extract exhibited a content of total phenolic (3948 mg GAE/100 g DW) and total flavonoid (593 mg QE/100 g DW) with a higher DPPH antioxidant activity (IC50 = 40.58 µg/mL), compared to the same species grown under other conditions. Furthermore, it was found to inhibit α-glucosidase activity with an IC50 value (7.11 mg/mL) lower than acarbose (129.32 mg/mL). Pseudomonas syringae and Pantoea agglomerans (both MIC 0.313 mg/mL and MBC 1.25 mg/mL) were the bacteria most susceptible to oregano extract with the lowest concentration necessary to inhibit bacterial growth. These results open the door for the potential use of this plant to manage chronic diseases, and they expand the knowledge of the species cultivated in arid environmental conditions.
Claudio Parra; Patricio Muñoz; Luis Bustos; Felipe Parra; Mario Simirgiotis; Hugo Escobar. UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities. Molecules 2021, 26, 2100 .
AMA StyleClaudio Parra, Patricio Muñoz, Luis Bustos, Felipe Parra, Mario Simirgiotis, Hugo Escobar. UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities. Molecules. 2021; 26 (7):2100.
Chicago/Turabian StyleClaudio Parra; Patricio Muñoz; Luis Bustos; Felipe Parra; Mario Simirgiotis; Hugo Escobar. 2021. "UHPLC-DAD Characterization of Origanum vulgare L. from Atacama Desert Andean Region and Antioxidant, Antibacterial and Enzyme Inhibition Activities." Molecules 26, no. 7: 2100.
This research was designed to investigate the metabolite profiling, phenolics and flavonoids content and the potential antioxidant, antibacterial and nematicidal activities of “yellow-brown resins” from Larrea divaricata Cav (LdRe) and L. nitida Cav (LnRe). Metabolite profiling was obtained using an ultrahigh resolution liquid chromatography orbitrap MS analysis (UHPLC-ESI-OT-MS). The antioxidant properties were screened by four methods: 2,2-diphenyl-1-picrylhydrazyl assay (DPPH), trolox equivalent antioxidant activity assay (TEAC), ferric-reducing antioxidant power assay (FRAP) and lipid peroxidation in erythrocytes (LP). The antibacterial activity was evaluated according to the Clinical and Laboratory Standards Institute (CLSI) guidelines. In addition, the potential combinatory effect was analyzed with the fractional inhibitory concentration index (FICI) values using the checkerboard design. The nematicidal activity was carried out according to a standardized protocol. LdRe and LnRe showed a strong capture of the DPPH radical withvalues around 8.4 µg resin/mL; FRAP (1.69–1.94 mgTE/ g resin), TEAC (1.08–1.09 mgTE/g resin) and LP (81–82% at 100 µg of resin/mL) assays. A strong antimicrobial activity was displayed by both resins against methicillin-sensitive Staphylococcus aureus ATCC 25923(MSSA) and methicillin-resistant S. aureus ATCC 43300(MRSA) (MICs = 16–32 µg resin/mL). Additionally, the combination of LdRe or LnRe with the antibiotic cefotaxime showed an indifferent effect (FICI values = 1–1.25), however, this combinationcould be a potential strategy to reduce the drug doses, and in this way can be a potential alternative to reduce bacterial resistance. On the other hand, the resins showed a scarce nematicidal potential toward J2 Meloidogyne incognita; an important nematode infecting horticultural crops. Phenolics compounds were identified by UHPLC-PDA-OT-MS analysis, updating the knowledge on the chemical profile of these species. These results, together with the high content of quantified phenolics and flavonoids, allow the phenolics-enriched resins of these two Larrea species to be considered as a promising sustainable source of compounds of pharmacological interest.
Jessica Gómez; Mario Simirgiotis; Sofía Manrique; Mauricio Piñeiro; Beatriz Lima; Jorge Bórquez; Gabriela Feresin; Alejandro Tapia. UHPLC-ESI-OT-MS Phenolics Profiling, Free Radical Scavenging, Antibacterial and Nematicidal Activities of “Yellow-Brown Resins” from Larrea spp. Antioxidants 2021, 10, 185 .
AMA StyleJessica Gómez, Mario Simirgiotis, Sofía Manrique, Mauricio Piñeiro, Beatriz Lima, Jorge Bórquez, Gabriela Feresin, Alejandro Tapia. UHPLC-ESI-OT-MS Phenolics Profiling, Free Radical Scavenging, Antibacterial and Nematicidal Activities of “Yellow-Brown Resins” from Larrea spp. Antioxidants. 2021; 10 (2):185.
Chicago/Turabian StyleJessica Gómez; Mario Simirgiotis; Sofía Manrique; Mauricio Piñeiro; Beatriz Lima; Jorge Bórquez; Gabriela Feresin; Alejandro Tapia. 2021. "UHPLC-ESI-OT-MS Phenolics Profiling, Free Radical Scavenging, Antibacterial and Nematicidal Activities of “Yellow-Brown Resins” from Larrea spp." Antioxidants 10, no. 2: 185.
The aim of this review is to provide overall information on Argentine propolis and to shed light on its potential, especially the one from the Monte region so as to support future research in the field. Around 1999, the Argentine propolis began to be chemically and functionally characterized to give it greater added value. Because Argentina has a wide plant biodiversity, it is expected that its propolis will have various botanical origins, and consequently, a different chemical composition. To date, five types have been defined. Based on their functionality, several products have been developed for use in human and veterinary medicine and in animal and human food. Because the Argentine propolis with the greatest potential is that of the Monte eco-region, this review will describe the findings of the last 20 years on this propolis, its botanical source (Zuccagnia punctata Cav.), its chemical composition, and a description of markers of chemical quality (chalcones) and functionality. Propolis can regulate the activity of various pro-inflammatory enzymes and carbohydrate and lipid metabolism enzymes, as well as remove reactive oxygen and nitrogen species. Consequently, it can modulate metabolic syndrome and could be used as a functional ingredient in food. Furthermore, hydroalcoholic extracts can act against human and animal pathogenic bacteria and human yeast, and mycelial pathogenic fungi. The ability to stop the growth of post-harvest pathogenic bacteria and fungi was also demonstrated. For this reason, Argentine propolis are natural products capable of protecting crops and increasing the lifespan of harvested fruit and vegetables. Several reports indicate the potential of Argentine propolis to be used in innovative products to improve health, food preservation, and packaging. However, there is still much to learn about these natural products to make a wholesome use of them.
Iris Zampini; Ana Salas; Luis Maldonado; Mario Simirgiotis; María Isla. Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient. Metabolites 2021, 11, 76 .
AMA StyleIris Zampini, Ana Salas, Luis Maldonado, Mario Simirgiotis, María Isla. Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient. Metabolites. 2021; 11 (2):76.
Chicago/Turabian StyleIris Zampini; Ana Salas; Luis Maldonado; Mario Simirgiotis; María Isla. 2021. "Propolis from the Monte Region in Argentina: A Potential Phytotherapic and Food Functional Ingredient." Metabolites 11, no. 2: 76.
Mitochondria is a cellular source of energy, appears to play an essential role in dealing with cellular stress induced by environmental stimuli. The genetic diversity of mitochondrial genes involved in oxidative phosphorylation affecting the production of cellular energy and regional adaptation to various ecological (climatic) pressures affecting amino acid sequences (variants of protein). However, little is known about the combined effect of protein changes on cell-level metabolic alterations in simultaneous exposure to various environmental conditions, including mitochondrial dysfunction and oxidative stress induction. The present study was designed to address this issue by analyzing the mitochondrial proteins in Fasciola species including Cytochrome oxidase (COX1, COX2, COX3, and CYTB) and NADH dehydrogenase (ND1, ND2, ND3, ND4, ND5, and ND6). Mitochondrial proteins were used for detailed computational investigation, using available standard bioinformatics tools to exploit structural and functional relationships. These proteins in Fasciola hepatica, Fasciola gigentica, and Fasciola jacksoni were functionally annotated using public databases. The results showed that the protein of COX1 of F. hepatica, F. gigantica, and F. jacksoni consist of 510, 513, and 517 amino acids, respectively. The alignment of proteins showed that these proteins are conserved in the same regions at ten positions in COX and CYTB proteins while at twelve locations in NADH. Three-dimensional structure of COX, CYTB, and NADH proteins were compared and showed differences in additional conserved and binding sites in COX and CYTB proteins as compared to NADH in three species of Fasciola. These results based on the amino acid diversity pattern were used to identify sites in the enzyme and the variations in mitochondrial proteins among Fasciola species. Our study provides valuable information for future experimental studies, including identification of therapeutic, diagnostic, and immunoprophylactic interests with novel mitochondrial proteins.
Hafiz Ishfaq Ahmad; Muhammad Bilal Bin Majeed; Muhammad Zulfiqar Ahmad; Abdul Jabbar; Babar Maqbool; Shakeel Ahmed; Hamid Mustafa; Mario Juan Simirgiotis; Jinping Chen. Comparative analysis of the mitochondrial proteins reveals complex structural and functional relationships in Fasciola species. Microbial Pathogenesis 2021, 152, 104754 .
AMA StyleHafiz Ishfaq Ahmad, Muhammad Bilal Bin Majeed, Muhammad Zulfiqar Ahmad, Abdul Jabbar, Babar Maqbool, Shakeel Ahmed, Hamid Mustafa, Mario Juan Simirgiotis, Jinping Chen. Comparative analysis of the mitochondrial proteins reveals complex structural and functional relationships in Fasciola species. Microbial Pathogenesis. 2021; 152 ():104754.
Chicago/Turabian StyleHafiz Ishfaq Ahmad; Muhammad Bilal Bin Majeed; Muhammad Zulfiqar Ahmad; Abdul Jabbar; Babar Maqbool; Shakeel Ahmed; Hamid Mustafa; Mario Juan Simirgiotis; Jinping Chen. 2021. "Comparative analysis of the mitochondrial proteins reveals complex structural and functional relationships in Fasciola species." Microbial Pathogenesis 152, no. : 104754.
Cancer is still one of the leading causes of death worldwide. Many chemotherapeutics from plants have been tested in cancer, such as vinblastine and paclitaxel. The north of Chile, Arica & Parinacota region, is characterized by its vegetal biodiversity due to its unique geographical and climatological conditions, offering an unexplored and unique source of naturally-derived compounds. The present research has considered a screening of 26 highland herbs using an in vitro growth inhibition model in a panel of six cancer cell lines from different tissues. 5 of the 26 studied ethanolic extracts shows strong activity at least in one cell line when tested at 10 µg mL-1. We present a group of plants worthy to be evaluated as promissory extracts. This work is part of the systematic attempt to find new candidates to be used in cancer therapies.
Luis Bustos González; Mario Juan Simirgiotis; Claudio Parra; Susana Alfaro-Lira; Emilio Soto; Carlos Echiburú-Chau. In vitro screening for growth inhibition activity on cancer cell lines of northern Chile highlands shrubs. Ciência Rural 2021, 51, 1 .
AMA StyleLuis Bustos González, Mario Juan Simirgiotis, Claudio Parra, Susana Alfaro-Lira, Emilio Soto, Carlos Echiburú-Chau. In vitro screening for growth inhibition activity on cancer cell lines of northern Chile highlands shrubs. Ciência Rural. 2021; 51 (1):1.
Chicago/Turabian StyleLuis Bustos González; Mario Juan Simirgiotis; Claudio Parra; Susana Alfaro-Lira; Emilio Soto; Carlos Echiburú-Chau. 2021. "In vitro screening for growth inhibition activity on cancer cell lines of northern Chile highlands shrubs." Ciência Rural 51, no. 1: 1.
Parkinson's disease (PD) is a neurodegenerative disorder that affects adult people whose treatment is palliative. Thus, we decided to test three dammarane triterpenes 1, 1a, 1b, and we determined that 1 and 1a inhibit β-aggregation through thioflavine T rather than 1b. Since compound 1 was most active, we determined the interaction between α-synuclein and 1 at 50 µM (Kd) through microscale thermophoresis. Also, we observed differences in height and diameter of aggregates, and α-synuclein remains unfolded in the presence of 1. Also, aggregates treated with 1 do not provoke neurites' retraction in N2a cells previously induced by retinoic acid. Finally, we studied the potential sites of interaction between 1 with α-synuclein fibrils using molecular modelling. Docking experiments suggest that 1 preferably interact with the site 2 of α-synuclein through hydrogen bonds with residues Y39 and T44. Graphical Abstract
Alberto Cornejo; Julio Caballero; Mario Simirgiotis; Vanessa Torres; Luisa Sánchez; Nicolás Díaz; Marcela Guimaraes; Marcos Hernández; Carlos Areche; Sergio Alfaro; Leonardo Caballero; Francisco Melo. Dammarane triterpenes targeting α-synuclein: biological activity and evaluation of binding sites by molecular docking. Journal of Enzyme Inhibition and Medicinal Chemistry 2020, 36, 154 -162.
AMA StyleAlberto Cornejo, Julio Caballero, Mario Simirgiotis, Vanessa Torres, Luisa Sánchez, Nicolás Díaz, Marcela Guimaraes, Marcos Hernández, Carlos Areche, Sergio Alfaro, Leonardo Caballero, Francisco Melo. Dammarane triterpenes targeting α-synuclein: biological activity and evaluation of binding sites by molecular docking. Journal of Enzyme Inhibition and Medicinal Chemistry. 2020; 36 (1):154-162.
Chicago/Turabian StyleAlberto Cornejo; Julio Caballero; Mario Simirgiotis; Vanessa Torres; Luisa Sánchez; Nicolás Díaz; Marcela Guimaraes; Marcos Hernández; Carlos Areche; Sergio Alfaro; Leonardo Caballero; Francisco Melo. 2020. "Dammarane triterpenes targeting α-synuclein: biological activity and evaluation of binding sites by molecular docking." Journal of Enzyme Inhibition and Medicinal Chemistry 36, no. 1: 154-162.
Artemisia copa Phil. (Asteraceae) (known as copa-copa) is a native species of Chile used as an infusion in traditional medicine by Atacameños people in the Altiplano, highlands of northern Chile. In this research, we have investigated for the first time the cholinesterase inhibition potential against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE), and the chemical profiling of the infusions prepared from the aerial parts of A. copa by high resolution spectrometry. In addition, total phenolic, total flavonoid content, antioxidant (DPPH, FRAP, and ORAC) and antiprozoal activity were tested. Artemisia copa showed good inhibitory activity against AChE and BChE (3.92 ± 0.08 µg/ml and 44.13 ± 0.10 µg/ml). The infusion displayed a total phenolics content of 155.6 ± 2.9 mg of gallic acid equivalents/g and total flavonoid content of 5.5 ± 0.2 mg quercetin equivalents/g. Additionally, trypanocidal activity against Trypanosoma cruzi was found (LD50 of 131.8 µg/ml). Forty-seven metabolites were detected in the infusion of A. copa including several phenolic acids and flavonoids which were rapidly identified using ultrahigh performance liquid chromatography orbitrap mass spectrometry analysis (UHPLC-Orbitrap-MS) for chemical profiling. The major compounds identified in the infusions were studied by molecular docking against AChE and BChE. The UHPLC-MS fingerprints generated can be also used for the authentication of these endemic species. These findings reveal that A. copa infusions can be used as beverages with protective effects.
María José Larrazábal-Fuentes; Carlos Fernández-Galleguillos; Jenifer Palma-Ramírez; Javier Romero-Parra; Kevin Sepúlveda; Alexandra Galetovic; Jorge González; Adrián Paredes; Jorge Bórquez; Mario J. Simirgiotis; Javier Echeverría. Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae). Frontiers in Pharmacology 2020, 11, 1 .
AMA StyleMaría José Larrazábal-Fuentes, Carlos Fernández-Galleguillos, Jenifer Palma-Ramírez, Javier Romero-Parra, Kevin Sepúlveda, Alexandra Galetovic, Jorge González, Adrián Paredes, Jorge Bórquez, Mario J. Simirgiotis, Javier Echeverría. Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae). Frontiers in Pharmacology. 2020; 11 ():1.
Chicago/Turabian StyleMaría José Larrazábal-Fuentes; Carlos Fernández-Galleguillos; Jenifer Palma-Ramírez; Javier Romero-Parra; Kevin Sepúlveda; Alexandra Galetovic; Jorge González; Adrián Paredes; Jorge Bórquez; Mario J. Simirgiotis; Javier Echeverría. 2020. "Chemical Profiling, Antioxidant, Anticholinesterase, and Antiprotozoal Potentials of Artemisia copa Phil. (Asteraceae)." Frontiers in Pharmacology 11, no. : 1.
The genus Blechnum L. includes a large variety of species native to central and southern continental Chile: B. arcuatum, B. asperum, B. blechnoides, B. chilense, B. corralense, B hastatum, B. magellanicum, B. microphyllum, B. mochaenum and B. penna-marina, containing compounds such as flavonols, hydroxycinnamic acids, lignans, and phytoecdysteroids. In this examination, terpenic compounds of the phytoecdysone type with pharmacological potential on the central nervous system are detailed. These compounds are characterized by a C27, C28 or C29 structure that possesses a 14-alpha-hydroxy-7-en-6-one chromophore and an A/B-cis (5beta-H) ring fusion that can be extracted from the plant with organic solvents, such as hexane, dichloromethane, ethyl acetate, methanol, and ethanol, is concentrated in a reduced pressure rotary evaporator and are identified by gas chromatography coupled to a mass spectrometry detector (GC-MS) or a tandem ionization electrospray network detector UHPLC-DAD-ESI-MS/MS. Phytoecdysteroids applied in vitro and in vivo models of neurodegenerative diseases such as Parkinson’s and Alzheimer’s at concentrations between 100 and 500 µg/mL, can modulate the enzymatic inhibition of monoamine oxidases (MAO-A and MAO-B) and cholinesterases (AChE and BChE), with zero toxicity effects and satisfactory results in cell viability.
Mathias Flores-González; Alfredo Torres-Benítez; Mario Simirgiotis. Terpenic Compounds in Chilean Species of the Genus Blechnum (Pteridophyta: Blechnaceae) with Neuroprotective Potential. Proceedings 2020, 71, 2 .
AMA StyleMathias Flores-González, Alfredo Torres-Benítez, Mario Simirgiotis. Terpenic Compounds in Chilean Species of the Genus Blechnum (Pteridophyta: Blechnaceae) with Neuroprotective Potential. Proceedings. 2020; 71 (1):2.
Chicago/Turabian StyleMathias Flores-González; Alfredo Torres-Benítez; Mario Simirgiotis. 2020. "Terpenic Compounds in Chilean Species of the Genus Blechnum (Pteridophyta: Blechnaceae) with Neuroprotective Potential." Proceedings 71, no. 1: 2.
Mangifera indica Linn popularly known as mango is used in folk medicine to treat gastrointestinal disorders. The aim of this study was to identify the metabolomic composition of lyophilized extract of mango leaf (MIE), to evaluate the antioxidant activity on several oxidative stress systems (DPPH, FRAP, TBARS, and ABTS), the spasmolytic and antispasmodic activity, and intestinal protective effect on oxidative stress induced by H2O2 in rat ileum. Twenty-nine metabolites were identified and characterized based on their ultra-high-performance liquid chromatography (UHPLC) high-resolution orbitrap mass spectrometry, these include: benzophenone derivatives, xanthones, phenolic acids, fatty acids, flavonoids and procyanidins. Extract demonstrated a high antioxidant activity in in-vitro assays. MIE relaxed (p < 0.001) intestinal segments of rat pre-contracted with acetylcholine (ACh) (10−5 M). Pre-incubation of intestinal segments with 100 µg/mL MIE significantly reduced (p < 0.001) the contraction to H2O2. Similar effects were observed with mangiferin and quercetin (10−5 M; p < 0.05) but not for gallic acid. Chronic treatment of rats with MIE (50 mg/kg) for 28 days significantly reduced (p < 0.001) the H2O2-induced contractions. MIE exhibited a strong antioxidant activity, spasmolytic and antispasmodic activity, which could contribute to its use as an alternative for the management of several intestinal diseases related to oxidative stress.
Roberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules 2020, 25, 5149 .
AMA StyleRoberto Ybañez-Julca, Daniel Asunción-Alvarez, Ivan Quispe-Díaz, Javier Palacios, Jorge Bórquez, Mario Simirgiotis, Shagufta Perveen, Chukwuemeka Nwokocha, Fredi Cifuentes, Adrián Paredes. Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models. Molecules. 2020; 25 (21):5149.
Chicago/Turabian StyleRoberto Ybañez-Julca; Daniel Asunción-Alvarez; Ivan Quispe-Díaz; Javier Palacios; Jorge Bórquez; Mario Simirgiotis; Shagufta Perveen; Chukwuemeka Nwokocha; Fredi Cifuentes; Adrián Paredes. 2020. "Metabolomic Profiling of Mango (Mangifera indica Linn) Leaf Extract and Its Intestinal Protective Effect and Antioxidant Activity in Different Biological Models." Molecules 25, no. 21: 5149.
This study aimed to characterize the in vitro antioxidant and antibacterial properties of oregano (Origanum vulgare) essential oil, as well as its chemical composition. To our best knowledge, there are few studies on oregano grown in the arid Andes region, but none on the metabolites produced and their bioactivity. This work identified fifty metabolites by Gas Chromatography–Mass Spectrometry (GC-MS)—monoterpene hydrocarbons, oxygenated monoterpenes, phenolic monoterpenes, sesquiterpene hydrocarbons, and oxygenated sesquiterpenes—present in the essential oil of oregano collected in the Atacama Desert. The main components of essential oregano oil were thymol (15.9%), Z-sabinene hydrate (13.4%), γ-terpinene (10.6%), p-cymene (8.6%), linalyl acetate (7.2%), sabinene (6.5%), and carvacrol methyl ether (5.6%). The antibacterial tests showed that the pathogenic bacteria Staphylococcus aureus and Salmonella enterica and the phytopathogenic bacteria Erwinia rhapontici and Xanthomonas campestris were the most susceptible to oregano oil, with the lowest concentrations of oil necessary to inhibit their bacterial growth. Moreover, oregano oil showed antibacterial activity against bacteria associated with food poisoning. In conclusion, O. vulgare from the arid Andean region possesses an important antibacterial activity with a high potential in the food industry and agriculture.
Mario J. Simirgiotis; Daniel Burton; Felipe Parra; Jéssica López; Patricio Muñoz; Hugo Escobar; Claudio Parra. Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS. Metabolites 2020, 10, 414 .
AMA StyleMario J. Simirgiotis, Daniel Burton, Felipe Parra, Jéssica López, Patricio Muñoz, Hugo Escobar, Claudio Parra. Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS. Metabolites. 2020; 10 (10):414.
Chicago/Turabian StyleMario J. Simirgiotis; Daniel Burton; Felipe Parra; Jéssica López; Patricio Muñoz; Hugo Escobar; Claudio Parra. 2020. "Antioxidant and Antibacterial Capacities of Origanum vulgare L. Essential Oil from the Arid Andean Region of Chile and its Chemical Characterization by GC-MS." Metabolites 10, no. 10: 414.
Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) is a Chilean endemic plant popularly known as “quiscal” and produces an edible fruit consumed by the local Mapuche communities named as “chupón”. In this study, several metabolites including phenolic acids, organic acids, sugar derivatives, catechins, proanthocyanidins, fatty acids, iridoids, coumarins, benzophenone, flavonoids, and terpenes were identified in G. sphacelata fruits using ultrahigh performance liquid chromatography-photodiode array detection coupled with a Orbitrap mass spectrometry (UHPLC-PDA-Orbitrap-MS) analysis for the first time. The fruits showed moderate antioxidant capacities (i.e., 487.11 ± 26.22 μmol TE/g dry weight) in the stable radical DPPH assay, 169.08 ± 9.81 TE/g dry weight in the ferric reducing power assay, 190.32 ± 6.23 TE/g dry weight in the ABTS assay, and 76.46 ± 3.18% inhibition in the superoxide anion scavenging assay. The cholinesterase inhibitory potential was evaluated against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). From the findings, promising results were observed for pulp and seeds. Our findings suggest that G. sphacelata fruits are a rich source of diverse secondary metabolites with antioxidant capacities. In addition, the inhibitory effects against AChE and BChE suggest that natural products or food supplements derived from G. sphacelata fruits are of interest for their neuroprotective potential.
Ruth E. Barrientos; Shakeel Ahmed; Carmen Cortés; Carlos Fernández-Galleguillos; Javier Romero-Parra; Mario J. Simirgiotis; Javier Echeverría. Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry. Molecules 2020, 25, 3750 .
AMA StyleRuth E. Barrientos, Shakeel Ahmed, Carmen Cortés, Carlos Fernández-Galleguillos, Javier Romero-Parra, Mario J. Simirgiotis, Javier Echeverría. Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry. Molecules. 2020; 25 (16):3750.
Chicago/Turabian StyleRuth E. Barrientos; Shakeel Ahmed; Carmen Cortés; Carlos Fernández-Galleguillos; Javier Romero-Parra; Mario J. Simirgiotis; Javier Echeverría. 2020. "Chemical Fingerprinting and Biological Evaluation of the Endemic Chilean Fruit Greigia sphacelata (Ruiz and Pav.) Regel (Bromeliaceae) by UHPLC-PDA-Orbitrap-Mass Spectrometry." Molecules 25, no. 16: 3750.
Heliotropium taltalense is an endemic species of the northern coast of Chile and is used as folk medicine. The polyphenolic composition of the methanolic and aqueous extract of the endemic Chilean species was investigated using Ultrahigh-Performance Liquid Chromatography, Heated Electrospray Ionization and Mass Spectrometry (UHPLC-Orbitrap-HESI-MS). Fifty-three compounds were detected, mainly derivatives of benzoic acid, flavonoids, and some phenolic acids. Furthermore, five major compounds were isolated by column chromatography from the extract, including four flavonoids and one geranyl benzoic acid derivative, which showed vascular relaxation and were in part responsible for the activity of the extracts. Since aqueous extract of H. taltalense (83% ± 9%, 100 μg/mL) produced vascular relaxation through an endothelium-dependent mechanism in rat aorta, and the compounds rhamnocitrin (89% ± 7%; 10−4 M) and sakuranetin (80% ± 6%; 10−4 M) also caused vascular relaxation similar to the extracts of H. taltalense, these pure compounds are, to some extent, responsible for the vascular relaxation.
Ruth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules 2020, 25, 3105 .
AMA StyleRuth E. Barrientos, Mario J. Simirgiotis, Javier Palacios, Adrián Paredes, Jorge Bórquez, Alejandra Bravo, Fredi Cifuentes. Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta. Molecules. 2020; 25 (14):3105.
Chicago/Turabian StyleRuth E. Barrientos; Mario J. Simirgiotis; Javier Palacios; Adrián Paredes; Jorge Bórquez; Alejandra Bravo; Fredi Cifuentes. 2020. "Chemical Fingerprinting, Isolation and Characterization of Polyphenol Compounds from Heliotropium taltalense (Phil.) I.M. Johnst and Its Endothelium-Dependent Vascular Relaxation Effect in Rat Aorta." Molecules 25, no. 14: 3105.
Persicaria maculosa (Polygonaceae) (known as lady’s thumb) is an annual morphologically variable weed that is widely distributed in Chile. The purpose of this study was to investigate the antifeedant potential of methanolic (MeOH), ethanolic (EtOH), and dichloromethane (DCM) extracts from the aerial parts of this plant collected in the Valparaíso and Curicó provinces (Chile) and relate this activity to the antioxidant capacity and the presence of phenolic compounds in the extracts. A phenolic profile based on HPLC-ESI-MS/MS allowed the identification of 26 phenolic compounds, most of them glycosyl derivatives of isorhamnetin, quercetin, and kaempferol. In addition, the total phenolic content (TP), total flavonoids (TF), and antioxidant activity measured by 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion scavenging (O2−), ferric-reducing antioxidant power (FRAP), and cupric-reducing antioxidant capacity (CUPRAC) of the extracts are reported. The antifeedant potentials of the plant extracts were tested against Epilachna paenulata, Pseudaletia adultera, Macrosiphum euphorbiae, and Diaphorina citri insects for the first time. The activity against the aphid M. euphorbiae was significant for the DCM extracts of plants from Valparaíso and Curicó (settling % = 23% ± 4% and 23% ± 5%, respectively). The antifeedant activities against the beetle E. paenulata and the lepidoptera P. adultera were significant for Valparaíso extracts, especially when tested against E. Paenulata (IFP = 1.0 ± 0.0). Finally, the MeOH and EtOH extracts from Valparaíso plants reduced the diet consumption of the psilid D. citri (p < 0.05). The results showed that P. maculosa is a good source of flavonoids with some antioxidant capacities and has potential interest as botanical eco-friendly alternative with deterrent activity.
Luisa Quesada-Romero; Carlos Fernández-Galleguillos; Jan Bergmann; María-Eugenia Amorós; Felipe Jiménez-Aspeé; Andrés González; Mario Simirgiotis; Carmen Rossini. Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts. Molecules 2020, 25, 3054 .
AMA StyleLuisa Quesada-Romero, Carlos Fernández-Galleguillos, Jan Bergmann, María-Eugenia Amorós, Felipe Jiménez-Aspeé, Andrés González, Mario Simirgiotis, Carmen Rossini. Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts. Molecules. 2020; 25 (13):3054.
Chicago/Turabian StyleLuisa Quesada-Romero; Carlos Fernández-Galleguillos; Jan Bergmann; María-Eugenia Amorós; Felipe Jiménez-Aspeé; Andrés González; Mario Simirgiotis; Carmen Rossini. 2020. "Phenolic Fingerprinting, Antioxidant, and Deterrent Potentials of Persicaria maculosa Extracts." Molecules 25, no. 13: 3054.
In the present work, the anthelmintic activity (AA) of ethanolic extracts obtained from Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce was evaluated using the third-stage-larval (L3) exsheathment inhibition test (LEIT) and egg hatch test (EHT) on Haemonchus contortus. Extracts were tested at concentrations of 0.3, 0.6, 1.2, 2.5, 5.0, 10, 20, and 40 mg/mL. The larval exsheathment inhibition (LEI) results showed that G. sepium achieved the highest average inhibition of 91.2%, compared with 44.6% for P. dulce and 41.0% for L. leucocephala at a concentration of 40 mg/mL; the corresponding IC50 values were 22.4, 41.7, and 43.3 mg/mL, respectively. The rates of egg hatching inhibition (EHI) at a concentration of 5 mg/mL were 99.5% for G. sepium, 64.2% for P. dulce, and 54% for L. leucocephala; the corresponding IC50 values were 1.9 mg/mL for G. sepium, 3.9 mg/mL for P. dulce, and 4.3 mg/mL for L. leucocephala. The species extracts studied here were also analyzed by ultra-high performance liquid chromatography and Orbitrap high resolution mass spectrometry (UHPLC-Q/Orbitrap/MS/MS), resulting in the compounds’ identification associated with AA. Glycosylated flavonoids and methoxyphenols were observed in all three species: fatty acids in G. sepium and P. dulce; phenylpropanoids, anthraquinone glycosides, amino acids and glycosylated phenolic acids in G. sepium; and flavonoids in L. leucocephala. Comparatively, G. sepium presented a greater diversity of compounds potentially active against the control of gastrointestinal nematodes, which was associated with the results obtained in the applied tests.
Néstor Romero; Carlos Areche; Jaime Cubides-Cárdenas; Natalia Escobar; Olimpo García-Beltrán; Mario J. Simirgiotis; Ángel Céspedes. In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry. Molecules 2020, 25, 3002 .
AMA StyleNéstor Romero, Carlos Areche, Jaime Cubides-Cárdenas, Natalia Escobar, Olimpo García-Beltrán, Mario J. Simirgiotis, Ángel Céspedes. In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry. Molecules. 2020; 25 (13):3002.
Chicago/Turabian StyleNéstor Romero; Carlos Areche; Jaime Cubides-Cárdenas; Natalia Escobar; Olimpo García-Beltrán; Mario J. Simirgiotis; Ángel Céspedes. 2020. "In Vitro Anthelmintic Evaluation of Gliricidia sepium, Leucaena leucocephala, and Pithecellobium dulce: Fingerprint Analysis of Extracts by UHPLC-Orbitrap Mass Spectrometry." Molecules 25, no. 13: 3002.