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Jun Fang
Key Laboratory for Rural Ecosystem Health in Dongting Lake Area of Hunan Province, Changsha 410128, China

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Journal article
Published: 02 May 2021 in Plants
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Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

ACS Style

Shuming Liu; Hongmei Liu; Rui Chen; Yong Ma; Bo Yang; Zhiyong Chen; Yunshan Liang; Jun Fang; Yunhua Xiao. Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.). Plants 2021, 10, 912 .

AMA Style

Shuming Liu, Hongmei Liu, Rui Chen, Yong Ma, Bo Yang, Zhiyong Chen, Yunshan Liang, Jun Fang, Yunhua Xiao. Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.). Plants. 2021; 10 (5):912.

Chicago/Turabian Style

Shuming Liu; Hongmei Liu; Rui Chen; Yong Ma; Bo Yang; Zhiyong Chen; Yunshan Liang; Jun Fang; Yunhua Xiao. 2021. "Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)." Plants 10, no. 5: 912.

Immunology
Published: 01 April 2021 in Frontiers in Immunology
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Epigallocatechin gallate (EGCG) has potent biological activity as well as strong antioxidant and anti-inflammatory effects. This study aims to explore the protective effect of EGCG on LPS-induced acute injury. We randomly divided 18 mice into three groups: CON, LPS, and EGCG-LPS. We gave the EGCG-LPS group gavage treatment with EGCG on day 8–15 and an intraperitoneal injection of LPS on day 16 to induce acute injury. The results showed that, compared with the LPS group, the bodyweight of the mice in the EGCG-LPS group increased significantly and effectively inhibited the morphological damage of the jejunum and liver. We measured liver tissue and found that the EGCG gavage treatment significantly inhibited the pro-inflammatory factors (TNF-α, IL-1β, IL-6, MCP-1, MIP-2, IFN-γ) and oxidation indicators (MPO, NO, ALT, and AST) levels increase. The microbiological results showed that the EGCG gavage treatment reshaped the disturbance done to the intestinal microbial community in the mice by LPS, reversed the changes in the abundance ratio of Firmicutes/Bacteroidetes, and significantly reduced the abundance of Enterobacteriales. Finally, the serum metabolomics results showed that, when compared with the LPS group, the gavage treatment of EGCG significantly increased the concentration of sphingomyelin (d17:1/17:0), sphingomyelin (d16:1/20:0), and significantly reduced the content of trans-Hexadec-2-enoyl carnitine, and so on. Therefore, we believe that EGCG can protect mice from acute stress induced by LPS while stabilizing gut microbes in general, improving the metabolism of sphingolipids, and inhibiting the content of harmful metabolites.

ACS Style

Yong Ma; Gang Liu; Muyang Tang; Jun Fang; Hongmei Jiang. Epigallocatechin Gallate Can Protect Mice From Acute Stress Induced by LPS While Stabilizing Gut Microbes and Serum Metabolites Levels. Frontiers in Immunology 2021, 12, 1 .

AMA Style

Yong Ma, Gang Liu, Muyang Tang, Jun Fang, Hongmei Jiang. Epigallocatechin Gallate Can Protect Mice From Acute Stress Induced by LPS While Stabilizing Gut Microbes and Serum Metabolites Levels. Frontiers in Immunology. 2021; 12 ():1.

Chicago/Turabian Style

Yong Ma; Gang Liu; Muyang Tang; Jun Fang; Hongmei Jiang. 2021. "Epigallocatechin Gallate Can Protect Mice From Acute Stress Induced by LPS While Stabilizing Gut Microbes and Serum Metabolites Levels." Frontiers in Immunology 12, no. : 1.

Review article
Published: 26 December 2020 in Animal Nutrition
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The intestinal immune system is affected by various factors during its development, such as maternal antibodies, host genes, intestinal microbial composition and activity, and various stresses (such as weaning stress). Intestinal microbes may have an important impact on the development of the host immune system. Appropriate interventions such as probiotics may have a positive effect on intestinal immunity by regulating the composition and activity of intestinal microbes. Moreover, probiotics participate in the regulation of host health in many ways; for instance, by improving digestion and the absorption of nutrients, immune response, increasing the content of intestinal-beneficial microorganisms, and inhibiting intestinal-pathogenic bacteria, and they participate in regulating intestinal diseases in various ways. Probiotics are widely used as additives in livestock and the poultry industry and bring health benefits to hosts by improving intestinal microbes and growth performance, which provides more choices for promoting strong and efficient productivity.

ACS Style

Sujuan Ding; Wenxin Yan; Yong Ma; Jun Fang. The impact of probiotics on gut health via alternation of immune status of monogastric animals. Animal Nutrition 2020, 7, 24 -30.

AMA Style

Sujuan Ding, Wenxin Yan, Yong Ma, Jun Fang. The impact of probiotics on gut health via alternation of immune status of monogastric animals. Animal Nutrition. 2020; 7 (1):24-30.

Chicago/Turabian Style

Sujuan Ding; Wenxin Yan; Yong Ma; Jun Fang. 2020. "The impact of probiotics on gut health via alternation of immune status of monogastric animals." Animal Nutrition 7, no. 1: 24-30.

Review article
Published: 09 December 2020 in Oxidative Medicine and Cellular Longevity
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Colorectal cancer (CRC) is the fourth leading cause of cancer-related deaths worldwide and a major global public health problem. With the rapid development of the economy, the incidence of CRC has increased linearly. Accumulating evidence indicates that changes in the gut microenvironment, such as undesirable changes in the microbiota composition, provide favorable conditions for intestinal inflammation and shaping the tumor growth environment, whereas administration of certain probiotics can reverse this situation to a certain extent. This review summarizes the roles of probiotics in the regulation of CRC, such as enhancing the immune barrier, regulating the intestinal immune state, inhibiting pathogenic enzyme activity, regulating CRC cell proliferation and apoptosis, regulating redox homeostasis, and reprograming intestinal microbial composition. Abundant studies have provided a theoretical foundation for the roles of probiotics in CRC prevention and treatment, but their mechanisms of action remain to be investigated, and further clinical trials are warranted for the application of probiotics in the target population.

ACS Style

Sujuan Ding; Chao Hu; Jun Fang; Gang Liu. The Protective Role of Probiotics against Colorectal Cancer. Oxidative Medicine and Cellular Longevity 2020, 2020, 1 -10.

AMA Style

Sujuan Ding, Chao Hu, Jun Fang, Gang Liu. The Protective Role of Probiotics against Colorectal Cancer. Oxidative Medicine and Cellular Longevity. 2020; 2020 ():1-10.

Chicago/Turabian Style

Sujuan Ding; Chao Hu; Jun Fang; Gang Liu. 2020. "The Protective Role of Probiotics against Colorectal Cancer." Oxidative Medicine and Cellular Longevity 2020, no. : 1-10.

Research article
Published: 23 November 2020 in Environmental Science and Pollution Research
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The total contents and chemical speciation analysis of Zn, Cu, Pb, Cr, Mn, Ni, Cd, and As in pig manure (PM), liquefaction residues (LRs), and bio-oils (BOs) derived from PM by liquefaction with ethanol as a solvent at 180–300 °C were thoroughly investigated in this study. The environment risk assessment, leachability, and bioavailability of heavy metals in PM and LRs were studied. The results showed that more than 75% of heavy metals remained in LRs. The total contents of heavy metals in LRs were markedly elevated, but those in BOs gradually decreased with the increase in liquefaction temperature. Moreover, the acid soluble/exchangeable fraction and reducible fraction (F1 + F2) of heavy metals in LRs and BOs was significantly reduced, while oxidizable fraction and stable fraction (F3 + F4) desirably increased after liquefaction. Furthermore, the potential risk of heavy metals in LRs was decreased in comparison to that in PM, but the risk of Pb, Mn, and As had not been obviously reduced; therefore, the LRs from the liquefaction of PM should be pretreated before recycling. Temperatures from 220 to 260 °C were the optimum conditions for disposing of PM by liquefaction with ethanol.

ACS Style

Hui Luan; Fen Liu; Shundong Long; Zhuangzhuang Liu; Yanting Qi; Zhihua Xiao; Jun Fang. The migration, transformation, and risk assessment of heavy metals in residue and bio-oil obtained by the liquefaction of pig manure. Environmental Science and Pollution Research 2020, 28, 15055 -15069.

AMA Style

Hui Luan, Fen Liu, Shundong Long, Zhuangzhuang Liu, Yanting Qi, Zhihua Xiao, Jun Fang. The migration, transformation, and risk assessment of heavy metals in residue and bio-oil obtained by the liquefaction of pig manure. Environmental Science and Pollution Research. 2020; 28 (12):15055-15069.

Chicago/Turabian Style

Hui Luan; Fen Liu; Shundong Long; Zhuangzhuang Liu; Yanting Qi; Zhihua Xiao; Jun Fang. 2020. "The migration, transformation, and risk assessment of heavy metals in residue and bio-oil obtained by the liquefaction of pig manure." Environmental Science and Pollution Research 28, no. 12: 15055-15069.

Journal article
Published: 08 August 2020 in Nanomaterials
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Nitrogen-doped carbon quantum dots (N-CQDs) with strong fluorescence were prepared by a one-step hydrothermal method using natural biomass waste. Two efficient fluorescent probes were constructed for selective and sensitive detection of oxytetracycline (OTC). The synthesized N-CQDs were characterized by UV-visible absorption spectra, fluorescence spectra, Fourier transform infrared spectroscopy (FT-IR), X-ray photon spectroscopy (XPS), atomic force microscopy (AFM), and high-resolution transmission electron microscopy (HRTEM), which proved that the synthesized N-CQDs surface were functionalized and had stable fluorescence performance. The basis of N-CQDs detection of OTC was discussed, and various reaction conditions were studied. Under optimized conditions, orange peel carbon quantum dots (ON-CQDs) and watermelon peel carbon quantum dots (WN-CQDs) have a good linear relationship with OTC concentrations in the range of 2–100 µmol L−1 and 0.25–100 µmol L−1, respectively. ON-CQDs and WN-CQDs were both successfully applied in detecting the OTC in pretreated tap water, lake water, and soil, with the recovery rate at 91.724–103.206%, and the relative standard deviation was less than 5.35%. The results showed that the proposed N-CQDs proved to be green and simple, greatly reducing the detection time for OTC in the determination environment.

ACS Style

Rong Gao; Zhibin Wu; Li Wang; Jiao Liu; Yijun Deng; Zhihua Xiao; Jun Fang; Yunshan Liang. Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples. Nanomaterials 2020, 10, 1561 .

AMA Style

Rong Gao, Zhibin Wu, Li Wang, Jiao Liu, Yijun Deng, Zhihua Xiao, Jun Fang, Yunshan Liang. Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples. Nanomaterials. 2020; 10 (8):1561.

Chicago/Turabian Style

Rong Gao; Zhibin Wu; Li Wang; Jiao Liu; Yijun Deng; Zhihua Xiao; Jun Fang; Yunshan Liang. 2020. "Green Preparation of Fluorescent Nitrogen-Doped Carbon Quantum Dots for Sensitive Detection of Oxytetracycline in Environmental Samples." Nanomaterials 10, no. 8: 1561.

Review article
Published: 10 July 2020 in Frontiers in Immunology
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Colorectal cancer (CRC) is one of the most prevalent cancers that threaten people in many countries. It is a multi-factorial chronic disease caused by a combination of genetic and environmental factors, but it is mainly related to lifestyle factors, including diet. Plentiful plant foods and beverages are abundant in polyphenols with antioxidant, anti-atherosclerotic, anti-inflammatory, and anticancer properties. These compounds participate in host nutrition and disease pathology regulation in different ways. Polyphenolic compounds have been used to prevent and inhibit the development and prognosis of cancer, and examples include green tea polyphenol (–)epigallocatechin-3-O-gallate (EGCG), curcumin, and resveratrol. Of course, there are more known and unknown polyphenol compounds that need to be further explored for their anticancer properties. This article focuses on the fact that polyphenols affect the progression of CRC by controlling intestinal inflammation, epigenetics, and the intestinal microbe in the aspects of prevention, treatment, and prognosis.

ACS Style

Sujuan Ding; Sheng Xu; Jun Fang; Hongmei Jiang. The Protective Effect of Polyphenols for Colorectal Cancer. Frontiers in Immunology 2020, 11, 1407 .

AMA Style

Sujuan Ding, Sheng Xu, Jun Fang, Hongmei Jiang. The Protective Effect of Polyphenols for Colorectal Cancer. Frontiers in Immunology. 2020; 11 ():1407.

Chicago/Turabian Style

Sujuan Ding; Sheng Xu; Jun Fang; Hongmei Jiang. 2020. "The Protective Effect of Polyphenols for Colorectal Cancer." Frontiers in Immunology 11, no. : 1407.

Review article
Published: 15 April 2020 in BioMed Research International
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Pyrimidine nucleoside uridine plays a critical role in maintaining cellular function and energy metabolism. In addition to its role in nucleoside synthesis, uridine and its derivatives contribute to reduction of cytotoxicity and suppression of drug-induced hepatic steatosis. Uridine is mostly present in blood and cerebrospinal fluid, where it contributes to the maintenance of basic cellular functions affected by UPase enzyme activity, feeding habits, and ATP depletion. Uridine metabolism depends on three stages: de novo synthesis, salvage synthesis pathway and catabolism, and homeostasis, which is tightly relating to glucose homeostasis and lipid and amino acid metabolism. This review is devoted to uridine metabolism and its role in glucose, lipid, and amino acid homeostasis.

ACS Style

Yumei Zhang; Songge Guo; Chunyan Xie; Jun Fang. Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis. BioMed Research International 2020, 2020, 1 -7.

AMA Style

Yumei Zhang, Songge Guo, Chunyan Xie, Jun Fang. Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis. BioMed Research International. 2020; 2020 ():1-7.

Chicago/Turabian Style

Yumei Zhang; Songge Guo; Chunyan Xie; Jun Fang. 2020. "Uridine Metabolism and Its Role in Glucose, Lipid, and Amino Acid Homeostasis." BioMed Research International 2020, no. : 1-7.

Erratum
Published: 20 March 2020 in Fuel
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ACS Style

Qingdan Wu; Hui Luan; Hua Wang; Xiaochen Zheng; Fen Liu; Andong Wang; Dongsheng Zou; Jinrui Yuan; Zhihua Xiao; Jun Fang. Corrigendum to “Thermochemical liquefaction of pig manure: Factors influencing on oil” [Fuel 264 (2020) 116884]. Fuel 2020, 271, 117627 .

AMA Style

Qingdan Wu, Hui Luan, Hua Wang, Xiaochen Zheng, Fen Liu, Andong Wang, Dongsheng Zou, Jinrui Yuan, Zhihua Xiao, Jun Fang. Corrigendum to “Thermochemical liquefaction of pig manure: Factors influencing on oil” [Fuel 264 (2020) 116884]. Fuel. 2020; 271 ():117627.

Chicago/Turabian Style

Qingdan Wu; Hui Luan; Hua Wang; Xiaochen Zheng; Fen Liu; Andong Wang; Dongsheng Zou; Jinrui Yuan; Zhihua Xiao; Jun Fang. 2020. "Corrigendum to “Thermochemical liquefaction of pig manure: Factors influencing on oil” [Fuel 264 (2020) 116884]." Fuel 271, no. : 117627.

Review article
Published: 16 March 2020 in Environmental Science and Pollution Research
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Accumulation of heavy metals in agricultural soils due to human production activities-mining, fossil fuel combustion, and application of chemical fertilizers/pesticides-results in severe environmental pollution. As the transmission of heavy metals through the food chain and their accumulation pose a serious risk to human health and safety, there has been increasing attention in the investigation of heavy metal pollution and search for effective soil remediation technologies. Here, we summarized and discussed the basic principles, strengths and weaknesses, and limitations of common standalone approaches such as those based on physics, chemistry, and biology, emphasizing their incompatibility with large-scale applications. Moreover, we explained the effects, advantages, and disadvantages of the combinations of common single repair approaches. We highlighted the latest research advances and prospects in phytoremediation-chemical, phytoremediation-microbe, and phytoremediation-genetic engineering combined with remediation approaches by changing metal availability, improving plant tolerance, promoting plant growth, improving phytoextraction and phytostabilization, etc. We then explained the improved safety and applicability of phytoremediation combined with other repair approaches compared to common standalone approaches. Finally, we established a prospective research direction of phytoremediation combined with multi-technology repair strategy.

ACS Style

Shuming Liu; Bo Yang; Yunshan Liang; Yunhua Xiao; Jun Fang. Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils. Environmental Science and Pollution Research 2020, 27, 16069 -16085.

AMA Style

Shuming Liu, Bo Yang, Yunshan Liang, Yunhua Xiao, Jun Fang. Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils. Environmental Science and Pollution Research. 2020; 27 (14):16069-16085.

Chicago/Turabian Style

Shuming Liu; Bo Yang; Yunshan Liang; Yunhua Xiao; Jun Fang. 2020. "Prospect of phytoremediation combined with other approaches for remediation of heavy metal-polluted soils." Environmental Science and Pollution Research 27, no. 14: 16069-16085.

Review
Published: 09 December 2019 in Biomolecules
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The inflammasome is a multiprotein complex that acts to enhance inflammatory responses by promoting the production and secretion of key cytokines. The best-known inflammasome is the NLRP3 (nucleotide-binding oligomerization domain-like receptor [NLR] family pyrin domain-containing 3) inflammasome. The evidence has shown that the NLRP3 inflammasome, IL-1β, thioredoxin-interacting protein (TXNIP), and pyroptosis play vital roles in the development of diabetes. This review summarizes the regulation of type 1 diabetes mellitus (T1DM) and type 2 diabetes mellitus (T2DM) by NLRP3 via modulation of glucose tolerance, insulin resistance, inflammation, and apoptosis mediated by endoplasmic reticulum stress in adipose tissue. Moreover, NLRP3 participates in intestinal homeostasis and inflammatory conditions, and NLRP3-deficient mice experience intestinal lesions. The diversity of an individual’s gut microbiome and the resultant microbial metabolites determines the extent of their involvement in the physiological and pathological mechanisms within the gut. As such, further study of the interaction between the NLRP3 inflammasome and the complex intestinal environment in disease development is warranted to discover novel therapies for the treatment of diabetes.

ACS Style

Sujuan Ding; Sheng Xu; Yong Ma; Gang Liu; Hongmei Jang; Jun Fang. Modulatory Mechanisms of the NLRP3 Inflammasomes in Diabetes. Biomolecules 2019, 9, 850 .

AMA Style

Sujuan Ding, Sheng Xu, Yong Ma, Gang Liu, Hongmei Jang, Jun Fang. Modulatory Mechanisms of the NLRP3 Inflammasomes in Diabetes. Biomolecules. 2019; 9 (12):850.

Chicago/Turabian Style

Sujuan Ding; Sheng Xu; Yong Ma; Gang Liu; Hongmei Jang; Jun Fang. 2019. "Modulatory Mechanisms of the NLRP3 Inflammasomes in Diabetes." Biomolecules 9, no. 12: 850.

Research article
Published: 24 October 2019 in BioMed Research International
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Background and Objective. Bioactive peptides exert great influence in animals and human health by targeting gastrointestinal tracts. The colitis model of mice was induced by dextran sulfate sodium (DSS). Thirty-two 8-week-old mice weighing 23 g on average were randomly assigned to four groups of 8 each: mice fed basal diet (CON), mice fed basal diet with 5% DSS (DSS), mice fed 0.03% IRW with 5% DSS (IRW-DSS), and mice fed 0.03% IRW with 5% DSS (IQW-DSS). After an adaptation period of 3 days, on day 8, all mice were slaughtered. Serum samples were collected to determine the level of amino acids; colonic tissue was quick-frozen for the determination of gene expression. Methods. The aim of this study was to assess the ability of two kinds of peptides (IRW and IQW) to repair intestinal inflammatory in the DSS-induced model in accordance with serum amino acids and intestinal inflammatory factors. Results. The results demonstrated that the addition of IRW and IQW had a mitigating effect on DSS-induced intestinal inflammation. The level of Asp decreased in the serum of mice supplemented with IRW-DSS (P<0.05), and IQW enhanced the level of Leu, but lowered the level of Ser (P<0.05). IQW and IRW addition reduced the level of TNF-α and IL-17 (P<0.05). No other significant effects were observed. Conclusions. The present study demonstrated that intracolic administration of IRW and IQW might be a novel option for preventing inflammatory bowel disease via regulating the level of serum amino acid and enhancing the intestinal immune defense.

ACS Style

Yong Ma; Hongmei Jiang; Jun Fang; Gang Liu. IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation. BioMed Research International 2019, 2019, 1 -9.

AMA Style

Yong Ma, Hongmei Jiang, Jun Fang, Gang Liu. IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation. BioMed Research International. 2019; 2019 ():1-9.

Chicago/Turabian Style

Yong Ma; Hongmei Jiang; Jun Fang; Gang Liu. 2019. "IRW and IQW Reduce Colitis-Associated Cancer Risk by Alleviating DSS-Induced Colonic Inflammation." BioMed Research International 2019, no. : 1-9.

Original research article
Published: 18 September 2019 in Frontiers in Physiology
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The aim of this study was to examine the effects of Lactobacillus brevis on the microbial community and serum metabolome in colitis induced by dextran sulfate sodium (DSS). ICR mice were randomly distributed into three treatment groups: (i) L. brevis treatment alone (control), (ii) DSS administration alone, and (iii) treatment with L. brevis and DSS. Our results demonstrate that L. brevis treatment significantly alleviated DSS-induced body weight loss and colon inflammation. In addition, LC-MS analysis of serum metabolites revealed that L. brevis treatment increased the serum level of metabolites against inflammatory responses or oxidative stressors caused by DSS in the murine model. By detecting colonic microbiota, L. brevis increased colonic microbial diversity after challenging with DSS, and increased the relative abundance of Alloprevotella at genus, but Bacteroidales was reduced (P < 0.05). These result indicated that L. brevis could lower the severity of colitis induced by DSS via improving reprogramming the serum metabolome and intestinal microbiota. These findings suggest that the probiotic L. brevis may prevent tissue damage from colitis.

ACS Style

Sujuan Ding; Yong Ma; Gang Liu; Wenxin Yan; Hongmei Jiang; Jun Fang. Lactobacillus brevis Alleviates DSS-Induced Colitis by Reprograming Intestinal Microbiota and Influencing Serum Metabolome in Murine Model. Frontiers in Physiology 2019, 10, 1152 .

AMA Style

Sujuan Ding, Yong Ma, Gang Liu, Wenxin Yan, Hongmei Jiang, Jun Fang. Lactobacillus brevis Alleviates DSS-Induced Colitis by Reprograming Intestinal Microbiota and Influencing Serum Metabolome in Murine Model. Frontiers in Physiology. 2019; 10 ():1152.

Chicago/Turabian Style

Sujuan Ding; Yong Ma; Gang Liu; Wenxin Yan; Hongmei Jiang; Jun Fang. 2019. "Lactobacillus brevis Alleviates DSS-Induced Colitis by Reprograming Intestinal Microbiota and Influencing Serum Metabolome in Murine Model." Frontiers in Physiology 10, no. : 1152.

Research article
Published: 13 June 2019 in PLOS ONE
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One-day-old broilers were randomly allocated to five treatment groups: basal diet and orally administered sterile saline (negative control, n-control); basal diet challenged with E. coli O78 (positive control, p-control); basal diet supplemented with 1×108 CFU/kg L. plantarum 15–1 and challenged with E. coli O78 (LP); basal diet supplemented with 5 g/kg fructooligosaccharides (FOS) and challenged with E. coli O78 (FOS); and basal diet supplemented with both L. plantarum 15–1 and FOS and challenged with E. coli O78 (LP+FOS). The broilers in the LP, FOS, and LP+FOS groups displayed a decrease of crypt depth at day 14 compared with the control groups. Furthermore, at days 14 and 21, the broilers in the LP group exhibited reduced serum levels of diamine oxidase (DAO) compared with the p-control group (p<0.05), and the broilers in the LP+FOS group showed increased serum concentrations of IgA and IgG relative to both control groups and decreased DAO levels compared with the p-control group (p<0.05). Moreover, the LP group displayed higher levels of acetic acid and total short-chain fatty acids (SCFAs) compared with the p-control group at day 14 (p<0.05), and the FOS group showed higher levels of valeric acid and total SCFAs at day 21 (p<0.05). The LP+FOS group also displayed a higher level of butyric acid at day 14 (p<0.05). In conclusion, dietary supplementation with FOS improved the growth performance, while supplementation with L. plantarum 15–1 and FOS improved intestinal health by increasing the levels of SCFAs and mitigating the damage caused by E. coli O78, thus preventing intestinal damage and enhancing the immune response.

ACS Style

Sujuan Ding; Yongwei Wang; Wenxin Yan; Aike Li; Hongmei Jiang; Jun Fang. Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge. PLOS ONE 2019, 14, e0212079 .

AMA Style

Sujuan Ding, Yongwei Wang, Wenxin Yan, Aike Li, Hongmei Jiang, Jun Fang. Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge. PLOS ONE. 2019; 14 (6):e0212079.

Chicago/Turabian Style

Sujuan Ding; Yongwei Wang; Wenxin Yan; Aike Li; Hongmei Jiang; Jun Fang. 2019. "Effects of Lactobacillus plantarum 15-1 and fructooligosaccharides on the response of broilers to pathogenic Escherichia coli O78 challenge." PLOS ONE 14, no. 6: e0212079.

Journal article
Published: 13 June 2019 in Food Control
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The main foodborne pathogens are Norovirus, Enteric parasites, bacteria and fungi. Foodborne diseases can lead to high morbidity and mortality and result in huge economic losses. Some foodborne diseases are associated with antibiotic resistance, which is a significant problem throughout the world. To reduce the harm caused by antibiotics, it is important to find alternatives so that food is not infected with foodborne pathogens. Anthocyanins and catechins, two species of plant polyphenols with strong antioxidant properties, offer a promising new approach to preventing foodborne disease. Recent studies have shown that these compounds are good growth inhibitors. In this review, we focus on the antibacterial properties of anthocyanins and catechins as antibiotic substitutes. The minimum inhibitory concentration (MIC) of anthocyanins against E. coli and Salmonella is 10 - 400 mg/ml. The MIC of catechins against E. coli and Salmonella is 6 - 50 mg/ml. Anthocyanins and catechins can also significantly reduce the abundance of pathogenic bacteria that produce toxins in the host including Desulfovibrio sp. and Enterococcus. At the same time, we summarize the antibacterial mechanism of Anthocyanins and catechins on Salmonella and Escherichia coli. Anthocyanins and catechins can increase the abundance of probiotics such as Akkermansia, Bifidobacteria, and increase the ratio of thick-walled bacteria to Bacteroides. Finally, it seems that Anthocyanins and catechins can regulate the composition of intestinal microbes to improve intestinal immunity and promote intestinal health, thereby controlling foodborne

ACS Style

Yong Ma; Sujuan Ding; Yanquan Fei; Gang Liu; Hongmei Jang; Jun Fang. Antimicrobial activity of anthocyanins and catechins against foodborne pathogens Escherichia coli and Salmonella. Food Control 2019, 106, 106712 .

AMA Style

Yong Ma, Sujuan Ding, Yanquan Fei, Gang Liu, Hongmei Jang, Jun Fang. Antimicrobial activity of anthocyanins and catechins against foodborne pathogens Escherichia coli and Salmonella. Food Control. 2019; 106 ():106712.

Chicago/Turabian Style

Yong Ma; Sujuan Ding; Yanquan Fei; Gang Liu; Hongmei Jang; Jun Fang. 2019. "Antimicrobial activity of anthocyanins and catechins against foodborne pathogens Escherichia coli and Salmonella." Food Control 106, no. : 106712.

Original research article
Published: 03 April 2019 in Frontiers in Microbiology
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Bioactive peptides that target the gastrointestinal tract can strongly affect the health of animals and humans. This study aimed to evaluate the abilities of two peptides derived from egg albumin transferrin, IRW and IQW, to treat enteritis in a mouse model of Citrobacter rodentium-induced colitis by evaluating serum metabolomics and gut microbes. Forty-eight mice were randomly assigned to six groups: basal diet (CTRL), intragastric administration Citrobacter rodentium (CR), basal diet with 0.03%IRW (IRW), CR with 0.03% IRW (IRW+CR), basal diet with 0.03%IQW (IQW) and CR with 0.03% IQW (IQW+CR). CR administration began on day 10 and continued for 7 days. After 14 days of IRW and IQW treatment, serum was collected and subjected to a metabolomics analysis. The length and weight of each colon were measured, and the colon contents were collected for 16srRNA sequencing. The colons were significantly longer in the CR group, compared to the CTRL group. A serum metabolomics analysis revealed no significant difference in microbial diversity between the six groups. Compared with the CTRL group, the proportions of Firmicutes and Actinobacteria species decreased significantly and the proportions of Bacteroidetes and Proteobacteria species increased in the CR group. There were no significant differences between the CTRL and other groups. The serum metabolomics analysis revealed that Infected by CR increased the levels of oxalic acid, homogentisic acid and prostaglandin but decreased the levels of L-glutamine, L-acetyl carnitine, 1-methylhistidine and gentisic acid. Therefore, treatment with IRW and IQW was shown to regulate the intestinal microorganisms associated with colonic inflammation and serum metabolite levels, thus improving intestinal health.

ACS Style

Yong Ma; Sujuan Ding; Gang Liu; Jun Fang; Wenxin Yan; Veeramuthu Duraipandiyan; Naif Abdullah Al-Dhabi; Galal Ali Esmail; Hongmei Jiang. Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related Microbial and Metabolomic Profiles. Frontiers in Microbiology 2019, 10, 643 .

AMA Style

Yong Ma, Sujuan Ding, Gang Liu, Jun Fang, Wenxin Yan, Veeramuthu Duraipandiyan, Naif Abdullah Al-Dhabi, Galal Ali Esmail, Hongmei Jiang. Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related Microbial and Metabolomic Profiles. Frontiers in Microbiology. 2019; 10 ():643.

Chicago/Turabian Style

Yong Ma; Sujuan Ding; Gang Liu; Jun Fang; Wenxin Yan; Veeramuthu Duraipandiyan; Naif Abdullah Al-Dhabi; Galal Ali Esmail; Hongmei Jiang. 2019. "Egg Protein Transferrin-Derived Peptides IRW and IQW Regulate Citrobacter rodentium-Induced, Inflammation-Related Microbial and Metabolomic Profiles." Frontiers in Microbiology 10, no. : 643.

Preprint
Published: 29 January 2019
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One-day-old broilers were randomly allocated to 5 treatments: basal diet challenged by saline (negative control, n-control); basal diet and challenged by E.coil O78 (positive control, p-control); supplementation with L. plantarum 15-1 at 1×108 CFU/kg challenged with E.coil O78 (LP); supplementation with FOS at 5 g/kg challenged with E.coil O78 (FOS); supplementation with L. plantarum 15-1 and FOS challenged with E.coil O78 (LP+FOS). L. plantarum 15-1 or FOS had a lowered effect (PL. plantarum 15-1, FOS and L. plantarum 15-1+FOS also reduced relative to p-control on d 21. L. plantarum 15-1 reduced the level of diamine oxidase (DAO) at d 14 and 21 compared with p-control (PL. plantarum 15-1 and FOS increased the concentration of IgA and IgG relative to two control, and decreased diamine oxidase (DAO) compared with p-control (P 0.05). L. plantarum 15-1 increased the concentration of acetic acid and total short-chain fatty acid (SCFA) in comparison with p-control at d 14 (PPL. plantarum 15-1 and FOS were increased the level of butyric acid at d 14 (PPL. plantarum 15-1 and FOS did no effect on the growth performance. In conclusion, FOS can promote average daily gain, L. plantarum 15-1 and FOS can improve intestinal morphology, and increase the concentration of SCFA in cecal contents in broilers challenged with E.coil O78. These results suggest that L. plantarum 15-1 and FOS have effective mitigation to E. coil O78 via lowing reducing the intestinal injury and enhancing the immune responses.

ACS Style

Sujuan Ding; Yongwei Wang; Wenxin Yan; Aike Li; Hongmei Jiang; Jun Fang. Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O78 challenge. 2019, 533935 .

AMA Style

Sujuan Ding, Yongwei Wang, Wenxin Yan, Aike Li, Hongmei Jiang, Jun Fang. Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O78 challenge. . 2019; ():533935.

Chicago/Turabian Style

Sujuan Ding; Yongwei Wang; Wenxin Yan; Aike Li; Hongmei Jiang; Jun Fang. 2019. "Effects of Lactobacillus plantarum 15-1 and fructooligosaccharide on the response of broilers to pathogenic Escherichia coli O78 challenge." , no. : 533935.

Corrigendum
Published: 13 December 2018 in Oxidative Medicine and Cellular Longevity
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[This corrects the article DOI: 10.1155/2017/6504518.].

ACS Style

Shuang Chen; Hongmei Jiang; Hanhui Peng; Xiaosong Wu; Jun Fang. Corrigendum to “The Utility of Ovotransferrin and Ovotransferrin-Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases”. Oxidative Medicine and Cellular Longevity 2018, 2018, 1 -2.

AMA Style

Shuang Chen, Hongmei Jiang, Hanhui Peng, Xiaosong Wu, Jun Fang. Corrigendum to “The Utility of Ovotransferrin and Ovotransferrin-Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases”. Oxidative Medicine and Cellular Longevity. 2018; 2018 ():1-2.

Chicago/Turabian Style

Shuang Chen; Hongmei Jiang; Hanhui Peng; Xiaosong Wu; Jun Fang. 2018. "Corrigendum to “The Utility of Ovotransferrin and Ovotransferrin-Derived Peptides as Possible Candidates in the Clinical Treatment of Cardiovascular Diseases”." Oxidative Medicine and Cellular Longevity 2018, no. : 1-2.

Journal article
Published: 19 November 2018 in Cellular Physiology and Biochemistry
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Background/Aims: There are known links between inflammatory bowel disease (IBD) and changes in the microbiota of the gut and inflammation and oxidative stress. In this study, a colitis model induced by dextran sodium sulfate (DSS) in mice is used to evaluate whether the presence of bioactive peptides IRW (Ile-Arg-Trp) and IQW (Ile-Gln-Trp) peptides is advantageous. Methods: The mice were arbitrarily assigned to the following four groups: (i) control (untreated), (ii) dextran sodium sulfate (DSS) treated, (iii) IRW-DSS treated, and (iv) IQW-DSS treated. For 7 days, the control group subjects had unrestricted access to untreated drinking water, whereas the drinking water supplied to the subjects in the DSS, IRW-DSS, and IQW-DSS groups during this period consisted of 5% DSS solution. The colonic lesions were scored after hematoxylin and eosin staining. Serum antioxidant capacity was analyzed by 2,2’-azino-bis(3-ethylbenz-thiazoline-6-sulfonic acid) (ABTS) radical cation decolorization test and the microbiota in the colonic contents were sequenced by HiSeq2500 PE250. Results: The presence of DSS reduced daily weight gain, enhanced histopathology scores, and inhibited antioxidant enzyme expression. Superoxide dismutase, catalase, and glutathione peroxidase activities in the DSS-induced colitis model were significantly enhanced (P < 0.05) in the presence of dietary IRW and IQW. Furthermore, the Simpson index was significantly increased (P < 0.05) in the presence of dietary IRW and IQW compared to the control group. IRW and IQW increased the abundance of Coprococcus_1, Ruminococcaceae_UCG-014, and Desulfovibrio compared to the control group and DSS group. Furthermore, IQW decreased the abundance of Bacteroides in relation to the control group, but increased Parabacteroides. In addition, IRW increased the level of Anaerotruncus, Oscillibacter, and Ruminiclostridium_9 compared to the control group. Conclusion: This study concludes that the presence of IRW or IQW can mitigate DSS-induced oxidative stress by improving the activities of antioxidant enzymes, increasing intestinal microbial diversity and enhancing the abundance of gut microbiota, which may help maintain the homeostasis of host health and microenvironment in a DSS-induced mouse model, thus providing a potential further treatment for IBD patients.

ACS Style

Gang Liu; Wenxin Yan; Sujuan Ding; Hongmei Jiang; Yong Ma; Hua Wang; Jun Fang. Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis. Cellular Physiology and Biochemistry 2018, 51, 441 -451.

AMA Style

Gang Liu, Wenxin Yan, Sujuan Ding, Hongmei Jiang, Yong Ma, Hua Wang, Jun Fang. Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis. Cellular Physiology and Biochemistry. 2018; 51 (1):441-451.

Chicago/Turabian Style

Gang Liu; Wenxin Yan; Sujuan Ding; Hongmei Jiang; Yong Ma; Hua Wang; Jun Fang. 2018. "Effects of IRW and IQW on Oxidative Stress and Gut Microbiota in Dextran Sodium Sulfate-Induced Colitis." Cellular Physiology and Biochemistry 51, no. 1: 441-451.

Research article
Published: 04 August 2018 in Environmental Science and Pollution Research
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The aim of this study is to explore the impact of cadmium (Cd) and arsenic (As) stress on physiological indexes and Cd/As uptake ability of Miscanthus, including Miscanthus sacchariflorus A0104, Miscanthus sinensis C0424 and C0640. Cd and As concentration showed significant hormesis effects on some physiological indexes, such as chlorophyll content, net photosynthetic rate, and MDA content. Compared with control group, Cd uptake can be most greatly enhanced by above fourfold with 150 mg/kg Cd treatment. As uptake of A0104 was increased by 3 ~ 33 folds with 200 mg/kg As treatment, C0424 was increased by 7–12 folds with 100 mg/kg As treatment, while C0640 was increased 1 ~ 6 folds with 250 mg/kg As treatment. The results also showed that C0640 was relatively better for the Cd remediation in the high Cd concentration (150 mg/kg) contaminated soil, while A0104 and C0424 were relatively better for the As remediation in the high As concentration (100 ~ 200 mg/kg) contaminated soil. Additionally, significantly (p < 0.05) close correlations were found among physiological indexes (except MDA content), while physiological indexes showed no significant relationship with the heavy metal contents in root, stem, and leaf.

ACS Style

Hongmei Jiang; Xiaohui Zhao; Jun Fang; Yunhua Xiao. Physiological responses and metal uptake of Miscanthus under cadmium/arsenic stress. Environmental Science and Pollution Research 2018, 25, 28275 -28284.

AMA Style

Hongmei Jiang, Xiaohui Zhao, Jun Fang, Yunhua Xiao. Physiological responses and metal uptake of Miscanthus under cadmium/arsenic stress. Environmental Science and Pollution Research. 2018; 25 (28):28275-28284.

Chicago/Turabian Style

Hongmei Jiang; Xiaohui Zhao; Jun Fang; Yunhua Xiao. 2018. "Physiological responses and metal uptake of Miscanthus under cadmium/arsenic stress." Environmental Science and Pollution Research 25, no. 28: 28275-28284.