This page has only limited features, please log in for full access.
Onychomycosis is a disease that affects many adults, whose treatment includes both oral and topical therapies with low cure rates. The topical therapy is less effective but causes fewer side effects. This is why the development of an effective, easy to apply formulation for topical treatment is of high importance. We have used a nanotechnological approach to formulate Pickering emulsions (PEs) with well-defined properties to achieve site-specific delivery for antifungal drug combination of tioconazole and Melaleuca alternifolia essential oil. Silica nanoparticles with tailored size and partially hydrophobic surface have been synthesized and used for the stabilization of PEs. In vitro diffusion studies have been performed to evaluate the drug delivery properties of PEs. Ethanolic solution (ES) and conventional emulsions (CE) have been used as reference drug formulations. The examination of the antifungal effect of PEs has been performed on Candida albicans and Trichophyton rubrum as main pathogens. In vitro microbiological experimental results suggest that PEs are better candidates for onychomycosis topical treatment than CE or ES of the examined drugs. The used drugs have shown a significant synergistic effect, and the combination with an effective drug delivery system can result in a promising drug form for the topical treatment of onychomycosis.
Barbara Vörös-Horváth; Sourav Das; Ala’ Salem; Sándor Nagy; Andrea Böszörményi; Tamás Kőszegi; Szilárd Pál; Aleksandar Széchenyi. Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment. Molecules 2020, 25, 5544 .
AMA StyleBarbara Vörös-Horváth, Sourav Das, Ala’ Salem, Sándor Nagy, Andrea Böszörményi, Tamás Kőszegi, Szilárd Pál, Aleksandar Széchenyi. Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment. Molecules. 2020; 25 (23):5544.
Chicago/Turabian StyleBarbara Vörös-Horváth; Sourav Das; Ala’ Salem; Sándor Nagy; Andrea Böszörményi; Tamás Kőszegi; Szilárd Pál; Aleksandar Széchenyi. 2020. "Formulation of Tioconazole and Melaleuca alternifolia Essential Oil Pickering Emulsions for Onychomycosis Topical Treatment." Molecules 25, no. 23: 5544.
The extreme lipophilicity of essential oils (EOs) impedes the measurement of their biological actions in an aqueous environment. We formulated oil in water type Pickering Artemisia annua EO nanoemulsions (AEP) with surface-modified Stöber silica nanoparticles (20 nm) as the stabilizing agent. The antimicrobial activity of AEP and its effects on mature Candida biofilms were compared with those of Tween 80 stabilized emulsion (AET) and ethanolic solution (AEE) of the Artemisia EO. The antimicrobial activity was evaluated by using the minimum inhibitory concentrations (MIC90) and minimum effective concentrations (MEC10) of the compounds. On planktonic bacterial and fungal cells beside growth inhibition, colony formation (CFU/mL), metabolic activity, viability, intracellular ATP/total protein (ATP/TP), along with reactive oxygen species (ROS) were also studied. Artemisia annua EO nanoemulsion (AEP) showed significantly higher antimicrobial activity than AET and AEE. Artemisia annua EO nanoemulsions (AEP) generated superoxide anion and peroxides-related oxidative stress, which might be the underlying mode of action of the Artemisia EO. Unilamellar liposomes, as a cellular model, were used to examine the delivery efficacy of the EO of our tested formulations. We could demonstrate higher effectiveness of AEP in the EO components’ donation compared to AET and AEE. Our data suggest the superiority of the AEP formulation against microbial infections.
Sourav Das; Barbara Vörös-Horváth; Tímea Bencsik; Giuseppe Micalizzi; Luigi Mondello; Györgyi Horváth; Tamás Kőszegi; Aleksandar Széchenyi. Antimicrobial Activity of Different Artemisia Essential Oil Formulations. Molecules 2020, 25, 2390 .
AMA StyleSourav Das, Barbara Vörös-Horváth, Tímea Bencsik, Giuseppe Micalizzi, Luigi Mondello, Györgyi Horváth, Tamás Kőszegi, Aleksandar Széchenyi. Antimicrobial Activity of Different Artemisia Essential Oil Formulations. Molecules. 2020; 25 (10):2390.
Chicago/Turabian StyleSourav Das; Barbara Vörös-Horváth; Tímea Bencsik; Giuseppe Micalizzi; Luigi Mondello; Györgyi Horváth; Tamás Kőszegi; Aleksandar Széchenyi. 2020. "Antimicrobial Activity of Different Artemisia Essential Oil Formulations." Molecules 25, no. 10: 2390.
Medicinal plants are widely used in folk medicine but quite often their composition and biological effects are hardly known. Our study aimed to analyze the composition, cytotoxicity, antimicrobial, antioxidant activity and cellular migration effects of Anthyllis vulneraria, Fuchsia magellanica, Fuchsia triphylla and Lysimachia nummularia used in the Romanian ethnomedicine for wounds. Liquid chromatography with mass spectrometry (LC-MS/MS) was used to analyze 50% (v/v) ethanolic and aqueous extracts of the plants’ leaves. Antimicrobial activities were estimated with a standard microdilution method. The antioxidant properties were evaluated by validated chemical cell-free and biological cell-based assays. Cytotoxic effects were performed on mouse fibroblasts and human keratinocytes with a plate reader-based method assessing intracellular adenosine triphosphate (ATP), nucleic acid and protein contents and also by a flow cytometer-based assay detecting apoptotic–necrotic cell populations. Cell migration to cover cell-free areas was visualized by time-lapse phase-contrast microscopy using standard culture inserts. Fuchsia species showed the strongest cytotoxicity and the highest antioxidant and antimicrobial activity. However, their ethanolic extracts facilitated cell migration, most probably due to their various phenolic acid, flavonoid and anthocyanin derivatives. Our data might serve as a basis for further animal experiments to explore the complex action of Fuchsia species in wound healing assays.
Rita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants 2020, 9, 166 .
AMA StyleRita Csepregi, Viktória Temesfői, Sourav Das, Ágnes Alberti, Csenge Anna Tóth, Róbert Herczeg, Nóra Papp, Tamás Kőszegi. Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants. Antioxidants. 2020; 9 (2):166.
Chicago/Turabian StyleRita Csepregi; Viktória Temesfői; Sourav Das; Ágnes Alberti; Csenge Anna Tóth; Róbert Herczeg; Nóra Papp; Tamás Kőszegi. 2020. "Cytotoxic, Antimicrobial, Antioxidant Properties and Effects on Cell Migration of Phenolic Compounds of Selected Transylvanian Medicinal Plants." Antioxidants 9, no. 2: 166.
We investigated the antifungal activities of purified plant metabolites artemisinin (Ar) and scopoletin (Sc) including inhibition, effects on metabolic activities, viability, and oxidative stress on planktonic forms and on preformed biofilms of seven Candida species. The characteristic minimum inhibitory concentration (MIC90) of Ar and Sc against Candida species ranged from 21.83–142.1 µg/mL and 67.22–119.4 µg/mL, respectively. Drug concentrations causing ≈10% CFU decrease within 60 min of treatments were also determined (minimum effective concentration, MEC10) using 100-fold higher CFUs than in the case of MIC90 studies. Cytotoxic effects on planktonic and on mature biofilms of Candida species at MEC10 concentrations were further evaluated with fluorescent live/dead discrimination techniques. Candida glabrata, Candida guilliermondii, and Candida parapsilosis were the species most sensitive to Ar and Sc. Ar and Sc were also found to promote the accumulation of intracellular reactive oxygen species (ROS) by increasing oxidative stress at their respective MEC10 concentrations against the tested planktonic Candida species. Ar and Sc possess dose-dependent antifungal action but the underlying mechanism type (fungistatic and fungicidal) is not clear yet. Our data suggest that Ar and Sc found in herbal plants might have potential usage in the fight against Candida biofilms.
Sourav Das; Lilla Czuni; Viktória Báló; Gábor Papp; Zoltán Gazdag; Nóra Papp; Tamás Kőszegi; Papp. Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species. Molecules 2020, 25, 476 .
AMA StyleSourav Das, Lilla Czuni, Viktória Báló, Gábor Papp, Zoltán Gazdag, Nóra Papp, Tamás Kőszegi, Papp. Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species. Molecules. 2020; 25 (3):476.
Chicago/Turabian StyleSourav Das; Lilla Czuni; Viktória Báló; Gábor Papp; Zoltán Gazdag; Nóra Papp; Tamás Kőszegi; Papp. 2020. "Cytotoxic Action of Artemisinin and Scopoletin on Planktonic Forms and on Biofilms of Candida Species." Molecules 25, no. 3: 476.
Essential oils (EOs) are highly lipophilic, which makes the measurement of their biological action difficult in an aqueous environment. We formulated a Pickering nanoemulsion of chamomile EO (CPe). Surface-modified Stöber silica nanoparticles (20 nm) were prepared and used as a stabilizing agent of CPe. The antimicrobial activity of CPe was compared with that of emulsion stabilized with Tween 80 (CT80) and ethanolic solution (CEt). The antimicrobial effects were assessed by their minimum inhibitory concentration (MIC90) and minimum effective (MEC10) concentrations. Besides growth inhibition (CFU/mL), the metabolic activity and viability of Gram-positive and Gram-negative bacteria as well as Candida species, in addition to the generation of oxygen free radical species (ROS), were studied. We followed the killing activity of CPe and analyzed the efficiency of the EO delivery for examined formulations by using unilamellar liposomes as a cellular model. CPe showed significantly higher antibacterial and antifungal activities than CT80 and CEt. Chamomile EOs generated superoxide anion and peroxide related oxidative stress which might be the major mode of action of Ch essential oil. We could also demonstrate that CPe was the most effective in donation of the active EO components when compared with CT80 and CEt. Our data suggest that CPe formulation is useful in the fight against microbial infections.
Sourav Das; Barbara Horváth; Silvija Šafranko; Stela Jokić; Aleksandar Széchenyi; Tamás Kőszegi. Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations. Molecules 2019, 24, 4321 .
AMA StyleSourav Das, Barbara Horváth, Silvija Šafranko, Stela Jokić, Aleksandar Széchenyi, Tamás Kőszegi. Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations. Molecules. 2019; 24 (23):4321.
Chicago/Turabian StyleSourav Das; Barbara Horváth; Silvija Šafranko; Stela Jokić; Aleksandar Széchenyi; Tamás Kőszegi. 2019. "Antimicrobial Activity of Chamomile Essential Oil: Effect of Different Formulations." Molecules 24, no. 23: 4321.
Free essential oils and their active components have a low physiochemical stability and low aqueous solubility which limit their applications as food preservatives and in packaging industry. The aim of this study was to characterize the physicochemical properties, antioxidant activities and antimicrobial activity of randomly methylated β cyclodextrin (RAMEB) encapsulated thyme oil, lemon balm oil, lavender oil, peppermint oil and their active components that include thymol, citral, linalool, menthol and borneol. Inclusion complex formation of essential oils (EOs) and RAMEB were evaluated by several methods. Antioxidant capacities of RAMEB-EOs/components were reported to be more stable than free EOs/components (P < 0.05). Rapid SYBR green I/propidium iodide live/dead microbial cellular discrimination assay for Schizosaccharomyces pombe, Escherichia coli and Staphylococcus aureus showed similar results when compared with flow cytometry analysis (P < 0.01) suggesting that our novel microplate fluorescence method could be applied for the fast live/dead microbial discrimination in antimicrobial assays.
Sourav Das; Zoltán Gazdag; Lajos Szente; Mátyás Meggyes; Györgyi Horváth; Beáta Lemli; Sándor Kunsági-Máté; Mónika Kuzma; Tamás Kőszegi. Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils. Food Chemistry 2018, 278, 305 -313.
AMA StyleSourav Das, Zoltán Gazdag, Lajos Szente, Mátyás Meggyes, Györgyi Horváth, Beáta Lemli, Sándor Kunsági-Máté, Mónika Kuzma, Tamás Kőszegi. Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils. Food Chemistry. 2018; 278 ():305-313.
Chicago/Turabian StyleSourav Das; Zoltán Gazdag; Lajos Szente; Mátyás Meggyes; Györgyi Horváth; Beáta Lemli; Sándor Kunsági-Máté; Mónika Kuzma; Tamás Kőszegi. 2018. "Antioxidant and antimicrobial properties of randomly methylated β cyclodextrin – captured essential oils." Food Chemistry 278, no. : 305-313.