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Squash mosaic virus (SqMV) is a phytovirus that infects great diversity of plants worldwide. In Brazil, the SqMV has been identified in the states of Ceará, Maranhão, Piauí, Rio Grande do Norte, and Tocantins. The presence of non-pathogenic viruses in animals, such as phytoviruses, may not be completely risk-free. Similarities in gene repertories between these viruses and viruses that affect animal species have been reported. The present study describes the fully sequenced genomes of SqMV found in human feces, collected in Tocantins, and analyzes the viral profile by metagenomics in the context of diarrhea symptomatology. The complete SqMV genome was obtained in 39 of 253 analyzed samples (15.5%); 97.4% of them belonged to children under 5 years old. There was no evidence that the observed symptoms were related to the presence of SqMV. Of the different virus species detected in these fecal samples, at least 4 (rotavirus, sapovirus, norovirus, parechovirus) are widely known to cause gastrointestinal symptoms. The presence of SqMV nucleic acid in fecal samples is likely due to recent dietary consumption and it is not evidence of viral replication in the human intestinal cells. Identifying the presence of SqMV in human feces and characterization of its genome is a relevant precursor to determining whether and how plant viruses interact with host cells or microorganisms in the human gastrointestinal tract.
Fabiola Villanova; Roberta Marcatti; Mayara Bertanhe; Vanessa Morais; Flavio Milagres; Rafael Brustulin; Emerson Araújo; Roozbeh Tahmasebi; Steven Witkin; Xutao Deng; Eric Delwart; Ester Sabino; Cassio Abreu-Junior; Élcio Leal; Antonio Costa. New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples. Microorganisms 2021, 9, 1349 .
AMA StyleFabiola Villanova, Roberta Marcatti, Mayara Bertanhe, Vanessa Morais, Flavio Milagres, Rafael Brustulin, Emerson Araújo, Roozbeh Tahmasebi, Steven Witkin, Xutao Deng, Eric Delwart, Ester Sabino, Cassio Abreu-Junior, Élcio Leal, Antonio Costa. New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples. Microorganisms. 2021; 9 (7):1349.
Chicago/Turabian StyleFabiola Villanova; Roberta Marcatti; Mayara Bertanhe; Vanessa Morais; Flavio Milagres; Rafael Brustulin; Emerson Araújo; Roozbeh Tahmasebi; Steven Witkin; Xutao Deng; Eric Delwart; Ester Sabino; Cassio Abreu-Junior; Élcio Leal; Antonio Costa. 2021. "New Variants of Squash Mosaic Viruses Detected in Human Fecal Samples." Microorganisms 9, no. 7: 1349.
Dengue virus (DENV) is a mosquito-borne viral pathogen that plagues many tropical-climate nations around the world, including Brazil. Molecular epidemiology is a growing and increasingly invaluable tool for understanding the dispersal, persistence, and diversity of this impactful virus. In this study, plasma samples (n = 824) from individuals with symptoms consistent with an arboviral febrile illness were analyzed to identity the molecular epidemiological dynamics of DENV circulating in the Brazilian state of Amapá. Twelve DENV type 1 (DENV-1) genomes were identified, which were phylogenetically related to the BR4 lineage of genotype V. Phylodynamics analysis suggested that DENV-1 BR-4 was introduced into Amapá around early 2010, possibly from other states in northern Brazil. We also found unique amino acids substitutions in the DENV-1 envelope and NS5 protein sequences in the Amapá isolates. Characterization of the DENV-1 BR-4 sequences highlights the potential of this new lineage to drive outbreaks of dengue in the Amazon region.
Geovani Ribeiro; Danielle Gill; Edcelha Ribeiro; Fred Monteiro; Vanessa Morais; Roberta Marcatti; Marlisson Rego; Emerson Araújo; Steven Witkin; Fabiola Villanova; Xutao Deng; Ester Sabino; Eric Delwart; Élcio Leal; Antonio da Costa. Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon. Viruses 2021, 13, 689 .
AMA StyleGeovani Ribeiro, Danielle Gill, Edcelha Ribeiro, Fred Monteiro, Vanessa Morais, Roberta Marcatti, Marlisson Rego, Emerson Araújo, Steven Witkin, Fabiola Villanova, Xutao Deng, Ester Sabino, Eric Delwart, Élcio Leal, Antonio da Costa. Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon. Viruses. 2021; 13 (4):689.
Chicago/Turabian StyleGeovani Ribeiro; Danielle Gill; Edcelha Ribeiro; Fred Monteiro; Vanessa Morais; Roberta Marcatti; Marlisson Rego; Emerson Araújo; Steven Witkin; Fabiola Villanova; Xutao Deng; Ester Sabino; Eric Delwart; Élcio Leal; Antonio da Costa. 2021. "Adaptive Evolution of New Variants of Dengue Virus Serotype 1 Genotype V Circulating in the Brazilian Amazon." Viruses 13, no. 4: 689.
Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.
Endrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses 2021, 13, 595 .
AMA StyleEndrya Do Socorro Fôro Ramos, Ulisses Rosa, Geovani De Oliveira Ribeiro, Fabiola Villanova, Flávio de Pádua Milagres, Rafael Brustulin, Vanessa Dos Santos Morais, Mayara Bertanhe, Roberta Marcatti, Emerson Araújo, Steven Witkin, Eric Delwart, Adriana Luchs, Antonio da Costa, Élcio Leal. High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis. Viruses. 2021; 13 (4):595.
Chicago/Turabian StyleEndrya Do Socorro Fôro Ramos; Ulisses Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio de Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Mayara Bertanhe; Roberta Marcatti; Emerson Araújo; Steven Witkin; Eric Delwart; Adriana Luchs; Antonio da Costa; Élcio Leal. 2021. "High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis." Viruses 13, no. 4: 595.
Husavirus (HuV) is an unclassified virus of the order Picornavirales that has already been identified worldwide in various locations. The genetic, epidemiological, and pathogenic characteristics are, however, little understood. In children with acute gastroenteritis, this study used next-generation sequencing to recognize unknown sources of viruses. In particular, 251 fecal samples obtained from individuals were sequenced in southern, northeastern, and northern Brazil. all samples were also analyzed using culture methods and parasitological tests to classify other enteric pathogens such as bacteria, parasites, and viruses. 1.9% of the samples tested positive for HuV, for a total of 5 positive children, with a mean age of 2 year, with three males and two females. Detailed molecular characterization of full genomes showed that Brazilian HuVs’ nucleotide divergence is less than 11%. The genetic gap between Brazilian sequences and the closest HuV reported previously, on the other hand, is 18%. The study showed that Brazilian sequences are closely related to the HuV defined in Viet Nam in 2013, further characterization based on phylogenetics. At least two divergent clades of HuV in South America were also seen in the phylogenetic study.
Endrya Do Socorro Fôro Ramos; Ulisses Alves Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio Augusto De Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Emerson Luiz Lima Araújo; Ramendra Pati Pandey; V. Samuel Raj; Ester Cerdeira Sabino; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Antonio Charlys da Costa. Multiple clades of Husavirus in South America revealed by next generation sequencing. PLOS ONE 2021, 16, e0248486 .
AMA StyleEndrya Do Socorro Fôro Ramos, Ulisses Alves Rosa, Geovani De Oliveira Ribeiro, Fabiola Villanova, Flávio Augusto De Pádua Milagres, Rafael Brustulin, Vanessa Dos Santos Morais, Emerson Luiz Lima Araújo, Ramendra Pati Pandey, V. Samuel Raj, Ester Cerdeira Sabino, Xutao Deng, Eric Delwart, Adriana Luchs, Élcio Leal, Antonio Charlys da Costa. Multiple clades of Husavirus in South America revealed by next generation sequencing. PLOS ONE. 2021; 16 (3):e0248486.
Chicago/Turabian StyleEndrya Do Socorro Fôro Ramos; Ulisses Alves Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Flávio Augusto De Pádua Milagres; Rafael Brustulin; Vanessa Dos Santos Morais; Emerson Luiz Lima Araújo; Ramendra Pati Pandey; V. Samuel Raj; Ester Cerdeira Sabino; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Antonio Charlys da Costa. 2021. "Multiple clades of Husavirus in South America revealed by next generation sequencing." PLOS ONE 16, no. 3: e0248486.
Classical insect-flaviviruses (cISFVs) and dual host-related insect-specific flavivirus (dISFV) are within the major group of insect-specific flavivirus. Remarkably dISFV are evolutionarily related to some of the pathogenic flavivirus, such as Zika and dengue viruses. The Evolutionary relatedness of dISFV to flavivirus allowed us to investigate the evolutionary principle of host adaptation. Additionally, dISFV can be used for the development of flavivirus vaccines and to explore underlying principles of mammalian pathogenicity. Here we describe the genetic characterization of a novel putative dISFV, termed Guapiaçu virus (GUAPV). Distinct strains of GUAPV were isolated from pools of Aedes terrens and Aedes scapularis mosquitoes. Additionally, we also detected viral GUAPV RNA in a plasma sample of an individual febrile from the Amazon region (North of Brazil). Although GUAPV did not replicate in tested mammalian cells, 3′UTR secondary structures duplication and codon usage index were similar to pathogenic flavivirus.
Geovani De Oliveira Ribeiro; Antonio Charlys da Costa; Danielle Elise Gill; Edcelha Soares D’Athaide Ribeiro; Marlisson Octavio da S. Rego; Fred Julio Costa Monteiro; Fabiola Villanova; Juliana Silva Nogueira; Adriana Yurika Maeda; Renato Pereira de Souza; Roozbeh Tahmasebi; Vanessa S. Morais; Ramendra Pati Pandey; V. Samuel Raj; Sirle Abdo Salloum Scandar; Fernanda Gisele da Silva Vasami; Leandro Guaraglia D’Agostino; Paulo César Maiorka; Xutao Deng; Maurício Lacerda Nogueira; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal; Mariana Sequetin Cunha. Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil. Scientific Reports 2021, 11, 1 -13.
AMA StyleGeovani De Oliveira Ribeiro, Antonio Charlys da Costa, Danielle Elise Gill, Edcelha Soares D’Athaide Ribeiro, Marlisson Octavio da S. Rego, Fred Julio Costa Monteiro, Fabiola Villanova, Juliana Silva Nogueira, Adriana Yurika Maeda, Renato Pereira de Souza, Roozbeh Tahmasebi, Vanessa S. Morais, Ramendra Pati Pandey, V. Samuel Raj, Sirle Abdo Salloum Scandar, Fernanda Gisele da Silva Vasami, Leandro Guaraglia D’Agostino, Paulo César Maiorka, Xutao Deng, Maurício Lacerda Nogueira, Ester Cerdeira Sabino, Eric Delwart, Élcio Leal, Mariana Sequetin Cunha. Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil. Scientific Reports. 2021; 11 (1):1-13.
Chicago/Turabian StyleGeovani De Oliveira Ribeiro; Antonio Charlys da Costa; Danielle Elise Gill; Edcelha Soares D’Athaide Ribeiro; Marlisson Octavio da S. Rego; Fred Julio Costa Monteiro; Fabiola Villanova; Juliana Silva Nogueira; Adriana Yurika Maeda; Renato Pereira de Souza; Roozbeh Tahmasebi; Vanessa S. Morais; Ramendra Pati Pandey; V. Samuel Raj; Sirle Abdo Salloum Scandar; Fernanda Gisele da Silva Vasami; Leandro Guaraglia D’Agostino; Paulo César Maiorka; Xutao Deng; Maurício Lacerda Nogueira; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal; Mariana Sequetin Cunha. 2021. "Guapiaçu virus, a new insect-specific flavivirus isolated from two species of Aedes mosquitoes from Brazil." Scientific Reports 11, no. 1: 1-13.
From 2010-2016, a total of 251 stool samples were screened for norovirus using next-generation sequencing (NGS) followed by phylogenetic analysis to investigate the genotypic diversity of noroviruses in rural and low-income urban areas in northern Brazil. Norovirus infection was detected in 19.9% (50/251) of the samples. Eight different genotypes were identified: GII.4_Sydney[P31] (64%, 32/50), GII.6[P7] (14%, 7/50), GII.17[P17] (6%, 3/50), GII.1[P33] (6%, 3/50), GII.3[P16] (4%, 2/50), GII.2[P16] (2%, 1/50), GII.2[P2] (2%, 1/50), and GII.4_New Orleans[P4] (2%, 1/50). Distinct GII.6[P7] variants were recognized, indicating the presence of different co-circulating strains. Elucidating norovirus genetic diversity will improve our understanding of their potential health burden, in particular for the GII.4_Sydney[P31] variant.
Rory J. Tinker; Antonio Charlys da Costa; Roozbeh Tahmasebi; Flavio Augusto De Pádua Milagres; Vanessa Dos Santos Morais; Ramendra Pati Pandey; Alexis José-Abrego; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Mariana Sequetin Cunha; Emerson Luiz Lima Araújo; Mariela Martínez Gómez; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Elcio Leal; Adriana Luchs. Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of Virology 2021, 166, 905 -913.
AMA StyleRory J. Tinker, Antonio Charlys da Costa, Roozbeh Tahmasebi, Flavio Augusto De Pádua Milagres, Vanessa Dos Santos Morais, Ramendra Pati Pandey, Alexis José-Abrego, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Mariana Sequetin Cunha, Emerson Luiz Lima Araújo, Mariela Martínez Gómez, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Elcio Leal, Adriana Luchs. Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil. Archives of Virology. 2021; 166 (3):905-913.
Chicago/Turabian StyleRory J. Tinker; Antonio Charlys da Costa; Roozbeh Tahmasebi; Flavio Augusto De Pádua Milagres; Vanessa Dos Santos Morais; Ramendra Pati Pandey; Alexis José-Abrego; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Mariana Sequetin Cunha; Emerson Luiz Lima Araújo; Mariela Martínez Gómez; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Elcio Leal; Adriana Luchs. 2021. "Norovirus strains in patients with acute gastroenteritis in rural and low-income urban areas in northern Brazil." Archives of Virology 166, no. 3: 905-913.
Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.
Roozbeh Tahmasebi; Adriana Luchs; Kaelan Tardy; Philip Michael Hefford; Rory J. Tinker; Owrang Eilami; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Vanessa Dos Santos Morais; Carlos Henrique Valente Moreira; Renata Buccheri; Emerson Luiz Lima Araújo; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal; Antonio Charlys Da Costa. Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics. Journal of General Virology 2020, 101, 1280 -1288.
AMA StyleRoozbeh Tahmasebi, Adriana Luchs, Kaelan Tardy, Philip Michael Hefford, Rory J. Tinker, Owrang Eilami, Flavio Augusto De Padua Milagres, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Vanessa Dos Santos Morais, Carlos Henrique Valente Moreira, Renata Buccheri, Emerson Luiz Lima Araújo, Fabiola Villanova, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Élcio Leal, Antonio Charlys Da Costa. Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics. Journal of General Virology. 2020; 101 (12):1280-1288.
Chicago/Turabian StyleRoozbeh Tahmasebi; Adriana Luchs; Kaelan Tardy; Philip Michael Hefford; Rory J. Tinker; Owrang Eilami; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Vanessa Dos Santos Morais; Carlos Henrique Valente Moreira; Renata Buccheri; Emerson Luiz Lima Araújo; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal; Antonio Charlys Da Costa. 2020. "Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics." Journal of General Virology 101, no. 12: 1280-1288.
Viruses are the most diverse and abundant microorganisms on earth, highly adaptive to a wide range of hosts. Viral diversity within invertebrate hosts has gained notoriety in recent years in public health as several such viruses have been of medical importance. Aedes aegypti serves as a vector for several viruses that have caused epidemics within the last year throughout Brazil; including Dengue, Zika and Chikungunya. This study aimed to identify new viral agents within Aedes aegypti mosquito in a city of the Amazonian region, where it is highly endemic. Metagenomic investigation was performed on 60 mosquito pools and viral RNA sequences present in their microbiota were characterized using genomic and phylogenetic tools. In total, we identified five putative novel virus species related to the Sobemovirus genus, Iflavirus genus and Permutatetraviridae family. These findings indicate a diverse taxonomy of viruses present in the mosquito microbiota of the Amazon, the region with the greatest invertebrate diversity in the world.
Geovani De Oliveira Ribeiro; Vanessa S. Morais; Fred Julio Costa Monteiro; Edcelha Soares D’Athaide Ribeiro; Marlisson Octavio Da S Rego; Raimundo Nonato Picanço Souto; Fabiola Villanova; Roozbeh Tahmasebi; Philip Michael Hefford; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Licia Natal Fernandes; Antonio Charlys Da Costa; Élcio Leal. Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species. Viruses 2020, 12, 866 .
AMA StyleGeovani De Oliveira Ribeiro, Vanessa S. Morais, Fred Julio Costa Monteiro, Edcelha Soares D’Athaide Ribeiro, Marlisson Octavio Da S Rego, Raimundo Nonato Picanço Souto, Fabiola Villanova, Roozbeh Tahmasebi, Philip Michael Hefford, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Licia Natal Fernandes, Antonio Charlys Da Costa, Élcio Leal. Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species. Viruses. 2020; 12 (8):866.
Chicago/Turabian StyleGeovani De Oliveira Ribeiro; Vanessa S. Morais; Fred Julio Costa Monteiro; Edcelha Soares D’Athaide Ribeiro; Marlisson Octavio Da S Rego; Raimundo Nonato Picanço Souto; Fabiola Villanova; Roozbeh Tahmasebi; Philip Michael Hefford; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Licia Natal Fernandes; Antonio Charlys Da Costa; Élcio Leal. 2020. "Aedes aegypti from Amazon Basin Harbor High Diversity of Novel Viral Species." Viruses 12, no. 8: 866.
Human Adenovirus species C (HAdV-C) is the most common etiologic agent of respiratory disease. In the present study, we characterized the nearly full-length genome of one potential new HAdV-C recombinant strain constituted by Penton and Fiber proteins belonging to type 89 and a chimeric Hexon protein of types 1 and 89. By using viral metagenomics techniques, we screened out, in the states of Tocantins and Pará, Northern and North regions of Brazil, from 2010 to 2016, 251 fecal samples of children between 0.5 to 2.5 years old. These children were presenting acute diarrhea not associated with common pathogens (i.e., rotavirus, norovirus). We identified two HAdV-C strains in two distinct patients. Phylogenetic analysis performed using all complete genomes available at GenBank database indicated that one strain (HAdV-C BR-245) belonged to type 1. The phylogenetic analysis also indicated that the second strain (HAdV-C BR-211) was located at the base of the clade formed by the newly HAdV-C strains type 89. Recombination analysis revealed that strain HAdV-C BR-211 is a chimera in which the variable regions of Hexon gene combined HAdV-C1 and HAdV-C89 sequences. Therefore, HAdV-C BR-211 strain possesses a genomic backbone of type HAdV-C89 and a unique insertion of HAdV-C1 in the Hexon sequence. Recombination may play an important driving force in HAdV-C diversity and evolution. Studies employing complete genomic sequencing on circulating HAdV-C strains in Brazil are needed to understand the clinical significance of the presented data.
Roozbeh Tahmasebi; Antonio Charlys Da Costa; Kaelan Tardy; Rory J. Tinker; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Rogério Togisaki Das Chagas; Cassia Vitória De Deus Alves Soares; Aripuana Sakurada Aranha Watanabe; Cecilia Salete Alencar; Fabiola Villanova; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Ester Cerdeira Sabino. Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil. Viruses 2020, 12, 508 .
AMA StyleRoozbeh Tahmasebi, Antonio Charlys Da Costa, Kaelan Tardy, Rory J. Tinker, Flavio Augusto De Padua Milagres, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Rogério Togisaki Das Chagas, Cassia Vitória De Deus Alves Soares, Aripuana Sakurada Aranha Watanabe, Cecilia Salete Alencar, Fabiola Villanova, Xutao Deng, Eric Delwart, Adriana Luchs, Élcio Leal, Ester Cerdeira Sabino. Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil. Viruses. 2020; 12 (5):508.
Chicago/Turabian StyleRoozbeh Tahmasebi; Antonio Charlys Da Costa; Kaelan Tardy; Rory J. Tinker; Flavio Augusto De Padua Milagres; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Rogério Togisaki Das Chagas; Cassia Vitória De Deus Alves Soares; Aripuana Sakurada Aranha Watanabe; Cecilia Salete Alencar; Fabiola Villanova; Xutao Deng; Eric Delwart; Adriana Luchs; Élcio Leal; Ester Cerdeira Sabino. 2020. "Genomic Analyses of Potential Novel Recombinant Human Adenovirus C in Brazil." Viruses 12, no. 5: 508.
Plasma from patients with dengue-like symptoms was collected in 2013 to 2016 from the Brazilian states of Tocantins and Amapa. 781 samples testing negative for IgM against Dengue, Zika, and Chikungunya viruses and for flaviviruses, alphaviruses and enteroviruses RNA using RT-PCRs were analyzed using viral metagenomics. Viral particles-associated nucleic acids were enriched, randomly amplified, and deep sequenced in 102 mini-pools generating over 2 billion reads. Sequence data was analyzed for the presence of known and novel eukaryotic viral reads. Anelloviruses were detected in 80%, human pegivirus 1 in 19%, and parvovirus B19 in 17% of plasma pools. HIV and enteroviruses were detected in two pools each. Previously uncharacterized viral genomes were also identified, and their presence in single plasma samples confirmed by PCR. Chapparvovirus and ambidensovirus genomes, both in the Parvoviridae family, were partially characterized showing 33% and 34% identity in their NS1 sequences to their closest relative. Molecular surveillance using pre-existing plasma from febrile patients provides a readily scalable approach for the detection of novel, potentially emerging, viruses.
Elizabeth Fahsbender; Antonio Charlys Da-Costa; Danielle Elise Gill; Flavio Augusto De Padua Milagres; Rafael Brustulin; Fred Julio Costa Monteiro; Marlisson Octavio Da Silva Rego; Edcelha Soares D’Athaide Ribeiro; Ester Cerdeira Sabino; Eric Delwart. Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus. PLoS ONE 2020, 15, e0229993 .
AMA StyleElizabeth Fahsbender, Antonio Charlys Da-Costa, Danielle Elise Gill, Flavio Augusto De Padua Milagres, Rafael Brustulin, Fred Julio Costa Monteiro, Marlisson Octavio Da Silva Rego, Edcelha Soares D’Athaide Ribeiro, Ester Cerdeira Sabino, Eric Delwart. Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus. PLoS ONE. 2020; 15 (3):e0229993.
Chicago/Turabian StyleElizabeth Fahsbender; Antonio Charlys Da-Costa; Danielle Elise Gill; Flavio Augusto De Padua Milagres; Rafael Brustulin; Fred Julio Costa Monteiro; Marlisson Octavio Da Silva Rego; Edcelha Soares D’Athaide Ribeiro; Ester Cerdeira Sabino; Eric Delwart. 2020. "Plasma virome of 781 Brazilians with unexplained symptoms of arbovirus infection include a novel parvovirus and densovirus." PLoS ONE 15, no. 3: e0229993.
Aedes aegypti is associated with epidemic diseases in Brazil, such as urban yellow fever, dengue, and more recently, chikungunya and Zika viruses infections. More information about Ae. aegypti infestation is fundamental to virological surveillance in order to ensure the effectiveness of control measures in use. Thus, the present study aims to identify and compare infestation and infectivity of Ae. aegypti females in Macapa city, Amapa State (Amazon region), Brazil, between the epidemiological weeks 2017/02 and 2018/20. A total number of 303 Ae. aegypti females were collected at 21 fixed collection points, 171 at the 10 collection points in the Marabaixo neighborhood and 132 at the 11 collection points in the Central neighborhood. Among the collected samples, only two were positive for dengue virus, with a 2.08% (2/96 pools) infectivity rate for Marabaixo. The difference between the medians of Ae. aegypti females captured in Central and Marabaixo sites was not statistically significant. The findings indicate similar mosquito infestation levels between the neighborhoods, and a low-level of mosquito infectivity, although dengue virus was found only in Marabaixo. Virological surveillance of Ae. aegypti was important to identify sites of infection and determine possible routes of transmission to enable health surveillance teams to adopt preventive strategies where infected mosquitoes are present and act faster.
Fred Júlio Costa Monteiro; Fábio Rodrigo Paixão Mourão; Edicelha Soares D’Athaide Ribeiro; Marlisson Octávio Da Silva Rêgo; Pablo Abdon Da Costa Frances; Raimundo Nonato Picanço Souto; Marlucia Dos Santos Façanha; Roozbeh Tahmasebi; Antônio Charlys Da Costa. Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil. Revista do Instituto de Medicina Tropical de São Paulo 2020, 62, e10 .
AMA StyleFred Júlio Costa Monteiro, Fábio Rodrigo Paixão Mourão, Edicelha Soares D’Athaide Ribeiro, Marlisson Octávio Da Silva Rêgo, Pablo Abdon Da Costa Frances, Raimundo Nonato Picanço Souto, Marlucia Dos Santos Façanha, Roozbeh Tahmasebi, Antônio Charlys Da Costa. Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil. Revista do Instituto de Medicina Tropical de São Paulo. 2020; 62 ():e10.
Chicago/Turabian StyleFred Júlio Costa Monteiro; Fábio Rodrigo Paixão Mourão; Edicelha Soares D’Athaide Ribeiro; Marlisson Octávio Da Silva Rêgo; Pablo Abdon Da Costa Frances; Raimundo Nonato Picanço Souto; Marlucia Dos Santos Façanha; Roozbeh Tahmasebi; Antônio Charlys Da Costa. 2020. "Prevalence of dengue, Zika and chikungunya viruses in Aedes (Stegomyia) aegypti (Diptera: Culicidae) in a medium-sized city, Amazon, Brazil." Revista do Instituto de Medicina Tropical de São Paulo 62, no. : e10.
Diarrhea remains one of the most common causes of deaths in children. Although many studies have investigated the prevalence of enteric pathogens around the globe some diarrheal episodes remain unexplained. It is possible that some yet-unidentified viral agents could be related to these cases of gastroenteritis. By using viral metagenomics techniques, we screened 251 fecal samples of children between 0.5 to 2.5-year-old with acute diarrhea not associated with common pathogens. These children live in rural areas and have different levels of contact with animals such as pigs, cows and bats. Here we report a complete genome of one mammalian orthoreovirus (MRV) type 3, denoted TO-151/BR, detected in a female child in the state of Tocantins (north of Brazil). Brazilian TO-151/BR strain was classified as MRV-3 based on S1 phylogeny and was closely related to porcine Asian strains. Phylogenetic analyses showed that other segments were more similar to MRV-3s of different geographic locations and hosts, including human and bats, highlighting genome reassortment and lack of host-specific barriers. This is the first report of MRV-3 in South America and a hypothesis of a silent long-term circulation of this virus in Brazil has been raised.
Ulisses Alves Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Adriana Luchs; Flavio Milagres; Shirley Vasconcelos Komninakis; Roozbeh Tahmasebi; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Rory J Tinker; Ramendra Pati Pandey; V. Samuel Raj; Ester Cerdeira Sabino; Xutao Deng; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil. Scientific Reports 2019, 9, 1 -7.
AMA StyleUlisses Alves Rosa, Geovani De Oliveira Ribeiro, Fabiola Villanova, Adriana Luchs, Flavio Milagres, Shirley Vasconcelos Komninakis, Roozbeh Tahmasebi, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Rory J Tinker, Ramendra Pati Pandey, V. Samuel Raj, Ester Cerdeira Sabino, Xutao Deng, Eric Delwart, Antonio Charlys Da Costa, Élcio Leal. First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil. Scientific Reports. 2019; 9 (1):1-7.
Chicago/Turabian StyleUlisses Alves Rosa; Geovani De Oliveira Ribeiro; Fabiola Villanova; Adriana Luchs; Flavio Milagres; Shirley Vasconcelos Komninakis; Roozbeh Tahmasebi; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Rory J Tinker; Ramendra Pati Pandey; V. Samuel Raj; Ester Cerdeira Sabino; Xutao Deng; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. 2019. "First identification of mammalian orthoreovirus type 3 by gut virome analysis in diarrheic child in Brazil." Scientific Reports 9, no. 1: 1-7.
We characterized the 24 nearly full-length genomes of human parechoviruses (PeV) from children in the north of Brazil. The initial phylogenetic analysis indicated that 17 strains belonged to genotype 1, 5 to genotype 4, and 1 to genotype 17. A more detailed analysis revealed a high frequency of recombinant strains (58%): A total of 14 of our PeV-As were chimeric, with four distinct recombination patterns identified. Five strains were composed of genotypes 1 and 5 (Rec1/5); five strains shared a complex mosaic pattern formed by genotypes 4, 5, and 17 (Rec4/17/5); two strains were composed of genotypes 1 and 17 (Rec1/17); and two strains were composed of genotype 1 and an undetermined strain (Rec1/und). Coalescent analysis based on the Vp1 gene, which is free of recombination, indicated that the recombinant strains most likely arose in this region approximately 30 years ago. They are present in high frequencies and are circulating in different small and isolated cities in the state of Tocantins. Further studies will be needed to establish whether the detected recombinant strains have been replacing parental strains or if they are co-circulating in distinct frequencies in Tocantins.
Élcio Leal; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cássia Vitória De Deus Alves Soares; Fabiola Villanova; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antônio Charlys Da Costa. Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil. Viruses 2019, 11, 488 .
AMA StyleÉlcio Leal, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cássia Vitória De Deus Alves Soares, Fabiola Villanova, Steven S. Witkin, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Antônio Charlys Da Costa. Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil. Viruses. 2019; 11 (6):488.
Chicago/Turabian StyleÉlcio Leal; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cássia Vitória De Deus Alves Soares; Fabiola Villanova; Steven S. Witkin; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antônio Charlys Da Costa. 2019. "Recombinant Strains of Human Parechovirus in Rural Areas in the North of Brazil." Viruses 11, no. 6: 488.
Beginning in late 2016 Brazil faced the worst outbreak of Yellow Fever in recent decades, mainly located in southeastern rural regions of the country. In the present study we characterize the Yellow Fever Virus (YFV) associated with this outbreak in São Paulo State, Brazil. Blood or tissues collected from 430 dead monkeys and 1030 pools containing a total of 5,518 mosquitoes were tested for YFV by quantitative RT-PCR, immunohistochemistry (IHC) and indirect immunofluorescence. A total of 67 monkeys were YFV-positive and 3 pools yielded YFV following culture in a C6/36 cell line. Analysis of five nearly full length genomes of YFV from collected samples was consistent with evidence that the virus associated with the São Paulo outbreak originated in Minas Gerais. The phylogenetic analysis also showed that strains involved in the 2016–2017 outbreak in distinct Brazilian states (i.e., Minas Gerais, Rio de Janeiro, Espirito Santo) intermingled in maximum-likelihood and Bayesian trees. Conversely, the strains detected in São Paulo formed a monophyletic cluster, suggesting that they were local-adapted. The finding of YFV by RT-PCR in five Callithrix monkeys who were all YFV-negative by histopathology or immunohistochemistry suggests that this YFV lineage circulating in Sao Paulo is associated with different outcomes in Callithrix when compared to other monkeys.
Mariana Sequetin Cunha; Antonio Charlys Da Costa; Natália Coelho Couto De Azevedo Fernandes; Juliana Mariotti Guerra; Fabiana Cristina Pereira Dos Santos; Juliana Silva Nogueira; Leandro Guariglia D’Agostino; Shirley Vasconcelos Komninakis; Steven S. Witkin; Rodrigo Albergaria Ressio; Adriana Yurika Maeda; Fernanda Gisele Silva Vasami; Ursula Mitsue Abreu Kaigawa; Laís Sampaio De Azevedo; Paloma Alana De Souza Facioli; Fernando Luiz De Lima Macedo; Ester Cerdeira Sabino; Élcio Leal; Renato Pereira de Souza. Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016–2017). Scientific Reports 2019, 9, 1 -13.
AMA StyleMariana Sequetin Cunha, Antonio Charlys Da Costa, Natália Coelho Couto De Azevedo Fernandes, Juliana Mariotti Guerra, Fabiana Cristina Pereira Dos Santos, Juliana Silva Nogueira, Leandro Guariglia D’Agostino, Shirley Vasconcelos Komninakis, Steven S. Witkin, Rodrigo Albergaria Ressio, Adriana Yurika Maeda, Fernanda Gisele Silva Vasami, Ursula Mitsue Abreu Kaigawa, Laís Sampaio De Azevedo, Paloma Alana De Souza Facioli, Fernando Luiz De Lima Macedo, Ester Cerdeira Sabino, Élcio Leal, Renato Pereira de Souza. Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016–2017). Scientific Reports. 2019; 9 (1):1-13.
Chicago/Turabian StyleMariana Sequetin Cunha; Antonio Charlys Da Costa; Natália Coelho Couto De Azevedo Fernandes; Juliana Mariotti Guerra; Fabiana Cristina Pereira Dos Santos; Juliana Silva Nogueira; Leandro Guariglia D’Agostino; Shirley Vasconcelos Komninakis; Steven S. Witkin; Rodrigo Albergaria Ressio; Adriana Yurika Maeda; Fernanda Gisele Silva Vasami; Ursula Mitsue Abreu Kaigawa; Laís Sampaio De Azevedo; Paloma Alana De Souza Facioli; Fernando Luiz De Lima Macedo; Ester Cerdeira Sabino; Élcio Leal; Renato Pereira de Souza. 2019. "Epizootics due to Yellow Fever Virus in São Paulo State, Brazil: viral dissemination to new areas (2016–2017)." Scientific Reports 9, no. 1: 1-13.
The nearly complete genome sequences of two Cucumis melo endornavirus (CmEV) strains were obtained using deep sequencing while investigating fecal samples for the presence of gastroenteritis viruses. The Brazilian CmEV BRA/TO-23 (aa positions 116-5027) and BRA/TO-74 (aa positions 26-5057) strains were nearly identical to the reference CmEV CL-01 (USA) and SJ1 (South Korea) strains, showing 97% and 98% of nucleotide and amino acid identity, respectively. Endornaviruses are not known to be associated with human disease and their presence may simply reflect recent dietary consumption. Metagenomic analyses offered an opportunity to identify for the first time in Brazil a newly described endornavirus species.
Antonio Charlys Da Costa; Elcio Leal; Danielle Gill; Flavio Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Adriana Luchs. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes 2019, 55, 332 -338.
AMA StyleAntonio Charlys Da Costa, Elcio Leal, Danielle Gill, Flavio Milagres, Shirley Vasconcelos Komninakis, Rafael Brustulin, Maria Da Aparecida Rodrigues Teles, Márcia Cristina Alves Brito Sayão Lobato, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Adriana Luchs. Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil. Virus Genes. 2019; 55 (3):332-338.
Chicago/Turabian StyleAntonio Charlys Da Costa; Elcio Leal; Danielle Gill; Flavio Milagres; Shirley Vasconcelos Komninakis; Rafael Brustulin; Maria Da Aparecida Rodrigues Teles; Márcia Cristina Alves Brito Sayão Lobato; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Adriana Luchs. 2019. "Discovery of Cucumis melo endornavirus by deep sequencing of human stool samples in Brazil." Virus Genes 55, no. 3: 332-338.
Emergence of DS-1-like-G1P[8] rotavirus in Asia have been recently reported. We report for the first time the detection and the whole genome phylogenetic analysis of DS-1-like-G1P[8] strains in America. From 2013 to 2017, a total of 4226 fecal samples were screened for rotavirus by ELISA, PAGE, RT-PCR and sequencing. G1P[8] represented 3.7% (30/800) of all rotavirus-positive samples. DS-1-like-G1P[8] comprised 1.6% (13/800) detected exclusively in 2013, and Wa-like-G1P[8] comprised 2.1% (17/800) detected from 2013 to 2015. Whole genome sequencing confirmed the DS-1-like backbone I2-R2-C2-M2-A2-N2-T2-E2-H2. All genome segments of the Brazilian DS-1-like-G1P[8] strains clustered with those of Asian strains, and apart from African DS-1-like-G1P[8] strains. In addition, Brazilian DS-1-like-G1P[8] reassortants distantly clustered with DS-1-like backbone strains simultaneously circulating in the country, suggesting that the Brazilian DS-1-like-G1P[8] strains are likely imported from Asia. Two distinct NSP4 E2 genotype lineages were also identified, indicating the existence of a co-circulating pool of different DS-1-like G1P[8] strains. Surveillance systems must be developed to examine if RVA vaccines are still effective for the prevention against unusual DS-1-like-G1P[8] strains.
Adriana Luchs; Antonio Charlys Da Costa; Audrey Cilli; Shirley Cavalcante Vasconcelos Komninakis; Rita Carmona; Simone Guadagnucci Morillo; Ester Cerdeira Sabino; Maria Do Carmo Sampaio Tavares Timenetsky. First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013. Scientific Reports 2019, 9, 2210 .
AMA StyleAdriana Luchs, Antonio Charlys Da Costa, Audrey Cilli, Shirley Cavalcante Vasconcelos Komninakis, Rita Carmona, Simone Guadagnucci Morillo, Ester Cerdeira Sabino, Maria Do Carmo Sampaio Tavares Timenetsky. First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013. Scientific Reports. 2019; 9 (1):2210.
Chicago/Turabian StyleAdriana Luchs; Antonio Charlys Da Costa; Audrey Cilli; Shirley Cavalcante Vasconcelos Komninakis; Rita Carmona; Simone Guadagnucci Morillo; Ester Cerdeira Sabino; Maria Do Carmo Sampaio Tavares Timenetsky. 2019. "First Detection of DS-1-like G1P[8] Double-gene Reassortant Rotavirus Strains on The American Continent, Brazil, 2013." Scientific Reports 9, no. 1: 2210.
Advancements in next-generation sequencing and bioinformatics have expanded our knowledge of the diversity of viruses (pathogens and non-pathogens) harbored by mosquitoes. Hubei reo-like virus 7 (HRLV 7) was recently detected by the virome analysis of fecal samples from migratory birds in Australia. We now report the detection of RNA-dependent RNA polymerase sequences of HRLV 7 in pools of Aedes aegypti and Culex quinquefasciatus mosquitoes species from the Brazilian Amazon forest. Phylogenetic inferences indicated that all HRLV 7 strains fall within the same independent clade. In addition, HRLV 7 shared a close ancestral lineage with the Dinovernavirus genus of the Reoviridae family. Our findings indicate that HRLV 7 is present in two species of mosquitoes.
Geovani De Oliveira Ribeiro; Fred Julio Costa Monteiro; Marlisson Octavio Da S Rego; Edcelha Soares D’Athaide Ribeiro; Daniela Funayama De Castro; Marcos Montani Caseiro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Steven S. Witkin; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys Da Costa; Élcio Leal. Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil. Viruses 2019, 11, 147 .
AMA StyleGeovani De Oliveira Ribeiro, Fred Julio Costa Monteiro, Marlisson Octavio Da S Rego, Edcelha Soares D’Athaide Ribeiro, Daniela Funayama De Castro, Marcos Montani Caseiro, Robson Dos Santos Souza Marinho, Shirley Vasconcelos Komninakis, Steven S. Witkin, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Antonio Charlys Da Costa, Élcio Leal. Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil. Viruses. 2019; 11 (2):147.
Chicago/Turabian StyleGeovani De Oliveira Ribeiro; Fred Julio Costa Monteiro; Marlisson Octavio Da S Rego; Edcelha Soares D’Athaide Ribeiro; Daniela Funayama De Castro; Marcos Montani Caseiro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Steven S. Witkin; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Antonio Charlys Da Costa; Élcio Leal. 2019. "Detection of RNA-Dependent RNA Polymerase of Hubei Reo-Like Virus 7 by Next-Generation Sequencing in Aedes aegypti and Culex quinquefasciatus Mosquitoes from Brazil." Viruses 11, no. 2: 147.
Enterovirus B73 is a new member of the Enterovirus B species. First detected in the USA, it has been subsequently identified in China, India, Oman, and the Netherlands. In this study, we characterize the first B73 strain (named TO-127) to be detected in South America. TO-127 was obtained from a child with acute gastroenteritis living in a rural area in Northern Brazil. The subject was not infected with any known enteric pathogens such as norovirus, rotavirus, helminths, or enteric bacteria. Analysis of the nearly full-length TO-127 genome (6993 nt) indicated a 74–75% nucleotide similarity with EV-B73 strains from other countries. Evolutionary analysis suggests that B73 is endemic and widespread.
Geovani De Oliveira Ribeiro; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Steven S. Witkin; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. Detection and Characterization of Enterovirus B73 from a Child in Brazil. Viruses 2018, 11, 16 .
AMA StyleGeovani De Oliveira Ribeiro, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Steven S. Witkin, Fabiola Villanova, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Antonio Charlys Da Costa, Élcio Leal. Detection and Characterization of Enterovirus B73 from a Child in Brazil. Viruses. 2018; 11 (1):16.
Chicago/Turabian StyleGeovani De Oliveira Ribeiro; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Steven S. Witkin; Fabiola Villanova; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Antonio Charlys Da Costa; Élcio Leal. 2018. "Detection and Characterization of Enterovirus B73 from a Child in Brazil." Viruses 11, no. 1: 16.
Classical insect-specific flaviviruses (cISFs) have been widely detected in different countries in the last decades. Here, we characterize the near full-length genomes of two cISFs detected in mosquitoes collected in the city of Macapá, state of Amapá, Amazon region of Brazil. A total of 105 pools of female mosquitos were analyzed by next-generation sequencing (NGS). Comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (CFAV) and two of Culex flavivirus (CxFV). All sequences were obtained from pools of Culex sp., except for one sequence of CFAV detected in a pool of Aedes aegypti. Both CxFV strains are phylogenetically related to a strain isolated in 2012 in the Southeast region of Brazil. The CFAV strains are the first of this species to be identified in Brazil and one of them is highly divergent from other strains of CFAV that have been detected worldwide. In conclusion, CFAV and CxFV, circulate in mosquitoes in Brazil. One strain of CFAV is highly divergent from others previously described, suggesting that a novel strain of CFAV is present in this region.
Licia Natal Fernandes; Thaís De Moura Coletti; Fred Julio Costa Monteiro; Marlisson Octavio Da Silva Rego; Edcelha Soares D'Athaide Ribeiro; Geovani De Oliveira Ribeiro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Steven S. Witkin; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Élcio Leal; Antonio Charlys Da Costa. A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region. Viruses 2018, 10, 666 .
AMA StyleLicia Natal Fernandes, Thaís De Moura Coletti, Fred Julio Costa Monteiro, Marlisson Octavio Da Silva Rego, Edcelha Soares D'Athaide Ribeiro, Geovani De Oliveira Ribeiro, Robson Dos Santos Souza Marinho, Shirley Vasconcelos Komninakis, Steven S. Witkin, Xutao Deng, Eric Delwart, Ester Cerdeira Sabino, Élcio Leal, Antonio Charlys Da Costa. A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region. Viruses. 2018; 10 (12):666.
Chicago/Turabian StyleLicia Natal Fernandes; Thaís De Moura Coletti; Fred Julio Costa Monteiro; Marlisson Octavio Da Silva Rego; Edcelha Soares D'Athaide Ribeiro; Geovani De Oliveira Ribeiro; Robson Dos Santos Souza Marinho; Shirley Vasconcelos Komninakis; Steven S. Witkin; Xutao Deng; Eric Delwart; Ester Cerdeira Sabino; Élcio Leal; Antonio Charlys Da Costa. 2018. "A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region." Viruses 10, no. 12: 666.
Here we report the nearly full-length genome of a recombinant Saffold virus strain (SAFV-BR-193) isolated from a child with acute gastroenteritis. Evolutionary analysis performed using all available near-full length Saffold picornavirus genomes showed that the breakpoint found in the Brazilian strain (SAFV-BR-193) is indeed a recombination hotspot. Notably, this hotspot is located just one nucleotide after the ribosomal frameshift GGUUUUU motif in the SAFV genome. Empirical studies will be necessary to determine if this motif also affects the binding affinity of RNA-dependent RNA-polymerase (RdRp) and therefore increases the changes of RdRp swap between molecules during the synthesis of viral genomes.
Antônio Charlys Da Costa; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses 2018, 10, 520 .
AMA StyleAntônio Charlys Da Costa, Adriana Luchs, Flávio Augusto De Pádua Milagres, Shirley Vasconcelos Komninakis, Danielle Elise Gill, Márcia Cristina Alves Brito Sayão Lobato, Rafael Brustulin, Rogério Togisaki Das Chagas, Maria De Fátima Neves Dos Santos Abrão, Cassia Vitória De Deus Alves Soares, Xutao Deng, Ester Cerdeira Sabino, Eric Delwart, Élcio Leal. Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus. Viruses. 2018; 10 (10):520.
Chicago/Turabian StyleAntônio Charlys Da Costa; Adriana Luchs; Flávio Augusto De Pádua Milagres; Shirley Vasconcelos Komninakis; Danielle Elise Gill; Márcia Cristina Alves Brito Sayão Lobato; Rafael Brustulin; Rogério Togisaki Das Chagas; Maria De Fátima Neves Dos Santos Abrão; Cassia Vitória De Deus Alves Soares; Xutao Deng; Ester Cerdeira Sabino; Eric Delwart; Élcio Leal. 2018. "Recombination Located over 2A-2B Junction Ribosome Frameshifting Region of Saffold Cardiovirus." Viruses 10, no. 10: 520.