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Dr. Przemysław Łukasz Kowalczewski
Poznań University of Life Sciences

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0 Food Science
0 Functional Foods
0 Rheology
0 cytotoxic activity
0 bioactive compounds

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antioxidant activities
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Review
Published: 12 August 2021 in Molecules
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Different parts of a plant (seeds, fruits, flower, leaves, stem, and roots) contain numerous biologically active compounds called “phytoconstituents” that consist of phenolics, minerals, amino acids, and vitamins. The conventional techniques applied to extract these phytoconstituents have several drawbacks including poor performance, low yields, more solvent use, long processing time, and thermally degrading by-products. In contrast, modern and advanced extraction nonthermal technologies such as pulsed electric field (PEF) assist in easier and efficient identification, characterization, and analysis of bioactive ingredients. Other advantages of PEF include cost-efficacy, less time, and solvent consumption with improved yields. This review covers the applications of PEF to obtain bioactive components, essential oils, proteins, pectin, and other important materials from various parts of the plant. Numerous studies compiled in the current evaluation concluded PEF as the best solution to extract phytoconstituents used in the food and pharmaceutical industries. PEF-assisted extraction leads to a higher yield, utilizes less solvents and energy, and it saves a lot of time compared to traditional extraction methods. PEF extraction design should be safe and efficient enough to prevent the degradation of phytoconstituents and oils.

ACS Style

Muhammad Modassar A. N. Ranjha; Rabia Kanwal; Bakhtawar Shafique; Rai Naveed Arshad; Shafeeqa Irfan; Marek Kieliszek; Przemysław Łukasz Kowalczewski; Muhammad Irfan; Muhammad Zubair Khalid; Ume Roobab; Rana Muhammad Aadil. A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents. Molecules 2021, 26, 4893 .

AMA Style

Muhammad Modassar A. N. Ranjha, Rabia Kanwal, Bakhtawar Shafique, Rai Naveed Arshad, Shafeeqa Irfan, Marek Kieliszek, Przemysław Łukasz Kowalczewski, Muhammad Irfan, Muhammad Zubair Khalid, Ume Roobab, Rana Muhammad Aadil. A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents. Molecules. 2021; 26 (16):4893.

Chicago/Turabian Style

Muhammad Modassar A. N. Ranjha; Rabia Kanwal; Bakhtawar Shafique; Rai Naveed Arshad; Shafeeqa Irfan; Marek Kieliszek; Przemysław Łukasz Kowalczewski; Muhammad Irfan; Muhammad Zubair Khalid; Ume Roobab; Rana Muhammad Aadil. 2021. "A Critical Review on Pulsed Electric Field: A Novel Technology for the Extraction of Phytoconstituents." Molecules 26, no. 16: 4893.

Journal article
Published: 11 August 2021 in Plants
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Contamination of malting barley grain and malt with micromycetes sampled at various periods post-harvest (3rd, 6th, and 9th month of storage) and types of storage (storage silo and floor warehouse) was investigated. Each of these barley grain samples was malted. This article reports on the changes in the fungal microbiome composition and their overall count in barley grain and malt. From the surface-disinfected barley grain samples collected immediately after harvest, there were eight genera isolated, with a predominance of Alternaria. A small increase of isolated microfungi was detected in barley stored in silo for 3 and 6 months (from 142 isolates to 149) and decreased below the number of isolates in barley before storage (133 isolates). Fungal count during storage gradually decreased up to 9 month in barley stored in floor warehouse (from 142 isolates to 84). The initial total count of microscopic fungi in malt before storage was the highest (112 isolates) with 7 genera detected, compared to malts prepared from barley stored for longer time (54 isolates, 7 genera, 9th month of storage). Alternaria was the most abundant and frequent genus. Quantitative representation of the filamentous microscopic fungi was lower compared to yeasts especially in barley and malt prepared from barley stored at third month of storage in both type of storage. Yeasts were identified from all grain samples and malt samples with mass spectrometry. Most attention was given to the widely distributed fungus Penicillium, 79% of strains produced at least one mycotoxin detected under in vitro assays using the TLC method (97% of them produced griseofulvin, 94% CPA, 79% patulin, 14% roquefortin C, and penitrem A was produced by two screening strains under laboratory conditions). It is therefore important to monitor the microflora throughout the production cycle of “barley to beer”.

ACS Style

Soňa Felšöciová; Przemysław Łukasz Kowalczewski; Tomáš Krajčovič; Štefan Dráb; Miroslava Kačániová. Effect of Long-Term Storage on Mycobiota of Barley Grain and Malt. Plants 2021, 10, 1655 .

AMA Style

Soňa Felšöciová, Przemysław Łukasz Kowalczewski, Tomáš Krajčovič, Štefan Dráb, Miroslava Kačániová. Effect of Long-Term Storage on Mycobiota of Barley Grain and Malt. Plants. 2021; 10 (8):1655.

Chicago/Turabian Style

Soňa Felšöciová; Przemysław Łukasz Kowalczewski; Tomáš Krajčovič; Štefan Dráb; Miroslava Kačániová. 2021. "Effect of Long-Term Storage on Mycobiota of Barley Grain and Malt." Plants 10, no. 8: 1655.

Journal article
Published: 11 July 2021 in Plants
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The aim of this study was to analyze the chemical composition and biological and antibiofilm activity of the essential oil (EO) of Thymus serpyllum with the use of a MALDI-TOF MS Biotyper. The main compounds of the EO were thymol, 18.8%; carvacrol, 17.4%; o-cymene, 15.4%; and geraniol, 10.7%. It was found that free-radical scavenging activity was high. The highest antimicrobial activity was observed against Pseudomonas aeruginosa, Salmonella enteritidis, and biofilm-forming bacteria. The changes in the biofilm structure after T. serpyllum EO application confirmed the inhibitory action and the most pronounced effect was observed on Bacillus subtilis biofilm. The antifungal activity of the vapor phase was the most effective against Penicillium crustosum. T. serpyllum should be a suitable alternative to synthetic antioxidants as well as antimicrobials. The EO of T. serpyllum can be used in the vapor phase in the storage of root vegetables as well as a growth inhibitor of Penicillium on bread.

ACS Style

Lucia Galovičová; Petra Borotová; Veronika Valková; Nenad Vukovic; Milena Vukic; Margarita Terentjeva; Jana Štefániková; Hana Ďúranová; Przemysław Kowalczewski; Miroslava Kačániová. Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver. Plants 2021, 10, 1416 .

AMA Style

Lucia Galovičová, Petra Borotová, Veronika Valková, Nenad Vukovic, Milena Vukic, Margarita Terentjeva, Jana Štefániková, Hana Ďúranová, Przemysław Kowalczewski, Miroslava Kačániová. Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver. Plants. 2021; 10 (7):1416.

Chicago/Turabian Style

Lucia Galovičová; Petra Borotová; Veronika Valková; Nenad Vukovic; Milena Vukic; Margarita Terentjeva; Jana Štefániková; Hana Ďúranová; Przemysław Kowalczewski; Miroslava Kačániová. 2021. "Thymus serpyllum Essential Oil and Its Biological Activity as a Modern Food Preserver." Plants 10, no. 7: 1416.

Journal article
Published: 01 July 2021 in Polymers
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Starch is a widely known and used emulsion stabilizer. In order to improve its properties, various types of modifications are made that change its ability to emulsify and stabilize. This paper describes the analysis of the molecular dynamics of water using low-field nuclear magnetic resonance (LF NMR) in oil-in-water emulsions obtained with the use of physically or chemically modified potato starch. The analysis of changes in spin-spin and spin-lattice relaxation times depending on the temperature allowed the activation energy value of water molecules in the analyzed emulsions to be determined. It has been shown that the presence of starch influences the values of spin-lattice T1 and spin-spin T2 relaxation times, both in the water and the oil phase, and the observed changes largely depended on the type of starch modification. Both types of analyzed starches also differently influenced the energy of activation of rotational movements of water molecules. On the basis of the analyses carried out with the use of LF NMR, it can be concluded that physically modified starch acts not only as a stabilizer, but also as an emulsifier, while acetylated starch does not exhibit good emulsifying properties.

ACS Style

Zuzanna Małyszek; Jacek Lewandowicz; Joanna Le Thanh-Blicharz; Katarzyna Walkowiak; Przemysław Kowalczewski; Hanna Baranowska. Water Behavior of Emulsions Stabilized by Modified Potato Starch. Polymers 2021, 13, 2200 .

AMA Style

Zuzanna Małyszek, Jacek Lewandowicz, Joanna Le Thanh-Blicharz, Katarzyna Walkowiak, Przemysław Kowalczewski, Hanna Baranowska. Water Behavior of Emulsions Stabilized by Modified Potato Starch. Polymers. 2021; 13 (13):2200.

Chicago/Turabian Style

Zuzanna Małyszek; Jacek Lewandowicz; Joanna Le Thanh-Blicharz; Katarzyna Walkowiak; Przemysław Kowalczewski; Hanna Baranowska. 2021. "Water Behavior of Emulsions Stabilized by Modified Potato Starch." Polymers 13, no. 13: 2200.

Original paper
Published: 30 June 2021 in European Food Research and Technology
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Dried fruits are an excellent alternative to unhealthy snacks. Twelve commercially available dried fruits were selected: dates, raisins, prunes, Goji berry, chokeberry, rose hip, sea buckthorn, berberis, physalis, haritaki, noni and juniper. The nutritional value in terms of moisture, ash, protein, fat, carbohydrate, dietary fiber, energy value, mineral composition, antioxidant activity and tannins was compared. It is a novelty in the literature in relation to the particular analytes (e.g., minerals, tannins) and/or fruits (e.g., berberis, noni, haritaki). Especially rich in protein were Goji berry (13.3%), sea buckthorn (9.3%), noni (8.9%) and physalis (8.0%); in fat − sea buckthorn (11.2%); in dietary fiber (4.4–53.0%) − most of analyzed products. High antioxidant capacity was noticed for haritaki, berberis, rose hip, Goji berry, and physalis. An important source of minerals was 100 g of: noni (345 mg of Ca; 251 mg of Mg), rose hip (844 mg of Ca; 207 mg of Mg), juniper (564 mg of Ca), sea buckthorn (58 mg of Fe), berberis (24 mg of Fe) and haritaki (14 mg of Fe). The nutritionally attractive dried fruits have the potential for wider application in food formulations.

ACS Style

Iga Rybicka; Justyna Kiewlicz; Przemysław Łukasz Kowalczewski; Anna Gliszczyńska-Świgło. Selected dried fruits as a source of nutrients. European Food Research and Technology 2021, 1 -11.

AMA Style

Iga Rybicka, Justyna Kiewlicz, Przemysław Łukasz Kowalczewski, Anna Gliszczyńska-Świgło. Selected dried fruits as a source of nutrients. European Food Research and Technology. 2021; ():1-11.

Chicago/Turabian Style

Iga Rybicka; Justyna Kiewlicz; Przemysław Łukasz Kowalczewski; Anna Gliszczyńska-Świgło. 2021. "Selected dried fruits as a source of nutrients." European Food Research and Technology , no. : 1-11.

Journal article
Published: 29 May 2021 in International Journal of Molecular Sciences
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Today, agricultural productivity is essential to meet the needs of a growing population, and is also a key tool in coping with climate change. Innovative plant breeding technologies such as molecular markers, phenotyping, genotyping, the CRISPR/Cas method and next-generation sequencing can help agriculture meet the challenges of the 21st century more effectively. Therefore, the aim of the research was to identify single-nucleotide polymorphisms (SNPs) and SilicoDArT markers related to select morphological features determining the yield in maize. The plant material consisted of ninety-four inbred lines of maize of various origins. These lines were phenotyped under field conditions. A total of 14 morphological features was analyzed. The DArTseq method was chosen for genotyping because this technique reduces the complexity of the genome by restriction enzyme digestion. Subsequently, short fragment sequencing was used. The choice of a combination of restrictases allowed the isolation of highly informative low copy fragments of the genome. Thanks to this method, 90% of the obtained DArTseq markers are complementary to the unique sequences of the genome. All the observed features were normally distributed. Analysis of variance indicated that the main effect of lines was statistically significant (p< 0.001) for all 14 traits of study. Thanks to the DArTseq analysis with the use of next-generation sequencing (NGS) in the studied plant material, it was possible to identify 49,911 polymorphisms, of which 33,452 are SilicoDArT markers and the remaining 16,459 are SNP markers. Among those mentioned, two markers associated with four analyzed traits deserved special attention: SNP (4578734) and SilicoDArT (4778900). SNP marker 4578734 was associated with the following features: anthocyanin coloration of cob glumes, number of days from sowing to anthesis, number of days from sowing to silk emergence and anthocyanin coloration of internodes. SilicoDArT marker 4778900 was associated with the following features: number of days from sowing to anthesis, number of days from sowing to silk emergence, tassel: angle between the axis and lateral branches and plant height. Sequences with a length of 71 bp were used for physical mapping. The BLAST and EnsemblPlants databases were searched against the maize genome to identify the positions of both markers. Marker 4578734 was localized on chromosome 7, the closest gene was Zm00001d022467, approximately 55 Kb apart, encoding anthocyanidin 3-O-glucosyltransferase. Marker 4778900 was located on chromosome 7, at a distance of 45 Kb from the gene Zm00001d045261 encoding starch synthase I. The latter observation indicated that these flanking SilicoDArT and SNP markers were not in a state of linkage disequilibrium.

ACS Style

Agnieszka Tomkowiak; Jan Bocianowski; Julia Spychała; Joanna Grynia; Aleksandra Sobiech; Przemysław Kowalczewski. DArTseq-Based High-Throughput SilicoDArT and SNP Markers Applied for Association Mapping of Genes Related to Maize Morphology. International Journal of Molecular Sciences 2021, 22, 5840 .

AMA Style

Agnieszka Tomkowiak, Jan Bocianowski, Julia Spychała, Joanna Grynia, Aleksandra Sobiech, Przemysław Kowalczewski. DArTseq-Based High-Throughput SilicoDArT and SNP Markers Applied for Association Mapping of Genes Related to Maize Morphology. International Journal of Molecular Sciences. 2021; 22 (11):5840.

Chicago/Turabian Style

Agnieszka Tomkowiak; Jan Bocianowski; Julia Spychała; Joanna Grynia; Aleksandra Sobiech; Przemysław Kowalczewski. 2021. "DArTseq-Based High-Throughput SilicoDArT and SNP Markers Applied for Association Mapping of Genes Related to Maize Morphology." International Journal of Molecular Sciences 22, no. 11: 5840.

Journal article
Published: 23 February 2021 in Molecules
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Cricket powder, described in the literature as a source of nutrients, can be a valuable ingredient to supplement deficiencies in various food products. Work continues on the implementation of cricket powder in products that are widely consumed. The aim of this study was to obtain gluten-free bread with a superior nutritional profile by means of insect powder addition. Gluten-free breads enriched with 2%, 6%, and 10% of cricket (Acheta domesticus) powder were formulated and extensively characterized. The nutritional value, as well as antioxidant and β-glucuronidase activities, were assessed after simulated in vitro digestion. Addition of cricket powder significantly increased the nutritional value, both in terms of the protein content (exceeding two-, four-, and seven-fold the reference bread (RB), respectively) and above all mineral compounds. The most significant changes were observed for Cu, P, and Zn. A significant increase in the content of polyphenolic compounds and antioxidant activity in the enriched bread was also demonstrated; moreover, both values additionally increased after the digestion process. The total polyphenolic compounds content increased about five-fold from RB to bread with 10% CP (BCP10), and respectively about three-fold after digestion. Similarly, the total antioxidant capacity before digestion increased about four-fold, and after digestion about six-fold. The use of CP also reduced the undesirable activity of β-glucuronidase by 65.9% (RB vs. BCP10) in the small intestine, down to 78.9% in the large intestine. The influence of bread on the intestinal microflora was also evaluated, and no inhibitory effect on the growth of microflora was demonstrated, both beneficial (Bifidobacterium and Lactobacillus) and pathogenic (Enterococcus and Escherichia coli). Our results underscore the benefits of using cricket powder to increase the nutritional value and biological activity of gluten-free food products.

ACS Style

Przemysław Kowalczewski; Małgorzata Gumienna; Iga Rybicka; Barbara Górna; Paulina Sarbak; Krzysztof Dziedzic; Dominik Kmiecik. Nutritional Value and Biological Activity of Gluten-Free Bread Enriched with Cricket Powder. Molecules 2021, 26, 1184 .

AMA Style

Przemysław Kowalczewski, Małgorzata Gumienna, Iga Rybicka, Barbara Górna, Paulina Sarbak, Krzysztof Dziedzic, Dominik Kmiecik. Nutritional Value and Biological Activity of Gluten-Free Bread Enriched with Cricket Powder. Molecules. 2021; 26 (4):1184.

Chicago/Turabian Style

Przemysław Kowalczewski; Małgorzata Gumienna; Iga Rybicka; Barbara Górna; Paulina Sarbak; Krzysztof Dziedzic; Dominik Kmiecik. 2021. "Nutritional Value and Biological Activity of Gluten-Free Bread Enriched with Cricket Powder." Molecules 26, no. 4: 1184.

Journal article
Published: 06 February 2021 in Molecules
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The results of recently published studies indicate that potato juice is characterized by interesting biological activity that can be particularly useful in the case of gastrointestinal symptoms. Moreover, the studies also described the high nutritional value of its proteins. This article is a report on the impact of the enzymatic hydrolysis of proteins combined with membrane filtration. The obtained potato juice protein hydrolysate (PJPH) and its concentrate (cPJPH) were characterized in terms of their nutritional value and biological activity. The amino acid profile and scoring, the content of mineral compounds, and the antioxidant and in vitro cytotoxic activity were assessed. The study proved that the antioxidant activity of PJPH is higher than that of fresh potato juice, and the cytotoxicity against human gastric carcinoma cell line (Hs 746T), human colon cancer cell line (Caco-2), human colorectal adenocarcinoma cell line (HT-29), and human normal colon mucosa cell line (CCD 841 CoN) showed biological activity specifically targeted against cancer cells. Therefore, it can be concluded that the membrane filtration-assisted enzymatic hydrolysis of potato juice proteins may increase their biological activity and allow for potato juice to be used in the production of medicinal preparations.

ACS Style

Przemysław Kowalczewski; Anna Olejnik; Iga Rybicka; Magdalena Zielińska-Dawidziak; Wojciech Białas; Grażyna Lewandowicz. Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice. Molecules 2021, 26, 852 .

AMA Style

Przemysław Kowalczewski, Anna Olejnik, Iga Rybicka, Magdalena Zielińska-Dawidziak, Wojciech Białas, Grażyna Lewandowicz. Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice. Molecules. 2021; 26 (4):852.

Chicago/Turabian Style

Przemysław Kowalczewski; Anna Olejnik; Iga Rybicka; Magdalena Zielińska-Dawidziak; Wojciech Białas; Grażyna Lewandowicz. 2021. "Membrane Filtration-Assisted Enzymatic Hydrolysis Affects the Biological Activity of Potato Juice." Molecules 26, no. 4: 852.

Research article
Published: 14 January 2021 in Food Science and Technology International
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The paper presents the effect of replacing starch (at 2%, 6% and 10%) with cricket powder (CP) on the water behavior studied by the 1H NMR method, as well as the texture of gluten-free bread during 6-day storage. It was noticed that the bread crumb containing CP has lower water transport rate than the control bread crumb, while concluding that 2% CP stabilizes water transport throughout the entire staling time range. The NMR analyzes showed that the initial T21 values are the higher, the more starch has been replaced with the CP, however, after 6 days of storage, all tested samples are characterized by similar values of the T21 parameter. A decrease in long component of spin-spin relaxation time T22 during storage was also observed. It has been noted that the replacement of starch to 2% and 6% CP causes an increase in the molecular dynamics of water. The less starch present, the greater the potential for bulk molecules to move. The observed changes at the molecular level resulted in macroscopic changes in the texture of the bread. After analyzing the hardness parameter of the tested breads, it was found that on the day of baking, bread without the addition of CP had significantly higher values of this parameter than breads with CP. For the sample without CP, the highest increase in total hardness change (123.93%) was noted during storage, which indicates the fastest texture change process. Based on the results obtained, it can be concluded that the use of cricket powder to enrich gluten-free bread can not only improve the nutritional value, but also effectively delay the process of bread staling.

ACS Style

Przemysław Łukasz Kowalczewski; Katarzyna Walkowiak; Łukasz Masewicz; Krzysztof Smarzyński; Joanna Le Thanh-Blicharz; Miroslava Kačániová; Hanna Maria Baranowska. LF NMR spectroscopy analysis of water dynamics and texture of Gluten-Free bread with cricket powder during storage. Food Science and Technology International 2021, 1 .

AMA Style

Przemysław Łukasz Kowalczewski, Katarzyna Walkowiak, Łukasz Masewicz, Krzysztof Smarzyński, Joanna Le Thanh-Blicharz, Miroslava Kačániová, Hanna Maria Baranowska. LF NMR spectroscopy analysis of water dynamics and texture of Gluten-Free bread with cricket powder during storage. Food Science and Technology International. 2021; ():1.

Chicago/Turabian Style

Przemysław Łukasz Kowalczewski; Katarzyna Walkowiak; Łukasz Masewicz; Krzysztof Smarzyński; Joanna Le Thanh-Blicharz; Miroslava Kačániová; Hanna Maria Baranowska. 2021. "LF NMR spectroscopy analysis of water dynamics and texture of Gluten-Free bread with cricket powder during storage." Food Science and Technology International , no. : 1.

Journal article
Published: 01 January 2021 in Open Life Sciences
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Leaf rust caused by the fungus Puccinia recondita f. sp. tritici is one of the most dangerous diseases of common wheat. Infections caused by fungal pathogens reduce the quantity and quality of yields of many cereal species. The most effective method to limit plant infection is to use cultivars that show rust resistance. Genetically conditioned horizontal-type resistance (racial-nonspecific) is a desirable trait because it is characterized by more stable expression compared to major (R) genes that induce racially specific resistance, often overcome by pathogens. Horizontal resistance is conditioned by the presence of slow rust genes, which include genes Lr34 and Lr46. This study aimed to identify markers linked to both genes in 64 common wheat lines and to develop multiplex PCR reaction conditions that were applied to identify both genes simultaneously. The degree of infestation of the analyzed lines was also assessed in field conditions during the growing season of 2017 and 2018. Simple sequence repeat anchored-polymerase chain reaction (SSR-PCR) marker csLV was identified during analysis in line PHR 4947. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. In addition to gene Lr34, gene Lr46 was identified in this genotype. Lines PHR 4947 and PHR 4819 were characterized by the highest leaf rust resistance in field conditions. During STS-PCR analyses, the marker wmc44 of gene Lr46 was identified in most of the analyzed lines. This marker was not present in the following genotypes: PHR 4670, PHR 4800, PHR 4859, PHR 4907, PHR 4922, PHR 4949, PHR 4957, PHR 4995, and PHR 4997. The presence of a specific sequence has also been confirmed in multiplex PCR analyses. Genotypes carrying the markers of the analyzed gene showed good resistance to leaf rust in field conditions in both 2017 and 2018. Research has demonstrated that marker assisted selection (MAS) and multiplex PCR techniques are excellent tools for selecting genotypes resistant to leaf rust.

ACS Style

Agnieszka Tomkowiak; Roksana Skowrońska; Michał Kwiatek; Julia Spychała; Dorota Weigt; Danuta Kurasiak-Popowska; Janetta Niemann; Sylwia Mikołajczyk; Jerzy Nawracała; Przemysław Łukasz Kowalczewski; Kinza Khan. Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR. Open Life Sciences 2021, 16, 172 -183.

AMA Style

Agnieszka Tomkowiak, Roksana Skowrońska, Michał Kwiatek, Julia Spychała, Dorota Weigt, Danuta Kurasiak-Popowska, Janetta Niemann, Sylwia Mikołajczyk, Jerzy Nawracała, Przemysław Łukasz Kowalczewski, Kinza Khan. Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR. Open Life Sciences. 2021; 16 (1):172-183.

Chicago/Turabian Style

Agnieszka Tomkowiak; Roksana Skowrońska; Michał Kwiatek; Julia Spychała; Dorota Weigt; Danuta Kurasiak-Popowska; Janetta Niemann; Sylwia Mikołajczyk; Jerzy Nawracała; Przemysław Łukasz Kowalczewski; Kinza Khan. 2021. "Identification of leaf rust resistance genes Lr34 and Lr46 in common wheat (Triticum aestivum L. ssp. aestivum) lines of different origin using multiplex PCR." Open Life Sciences 16, no. 1: 172-183.

Journal article
Published: 01 January 2021 in Open Life Sciences
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“Bryndza” cheese is an important Slovak traditional regional product. New knowledge on the role of microorganisms involved the “Bryndza” ripening process may provide valuable data on its quality and safety. In our study, the “Bryndza” made from pasteurized ewes milk was studied towards total count of bacteria, coliforms bacteria, enterococci, lactic acid bacteria, and microscopic filamentous fungi. All those groups of microbiota were detected using classical microbiological methods and identified using mass spectrometry. A total of 3,758 isolates were identified with score higher than 2.00. Altogether, 13 families, 24 genus, and 44 species of microbiota were identified in Slovak cheese “Bryndza.” The most often isolated species were yeasts Yarrowia lipolitica and Dipodascus geotrichum and the lactic acid bacteria Lactobacillus paracasei subsp. paracasei.

ACS Style

Miroslava Kačániová; Margarita Terentjeva; Simona Kunová; Peter Haščík; Przemysław Łukasz Kowalczewski; Jana Štefániková. Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”. Open Life Sciences 2021, 16, 277 -286.

AMA Style

Miroslava Kačániová, Margarita Terentjeva, Simona Kunová, Peter Haščík, Przemysław Łukasz Kowalczewski, Jana Štefániková. Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”. Open Life Sciences. 2021; 16 (1):277-286.

Chicago/Turabian Style

Miroslava Kačániová; Margarita Terentjeva; Simona Kunová; Peter Haščík; Przemysław Łukasz Kowalczewski; Jana Štefániková. 2021. "Diversity of microbiota in Slovak summer ewes’ cheese “Bryndza”." Open Life Sciences 16, no. 1: 277-286.

Journal article
Published: 31 December 2020 in Applied Rheology
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The current study was designed to enhance the functionality of white bread by replacement of wheat flour with different levels (1%, 2%, 5%, and 8%) of grape seeds micropowder (GSMP) with nanosized particles (10 µm). Chemical composition of GSMP, volume and sensory attributes, evaluated with the panel of evaluators and an electronic nose (e-nose) and an electronic eye (e-eye) were investigated in the tested breads. It has been found out that GSMP contained appreciable amounts of flavonoids including catechin, epicatechin, gallic acid and minerals especially, Ca, K and Mg. The data from rheological analysis showed that the addition of GSMP (mainly at 5% and 8% levels) to the wheat flour had a positive effect on dough manifesting with rheology by increased dough stability. The volume of the experimental breads (above 1% concentration) was demonstrably declined (P < 0.0001) in comparison with the control bread. Sensory rating revealed that the bread fortified with 1% GSMP was judged by the consumer panelists as the most acceptable with the highest scores for all quality attributes which was also confirmed by the data of e-nose and e-eye. Our results suggest for the first time that 1% GSMP addition appears to be a promising functional ingredient to improve bread with required qualitative and sensory properties.

ACS Style

Veronika Valková; Hana Ďúranová; Jana Štefániková; Michal Miškeje; Marián Tokár; Lucia Gabríny; Przemysław Łukasz Kowalczewski; Miroslava Kačániová. Wheat Bread with Grape Seeds Micropowder: Impact on Dough Rheology and Bread Properties. Applied Rheology 2020, 30, 138 -150.

AMA Style

Veronika Valková, Hana Ďúranová, Jana Štefániková, Michal Miškeje, Marián Tokár, Lucia Gabríny, Przemysław Łukasz Kowalczewski, Miroslava Kačániová. Wheat Bread with Grape Seeds Micropowder: Impact on Dough Rheology and Bread Properties. Applied Rheology. 2020; 30 (1):138-150.

Chicago/Turabian Style

Veronika Valková; Hana Ďúranová; Jana Štefániková; Michal Miškeje; Marián Tokár; Lucia Gabríny; Przemysław Łukasz Kowalczewski; Miroslava Kačániová. 2020. "Wheat Bread with Grape Seeds Micropowder: Impact on Dough Rheology and Bread Properties." Applied Rheology 30, no. 1: 138-150.

Journal article
Published: 31 October 2020 in Antibiotics
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Staphylococcus spp. is not only a commensal bacteria but also a major human pathogen that causes a wide range of clinical infections. Recent evidence suggests that Staphylococcus has the ability to colonize the reproductive system and to affect its structure and functions. The objective of this study was to determine the chemical properties and antibacterial effects of select essential oils (EOs): Amyris balsamifera L., Boswellia carterii Birdw., Canarium luzonicum (Blume) A. Gray, Cinnamomum camphora (L.) J. Presl., Cinnamomum camphora var. linaloolifera Y. Fuita, Citrus x aurantium L., Gaultheria procumbens L., Litsea cubeba (Lour.) Pers., Melaleuca ericifolia Smith., Melaleuca leucadendra L., Pogostemon cablin (Blanco) Benth., Citrus limon (L.) Osbeck, Santalum album L., and Vetiveria zizanoides (L.) Roberty against 50 Staphylococcus spp. cultures isolated from human semen, specifically Staphylococcus aureus, S. capiti, S. epidermidis, S. haemoliticus, and S. hominis. The disc diffusion and broth microdilution methods were used to assess the antimicrobial potential and to determine the minimum inhibitory concentration (MIC) of the selected EOs. The best anti-Staphylococcus activities were found with both methods for the essential oils of C. luzonicum (Blume) A. Gray, A. balsamifera, C. camphora, and P. cabli.

ACS Style

Miroslava Kačániová; Margarita Terentjeva; Jana Štefániková; Jana Žiarovská; Tatsiana Savitskaya; Dmitrij Grinshpan; Przemysław Kowalczewski; Nenad Vukovic; Eva Tvrdá. Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen. Antibiotics 2020, 9, 765 .

AMA Style

Miroslava Kačániová, Margarita Terentjeva, Jana Štefániková, Jana Žiarovská, Tatsiana Savitskaya, Dmitrij Grinshpan, Przemysław Kowalczewski, Nenad Vukovic, Eva Tvrdá. Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen. Antibiotics. 2020; 9 (11):765.

Chicago/Turabian Style

Miroslava Kačániová; Margarita Terentjeva; Jana Štefániková; Jana Žiarovská; Tatsiana Savitskaya; Dmitrij Grinshpan; Przemysław Kowalczewski; Nenad Vukovic; Eva Tvrdá. 2020. "Chemical Composition and Antimicrobial Activity of Selected Essential Oils against Staphylococcus spp. Isolated from Human Semen." Antibiotics 9, no. 11: 765.

Journal article
Published: 14 September 2020 in International Journal of Molecular Sciences
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The aim of study was to isolate and identify the gut bacteria of Apis mellifera and to evaluate antagonistic effect of the bacteriota against Paenibacillus larvae, which causes American foulbrood (AFB) in honeybees. The dilution plating method was used for the quantification of selected microbial groups from digestive tract of bees, with an emphasis on the bacteriota of the bees’ intestines. Bacteria were identified using mass spectrometry (MALDI-TOF-MS Biotyper). Overall, five classes, 27 genera and 66 species of bacteria were identified. Genera Lactobacillus (10 species) and Bacillus (8 species) were the most abundant. Gram-negative bacteria were represented with 16 genera, whereas Gram-positive with 10 genera. Delftia acidovorans and Escherichia coli were the most abundant in the digestive tract of honey bee. Resistance to a selection of antimicrobials was assessed for the bacterial isolates from bee gut and confirmed against all antimicrobials included in the study, with the exception of cefepime. Lactobacillus spp., especially L. kunkeei, L. crispatus and L. acidophilus. showed the strongest antimicrobial activity against P. larvae, the causal pathogen of AFB. Antimicrobial activity of essential oils against isolated bacteria and two isolates of P. larvae were assessed. Application of a broad selection of plant essential oils indicated that Thymus vulgaris had the highest antimicrobial activity against P. larvae.

ACS Style

Miroslava Kačániová; Margarita Terentjeva; Jana Žiarovská; Przemysław Łukasz Kowalczewski. In Vitro Antagonistic Effect of Gut Bacteriota Isolated From Indigenous Honey Bees and Essential Oils Against Paenibacillus Larvae. International Journal of Molecular Sciences 2020, 21, 6736 .

AMA Style

Miroslava Kačániová, Margarita Terentjeva, Jana Žiarovská, Przemysław Łukasz Kowalczewski. In Vitro Antagonistic Effect of Gut Bacteriota Isolated From Indigenous Honey Bees and Essential Oils Against Paenibacillus Larvae. International Journal of Molecular Sciences. 2020; 21 (18):6736.

Chicago/Turabian Style

Miroslava Kačániová; Margarita Terentjeva; Jana Žiarovská; Przemysław Łukasz Kowalczewski. 2020. "In Vitro Antagonistic Effect of Gut Bacteriota Isolated From Indigenous Honey Bees and Essential Oils Against Paenibacillus Larvae." International Journal of Molecular Sciences 21, no. 18: 6736.

Journal article
Published: 01 September 2020 in Agronomy
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In the grain samples of three malting barley varieties harvested in 2016, and in the malt made from them, we observed microbial changes during storage. Analyses were performed after 0, 3, 6, and 9 months of storage in floor warehouses and silos. We monitored microbial changes based on the quantitative determination of microorganisms using a plate dilution method and qualitative determination of microorganisms, where we identified a particular species of bacteria using the MALDI-TOF MS Biotyper instrument (Bruker Daltonics, Bremen, Germany). Based on the quantitative determination of microorganisms, we found that the total plate counts of microorganisms was higher in malts than in barley grains. The storage period had the greatest impact on the number of coliform bacteria, which gradually increased with the growing months in storage. The number of lactic acid bacteria in barley grains with the growing months in storage and malt produced from them decreased slightly, and the content of the sporulating bacteria in all the samples fluctuated slightly. Differences in the numbers of observed microorganisms were negligible during storage between floor warehouses and silos. In the samples of barleys, the most commonly identified species were Pantoea agglomerans, which was also present in malt samples but did not belong to the dominant species. A diverse species representation appeared in the samples, whereas predominating species belonged to the Enterobacteriaceae family.

ACS Style

Soňa Felšöciová; Przemysław Łukasz Kowalczewski; Tomáš Krajčovič; Štefan Dráb; Miroslava Kačániová. Quantitative and Qualitative Composition of Bacterial Communities of Malting Barley Grain and Malt during Long-Term Storage. Agronomy 2020, 10, 1301 .

AMA Style

Soňa Felšöciová, Przemysław Łukasz Kowalczewski, Tomáš Krajčovič, Štefan Dráb, Miroslava Kačániová. Quantitative and Qualitative Composition of Bacterial Communities of Malting Barley Grain and Malt during Long-Term Storage. Agronomy. 2020; 10 (9):1301.

Chicago/Turabian Style

Soňa Felšöciová; Przemysław Łukasz Kowalczewski; Tomáš Krajčovič; Štefan Dráb; Miroslava Kačániová. 2020. "Quantitative and Qualitative Composition of Bacterial Communities of Malting Barley Grain and Malt during Long-Term Storage." Agronomy 10, no. 9: 1301.

Journal article
Published: 30 August 2020 in Molecules
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The main aim of the study was to investigate the chemical composition, antioxidant, antimicrobial, and antibiofilm activity of Citrus aurantium essential oil (CAEO). The biofilm profile of Stenotrophonomonas maltophilia and Bacillus subtilis were assessed using the mass spectrometry MALDI-TOF MS Biotyper and the antibiofilm activity of Citrus aurantium (CAEO) was studied on wood and glass surfaces. A semi-quantitative composition using a modified version was applied for the CAEO characterization. The antioxidant activity of CAEO was determined using the DPPH method. The antimicrobial activity was analyzed by disc diffusion for two biofilm producing bacteria, while the vapor phase was used for three penicillia. The antibiofilm activity was observed with the agar microdilution method. The molecular differences of biofilm formation on different days were analyzed, and the genetic similarity was studied with dendrograms constructed from MSP spectra to illustrate the grouping profiles of S. maltophilia and B. subtilis. A differentiated branch was obtained for early growth variants of S. maltophilia for planktonic cells and all experimental groups. The time span can be reported for the grouping pattern of B. subtilis preferentially when comparing to the media matrix, but without clear differences among variants. Furthermore, the minimum inhibitory doses of the CAEO were investigated against microscopic fungi. The results showed that CAEO was most active against Penicillium crustosum, in the vapor phase, on bread and carrot in situ.

ACS Style

Miroslava Kačániová; Margarita Terentjeva; Lucia Galovičová; Eva Ivanišová; Jana Štefániková; Veronika Valková; Petra Borotová; Przemysław Kowalczewski; Simona Kunová; Soňa Felšöciová; Eva Tvrdá; Jana Žiarovská; Renáta Benda Prokeinová; Nenad Vukovic. Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model. Molecules 2020, 25, 3956 .

AMA Style

Miroslava Kačániová, Margarita Terentjeva, Lucia Galovičová, Eva Ivanišová, Jana Štefániková, Veronika Valková, Petra Borotová, Przemysław Kowalczewski, Simona Kunová, Soňa Felšöciová, Eva Tvrdá, Jana Žiarovská, Renáta Benda Prokeinová, Nenad Vukovic. Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model. Molecules. 2020; 25 (17):3956.

Chicago/Turabian Style

Miroslava Kačániová; Margarita Terentjeva; Lucia Galovičová; Eva Ivanišová; Jana Štefániková; Veronika Valková; Petra Borotová; Przemysław Kowalczewski; Simona Kunová; Soňa Felšöciová; Eva Tvrdá; Jana Žiarovská; Renáta Benda Prokeinová; Nenad Vukovic. 2020. "Biological Activity and Antibiofilm Molecular Profile of Citrus aurantium Essential Oil and Its Application in a Food Model." Molecules 25, no. 17: 3956.

Journal article
Published: 11 August 2020 in Molecules
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The biological activity of chocolates gains more and more attention of consumers. Its antioxidant properties depend, among other factors, mainly on the origin of cocoa and the characteristics that this origin gives to the final product. Therefore, the aim of the study was to measure and compare the total content of polyphenols, antioxidant activity, and key odorants of commercial chocolates made from blend cocoa with single-origin ones. The highest content of polyphenols was found in 90% blend cocoa chocolate and single-origin samples, while the lowest content was exhibited by 100% chocolate from blend cocoa mass. The highest antioxidant activity measured by 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) and ferric reducing antioxidant power (FRAP) assays was observed in the sample of chocolate with 90% cocoa solids from blend mass, followed by single-origin chocolates. A high positive correlation between ABTS assay and the total polyphenol and phenolic acids’ content, as well as among the total content of polyphenols, flavonoids, and phenolic acids was found. Mineral composition analysis showed that dark chocolate is a valuable source of some elements, especially Mg, Fe, and Zn. Potentially toxic elements were not detected or below permitted limits. Moreover, it was noticed that the main volatile compound in all tested samples was acetic acid, but pyrazines were considered the most important group of chocolate odorants.

ACS Style

Lucia Godočiková; Eva Ivanišová; Grzegorz Zaguła; Luis Noguera-Artiaga; Ángel A. Carbonell-Barrachina; Przemysław Łukasz Kowalczewski; Miroslava Kačániová. Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates. Molecules 2020, 25, 3648 .

AMA Style

Lucia Godočiková, Eva Ivanišová, Grzegorz Zaguła, Luis Noguera-Artiaga, Ángel A. Carbonell-Barrachina, Przemysław Łukasz Kowalczewski, Miroslava Kačániová. Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates. Molecules. 2020; 25 (16):3648.

Chicago/Turabian Style

Lucia Godočiková; Eva Ivanišová; Grzegorz Zaguła; Luis Noguera-Artiaga; Ángel A. Carbonell-Barrachina; Przemysław Łukasz Kowalczewski; Miroslava Kačániová. 2020. "Antioxidant Activities and Volatile Flavor Components of Selected Single-Origin and Blend Chocolates." Molecules 25, no. 16: 3648.

Journal article
Published: 29 June 2020 in Open Chemistry
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This article focused on the content of lycopene in fresh and dried tomatoes and tomato pomace, as well as in tomato paste at different harvest times (harvest 1 – August and harvest 2 – September). The lycopene content of tomatoes and tomato products was evaluated by using high-performance liquid chromatography (HPLC). The results showed that the highest content of lycopene was estimated in the tomato paste independent of the time of harvest (211.73 mg/100 g dm in August and 184.29 mg/100 g dm in September) and the lowest content in fresh pomace (20.45 and 16.11 mg/100 g dm in August and September, respectively). Good sources of lycopene are tomato by-products, in particularly dried tomato pomace (25.11 mg/100 g dm – harvest 1 and 19.30 mg/100 g dm – harvest 2). This study showed that tomato waste can be considered as a promising source of lycopene for the production of functional foods.

ACS Style

Danuta Górecka; Agata Wawrzyniak; Anna Jędrusek-Golińska; Krzysztof Dziedzic; Jadwiga Hamułka; Przemysław Łukasz Kowalczewski; Jarosław Walkowiak. Lycopene in tomatoes and tomato products. Open Chemistry 2020, 18, 752 -756.

AMA Style

Danuta Górecka, Agata Wawrzyniak, Anna Jędrusek-Golińska, Krzysztof Dziedzic, Jadwiga Hamułka, Przemysław Łukasz Kowalczewski, Jarosław Walkowiak. Lycopene in tomatoes and tomato products. Open Chemistry. 2020; 18 (1):752-756.

Chicago/Turabian Style

Danuta Górecka; Agata Wawrzyniak; Anna Jędrusek-Golińska; Krzysztof Dziedzic; Jadwiga Hamułka; Przemysław Łukasz Kowalczewski; Jarosław Walkowiak. 2020. "Lycopene in tomatoes and tomato products." Open Chemistry 18, no. 1: 752-756.

Journal article
Published: 10 June 2020 in Molecules
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In this study, two saponins-rich plant extracts, viz. Saponaria officinalis and Quillaja saponaria, were used as surfactants in an oil-in-water (O/W) emulsion based on hempseed oil (HSO). This study focused on a low oil phase content of 2% v/v HSO to investigate stable emulsion systems under minimum oil phase conditions. Emulsion stability was characterized by the emulsification index (EI), centrifugation tests, droplet size distribution as well as microscopic imaging. The smallest droplets recorded by dynamic light scattering (droplets size v. number), one day after the preparation of the emulsion, were around 50–120 nm depending the on use of Saponaria and Quillaja as a surfactant and corresponding to critical micelle concentration (CMC) in the range 0–2 g/L. The surface and interfacial tension of the emulsion components were studied as well. The effect of emulsions on environmental bacteria strains was also investigated. It was observed that emulsions with Saponaria officinalis extract exhibited slight toxic activity (the cell metabolic activity reduced to 80%), in contrast to Quillaja emulsion, which induced Pseudomonas fluorescens ATCC 17400 growth. The highest-stability samples were those with doubled CMC concentration. The presented results demonstrate a possible use of oil emulsions based on plant extract rich in saponins for the food industry, biomedical and cosmetics applications, and nanoemulsion preparations.

ACS Style

Maciej Jarzębski; Przemysław Siejak; Wojciech Smułek; Farahnaz Fathordoobady; Yigong Guo; Jarosław Pawlicz; Tomasz Trzeciak; Przemysław Łukasz Kowalczewski; David D. Kitts; Anika Singh; Anubhav Pratap Singh. Plant Extracts Containing Saponins Affects the Stability and Biological Activity of Hempseed Oil Emulsion System. Molecules 2020, 25, 1 .

AMA Style

Maciej Jarzębski, Przemysław Siejak, Wojciech Smułek, Farahnaz Fathordoobady, Yigong Guo, Jarosław Pawlicz, Tomasz Trzeciak, Przemysław Łukasz Kowalczewski, David D. Kitts, Anika Singh, Anubhav Pratap Singh. Plant Extracts Containing Saponins Affects the Stability and Biological Activity of Hempseed Oil Emulsion System. Molecules. 2020; 25 (11):1.

Chicago/Turabian Style

Maciej Jarzębski; Przemysław Siejak; Wojciech Smułek; Farahnaz Fathordoobady; Yigong Guo; Jarosław Pawlicz; Tomasz Trzeciak; Przemysław Łukasz Kowalczewski; David D. Kitts; Anika Singh; Anubhav Pratap Singh. 2020. "Plant Extracts Containing Saponins Affects the Stability and Biological Activity of Hempseed Oil Emulsion System." Molecules 25, no. 11: 1.

Journal article
Published: 19 May 2020 in Agronomy
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Seed treatments help reduce the pathogen load and thus improve the condition of plants from their earliest developmental stages, but they can have impacts beyond their basic fungicide protection role. In this study, we investigated how seven spring barley seed treatments affected the plants’ physiological state. The tested seed treatments differed significantly in their impacts on the vigor parameters of barley seeds and on the physiological state of seedlings under drought stress and after regeneration. Seed treatments based on substances from the succinate-dehydrogenase-inhibitors (SDHI) group did not cause inhibition of seedling growth and also display by the highest vigor index values. Using the analysis of photosynthesis-related parameters, we showed that seed treatments from the SDHI group provided a superior tolerance of the imposed drought in spring barley than other treatments. In addition to protection against abiotic stress, SDHI treatments also rendered a higher efficiency of photochemical reactions in the treated plants.

ACS Style

Dominika Radzikowska; Monika Grzanka; Przemysław Łukasz Kowalczewski; Romana Głowicka-Wołoszyn; Andrzej Blecharczyk; Marcin Nowicki; Zuzanna Sawinska. Influence of SDHI Seed Treatment on the Physiological Conditions of Spring Barley Seedlings under Drought Stress. Agronomy 2020, 10, 731 .

AMA Style

Dominika Radzikowska, Monika Grzanka, Przemysław Łukasz Kowalczewski, Romana Głowicka-Wołoszyn, Andrzej Blecharczyk, Marcin Nowicki, Zuzanna Sawinska. Influence of SDHI Seed Treatment on the Physiological Conditions of Spring Barley Seedlings under Drought Stress. Agronomy. 2020; 10 (5):731.

Chicago/Turabian Style

Dominika Radzikowska; Monika Grzanka; Przemysław Łukasz Kowalczewski; Romana Głowicka-Wołoszyn; Andrzej Blecharczyk; Marcin Nowicki; Zuzanna Sawinska. 2020. "Influence of SDHI Seed Treatment on the Physiological Conditions of Spring Barley Seedlings under Drought Stress." Agronomy 10, no. 5: 731.